Patented Genetic Stability Program
Our patented Genetic Stability Program (GSP) effectively prevents cumulative genetic drift, including that caused by copy number variation, in our most popular strains.
Our GSP program effectively limits cumulative genetic drift by refreshing our foundation stocks with cryopreserved pedigree embryos every five generations (Fig. 1). The program was initiated in 2003 (Taft et al. 2006) and is covered in patents issued in 2010 (US patent 7,592,501) and 2012 (US patent 8,110,721).
|Figure 1: Foundation stocks of several broadly-used strains are refreshed with cryo-recovered embryos every five generations.|
So far, we have cryopreserved germplasm from the following strains:
- 129S1/SvlmJ (002448)
- B6.129P2-Apoetm1Unc/J (002052)
- C3H/HeJ (000659)
- C57BL/6J (000664)
- C57BL/6NJ (005304)
- DBA/2J (000671)
- FVB/NJ (001800)
- NOD/ShiLtJ (001976)
- NOD.CB17-Prkdcscid/J (001303)
- BALB/cByJ (001026)
- CBA/J (000656)
- DBA/1J (000670)
Preventing cumulative genetic drift in these strains is particularly important because they have been either completely or partly sequenced: the C57BL/6J strain was completely sequenced by the Mouse Genome Sequencing Consortium (Waterston et al. 2002); the DBA/2J strain was partly sequenced by Celera Genomics (Marshall 2001); and the FVB/NJ, BALB/cByJ, and 13 other JAX® Mice strains have been sequenced by the National Institute of Environmental Health Sciences Resequencing Project (JAX® NOTES 2005).