Inventoried DIO Mice Now Available
JAX® NOTES Issue 505, Spring 2007
JAX® Mice and Services now distributes 6- to 26-week old diet-induced obese (DIO) C57BL/6J (B6, 000664) males, the most widely used models of diet-induced obesity. Age-matched mice fed a control diet are also available. Using this convenient and time-saving new service, researchers can begin experiments involving DIO mice immediately, without tying up valuable research time and mouse room space to pre-condition them.
To produce inventoried DIO mice, four-week old B6 males are randomly selected from our colonies and fed a 6% chow diet (Lab Diet® 5k52). When they are 6 weeks old, they are placed in wean cages in groups of 10, fed either the study diet (Research Diets, Inc. D12492i, 60 kcal% fat) or the control diet (Research Diets, Inc. D12450Bi, 10 kcal% fat), and given ad libitum access to food and water. They are weighed when 6, 12, and 18 weeks old but are not individually identified or marked. These mice become obese, mildly to moderately hyperglycemic, and develop impaired glucose tolerance. The graphs below depict the typical weight gained by and oral glucose tolerance test responses of these mice.
You may order inventoried DIO mice by e-mail (DIOemail@jax.org) or by phone (1-800-422-MICE (6423)). JAX® Services can ship them to you or to our In Vivo Services group, where they will be used to conduct studies according to your specifications.
DIO mice can also be produced using your diet of preference through JAX® Diet-Induced Obesity Service. For more information about JAX® Diet-Induced Obesity Service or Inventoried DIO Mice (including the most recent health report for the room where DIO mice are housed), please visit the Web site.
Fig. 1 Mean body weights (g+/- SEM) for groups of B6 males (n=20-44) fed 10% and 60% diets from 6 to 17 weeks of age.
Fig. 2 Mean blood glucose levels (mg/dl+/- SEM) for groups of 17- to 19- week old B6 males (n=10-33) fed 10% and 60% fat diets for 11-13 weeks (data obtained by glucose tolerance test.)
