The Jackson Laboratory

Home > JAX^® Mice & Services > News & events > JAX^® NOTES > Issue 513

Newly Available JAX^® Mice strains

JAX^® NOTES Issue 513, Spring 2009

Targeted Mutants

B6.Cg-Dicer1^tm1Bdh/J 006366

This Dicer1 strain contains loxP sites flanking exon 23 of the Dcr-1 homolog (Drosophila) gene. It can be used to generate cell/tissue-specific deletions of the endogenous gene for applications in embryonic development, translation, protein processing, and miRNA/siRNA regulation of gene expression.

B6.129S6(FVB)-Ptgs2^tm1.1Fun/J 008101

This strain has an amino acid substitution that inactivates the cyclooxygenase but not the peroxidase activity of the enzyme. Homozygotes exhibit abnormal kidney function and elevated systolic blood pressure. This strain may be used to research thrombogenesis and hypertension.

B6.Cg-Mirn155^tm1.1Rsky/J 007745

This bic/mir-155 strain may be used to study the role of miR-155 and its target genes (including those encoding cytokines, chemokines and transcription factors) in homeostasis and functions of the immune system.

B6;129S4-Pou5f1^tm1Jae/J 008204

This Oct4-neo mutant strain may be used to select induced pluripotent stem (iPS) cells.

B6;129S4-Pou5f1^tm2Jae/J 008214

This Oct4-EGFP mutant strain may be used to select induced pluripotent stem (iPS) cells or, more generally, to fluorescently label embryonic stem cells.

STOCK Gli1^{tm3(cre/ESR1)Alj}/J 007913

When this Gli1-CreER^T2 strain is bred with one containing a loxP-flanked sequence of interest, it produces offspring in which tamoxifen-inducible, Cre-mediated recombination deletes the flanked sequences in Gli1-expressing cells. The strain may be used to study axis patterning, proliferation, and cell fate specification of hedgehog-responding cells at different stages of embryogenesis.

Transgenic Strains

B6.Cg-Tg(CAG-Ub*G76V/GFP)1Dant/J 008111
B6.Cg-Tg(CAG-Ub*G76V/GFP)2Dant/J 008112

Both ubiquitin/proteasome system reporter lines, UbG76V-GFP/1 (008111) and UbG76V-GFP/2 (008112), may be used to monitor the role of ubiquitin/proteasome-dependent proteolysis in diverse disorders, and to test the efficacy of compounds on the ubiquitin/proteasome system.

B6.Cg-Tg(tetO-cre)1Jaw/J 006234
FVB.Cg-Tg(tetO-cre)1Jaw/J 008244

Transgenic tetO-cre mice express Cre recombinase under the control of a tetracycline-responsive promoter element (TRE or tetO). When bred with a transgenic strain expressing a reverse tetracycline-controlled transactivator protein, or (r)tTA, they produce bitransgenic offspring in which Cre-mediated recombination can be regulated with the tetracycline analog doxycycline. These strains are an effective tool for generating inducible, tissue-specific-targeted mutants for studying cell lineage during development.

C57BL/6-Tg(Thy1-APPSwDutIowa)BWevn/J 007027

This transgenic strain expresses the human amyloid beta-precursor protein, APP gene, 770 isoform, with the Swedish K670N/M671L, Dutch E693Q and Iowa D694N mutations, under the direction of the mouse thymus cell antigen 1, theta, Thy1, promoter. Plaque-like deposits of amyloid beta, similar to those observed in people with the Dutch and Iowa familial disorders, are initially detected at approximately three months in the subiculum, hippocampus and cortex. Amyloid beta deposits are detected throughout the forebrain by 12 months. The strain may be used to study Alzheimer's.

C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J 007567

CD11c-Cre-GFP transgenic mice may be used to study the immune system or protein expression in dendritic cells.

STOCK Tg(tetO-DTA)1Gfi/J 008168

This tet-DTA strain expresses diphtheria toxin A (DTA) under the control of a tetracycline operator (tetO or TRE) and a cytomegalovirus minimal promoter. It may be used to generate bi-transgenic mutant mice for the reversible, inducible deletion of specific groups of cells.