Diet-induced obesity (DIO) diets and phenotypes
Research Diets
For several years, we have been collaborating with Research Diets, Inc. to provide DIO mice to the research community. Research Diets offers the widest range of purified diets, harboring constant protein and nutrients, which are used to produce DIO mice. The three most popular DIO diets, D12451i (45 kcal% fat), D12331i - with sucrose (58 kcal% fat), and D12492i (60 kcal% fat), are used extensively by academic, pharmaceutical and biotechnologies institutions.
Table 1. Macro-nutrient composition of commonly used high fat diets from Research Diets, Inc.
| Formula # | D12450B | D12451 | D12492 | |||
|---|---|---|---|---|---|---|
| gm% | kcal% | gm% | kcal% | gm% | kcal% | |
| Protein | 19 | 20 | 24 | 20 | 26 | 20 |
| Carbohydrate | 67 | 70 | 41 | 35 | 26 | 20 |
| Fat | 4 | 10 | 24 | 45 | 35 | 60 |
| Total | 100 | 100 | 100 | |||
| kcal/gm | 3.8 | 4.7 | 5.2 | |||
| Note that the source of the increased fat is from lard. | ||||||
Major diabetes and obesity phenotypes of male B6 mice fed diets with 10, 45, and 60 kcal% fat
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| Figure 1. Mean body weights (g +/- SEM) for groups of B6 males (n=20-44) fed 10, 45, and 60 kcal% fat diets from six to 17 weeks of age. | Figure 2. Mean blood glucose levels (mg/dl +/- SEM) for groups of 17- to 19-week old B6 males (n=10-33) fed 10, 45, and 60 kcal% fat diets for 11-13 weeks (data obtained by glucose tolerance test). |
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Figure 3. Mean % body fat (+/- SEM) for groups of B6 males (n = 10-17) fed 10, 45, and 60 kcal% fat diets for 11-13 weeks (data obtained by DEXA). |
Comparative diabetes and obesity phenotypes of commonly used JAX® diabetes and obesity models
Comparison tables summarizing metabolic disease phenotypes for eight selected, well characterized or emerging JAX Mice models that together comprise a robust tool set for basic research and therapeutic screening.
References
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