Overview of The Jackson Laboratory facility barrier levels
Low barrier: microisolator caging (non-ventilated or individually ventilated); pasteurized or sterilized feed; sterilized bedding; gown; gloves; restricted entry
RAF Standard barrier: microisolator caging (non-ventilated or individually ventilated); pasteurized or sterilized feed; sterilized bedding; gown, gloves, mask, cap, and sterilized shoes/shoe covers; restricted entry
Standard barrier: microisolator caging (non-ventilated or individually ventilated); sterilized feed and bedding; change into sterilized clothing and shoes; gloves, mask, cap; restricted entry
High barrier: microisolator caging (non-ventilated or individually ventilated); sterilized feed and bedding; sterilization or disinfection of all supplies and equipment; air or wet shower during first entry of the day; change into sterilized clothing and shoes; gloves; mask, cap; restricted entry
Maximum barrier: microisolator caging (non-ventilated or individually ventilated); sterilized feed and bedding; sterilization or disinfection of all supplies and equipment; wet shower and change into sterilized clothing and shoes at each entry; gloves; mask, cap; restricted entry
Isolator: all mice maintained in isolators; all supplies and equipment sterilized
