Newly Available JAX® Mice Strains
JAX® NOTES Issue 510, Summer 2008
Below is a partial list of newly available JAX® Mice strains. For a complete list, see Newly Available Strains.
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CByJ.B6-Tg(UBC-GFP)30Scha/J 007076
This transgenic strain expresses enhanced green fluorescent protein (EGFP) under the direction of the human ubiqutin C promoter. EGFP is expressed in all tissues examined. Certain hematopoietic cell types display distinct expression levels, allowing researchers to identify different cells types by FACS analysis. This strain may be used to research hematopoietic cell differentiation and for tracking leukocytes in vivo.
B6.129S2(C)-Il8rbtm1Mwm/J 006848
This strain harbors a targeted mutation of the interleukin 8 receptor, beta (Il8rb) gene. Homozygotes have several abnormalities, including neurological defects, impaired wound healing, impaired angiogenesis, and altered growth of induced implanted tumors. Homozygotes may also exhibit splenomegaly, lymphadenopathy, and increased susceptibility to various pathogens due to impaired neutrophil recruitment and decreased pathogen clearance during innate immune responses. This strain may be used to research inflammation, immunology, and cancer biology.
B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J 006230
Mice homozygous for this floxed allele of the CAAT/enhancer binding protein (C/EBP), alpha gene and hemizygous for th Mx1-cre transgene are viable, fertile, and have a normal hematopoietic system. Mx1-Cre expression can be induced by administering either interferon (alpha or beta) or synthetic double-stranded RNA (such as poly I:C), which deletes the floxed gene. Following 3-4.5 weeks of poly I:C treatment, deletion efficiency is greater than 95% in hematopoietic tissues, and C/EBPalpha protein is undetectable in bone marrow. Poly I:Ctreated, Cebpa-deleted, Mx1-cre mice have defective myeloid cell development, increased hematopoietic stem cell repopulating activity, and a significantly increased myeloblast population in the bone marrow compartment. This strain may be used to research hematopoietic cell (e.g. myeloid and basophil progenitor cell) development and function, cancer (e.g. acute myeloid leukemia), and alveolar cell differentiation.
B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J 006911
Mice heterozygous for both targeted mutations (R26-rtTA and Cola1a::tetO-Oct4) are viable and fertile. They express rtTA-M2, an optimized form of reverse tetracycline-controlled transactivator (rtTA) protein, in multiple tissues. In the absence of the tetracycline analog doxycycline (dox), Oct4 (Pou5f1) expression from the Col1a1 locus is not detected. Following dox administration, high Oct4 expression is induced in liver, bone marrow, stomach, intestine, and skin, with lower levels in the heart, lungs, kidney, spleen, and thymus. No expression was detected in the brain and testes. Dox-inducd activation of Oct4 results in dysplasia in epithelial tissues. This strain may be used to research tumorigenesis and pluripotent cells.
CB6-Tg(Gad1-EGFP)G42Zjh/J 007677
Hemizygotes for this GAD67-GFP transgene express enhanced green fluorescent protein (EGFP) in the calcium-binding protein parvalbumin (Pv)-expressing subclass of basket interneurons (soma, dendrites, and axons) and also in putative presynaptic boutons. This strain may be useful for fluorescent labeling of the Pv-expressing subset of GABAergic neurons, allowing reliable and efficient characterization of perisomatic innervation in vivo.
B6.Cg-Park7tm1Shn/J 006577
Homozygotes for this targeted mutation of the Parkinson disease (autosomal recessive, early onset) 7 (Park7) gene are viable and fertile. Homozygotes exhibit hypokinesia and nigrostriatal dopaminergic deficits: evoked dopamine overflow in the striatum is reduced (primarily as a result of increased dopamine uptake); nigral neurons (dopaminergic neurons) have abnormal action potential characteristics; and long term depression is absent in medium spiny neurons. Dopaminergic neurons from substantia nigra pars compacta (SNpc) of homozygotes exhibit significantly higher sensitivity to energy metabolism impairment, and nigral dopaminergic neurons are particularly sensitive to Na+/ K+ ATPase impairment. This strain may be used to research Parkinson's disease, dopaminergic physiology, nigrostriatal function, locomotor inactivity, and other neurobiological phenotypes.
B6.129-Gt(ROSA)26Sortm3Luo/J 006075
This strain is designed for MADM (mosaic analysis with double markers) and must be crossed to one harboring a reciprocal mutation at the same locus (see Stock No. 006067 or Stock No. 006080, MADM-R (Dsred2/EGFP)). The resulting offspring have one copy of each reciprocal mutation on homologous chromosomes ("transheterozygous") and must next be bred to a cre-expressing strain for fluorescent protein expression. Using this MADM system, a researcher can generate genetic mosaics in which an individual organism contains somatic cells of different genotypes. This allows the researcher to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation and mitosis.
B6;129S4-Mc4rtm1Lowl/J 006414
The mice of this strain have a loxP-flanked transcriptional blocking (loxTB) sequence that prevents normal transcription and translation of the endogenous melanocortin 4 receptor (Mc4r) gene. They exhibit severe early-onset obesity, accompanied by hyperphagia, increased body length, and hyperinsulinemia. Mcr4 function can be restored by the enzymatic activity of Cre-recombinase. This strain may be used to research neurobiology, obesity, diabetes, hunger/appetite, and fat and energy metabolism.
B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J 006361
Homozygotes for this Osx1-GFP::Cre transgene are viable and fertile. The transgene carries both tTA under the regulation of the osterix (Sp7) promoter and, just downstream, a tetracycline responsive element (TRE; tetO)-controlled GFP/Cre fusion protein. In the absence of the tetracycline analog doxycycline, EGFP-Cre fusion protein expression is restricted to the osteoblast lineage throughout embryonic and early postnatal development. When this strain is mated to transgenic strains with loxP-flanked (floxed) conditional alleles, doxycycline-induced double mutant offspring will have no conditional deletion and no fusion protein expression; removal of doxycycline results in deletion of the floxed allele as well as fusion protein expression. The donating investigator suggests that this strain be maintained on dox treated water to avoid incidental effects of tTA expression. This strain may be used to research bone development, osteoblast lineage, and Hedgehog/Wnt signaling.
STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J 007576
The mT/mG strain possesses loxP sites on either side of a membrane-targeted tdTomato (mT) cassette and express red fluorescence in all tissues and cell types examined. When bred to Cre recombinase expressing mice, the mT cassette is deleted in the cre expressing tissue(s), allowing expression of the membrane-targeted EGFP (mG) cassette located just downstream. This double-fluorescent system allows direct live visualization of both recombined and non-recombined cells at single cell resolution, offering an internal control for phenotypic analysis of Cre-induced mosaic mutants and providing a second marker for lineage tracing applications. In addition, the localization of fluorescent proteins to membrane structures outlines cell morphology and allows resolution of fine cellular processes. This strain may be used as a Cre reporter strain, expressing red fluorescence before, and green fluorescence after, Cre-mediated recombination in widespread cell and tissue types.
FVB/N-Tg(Tagln-rtTA)E1Jwst/J 006875
This strain expresses the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the murine SM22-alpha (SM22α or transgelin) promoter. When hemizygotes are mated to a transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE; tetO), they produce offspring in which expression of the target gene is inducible in smooth muscle cells by administering the tetracycline analog, doxycycline. This strain is a "Tet-On" tool that allows the inducible expression of genes in smooth muscle cells.
