Diet-induced obesity (DIO) diet composition

  • Inventoried C57BL/6J DIO mice are maintained on D12492 (60 kcal% fat) and the control diet, D12450B (10 kcal% fat)
  • Customized diets and studies can be designed for C57BL/6J or any other strain

Table 1. Macro-nutrient composition of lard-based high fat inventory DIO diets from Research Diets, Inc.

10 kcal% fat
(inventoried control)
D12450B

60 kcal% fat
(inventoried experimental)
D12492

gm% kcal% gm% kcal%
Protein 19 20 26 20
Carbohydrate 67 70 26 20
Fat 4 10 35 60
Total 100 100
kcal/gm 3.8 5.2
gm kcal gm kcal
Fat from lard 20 180 245 2205

Table 2. Macro-nutrient composition of hydrogenated coconut oil w/sucrose diets from Research Diets, Inc. (custom diets available upon request).

11 kcal% fat
(custom control)
D12329

58 kcal% fat
(custom experimental)
D12331

gm% kcal% gm% kcal%
Protein 17 16 23 16
Carbohydrate 74 73 36 26
Fat 5 11 36 58
Total 100 100
kcal/gm 4.1 5.6
gm kcal gm kcal
Sucrose 835 3340 175 700
Fat from coconut oil 40 360 334 3002