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Newly Available JAX Mice Strains

JAX^® NOTES Issue 516, Winter 2010

STOCK Tg(RARE-Hspa1b/lacZ)12Jrt/J 008477

This transgenic strain expresses beta-galactosidase (lacZ) gene under the control of the retinoic acid responsive element (RARE). Retinoic acid signaling is indicated by beta‑galactosidase activity. This strain may be used to track retinoic acid signaling patterns.

B6.129P2-Myd88^tm1Defr/J 008888

Mice of this strain have a conditional (floxed) knockout allele of myeloid differentiation primary response gene 88 (Myd88) and may be used to research Toll-like receptor signaling.

B6.129P2-Lgr5^{tm1(cre/ESR1)Cle}/J 008875

This knockin strain expresses both EGFP and a Cre-ERT2 fusion protein from the endogenous Lgr5 (leucine rich repeat containing G protein coupled receptor 5) promoter, in place of the Lgr5 coding sequence. It may be used to mark Lgr5‑expressing stem cells of the small intestine, pre-malignant adenomas, colon cancer cells, epithelial stem cells of the stomach gland, basal epithelial stem cells of the mammary glands, and hair follicle stem cells.

129-Wnt11^tm1Amc/J 006239

This strain harbors a knockout mutation of the Wnt11 (wingless-related MMTV integration site 11) gene and may be used to study kidney development and Wnt superfamily functions during embryogenesis.

B6.Cg-Tg(Nlrp1b)1Die/DieJ 006840

This BAC transgenic strain expresses the mouse NLR family pyrin domain (Nlrp1b). It may be used to research inflammatory autoimmune and infectious diseases.

B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J 008344

This bi-transgenic TetTag strain features tetracycline (or tetracycline analog) inducible expression of beta-galactosidase in activated neurons.

B6;129-Gt(ROSA)26Sor^rtm1Joe/J 008516

Once an upstream loxP-flanked STOP sequence is removed, this ROSA26 GNZ knockin strain expresess a nuclear-localized green fluorescent protein/beta-galactosidase fusion protein (GFP-NLS-lacZ or GNZ). When bred to a cre expressing strain, it produces offspring that express the GNZ fusion protein in cells where Cre recombinase is active.

B6;129S6-Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/J 007905

B6.Cg-Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/J 007909

These two Ai9 strains harbor a targeted mutation of the Gt(ROSA)26Sor locus with a loxP-flanked STOP cassette, which prevents the transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato). They may be used as Cre reporter strains.

STOCK Tg(Thy1-EGFP)MJrs/J 007788

This Thy1-GFPM transgenic strain may be used in neurobiological studies to fluorescently label neural tissues, especially mossy fibers in the cerebellum and a variety of other neuronal subsets.

B6.129P2-Gt(ROSA)26Sor^{tm1(Trpv1,ECFP)Mde}/J 008513

In this knockin strain, a loxP-flanked neomycin cassette blocks expression of the rat Trpv1 (transient receptor potential cation channel, subfamily V, member 1) gene from the Gt(ROSA)26Sor promoter. When crossed to a tissue-specific Cre-expressing strain, it produces offspring that express TRPV1 and enhanced cyan fluorescent protein (ECFP). Cells expressing TRPV1 are sensitive to capsaicin and similar chemical agonists. Treating the mice or cells that have undergone Cre recombination induces strong inward currents, triggers action potentials, and activates stereotyped behaviors, allowing cell‑type specific chemical genetic control of neuronal activity in vitro and in vivo.

C57BL/6-Arg1^tm1Pmu/J 008817

This Arg1^flox mutant strain (Arg1, arginase 1, liver) may be used to generate conditional mutations to study argininemia and the immune response to bacterial and parasitic infections.