Search Criteria: Strain Type is "Transgenic"
| Stock Number |
Strain Name Strain Description |
Standard Supply |
| 002726 | B6SJL-Tg(SOD1*G93A)1Gur/J | Level 3 |
| Mice hemizygous for this SOD1-G93A (also called G93A-SOD1) transgene are viable and fertile, with transgenic expression of a G93A mutant form of human SOD1. This founder line (often referred to as G1H) is reported to have high transgene copy number. Hemizygotes exhibit a phenotype similar to amyotrophic lateral sclerosis (ALS) in humans; becoming paralyzed in one or more limbs with paralysis due to loss of motor neurons from the spinal cord. Transgenic mice have an abbreviated life span: 50% survive at 128.9+/-9.1 days (in contrast to C57BL/6J background where 50% survival is observed at 157.1+/-9.3 days). These SOD1-G93A (also called G93A-SOD1) transgenic mice may be useful in studying neuromuscular disorders, including Amyotrophic Lateral Sclerosis (ALS or Lou Gehrig's Disease). | ||
| 003303 | C.Cg-Tg(DO11.10)10Dlo/J | Level 3 |
| Mice carrying the MHC class II restricted rearranged T cell receptor transgene, Tg(DO11.10)10Dlo, react to ovalbumin (OVA) peptide antigen. Intraperitoneal administration of OVA to transgenic mice results in a rapid deletion of the immature CD4+ CD8+ TCRlo thymocytes with progression to mature thymocytes. Apoptosis of cortical thymocytes within 20 hours of treatment indicates that apoptosis in important in the development of antigen-induced tolerance. Use of this rearranged T cell receptor transgene requires H2d background. | ||
| 004194 | B6.Cg-Tg(TcraTcrb)425Cbn/J | Level 4 |
| These transgenic mice express the mouse alpha-chain and beta-chain T cell receptor that pairs with the CD4 coreceptor and is specific for chicken ovalbumin 323-339 in the context of I-A b. Homozygous mice are viable and fertile. In these mice there is a four-fold increase in the CD4 to CD8 peripheral T cell ratio, and lymph node T cells demonstrate a dose-dependent proliferative response to the specific ovalbumin ligand. These transgenic mice are useful for studying in vivo T cell biology such as TCR-ligand interactions, T cell activation, thymic selection, cross-presentation of antigens, process of thymic selection and central and peripheral T cell tolerance and induction. | ||
| 002810 | B6CBA-Tg(HDexon1)62Gpb/1J | Level 4 |
| This line is transgenic for the 5' end of the human HD gene carrying approximately 160 +/- 10 repeat expansions. The transgene is ubiquitously expressed. Transgenic mice exhibit a progressive neurological phenotype that mimics many of the features of HD, including choreiform-like movements, involuntary stereotypic movements, tremor, and epileptic seizures, as well as nonmovement disorder components, including unusual vocalization. They urinate frequently and exhibit loss of body weight and muscle bulk through the course of the disease. Neurologically they develop Neuronal Intranuclear Inclusions (NII) which contain both the huntingtin and ubiquitin proteins. Previously unknown, these NII have subsequently been identified in human HD patients. The age of onset of HD symptoms is reported to occur between nine and 11 weeks. Commonly known as the "R6/2" strain.
Transgenic mice develop hyperglycemia by 12 weeks of age with a corresponding decrease in insulin levels. Pancreatic beta cell ..... | ||
| 006494 | B6CBA-Tg(HDexon1)62Gpb/3J | Level 4 |
| This line is transgenic for the 5' end of the human HD gene carrying approximately 120 +/- 5 (CAG)repeat expansions. The transgene is ubiquitously expressed. Transgenic mice exhibit a progressive neurological phenotype that mimics many of the features of HD, including choreiform-like movements, involuntary stereotypic movements, tremor, and epileptic seizures, as well as nonmovement disorder components, including unusual vocalization. They urinate frequently and exhibit loss of body weight and muscle bulk through the course of the disease. Neurologically they develop Neuronal Intranuclear Inclusions (NII) which contain both the huntingtin and ubiquitin proteins. Previously unknown, these NII have subsequently been identified in human HD patients. The age of onset of HD symptoms is reported to occur between 9 and 11 weeks. Commonly known as the "R6/2" strain. Transgenic mice develop hyperglycemia by 12 weeks of age with a corresponding decrease in insulin levels. Pancreatic beta cel ..... | ||
| 001927 | C57BL/6-Tg(APOA1)1Rub/J | Level 4 |
| Mice carrying the human apolipoprotein A-I transgene show a two fold increase in total plasma cholesterol levels and greater than a four fold decrease in the levels of mouse apoAI. Homozygous transgenic mice exhibit reduced susceptibility to diet induced fatty streak lesions. Mice homozygous for the transgenic insert are viable, fertile and normal in size. This human apolipoprotein AI transgene is under the control of its natural promoter. The Jackson Laboratory imported mice derived from the founder line A2 as described in Rubin EM, et al., 1991. | ||
| 003291 | C57BL/6-Tg(CAG-EGFP)1Osb/J | Level 4 |
| This transgenic mouse line with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer makes all of the tissues, with the exception of erythrocytes and hair, appear green under excitation light. Note that the donating investigator reports that mice homozygous for this transgene die within the first two weeks following birth.
Of note, it has been the experience at The Jackson Laboratory that Stock No. 006567 (C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ) demonstrates the highest proportion of GFP expressing cell populations in bone marrow, thymus, spleen and peripheral blood when compared to Stock No. 003291 (C57BL/6-Tg(CAG-EGFP)1Osb/J) and Stock No. 007075 (CByJ.B6-Tg(CAG-EGFP)1Osb/J).
View the pdf document on For more information please see the full phenotype on the strain data sheet | ||
| 003475 | C57BL/6-Tg(HLA-A2.1)1Enge/J | Level 4 |
| Homozygous mice carrying the Tg(HLA-A2.1)1Enge transgene express significant quantities of the human class I MHC Ag HLA-A2.1 on cells from the spleen, bone marrow and thymus. Expression of this human class I molecule did not result in expansion of the number of cytotoxic T lymphocyte (CTL) precursors specific for other human class I Ag, HLA-B27 or HLA-A2.2. These transgenic mice have been used to identify hepatitic C virus (HCV) peptides expressing a sequence for HLA-A2.1 binding that are actually recognized by human A2.1-restricted CTLs. Thus, this transgenic model is important for the study of HLA-restricted CTL determinants and in potential development of a vaccine against HCV. *Note: copy number may be variable. Hemizygotes are tested for expression prior to distribution. | ||
| 003831 | C57BL/6-Tg(TcraTcrb)1100Mjb/J | Level 4 |
| These mice contain transgenic inserts for mouse Tcra-V2 and Tcrb-V5 genes. The transgenic T cell receptor was designed to recognize ovalbumin residues 257-264 in the context of H2Kb and used to study the role of peptides in positive selection and the response of CD8+ T cells to antigen. Like most TCR transgenics, these mice are somewhat immunodeficient. | ||
| 004353 | C57BL/6-Tg(UBC-GFP)30Scha/J | Level 4 |
| These transgenic mice express enhanced Green Fluorescent Protein (GFP) under the direction of the human ubiqutin C promoter. Mice homozygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice express GFP in all tissues examined. Certain hematapoetic cell types display distinct expression levels of GFP, allowing identification of different cells types by FACS analysis. GFP expression is uniform within a cell type lineage and remains constant throughout development. T cells have a 2-fold higher GFP expression than CD19+B220+ B cells or peripheral blood cells. Leukocytes and red blood cells from homozygous mice fluoresce at approximately twice the level of cells from hemizygous mice. This mutant mouse strain represents a useful tool in studies related to hematopoetic cell differentiation and in vivo tracking of leukocytes. | ||
| 002376 | FVB/N-Tg(MMTVneu)202Mul/J | Level 4 |
| Mice homozygous for the MMTVneu (rat) transgene are viable and fertile. There is no phenotypic effect in males. The transgene is expressed at low levels in normal mammary epithelium, salivary gland, and lung. Higher expression is detected in tumor tissue. Focal mammary tumors first appear at 4 months, with a median incidence of 205 days. Both virgin and breeder mice develop tumors. Tumors arise as foci in hyperplastic, dysplastic mammary glands. Seventy-two percent of tumor-bearing mice that live to 8 months or longer develop metastatic disease to the lung. The phenotype of MMTV/unactivated neu transgenic mice differs from that of the MMTV/activated neu produced by Phil Leder, in which multifocal tumors involving the entire mammary epithelium arise. | ||
| 008215 | (C57BL/6-Tg(TRAMP)8247Ng/J x FVB/NJ)F1/J | Repository- Live |
| Mice carrying the TRAMP (Transgenic Adenocarcinoma of Mouse Prostate) transgene develop progressive forms of prostate cancer with distant site metastasis, primarily to the lymph nodes and lungs. These transgenic mice express the simian virus 40 (SV40) large tumor T antigen (Tag) under the control of the rat probasin promoter. Expression of the transgene is specific to the prostate epithelium. Transgenic mice exhibit various forms of disease from mild intraepithelial hyperplasia to large multinodular malignant neoplasia. The median survival time for these F1 transgenic mice is 19 weeks, very few mice survive past 33 weeks of age, which is significantly shorter than the lifespan of transgenic mice on the C57BL/6 background. Comparative histological analysis of tumors from these F1 transgenic mice and from transgenic mice on the C57BL/6 background reveals that the tumors found in these F1 mutants arise from the dorsolateral and ventral lobes of the prostate and are more spherical, hig ..... For more information please see the full phenotype on the strain data sheet | ||
| 016251 | 129S.Cg-Tg(Hoxb7-EGFP)33Cos/J | Repository- Live |
| Hoxb7-EGFP transgenic mice have the homeobox B7 promoter/enhancer sequences driving expression of an enhanced green fluorescent protein, EGFP. Hemizygotes are viable, fertile, and normal in size. Under control of Hoxb7, EGFP labels the Wolffian duct and branching ureteric bud (UB) of the kidney during embryonic development. Fluorescence is evident in tissues specific to the UB lineage in adult and newborn kidneys, including the ureter, pelvis, and collecting ducts. This strain allows for the visualization of tissues specific to the UB lineage. In contrast, mice containing the Tg(Hoxb7-Venus*)17Cos allele (Stock No. 016252) are useful for viewing individual cells. These mice may be useful for visualizing UB growth and branching in vivo or in vitro. | ||
| 016567 | 129S.Cg-Tg(Hoxb7-rtTA*M2)2Cos/J | Repository- Live |
| RS-HTA2 transgenic mice have the homeobox B7 promoter/enhancer sequences driving expression of an optimized form of the reverse tetracycline-controlled transactivator (rtTA*M2) protein. Hemizygotes are viable, fertile, and normal in size. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the gene of interest may be regulated by the tetracycline analog, doxycycline (dox). In the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. When bred to B6;SJL-Tg(tetop-lacZ)2Mam/J mice (Stock No. 002621), adult mice carrying both transgenes, which were maintained on Dox during pre and postnatal life, show strong expression of βgal in the renal collecting duct system, and embryos display strong expression throughout the Wolffian duct, ureteric bud, vas deferens, epididymis ..... For more information please see the full phenotype on the strain data sheet | ||
| 007179 | 129S.Cg-Tg(UBC-cre/ERT2)1Ejb/J | Repository- Live |
| Mice hemizygous for this Cre-ERT2 transgene are viable and fertile. Mice from this founder line have strong tamoxifen-inducible cre activity in all reported tissue types. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to OHT. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in widespread cel ..... For more information please see the full phenotype on the strain data sheet | ||
| 007915 | 129S.FVB-Tg(Amh-cre)8815Reb/J | Repository- Live |
| Mice harboring the Amh-cre transgene are viable and fertile, with expression of Cre recombinase directed by the testis Sertoli cell-specific promoter elements of the anti-Mullerian hormone (Amh) gene. Cre-recombinase activity is reported in testis Sertoli cells during male sexual development as early as E14.5, with no evidence for cre expression detected in other tissues examined. When Amh-cre transgenic males are bred with female mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence specifically in testis Sertoli cells. These Amh-cre transgenic mice may be useful in generating conditional knockouts in testis Sertoli cells for studying male embryonic sexual differentiation and the regulation of spermatogenesis. | ||
| 003328 | 129S/Sv-Tg(Prm-cre)58Og/J | Repository- Live |
| Mice homozygous for this PrmCre transgene are viable and fertile. Embryonic stem cells containing recombinase transgenes that are expressed in the male germ line, but not in other tissues or in the embryonic stem cells themselves, substantially simplify the production of subtle or conditional mutations in mice. This strain shows that transgenes comprised of the mouse protamine 1 promoter and the Cre recombinase coding sequence mediate the efficient recombination of a cre target transgene in the male germ line, but not in other tissues. This system can be used for reducing the time, effort, and resources required to produce homologously recombined alleles in mice that have been secondarily rearranged by site-specific recombinase. | ||
| 007853 | 129S/SvEv-Tg(Alb1-Ren)2Unc/CofJ | Repository- Live |
| Mice heterozygous for the RenTgMK transgene are viable and fertile. The RenTgMK transgene contains a liver-specific albumin promoter/enhancer controlling the expression of a synthetic renin gene (engineered to allow efficient cleavage and secretion of the prorenin transgene product by the liver). This, and because the transgene was targeted to the apolipoprotein locus on Chromosome 9, results in active renin secretion from the liver independently of renal or other homeostatic cardiovascular control mechanisms (i.e. genetically clamped). RenTgMK heterozygotes have high ectopic levels of active mouse renin in the liver and elevated plasma levels of prorenin and active renin. Heterozygous mice display significantly elevated blood pressure, enhanced thirst, high urine output, proteinuria, and kidney damage. Because active renin overexpression results in increased circulating levels of angiotensin II (Ang II), heterozygotes also exhibit cardiac hypertrophy and 50% male mortality between 6-8 ..... For more information please see the full phenotype on the strain data sheet | ||
| 002761 | B10.Cg-Tg(TcrAND)53Hed/J | Repository- Live |
| Mice carrying the (TcrAND)53Hed transgene express a rearranged T-cell receptor (V alpha 11.1 / V beta 3) specific for the carboxy-terminal fragment of pigeon cytochrome c and the Ek molecule, resulting in a major subpopulation of T cells restricted to class II MHC proteins. There are an abnormally high percentage of mature CD4+CD8- cells. The peripheral T-cell population is almost exclusively CD4+. The original C57BL/6 and SJL mixed background strain (Stock number 002408) was backcrossed to C57BL/10 to create this strain. Both strains are fixed for H2b. Because C57BL/10 mice do not express I-E, this mouse must be crossed to a strain that expresses I-Ek to study the interaction of the transgenic T-cell receptor with the pigeon cytochrome c antigen. The lack of I-Ek expression in the transgenic line allows it to serve as a universal donor for crossing the transgene onto other strains expressing I-Ek<> ..... For more information please see the full phenotype on the strain data sheet | ||
| 009575 | B6(129S4)-Et(cre/ERT2)119Rdav/J | Repository- Live |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of the Cre-ERT2 fusion gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Cre recombinase activity is observed in brain tissues following tamoxifen administration. The donating investigators may not have assessed expression in tissues other than brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
The Cre-ERT2 fusion protein (Cre-ERT2) consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OH ..... | ||
| 009581 | B6(129S4)-Et(cre/ERT2)1642Rdav/J | Repository- Live |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of the Cre-ERT2 fusion gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Cre recombinase activity is observed in brain tissues following tamoxifen administration. The donating investigators may not have assessed expression in tissues other than brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
For characterization information, see images at the Allen Institute for Brain Science website (Et(cre/ERT2)1642Rdav images). The Cre-ERT2 fusion protein (Cre-ERT2) consists of Cre re ..... | ||
| 009582 | B6(129S4)-Et(cre/ERT2)1645Rdav/J | Repository- Live |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of the Cre-ERT2 fusion gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Cre recombinase activity is observed in brain tissues following tamoxifen administration. The donating investigators may not have assessed expression in tissues other than brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
The Cre-ERT2 fusion protein (Cre-ERT2) consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OH ..... | ||
| 009585 | B6(129S4)-Et(cre/ERT2)2047Rdav/J | Repository- Live |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of the Cre-ERT2 fusion gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Cre recombinase activity is observed in brain tissues following tamoxifen administration. The donating investigators may not have assessed expression in tissues other than brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
The Cre-ERT2 fusion protein (Cre-ERT2) consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OH ..... | ||
| 009577 | B6(129S4)-Et(cre/ERT2)296Rdav/J | Repository- Live |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of the Cre-ERT2 fusion gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Cre recombinase activity is observed in brain tissues following tamoxifen administration. The donating investigators may not have assessed expression in tissues other than brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
For characterization information, see images at the Allen Institute for Brain Science website (Et(cre/ERT2)296Rdav images). The Cre-ERT2 fusion protein (Cre-ERT2) consists of Cre re ..... | ||
| 012687 | B6(129S4)-Tg(SYN1-icre/mRFP1)9934Rdav/J | Repository- Live |
| Mice hemizygous for this lentiviral transgene are viable and fertile, with expression of a codon-improved Cre recombinase/monomeric red fluorescent protein fusion protein (iCre/mRFP1) under control of the human synapsin I promoter. The donating investigator reports Cre recombinase activity in brain tissues as widespread, with no staining in other tested tissues. The donating investigator did not characterize fluorescent expression of the mRFP1 portion of the fusion protein. When these transgenic mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring. | ||
| 011065 | B6(C3)-Tg(Pgk1-FLPo)10Sykr/J | Repository- Live |
| These transgenic mice express the mouse codon-optimized FLP recombinase under the direction of the mouse Pgk1, phosphoglycerate kinase 1, promoter. Transgene expression is detected in testis, ovary, brain, heart, liver and muscle by RT-PCR. When crossed with a strain containing a FRT site-flanked sequence of interest, FLPo recombinase activity is detected in all cells with complete recombinase mediated excision of the target. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. | ||
| 004178 | B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J | Repository- Live |
| These Z/EG transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being liver and lung tissue. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with enhanced GFP expression in tissues expressing Cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells.
As an example, when crossed to a strain expressing Cre recombinase in olfactory sensory neurons (see Stock No. 006668), this mutant mouse strain may be useful in lineage tracing. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved t ..... | ||
| 004218 | B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J | Repository- Live |
| Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Notable exceptions to this phenotype are erythrocytes and adipocytes in which fluorescence is negligible or absent. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available. | ||
| 004146 | B6.129-Tg(Pcp2-cre)2Mpin/J | Repository- Live |
| These transgenic mice express a cre gene inserted into exon 4 of a Pcp2 gene. Mice homozygous for the insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity is observed in most Purkinje cells and some retinal bipolar neurons. Small amounts of activity are observed in an unidentified population of cells of the central nervous system tissue. Recombination is first observed around postnatal day 6 and is fully established 2 to 3 weeks after birth.
View cre expression characterization. | ||
| 016187 | B6.BTBR-Tg(Per1-luc,Per1)1Jt/J | Repository- Live |
| This compound transgenic strain was created by co-injecting a BAC clone encoding the entire mouse Per1 locus and a transgenic vector in which the Per1 promoter drives expression of luciferase (Per1:luc). The expression of Per1:luc oscillates in the suprachiasmatic nuclei (SCN) as well as peripheral tissues in ex vivo cultures (unlike most other Per1:luc transgenic strains which have weak rhythms in peripheral tissues). | ||
| 006054 | B6.C-Tg(CMV-cre)1Cgn/J | Repository- Live |
| In this transgenic strain, deletion of loxP-flanked genes occurs in all tissues, including germ cells. The cre gene in this strain is under the transcriptional control of a human cytomegalovirus minimal promoter and is likely to be expressed before implantation during early embryogenesis. It also appears that the cre gene is X-linked since transgene transmission through males is restricted to female offspring. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available. | ||
| 003904 | B6.CBA-Tg(CETP)5203Tall/J | Repository- Live |
| Mice carrying the human CETP minigene under the control of its own promoter show a marked increase in CETP mRNA in liver when mice are fed a high fat, high cholesterol diet. Less dramatic increases are observed in spleen, small intestine and adipose tissues. Elevated plasma CETP protein levels are also observed. Mice carrying human CETP show reduced levels of plasma high density lipoprotein. This strain has been a useful model in studies related to cholesterol metabolism. | ||
| 008599 | B6.Cg-Cyp1a2/Cyp1a1tm2Dwn Ahrd Tg(CYP1A1,CYP1A2)1Dwn/DwnJ | Repository- Live |
| These "humanized" hCYP1A1_1A2_Cyp1a1/1a2(-/-)_Ahrd mice carry the human CYP1A1 and CYP1A2 genes in the absence of functional mouse Cyp1a1 and Cyp1a2 orthologs, and also mimic the human poor-affinity aryl hydrocarbon receptor (AHR) by carrying the poor-affinity Ahrd allele derived from DBA/2J mice (rather than the high-affinity Ahrb1 allele normally present on a C57BL/6J genetic background); all on a C57BL/6J (reported >99.8%) genetic background. Mice homozygous for the Cyp1a2/Cyp1a1 targeted allele [Cyp1a1/1a2(-/-)], homozygous for the Ahrd allele, and carrying the hCYP1A1_1A2 transgene are viable and fertile with normal lifespan. As the Cyp1a2/Cyp1a1(-) targeted allele lacks the coding regions of both Cyp1a1 and Cyp1a2 genes, no mouse CYP1A1 or CYP1A2 mRNA expression is observed in liver, lung or kidney. Transgene expression of the orth ..... | ||
| 010506 | B6.Cg-Fcer1atm1Knt Tg(FCER1A)1Bhk/J | Repository- Live |
| These double mutant mice express the human Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide, (FCER1A), under the control of the human FCER1A promoter and carry the Fcer1atm1Knt targeted mutation. Mice that are hemizygous for the transgene and homozygous for the targeted mutation express a functioning chimeric receptor complex in which the human alpha-chain associates with the mouse beta- and gamma- chains. Transgene expression is detected on bone marrow derived cultured mast cells (BMMC), mast cells, basophils, monocytes, eosinophils, and epidermal Langerhans cells by FACS, hIgE binding and Western blot analysis and mimics both the human FCER1A expression and cell specificity pattern. The humanized receptor is estimated to be expressed approximately 3 to 5 fold higher than the endogenous mouse receptor. The double mutant mice respond to experimental induction of anaphylaxis and are more sensitive to 2,4,6-tri-nitrobenzenesulfonic aci ..... For more information please see the full phenotype on the strain data sheet | ||
| 004919 | B6.Cg-Fcgrttm1Dcr Tg(CAG-FCGRT)276Dcr/DcrJ | Repository- Live |
| These FcRn-/- hFcRn (276) Tg mice harbor a null mutation of the FcRn α-chain (Fcgrttm1Dcr) and also express a human FcRn α-chain cDNA (FCGRT) under the control the human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter (CAG).
Mice homozygous for the FcRn α-chain null allele lack FcRn α-chain mRNA or protein expression, fail to transport IgG across the neonatal gut, degrade IgG at an accelerated rate, are functionally IgG deficient, and also exhibit plasma albumin deficiency. While hFcRn (276) transgene expression corrects the relative albumin deficiency caused by the FcRn α-chain knockout, expression of the hFcRn (276) transgene is unable to bind and protect murine IgG from degradation in FcRn α-chain null mice. However, hFcRn (276) transgene expression corrects the rapid decay rate of infused human IgG in FcRn-/- hFcRn (276) Tg mice. Furthermore, human antibodies ..... For more information please see the full phenotype on the strain data sheet | ||
| 014565 | B6.Cg-Fcgrttm1Dcr Tg(FCGRT)32Dcr/DcrJ | Repository- Live |
| These mFcRn-/- hFcRn (32) Tg mice are homozygous for the null mutation of the FcRn α-chain (Fcgrttm1Dcr) and express a human FcRn α-chain (FCGRT) transgene. Founder line 32 is more efficient in prolonging the serum half-life of hIgG compared with founder line 276 (see STOCK no. 004919). In Petkova et al.(2006), it is shown that the antibody half life of Hu4D5-IgG1 antibodies in mFcRn-/- hFcRn (32) Tg/0 is similar to those observed in mFcRn-/- hFcRn (276) Tg/Tg mice (see STOCK no. 004919). (Hu4D5-IgG1 human antibodies are directed against human epidermal growth factor receptor 2). In studies by Petkova et al. (2006), mice were pre-treated with human IgG to partially saturate the human FcRn. Since human FcRn does not bind mouse IgG, these mice have low levels of mouse IgG. These humanized FcRn mice may be useful in studying the quantitati ..... For more information please see the full phenotype on the strain data sheet | ||
| 012910 | B6.Cg-Fxntm1Mkn Tg(FXN)YG22Pook/J | Repository- Live |
| The YG22 transgenic founder line carries a single copy of the human FXN gene with one GAA trinucleotide repeat sequence of 190 repeats. High levels of human FXN gene product (mRNA and protein) are detected by RT-PCR and Western blot analysis. 40-50% of the endogenous mouse Fxn gene product (protein) is detected by Western blot analysis in mice heterozygous for the targeted mutation alone. Mice that are homozygous for the targeted allele and hemizygous for the transgene exhibit an age dependent, tissue specific expansion of the GAA repeat, with expansion accumulation in the CNS (particularly cerebellum), similar to the human pathology. The GAA triplet repeat exhibits intergenerational instability. Mice that are homozygous for the targeted allele and hemizygous for the transgene exhibit progressive retinal degeneration, impaired and decreased locomotor activity and coordination, and an increase in body weight. At 9 months of age, muscle strength is decreased. Al ..... For more information please see the full phenotype on the strain data sheet | ||
| 012253 | B6.Cg-Fxntm1Mkn Tg(FXN)YG8Pook/J | Repository- Live |
| Mice that are heterozygous for the targeted allele and hemizygous for the transgene are viable and fertile. High levels of human FXN gene product (mRNA or protein) are detected by RT-PCR and Western blot analysis. 40-50% of the endogenous mouse Fxn gene product (protein) is detected by Western blot analysis in mice heterozygous for the targeted mutation alone. The YG8 transgenic founder carries two tandem copies of the human FXN gene with two GAA triplet repeat sequences of 82 and 190 repeats. Mice that are homozygous for the targeted allele and hemizygous for the transgene exhibit an age dependent, tissue specific expansion of the GAA repeat, with expansion accumulation in the CNS (particularly cerebellum), similar to the human pathology. The GAA triplet repeat exhibits intergenerational instability.
This mutant mouse strain may be useful in studies of Friedreich's Ataxia. In an attempt to offer alleles on well-characterized or multiple genetic backgrou ..... | ||
| 016101 | B6.Cg-Il7rtm1Imx Tg(Lck-Il7r)1676Trma/J | Repository- Live |
| These transgenic mice express the mouse Il7r (interleukin-7 receptor) gene under the control of the proximal mouse Lck (lymphocyte protein tyrosine kinase) promoter, and are homozygous for the Il7rtm1Imx allele. Transgene expression is detected in the thymus, specifically in T cells and thymocytes. No transgene expression is detected in B cells, NK cells, monocytes or in bone marrow. Transgene expression is reduced in CD3-CD4-CD8- (triple negative) thymocytes. The phenotype of mice homozygous for the Il7rtm1Imx allele is substantially rescued by expression of the transgenic wildtype Il7r in the thymus. Transgenic IL-7Rα protein is expressed in most thymocytes at levels similar to those seen in wildtype controls. The number of thymic cells is restored to 74% of wildtype control number. Although T cell development is substantially restored, peripheral T cells express minimal IL-7Ra (reduced by >90%) and are poorly ..... For more information please see the full phenotype on the strain data sheet | ||
| 005491 | B6.Cg-Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J | Repository- Live |
| Mice that are homozygous for the targeted allele and hemizygous for the transgene are viable and fertile. Although no endogenous mouse MAPT is detected, all six isoforms (including both 3R and 4R forms) of human MAPT are expressed. Hyperphosphorylated MAPT is detected in cell bodies and dendrites by three months of age. Paired helical filaments of aggregated insoluble MAPT can be isolated from brain tissue as early as two months of age. These mutant mice may be useful in studies examining the relationship between human MAPT and Alzheimer's disease pathogenesis In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available. | ||
| 008684 | B6.Cg-Rag1tm1Mom Tyrp1B-w Tg(Tcra,Tcrb)9Rest/J | Repository- Live |
| These mice are hemizygous for the TRP-1 transgene Tg(Tcra,Tcrb)9Rest, homozygous for the targeted mutation Rag1tm1Mom and homozygous for the white based brown radiation induced mutation of tyrosinase-related protein 1, Tyrp1B-w. These mutant mice express a MHC class II-restricted TCR recognizing the endogenous melanocyte differentiation antigen minimal TRP-1 (tyrosinase-related protein 1) epitope corresponding to amino acids 113 to 127. The transgene is located on the Y chromosome in founder line 9. On a RAG-deficient background, these mice are also homozygous for the Tyrp1B-w mutation and do not produce endogenous tyrosinase-related protein 1. This strain is a source for melanocyte reactive CD4+ cells from antigen-negative animals and may be useful in studies of cancer immunology and therapeutics. | ||
| 005023 | B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J | Repository- Live |
| This transgenic strain carries a rearranged T cell receptor transgene specific for the mouse homologue (pmel-17) of human SILV (gp100), an enzyme involved in pigment synthesis that is expressed by the majority of malignant melanoma cells including B16 melanoma, as well as by normal melanocytes. The strain is also homozygous for the T lymphocyte specific Thy1a (Thy1.1) allele. CD8+ T cells express a Tcra-V1/Tcrb-V13- transgenic TCR that recognizes an epitope of pmel-17 corresponding to amino acids 25-33 of gp100 presented by H2-Db MHC class I molecules. Greater than 95% of the CD8+ T cells in transgenic mice expressed the transgenic TCR based on the expression of Vbeta13, amounting to about 20% of all splenocytes. T cells in blood and spleen generally expressed baseline levels of the activation/effector markers CD25, CD44, and CD69, indicating that most of the transgenic cells were in the naive state. These transgenic mice in conjunction with the poor ..... For more information please see the full phenotype on the strain data sheet | ||
| 007484 | B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ | Repository- Live |
| On an albino background the X-linked transgene Tg(Tyr)3412ARpw permits visual identification of XX versus XY as early as embryonic day 10.5. This strain is segregating for Tg(Tyr)3412ARpw and homozygous for Tyrc-2J so the individuals not carrying Tg(Tyr3412)ARpw are albino. Because Tg(Tyr)3412ARpw inserted into the X Chromosome, breeding a carrier male with a noncarrier (wild-type) female results in embryos in which all XX individuals develop eye pigment, due to the Tg(Tyr)3412ARpw inherited from their father, while all XY individuals have non-pigmented eyes, having inherited a wild-type X Chromosome from their mother.
This strain is also homozygous for Tg(SryEGFP)92Ei. This reporter transgene consists of a 5 prime regulatory segment of the Sry gene driving EGFP. This transgene is expressed in the pre-support cell lineage (pre-sertoli and pre-granulosa cells) of the fetal genital ridge (Albrecht and Eicher, 2001) and in discrete areas the adult male but not ..... | ||
| 006149 | B6.Cg-Tg(ACTA1-cre)79Jme/J | Repository- Live |
| Mice hemizygous for this HSA-Cre79 transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These HSA-Cre79 transgenic mice have the cre recombinase gene driven by the human alpha-skeletal actin (HSA or ACTA1) promoter. Cre activity is restricted to adult striated muscle fibers and embryonic striated muscle cells of the somites and heart. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in striated muscle-specific deletion of the flanked genome. Specifically, these HSA-Cre79 (or ACTA1-Cre) transgenic mice were originally used to breed with mice heterozygous for a deletion of exon 7 and a loxP-flanked exon 7 mutation on homologous chromosomes of the Smn1 gene (see Stock No. 006138 or Stock No. 006146). The resulting offspring ( ..... For more information please see the full phenotype on the strain data sheet | ||
| 005703 | B6.Cg-Tg(ACTFLPe)9205Dym/J | Repository- Live |
| This transgenic strain expresses a variant of the Saccharomyces cerevisiae FLP1 recombinase gene under the direction of the human ACTB promoter. The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being four-fold and ten-fold that of wildtype FLP at 37oC and 40oC, respectively. Mice that are hemizygous for the transgenic allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5. This deleter strain is a suitable alternative to, and complement with the Cre-loxP system for in vivo genetic engineering.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype coul ..... | ||
| 003574 | B6.Cg-Tg(Alb-cre)21Mgn/J | Repository- Live |
| This strain may be maintained as a hemizygote or homozygote and lacks any phenotype related to the transgenic allele. It has been well characterized and shown to be very efficient for performing liver-specific gene knockouts using Cre/loxP system. This line has been shown to be nearly 100% efficient in achieving liver-specific recombination when crossed with at least 5 different floxed alleles.
View cre expression characterization. | ||
| 006881 | B6.Cg-Tg(Aqp2-cre)1Dek/J | Repository- Live |
| Mice hemizygous for this AQP2-Cre transgene are viable and fertile. Transgenic cre activity, directed by the mouse aquaporin 2 promoter, is observed in kidney cells (collecting duct) and testes (sperm). When bred with mice containing a loxP-flanked sequence of interest, cre-mediated recombination will result in deletion of the flanked sequence. In such breedings, maternal inheritance of the transgene is recommended for kidney-specific recombinase activity as males express cre in sperm as well as kidney tissues. These AQP2-Cre mice may be used to generate conditional mutations in the renal collecting duct for studying nephrology, physiology, metabolism, or type II diabetes. In addition, cre expression in sperm may be useful in generating conditional mutations in multiple or all tissues in the resulting offspring. | ||
| 011104 | B6.Cg-Tg(Atoh1-cre)1Bfri/J | Repository- Live |
| In this strain, mouse Atoh1 (atonal homolog 1 (Drosophila)) regulatory sequences drives cre expression primarily in precursors of granule cell neurons of the cerebellum and dorsal hindbrain/spinal cord in the dp1 domain. When bred with mice containing sequences flanked by similarly oriented loxP sites, flanked sequences will be deleted in the Cre-expressing tissues of the offspring. | ||
| 016997 | B6.Cg-Tg(Axin2-rtTA2S*M2)7Cos/J | Repository- Live |
| These transgenic mice express an optimized reverse tetracycline-controlled transactivator, rtTAS*M2, or rtTA2S-M2, under the control of the mouse Axin2 expression cassette (which contains 2883 nucleotides upstream of exon 1, exon 1, intron 1 and part of exon 2). Transgene expression mimics the endogenous Axin2 expression pattern, and is detected in dorsal neural tube neuroepithelial cells, migrating neural crest cells, and branchial arch post-migratory neural crest cells. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. Mice that are hemizygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. T ..... For more information please see the full phenotype on the strain data sheet | ||
| 005317 | B6.Cg-Tg(BAT-lacZ)3Picc/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These "BAT-GAL" transgenic mice are a reporter strain that express beta-galactosidase in the presence of activated beta-catenin. The transgene expresses the lacZ gene under the control of a regulatory sequence consisting of seven consensus LEF/TCF-binding motifs upstream of the Xenopus siamois gene minimal promoter. Transgenic mice display beta-galactosidase activity beginning at embryonic day 6.0 in the posterior side of the proximal epiblast. Beta-galactosidase expression is detectable in the posterior primitive streak and node at gastrulation, and progresses to the paraxial mesoderm and notochord. Beta-galactosidase activity in developing and adult nervous tissue mimics the pattern of Wnt signaling. When bred to other mutant strains, this reporter strain may be useful for identifying mutations that affect the Wnt-signalling pa ..... For more information please see the full phenotype on the strain data sheet | ||
| 006051 | B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J | Repository- Live |
| Mice homozygous for this Actb-DsRed.T3 transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the red fluorescent protein variant DsRed.MST under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. All tissues of homozygotes fluoresce red. Mice hemizygous for the transgene express red fluorescent protein less intensely than homozygotes. Expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description as published results bec ..... | ||
| 008705 | B6.Cg-Tg(CAG-DsRed,-EGFP)5Gae/J | Repository- Live |
| Hemizygous IRG transgenic mice are viable and fertile, with widespread expression of a loxP-flanked optimized red fluorescent protein variant (DsRed-Express) directed to embryonic and adult tissues by the CAG promoter prior to exposure to Cre recombinase. When bred to cre-expressing mice, the resulting offspring have the DsRed-Express cassette deleted in the cre-expressing tissue(s), allowing expression of the enhanced green fluorescent protein (EGFP) cassette located just downstream. These IRG transgenic mice are a double-fluorescent, Cre-reporter strain; with widespread expression of red fluorescence prior to Cre recombinase exposure, and green fluorescence following cre-mediated recombination in a pattern determined by cre expression, and should provide a versatile tool for analyzing complex cellular relationships in a wide variety of tissues. For example, when using IRG transgenic mice along with Nestin-Cre mice (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 008112 | B6.Cg-Tg(CAG-Ub*G76V/GFP)2Dant/J | Repository- Live |
| Hemizygous transgenic mice are viable and fertile; they contain the green fluorescent protein (GFP) fused to a constitutively active degradation signal (UbG76V). Transgenic transcripts are detected in all tissues examined; however no GFP protein expression is detected due to the G76V substitution which leads to its ubiquitination and proteasomal degradation. Following administration of proteasome inhibitors, UbG76V accumulates and GFP-derived fluorescence is readily apparent, evidence of an impaired ubiquitin/proteasome system. This strain and UbG76V-GFP/1 (Stock No. 008111) have similar expression patterns, but this line (UbG76V-GFP/2) shows lower transgene expression and is not reported to display background fluorescence. These strains may be useful for monitoring ubiquitin/proteasome-dependent proteolysis in diverse disorders, and in efficacy trials for monitoring the effect of compou ..... For more information please see the full phenotype on the strain data sheet | ||
| 004682 | B6.Cg-Tg(CAG-cre/Esr1*)5Amc/J | Repository- Live |
| These transgenic mice have a tamoxifen-inducible cre-mediated recombination system driven by the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice. Homozygous mice are not viable or fertile. Heterozyg ..... For more information please see the full phenotype on the strain data sheet | ||
| 005884 | B6.Cg-Tg(CAG-mRFP1)1F1Hadj/J | Repository- Live |
| Mice homozygous for the transgene exhibit robust and widespread expression of monomeric red fluorescent protein-1 (mRFP1) in embryonic stem cells, embryos, and adults. Unlike tetrameric or dimeric fluorescent proteins, high levels of mRFP1 expression do not affect cell morphology, developmental potential, viability, and fertility of homozygous mice. Various optical imaging modalities can be used to visualize mRFP1 expressing cells in culture, in embryos and adult mice. Cells from transgenic mRFP1 mice, sampled along with green and cyan fluorescent cells from other mice, show clearly discernable fluorescence. This mouse may be useful in studies of mRFP1 cell lines, transplantation, embryo chimera experiments, and fluorescent imaging and technology development. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted tha ..... | ||
| 008520 | B6.Cg-Tg(CD2-cre)4Kio/J | Repository- Live |
| Mice hemizygous for this hCD2-iCre transgene are viable and fertile, with the human CD2 promoter and locus control region (LCR) directing expression of an optimized variant of Cre recombinase (iCre) to T cells and B cells (all committed B cell and T cell progenitors). Using crosses to a reporter strain, variegated germ line (testis) and a small monocyte-enriched population expression is also reported. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These hCD2-iCre transgenic mice may be useful for generating conditional mutations in T cells and B cells. Of note, this hCD2-iCre strain (Stock No. 008520) allows reliable deletion/gene targeting to be focused to T cells and B cells, whereas the Vav-iCre strain (Stock No. 008610) allows targeting throughout the entire hematopoietic compartment. IMPOR ..... | ||
| 009350 | B6.Cg-Tg(CDX2-cre)101Erf/J | Repository- Live |
| Mice hemizygous for the CDX2P9.5-NLSCre transgene are viable and fertile, with a 9.5 kb human caudal type homeo box 2 (CDX2) promoter/enhancer sequence directing expression of a nuclear-localized Cre recombinase predominantly to colonic epithelium during late gestation and in adult tissues. Specifically, Cre recombinase expression is observed in epithelium from the distal ileum and cecum, and throughout the colon from the crypt base to the luminal surface. Cre recombinase expression is also observed throughout the caudal region of the embryo during early development. When these transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. | ||
| 016882 | B6.Cg-Tg(CMV-CASP3)14Edge/J | Repository- Live |
| Mos-iCsp3 transgenic mice have a CMV promoter directing expression of a floxed-lacZ-STOP cassette followed by two tandem FK506-binding sites (Fvs) and a downstream human Caspase 3 (Casp3) gene. Hemizygous Mos-iCsp3 mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Caspase 3 is a member of the cysteine-aspartic acid protease family of enzymes which are integral to apoptosis pathways. Inactive until cleaved by an initiator enzyme, Caspase 3 is processed at conserved aspartic residues and is activated by the formation of dimers. In this transgenic strain, a STOP cassette is present, flanked by a loxH site and a loxP site, preventing inducible Caspase 3 expression and allowing for widespread lacZ staining. LacZ staining in founder line 14 is seen in the eye, kidney, pancreas, skin, thymus, and portions of the brain. The presence of both a loxH site and a loxP site causes reduc ..... For more information please see the full phenotype on the strain data sheet | ||
| 016908 | B6.Cg-Tg(CMV-CASP3)17Edge/J | Repository- Live |
| Mos-iCsp3 transgenic mice have a CMV promoter directing expression of a floxed-lacZ-STOP cassette followed by two tandem FK506-binding sites (Fvs) and a downstream human Caspase 3 (Casp3) gene. Hemizygous Mos-iCsp3 mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Caspase 3 is a member of the cysteine-aspartic acid protease family of enzymes which are integral to apoptosis pathways. Inactive until cleaved by an initiator enzyme, Caspase 3 is processed at conserved aspartic residues and is activated by the formation of dimers. In this transgenic strain, a STOP cassette is present, flanked by a loxH site and a loxP site, preventing inducible Caspase 3 expression and allowing for widespread lacZ staining. LacZ staining in founder line 17 is seen in the spinal cord, muscle, pancreas, skin, and portions of the brain and skull. The presence of both a loxH site and a loxP site cau ..... For more information please see the full phenotype on the strain data sheet | ||
| 005359 | B6.Cg-Tg(Camk2a-cre)T29-1Stl/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the Cre recombinase under the control of the mouse calcium/calmodulin-dependent protein kinase II alpha promoter. Cre recombinase expression is detected in the forebrain, specifically to the CA1 pyramidal cell layer in the hippocampus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination occurs in the pyramidal cell layer. | ||
| 007004 | B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ | Repository- Live |
| Transgenic mice expressing the tetracycline-controlled transactivator protein (tTA) under regulatory control of the forebrain-specific calcium-calmodulin-dependent kinase II (Camk2a) promoter are viable and fertile. When hemizygotes are mated to a second strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene can be blocked by administration of the tetracycline analog, doxycycline (dox). These mice are a "Tet-Off" tool that allow the inducible expression of genes in forebrain neurons, and may be useful in studying brain disorders such as Alzheimer's disease (when used in conjunction with Stock No. 005706, Stock No. 007049, Stock No. 007051, Stock No. 007052), Parkinson's ..... For more information please see the full phenotype on the strain data sheet | ||
| 005551 | B6.Cg-Tg(Cd4-TGFBR2)16Flv/J | Repository- Live |
| These transgenic mice express a dominant-negative form of the human transforming growth factor, beta receptor II (dnTGFBRII) under the direction of the mouse CD4 antigen promoter. Transgene expression is detected by RT-PCR analysis of thymus tissue, and is at a level sufficient to block TGF-beta signaling specifically in CD4+ and CD8+ T cells. At 3 to 4 months of age transgenic mice begin to display wasting and diarrhea. Histological examination reveals inflammatory bowel disease (IBD) and inflammatory infiltration of the large intestine, lungs, and liver. Less severe infiltration is observed in the stomach, duodenum, pancreas and kidney. The Donating Investigator indicates that the severity of and organs affected by the IBD is influenced by strain background. Western blot analysis detects circulating autoreactive antibodies. IgG immune deposits form in kidney glomeruli. There is a 3-fold increase in total cell numbers in peripheral lymphoid organs. T cells spontaneously differentiate ..... For more information please see the full phenotype on the strain data sheet | ||
| 012237 | B6.Cg-Tg(Cdh16-cre)91Igr/J | Repository- Live |
| Hemizygous and homozygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These Ksp1.3/Cre transgenic mice express Cre recombinase under the control of the mouse cadherin 16 (Cdh16 or Ksp-cadherin) promoter. Cre recombinase expression follows expression of the endogenous gene and is detected in the epithelial cells of developing nephrons, ureteric bud, mesonephric tubules, Wolffian duct, and Mullerian duct. In the adult mouse expression is limited to the renal tubules especially the collecting ducts, loops of Henle and distal tubules. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in the offspring. This mutant mouse strain may be useful in kidney-specific gene targeting and cell lineage studies. | ||
| 006137 | B6.Cg-Tg(Cdh5-cre)7Mlia/J | Repository- Live |
| Hemizygotes are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. In the differentiated endothelium transgene expression is observed as early as E7.5 and progresses to almost full penetrance by E14.5. In adult mice, uniform cre expression is observed in the endothelium of developing and quiescent vessels of all organs examined, as well as within a subset of hematopoietic cells. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome. These mice may be useful in studies of the cardiovascular system, including angiogenesis, and endothelial and hematopoietic cell lineages. | ||
| 014545 | B6.Cg-Tg(Chat-COP4*H134R/EYFP)5Gfng/J | Repository- Live |
| Mice hemizygous for the ChAT-mhChR2-YFP BAC transgene (or ChAT-ChR2-YFP BAC transgene) are viable and fertile, with expression of the mhChR2::YFP fusion protein directed to cholinergic neuronal populations by the mouse choline acetyltransferase (Chat or ChAT) promoter/enhancer regions on the BAC transgene. The mhChR2::YFP fusion protein is composed of a mammalian codon-optimized Chlamydomonas reinhardtii-derived channelrhodopsin-2 that was modified to harbor a gain-of-function H134R substitution (mhChR2; also called hChR2-H134R) fused in-frame with an enhanced yellow fluorescent protein (EYFP). The mhChR2 is designed to cause larger stationary photocurrents compared to ChR2. The bacterial opsins are retinal-binding proteins that provide light-dependent ion transport and sensory functions to a family of halophilic bacteria; and this mhChR2 functions as a blue light-driven cation channel that depolarizes the cell and causes action potentials. As such, illuminating mhChR2-ex ..... For more information please see the full phenotype on the strain data sheet | ||
| 014146 | B6.Cg-Tg(Ckm-DYSF)3Kcam/J | Repository- Live |
| Expression of human DYSF (dysferlin) cDNA is driven by the mouse Ckm (creatine kinase, muscle; MCK) promoter in this transgenic strain. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Expression of dysferlin in skeletal muscle is approximately 4-8 fold that of the endogenous protein level. There is no detectable muscle pathology up to one year of age. | ||
| 013134 | B6.Cg-Tg(Col1a1*2.3-GFP)1Rowe/J | Repository- Live |
| These transgenic mice express Enhanced Green Fluorescent Protein gene under the control of the 2.3 kb (from the upstream promoter sequence to 22300 bp) rat procollagen, type 1, alpha 1( Col1a1) promoter. In cultures of bone marrow stromal cells (MSC) isolated from mice 6-8 weeks of age, transgene expression is detected at day 14, before osteocalcin expression is detected, by Northern blot analysis. Fluorescence is detected in developing mineralized bone nodules in cultures of calvarial osteoblast cells isolated from neonates, and in osteocytes throughout adult cortical bone. No fluorescence is detected in dermal fibroblasts, bladder smooth muscle cells, peribronchial connective tissues, or periosteal fibroblasts from bone. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. | ||
| 016241 | B6.Cg-Tg(Col1a1-cre/ERT2)1Crm/J | Repository- Live |
| These transgenic mice express a tamoxifen inducible Cre recombinase driven by the 2.3-kb mouse Col1a1, collagen, type I, alpha 1, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in the osteoblasts of most bones and in odontoblasts of teeth in embryonic and postnatal mice. Inducible Cre recombinase activity is detected in the osteoblasts of the long bones of the limbs a ..... For more information please see the full phenotype on the strain data sheet | ||
| 016237 | B6.Cg-Tg(Col1a2-cre/ERT)7Cpd/J | Repository- Live |
| These transgenic mice express a tamoxifen-inducible Cre recombinase driven by the mouse Col1a2, collagen, type I, alpha 2, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions of the flanked sequence. Tamoxifen administration in double mutant mice, carrying this transgene and a beta-galactosidase reporter, induces Cre recombination in dermal and visceral fibroblasts. Transgenic embryos (aged 13.5 embryonic days) exhibit inducible Cre r ..... For more information please see the full phenotype on the strain data sheet | ||
| 006368 | B6.Cg-Tg(Cr2-cre)3Cgn/J | Repository- Live |
| Mice homozygous for this "CD21-cre3a" transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse complement receptor 2 (CR2) promoter. Cre recombinase expression is detected specifically in mature transitional B cells. When CD21-cre3a mice were crossed to a lacZ-expressing Cre reporter strain, Cre recombinase activity is detected in 65-70% of mature B cells (as determined by FACS analysis of bone marrow cells). When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in mature B lymphocytes and follicular dendritic cells. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study B lymphocyte development. | ||
| 008538 | B6.Cg-Tg(Cspg4-cre/Esr1*)BAkik/J | Repository- Live |
| Mice hemizygous for the NG2CreERTM BAC transgene are viable and fertile, with the mouse NG2 (Cspg4) promoter/enhancer directing expression of a tamoxifen-inducible Cre recombinase (CreERTM). This CreERTM fusion protein is estrogen insensitive, and is only active when it binds the estrogen analog 4-hydroxytamoxifen (OHT) or tamoxifen.
When these NG2CreERTM BAC transgenic mice are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. No background cre activity is reported in the absence of tamoxifen. Following tamoxifen administration, the majority of Cre recombinase activity is reported in NG2-expressing glia (polydendrocytes, oligodendrocyte progenitor cells). The donating investigator specifically reports that tamoxifen-induced Cre recombinase activity is observed throu ..... For more information please see the full phenotype on the strain data sheet | ||
| 016204 | B6.Cg-Tg(Drd1a-tdTomato)6Calak/J | Repository- Live |
| These mutant mice harbor a transgenic BAC containing the mouse dopamine receptor D1A (Drd1a) promoter directing expression of a modified DsRed fluorescent protein, tdTomato. These Drd1a-tdTomato mice (founder line 6) express tdTomato in striatonigral neurons in a more accurate and specific manner than seen in previous founder lines. Hemizygous mice are viable, fertile and normal in size. These mice may be useful for specific visualization of striatonigral projection neurons and for studying dopamine-sensitive behaviors. | ||
| 005069 | B6.Cg-Tg(Fabp4-cre)1Rev/J | Repository- Live |
| These transgenic mice express Cre recombinase under the control of the mouse Fabp4, fatty acid binding protein 4, adipocyte, promoter. Cre recombinase expression is detected in brown and white gonadal and subcutaneous adipose tissue. No expression is detected in skeletal muscle. When crossed with a strain containing a loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene specifically in adipose tissue. Mice that are homozygous for the targeted mutation are viable. This strain represents an effective tool for generating tissue-specific targeted mutants.
View cre expression characterization. | ||
| 004633 | B6.Cg-Tg(GFAP-APOE_i3)37Hol Apoetm1Unc/J | Repository- Live |
| These transgenic mice express the human apolipoprotein E3 isoform (APOE3) under the direction of the human glial fibrillary acidic protein (GFAP) promoter and do not express endogenous mouse apolipoprotein E (APOE). The transgenic isoform expression pattern follows the endogenous mouse APOE and GFAP expression patterns in the brain. Human APOE3 is immunodetectable in glia and neuropil in developing and adult mutant mice. Cultured astrocytes from transgenic mice secrete APOE3 in lipoproteins that are similar in size to high-density (HDL) plasma lipoproteins. Detergent-soluble APOE3 protein levels in hemizygous mice forebrain tissue and in adult human cortex tissue are similar. Mice that are hemizygous or homozygous for the transgenic insert and homozygous for the targeted allele are viable, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain represents a model that may be useful in studies examining the function of APOE3 in t ..... For more information please see the full phenotype on the strain data sheet | ||
| 004631 | B6.Cg-Tg(GFAP-APOE_i4)1Hol Apoetm1Unc/J | Repository- Live |
| These transgenic mice express the human apolipoprotein E4 isoform (APOE4) under the direction of the human glial fibrillary acidic protein (GFAP) promoter and do not express endogenous mouse apolipoprotein E (APOE). The transgenic isoform expression pattern follows the endogenous mouse APOE and GFAP expression patterns in the brain. Human APOE4 is immunodetectable in glia and neuropil in developing and adult mutant mice. Cultured astrocytes from transgenic mice secrete APOE4 in lipoproteins that are similar in size to high-density (HDL) plasma lipoproteins. Detergent-soluble APOE4 protein levels in hemizygous mice forebrain tissue and in adult human cortex tissue are similar. Mice that are hemizygous or homozygous for the transgenic insert and homozygous for the targeted allele are viable, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain represents a model that may be useful in studies examining the function of APOE4 in t ..... For more information please see the full phenotype on the strain data sheet | ||
| 014095 | B6.Cg-Tg(GFAP-Ccl2)JE95Rmra/J | Repository- Live |
| Mice homozygous for the huGFAP-MCP-1 transgene are viable and fertile, with expression of mouse monocyte chemoattractant protein-1 (MCP-1 or Ccl2) directed primarily to astrocytes of the CNS by the human glial fibrillary acidic protein (GFAP) promoter. Transgene-directed mRNA and protein expression is observed in CNS lysates and astrocyte cultures, as well as in peripheral nerve (such as sciatic; because GFAP is expressed by nonmyelinating Schwann cells). Mice derived from the high-expressing founder line 95 (also called huGFAP-MCP-1hi tg+, huGFAP-CCL2hi tg+, or JE-95 mice) overexpress CCL2 in an astroglial activation-dependent manner. Levels of CCL2 expression in the CNS of huGFAP-CCL2hi tg+ mice were comparable with those observed in CNS tissues from mice with experimental autoimmune encephalomyelitis (EAE). With chronic overexpression of CCL2, aged huGFAP-CCL2hi tg+ mice develop D ..... For more information please see the full phenotype on the strain data sheet | ||
| 014098 | B6.Cg-Tg(GFAP-rtTA*M2)1Rmra/J | Repository- Live |
| The GFAP-rtTA*M2 transgene contains the human glial fibrillary acidic protein, GFAP, promoter directing expression of an optimized form of the reverse tetracycline-controlled transactivator (rtTA*M2) protein. Homozygous mice are viable, fertile, and normal in size. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the gene of interest may be regulated by the tetracycline analog, doxycycline (dox). In the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. This strain provides a Tet-On tool that allows the inducible expression of genes in astrocytes of the central nervous system. | ||
| 005964 | B6.Cg-Tg(GFAP-tTA)110Pop/J | Repository- Live |
| Mice hemizygous for the transgene are viable fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice express a tetracycline-controlled transactivator protein (tTA) driven by the human glial fibrillary acidic protein (GFAP) promoter. When these mice are mated to a second transgenic strain that carries a target gene under the regulation of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in astrocytes in the bitransgenic offspring can be induced by withdrawal of the tetracycline analog, doxycycline. Doxycycline may be administered in the animals' water supply. This strain represents an effective tool for generating bitrangenic animals that may be useful to study inducible gene expression in the astrocytes of the central nervous system. | ||
| 007673 | B6.Cg-Tg(Gad1-EGFP)3Gfng/J | Repository- Live |
| Mice hemizygous for this GAD67-GFP transgene are viable and fertile. In the hippocampus, GAD67-GFP (line 3) mice selectively express enhanced green fluorescent protein (EGFP) in newborn dentate granule cells. At two weeks of age, EGFP is expressed in large numbers of dentate granule cells, and the number of EGFP-positive cells diminishes with age in a pattern consistent with the postnatal development of dentate granule cells/age-related reduction in adult neurogenesis in the dentate gyrus. The EGFP expression in newborn dentate granule cells begins within the first week of cell division and disappears as newborn neurons mature (about 4 weeks postmitotic), and EGFP positive dentate granule cells also express the newborn neuron markers PSA-NCAM and doublecortin. The bright labeling of newborn neurons with EGFP makes it possible to visualize the details of dendrites, which reach the outer edge of the molecular layer, and
their axon terminals (mossy fiber boutons) in the CA3 region. In ad ..... For more information please see the full phenotype on the strain data sheet | ||
| 005698 | B6.Cg-Tg(Gfap-TK)7.1Mvs/J | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, normal in size, and do not display any behavioral abnormalities. Transgenic males are infertile. Proliferating cells that express the herpes simplex virus thymidine kinase (HSV-TK) transgene will metabolize ganciclovir (GCV) to toxic nucleotide analogues and undergo cell death. Transgene-derived HSV-TK is present exclusively in cells expressing endogenous Gfap. This coexpression occurs in brain astrocytes and adult neural stem cells, enteric glia, hepatic stellate cells, and unknown cells in heart, lung, kidney, adrenal, and spleen. Chronic GCV treatment for 21 days depletes GFAP-positive adult neural stem cells from forebrain proliferative zones. GCV treatment eliminated growth of primary multipotent neurospheres cultured from the germinal zones of postnatal and adult, but not early embryonic, transgenic mice. Notably, the same treatment prevented growth of secondary multipotent neurospheres from all three developmental stag ..... For more information please see the full phenotype on the strain data sheet | ||
| 012886 | B6.Cg-Tg(Gfap-cre)73.12Mvs/J | Repository- Live |
| Mice hemizygous for the Gfap-cre transgene are viable and fertile, with a mouse glial fibrillary acidic protein (mGfap) promoter sequence directing expression of a Cre-recombinase. Specifically, Cre recombinase activity (as defined by expression of loxP-STOP flanked reporter gene) is targeted to most astrocytes throughout healthy brain and spinal cord tissues and to essentially all astrocytes following Central Nervous System (CNS) injury. Cre recombinase activity is observed in essentially all adult neural stem cells and their progeny in the hippocampal dentate gyrus and subventricular zone. In addition, some hippocampal pyramidal neurons and cerebellar granule neurons (less than 5%) and scattered neurons in the midbrain (less than 0.5%) also exhibit activity due to radial cell progenitors that begin to express Gfap at later developmental stages in post-natal mice when the last-born neurons are generated. Mice hemizygous for the Gfap-cre transgene are viable and fertile.
..... For more information please see the full phenotype on the strain data sheet | ||
| 012887 | B6.Cg-Tg(Gfap-cre)77.6Mvs/J | Repository- Live |
| Mice hemizygous for the Gfap-cre transgene are viable and fertile, with a mouse glial fibrillary acidic protein (mGfap) promoter sequence directing expression of a Cre-recombinase. Specifically, Cre recombinase activity (as defined by expression of loxP-STOP flanked reporter gene) is targeted to most astrocytes throughout the healthy brain and spinal cord and to essentially all astrocytes after Central Nervous System (CNS) injury. Cre recombinase activity is also targeted to a subpopulation of the adult stems in the subventricular zone. In contrast to GFAP-Cre line 73.12 , there is no targeting of postnatal or adult neural stem cells or their progeny in the hippocampus or other brain regions in GFAP-Cre line 77.6, rendering these mice particularly useful for selective targeting of astrocytes. When these transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombina ..... For more information please see the full phenotype on the strain data sheet | ||
| 007897 | B6.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J | Repository- Live |
| Mice hemizygous for this ROSA26-EGFP BAC transgene are viable and fertile, with widespread EGFP expression in all organs and tissues analyzed. In addition, stable and uniform EGFP expression is observed in differentiated progeny of transplanted hematopoietic stem cells (HSC) isolated from ROSA26-EGFP mice. These ROSA26-EGFP BAC transgenic mice may be useful for fluorescent labeling of cells and tissues both in vivo and in vitro, are suitable for use in histological preparations, and are appropriate as a source of fluorescently labeled donor cell populations (such as tracking hematopoietic cell fate in transplant recipients).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as publis ..... | ||
| 006069 | B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express an "H2B-EGFP" fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Green Fluorescent Protein gene [EGFP, BD Biosciences]) under the control of the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer. Nucleosomes and chromatin in all cells fluoresce. Fluorescence is detectable during all phases of mitosis. The donating investigator reports occasional silencing of the transgene when transmitted through the female germline. This mutant mouse strain may be useful for in vivo subcellular high-resolution and 3-dimensional studies of dividing cells.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from ..... | ||
| 004191 | B6.Cg-Tg(HLA-A/H2-D)2Enge/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express an interspecies hybrid class I MHC gene, AAD, which contains the alpha-1 and alpha-2 domains of the human HLA-A2.1 gene and the alpha-3 transmembrane and cytoplasmic domains of the mouse H-2Dd gene, under the direction of the human HLA-A2.1 promoter. Immunodetection of the HLA-A2.1 recombinant transgene established that expression was at equivalent levels to endogenous mouse class I molecules. The mouse alpha-3 domain expression enhances the immune response in this system. Compared to unmodified HLA-A2.1, the chimeric HLA-A2.1/H2-Dd MHC Class I molecule mediates efficient positive selection of mouse T cells to provide a more complete T cell repertoire capable of recognizing peptides presented by HLA-A2.1 Class I molecules. The peptide epitopes presented and recognized by mouse T cells in the ..... For more information please see the full phenotype on the strain data sheet | ||
| 005029 | B6.Cg-Tg(Hlxb9-GFP)1Tmj/J | Repository- Live |
| These transgenic mice express Green Fluorescent Protein (GFP) under the direction of the mouse Mnx1 (also called Hlxb9) promoter. Mice homozygous for the transgenic insert are viable, fertile, do not display any gross behavioral abnormalities, but are smaller in size than wildtype littermates. Homozygous pups born to homozygous females have a high mortality rate. Transgenic mice display distinct expression of GFP in dendrites, axons and soma of spinal motor neurons, allowing identification, isolation and purification of spinal motor neurons by FACS. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice. This mutant mouse strain represents a model that may be useful for purification and in vivo tracking of spinal motor neurons. | ||
| 002728 | B6.Cg-Tg(IghMyc)22Bri/J | Repository- Live |
| Expression of the mouse Myc transgene is restricted to the B cell lineage. Hemizygotes show increased pre-B cells in the bone marrow throughout life and a transient increase in large pre-B cells in the blood at 3-4 weeks of age. Spontaneous pre-B and B cell lymphomas reach an incidence of 50% at 15-20 weeks in hemizygous progeny of a wildtype female mated with a hemizygous male. The transgene synergizes with an TgN(BCL2)22Wehi transgene (Stock No. 02319) to produce primitive lymphoid tumors within 5 weeks of birth, and with an Emu-v-abl transgene to produce plasmacytomas by 8 weeks. | ||
| 006864 | B6.Cg-Tg(Ins1-EGFP)1Hara/J | Repository- Live |
| Mice hemizygous for this "MIP-GFP" transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the control of the mouse insulin 1 promoter. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adulthood. The fluorescence expression pattern is similar to the patterns seen in other stocks (see Stock No. 006784 and Stock No. 006866). MIP-GFP transgenic mice exhibit normal glucose tolerance and pancreatic insulin levels. The human growth hormone (hGH) sequence in the transgenic insert enhances expression of the EGFP, but hGH is not expressed. This mutant mouse strain may be useful in studies of diabetes and pancreatic beta islet ..... For more information please see the full phenotype on the strain data sheet | ||
| 003573 | B6.Cg-Tg(Ins2-cre)25Mgn/J | Repository- Live |
| This strain carries the "RIP-Cre" transgene construct (containing a 668 bp fragment of the rat insulin II promoter, Cre recombinase with a nuclear localization sequence, and a 2.1 kb fragment from the human growth hormone gene). Hemizygous mice carrying this transgene are phenotypically normal and overexpresss cre specifically in pancreatic beta cells. This transgene strain is used in combination with mice carrying floxed targeted mutations to create various pancreatic beta cell-specific gene knockouts using the "Cre-lox" system. Results from several different laboratories have shown that this transgenic strain is at least 85% efficient in achieving pancreatic beta cell-specific recombination. It should also be noted that the transgene in this line has been found to be expressed at a low level in the hypothalamus. In some cases this has resulted in a phenotype due to deletion of the floxed allele in this region of the brain. It has also been shown that these transgenic mice may ..... For more information please see the full phenotype on the strain data sheet | ||
| 008378 | B6.Cg-Tg(Itgax-TGFBR2)1Flv/J | Repository- Live |
| These CD11c-dnTGFβRII transgenic mice express a dominant negative form of the human transforming growth factor, beta receptor II gene under the control of a CD11c (Itgax) promoter. This form of the receptor blocks transforming growth factor, beta (Tgfb) signalling when its expression is sufficiently high to interfere with the assembly of a functional signaling complex consisting of transforming growth factor beta and type II and type I transforming growth factor beta receptors. Expression of the transgene is detected by RT-PCR in CD11c-expressing cells, including natural killer (NK) cell and lymphoid/myeloid dendritic cell (DC) subsets. Expression is not detected in natural killer T cells (NKT) cells, T cells or B cells isolated from transgenic mice. Transgenic mice show a selective dysregulation of NK cell homeostasis due to the blockage of transforming growth factor, beta (Tgfb) signalling. Large numbers of NK cells capable of producing considerable levels ..... For more information please see the full phenotype on the strain data sheet | ||
| 008829 | B6.Cg-Tg(Itgax-Venus)1Mnz/J | Repository- Live |
| This transgenic strain expresses yellow fluorescent protein (YFP) under the transcriptional control of the mouse integrin alpha X (Cd11c) promoter which is exclusively expressed in dendritic cells of the immune system. | ||
| 008068 | B6.Cg-Tg(Itgax-cre)1-1Reiz/J | Repository- Live |
| Mice hemizygous for the Cd11c-cre transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse integrin alpha X (Cd11c) promoter. Cre-mediated recombination is detected in more than 95% of conventional CD11chigh dendritic cells both from lymphoid organs and from non-lymphoid tissues such as lung and epidermis, and in 50-80% of plasmacytoid dendritic cells. The dendritic cell compartment of transgenic mice is normal. Relatively low amounts of recombination are detected in lymphocytes (<10%), NK cells (12%), and myeloid cells (<1%). No increase of recombination frequency was observed in CD11clow- activated T cells. These Cd11c-cre transgenic mice (as well as CD11c-Cre-GFP transgenic mice (see Stock No. 007567)) are an effective tool for generating tissue-specific targeted mutant ..... For more information please see the full phenotype on the strain data sheet | ||
| 008781 | B6.Cg-Tg(Kap-cre)29066/2Sig/J | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase (iCre, improved cre) under the control of the mouse kidney androgen regulated protein (Kap). Cre recombinase expression is detected in the proximal tubule cells of the renal cortex in male mice. Female mice do not express the transgene unless treated with androgen (testosterone). When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the proximal tubule cells of the kidney. The Donating Investigator reports that for transgenic mice on the C57BL/6J background (both male and female), the transgene needs to be induced with exogenous androgen. The Donating Investigator recommends using a testosterone pellet implanted subcutaneously, which results in a modest level of transgene induction 10 d ..... For more information please see the full phenotype on the strain data sheet | ||
| 005244 | B6.Cg-Tg(Krt1-15-EGFP)2Cot/J | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the direction of the mouse keratin complex 1, acidic, gene 15 promoter. The high levels of specific transgene expression observed in 'bulge cells' (hair follicle stem cells) allow these cells to be isolated with FACS techniques. This mutant mouse strain may be useful in studies of epithelial stem cells. | ||
| 013738 | B6.Cg-Tg(LCKprBCL2L1)12Sjk/J | Repository- Live |
| These lckpr-bcl-XL transgenic mice express a human B-cell leukemia/lymphoma 2 like, 1 (BCL2L1 or BCL-XL) cDNA sequence, under the control of a mouse lymphocyte protein tyrosine kinase proximal promoter (Lckpr). Mice that are heterozygous for this transgene are viable, fertile, and normal in size. Lckpr drives BCL-XL expression within all thymocyte subsets and protects thymocytes from a variety of apoptotic stimuli, including γ irradiation, glucocorticoids, and anti-CD3 treatment. This BCL-XL overexpression alters thymocyte maturation, resulting in an increase in CD3int/hi and a decrease in CD3lo thymocytes, along with an increase in intermediate CD4+8lo and CD4lo8+ thymocytes. They also exhibit an increase in single positive CD8 T cells in the spleen. Overexpression of BCL-XL causes down-regulation of B-cell leukemia/lymphoma 2 (BCL-2) an ..... For more information please see the full phenotype on the strain data sheet | ||
| 012837 | B6.Cg-Tg(Lck-cre)3779Nik/J | Repository- Live |
| Mice hemizygous for the Lck-cre transgene are viable, fertile, and normal in size. These mice contain a Cre-recombinase gene driven by the distal promoter of the lymphocyte protein tyrosine kinase (Lck) gene. Cre recombinase expression is delayed, and is only observed in T cells after T cell receptor α (Tcra) locus rearrangement and after the cells have reached the TCRhi stage of thymocyte development. When these transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination results in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. These mice are useful for studying the consequences of mutations induced after positive selection in the thymus. | ||
| 003802 | B6.Cg-Tg(Lck-cre)548Jxm/J | Repository- Live |
| Homozygous mice are viable and have no major defects. This strain expresses Cre recombinase in thymocytes. | ||
| 012467 | B6.Cg-Tg(Lrrk2*G2019S)2Yue/J | Repository- Live |
| Mice hemizygous for the BAC FLAG-Lrrk2-G2019S transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice express a mutant form of mouse leucine-rich repeat kinase 2 (Lrrk2-G2019S) associated with autosomal dominant, late-onset Parkinson's disease directed by the endogenous Lrrk2 promoter/enhancer regions on the BAC transgene. These BAC FLAG-Lrrk2-G2019S mice "overexpress" the mouse LRRK2-G2019S mutant protein in cerebral cortex, striatum, substantia nigra, internal capsule, and hippocampus at an approximately 6-8 fold greater level than endogenous mouse Lrrk2. These mice have reduced striatal dopamine content and may be useful for studying Parkinson's disease pathogenesis and neurodegeneration elicited by the dominant toxic effects of mutant LRRK2-G2019S expression. | ||
| 012643 | B6.Cg-Tg(Ly6a-EGFP)G5Dzk/J | Repository- Live |
| Ly6a-GFP transgenic mice have an enhanced green fluorescent protein (EGFP) under the control of murine lymphocyte antigen 6 complex, locus A (Ly6a) promoter. Hemizygous Ly6a-GFP mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Ly6a promoter directs the expression of GFP in all functional repopulating adult hematopoietic stem cells (HSCs) in the adult bone marrow, and several other hematopoietic cell types. The GFP transgene expression pattern generally corresponds to that of Sca-1, a glycoprotein-I-linked cell-surface glycoprotein used routinely as a marker of adult hematopoietic stem cells. These mice may be useful for the visualizing and sorting of hematopoietic stem cells. | ||
| 003556 | B6.Cg-Tg(Mx1-cre)1Cgn/J | Repository- Live |
| The Cre recombinase is under the control of the Mx1 promoter. This promoter is silent in healthy mice, but can be induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA (such as poly I:C). When combined with a mutant carrying a gene that has been flanked by loxP recognition sites, the expression of Cre recombinase causes the flanked gene to be removed. This provides researchers with the capability to induce the "knockout" at any time during development. There was ~1% background recombination seen in mice not treated with interferon. The percent deletion of the targeted gene varied depending on tissue type, presumably due to the amount of interferon-responsive cells present or to the availability of interferon in each organ.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an ..... | ||
| 007742 | B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J | Repository- Live |
| Mice hemizygous for the smMHC/Cre/eGFP transgene (smMHCCre/eGFP) are viable and fertile, with the smooth muscle myosin heavy chain (smMHC or Myh11) promoter directing bicistronic Cre and EGFP protein expression to smooth muscle cells during development as well as in the adult mouse. Hemizygotes from founder line SMCG2 (SM2Cre/GFP) display intense EGFP fluorescence restricted to vascular and nonvascular smooth muscle, with strong concordance between cre expression and EGFP fluorescence (verifying the use of fluorescence as a marker for conditional gene recombination). When bred with mice containing a loxP-flanked sequence of interest, the resulting offspring can have Cre-mediated recombination of the flanked sequence in smooth muscle. Homozygotes are viable and fertile, with smaller litter sizes and a higher incidence of perinatal mortality. These smMHC/Cre/eGFP transgenic mice may be useful in studies utilizing "Cre-lox" technology or fluoresc ..... For more information please see the full phenotype on the strain data sheet | ||
| 008205 | B6.Cg-Tg(NPHS2-cre)295Lbh/J | Repository- Live |
| Podocin-Cre mice (mice harboring the p2.5P-Cre transgene) are viable and fertile, with expression of Cre recombinase directed to podocytes within kidney glomeruli by the human podocin (NPHS2) promoter/enhancer region. Cre-recombinase activity is reported in podocytes during late capillary loop stage of glomerular development and persists in podocytes of mature glomeruli, with no evidence for cre expression detected in other tissues examined. Embryonic Cre-recombinase activity is also reported as early as 8.5 dpc. When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the sequence. These Podocin-Cre mice (mice harboring the p2.5P-Cre transgene) may be useful in generating conditional knockouts for studying the role of podocyte nephrobiology in renal disorders. | ||
| 003771 | B6.Cg-Tg(Nes-cre)1Kln/J | Repository- Live |
| These transgenic mice express Cre recombinase under the control of the rat nestin promoter and enhancer. Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Initial studies utilizing a reporter strain carrying a beta galactosidase transgene whose expression is dependent on Cre-mediated recombination indicate that cre is primarily expressed in the central and peripheral nervous system with a few isolated kidney and heart cells also expressing activity. The donating investigator indicates that Cre recombinase activity is present in nervous tissue by embryonic day 11. The transgene insertion location is on Chromosome 12, as determined by FISH analysis, view pdf.
View cre expression characterization. | ||
| 004662 | B6.Cg-Tg(PDGFB-APP)5Lms/J | Repository- Live |
| These transgenic mice express a wildtype human amyloid protein precursor (APP) under the control of the human platelet-derived growth factor beta polypeptide (PDGFB) promoter. PCR primer modification was used to alter the sequence of the APPInd mutation to the wildtype sequence in this transgene. Hemizygotes express immunodetectable transgene product in cerebral neurons, with the highest level of expression occurring in the neocortex and hippocampus. Enzyme-linked immunosorbent assay (ELISA) analysis of neocortical and hippocampal tissue reveals approximate total amyloid beta peptides levels and 42 amino acid length amyloid beta peptides levels that are lower than levels found in the APP SwInd mutant line. No amyloid plaques are detected by immunohistochemistry at 8-10 months of age or at 24 months of age. Mutants display age dependent decrease in density of synaptophysin-immunoreactive presynaptic terminals indicative of neurodegeneration. This strain serves as the control for Stock N ..... For more information please see the full phenotype on the strain data sheet | ||
| 013545 | B6.Cg-Tg(PER2*S662D)405Ljp/J | Repository- Live |
| These transgenic mice express a S662D (serine -> aspartic acid) amino acid mutation in the human PER2 (period homolog 2 (Drosophila)) gene. This mutation mimics a constitutively phosphorylated serine which may affect protein stability. These animals demonstrate a lengthened circadian period (24.3 hours in hemizygotes and 24.7 hours in homozygotes versus 23.7 hours in wildtype controls). This strain may be useful in studies of familial advanced sleep phase syndrome (FASPS) and PER2 regulation of circadian rhythms. | ||
| 013546 | B6.Cg-Tg(PER2*S662G)864Ljp/J | Repository- Live |
| These transgenic mice express a S662G (serine -> glycine) amino acid mutation in the human PER2 (period homolog 2 (Drosophila)) gene associated with familial advanced sleep phase syndrome (FASPS). The phenotype of these mice is reported to faithfully recapitulate the condition found in humans. Animals from both founder lines 864 and 867 demonstrate a short circadian period (21.6 hours in hemizygotes and 20.7 hours in homozygotes versus 23.7 hours in wildtype controls) and an advanced sleep phase. This strain may be useful in studies of familial advanced sleep phase syndrome (FASPS) and PER2 regulation of circadian rhythms. | ||
| 010536 | B6.Cg-Tg(Pcp2-cre)3555Jdhu/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse Purkinje cell protein (Pcp2). Cre recombinase expression is detected in Purkinje cells of the cerebellar folia and retinal bipolar cells. Expression was not found in spinal cord, heart, liver, kidney or cornea. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the offspring. This mutant mouse strain may be useful in studies of the nervous system, particularly Purkinje cells and retinal bipolar cells. | ||
| 005975 | B6.Cg-Tg(Plp1-cre/ERT)3Pop/J | Repository- Live |
| These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse Plp1, proteolipid protein (myelin) 1 promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration allows for ablation of predetermined genes in oligodendrocytes and Schwann cells, and will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice. M ..... For more information please see the full phenotype on the strain data sheet | ||
| 008827 | B6.Cg-Tg(Prdm1-cre)1Masu/J | Repository- Live |
| These transgenic mice express Cre recombinase under the control of the mouse Prdm1 (PR domain containing 1, with ZNF domain; Blimp1) promoter. Cre-mediated recombination is detected in 55-76% of primordial germ cells when this strain is crossed with Gt(ROSA)26-GFP reporter mice. Expression is also seen in plasma cells. These mice may be useful for generating tissue-specific targeted mutants for studies of development and germ cell fate. | ||
| 008596 | B6.Cg-Tg(Prnp-Abca1)EHol/J | Repository- Live |
| These transgenic mice express the mouse Abca1 (ATP-binding cassette, sub-family A (ABC1), member 1) gene under the control of the mouse Prnp (prion protein) promoter. These founder line E mice have a 6-fold increase in expression of ABCA1 in the cortex over wild-type levels. Trangene expression is high in total brain tissue, kidney, testis and muscle as detected by Western blot analysis. Transgenic mice exhibit reduced apoE levels: 40% of wild-type levels in the hippocampus, approximately half of wild-type levels in cerebrospinal fluid (CSF). The apoE protein that is overexpressed has altered biochemical properties and is not as soluble as that found in controls. Lipoprotein particles from the CSF that contain apoE protein are larger in size than wild-type, indicating that the transgenic apoE particles are more lipidated.
Male transgenic mice have atropied testes, defective spermatogenesis and are infertile. The strain can be maintained by mating hemizygous femal ..... | ||
| 010700 | B6.Cg-Tg(Prnp-TARDBP*A315T)95Balo/J | Repository- Live |
| Mice hemizygous for this Prp-TDP43A315T transgene are viable, fertile, and express a mutant human TAR DNA binding protein (TARDBP or TDP-43) cDNA harboring an N-terminal Flag tag and an A315T amino acid substitution that is associated with familial Amyotrophic Lateral Sclerosis (ALS). Expression is directed throughout the nervous system by mouse prion protein (PrP or Prnp) promoter/enhancer regions.
Hemizygous mice were originally published on a mixed C57BL/6;CBA genetic background and develop a progressive gait disorder around 3-4 months of age with death around 5 months of age. For hemizygous mice on a mixed C57BL/6;CBA genetic background, the donating investigator reports that, on average, males die almost one month earlier than females. Due to continued backcrossing to C57BL/6J at The Jackson Laboratory Repository, this strain is now fully congenic on a C57BL/6J background. Survival differences between male and female hemizygous mice are still obser ..... | ||
| 005584 | B6.Cg-Tg(Prrx1-cre)1Cjt/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the paired related homeobox 1 promoter. Cre recombinase expression closely patterns endogenous gene expression and is detectable by embryonic day 9.5. Some recombination occurs in the female germline. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in early limb bud mesenchyme. This strain represents an effective tool for generating tissue specific-targeted mutants useful in studies of limb bud development and patterning. | ||
| 007902 | B6.Cg-Tg(RP23-268L19-EGFP)2Mik/J | Repository- Live |
| Mice hemizygous for the ChAT(BAC)-eGFP (ChATBAC-eGFP) transgene are viable and fertile, with the endogenous choline acetyltransferase (ChAT) transcriptional regulatory elements (cholinergic gene locus) directing enhanced green fluorescent protein (EGFP) protein expression during development as well as in the adult mouse. As such, EGFP is expressed in central and peripheral cholinergic neurons, including cell bodies and processes of the somatic motor, somatic sensory, and parasympathetic nervous system in gastrointestinal, respiratory, urogenital, cardiovascular, and other peripheral organ systems and neuromuscular junctions. These ChAT(BAC)-eGFP transgenic mice allow fluorescent visualization of cholinergic elements of the central and peripheral nervous system and may be useful for studying cholinergic neurotransmission and neuromuscular coupling. | ||
| 012893 | B6.Cg-Tg(S100a4-EGFP)M1Egn/YunkJ | Repository- Live |
| These transgenic mice express GFP under the control of the mouse S100a4, S100 calcium binding protein A4, promoter. All tissue fibroblasts in adult hemizygous mice fluoresce. Woods light examination of the eyes of adult transgenic mice reveal green fluorescence of the fibroblasts of the cornea. Green fluorescing fibroblasts are detected in kidney, liver, lung, heart and in interstitial organ spaces. Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. | ||
| 012902 | B6.Cg-Tg(S100a4-TK)M31Egn/YunkJ | Repository- Live |
| These transgenic mice express a truncated herpesvirus thymidine kinase (TK) gene under the control of the S100a4, S100 calcium binding protein A4, (also called FSP1, fibroblast-specific protein-1), promoter. The truncated TK minigene lacks the cryptic promoter between the first and second ATG of the coding sequence which is active in testes and confers male sterility. The transgene was found to be expressed in the interstitium of the kidney and transgene mRNA is expressed at higher levels in fibroblasts than in renal tubular epithelium.
Dividing (proliferating) fibroblasts are selectively ablated in transgenic mice when treated with gancyclovir. Cultured primary fibroblasts from transgenic mice exhibit significant depletion 5 days after 10 mM gancyclovir treatment. Cultured renal tubular epithelium cells are not gancyclovir sensitive. Transgenic mice exhibit reduced fibrosis in experimentally induced renal obstruction. Founder line M31 contains several copies of the ..... For more information please see the full phenotype on the strain data sheet | ||
| 005999 | B6.Cg-Tg(SBE/TK-luc)7Twc/J | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express luciferase in response to activation of the Smad2/3-dependent signaling pathway. Cultured primary astrocytes isolated from transgenic mice exhibited luciferase activity when stimulated with TGF-beta. Higher treatment levels of activin and nodal elicited similar luciferase activity. Lipopolysaccharide (LPS) challenge results in strong bioluminescence emissions from the intestinal region and brain. Mechanical injury to the neocortex results in an increase of bioluminescence in 2 hours, which peaks at 4 hours and returns to baseline approximately 48 hours after the injury. Biochemical assays for luciferase activity correlated with noninvasive bioluminescence imaging analysis. The strain was backcrossed to the albino C57BL/6J-Tyrc-2J/J strain for 2 generations to facilitate bioluminescence imaging. ..... For more information please see the full phenotype on the strain data sheet | ||
| 006235 | B6.Cg-Tg(SFTPC-rtTA)5Jaw/J | Repository- Live |
| Mice that are hemizygous for this transgenic insert are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human SFTPC, surfactant, pulmonary-associated protein C, promoter. In situ hybridization detects rtTA gene product (mRNA) in lung peripheral epithelial cells from adult mice and 15 postconception day aged embryos from doxycycline treated dams. Induction of transgene expression is detected as early as postconception day 12.5 when the pregnant female is treated with doxycycline. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is ..... For more information please see the full phenotype on the strain data sheet | ||
| 008629 | B6.Cg-Tg(SMN2)11Tro Smn1tm1Msd/J | Repository- Live |
| Mice that are homozygous for both the Smn1tm1Msd allele and SMN2 (survival of motor neuron 2, centromeric, human) transgene, founder line 11, exhibit a very severe phenotype with survival ranging from hours up to 7 days after birth. Mice that die at or shortly after birth are slightly smaller than normal littermates. Mice that survive for several days are indistinguishable from normal littermates in the first 48 hours, after which they exhibit diminished weight gain. Mice hemizygous for the transgene and homozygous for the targeted mutation display an embryonic lethal phenotype. Mice that are heterozygous for the Smn1tm1Msd allele and homozygous for the SMN2 transgene are viable, fertile and do not exhibit neuropathy. Mice homozygous for the transgene (and wildtype at the Smn1 locus) do not exhibit an SMA-like phenotype. This mutant mouse strain may be useful in neuromuscular studies involving Spinal Muscular Atrophy (SMA). | ||
| 008630 | B6.Cg-Tg(SMN2)46Tro Smn1tm1Msd/J | Repository- Live |
| Mice that are homozygous for both the Smn1tm1Msd targeted mutation and the SMN2, survival of motor neuron 2, centromeric, human, transgene (founder line 46) are viable, fertile and do not display a SMA-like phenotype. Necrotic lesions are observed on the tail, ears and teeth. Mice that are hemizygous for the transgene and homozygous for the targeted mutation have an embryonic lethal phenotype. Mice that are heterozygous for the Smn1tm1Msd allele and homozygous for the SMN2 transgene are viable, fertile and do not exhibit neuropathy. Mice homozygous for the transgene (and wildtype at the Smn1 locus) do not exhibit an abnormal phenotype. This mutant mouse strain may be useful in neuromuscular studies involving Spinal Muscular Atrophy (SMA). | ||
| 008229 | B6.Cg-Tg(SOD1*G37R)29Dpr/J | Repository- Live |
| Mice hemizygous for this G37R-SOD1 transgene are viable and fertile. The expressed G37R mutant form of human SOD1 is characterized as an enzymatically active, "gain of adverse function" mutation. Hemizygotes develop symptoms and pathology resembling human Amyotrophic Lateral Sclerosis (ALS), with paralyzation in one or more limbs attributable to the loss of motor neurons from the spinal cord. Death occurs around six to eight months of age for transgenic mice on a mixed genetic background. On a congenic C57BL/6 background transgenic mice survive over a year (median lifespan 376 days, Ezzi et al. 2010).
Transgenic mice from this founder line (line 29) express a moderate (7-fold) increase in SOD1 activity in spinal cord, with pathology restricted to motor neurons in the spinal cord and brainstem. Like wild-type SOD1, the G37R mutant SOD1 protein also forms monomer-misfolded oligomers associated with degenerating motor neurons. These G37R-SOD1 transgenic mice may be useful i ..... For more information please see the full phenotype on the strain data sheet | ||
| 008342 | B6.Cg-Tg(SOD1*G37R)42Dpr/J | Repository- Live |
| Mice hemizygous for this G37R-SOD1 transgene are viable and fertile. The expressed G37R mutant form of human SOD1 is characterized as an enzymatically active, "gain of adverse function" mutation. Hemizygotes develop symptoms and pathology resembling human Amyotrophic Lateral Sclerosis (ALS), with paralyzation in one or more limbs attributable to the loss of motor neurons from the spinal cord. Transgenic mice from the highest expressing founder line (G37R(42) or line 42) express a 14-fold increase in SOD1 activity in spinal cord High expression of G37R-SOD1 is associated with ALS pathology in motor neurons of the spinal cord and brainstem, widespread degenerative changes in other neuronal populations, and mild-to-moderate vacuolar changes in kidney. These high-expressing G37R(42) (or G37R-SOD1 line 42) transgenic mice may be useful in studying neuromuscular disorders, including Amyotrophic Lateral Sclerosis (ALS or Lou Gehrig's Disease).
The original publication by Wong et al ..... | ||
| 004435 | B6.Cg-Tg(SOD1*G93A)1Gur/J | Repository- Live |
| Mice hemizygous for this SOD1-G93A (also called G93A-SOD1) transgene are viable and fertile, with transgenic expression of a G93A mutant form of human SOD1. This founder line (often referred to as G1H) is reported to have high transgene copy number. Hemizygotes exhibit a phenotype similar to amyotrophic lateral sclerosis (ALS) in humans; becoming paralyzed in one or more limbs with paralysis due to loss of motor neurons from the spinal cord.
Transgenic mice have an abbreviated life span: 50% survive at 157.1+/-9.3 days (in contrast to the mixed B6SJL background where 50% survival is observed at 128.9+/-9.1 days).
Female hemizygotes are poor breeders, and rarely produce more than one litter before the onset of disease. These SOD1-G93A (also called G93A-SOD1) transgenic mice may be useful in studying neuromuscular disorders, including Amyotrophic Lateral Sclerosis (ALS or Lou Gehrig's Disease).
In an attempt to offer alleles on well-characterized or multiple genetic bac ..... | ||
| 008454 | B6.Cg-Tg(Sox2-cre)1Amc/J | Repository- Live |
| Mice hemizygous for the Sox2Cre transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse SRY-box containing gene 2 promoter. When these transgenic mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in Sox2-expressing tissues in the offspring. Specifically, Cre recombinase activity is detected in the epiblast cells at embryonic day 6.5, with little or no activity in other cells at gastrulation. Some activity is also detected in extra embryonic derivatives of the epiblast, the yolk sac mesoderm and amnion. No Cre recombinase activity is detected in primitive endoderm derived tissues, visceral endoderm. The phenotype of homozygous mice has not been characterized to date (April 2011). These Sox2Cre transgenic may be useful for generating epiblast-derived specific ..... For more information please see the full phenotype on the strain data sheet | ||
| 006361 | B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J | Repository- Live |
| Mice hemizygous for this Osx1-GFP::Cre transgene are viable and fertile.
The transgene carries both tTA under the regulation of the osterix (Sp7) promoter and, just downstream, a tetracycline responsive element (TRE; tetO)-controlled GFP/Cre fusion protein. In the absence of the tetracycline analog doxycycline, EGFP-Cre fusion protein expression is restricted to the osteoblast lineage throughout embryonic and early postnatal development. Fusion protein activity is largely absent from chondrocytes. When these transgenic animals are mated to transgenic strains that carry loxP-flanked (floxed) conditional alleles, Cre-mediated recombination of the floxed allele in the double mutant animals is placed under the regulation of doxycycline (dox) such that dox adminstration prevents fusion protein expression and recombination. The donating investigator suggests that the mice be maintained on dox-treated water to avoid incidental effects of tTA expression (e.g., malocclusion). Th ..... For more information please see the full phenotype on the strain data sheet | ||
| 010905 | B6.Cg-Tg(Sry)2Ei Srydl1Rlb/ArnoJ | Repository- Live |
| The dl1Rlb allele (Y-) is an 11 kb deletion in the sex determining region of the Y chromosome, Sry , XY- mice (with ovaries) with this mutation are phenotypic gonadal females, although they lose germ cells and cease estrous cycling earlier in life. The donating investigator indicates that XY- mice generally infertile on the C57BL/6 background . XX mice carrying the Tg(Sry)2Ei transgene are phenotypic gonadal males (with testes), although they lack sperm and have smaller testes than normal males.
When the two mutations are combined, testis determination is transferred from the Y chromosome to an autosome. Mating the carrier male to a C57BL/6J female produces four "core" genotypes that can be used as a model to investigate relationships between sex chromosome complement (XX or XY) and gonadal type that influences phenotypic characteristics. The four genotypes produced are two types of gonadal females (XX, XY-), and two types ..... For more information please see the full phenotype on the strain data sheet | ||
| 013734 | B6.Cg-Tg(Syn1-ACCN2)1Wsh/J | Repository- Live |
| Human ACCN2 (amiloride-sensitive cation channel 2, neuronal; also called ASIC1a) cDNA is expressed under the control of the rat synapsin I (Syn1) promoter in this transgenic strain. The transgenic protein is highly expressed in neurons throughout the central nervous system. A FLAG tag facilitates immunostaining. In hippocampal neurons from transgenic mice, expression is found in the soma and distributed along dendrites in a punctate pattern. Little or no expression is found in glial cells. Hemizygous mice display enhanced context fear conditioning and have reduced pentylenetetrazol-evoked seizure susceptibility. This strain may be useful in studies of fear and anxiety-related behavior. | ||
| 003966 | B6.Cg-Tg(Syn1-cre)671Jxm/J | Repository- Live |
| These transgenic mice express Cre recombinase under the direction of a synapsin promoter. Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity is detected in neuronal cells by embryonic day 12.5. | ||
| 004128 | B6.Cg-Tg(Tek-cre)12Flv/J | Repository- Live |
| These transgenic mice express Cre recombinase under the direction of the receptor tyrosine kinase Tek promoter/enhancer, which has been shown to provide uniform expression in endothelial cells during embryogenesis and adulthood. Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity results in the deletion of loxP flanked targets in the female germline as well as in endothelial cells and hematopoietic cells. A low frequency of deletion events are also observed by inheritance from the male germline. | ||
| 008863 | B6.Cg-Tg(Tek-cre)1Ywa/J | Repository- Live |
| These transgenic mice express Cre recombinase under the direction of the receptor tyrosine kinase Tek (Tie2) promoter/enhancer, which has been shown to provide uniform expression in endothelial cells during embryogenesis and adulthood. Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity results in the deletion of loxP flanked targets in vascular endothelial cells. | ||
| 008601 | B6.Cg-Tg(Th-cre)1Tmd/J | Repository- Live |
| Mice hemizygous for the TH-Cre transgene are viable and fertile, with the rat tyrosine hydroxylase (TH) promoter directing expression of Cre recombinase to catecholaminergic cells. Using crosses to reporter strains, cre activity is confirmed in catecholaminergic cells and is present in many of the projection areas of these neuronal populations. A mosaic of cre activity is noted in TH-positive neurons. Several other areas that are not typically thought to have active TH expression, including the lateral septal nucleus, accessory olfactory bulb, suparafascicular thalamus, and pretectal area, also exhibit Cre recombinase activity (possibly as a result of TH promoter activity in precursor cell populations or ectopic expression from the exogenous TH promoter). Some TH-negative cells closely clustered around and within TH-positive nuclei demonstrate cre activity. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will re ..... For more information please see the full phenotype on the strain data sheet | ||
| 007901 | B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J | Repository- Live |
| These Thy1-Brainbow 1.0 (line H) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Prior to Cre-mediated recombination, the fluorescent protein immediately adjacent to the promoter, tdimer2(12) (RFP), is expressed in peripheral and central neurons. When bred to Cre recombinase expressing mice, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): tdimer2(12) (RFP) (no recombination), mYFP, or mCerulean (CFP). A palmitoylation sequence tethers the mYFP and mCerulean (CFP) to the membrane, allowing clear labeling of axonal processes, while cytoplasmic tdimer2(12) (RFP) better labeled neuronal cell bodies and dendrites. Integration of tandem transgen ..... For more information please see the full phenotype on the strain data sheet | ||
| 007911 | B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J | Repository- Live |
| These Thy1-Brainbow 1.1 (line M) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Although the fluorescent protein immediately adjacent to the promoter, Kusabira-Orange (kOFP), was designed to be expressed prior to Cre-mediated recombination, basal kOFP expression is not observed in mouse tissues. When bred to Cre recombinase expressing mice, however, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): mCerulean (CFP), mYFP, or mCherry (RFP). The resulting fluorescent protein expression is observed in astrocytes of all areas of the brain and spinal cord, as well as dentate gyrus granule cells. A palmitoylation sequence tethers the mCherry (RFP), ..... For more information please see the full phenotype on the strain data sheet | ||
| 007921 | B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J | Repository- Live |
| These Thy1-Brainbow 2.1 (line R) transgenic mice are viable and fertile. The mice possess two invertible DNA segments (four fluorescent protein sequences in total) uniquely positioned in tandem and flanked with LoxP sites to generate a larger number of recombination outcomes; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Although the fluorescent protein immediately adjacent to the promoter, hrGFPII (with nuclear localization signal), was designed to be expressed prior to Cre-mediated recombination, basal hrGFPII expression may not be observed in mouse tissues. When bred to Cre recombinase expressing mice, however, the resulting offspring can have one of three different inversions for each transgene in each cell of the cre expressing tissue(s). In addition, two excision events may reduce the construct to one of two single invertible DNA segments which can continue to invert as long as cre is present. These different recomb ..... For more information please see the full phenotype on the strain data sheet | ||
| 003710 | B6.Cg-Tg(Thy1-CFP)23Jrs/J | Repository- Live |
| These mice express a spectral variant of GFP (cyan-CFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. This line provides a strong and specific vital marker for axons; fluorescence is also stable to mild aldehyde fixation. Expression is strong from a mid-gestational stage into adulthood. Availability of multiple spectral variants is useful for double-labeling applications, or breeding to other YFP/GFP lines. | ||
| 014131 | B6.Cg-Tg(Thy1-CFP)IJrs/GfngJ | Repository- Live |
| These founder line I transgenic mice express Cyan Fluorescent Protein gene under the control of the mouse Thy1, thymus cell antigen 1, theta, promoter. Fluorescence is detected in all motor axons, all retinal ganglion cells, dorsal root ganglion, neurons in cortical layers 2 through 6, and all of the cerebellar mossy fibers. Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. | ||
| 007940 | B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J | Repository- Live |
| These transgenic mice express Cyan Fluorescent Protein (CFP) under the control of the mouse thymus cell antigen 1, theta, Thy1, promoter. CFP is specifically localized to the mitochondria by a human cytochrome c oxidase, subunit 8A (ubiquitous), targeting signal fused to the N-terminus. Fluorescence is detected in many neuronal populations including retinal cells and in all motor axons. Coronal brain sections reveal a fluorescence pattern showing somatosensory cortex barrel morphology. Neuronal, mitochondrial and neuromuscular junction morphology appears normal in transgenic mice. Axonal mitochondrial density is similar to wildtype. Homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of mitochondrial transport.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different fro ..... | ||
| 007967 | B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J | Repository- Live |
| These transgenic mice express Cyan Fluorescent Protein (CFP) under the control of the mouse thymus cell antigen 1, theta, Thy1, promoter. CFP is specifically localized to the mitochondria by a human cytochrome c oxidase, subunit 8A (ubiquitous), targeting signal fused to the N-terminus. Fluorescence is detected in many neuronal populations including retinal ganglion cells, bipolar cells, amacrine cell and photoreceptors. Neuronal, mitochondrial and neuromuscular junction morphology appears normal in transgenic mice. Axonal mitochondrial density is similar to wildtype. Homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of mitochondrial transport in adult motor neurons.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first chara ..... | ||
| 012597 | B6.Cg-Tg(Thy1-COL25A1)861Yfu/J | Repository- Live |
| Mice hemizygous for the Thy1-COL25A1 allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Collagen XXV alpha 1 (COL25A1), also known as collagen-like Alzheimer's amyloid plaque component precursor, is a type II transmembrane protein expressed in neurons that colocalizes with Amyloid β (Aβ) in senile plaques in the brains of Alzheimer's patients. In the Thy1-COL25A1 mouse, overexpression of human COL25A1 is regulated by a murine Thy1.2 promoter (Thy1), which leads to expression in the cortex and the hippocampus. This overexpression results in accumulation of Aβ and increased levels of p35/p25 and β-site APP-cleaving enzyme 1 (BACE1), which may induce synaptic dysfunction leading to the behavioral changes associated with Alzheimer's Disease. COL25A1 precursor is expressed in cell membranes of cortical neurons, in 2 month old mice. At 6 months, COL25A1 precursor was expressed in t ..... For more information please see the full phenotype on the strain data sheet | ||
| 007612 | B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J | Repository- Live |
| These Thy1-ChR2-YFP founder line 18 transgenic mice express the light-activated ion channel, Channelrhodopsin-2 (from the green alga Chlamydomonas reinhardtii), fused to Yellow Fluorescent Protein (ChR2-YFP) under the control of the mouse thymus cell antigen 1 (Thy1) promoter. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Expression of the transgenic ChR2-YFP fusion protein is detected in layer 5 cortical neurons, CA1 and CA3 pyramidal neurons of the hippocampus, cerebellar mossy fibers, neurons in the thalamus, midbrain and brainstem, and the olfactory bulb mitral cells. Neurons expressing the transgene are morphologically and physiologically comparable to non-mutant neurons. This mutant mouse strain may be useful for ex vivo and in vivo neural circuitry mapping studies using light stimulation. The ChR2-YFP fusion protein is composed of a Chlamydomonas reinhardtii-derived ..... | ||
| 007615 | B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J | Repository- Live |
| These Thy1-ChR2-YFP founder line 9 transgenic mice express the light-activated ion channel, Channelrhodopsin-2 (from the green alga Chlamydomonas reinhardtii), fused to Yellow Fluorescent Protein (ChR2-YFP) under the control of the mouse thymus cell antigen 1 (Thy1) promoter. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Expression of the transgenic ChR2-YFP fusion protein is detected throughout the brain, including in the cortex, hippocampus, thalamus, midbrain, brainstem, cerebellar mossy fibers and retinal ganglion cells. Neurons expressing the transgene are morphologically and physiologically comparable to non-mutant neurons. This mutant mouse strain may be useful for ex vivo and in vivo neural circuitry mapping studies using light stimulation.
The ChR2-YFP fusion protein is composed of a Chlamydomonas reinhardtii-derived channelrhodopsin-2 (ChR2) fused in-frame with an enh ..... | ||
| 013161 | B6.Cg-Tg(Thy1-Clomeleon)1Gjau/J | Repository- Live |
| These transgenic mice express Clomeleon, a fluorescent fusion protein containing CFP and YFP (topaz) that acts as a ratiometric indicator for chloride ions, under the control of the mouse Thy1, thymus cell antigen 1, theta, promoter (Thy1.2). Transgene expression in founder line CLM1 is detected at high levels in the mitral cells of the olfactory bulb, layer 5 of the neocortex, hippocampal dentate gyrus and CA1 region, amygdala, cerebellar granule cells, and retinal bipolar cells. No expression is detected in cerebellar mossy fibers, Purkinje cells or nuclei. In the presence of low chloride ion concentrations, YFP fluoresces as a FRET (Fluorescence Resonance Energy Transfer ) acceptor for CFP. As the chloride ion concentration increases, YFP fluorescence decreases and CFP fluorescence increases. Using an appropriate calibration curve, the ratio of YFP and CFP fluorescence peaks indicate [Cl-]. Mice homozygous for the transgenic insert are viable, fertile, normal in size an ..... For more information please see the full phenotype on the strain data sheet | ||
| 007919 | B6.Cg-Tg(Thy1-EGFP)OJrs/GfngJ | Repository- Live |
| Mice hemizygous for the Thy1-GFP transgene are viable and fertile with enhanced green fluorescent protein (EGFP) expression under the control of a modified Thy1 promoter region (containing the sequences required for neuronal expression but lacking the sequences required for expression in non-neural cells). Thy1-GFP mice derived from founder line O express EGFP in subsets of neurons within specific populations; providing a bright, vital Golgi-like stain. High (>80&) EGFP expression is observed in motor axons and cerebellar mossy fibers. Low (<10%) EGFP expression is observed in retinal ganglion cells and lumbar dorsal root ganglion. Sparse EGFP-labeling is also observed in neurons from multiple cortical layers. These Thy1-GFP line O transgenic mice may be useful for fluorescent labeling of neural tissues; especially motor axons and cerebellar mossy fibers, as well as intense, yet sparse, labeling of a variety of cortical neurons.
This strain is one of many from the same transgene ..... | ||
| 003709 | B6.Cg-Tg(Thy1-YFP)16Jrs/J | Repository- Live |
| These mice express spectral variants of GFP (yellow-YFP) at high levels in motor and sensory neurons, as well as in subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. The transgenic insert used to make this strain is identical to that used in the construction of Stock No. 003782. This line provides a strong and specific vital marker for axons; fluorescence is also stable to mild aldehyde fixation. Expression is strong from a mid-gestational stage into adulthood. Availability of multiple spectral variants is useful for double-labeling applications, or breeding to other CFP/GFP lines. | ||
| 014130 | B6.Cg-Tg(Thy1-YFP)GJrs/GfngJ | Repository- Live |
| These founder line G (or 17) transgenic mice express Yellow Fluorescent Protein gene under the control of the mouse Thy1, thymus cell antigen 1, theta, promoter. Fluorescence is detected in all motor axons, many retinal ganglion cells, more than 80% of pre superior cervical ganglion neurons, less than 10% of post superior cervical ganglion neurons, neurons in cortical layers 2 through 6, and the cerebellar mossy fibers in the internal granule layer. Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. | ||
| 003782 | B6.Cg-Tg(Thy1-YFP)HJrs/J | Repository- Live |
| These mice express spectral variants of GFP (yellow-YFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. The transgenic insert used to make this strain is identical to that used in the construction of strain 003709. The primary difference between these two strains is the specific neuron subsets which express YFP. In this strain, a few motor axons are labeled in muscle tissue, allowing determination of branching pattern and definition of which muscle fibers are innervated by a single motor axon. Approximately 10-30% of sensory neurons are labeled in dorsal root ganglia. Layer 5 pyramidal cells are selectively labeled in cerebral cortex. Pyramidal neurons are selectively labeled in the hippocampus. Approximately 10-30% of retinal ganglion cells are exclusively labeled in the retina. Many (but not all) mossy fibers are strongly labeled in cerebel ..... For more information please see the full phenotype on the strain data sheet | ||
| 007606 | B6.Cg-Tg(Thy1-cre/ERT2,-EYFP)AGfng/J | Repository- Live |
| These transgenic mice ("SLICK" for single-neuron labeling with inducible Cre-mediated knock-out) have a tamoxifen inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted neuron-specific deletions only in neurons where the Thy1 promoter is active. These cells will be evident by the expression of Yellow Fluorescent Protein. Transgenic mice express YFP constitutively in neurons. The Donating Investigator reports that founder line A mice express the transgene in small subsets of motor neurons and dorsal root ganglion neurons.
..... For more information please see the full phenotype on the strain data sheet | ||
| 013196 | B6.Cg-Tg(Tie2-B4galnt2)1108Dgi/J | Repository- Live |
| Tie2-B4galnt2 transgenic mice have beta-1,4-N-acetyl-galactosaminyl transferase 2 (B4galnt2) expression being driven by the vascular endothelial-specific receptor tyrosine kinase (Tie2) promoter in vascular endothelial cells. Hemizygous mice are viable, fertile, and normal in size. These mice exhibit lower plasma von Willebrand factor (VWF) levels and prolonged bleeding time characteristic of RIIIS/J mice (Stock No. 000683), which contain a modified VWF (Mvwf) gene. These mice may be useful for studying plasma VWF levels, cohesion and aggregation of platelets at the site of injury, bleeding, and clotting associated with human von Willebrand disease. | ||
| 012328 | B6.Cg-Tg(Tyr-cre/ERT2)13Bos/J | Repository- Live |
| Homozygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cre-ERT2 fusion gene activity can be induced following tamoxifen or 4-hydroxytamoxifen administration. When Tyr::CreERT2 mice are bred with mice containing loxP-flanked sequences, inducible Cre-mediated recombination will result in deletion of the floxed sequences in the Tyr-expressing cells of the offspring. The donating investigator reports that Cre recombinase activity is observed in embryonic melanoblasts, follicular bulb melanocytes, dermal dendritic melanocytes, epidermal melanocytes of tail skin and ocular chorid cells. Recombinase expression was not observed in the other major tissues/organs tested. This mutant mouse strain may be useful in studies of melanocyte development, melanocyte stem cell function and melanomas. | ||
| 015805 | B6.Cg-Tg(UBC-GFP,-TVA)1Clc/J | Repository- Live |
| The cTVA transgene contains the human ubiquitin C (UBC) promoter/enhancer elements drive expression of a loxP-flanked green fluorescent protein (GFP) and polyadenylation sequence, followed by an avian specific retroviral receptor (TVA) gene derived from quail. Hemizygous mice are viable and fertile. UBC is a polyubiquitin precursor responsible for the regulation of cell signaling pathways upon conjugation with various proteins. In this mutant mouse, GFP is expressed in UBC-expressing cells. When bred to mice that express Cre recombinase, offspring will have the floxed-GFP-STOP cassette deleted in the cre-expressing tissue(s), resulting in TVA overexpression in UBC-expressing cells. Upon expression of TVA, these cells are capable of binding viruses with the envelope protein of avian sarcoma/leukosis virus subtype A (ASLV-A). These cTVA mice may be useful for infection by retroviruses, rabies virus, and other viruses with ASLV-A ..... For more information please see the full phenotype on the strain data sheet | ||
| 015806 | B6.Cg-Tg(UBC-GFP,-TVA)2Clc/J | Repository- Live |
| The cTVA transgene contains the human ubiquitin C (UBC) promoter/enhancer elements drive expression of a loxP-flanked green fluorescent protein (GFP) and polyadenylation sequence, followed by an avian specific retroviral receptor (TVA) gene derived from quail. Hemizygous mice are viable and fertile. UBC is a polyubiquitin precursor responsible for the regulation of cell signaling pathways upon conjugation with various proteins. In this mutant mouse, GFP is expressed in UBC-expressing cells. When bred to mice that express Cre recombinase, offspring will have the floxed-GFP-STOP cassette deleted in the cre-expressing tissue(s), resulting in TVA overexpression in UBC-expressing cells. Upon expression of TVA, these cells are capable of binding viruses with the envelope protein of avian sarcoma/leukosis virus subtype A (ASLV-A). These cTVA mice may be useful for infection by retroviruses, rabies virus, and other viruses with ASLV-A ..... For more information please see the full phenotype on the strain data sheet | ||
| 015807 | B6.Cg-Tg(UBC-GFP,-TVA)3Clc/J | Repository- Live |
| The cTVA transgene contains the human ubiquitin C (UBC) promoter/enhancer elements drive expression of a loxP-flanked green fluorescent protein (GFP) and polyadenylation sequence, followed by an avian specific retroviral receptor (TVA) gene derived from quail. Hemizygous mice are viable and fertile. UBC is a polyubiquitin precursor responsible for the regulation of cell signaling pathways upon conjugation with various proteins. In this mutant mouse, GFP is expressed in UBC-expressing cells. When bred to mice that express Cre recombinase, offspring will have the floxed-GFP-STOP cassette deleted in the cre-expressing tissue(s), resulting in TVA overexpression in UBC-expressing cells. Upon expression of TVA, these cells are capable of binding viruses with the envelope protein of avian sarcoma/leukosis virus subtype A (ASLV-A). These cTVA mice may be useful for infection by retroviruses, rabies virus, and other viruses with ASLV-A ..... For more information please see the full phenotype on the strain data sheet | ||
| 015808 | B6.Cg-Tg(UBC-TVA)1Clc/J | Repository- Live |
| The TVA transgene contains the human ubiquitin C (UBC) promoter driving expression of an avian specific retroviral receptor (TVA) gene derived from quail. Hemizygous mice are viable and fertile. UBC is a polyubiquitin precursor responsible for the regulation of cell signaling pathways upon conjugation with various proteins. Expression of TVA in these cells allows the binding of viruses with the envelope protein of avian sarcoma/leukosis virus subtype A (ASLV-A). These TVA mice may be useful for infection by retroviruses, rabies virus, and other viruses with ASLV glycoproteins. | ||
| 008085 | B6.Cg-Tg(UBC-cre/ERT2)1Ejb/J | Repository- Live |
| Mice hemizygous for this Cre-ERT2 transgene are viable and fertile. Mice from this founder line have strong tamoxifen-inducible cre activity in all reported tissue types. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to OHT. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in widespread cel ..... For more information please see the full phenotype on the strain data sheet | ||
| 008610 | B6.Cg-Tg(Vav1-cre)A2Kio/J | Repository- Live |
| Mice hemizygous for this Vav-iCre transgene are viable and fertile, with the mouse HS21/45-vav control regions directing expression of an optimized variant of Cre recombinase (iCre) to hematopoietic cells (and their progenitors). Using crosses to a reporter strain, variegated germ line (testis and ovaries), and heart and gut expression is also reported. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Vav-iCre transgenic mice may be useful for generating conditional mutations in hematopoietic cells.
Of note, this Vav-iCre strain (Stock No. 008610) allows reliable deletion of specific genes throughout the entire hematopoietic compartment, whereas the hCD2-iCre strain (Stock No. 008520) allows targeting to be focused to T cells and B cells. | ||
| 009614 | B6.Cg-Tg(Wfs1-cre/ERT2)2Aibs/J | Repository- Live |
| Hemizygous Wfs1-Tg2-CreERT2 mice are viable and fertile. As the Cre-ERT2 fusion gene is under control of the Wfs1 promoter/enhancer regions within the BAC transgene, cre activity is directed to cortex, hippocampus, and cerebellum only following tamoxifen administration. The donating investigators report that Cre recombinase expression for this Wfs1-Tg2-CreERT2 line is more restricted than the Wfs1-Tg3-CreERT2 line (Stock No. Stock No. 009103). The donating investigators may not have assessed expression in tissues other than brain. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only ga ..... For more information please see the full phenotype on the strain data sheet | ||
| 009107 | B6.Cg-Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J | Repository- Live |
| In 2011, Stock No. 009107 at The Jackson Laboratory Repository was confirmed to harbor the expected Wnt1-Cre transgene, as well as the originally co-injected Wnt1-GAL4 transgene. The strain phenotype description below has been updated accordingly.
When homozygous for both co-injected transgenes, Wnt-1/GAL4/cre-11 transgenic mice are viable, fertile, normal in size and do not display any gross physical abnormalities. Of note for Wnt-1/GAL4/cre-11 transgenic mice on the original mixed genetic background, the donating investigator (Dr. Epstein) reported that homozygous mice may be lethal as some offspring from transgenic parents die around two months of age. Both Cre recombinase and the GAL4 transcriptional activator are expressed under the direction of wingless-related MMTV integration site 1 (Wnt1) promoter/regulatory sequences. Cre recombinase activity is detected in the Wnt1 pattern of expression: in the midbrain by 8.5 dpc and, after neural ..... | ||
| 008468 | B6.Cg-Tg(tetO-DTA)1Gfi/J | Repository- Live |
| These tet-DTA transgenic mice express diphtheria toxin A (DTA) under the control of a tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) and a cytomegalovirus minimal promoter. When bred with another mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue diphtheria toxin A expression can be regulated with the tetracycline analog doxycycline (dox) in the resulting double mutant offspring. These tet-DTA mice may be useful in generating bi-transgenic mutant mice for the reversible, inducible deletion of specific groups of cells.
For example, when bred to a strain expressing tTA in cardiac myocytes (see Stock No. 003170 for example), this mutant mouse strain may be useful in studies of human cardiomyopathies. When bred to a strain expressing tTA in pancreatic beta cells (see Stock N ..... | ||
| 006234 | B6.Cg-Tg(tetO-cre)1Jaw/J | Repository- Live |
| Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of a tetracycline-responsive promoter element (TRE; tetO). When hemizygotes are bred with another transgenic mouse expressing either reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under the control of tissue-specific promoters, Cre recombinase expression and Cre-mediated recombination in the appropriate tissues of the bitransgenic offspring can be regulated with the tetracycline analog, doxycycline. This strain represents an effective tool for generating inducible tissue specific-targeted mutants to study cell lineage during development.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was fi ..... | ||
| 005657 | B6.FVB(129)-Tg(Myh6-cre/Esr1*)1Jmk/J | Repository- Live |
| The alpha-MHC-MerCreMer transgene has the mouse Myh6 promoter (myosin, heavy polypeptide 6, cardiac muscle, alpha; alpha-MHC) directing expression of a tamoxifen-inducible Cre recombinase (MerCreMer) to juvenile and adult cardiac myocytes. Mice homozygous for the alpha-MHC-MerCreMer transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre recombinase expression in heart tissue is confirmed by western blot. Southern blot confirmed heart cell specificity compared to brain, kidney, lung, liver, and skeletal muscle. Insertion of this transgene and its protein show no changes in echocardiography, heart mass or pathology, or hypertrophy marker genes compared to nontransgenic littermates. Of note, the MerCreMer double fusion protein has substantially greater Cre recombinase activity with less promiscuity compared with the CreMer single fusion protein. When alpha-MHC-MerCreMer transgenic mice are bred with mice containing For more information please see the full phenotype on the strain data sheet | ||
| 006475 | B6.FVB(129S4)-Tg(Ckmm-cre)5Khn/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice have the Cre recombinase gene driven by the muscle creatine kinase (MCK or Ckm) promoter. Cre activity is observed in skeletal and cardiac muscle. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in skeletal and cardiac muscle deletion of the flanked genome. | ||
| 007084 | B6.FVB(Cg)-Mmp9tm1Tvu/J | Repository- Live |
| Mice that are homozygous null for the Mmp9 gene are viable and fertile. No Mmp9 activity is detected in spleen cell lysates. Long bones (tibia, femur) are 10% shorter in homozygous null mice. Histological examination of three-week-old mice reveals a dramatically lengthened zone of hypertrophic cartilage (six to eight times larger vs. wild-type) due to delayed apoptosis, vascularization, and ossification. Subsequent remodeling resolves the condition, resulting in normal appearing bones by eight weeks of age. Null mice show altered responses to repair of injury in skin, cornea, central nervous system and bone marrow reconstitution, and altered inflammatory responses.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain de ..... | ||
| 006333 | B6.FVB(Cg)-Tg(Neurog3-cre)C1Able/J | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Expression of the transgene is directed by a neurogenin 3 promoter. Tissues where Cre recombinase expression is detected include the small intestine (base of intestinal crypts) and fetal pancreatic epithelial cells. Cre activity has been shown in islets of the adult pancreas, small intestine enteroendocrine cells, endocrine portions of the stomach, all pancreatic endocrine cells, and in some non-endocrine intestinal cells. When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked gene in the tissues that normally express neurogenin 3.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be not ..... | ||
| 014643 | B6.FVB-Tg(CMA1-cre)6Thhe/J | Repository- Live |
| These transgenic mice express Cre recombinase under the direction of the baboon CMA1, chymase 1, mast cell, promoter. Cre recombinase transcript (mRNA) is detected in lung and colon analyzed by RT-PCR. Transgene expression (mRNA by RT-PCR) and Cre recombinase activity is not detected in skin or heart tissues. Recombinase activity is detected in lung and colon tissue, specifically in resident mast cells. Transgene expression and Cre recombinase activity is not detected in cultured bone marrow derived mast cells by RT-PCR analysis or FACS analysis. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator reports that homozygotes are not viable. | ||
| 003724 | B6.FVB-Tg(EIIa-cre)C5379Lmgd/J | Repository- Live |
| This line carries a cre transgene under the control of the adenovirus EIIa promoter that targets expression of Cre recombinase to the early mouse embryo. Cre expression is thought to occur prior to implantation in the uterine wall. A mosaic pattern of expression is commonly observed. Cre-mediated recombination occurs in a wide range of tissues, including the germ cells that transmit the genetic alteration to progeny. These mice may be useful for breeding to other mice carrying loxP-flanked DNA sequences of interest. This would readily generate progeny in which Cre-mediated excision of the targeted sequences has occurred.
View cre expression characterization. | ||
| 011069 | B6.FVB-Tg(Gh1-cre)bKnmn/J | Repository- Live |
| These transgenic mice express Cre recombinase under the control of the rat growth hormone gene, Gh1. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cre recombinase gene product (mRNA) is detected in the pituitary and, at lower levels, in the testis by RT-PCR of tissues from 8-10 week old mice. Cre recombinase expression is also detected by RT-PCR analysis of cephalic extracts from hemizygous embryos aged embryonic day 17. Recombination is detected in the anterior pituitary gland, in the somatotrope cells and a subset of the lactotrope cells. Cre recombinase expression is not detected in the ovary, hypothalamus, cortex, cerebelum, heart, lung, liver, spleen, kidney, adrenal, pancreas, stomach, adipose or uterus. | ||
| 006000 | B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J | Repository- Live |
| Mice that are homozygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice have a diphtheria toxin (DT) inducible system that transiently depletes macrophages in various tissues. The transgene insert contains a fusion product involving simian diphtheria toxin receptor and green fluorescent protein under the control of the human ITGAM (integrin alpha M) promoter (CD11b). RT-PCR analysis of bone marrow macrophages detects specific transgene expression. Cytological analysis of thioglycollate treated peritoneal cells shows the absence of macrophages. Intraperitoneal injection of DT ablates monocyte/macrophage cells in the peritoneal cavity. Macrophage populations within various tissues demonstrate differential susceptibility DT induced deletion. Following DT administration macrophages are ablated in the peritoneum, kidney and ovary. Macrophage population is restored by day 4 following a single intr ..... For more information please see the full phenotype on the strain data sheet | ||
| 004509 | B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J | Repository- Live |
| Mice that are homozygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. Upon diphtheria toxin (DT) administration, mice harboring this transgene are transiently depleted of dendritic cell (DC) populations. All CD8+ and CD8- DC in the spleen express EGFP and are DT sensitive. Immunohistochemical and flow cytometric analysis reveals EGFP expression and DT-inducible depletion of CD11chigh DC in spleen, lymph node, lung, liver and lamina propria tissues, as well as the defined macrophage subpopulations of the alveolar, lamina propria, metallophillic and marginal zone. Rapid reduction of CD11c+ DC populations induced by intraperitoneal injection of DT persists for approximately 2 days, after which the cell population gradually recovers. While transient DC depletion was not associated with sign of illness or long-term defects, repeated DT induction is lethal to the mouse. Long term de ..... For more information please see the full phenotype on the strain data sheet | ||
| 012446 | B6.FVB-Tg(LRRK2*G2019S)1Mjfa/J | Repository- Live |
| These transgenic mice express the mutant human LRRK2 sequence, 6055G->A transition (G2019S), with high levels of protein detected in an expression pattern similar to that of the endogenous mouse Lrrk2. The transgene inserted into Chromosome 13. Transgenic mice express mutant human LRRK2 at approximately 8-10 fold over the endogenous mouse Lrrk2 level. Mice that are homozygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of Parkinson's disease. | ||
| 011038 | B6.FVB-Tg(Myh6-cre)2182Mds/J | Repository- Live |
| The cardiac-specific murine alpha myosin-heavy chain (Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter drives expression of cre in this transgenic strain. Breeding this mouse with another carrying loxP-flanked sequences results in the deletion of the flanked sequences in the offspring. The promoter induces greater than 90% recombination in cardiac muscle cells. No recombination is observed in liver, lung, skeletal muscle (quadriceps), and spleen, or in extraneous cell types in the heart. | ||
| 013781 | B6.FVB-Tg(Myh6/NFAT-luc)1Jmol/J | Repository- Live |
| These nuclear factor of activated T cell (NFAT)-luciferase transgenic mice display detectable luciferase activity in most tissues surveyed at 3 weeks of age, with highest expression occurring in brain, kidney, and heart - each of which are sites of considerable calcineurin (Ppp3ca, protein phosphatase 3, catalytic subunit, alpha isoform) protein expression. NFAT-luciferase activity peaks during developmental maturation of the heart, whereas the adult heart shows relatively lower activity. In the adult heart, NFAT-luciferase activity is upregulated in a delayed, but sustained manner throughout 8 weeks of pathological cardiac hypertrophy induced by pressure-overload, or more dramatically following myocardial infarction-induced heart failure. This strain may be useful in studies of cardiac hypertrophy and calcineurin/NFAT coupling. | ||
| 006417 | B6.FVB-Tg(Npy-hrGFP)1Lowl/J | Repository- Live |
| Hemizygous mice are viable and fertile. These mice express humanized Renilla Green Fluorescent Protein (hrGFP, Stratagene) under control of the mouse neuropeptide Y (Npy) promoter. As such, UV light-exposed transgenic brain tissues show GFP fluorescence patterns consistent with the (Npy) gene. The donating investigator reports that the hrGFP expressed from this transgene is more stable and resistant to signal fading compared to other GFP’s. These transgenic mice may be useful for studies of neurobiology, energy metabolism, obesity, seizures, and epilepsy. | ||
| 010714 | B6.FVB-Tg(Pomc-cre)1Stl/J | Repository- Live |
| In this strain, the mouse pro-opiomelanocortin-alpha (Pomc) promoter drives expression of cre in the central nervous system, primarily the hippocampus. Cre expression is strongest and most restricted to the granule cells of the dentate gyrus subregion. Weaker, scattered expression can also be detected in other regions including the arcuate nucleus of the hypothalamus and the habenular nucleus. When crossed with a strain carrying a loxP-flanked genomic segment of interest, tissue-specific excision of that segment may be achieved. | ||
| 008126 | B6.NOD-Tg(Cd4-EGFP)1Lt/J | Repository- Live |
| The donating investigator reports that mice homozygous for this CD4-GFP transgene are viable, fertile, normal in size and do not exhibit any gross physical or behavioral abnormalities. FACS analysis of splenic lymphocytes shows transgenic expression uniformly in 81% of inactivated or resting CD8+, 81% on inactivated or resting CD4+, and minimal expression in B cells (2.3%) and macrophages (1.5%). Antigen activated CD4+ T cells continue to express GFP, while activated CD8+ T cells lost GFP expression. These CD4-GFP transgenic mice may be useful for fluorescent monitoring of T cells both in vivo and in vitro.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described on a NOD/ ..... | ||
| 004586 | B6.SJL-Tg(Vil-cre)997Gum/J | Repository- Live |
| Mice hemizygous for this transgene express Cre recombinase under the direction of the mouse villin 1 promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in villi and crypt cells of the small and large intestines, closely patterning the endogenous gene expression. The Donating Investigator indicates that expression is generally continuous, but that a small amount of mosaicism is noted in the colon. Onset of transgene expression is at 12.5 dpc, which is delayed from the endogenous mouse Vil1 gene expression onset of 9.0 dpc. Hemizygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of intestinal organogenesis. | ||
| 008529 | B6;129P-Tg(Neurog1-cre/ERT2)1Good/J | Repository- Live |
| These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse neurogenin 1, Neurog1, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/ESR1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in the cortex, hippocampus, thalamus, hypothalamus and cochlear-vestibular ganglion. Hemizygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnorma ..... For more information please see the full phenotype on the strain data sheet | ||
| 014605 | B6;129S-Tg(CMV-BBS1)6Vcs/J | Repository- Live |
| The CMV-BBS1 transgene contains a simian cytomegalovirus (sCMV) IE94 promoter driving expression of the open reading frame of the human Bardet-Biedl syndrome 1 (BBS1) gene, tagged with a 5' 3xFLAG tag and 4xMyc tag. Homozygous mice are viable and fertile. BBS is a pleiotropic disorder characterized by retinal and photoreceptor degeneration, obesity, polydactyly, renal abnormalities, hypogenitalism and cognitive impairment. BBS is associated with an increased incidence of hypertension, diabetes mellitus and heart defects. These mice are phenotypically normal and express the transgene in the eye and testes. | ||
| 007001 | B6;129S-Tg(UBC-cre/ERT2)1Ejb/J | Repository- Live |
| Mice hemizygous for this Cre-ERT2 transgene are viable and fertile. Mice from this founder line have strong tamoxifen-inducible cre activity in all reported tissue types. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to OHT. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in widespread cel ..... For more information please see the full phenotype on the strain data sheet | ||
| 012362 | B6;129S6-Tg(Camk2a-cre/ERT2)1Aibs/J | Repository- Live |
| Mice hemizygous for the Camk2a-CreERT2 transgene are viable and fertile, with expression of CreERT2 fusion protein (CreERT2 fusion protein) directed to neural populations by the mouse calcium/calmodulin-dependent protein kinase II alpha promoter region. Cre-ERT2 fusion gene activity is inducible; observed at high levels following tamoxifen administration. When Camk2a-CreERT2 transgenic mice are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in the Camk2a-expressing cells of the double mutant offspring. Specifically, the donating investigator reports that the Camk2a-CreERT2 transgene directs reporter gene expression in sparse populations of neurons in the cortex, hippocampus, striatum, and other structures in the absence of tamoxifen. Following tamoxifen administration, reporter gene expression is turned on in widespread populations of neurons in the same regions ..... For more information please see the full phenotype on the strain data sheet | ||
| 012433 | B6;C3-Tg(ACTA1-rtTA,tetO-cre)102Monk/J | Repository- Live |
| These transgenic mice have a tetracycline (doxycycline) inducible Cre-mediated recombination system that is specific for skeletal muscle myocytes. Two transgenic constructs were coinjected to generate this strain. The first transgene contains cre recombinase under the control of the tetO, tetracycline-responsive regulatory element and a second transgenic construct contains the reverse tetracycline-controlled transactivator, rtTA (Tet-On), under the control of the human ACTA1, actin, alpha 1, skeletal muscle promoter. Mice hemizygous for the transgenic inserts are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cre mRNA expression is detected in mice treated with doxycycline (dox) specifically in limb muscle. In the absence of dox, very weak Cre mRNA expression is detected in skeletal muscle. When crossed with a reporter strain, inducible Cre recombinase activity is restricted to skeletal muscle tissues. Slight Cre ..... For more information please see the full phenotype on the strain data sheet | ||
| 008605 | B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J | Repository- Live |
| Hemizygous IRG transgenic mice are viable and fertile, with widespread expression of a loxP-flanked optimized red fluorescent protein variant (DsRed-Express) directed to embryonic and adult tissues by the CAG promoter prior to exposure to Cre recombinase. When bred to cre-expressing mice, the resulting offspring have the DsRed-Express cassette deleted in the cre-expressing tissue(s), allowing expression of the enhanced green fluorescent protein (EGFP) cassette located just downstream. These IRG transgenic mice are a double-fluorescent, Cre-reporter strain; with widespread expression of red fluorescence prior to Cre recombinase exposure, and green fluorescence following cre-mediated recombination in a pattern determined by cre expression, and should provide a versatile tool for analyzing complex cellular relationships in a wide variety of tissues. For example, when using IRG transgenic mice along with Nestin-Cre mice (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 010827 | B6;C3-Tg(FOXJ1-EGFP)85Leo/J | Repository- Live |
| These transgenic mice express Green Fluorescent Protein in ciliated cells of tracheal, bronchial, nasal epithelium, oviduct, testis and in ciliated ependymal cells lining brain ventricles. Transgene expression is restricted to ciliated cells as detected by immunohistochemical analysis of formaldehyde-fixed, paraffin-embedded sections of tissue containing ciliated epithelium and confocal microscopy of thick sections of formaldehyde-fixed, agarose embedded tissue. In mature cultures of tracheal epithelial cells, all ciliated cells are GFP fluorescent and all GFP fluorescent cells are ciliated. In vitro, transgene expression coincides with onset of centriole formation. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of development, physiology and pathology of the lung and other tissues with ciliated epithelium. | ||
| 016575 | B6;C3-Tg(PDGFB-LRRK2*G2019S)340Djmo/J | Repository- Live |
| G2019S-LRRK2 transgenic mice have a minimal cytomegalovirus (CMV) enhancer and human platelet derived growth factor, B polypeptide (PDGFB) promoter/enhancer elements driving expression of a mutated full length human leucine-rich repeat kinase 2 (LRRK2*G2019S) cDNA. Hemizygotes are viable, fertile, and normal in size. The LRRK2 cDNA was modified to harbor the LRRK2*G2019S mutation associated with autosomal dominant, late-onset Parkinson's Disease (PD) originally identified in multiple Spanish families and patients with PD. LRRK2 protein, also known as Dardarin, contains multiple functional domains and may play a role in regulating alpha-synuclein-mediated neuropathology through modulating the intracellular trafficking and accumulation of SNCA protein. G2019S-LRRK2 is expressed throughout the olfactory bulb, cerebral cortex, hippocampus, striatum, cerebellum, and neurons of the substantia nigra pars compacta. G2019S-LRRK2 is also overexpressed (2.7-f ..... For more information please see the full phenotype on the strain data sheet | ||
| 016576 | B6;C3-Tg(PDGFB-LRRK2*R1441C)574Djmo/J | Repository- Live |
| R1441C-LRRK2 transgenic mice have a minimal cytomegalovirus (CMV) enhancer and human platelet derived growth factor, B polypeptide (PDGFB) promoter/enhancer elements driving expression of a mutated full length human leucine-rich repeat kinase 2 (LRRK2*R1441C) cDNA. Hemizygotes are viable, fertile, and normal in size. The LRRK2 cDNA was modified to harbor the LRRK2*R1441C mutation associated with autosomal dominant, late-onset Parkinson's disease originally identified in family D from Western Nebraska. LRRK2 protein, also known as Dardarin, contains multiple functional domains and may play a role in regulating alpha-synuclein-mediated neuropathology through modulating the intracellular trafficking and accumulation of SNCA protein. R1441C-LRRK2 is selectively overexpressed in the cerebral cortex and cerebellum. These mice display reduced levels of cortical catecholamines, a progressive impairment of locomotor activity and the accumulation of autopha ..... For more information please see the full phenotype on the strain data sheet | ||
| 008169 | B6;C3-Tg(Prnp-MAPT*P301S)PS19Vle/J | Repository- Live |
| These transgenic mice express the P301S mutant human microtubule-associated protein tau, MAPT, under the direction of the mouse prion protein, Prnp, promoter. The expression of the mutant human MAPT is five-fold higher than the expression of the endogenous mouse MAPT protein. Hyperphosphorylated, insoluble mutant human MAPT protein in the brain accumulates with age causing decreased microtubule binding. At three months of age, transgenic mice exhibit clasping and limb retraction when lifted by the tail, which progresses to limb weakness. By 10 months of age the mice exhibit a hunched back and paralysis, followed by inability to feed. Transgenic mice have a median lifespan of approximately nine months with approximately 80% dying by 12 months. Histological analysis reveals neuron degeneration in hippocampus and ventricular dilatation (brain atrophy) by eight months of age, although significant neuron degeneration in the hippocampus occurs at approximately nine months of a ..... For more information please see the full phenotype on the strain data sheet | ||
| 004479 | B6;C3-Tg(Prnp-SNCA*A53T)83Vle/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable and normal in size. These transgenic mice express human A53T variant alpha-synuclein (full-length, 140 amino acid isoform) under the direction of the mouse prion protein promoter. At eight months of age, some homozygous mice develop a progressively severe motor phenotype. Presentation of the phenotype may manifest at 14-15 months of age (on average). Lax grooming, weight loss and diminished mobility precede movement impairment, partial limb paralysis, trembling and inability to stand. Immunohistochemistry analysis of mutants between eight to 12 months of age reveals widely distributed alpha-synuclein inclusions, with dense accumulation in the spinal cord, brainstem, cerebellum and thalamus. The appearance of alpha-synuclein aggregate inclusions parallels the onset of the motor impairment phenotype. Axons and myelin sheaths exhibit progressive ultrastructural degeneration. Immunoelectron microscopy and biochemical analysis show the in ..... For more information please see the full phenotype on the strain data sheet | ||
| 009613 | B6;C3-Tg(Scnn1a-cre)3Aibs/J | Repository- Live |
| Hemizygous Scnn1a-Tg3-Cre mice are viable and fertile, with cre expression directed to cortex, thalamus, midbrain, and cerebellum by the Scnn1a promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Scnn1a-Tg3-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex, thalamus, midbrain, and cerebellum).
For characterization information, see images at the Allen Institute for Brain Science website (Scnn1a-Tg3-Cre images). | ||
| 009103 | B6;C3-Tg(Wfs1-cre/ERT2)3Aibs/J | Repository- Live |
| Hemizygous Wfs1-Tg3-CreERT2 mice are viable and fertile. As the Cre-ERT2 fusion gene is under control of the Wfs1 promoter/enhancer regions within the BAC transgene, cre activity is directed to cortex, hippocampus, striatum, thalamus and cerebellum only following tamoxifen administration. The donating investigators may not have assessed expression in tissues other than brain. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone ma ..... For more information please see the full phenotype on the strain data sheet | ||
| 014650 | B6;C3-Tg(tetO-TARDBP*)4Vle/J | Repository- Live |
| These transgenic mice express the mutant human TARDBP, TAR DNA binding protein, hTDP-43-deltaNLS, with a defective nuclear localization signal, under the direction of the tetO, tetracycline operator promoter. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. It is not known if homozygotes are viable. When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of the hTDP-43-deltaNLS protein may be regulated with tetracycline or its analog doxycycline (DOX) in the double mutant offspring. When mated to Camk2a-tTA mice which express tTA in forebrain neurons (See for example: Stock No. 003010; Stock No. 007004; Stock No. 010712), the resulting bitransgenic mice ex ..... For more information please see the full phenotype on the strain data sheet | ||
| 012705 | B6;CBA-Tg(ATXN3*)84.2Cce/IbezJ | Repository- Live |
| MJD84.2 transgenic mice (also called SCA3-YAC-84Q transgenic mice) are viable and fertile. These mice harbor a YAC transgene that expresses a human ataxin 3 (ATXN3; also called Machado-Joseph disease (MJD), MJD1, or spinocerebellar ataxia 3 (SCA3)) gene modified with an expanded 84 CAG repeat motif that is associated with MJD/SCA3 in humans. Hemizygous mice (MJD84.2) harbor two copies of the transgene at a single genomic integration site, with transgene expression levels and patterns almost identical to endogenous MJD. Transgene expression is widespread (detected in the cerebellum, cerebral cortex, heart, lung, spleen, liver, and skeletal muscle). Stable transmission of the MJD1/CAG84 transgene has been demonstrated for multiple generations with a predicted frequency of about 50%. Hemizygous mice exhibit attenuated weight gain and a progressive neurological phenotype. The neurological phenotype is characterized by prominent gait abnormalities (~ 4 weeks), mild tremor, moderate ..... For more information please see the full phenotype on the strain data sheet | ||
| 003010 | B6;CBA-Tg(Camk2a-tTA)1Mmay/J | Repository- Live |
| Transgenic mice expressing the tetracycline-controlled transactivator protein (tTA) under regulatory control of the forebrain-specific calcium-calmodulin-dependent kinase II (Camk2a) promoter are viable, fertile, and display no overt phenotypic defects. Transgene expression can be blocked by the administration of the tetracycline analog doxycycline (dox) to the mice. Mating these transgenic mice to a second transgenic strain carrying a gene of interest coupled to a tetracycline-responsive promoter element (TRE; tetO) allows dox-inducible expression of the target gene specifically in forebrain neurons, and may be useful in studying brain disorders such as Alzheimer's disease (when used in conjunction with Stock No. 005706, Stock No. 007049, Stock No. 007051, Stock No. 00 ..... For more information please see the full phenotype on the strain data sheet | ||
| 014647 | B6;CBA-Tg(Pdx1-cre)6Cvw/J | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse Pdx1 (pancreatic and duodenal homeobox 1) promoter. Mosaic Cre recombinase activity is detected in the pancreatic epithelium, antral stomach and duodenum in neonates and in pancreatic beta islet cells in adults. No Cre recombinase activity is detected ectopically to the Pdx1 expression domain. When crossed with a strain containing loxP site-flanked sequences, Cre-mediated recombination results in deletion of the floxed sequences in the cre-expressing tissues of the offspring. It is not known if homozygotes are viable. | ||
| 004654 | B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein under the control of the POU domain, class 5, transcription factor 1, promoter and distal enhancer. Primordial germ cell specific markers, alkaline phosphatase II and stage-specific embryonic antigen, are co-expressed in EGFP positive cells. 9.5 and 10.5 dpc (days post-coitum) migratory primordial germ cells from hemizygotes and homozygotes can be sorted and isolated by flow cytometry. This strain represents an effective tool for studying genetic imprinting and early embryonic development. | ||
| 007910 | B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J | Repository- Live |
| These Thy1-Brainbow 1.0 (line L) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Prior to Cre-mediated recombination, the fluorescent protein immediately adjacent to the promoter, dTomato (RFP), is expressed in peripheral and central neurons. When bred to Cre recombinase expressing mice, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): dTomato (RFP) (no recombination), mCerulean (CFP), or mYFP. Integration of tandem transgene copies yields combinatorial fluorescent protein expression in each cell, and thus many possible cell colors, providing a way to distinguish adjacent neurons and visualize other cellular interactions. Of note, the singl ..... For more information please see the full phenotype on the strain data sheet | ||
| 011070 | B6;CBA-Tg(Thy1-EGFP)SJrs/NdivJ | Repository- Live |
| Mice harboring the Thy1-GFP transgene are viable and fertile with enhanced green fluorescent protein (EGFP) expression under the control of a modified Thy1 promoter region (containing the sequences required for neuronal expression but lacking the sequences required for expression in non-neural cells). These thy1-GFP-S mice (derived from founder line S) have EGFP expression in sparse subsets of neurons within specific populations; providing a bright, vital Golgi-like stain. Thy1-GFP-S mice show EGFP labeling in the superficial layers of the neocortex, including most/all interneuron subtypes, with pyramidal/interneuron ratios of approximately 4:1 that match the distribution in the non-labeled population. While this is similar to Thy1-GFPM mice (Stock No. 007788), the labeling distribution for line S is different from line M. In addition, less than 10% of cerebellum mossy fibers show EGFP labeling and no EGFP expressio ..... For more information please see the full phenotype on the strain data sheet | ||
| 015814 | B6;CBA-Tg(Thy1-spH)64Vnmu/FrkJ | Repository- Live |
| These transgenic mice express spH, the pH-sensitive variant of the green fluorescent protein (ecliptic pHluorin) fused to the mouse synaptic vesicle-associated membrane protein 2 (Vamp2), under the control of the mouse thymus cell antigen 1 (Thy1) promoter. In founder line spH64, transgene expression is detected in many regions of the brain. spH expression is detected mainly in GABAergic or inhibitory synaptic terminals of dissociated hippocampal neurons. Synaptic activity, as determined by fluorescence, is detectable starting at approximately 10 days in vitro in cultured hippocampal neurons. | ||
| 013137 | B6;D2-Tg(Akr1b7-RFP)9Amc/J | Repository- Live |
| These transgenic mice express Red Fluorescent Protein (TagRFP-T variant) under the direction of the mouse Akr1b7, aldo-keto reductase family 1, member B7, promoter.
Transgene expression is detected at high levels in the adrenal glands and endothelial cells of renal medullary arterioles of transgenic embryos aged E15.5.
Renal vasculature associated expression is observed in Smooth Muscle Actin immunohistochemical positive cells that are closely associated with FLK1 and PECAM positive intrarenal arteries. Other possible sites of expression have not been characterized.
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous. This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 015853 | B6;DBA-Tg(Cited1-TagRFP)26Amc/J | Repository- Live |
| These transgenic mice express the Tag-RFPT variant of Red Fluorescent Protein under the direction of the mouse Cited1, Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 1, promoter. TagRFP is the optimized monomeric derivative of the tetrameric eqFP578 from the sea anemone, Entacmaea quadricolor.
RFP fluorescence is detected in the cap mesenchyme in the kidney and the male reproductive systems of hemizygous embryos, 15.5 embryonic days of age. Immunohistochemical analysis reveals that the TagRFP-T transgene is expressed in the expected Cited1 expression domain. Other possible sites of expression have not been characterized. Mice that are hemizygous for the knock in allele are viable, fertile, normal in size and do not display physical or behavioral abnormalities.
This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 008344 | B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J | Repository- Live |
| The TetTag mouse is a bi-transgenic mutant that has tetracycline (or tetracycline analog) inducible expression of beta-galactosidase in activated neurons. Two independently generated transgenic strains were crossed to produce this bi-transgenic TetTag strain. In the first transgenic construct, the tetracycline-controlled transactivator (tTA) protein and a two hour half-life Green Fluorescent Protein (shEGFP) are expressed under the direction of the fos, FBJ osteosarcoma oncogene, minimal promoter. The second transgenic construct expresses a nuclear-localizing beta-galactosidase gene and the doxycycline insensitive tetracycline regulated transactivator (containing point mutation, H100Y), under the control of the tetO, tetracycline-responsive regulatory element. In the absence of tetracycline or a tetracycline analog (such as doxycycline), selective expression of beta-galactosidase is observed in activated neurons. Doxycycline administration prevents expression beta-galactosidase ..... For more information please see the full phenotype on the strain data sheet | ||
| 014160 | B6;DBA-Tg(S100b-EGFP/cre/ERT2)22Amc/J | Repository- Live |
| These transgenic mice express the eGFPCreERT2 (Enhanced Green Fluorescent Protein and cre/ESR1) fusion gene under the direction of the mouse S100b, S100 protein, beta polypeptide, neural, promoter. Transgene expression is detected in chondrocytes in developing bone and in neural cells in the dorsal root ganglia (DRG). GFP fluorescence is not detectable by fluorescent microscope examination of whole embryos, bones or neural tube sagittal slices from embryos aged 15.5dpc. Tamoxifen induced Cre recombinase activity is detected in a subset of the GFP immunoreactive-positive cells in bone and to a lesser extent in the dorsal root ganglia. GFP immunoreactive-positive cells co-localize with S100b immunoreactive-positive cells in bone and DRG. Other possible sites of expression have not been characterized. Mice that are hemizygous for the transgene are viable, fertile, normal in size and do not display physical or behavioral abnormalities. This strain was transfe ..... For more information please see the full phenotype on the strain data sheet | ||
| 014159 | B6;DBA-Tg(Tmem100-EGFP/cre/ERT2)30Amc/J | Repository- Live |
| These transgenic mice express the eGFPCreERT2 (Enhanced Green Fluorescent Protein and cre/ESR1) fusion gene under the direction of the mouse Tmem100, transmembrane protein 100, promoter. Transgene expression is detected in developing nephrons of the kidney, Wolfian and Mullerian ducts and vasculature of the urogenital system of hemizygous 15.5 dpc embryos. GFP fluorescence is detected in embryos 15.5dpc in age in the proximal region of the early renal vesicle, at later stages eGFP is present in the parietal epithelium adjacent to Wilms Tumor (WT1) positive cells demarcating the future podocytes of Bowman's capsule and is limited to the Tmem100 expression domain. Tamoxifen inducible Cre recombinase activity is detected in the developing kidney (nephrogenic zone), vasculature and Wolfian or Mullerian duct of mutant embryos aged 15.5dpc. Possible cre recombinase activity is detected in small renal arteries. Other possible sites of expression have not been char ..... For more information please see the full phenotype on the strain data sheet | ||
| 010803 | B6;FVB-Tg(Adipoq-cre)1Evdr/J | Repository- Live |
| Mice hemizygous for this Adipoq-Cre BAC transgene are viable and fertile, with expression of a Cre recombinase directed to adipose tissue by the promoter/regulatory regions of the mouse adiponectin (Adipoq) locus on the BAC transgene. Transcription/translation from the BAC Adipoq locus is disabled, and Cre recombinase expression levels are similar to that of endogenous Adipoq expression. These mice express Cre recombinase effectively in white adipose tissue (WAT) and brown adipose tissue (BAT), but not in macrophages (including adipose-tissue resident macrophages, alveolar macrophages, or thioglycollate-stimulated peritoneal macrophages). The donating investigator reports highly efficient Cre recombinase activity, with no ectopic expression. The phenotype of homozygous mice was not determined by the donating investigator. These Adipoq-Cre BAC transgenic mice may be useful in generating conditional mutations for studying adipose tissue function and storage, obesity, ..... For more information please see the full phenotype on the strain data sheet | ||
| 009159 | B6;FVB-Tg(Cnp-EGFP/Rpl10a)JD368Htz/J | Repository- Live |
| These BAC TRAP transgenic mice express the EGFP-L10a fusion gene, EGFP/Rpl10a, under the control of the mouse Cnp (2',3'-cyclic nucleotide 3' phosphodiesterase) promoter. The transgene expression pattern corresponds to endogenous Cnp expression. Green fluorescent protein is detected in mature oligodendrocytes. Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. Transgenic male mice are infertile. Attempts at cryopreserving sperm from male transgenic mice have failed. This mutant mouse strain may be useful in affinity purification of polysomal mRNAs (translating ribosome affinity purification or TRAP) from mature oligodendrocytes and tracking mature oligodendrocytes by fluorescence. | ||
| 003800 | B6;SJL-Tg(ACTFLPe)9205Dym/J | Repository- Live |
| This transgenic strain expresses a variant of the Saccharomyces cerevisiae FLP1 recombinase gene under the direction of the human ACTB promoter. The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being four-fold and ten-fold that of wildtype FLP at 37C and 40C, respectively. Mice that are hemizygous for the transgenic allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5. This deleter strain is a suitable alternative to, and complement with the Cre-loxP system for in vivo genetic engineering. | ||
| 005249 | B6;SJL-Tg(Krt1-15-cre/PGR)22Cot/J | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice have a synthetic steroid RU 486 inducible Cre-mediated recombination system driven by the mouse keratin complex 1, acidic, gene 15 promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the human progesterone receptor. The mutant human progesterone receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, RU 486. Restricted to the cytoplasm, the Cre/PGR protein can only gain access to the nuclear compartment after exposure to RU 486. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating RU 486-induced, Cre-mediated targeted deletions. This strain represents an effective tool for generating tissue-specific targeted mutants that would be useful to study epi ..... For more information please see the full phenotype on the strain data sheet | ||
| 010576 | B6;SJL-Tg(MMTV-rtTA)4-1Jek/J | Repository- Live |
| The donating investigator claims homozygous mice are viable and fertile. These MMTV-rtTA mice have expression of the reverse tetracycline-controlled transactivator (rtTA) protein directed primarily to the breast epithelia of the mammary ductal system by the mouse mammary tumor virus (MMTV) promoter. The donating investigator also reports some rtTA expression in salivary glands (particularly in the males) as well as prostate glands. When mated to a mutant strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE, TetRE or tetO), expression of the target gene may be induced with administration of the tetracycline analog doxycycline (dox) in the double mutant offspring. These MMTV-rtTA mice are a Tet-On tool that allows conditional, dox-inducible expression of genes primarily in mammary gland epithelial cells and may be useful in studying the endocrine function of mammary tissues and/or breast cancer (for example). | ||
| 008850 | B6;SJL-Tg(Mt1-LDLR)93-4Reh/AgnJ | Repository- Live |
| Mice hemizygous for this MMT-I-hLDLR transgene (hLDLRTg mice) are viable and fertile, with human low density lipoprotein receptor (hLDLR) expression controlled by the mouse metallothionein 1 gene (Mt1) promoter sequences. The donating investigator reports that homozygous mice are not viable. Expression of hLDLR mRNA is highest in liver, moderate in kidney, small intestine, and heart, and lowest in brain and pancreas. Treatment with cadmium sulfate (CdSO4) stimulates transcription from the metallothionein promoter and results in higher levels of hLDLR expression. This overexpression of functional LDLR in transgenic mice results in greatly increased clearance of LDLR ligands (LDL, apoprotein B-100 and apoprotein E) from plasma when compared to wild-type mice. These hLDLRTg mice may be useful for studying the role of apolipoproteins and their receptors, lipid transport and lipoprotein metabolism in a wide variety of disease (including atherosclerosis, Alzheimer's disease, and hepat ..... For more information please see the full phenotype on the strain data sheet | ||
| 012355 | B6;SJL-Tg(Pvalb-COP4*H134R/EYFP)15Gfng/J | Repository- Live |
| Mice hemizygous for the Prv-mhChR2-YFP BAC transgene are viable and fertile with expression of the mhChR2::YFP fusion protein directed to neuronal populations by the mouse parvalbumin (Pvalb or Prv) promoter/enhancer regions on the BAC transgene. The mhChR2::YFP fusion protein is composed of a mammalian codon-optimized Chlamydomonas reinhardtii-derived channelrhodopsin-2 that was modified to harbor a gain-of-function H134R substitution (mhChR2; also called hChR2-H134R) fused in-frame with an enhanced yellow fluorescent protein (EYFP). The mhChR2 is designed to cause larger stationary photocurrents compared to ChR2. The bacterial opsins are retinal-binding proteins that provide light-dependent ion transport and sensory functions to a family of halophilic bacteria; and this mhChR2 functions as a blue light-driven cation channel that depolarizes the cell and causes action potentials. As such, illuminating mhChR2-expressing neurons with blue light (450-490 nm) leads to rapid ..... For more information please see the full phenotype on the strain data sheet | ||
| 013094 | B6;SJL-Tg(Sox10-cre)507Mcln/J | Repository- Live |
| Mice hemizygous for the S4F:cre transgene are viable and fertile, containing the SRY-box containing gene 10 (Sox10) promoter and a c-Fos minimal promoter sequence directing expression of Cre recombinase predominantly to neural crest derived cells. Specifically, Cre recombinase expression is observed in craniofacial skeletal components, sympathetic and parasympathetic neuronal/glial populations as well as epidermal melanocyte precursors. Expression is also evident in non-neural crest derived tissues including oligodendrocytes and the ventral neural tube. When these transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. These mice may be important for lineage tracing, gene function characterization, and genome manipulations. | ||
| 008134 | B6;SJL-Tg(THY1-SNCA*A30P)TS2Sud/J | Repository- Live |
| The A30P mutation in this transgenic strain is associated with the development of familial Parkinson's disease. The onset of hind limb mobility problems occurs around 12 months of age (sometimes earlier), induced by a loss of motor neurons and associated with the formation of insoluble alpha synuclein aggregates. This strain may be useful in studies of Parkinson's disease. Hemizygous mice are viable and fertile. | ||
| 012348 | B6;SJL-Tg(Thy1-COP3/EYFP)8Gfng/J | Repository- Live |
| Mice hemizygous for the Thy1-VChR1-YFP transgene are viable and fertile with expression of the VChR1::YFP fusion protein directed to neural cells by the modified murine Thy1 promoter region. The VChR1::YFP fusion protein is composed of a synthetic, mammalian codon-optimized, red-shifted channelrhodopsin-1 derived from Volvox carteri (VChR1) fused in-frame with an enhanced yellow fluorescent protein (EYFP). Compared with ChR2, VChR1 has a markedly (~70 nm) red-shifted action spectrum with a maximum at ~535 nm (green light). The bacterial opsins are retinal-binding proteins that provide light-dependent ion transport and sensory functions to a family of halophilic bacteria; and this VChR1 functions as a green light-driven cation channel that depolarizes the cell and causes action potentials. As such, illumination of VChR1-expressing neurons leads to rapid and reversible photostimulation of action potential firing/neural activity in these cells. The donating investigator spec ..... For more information please see the full phenotype on the strain data sheet | ||
| 012350 | B6;SJL-Tg(Thy1-COP4*H134R/EYFP)20Gfng/J | Repository- Live |
| Mice hemizygous for the Thy1-mhChR2-YFP transgene are viable and fertile with expression of the mhChR2::YFP fusion protein directed to neural cells by the modified murine Thy1 promoter region. The mhChR2::YFP fusion protein is composed of a mammalian codon-optimized Chlamydomonas reinhardtii-derived channelrhodopsin-2 (optimized with an N-terminal beta2 nictinic acytlcholine receptor signal peptide and C-terminal ER-export and Golgi-export motifs) that harbors a gain-of-function H134R substitution fused in-frame with an enhanced yellow fluorescent protein (EYFP). The mhChR2 is designed to cause larger stationary photocurrents compared to ChR2. The bacterial opsins are retinal-binding proteins that provide light-dependent ion transport and sensory functions to a family of halophilic bacteria; and this mhChR2 functions as a blue light-driven cation channel that depolarizes the cell and causes action potentials. As such, illuminating mhChR2-expressing neurons with blue light ..... For more information please see the full phenotype on the strain data sheet | ||
| 012332 | B6;SJL-Tg(Thy1-HOP/EYFP)2Gfng/J | Repository- Live |
| Mice hemizygous for the Thy1-eNpHR-YFP transgene are viable and fertile with expression of the eNpHR::YFP fusion protein directed to neural cells by the modified murine Thy1 promoter region. The donating investigator specifically reports that Thy1-eNpHR-YFP mice derived from founder line 2 exhibit high expression of EYFP in layer 5 pyramidal neurons at cortex, dentate gyrus, thalamus, superior colliculus, inferior culliculus, brainstem, amydagala and cerebellum. These Thy1-eNpHR-YFP line 2 transgenic mice may be useful for rapid control of motor behavior by addition or removal of light, for ex vivo and in vivo studies of neural circuitry/connectivity following illumination, or for fluorescent labeling of neural tissues.
The eNpHR::YFP fusion protein, designed with halorhodopsin from the halophilic bacterium Natronomonas pharaonis (NpHR) fused in-frame with an enhanced yellow fluorescent protein (EYFP), was optimized for expression in mammalian systems ..... | ||
| 012334 | B6;SJL-Tg(Thy1-HOP/EYFP)4Gfng/J | Repository- Live |
| Mice hemizygous for the Thy1-eNpHR-YFP transgene are viable and fertile with expression of the eNpHR::YFP fusion protein directed to neural cells by the modified murine Thy1 promoter region. The donating investigator specifically reports that Thy1-eNpHR-YFP mice derived from founder line 4 exhibit high EGFP expression in layer 2/3 and layer 5 pyramidal neurons at cortex, dentate gyrus, thalamus, superior colliculus, inferior culliculus, brainstem, amydagala and cerebellum. These Thy1-eNpHR-YFP line 4 transgenic mice may be useful for rapid control of motor behavior by addition or removal of light, for ex vivo and in vivo studies of neural circuitry/connectivity following illumination, or for fluorescent labeling of neural tissues.
The eNpHR::YFP fusion protein, designed with halorhodopsin from the halophilic bacterium Natronomonas pharaonis (NpHR) fused in-frame with an enhanced yellow fluorescent protein (EYFP), was optimized for expression in mammali ..... | ||
| 007610 | B6;SJL-Tg(Thy1-cre/ERT2,-EYFP)VGfng/J | Repository- Live |
| These transgenic mice ("SLICK" for single-neuron labeling with inducible Cre-mediated knock-out) have a tamoxifen inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted neuron-specific deletions only in neurons where the Thy1 is active. These cells will be evident by the expression of Yellow Fluorescent Protein. Transgenic mice express YFP constitutively in neurons. The Donating Investigator reports that founder line V mice express the transgene sparsely in neurons of the central nervous system.
This strain is one of ..... | ||
| 014555 | B6;SJL-Tg(Tph2-COP4*H134R/EYFP)5Gfng/J | Repository- Live |
| Mice hemizygous for the TpH2-mhChR2-YFP BAC transgene (or TpH2-ChR2-YFP BAC transgene) are viable and fertile, with expression of the mhChR2::YFP fusion protein directed to serotonergic neuronal populations by the mouse tryptophan hydroxylase 2 (Tph2 or TpH2) promoter/enhancer regions on the BAC transgene. The mhChR2::YFP fusion protein is composed of a mammalian codon-optimized Chlamydomonas reinhardtii-derived channelrhodopsin-2 that was modified to harbor a gain-of-function H134R substitution (mhChR2; also called hChR2-H134R) fused in-frame with an enhanced yellow fluorescent protein (EYFP). The mhChR2 is designed to cause larger stationary photocurrents compared to ChR2. The bacterial opsins are retinal-binding proteins that provide light-dependent ion transport and sensory functions to a family of halophilic bacteria; and this mhChR2 functions as a blue light-driven cation channel that depolarizes the cell and causes action potentials. As such, illuminating mhChR2-ex ..... For more information please see the full phenotype on the strain data sheet | ||
| 010577 | B6;SJL-Tg(tetO-Erbb2*)8-4Jek/J | Repository- Live |
| The donating investigator reports that homozygous mice are viable and fertile. These TetRE-ErbB2 (TRE-ErbB2 or TRE-Neu) transgenic mice have expression of an activated form of the rat ErbB2 regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter. When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), expression of the constitutively active ErbB2 protein may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring.
The amino acid mutation within the transmembrane domain of the ErbB2 receptor protein kinase facilitates its oligomerization and activation independent of a ligand; resulting in hyperplasia in tissues where it is expressed.
These TetRE-ErbB2 mice may be bred to generate bi-transgenic mutant mice with conditional (inducible/r ..... For more information please see the full phenotype on the strain data sheet | ||
| 003627 | B6C3-Tg(HD82Gln)81Dbo/J | Repository- Live |
| Mice expressing this transgene appear normal at birth through 1-2 months. Mice fail to gain weight, develop tremors, hypokinesis and lack coordination. They exhibit an abnormal gait and frequent hind limb clasping. Life expectancy is 5-6 months. Studies using huntingtin antibodies indicated numerous immunoreactive nuclear inclusions in multiple neuron populations. Neuritic damage is evident. | ||
| 014170 | B6N.Cg-Tg(UGT1A1*28)1Rhtu/J | Repository- Live |
| Transgenic UGT1A1*28 mice carry the entire human uridine diphosphate (UDP) glucuronosyltransferase 1 (UGT1) locus, and includes a mutant form of the human UGT1 polypeptide A1 (UGT1A1) promoter. Hemizygous mice are viable, fertile, and normal in size. The UGT1 locus encodes a family of genes, including UGT1A1-UGT1A10. UGT1 contains a series of divergent exon 1 sequences, each encoding the substrate binding site of a different UGT1A protein, and exons 2-5 which encode the highly conserved carboxyl terminal. Each exon 1 is regulated by its own promoter/enhancer sequences. The UGT1A1*28 mutation is associated with hepatic dysfunction and increased bilirubin found in Gilbert's syndrome. UGTs detoxify small lipophilic molecules and transform them into hydrophilic glucuronides, facilitating excretion. UGT1A1*28 transgenic mice express human UGT1A genes in patterns similar to the human tissues, mainly in tissues such as ..... For more information please see the full phenotype on the strain data sheet | ||
| 002297 | B6SJL-Tg(SOD1)2Gur/J | Repository- Live |
| This transgenic strain carries the normal allele of the human SOD1 gene. Originally published as N1029, it has been reported that the SOD1 protein level is the same as in the transgenic strain carrying the SOD1*G93A transgene (002726), even though the copy number in the SOD1*G93A transgenic is higher. This strain serves as a control for the B6SJL-Tg(SOD1*G93A)1Gur/J (002726) and the B6SJL-Tg(SOD1*G93A)dl1Gur/J (002300) strains. | ||
| 003465 | BALB/c-Tg(CMV-cre)1Cgn/J | Repository- Live |
| In this transgenic strain, deletion of loxP-flanked genes occurs in all tissues, including germ cells. The cre gene in this strain is under the transcriptional control of a human cytomegalovirus minimal promoter and is likely to be expressed before implantation during early embryogenesis. It also appears that the cre gene is X-linked since transgene transmission through males is restricted to female offspring. As these cre-transgenic mice are on a BALB/c background, they are ideally suited for breeding with gene-targeted mutant mice that have been created using the BALB/c-derived ES cell line BALB/c-I.
View cre expression characterization. | ||
| 012641 | BALB/c-Tg(S100a4-cre)1Egn/YunkJ | Repository- Live |
| These transgenic mice express Cre recombinase under the control of the mouse S100a4, S100 calcium binding protein A4, promoter. Cre recombinase expression is detected specifically in stromal fibroblasts of tissues such as the prostate, forestomach, mammary gland. Mice that are homozygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. | ||
| 010545 | C.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ | Repository- Live |
| These L2G85.BALB/c mice harbor the CAG-luc-eGFP L2G85 transgene. Homozygous mice are viable and fertile, with widespread expression of firefly luciferase and enhanced green fluorescent protein directed by the CAG promoter (human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter). Bioluminesence is detected in heart, spleen, muscle, pancreas, skin, thymus and bone marrow. Luciferase activity is not detected in mature erythrocytes, although low levels are detected in erythrocyte precursors and varying levels of activity in all leukocyte subsets tested. Homozygotes have no reported gross physical or behavioral abnormalities. Following luciferin injection, luciferase expression is generally greater in males than females. GFP fluorescence is detected in skin (upper epidermal layers) by fluorescence microscopy. The Donating Investigator reports that for the FVB-Tg(CAG-luc,-GFP)L2G85Chco/J (STOCK#8450) strain, no GFP fluorescence ..... For more information please see the full phenotype on the strain data sheet | ||
| 004512 | C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J | Repository- Live |
| Mice that are homozygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. Upon diphtheria toxin (DT) administration, mice harboring this transgene are transiently depleted of dendritic cell (DC) populations. All CD8+ and CD8- DC in the spleen express EGFP and are DT sensitive. Immunohistochemical and flow cytometric analysis reveals EGFP expression and DT-inducible depletion of CD11chigh DC in spleen, lymph node, lung, liver and lamina propria tissues, as well as the defined macrophage subpopulations of the alveolar, lamina propria, metallophillic and marginal zone. Rapid reduction of CD11c+ DC populations induced by intraperitoneal injection of DT persists for approximately 2 days, after which the cell population gradually recovers. While transient DC depletion was not associated with sign of illness or long-term defects, repeated DT induction is lethal to the mouse. Long term de ..... For more information please see the full phenotype on the strain data sheet | ||
| 006567 | C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ | Repository- Live |
| This transgenic mouse line with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer makes all of the tissues, with the exception of erythrocytes and hair, appear green under excitation light. The viability of homozygous mice is unknown.
Of note, it has been the experience at The Jackson Laboratory that Stock No. 006567 (C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ) demonstrates the highest proportion of GFP expressing cell populations in bone marrow, thymus, spleen and peripheral blood when compared to Stock No. 003291 (C57BL/6-Tg(CAG-EGFP)1Osb/J) and Stock No. 007075 (CByJ.B6-Tg(CAG-EGFP)1Osb/J).
View the pdf document on GFP Expressing Lymphocyte Populations in C57BL/6-T ..... | ||
| 005145 | C57BL/6-Tg(CAG-OVA)916Jen/J | Repository- Live |
| Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the membrane bound chicken ovalbumin OVA gene under the direction of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate-early enhancer. Chicken ovalbumin expression is detected by immunohistochemical analysis of all tissues. Splenocytes from transgenic mice display the 254-267-Kb complex, which is recognized by T-cells from the transgenic strain C57BL/6-Tg(TcraTcrb)1100Mjb/J (Stock No. 3831) and the 323-339-I-Ab complex, which is recognized by T-cells from the transgenic C57BL/6-Tg(TcraTcrb)425Cbn/J (Stock No. 4194). Skin grafts from transgenic mice are rejected by C57BL/6 recipients. Ovalbumin antigen specific T cells can be tracked in vivo. This mutant mouse strain represents a model that may be useful in studies of adoptive transfer and graft rejection a ..... For more information please see the full phenotype on the strain data sheet | ||
| 014176 | C57BL/6-Tg(CLEC4C-HBEGF)956Cln/J | Repository- Live |
| BDCA2-DTR transgenic mice have a simian diphtheria toxin receptor (DTR) under the transcriptional control a human C-type lectin domain family 4, member C (CLEC4C or BDCA2) promoter. Homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice express DTR specifically in plasmacytoid dendritic cells (pDCs). pDCs are bone marrow-derived leukocytes that respond to viral infection by secreting interferons and chemokines. This promotes resistance to viral infection and apoptosis of infected cells as well as recruitment and activation of natural killer (NK) cells, dendritic cells (DC), T cells, and B cells. After diphtheria toxin injection, 90% of pDCs are depleted in blood, spleen, liver, and lymph nodes. These mice may be useful for studying pDC function during the adaptive and innate immune responses to viral infection. | ||
| 013760 | C57BL/6-Tg(Camk2a-AIDPak)21Stl/J | Repository- Live |
| dnPAK transgenic mice have a mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter directing postnatal forebrain expression of the mouse dominant negative (dn) autoinhibitory domain (AID) of p21 protein (Cdc42/Rac)-activated kinase (Pak) gene (dnPAK). The AID is conserved in all three Pak genes (Pak1, Pak2, Pak3). Homozygous dnPAK mice are viable, fertile, and normal in size. Pak genes are expressed in multiple brain regions, including the cortex and hippocampus, and are critical regulators of actin remodeling. The activation of PAK catalytic activity requires the release of the catalytic domain from the AID and autophosphorylation of a gene specific threonine residue. dnPAK binds to the catalytic domain of PAK and blocks autophosphorylation, inhibiting activation of catalytic activity. dnPAK overexpression causes a decrease in the number of and increase is the size of cortical neuron dendri ..... For more information please see the full phenotype on the strain data sheet | ||
| 010712 | C57BL/6-Tg(Camk2a-tTA)1Stl/J | Repository- Live |
| When these transgenic mice are crossed with a tissue or cell-specific cre strain to remove a floxed stop cassette, tetracycline-controlled transactivator (tTA) is expressed in hippocampal CA3 and the dentate gyrus under the control of the calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter. When further mated to a transgenic strain that carries a gene of interest driven by a tetracycline-responsive promoter element (TRE; tetO), expression of that gene can be conditionally regulated by the presence or absence of doxycycline in the drinking water. Expression in tTA/tetO animals can be reversibly inhibited by the presence of doxycycline or induced by withdrawal of the tetracycline analog. | ||
| 016097 | C57BL/6-Tg(Car1-cre)5Flt/J | Repository- Live |
| These transgenic mice express Cre recombinase under the control of the mouse Car1, carbonic anhydrase 1, promoter. Transgenic transcript is detected in the tissues of the large intestine (cecum, proxmial and distal colon) by RT-PCR. Low levels of transcript are detected in the liver, and no transgene transcript is detected in kidney, stomach, bone marrow, prostate, lung, thymus, liver hear, duodenum, jejunum, ileum, pancreas, spleen. Mosaic recombinase activity is detected as early as 14.5 dpc in approximately 15% of the epithelial cells of the large intestine and is observed in individual crypts from the base to lumen. When crossed with a strain containing loxP site-flanked sequences, cre-mediated recombination results in large intestine epithelial-specific deletion of the flanked sequences in the offspring. Mice that are homozygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. | ||
| 011086 | C57BL/6-Tg(Cck-cre)CKres/J | Repository- Live |
| A minimal Cck (cholecystokinin) promoter drives expression of Cre in this transgenic strain. These mice express Cre at high levels in the brain cortex in a pattern that is qualitatively similar to that of the wildtype Cck gene. There is virtually no expression in subcortical regions. Although a component of the transgene, DsRed2 is not expressed. This strain may be useful for various psychiatric and neuroscience studies of this neuropeptide promoter. | ||
| 014639 | C57BL/6-Tg(Cd4-TcraDN32D3)1Aben/J | Repository- Live |
| Vα14-Jα18 transgenic mice contain CD4 promoter/enhancer elements driving expression of prearranged Vα14-Jα18 T cell receptor (TCR) cDNA from hybridoma line DN32.D3. Hemizygous mice are viable and fertile. These mice overexpress Natural Killer T (NKT) cells, or Vα14 NKT cells, in the liver, thymus, spleen, and lymph nodes. These mice may be useful for studying positive/negative selection, T cell receptor interactions, and NKT cell function. | ||
| 014644 | C57BL/6-Tg(Cd4-Zbtb16)1797Aben/J | Repository- Live |
| CD4-PLZF transgenic mice contain CD4 promoter/enhancer elements driving expression of the zinc finger and BTB domain containing 16 (Zbtb16 or PLZF) cDNA from purified natural killer T (NKT cells). Hemizygous mice are viable and fertile. PLZF is a transcriptional regulator that is expressed in NKT cells and is required for their maturation into effector-type lymphocytes. Transgenic expression of PLZF using the CD4 promoter induces
conventional CD4 T cells to acquire effector characteristics. While
CD4 T cells are present at normal levels in the thymus, spleen, liver,
and lungs of the CD4-PLZF mouse, they are nearly absent in the blood
and lymph nodes. Greater than 98% of mature CD4 T cells display a
CD44hi/CD62Llo effector phenotype. An increased percentage of CD4 T cells produce both interleukin-4 (IL-4) and interferon (IFN) γ upon TCR stimulation. These mice may be useful for studying the function
of PLZ ..... For more information please see the full phenotype on the strain data sheet | ||
| 008766 | C57BL/6-Tg(Cd8a-cre)1Itan/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is observed in peripheral CD8+ T cells (CD8 α+CD8β+ αβT cells and CD8α+CD8β- αβT cells, but not in CD4+CD8α-CD8β- αβT cells). When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked genome in cells that normally express Cd8a. | ||
| 005070 | C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J | Repository- Live |
| These Macrophage Fas-Induced Apoptosis (MAFIA) transgenic mice have an inducible Fas suicide/ apoptotic system driven by the mouse Csf1r, colony stimulating factor 1 receptor promoter. The transgene insert contains a mutant human FK506 binding protein 1A, 12kDa (FKBP12) which preferentially binds the dimerization drug AP20187. Enhanced Green Fluorescent Protein (EGFP) fluorescence and transgene expression is detected in 78% of isolated peritoneal cells. EGFP fluorescence is variable among tissues (B- and T-cells do not express EGFP). Administration of the dimerizing reagent, AP20187, induces apoptosis in macrophages and dendritic cells (intravenous injection of dimerizer is recommended, since the intraperitoneal route can elicit peritoneal adhesions). In treated mice, EGFP fluorescing peritoneal and bone marrow macrophage numbers are depleted by more than 90%, and macrophage numbers in blood, spleen, lung and thymus by more than 70%. 7 days after cessation of treatment, the EGFP ..... For more information please see the full phenotype on the strain data sheet | ||
| 016133 | C57BL/6-Tg(Defa2-Myd88)1Lvh/J | Repository- Live |
| The mouse Defa2 (defensin, alpha, 2; also known as cryptdin 2 or CR2) promoter directs expression of FLAG-tagged Myd88 (myeloid differentiation primary response gene 88) to the Paneth cells of the small intestine in this transgenic strain. Hemizygotes are viable and fertile and do not display any gross physical or behavioral abnormalities. Anti-FLAG antibodies may be used for immunohistochemical analysis. This strain may be useful in studies of microbial/immune system interactions and antimicrobial transcriptional responses at the intestinal epithelial barrier. | ||
| 006474 | C57BL/6-Tg(Grik4-cre)G32-4Stl/J | Repository- Live |
| Mice hemizygous for this "G32-4" transgene are viable, fertile, and do not display any gross physical or behavioral abnormalities. Transgene expression (cre activity) is detectable at 14 days old in area CA3 of the hippocampus, and at 8 weeks of age, recombination is observed in nearly 100% of pyramidal cells in area CA3. Recombination is also observed in other brain areas, but at distinctly lower frequencies. If bred with mice containing a loxP-flanked sequence of interest, tissue-specific deletion of that sequence results in the offspring. Specifically, when these mice were bred with a conditional CaMKII allele (see Stock No. 006575), the resulting offspring exhibited altered neurotransmitter release. The donating investigator reports that G32-4 females may confer global Cre-mediated deletion of loxP-flanked sequences in some offspring, and recommend using male G32-4 mice exclusively for such trials. These C ..... For more information please see the full phenotype on the strain data sheet | ||
| 011005 | C57BL/6-Tg(H2-Kb-Tcra,-Tcrb)P25Ktk/J | Repository- Live |
| These P25 TCR-Tg mice contain CD4 + T cells expressing a transgenic T-cell antigen receptor that recognizes peptide 25 (aa 240-254) of Mycobacterium tuberculosis Antigen 85B bound to I-Ab. Homozygotes are viable, fertile, and normal in size. These transgenic mice, with T cell response specific to M. tuberculosis infection, may be useful for studying the process of the adaptive immune response to M. tuberculosis infection in the lungs. These mice exhibit an immune response that is not dependant on bacteria number in the tissue, but rather on movement of bacterium into the draining nodes of the lungs. | ||
| 002595 | C57BL/6-Tg(IghelMD4)4Ccg/J | Repository- Live |
| Cells from mice carrying the IghelMD4 transgene recognize hen egg lysozyme. More than 90% of B-cells in the spleen are derived from the transgene (a allotype) and are predominantly IgM and IgD. This strain may be used to study B-cell selection. | ||
| 005431 | C57BL/6-Tg(Ins2-TFRC/OVA)296Wehi/WehiJ | Repository- Live |
| Ins2-TFRC/OVA (commonly referred to as RIP-mOVA) line 296-1B hemizygote transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Immunohistochemical analysis detects strong expression in pancreatic beta cells and kidney proximal tubular cells and weak expression in testes. When C57BL/6-Tg(Ins2-TFRC/OVA)296Wehi/WehiJ is mated to C57BL/6-Tg(TcraTcrb)1100Mjb/J (commonly referred to as OT-1 and recognizes OVA specific T-cells, Stock No. 003831) thymic deletion of OT-1 cells is observed in double transgenic mice, suggesting very low levels of thymic expression. OVA specific CD-8+ T-cells activated by cross presentation infiltrate the pancreas causing beta cell destruction resulting in diabetes. However, these cells do not infiltrate the kidney. Proliferating OT-1 T-cells appeared specifically in the lymph nodes draining the pancreas (PLN's) and kidney (RLN's) on day ..... | ||
| 012943 | C57BL/6-Tg(Ins2-luc/EGFP/TK)300Kauf/J | Repository- Live |
| C57BL/6J mice carrying tri-fusion transgene Ins2-luc/EGFP/Tk, commonly called MIP-TF, line 300 express a luciferase/enhanced green florescent protein/thymidine kinase fusion protein (luc/EGFP/Tk) under the control of mouse insulin promoter (Ins2) and are viable, fertile and exhibit no gross physical or behavioral abnormalities. Transgene expression, determined by luciferase enzymatic activity and green florescence is exclusive to the pancreas, specifically the insulin producing cells. Transgene expression appears to have no effect on weight, islet morphology, fasting blood glucose, or response to glucose challenge when compared to wild-type controls. This trifusion reporter model should enable longitudinal noninvasive imaging of beta cells in the same animal by cooled charge coupled device (CCD) and micro positron emission tomography (microPET), and identification of beta cells at the cellular level by florescent microscopy. There is a correlation between CCD and microPET signals fr ..... For more information please see the full phenotype on the strain data sheet | ||
| 012906 | C57BL/6-Tg(Nes-cre/Esr1*)1Kuan/J | Repository- Live |
| Mice homozygous for the nestin-CreER transgene are viable and fertile, with the rat nestin enhancer/hsp68 minimal promoter directing expression of a tamoxifen-inducible Cre recombinase (Cre-ERT1). This Cre-ERT1 fusion protein is estrogen insensitive, and is only active when it binds the estrogen analog 4-hydroxytamoxifen (OHT or tamoxifen). Following tamoxifen administration, Cre recombinase activity is reported in adult neural progenitors in the subventricular zone (SVZ), perinatal SVZ glioblasts, embryonic neural progenitors, and postnatally transformed radial glial cells. When these nestin-CreER transgenic mice are bred with mice containing a loxP-flanked sequence, tamoxifen-inducible Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. These mice may be useful for studying embryonic and adult neurogenesis.
The Cre-ERT1 fusion protein consists of Cre recombinase f ..... | ||
| 008831 | C57BL/6-Tg(Neurod2-Smo*A1)199Jols/J | Repository- Live |
| These Smo/Smo transgenic mice express the constitutively active point mutation, SmoA1, of the mouse smoothened homolog (Drosophila) (Smo) gene, under the control of the 1kb mouse neurogenic differentiation 2 (Neurod2) promoter. Transgene expression is specific to cerebellar granule cells, as observed by His tag staining. Subclinical tumor incidence in mice homozygous for the transgene is 85% by one month of age and 94% by two months of age, with an average age of onset of clinical tumor symptoms at four months. Symptoms associated with advanced tumors include enlarged posterior fossa, and/or tilted head, and hunched posture. Weight loss and an ungroomed appearance are also common. The disease progresses to leptomeningeal metastasis of the brain and spine. Once clinical symptoms are noted, survival is one to two weeks. This mutant mouse strain may be useful in studies of medulloblastoma and metastasis. | ||
| 013148 | C57BL/6-Tg(Pdgfra-cre)1Clc/J | Repository- Live |
| Hemizygous Pdgfra-cre mice are viable and fertile, with cre expression directed to retinal Muller glial cells by the mouse Pdgfra (platelet derived growth factor receptor, alpha polypeptide) promoter. Expression is predominantly in the cell bodies of the inner nuclear layer (INL) of the retina, but some expression may be observed in the outer nuclear layer (ONL) and in the ganglion cell layer (GCL). The donating investigator indicates that although not examined, cre may also be active in many types of central nervous system glial cells. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. | ||
| 008535 | C57BL/6-Tg(Pf4-cre)Q3Rsko/J | Repository- Live |
| These transgenic mice express a codon-improved Cre recombinase (iCre) under the control of the mouse Pf4 (platelet factor 4), or Cxcl4, promoter. Cre recombinase expression is detected in the majority of megakaryocytes. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The BAC clone used to generate this strain also contains 4 other genes: Cxcl5, Cxcl7, Cxcl15 and Cxcl3. The additional copy of these chemokine genes in the BAC has no effect on blood count of the mutant mice. This strain represents an effective tool for generating megakaryocyte lineage-restricted specific-targeted mutants. | ||
| 003135 | C57BL/6-Tg(TRAMP)8247Ng/J | Repository- Live |
| These mice harbor the Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP); PB-Tag Line 8247 transgene. Mice hemizygous for the TRAMP transgene develop progressive forms of prostate cancer with distant site metastasis and exhibit various forms of disease from mild intraepithelial hyperplasia to large multinodular malignant neoplasia. TRAMP hemizygotes can exhibit prostatic epithelial neoplasia (PIN) by 12 weeks of age. Tumors, appearing as well differentiated adenocarcinoma, can arise by 24 weeks of age, mostly in the dorsal and lateral lobes of the prostate. By 30 weeks of age, most hemizygous mice will display evidence of metastatic spread to the lymph nodes and/or lungs, phylloides appearance of some tumors, and seminal vesicle invasion. Tumors exhibit elevated levels of nuclear TRP53 and decreased androgen receptor expression. Note that pure C57BL/6 TRAMP hemizygotes may live to 52 weeks of age or longer.
TRAMP mice are also available on a mixed C57BL/6 x FVB/N genet ..... | ||
| 006912 | C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J | Repository- Live |
| Mice hemizygous for this "2D2 TCR" (or MOG 35-55 specific TCR) transgene are viable and fertile. The myelin oligodendrocyte glycoprotein (MOG)-specific transgenic T cells are not deleted nor tolerized and are functionally competent. The majority of thymocytes in 2D2 TCR mice express high and intermediate levels of the transgenic T cell receptor (TCR), indicating efficient positive selection of transgenic T cells. The majority of CD4+ splenocytes express the transgenic TCR (as defined by Valpha3.2 and Vbeta11 expression). Cultured splenocytes are responsive to whole myelin oligodendrocyte glycoprotein (MOG) and to MOG 35-55 peptide, but not to ovalbumin (OVA) control peptides. From between 2.5 to 5 months of age, 4% of 2D2 TCR mice develop spontaneous experimental autoimmune encephalomyelitis (EAE), while within the first year 40% of 2D2 TCR mice develop spontaneous, isolated optic neuritis with neither clinical nor histological evidence of EAE. Standard EAE induction protoco ..... For more information please see the full phenotype on the strain data sheet | ||
| 012769 | C57BL/6-Tg(Thy1-Sncg)HvP36Putt/J | Repository- Live |
| Thy1mγSN transgenic mice have the mouse thymus cell antigen 1 (Thy-1) promoter directing expression of the mouse γ-synuclein (Sncg) gene. Homozygous Thy1mγSN mice are viable, fertile, and normal in size. They develop a clasping reflex, abnormal posture and gait, and other symptoms of motor dysfunction around 6 months of age, develop limb paralysis by 9 months of age, and die by 16 months of age. Homozygotes also show a 7-fold increase of γ-synuclein mRNA in neurons compared with wild-type mice, leading to motor neuron impairment and premature death. Hemizygous mice develop this phenotype starting at 12 months of age. γ-synuclein aggregates form fibrils, much like α-synuclein, aggregates of which have been associated with neurodegeneration in diseases such as Parkinson's disease. These mice may be useful for studying the pathophysiology of Parkinson's disease, and other neurodegenerative disorders. | ||
| 003651 | C57BL/6-Tg(Zp3-cre)93Knw/J | Repository- Live |
| This is a transgenic line in which cre expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene. This promoter normally directs expression exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain would be useful for deleting a floxed sequence specifically in the female germ line. The Donating Investigator suggests to accomplish this, females homozygous or heterozygous for the floxed allele, as well as hemizygous for the Zp3cre allele are crossed with wild type males. Progeny will carry the deleted-floxed allele. | ||
| 013729 | C57BL/6-Tg(tetO-EDN1,-lacZ)9Mhus/J | Repository- Live |
| These ET+ transgenic mice express the human endothelin (EDN1 or ET-1) gene and a β-galactosidase (lacZ) reporter, both regulated by the bidirectional tetracycline operator, tetO. Homozygous mice are viable, fertile, and normal in size. ET-1 is a vasoconstrictor expressed in endothelial cells, and is a key mediator in vascular tone and renal homeostatsis. When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), LacZ and ET-1 expression may be regulated with the tetracycline analog doxycycline (DOX) in the double mutant offspring.
When bred to a strain expressing tTA driven by α-myosin heavy chain (α-MHC) cardiomyocyte specific (see Stock No. 003170) overexpression of lacZ and ET-1 results in left ventricular dilation, contractile dysfunction, ca ..... | ||
| 010713 | C57BL/6-Tg(tetO-GFP/tetX)5696Stl/J | Repository- Live |
| These transgenic mice express tetanus toxin (tetX) under the control of a tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator), and may be useful in generating bi-transgenic mutant mice for the reversible, inducible inhibition of synaptic transmission. Although GFP is fused to tetX, fluorescence may not be detectable. | ||
| 013728 | C57BL/6-Tg(tetO-NOS2,-lacZ)240iMhus/J | Repository- Live |
| These iNOSβgal transgenic mice express the human nitric oxide synthase 2, inducible (NOS2 or iNOS) gene and a β-galactosidase (lacZ) reporter, both regulated by the bidirectional tetracycline operator, tetO. Homozygous mice are viable, fertile, and normal in size. NOS isoforms reduce molecular oxygen to superoxide, reactive oxygen species, and reactive nitrogen species (all of which can cause cellular toxicity and tissue damage). When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), LacZ and iNOS expression may be regulated with the tetracycline analog doxycycline (DOX) in the double mutant offspring. When bred to a strain expressing tTA driven by α-myosin heavy chain (α-MHC), cardiomyocyte specific overexpression of lacZ and iNOS result in embryonic lethality, cardiac enlargement, cardiomyopathy, bradyarrhythmia, and sudd ..... For more information please see the full phenotype on the strain data sheet | ||
| 016181 | C57BL/6-Tg(tetO-Nr1d1)1Schb/J | Repository- Live |
| These transgenic mice express hemagglutinin-tagged Nr1d1 (nuclear receptor subfamily 1, group D, member 1) cDNA under the control of a tetracycline-responsive element (TRE; tetO)/minimal cytomegalovirus (CMV) promoter. When hemizygotes are bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled tranactivator protein (tTA) under the regulation of tissue-specific promoters, transgene expression can be conditionally regulated with doxycycline. | ||
| 006481 | C57BL/6J-Tg(ACTB-NOTCH1)1Shn/J | Repository- Live |
| Transgenic mice are viable, fertile and behaviorally normal. These "CALSL-NICD (H)" mice (or simply CALSL-NICD) reportedly carry 10-20 copies of the transgene inserted into a single genomic locus. Expression of the transgene-derived intracellular domain of human NOTCH1 is prevented by a "Lox-STOP-Lox" cassette. When transgenic mice are bred to a strain expressing Cre recombinase, the "floxed stop" cassette is excised in the resulting offspring, and human NOTCH1 expression is observed in the cre-expressing tissue(s). These transgenic mice may be useful in studying early neural progenitor cell development and apoptosis, and responses to tissue-specific Notch activation. For example, when crossed to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771), this transgenic mouse strain may be useful in studies of notch signaling during apoptotic cell death. | ||
| 009655 | C57BL/6J-Tg(Dcx-DsRed)14Qlu/J | Repository- Live |
| These transgenic mice express red fluorescent protein variant (DsRed-Express) under the direction of the mouse doublecortin promoter. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Fluorescence is first detected at embryonic day 11.5. By embryonic day 13.5 fluorescence is restricted to the CNS and mimics the endogenous doublecortin expression pattern. This mutant mouse strain may be useful in studies of neural development and visualization of developing neurons. | ||
| 007567 | C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J | Repository- Live |
| Mice hemizygous for this CD11c-Cre-GFP transgene are viable and fertile. The CD11c (Itgax) promoter directs bicistronic Cre and EGFP protein expression to dendritic cells (DCs). Expression of EGFP is expected to have equimolar expression with Cre recombinase. When bred with any mouse containing a loxP-flanked sequence of interest, the resulting offspring can have Cre-mediated recombination of the flanked sequence. These CD11c-Cre-GFP transgenic mice (as well as CD11c-Cre transgenic mice (see Stock No. 008068)) may be useful for immunological studies utilizing Cre-lox technology or fluorescent protein expression in dendritic cells. | ||
| 008661 | C57BL/6J-Tg(Nkx2-1-cre)2Sand/J | Repository- Live |
| Mice homozygous for the Nkx2.1Cre (or BAC-Nkx2.1-Cre) transgene are viable and fertile, with cre expression directed to major subgroups of brain interneuron progenitors, developing lung, thyroid, and pituitary by the Nkx2.1 promoter/enhancer regions within the BAC transgene. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. For example, when bred with beta-galactosidase reporter mice, the first detectable Cre recombinase expression in double mutant offspring is observed on embryonic day (E)10.5 in the basal telencephalon. These Nkx2.1Cre (or BAC-Nkx2.1-Cre) transgenic mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain progenitors (including future major interneurons in telencephalon and hypothalamus cells), as well as developing lung, thyroid, and pituitary. | ||
| 009593 | C57BL/6J-Tg(Pomc-EGFP)1Low/J | Repository- Live |
| Mice hemizygous for this POMC-EGFP transgene are viable and fertile, with EGFP expression directed to POMC-expressing neurons by the mouse Pomc (pro-opiomelanocortin-alpha) promoter/enhancer regions. The donating investigator reports that transcripts from the transgene encode EGFP but do not express any POMC prohormone or peptides. Direct EGFP fluorescence is observed in the arcuate nucleus of the hypothalamus (ARC), in melanotrophs/corticotrophs of the pituitary gland, and (unlike other POMC promoter-driven fluorescent mice) in a subpopulation of newly born granule neurons of the dentate gyrus of the hippocampus. EGFP expression is also observed in the nucleus of the solitary tract of the medulla. EGFP expression is downregulated as neurons mature and migrate deeper into the granule cell layer. These POMC-EGFP mice may be useful in studying neuronal signaling pathways, energy metabolism, leptin activity, obesity, seizures, depression, and epilepsy. | ||
| 008239 | C57BL/6J-Tg(Th-SNCA*A30P*A53T)39Eric/J | Repository- Live |
| These hm2α-SYN-39 mice express a doubly-mutant form of human alpha-synuclein (hα-SYN) containing the A30P and A53T human mutations associated with autosomal dominant Parkinson's disease, under the control of the rat tyrosine hydroxylase promoter. Expression of hα-SYN is detected in cell bodies, axons, and terminals of the nigrostriatal system (mRNA expression in midbrain, eye, and adrenal gland, with high levels of protein expression in the cell bodies of dopaminergic neurons in the midbrain and striatum). Hemizygous mice exhibit several Parkinson's disease-related characteristics including increased density of the dopamine transporter, impairments of the ubiquitin-proteasome system, and age-related progressive loss of locomotor activity and substantia nigra pars compacta dopaminergic neurons. The Parkinson's disease-related phenotype of hm2α-SYN-39 mice is more severe than that of the control strain (hwα-SYN-5, see Stock No. > ..... For more information please see the full phenotype on the strain data sheet | ||
| 002356 | C57BL/6J-Tg(pPGKneobpA)3Ems/J | Repository- Live |
| Homozygous neoR transgenic mice (TgN3Ems) are viable and fertile with no apparent gross phenotype. The pPGKneobpA transgene has the mouse Pgk1 promoter directing widespread (but not necessarily uniform) expression of neoR: this renders TgN3Ems mice highly G418-resistant and results in a 5-fold increase in the approximate lethal dose of G418 (~480 mg/kg) compared to wildtype mice (~103 mg/kg). Treatment with G418 lethal dose (or higher) in transgenic mice leads to rapid onset of decreased movement, respiratory arrest, and death within minutes. Compared to wildtype mouse embryonic fibroblasts (MEFs), TgN3Ems MEFs exhibit greatly diminished G418 sensitivity: wildtype MEFs show essentially no resistance to G418 while TgN3Ems MEFs survive approximately 20-fold higher G418 levels. Importantly, TgN3Ems heterozygotes and TgN3Ems homozygotes are equally resistant to G418. These TgN3Ems transgenic mice may be useful as source of G418-resistant feeder cells for ge ..... For more information please see the full phenotype on the strain data sheet | ||
| 012441 | C57BL/6J-Tg(tetO-LRRK2*G2019S)E3Cai/J | Repository- Live |
| Mice hemizygous for the human leucine-rich repeat kinase 2 mutant transgene, (LRRK2*G2019S), are viable and fertile. Expression of LRRK2*G2019S is regulated by a tetracycline operator (tetO); also called tetracycline-responsive element (TRE, TetRE) or tet-operator). When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of LRRK2*G2019S protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. LRRK2 protein, also known as Dardarin, contains multiple functional domains and may function as both an active GTPase and kinase. LRRK2 may regulate alpha-synuclein-mediated neuropathology through modulating the intracellular trafficking and accumulation of SNCA protein. The amino acid mutation in the kinase domain of LRRK2 may decrease proteasomal degradation of LRRK2. These mice may be useful for studying Parkinson's disease pathogenesis and neurodegenerati ..... For more information please see the full phenotype on the strain data sheet | ||
| 012450 | C57BL/6J-Tg(tetO-SNCA)1Cai/J | Repository- Live |
| Mice hemizygous for the human synuclein (alpha-syn) transgene, (SNCA) are viable and fertile. Expression of the transgene is regulated by the tetracycline operator (tetO); also called tetracycline-responsive element (TRE, TetRE) or tet-operator). When mated to a strain expressing tetracycline-controlled transactivator protein (tTA), expression of the SNCA protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. The formation of alpha-syn aggregates is a key step in the pathogenesis of Parkinson's disease. These aggregates cause formation of fibrillar aggregates causing various forms of cytotoxicity, including impairment of proteasomal and lysosomal activities, disruption of ER-Golgi traffic and microtubule-based transport, and dysfunction of mitochondria. SNCA mice develop age-dependent, progressive neurodegeneration. These mice may be useful for studying the Parkinson's disease pathogenesis and n ..... For more information please see the full phenotype on the strain data sheet | ||
| 016582 | C57BL/6N-Tg(Slc32a1-icre/ERT2)3Gloss/J | Repository- Live |
| These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse Slc32a1, solute carrier family 32 (GABA vesicular transporter), member 1, promoter. When crossed with a strain containing a loxP site flanked sequence, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeting events. Tamoxifen administration induces Cre recombination throughout the brain in neurons that structurally resemble interneurons. Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
This Cre-ERT2 fusion protein consists of iCre recombinase (Codon-improved Cre recombinase) fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 18278 ..... | ||
| 016583 | C57BL/6N-Tg(Slc6a3-icre/ERT2)2Gloss/J | Repository- Live |
| These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse Slc6a3, solute carrier family 6 (neurotransmitter
transporter, dopamine), member 3, promoter. When crossed with a strain containing a loxP site flanked sequence, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeting events. Tamoxifen administration induces Cre recombination in dopaminergic cells of the substantia nigra and locus coeruleus. Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
This Cre-ERT2 fusion protein consists of iCre recombinase (Codon-improved Cre recombinase) fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 1827 ..... | ||
| 008234 | CB6-Tg(CAG-EGFP/CETN2)3-4Jgg/J | Repository- Live |
| Transgenic GFP-CETN2 mice are viable and fertile, expressing an enhanced green fluorescent protein-labeled human Centrin-2 (EGFP-CETN2) under the control of the chicken beta-actin promoter (with cytomegalovirus immediate early enhancer). Distinct and uniform GFP fluorescence corresponding to the two centrioles of the centrosome are observed in every tissue examined from embryonic day 14.5 through adult, independent of cell-cycle stage. Overexpression of CETN2 from the transgene is not reported to lead to any aberrant phenotype or alter the average number of centrosomes per cell. As intracellular GFP-aggregates are observed in specific regions exclusively in the adult brain, the donating investigator cautions against the use of this model in studying the centrosome in adult brain. These GFP-CETN2 mice allow fluorescent staining of the centrioles of the centrosome, and may be useful for studying mitosis, microtubule organization, cell-cycle regulation, signal transduction, transcription ..... For more information please see the full phenotype on the strain data sheet | ||
| 007677 | CB6-Tg(Gad1-EGFP)G42Zjh/J | Repository- Live |
| Mice hemizygous for this GAD67-GFP transgene are viable and fertile. GAD67-GFP (line G42) mice selectively express enhanced green fluorescent protein (EGFP) in the calcium-binding protein parvalbumin (Pv)-expressing subclass of basket interneurons (soma, dendrites, and axons) and also in putative presynaptic boutons. Transgene expression (EGFP expression) is published as early as postnatal day 0 (P0) with high expression levels throughout postnatal development. EGFP expression remains restricted to ~50% of Pv-expressing interneurons in adulthood. The donating investigator additionally reports transgene expression as early as embryonic day 14 (E14). EGFP expression is not reported in other interneuron classes positive for somatostatin (SOM), cholecystokinin (CCK), calretinin (CR), and VIP. These GAD67-GFP (line G42) mice may be useful for fluorescent labeling of the Pv-expressing subset of GABAergic neurons, allowing reliable and efficient characterization of perisomatic innervation ..... For more information please see the full phenotype on the strain data sheet | ||
| 005307 | CBy.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ | Repository- Live |
| Transgenic mice are viable, fertile, normal in size, normoglycemic and do not display any gross physical or behavioral abnormalities.The TCR expressed from this transgene is specific for influenza virus A/PR/8 hemagglutinin (HA) in the context of the MHC class I moleculeH2-Kd. Both thymic and peripheral T-cell populations are skewed toward CD8+ cells. The majority of thymocytes and virtually all CD8+ T cells in lymph nodes express the transgenic TCR beta chain. About 40% of peripheral blood CD8+ T cells react with the HA peptide presented by H2-Kd. When mated with Tg(Ins2-HA)165Bri, double transgenic neonates have similar levels of V-beta 8 and total number of thymocytes as Tg(TcraCl4,TcrbCl4) mice however the double transgenics become spontaneously diabetic after birth and die within 10 days. This mouse is further modified with the Thy1.1 allele, rather than the alternate allele present in C57BL/10, DBA/2, and BALB/c mice. T ..... | ||
| 007075 | CByJ.B6-Tg(CAG-EGFP)1Osb/J | Repository- Live |
| This transgenic mouse line with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer makes all of the tissues, with the exception of erythrocytes and hair, appear green under excitation light. Note that mice homozygous for this transgene die within the first two weeks following birth.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available. Of note, it has been the experience at The Jackson Laboratory that Stock No. 006567 (C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ) demonstrates the highest proportion of GFP expr ..... | ||
| 007076 | CByJ.B6-Tg(UBC-GFP)30Scha/J | Repository- Live |
| These transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the direction of the human ubiqutin C promoter. Mice hemizygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice express EGFP in all tissues examined. Certain hematopoetic cell types display distinct expression levels of EGFP, allowing identification of different cells types by FACS analysis. EGFP expression is uniform within a cell type lineage and remains constant throughout development. T cells have a 2-fold higher EGFP expression than CD19+B220+ B cells or peripheral blood cells. Leukocytes and red blood cells from homozygous mice fluoresce at approximately twice the level of cells from hemizygous mice. This mutant mouse strain represents a useful tool in studies related to hematopoetic cell differentiation and in vivo tracking of leukocytes.
In an attempt to offer alleles on well-characterized ..... | ||
| 005564 | FVB(Cg)-Tg(Ins2-CALM1)26Ove Tg(Cryaa-TAg)1Ove/PneJ | Repository- Live |
| Transgenic mice are viable, display small eyes with cataracts and are hyperglycemic within 24 hours of age. S1 Nuclease protection assays indicate expression of calmodulin in the pancreas of 40-60 day old transgenic mice. Immunofluorescence analysis of the pancreas indicates increased levels of camodulin in B-cells of the pancreas. Camodulin fluorescing cells were not detected in the pancreatic acinar cells or any other tissue. Digital image scanning indicates the concentration of camodulin in transgenic B-cells is 5-fold higher than in wild type controls. Pancreatic insulin is 28% of control levels by 15 days of age. Insulin mRNA levels were also reduced in the transgenic animals. Transgenic mice experience hypoalbuminemia, elevated blood pressure, impaired cardiomyocytes and late stage diabetic nephropathy. Although transgenic mice are hyperglycemic within 24 hours of age, they are able to survive over a year without insulin treatment. This stock is useful for studying systemic c ..... | ||
| 016193 | FVB-Tg(ACTA1-PABPN1*A17)1Drub/DrubJ | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities until roughly four months of age. At four months, hemizygotes develop a progressive muscle weakness (measured by grip strength, wire maneuver and vertical gripping tests), which progresses to late onset locomoter defects around nine months of age. At nine months mice cannot lift their own body weight. They drag their pelvis when walking. There is no difference in body weight or mortality up to 15 months of age compared to controls. Hemizygotes develop KCl-insoluble inclusions containing PABPN1 in the nuclei of skeletal muscle fibers with tubulo-filamentous ultrastructures. The proportion of myocte nuclei with aggregates increases with age. Significantly elevated numbers of TUNEL-positive myocyte nuclei can be found at six and 12 months. TUNEL staining is widely used as a cell-death marker in muscle diseases in mice and humans. Muscles of hemizy ..... For more information please see the full phenotype on the strain data sheet | ||
| 013591 | FVB-Tg(C3-1-TAg)cJeg/JegJ | Repository- Live |
| Male transgenic mice develop prostatic hyperplasia in early life that progresses to adenoma or adenocarcinoma in about half of the animals which survive longer 7 months of age. Female animals generally develop mammary intraepithelial neoplasia with similarities to DCIS by 3 months of age with subsequent development of mammary adenocarcinoma by 6 months of age in 100% of the animals. About 10 - 15% of female mice develop lung metastases, although lung metastases from prostate cancer is extremely rare. Bone metastases have not been observed. The phenotype for this transgene has been most extensively studied in the FVB/N background. | ||
| 008450 | FVB-Tg(CAG-luc,-GFP)L2G85Chco/J | Repository- Live |
| Mice homozygous for the CAG-luc-eGFP L2G85 transgene are viable and fertile, with widespread expression of firefly luciferase and enhanced green fluorescence protein directed by the CAG promoter (human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter). Bioluminesence is detected in heart, spleen, muscle, pancreas, skin, thymus and bone marrow. Luciferase activity is not detected in mature erythrocytes, although low levels are detected in erythrocyte precursors and varying levels of activity in all leukocyte subsets tested. Homozygotes have no reported gross physical or behavioral abnormalities. Following luciferin injection, luciferase expression is generally greater in males than females. GFP fluorescence is detected in skin (upper epidermal layers) by fluorescence microscopy. The Donating Investigator reports that for the FVB-Tg(CAG-luc,-GFP)L2G85Chco/J (STOCK#8450) strain, no GFP fluorescence is detected in hematopoiet ..... For more information please see the full phenotype on the strain data sheet | ||
| 013585 | FVB-Tg(Cdh5-tTA)D5Lbjn/J | Repository- Live |
| These transgenic mice express tetracycline regulated transactivator under the direction of the mouse Cdh5, cadherin 5, promoter. When these VE-Cadherin-tTA mice are bred with mice carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE, TetRE or tetO), expression of that gene in blood vessel endothelial cells may be conditionally inactivated with administration of the tetracycline in the double mutant offspring. Tetracycline was used by the Donating Investigator in the original publication Proc Natl Acad Sci 2005;102:128-33. When crossed with a strain carrying a tetracycline-responsive promoter driven lacZ transgene, beta-galactosidase is expression is observed in VEGFR-2, CD31 expressing cells in the blood vessels of bi-transgenic day 13.5 aged embryos. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. | ||
| 006774 | FVB-Tg(Col2a1-cre/ERT)KA3Smac/J | Repository- Live |
| Mice hemizygous or homozygous for the Col2CreERT transgene are viable and fertile. Mice from this founder line (line K from founder mouse A3) have strong tamoxifen-inducible cre expression directed to cells of the chondrogenic lineage (cartilage), with minimal (<0.1%) cre activity in the absence of tamoxifen. The CreERT protein consists of Cre recombinase fused to a mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligand 4-hydroxytamoxifen. Restricted to the cytoplasm, CreERT can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Col2CreERT mice are bred with mice containing a loxP-flanked ..... For more information please see the full phenotype on the strain data sheet | ||
| 006954 | FVB-Tg(Ddx4-cre)1Dcas/J | Repository- Live |
| Mice hemizygous for this Vasa-Cre transgene are viable and fertile. Transgenic cre activity is directed to male and female germ cells starting at embryonic day (e)15-e18. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequence. In such breedings, occasional hemizygous mice may exhibit variegated cre expression in skin epithelium or global cre expression (<20% incidence). Differential parent-of-origin transgene expression is observed. When the mother harbors Vasa-Cre, virtually all progeny undergo global Cre-mediated recombination, even those that do not inherit the transgene (which may be useful in converting a "floxed" allele to a null while obviating the need to perform additional crosses to remove the transgene). To achieve germ-line specific Cre-mediated recombination in offspring, paternal Vasa-Cre mice should be used.
In crosses with some floxed alleles, gl ..... | ||
| 004600 | FVB-Tg(GFAP-cre)25Mes/J | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Mice that are homozygous for the transgene are not viable. This transgenic mouse strain expresses Cre recombinase under the control of the human glial fibrillary acidic protein promoter (GFAP). When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons. Expression activity is also present in periportal cells of the liver. Developmental onset of transgene expression occurs in the dorsal and medial regions of the telencephalon by embryonic day 13.5. In adult cerebellum, only astrocytes are immunoreactive for GFAP or Cre recombinase. This mutant mouse strain represents an effective tool for generating central ner ..... For more information please see the full phenotype on the strain data sheet | ||
| 003718 | FVB-Tg(GadGFP)45704Swn/J | Repository- Live |
| Mice homozygous for the TgN(GadGFP)45704Swn transgene express Enhanced Green Fluorescent Protein (EGFP) under the control of the mouse Gad1 (GAD67) gene promoter. Homozygous mice exhibit no apparent physical or behavioral defects. Transgene expression occurs in a specific subpopulation of hippocampal and cortical GABAergic interneurons that express somatostatin. This subset of interneurons has been shown to be prone to injury during epilepsy, ischemia, and Alzheimer's disease. These transgenic mice are useful for the morphological identification and study of these interneurons in both living and fixed brain tissue. Of note, this strain is one of many fluorescent GABAergic neuron strains, each with unique labeling characteristics (see Stock No. 006334 and Stock No. 006340). | ||
| 005515 | FVB-Tg(ITGAM-DTR/EGFP)34Lan/J | Repository- Live |
| Mice that are homozygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice have a diphtheria toxin (DT) inducible system that transiently depletes macrophages in various tissues. The transgene insert contains a fusion product involving simian diphtheria toxin receptor and green fluorescent protein under the control of the human ITGAM (integrin alpha M) promoter (CD11b). RT-PCR analysis of bone marrow macrophages detects specific transgene expression. Cytological analysis of thioglycollate treated peritoneal cells shows the absence of macrophages. Intraperitoneal injection of DT ablates monocyte/macrophage cells in the peritoneal cavity. Macrophage populations within various tissues demonstrate differential susceptibility DT induced deletion. Following DT administration macrophages are ablated in the peritoneum, kidney and ovary. Macrophage population is restored by day 4 following a single intr ..... For more information please see the full phenotype on the strain data sheet | ||
| 008099 | FVB-Tg(KRT14-rtTA)F42Efu/J | Repository- Live |
| Mice that are hemizygous or homozygous for this transgenic insert are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human keratin 14 (KRT14) gene promoter (active in embryonic and postnatal basal cells of the skin). When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. This strain provides a Tet-On tool that allows the inducible expression of genes in cells expressing KRT14 for hair and skin research. | ||
| 008679 | FVB-Tg(MECP2)1Hzo/J | Repository- Live |
| As hemizygotes, these human methyl CpG binding protein 2 transgenic mice express the gene at ~2-fold wildtype levels in the brain. Enhanced motor and contextual learning, and enhanced hippocampal synaptic plasticity phenotypes begin around 10 weeks of age. After 20 weeks of age, the mice progressively develop neurological impairments, seizures, become hypoactive (characterized by a freezing-like behavior), and ~30% die by one year of age. Forepaw clasping, aggressiveness, and kyphosis are also characteristic in these mice. This strain may be useful in studies of X-linked delayed-onset neurobehavioral disorders, including MECP2 duplication syndrome, mental retardation, and autism. | ||
| 005038 | FVB-Tg(MMTV-Erbb2)NK1Mul/J | Repository- Live |
| These transgenic mice express the activated rat Erbb2 (c-neu) oncogene under the direction of the mouse mammary tumor virus promoter. The activated, or transforming, version of the rat Erbb2 (c-neu) oncogene has a valine to glutamic acid substitution at acid 664, (Val664 to Glu664). Non-uniform and random transgene expression is detected by RNase protection assay in mammary gland epithelium from hemizygous mice. Tumor formation is multifocal, stochastic and matches the expression pattern of the transgene. While no transgene expression is detected at 5 and 8 weeks of age in normal mammary gland tissue, it is detected in adenocarcinomas from older (23 week old) mice. The donating investigator indicates that 50% of transgenic mice derived from the TG.NK founder line, develop tumors within 6-12 months of age. Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. This mutant mouse strain may be useful in studies of bre ..... For more information please see the full phenotype on the strain data sheet | ||
| 016570 | FVB-Tg(Myh6-TRPC3*)6.6Jmol/J | Repository- Live |
| The dnTRPC3 transgene contains amino acids 1-302 of the human transient receptor potential cation channel, subfamily C, member 3 (TRPC3) gene under the control of the mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter. Hemizygotes are viable, fertile and normal in size. TRPC3 is a nonselective monovalent cation channel which is activated by oxidative stress in endothelial cells. These mice express a dominant-negative form of TRPC3. They only express the N-terminus of the gene and lack the transmembrane and cytosolic carboxy terminal domains. TRPC3 transgenic mice exhibit an increase in cardiac calcineurin-nuclear factor of activated T cells (NFAT) transcriptional activity, cardiac hypertrophy, and cardiomyopathy. They show a dosage-dependent increase in cardiac hypertrophy following neuro-endocrine agonist or pressure overload stimulation. | ||
| 016571 | FVB-Tg(Myh6/tetO-Gata6)2Jmol/J | Repository- Live |
| These Gata6 transgenic mice contain the GATA binding protein 6 (Gata6) sequence regulated by a tetracycline operator (tetO), driven by myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6 or α-MHC) promoter/enhancer elements. Hemizygotes are viable, fertile, and normal in size. α-MHC limits overexpression of GATA6 to the heart. GATA6 is a zinc-finger-containing transcription factor expressed in mesoderm and endoderm derived tissues such as heart, liver, lung, gonad, and gut. Along with GATA4, GATA6 is necessary for the development of the embryonic heart and cardiac hypertrophy in the adult heart. When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of GATA6 protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. When bred to mice expressing tTA driven by the α-MHC promoter, double transgenic an ..... For more information please see the full phenotype on the strain data sheet | ||
| 014155 | FVB-Tg(Myh6/tetO-Itpr1)22.3Jmol/J | Repository- Live |
| In this transgenic strain, a conditional Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha )/tetO promoter drives expression of a recombinant mouse "IP3 sponge". The IP3 binding domain encoded by amino acid residues 224-604 of mouse type 1 IP3R (Itpr1 (inositol 1,4,5-triphosphate receptor 1); also called mIP3R1) is fused to glutathione S-transferase (GST). This enables expression of high affinity IP3 chelator fusion protein when the strain is crossed with a driver strain encoding tetracycline transactivator (tTA). | ||
| 014153 | FVB-Tg(Myh6/tetO-Itpr2)3.11Jmol/J | Repository- Live |
| These transgenic animals carry the mouse Itpr2 (inositol 1,4,5-triphosphate receptor 2) gene driven by a conditional Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha)-tetO cardiac-specific promoter.
When crossed with a driver strain encoding the tetracycline transactivator (tTA), a 12-fold increase in protein expression is observed. Overexpression in the heart generates mild baseline cardiac hypertrophy at 3 months of age, but no greater signs of heart disease past 10 months of age. Increased hypertrophic response occurs following pathological/physiological stimuli. | ||
| 014154 | FVB-Tg(Myh6/tetO-Itpr2)4.9Jmol/J | Repository- Live |
| These transgenic animals carry the mouse Itpr2 (inositol 1,4,5-triphosphate receptor 2) driven by a conditional Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha)-tetO cardiac-specific promoter.
When crossed with a driver strain encoding the tetracycline transactivator (tTA), a 5-fold increase in protein expression is observed. No phenotype is observed at baseline, but increased hypertrophic response occurs following certain types of stumuli. Administration of doxycline (Dox) causes near complete extinguishment of ITPR2 expression. | ||
| 013732 | FVB-Tg(NPEPPS)1Skar/J | Repository- Live |
| These mice express a human aminopeptidase puromycin sensitive (hPSA or NPEPPS) gene directed by its endogenous promoter/enhancer regions on a BAC transgene. Mice hemizygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. PSA is a cytosolic aminopeptidase that hydrolyzes N-terminal amino acids. PSA is considered to have neuroprotective qualities due to its ability to degrade certain proteins associated with neurodegenerative diseases (TAU and polyglutamine expansion repeat-containing proteins). hPSA overexpression is seen in the cerebral cortex, spinal cord, cerebellum, hippocampus, and brain stem at a 2-3 fold greater level than endogenous PSA. hPSA activity is also increased in muscle, kidney, and liver. These mice may be useful for studying PSA protection in neurodegenerative disorders. | ||
| 006364 | FVB-Tg(Nr5a1-cre)2Lowl/J | Repository- Live |
| Mice hemizygous for the "Sf1-Cre" transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Transgene expression mimics the mRNA pattern of Nr5a1; with Cre activity observed in steroidogenic factor-1 (SF1)-positive neurons in the ventromedial hypothalamic nucleus (VMH) as well as pituitary, gonad, and adrenal tissue. Expression is also noted in the cerebral cortex and in a few scattered cells in the caudal brainstem of mice derived from line 2 (but not line 7). If bred with mice containing a loxP-flanked sequence of interest, tissue-specific deletion of that genome results in the offspring. Specifically, these cre-expressing mice may be useful in studies involving the hypothalamus, such as body weight homeostasis, obesity, leptin metabolism, or as a reporter strain for SF1-transcription factor activity. | ||
| 006421 | FVB-Tg(Pomc1-hrGFP)1Lowl/J | Repository- Live |
| Hemizygous mice are viable and express humanized Renilla Green Fluorescent Protein (hrGFP, Stratagene) under control of the mouse pro-opiomelanocortin-alpha (Pomc1) promoter. As such, UV light-exposed transgenic brain tissues show GFP fluorescence patterns consistent with the endogenous Pomc1 gene, specifically POMC expressing neurons. The donating investigator reports that hrGFP is more stable and resistant to signal fading compared to other GFP's. The donating investigator reports that transgenic males have smaller seminal vesicles, and breeding transgenic males results in infrequent and very small (<2 pups) litter sizes. When non-transgenic males are crossed with transgenic females, fertility is normal. These POMC-GFP transgenic mice may be useful for studies of neurobiology, energy metabolism, obesity, seizures, and epilepsy. | ||
| 005688 | FVB-Tg(Rag2-EGFP)1Mnz/J | Repository- Live |
| Mice hemizygous for the NG-BAC transgene are viable and fertile, with expression of enhanced green fluorescent protein (EGFP) directed by the Rag2 promoter. EGFP expression is high in pro-B cells and decreases during B cell maturation. Similarly, EGFP induction in T cells occurs in the late CD4/CD8 double negative stage and expression decreases during maturation. While mature cells do not express EGFP, lingering protein can be detected. The transgene does not hinder B cell function or T cell proliferation. These NG-BAC transgenic mice can be used in studies of B and T cell development as EGFP fluorescence reflects Rag2 protein expression. | ||
| 005110 | FVB-Tg(Sod1*G86R)M1Jwg/J | Repository- Live |
| These transgenic mice express the missense mutant mouse Sod1 under the control of the endogenous promoter. The missense mutation is a point mutation in exon 4 resulting in a glycine-86 to arginine substitution,which corresponds to amino acid position 86 in the human SOD1 protein. Transgene expression is detected by RT-PCR analysis of brain, spinal cord, and other tissues. Onset of progressive loss of motor function begins at 3-4 months of age with hind limb spastic paralysis and muscle wasting. Transgenic mice do not survive beyond 4 months of age. Histological analysis of spinal cord ventral horns, brain stem and neocortex reveals neurodegeneration and abnormal neurites. Affected mice do not exhibit diminished SOD1 activity. This mutant mouse strain may be useful in studies of amyotrophic lateral sclerosis. | ||
| 004938 | FVB-Tg(YAC128)53Hay/J | Repository- Live |
| These transgenic mice express the human huntingtin protein containing a 128 CAG repeat expansion. Human huntingtin mRNA and protein is detected. Hyperkinesis begins at 3 months of age with progressive motor impairment appearing at 6 months of age. This is followed by progressive neurodegeneration, starting at 9 months of age, and hypokinesis at 12 months. The motor dysfunction, Rotorod deficit, is correlated with neuronal loss. Mutants exhibit decreased brain weight and reduced striatal and cortical volumes. 18% shrinkage of striatal neurons is observed in 12 month old mutants. A significant decrease (15%) in the number of striatal neurons occurs by 12 months of age. Nuclear huntingtin aggregate inclusions of striatal neurons from 18 month old mutant mice are detected at the light microscopy level. This mutant mouse strain represents a model that may be useful in studies of Huntington's disease. | ||
| 013778 | FVB-Tg(tetO-Cacnb2)1Jmol/J | Repository- Live |
| Expression of Cacnb2 (calcium channel, voltage-dependent, beta 2 subunit) is regulated by a tetracycline operator (tetO); also called tetracycline-responsive element (TRE, TetRE) or tet-operator) in this transgenic strain. When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of CACNB2 protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. When crossed with a Myh6-tTA strain (e.g. Stock No. 003170), double transgenic mice show increased Ca2+ influx through cardiac L-type Ca2+ channels resulting in Ca2+ overload, myocyte disorganization, interstitial fibrosis, and cell death. Compound mutant animals show a 7.4-fold increase in CACNB2 expression. This strain may be useful in studies of cardiomyopathy. | ||
| 013780 | FVB-Tg(tetO-Cib1)1Jmol/J | Repository- Live |
| Expression of Cib1 (calcium and integrin binding 1 (calmyrin)) is regulated by a tetracycline operator (tetO); also called tetracycline-responsive element (TRE, TetRE) or tet-operator) in this transgenic strain. When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of CIB1 protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. When crossed with a Myh6-tTA strain (e.g. Stock No. 003170), double transgenic mice show enhanced cardiac hypertrophy in response to pressure overload or calcineurin signalling. This strain may be useful in studies of cardiac hypertrophy. | ||
| 012387 | FVB-Tg(tetO-Ppargc1a)1Dpk/J | Repository- Live |
| These transgenic mice express Ppargc1a (peroxisome proliferative activated receptor, gamma, coactivator 1 alpha) and a myc-his tag regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator). When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue Ppargc1a expression may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring. This strain may be useful for studies of metabolism, cardiomyopathy, and mitochodrial function. | ||
| 008782 | FVB.Cg-Smn1tm1Msd Tg(SMN2)89Ahmb Tg(SMN2*A111G)588Ahmb/J | Repository- Live |
| Mice that are homozygous for the Tg(SMN2*A111G)588Ahmb transgene and homozygous for the Smn1tm1Msd targeted mutation are not viable. Mice that are homozygous for the Tg(SMN2*A111G)588Ahmb transgene, homozygous for the Smn1tm1Msd targeted mutation and hemizygous or homozygous for the Tg(SMN2)89Ahmb transgene are viable and survive for longer than one year. Expression of the Tg(SMN2*A111G)588Ahmb in founder line 588 is detected in the spinal cord, forebrain and liver by RT-PCR. This mutant mouse strain may be useful in studies of spinal muscular atrophy. Importation of this model was supported by the Spinal Muscular Atrophy Foundation. | ||
| 009134 | FVB.Cg-Smn1tm1Msd Tg(SMN2)89Ahmb Tg(SMN2*A111G)591Ahmb/J | Repository- Live |
| Mice that are homozygous for the Tg(SMN2*A111G)591Ahmb transgene and homozygous for the Smn1tm1Msd targeted mutation are not viable. Mice that are hemizygous for the Tg(SMN2*A111G)591Ahmb transgene, homozygous for the Smn1tm1Msd targeted mutation and hemizygous or homozygous for the Tg(SMN2)89Ahmb transgene are viable and survive for longer than one year. The Jackson Laboratory has been unable to generate animals homozygous for all three alleles. Strong expression of the Tg(SMN2*A111G)591Ahmb transgene in founder line 591 is detected in the spinal cord, and weaker expression is detected in forebrain and liver by RT-PCR. This mutant mouse strain may be useful in studies of spinal muscular atrophy. Importation of this model was supported by the Spinal Muscular Atrophy Foundation. | ||
| 003516 | FVB.Cg-Tg(CAG-EGFP)B5Nagy/J | Repository- Live |
| This transgenic strain expresses an Enhanced Green Fluorescent Protein (Clontech) driven by chicken beta-actin promoter and CMV intermediate early enhancer. Mice, and cells derived from them, are distinguished from wildtype on the basis of fluorescence. The transgene is expressed in all nucleated embryonic tissues. Cells and tissues with increased hemoglobin content exhibit reduced fluorescence as development progresses. In newborn and adult mice, the entire organ system expresses EGFP. Though widespread, expression levels vary between different organs. This strain can be used as a source of fluorescently marked cells or tissues. Animals from stock 003516 homozygous for Tg(CAG-EGFP)B5Nagy allele have displayed an increased incidence of lymphoma compared to animals hemizygous for this allele. Lymphoma in homozygous breeders for stock 003516 has been observed as early as 4 months of age. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles ..... | ||
| 004363 | FVB.Cg-Tg(MMTV-vHaras)SH1Led/J | Repository- Live |
| These transgenic mice express the Harvey RAS (human) gene under the direction of the mouse mammary tumor virus promoter. The majority of expression of the transgene is detected in the mammary tissue and salivary glands. Both male and female transgenic mice develop malignant lymphoid tissue and mammary and salivary gland tumors as early as 5 weeks of age. Half of the female transgenic animals develop tumors by 6 months of age. Histological analysis of mammary tumors revealed most of the tumors were adenocarcinomas and were locally invasive with some cases of metastasis to liver and/or lung. The tumors were found to express the transgene transcript. Protein-tyrosine phosphatase epsilon mRNA and protein was found to be highly expressed only in mammary tumors. In 20% of the transgenic mice diffuse benign hyperplasia develops in the Harderian lacrimal gland causing bilateral exophthalmia. The donating investigator reports hemizygous females develop tumors by delivery of their first litter ( ..... For more information please see the full phenotype on the strain data sheet | ||
| 005058 | FVB.Cg-Tg(SMN2)2Hung Smn1tm1Hung/J | Repository- Live |
| Mice that are homozygous for the Smn1 targeted mutation and hemizygous for the SMN2 transgene are viable, fertile and exhibit short and thickened tails. RT-PCR analysis detects alternative splicing of the transgene. Histological examination of tail tissue reveals atrophic muscles and subcutaneous edema. Skeletal muscle tissue has fewer myocytes and atrophic muscle bundles. Large motor neurons in the anterior horns of the spinal cord degenerate and are lost. There is a strong correlation between estimated copy number of the transgene and severity of the phenotype. These mice exhibit a molecular and progressive neurodegenerative phenotype similar to Type III spinal muscular atrophy. Mice that are homozygous for the targeted mutation and do not carry the transgene have an embryonic lethal phenotype, failing to survive past embryonic day 6.5. Importation of this model was supported by the Spinal Muscular Atrophy Foundation. | ||
| 005024 | FVB.Cg-Tg(SMN2)89Ahmb Smn1tm1Msd/J | Repository- Live |
| Mice that are homozygous for the targeted mutant Smn1 allele and carry the SMN2 transgene exhibit symptoms and neuropathology similar to patients afflicted with type I proximal spinal muscular atrophy (SMA). In the initial characterization by the donating investigator, mice were either stillborn or survived 4-6 days. Mice that died at or shortly after birth were slightly smaller (1.33 g. vs. 1.51 g.) than normal littermates. Mice that survive for several days are indistinguishable from normal littermates in the first 48 hours, after which they exhibit decreased suckling and movement, labored breathing and tremoring limbs. Mice succumbing at this later time point are noticeably smaller than normal littermates (1.47 g vs. 4.59). A bell-shaped trunk is also noticeable in affected mice, presumably from intercostal muscle weakness, a characteristic of type I SMA. Histological analysis indicates that affected mice that survive to day 5 exhibit a loss of motor neurons from spina ..... For more information please see the full phenotype on the strain data sheet | ||
| 005026 | FVB.Cg-Tg(SMN2)89Ahmb Tg(SMN1*A2G)2023Ahmb Smn1tm1Msd/J | Repository- Live |
| Mice that are homozygous for the targeted mutant Smn allele and homozygous for the SMN2transgene and hemizygous for the SMN1*A2G transgenes exhibit symptoms and neuropathology similar to patients afflicted with type III (mild) proximal spinal muscular atrophy (SMA). These same animals with only one copy of the SMN2transgene are 20%-40% smaller than unaffected mice. At 3 weeks of age they become less active and show signs of muscle weakness. The mice have a shortened lifespan (less than a year) near the end of which they exhibit reduced movement, diminished grooming, shallow breathing and considerable weight loss. Immunohistochemical analysis of spinal cord tissue from 1 month-old animals indicates the presence of cytoplasmic SMN protein and intranuclear aggregates (gems) of the SMN protein. The number of gems is however, fewer than the number found in age matched control tissues. Histological analysis of muscle tissue (gastrocnemius, quadriceps, and intercostals mu ..... For more information please see the full phenotype on the strain data sheet | ||
| 005025 | FVB.Cg-Tg(SMN2*delta7)4299Ahmb Tg(SMN2)89Ahmb Smn1tm1Msd/J | Repository- Live |
| This triple mutant mouse harbors two transgenic alleles and a single targeted mutant. The Tg(SMN2*delta7)4299Ahmb allele consists of a SMA cDNA lacking exon 7 whereas the Tg(SMN2)89Ahmb allele consists of the entire human SMN2 gene. Mice that are homozygous for the targeted mutant Smn allele and homozygous for the two transgenic alleles exhibit symptoms and neuropathology similar to patients afflicted with proximal spinal muscular atrophy (SMA). At birth, triple mutants are noticeably smaller than normal littermates. By day 5, signs of muscle weakness are apparent and become progressively more pronounced over the following week as the mice display an abnormal gait, shakiness in the hind limbs and a tendency to fall over. Mean survival is approximately 13 days. Immunocytochemical analysis indicates that dystrophin expression is normal, however fibers isolated from the gastrocnemius muscle of a 14 day old triple mutant clearly show evidence of atrophy. Importation ..... | ||
| 003314 | FVB/N-Tg(EIIa-cre)C5379Lmgd/J | Repository- Live |
| This line carries a Cre transgene under the control of the adenovirus EIIa promoter that targets expression of Cre recombinase to the early mouse embryo. Cre expression is thought to occur prior to implantation in the uterine wall. A mosaic pattern of expression is commonly observed. Cre-mediated recombination occurs in a wide range of tissues, including the germ cells that transmit the genetic alteration to progeny.
View cre expression characterization. | ||
| 012630 | FVB/N-Tg(GFAP-HTT*160Q)31Xjl/J | Repository- Live |
| HTT-160Q-31 transgenic mice have the human glial fibrillary acidic protein (GFAP) promoter directing expression of the human huntingtin (HTT) gene in astrocytes, a glial cell that removes extracellular glutamate in the brain. Hemizygous GFAP-HD mice are viable and fertile. HTT-160Q-31 mice exhibit weight loss, deficient motor function, and die earlier than control mice. HTT-160Q-31 binds with Sp1 transcription factor decreasing the expression of glutamate transporter in astrocytes by reducing the association of Sp1 with the glutamate transporter promoter. This leads to a decrease in extracellular glutamate uptake by glial cells and may cause the glutamate excitotoxicity associated with Huntington's disease. These mice may be useful for studying the pathology and neurodegeneration associated with glutamate excitotoxicity in Huntington's disease. | ||
| 003257 | FVB/N-Tg(GFAPGFP)14Mes/J | Repository- Live |
| Transgenic mice overexpress Green Fluorescent Protein under the control of the astrocyte-specific glial fibrillary acidic protein promoter. Bright fluorescence is observed in the cell bodies and processes of unfixed or fixed astrocyte preparations throughout the CNS of hemizygous mice. In addition retinal Mullers cells expressed the GFP transgene in response to degeneration of neighboring photoreceptors. These mice provide a method to follow changes in astrocyte morphology during development or disease processes. | ||
| 008197 | FVB/N-Tg(HTT*97Q)IXwy/J | Repository- Live |
| Mice hemizygous for the BACHD transgene are viable and fertile. Under the control of endogenous human htt regulatory machinery, BACHD mice have relatively high expression levels of a neuropathogenic, full-length human mutant Huntingtin (fl-mhtt) modified to harbor a loxP-flanked human mutant htt exon 1 sequence (containing 97 mixed CAA-CAG repeats encoding a continuous polyglutamine (polyQ) stretch). Prior to Cre recombinase exposure, BACHD mice exhibit progressive motor deficits, neuronal synaptic dysfunction, and selective late-onset neuropathology without somatic polyQ repeat instability in the aged brain. Moreover, BACHD mice reproduce a mhtt aggregation pattern reminiscent of that in adult-onset Huntington's disease (HD). Importantly, a relatively steady-state level of predominantly fl-mhtt and a small amount of mhtt N-terminal fragments present in both the nucleus and cytoplasm, are responsible for the onset and progression of neuropathology. Upon exposure to ..... For more information please see the full phenotype on the strain data sheet | ||
| 008232 | FVB/N-Tg(Ins2-IAPP)RHFSoel/J | Repository- Live |
| Mice homozygous for the RIPHAT transgene are viable and fertile, with expression of human islet amyloid polypeptide (h-IAPP) under the regulatory control of the rat insulin II promoter. While h-IAPP RNA from the transgene is observed in pancreas, kidney, and stomach, h-IAPP protein is reported only in pancreas tissues. Hemizygous mice show no symptoms of spontaneous disease. Homozygous males spontaneously develop diabetes mellitus due to beta-cell death, associated with impaired insulin secretion (hypoinsulinemia), hyperglycemia, and abnormal intracellular aggregates of h-IAPP (the donating investigator reports that extracellular aggregates are not found on this strain background). Homozygous male onset is between 4-8 weeks of age with death around 16 weeks of age. Homozygous females exhibit a less severe phenotype. These RIPHAT transgenic mice may be useful in studying the beta-cell destruction and islet amyloid deposition associated with non-insulin-dependent diabetes mellitus (NIDDM ..... For more information please see the full phenotype on the strain data sheet | ||
| 009610 | FVB/N-Tg(LRRK2)1Cjli/J | Repository- Live |
| Mice hemizygous for the BAC LRRK2 transgene are viable and fertile, with expression of a wild-type human leucine-rich repeat kinase 2 (LRRK2) gene directed by its endogenous promoter/enhancer regions on the BAC transgene. These BAC LRRK2 mice (also called WT-OX mice) "overexpress" the wildtype human LRRK2 protein in cortex, cerebellum, striatum and ventral midbrain at an approximately five- to ten-fold greater level than endogenous mouse Lrrk2, and are a control strain for the BAC LRRK2R1441G Parkinson's disease strain (Stock No. 009604). Contrary to the hypokinetic motor deficit of the BAC LRRK2R1441G Parkinson's disease mice, WT-OX mice exhibit slightly increased motor activities compared to nontransgenic wild-type controls. | ||
| 009609 | FVB/N-Tg(LRRK2*G2019S)1Cjli/J | Repository- Live |
| Mice hemizygous for the BAC LRRK2 (G2019S) transgene are viable and fertile, with expression of a mutant form of human leucine-rich repeat kinase 2 (LRRK2*G2019S) associated with autosomal dominant, late-onset Parkinson's disease directed by the endogenous LRRK2 promoter/enhancer regions on the BAC transgene. The phenotype of these LRRK2*G2019S mice is not yet characterized. These mice represent an in vivo model for studying the Parkinson's disease pathogenesis and neurodegeneration elicited by the dominant toxic effects of mutant LRRK2*G2019S expression. | ||
| 009604 | FVB/N-Tg(LRRK2*R1441G)135Cjli/J | Repository- Live |
| Mice hemizygous for the BAC LRRK2R1441G transgene are viable and fertile, with expression of a mutant form of human leucine-rich repeat kinase 2 (LRRK2*R1441G) associated with autosomal dominant, late-onset Parkinson's disease directed by the endogenous LRRK2 promoter/enhancer regions on the BAC transgene. LRRK2R1441G mice from founder line RP135 express the mutant protein in cortex, cerebellum, striatum and ventral midbrain at an approximately five- to ten-fold greater level than endogenous mouse Lrrk2. LRRK2R1441G mice exhibit multiple late-onset and progressive characteristics of Parkinson's disease; including hypokinetic motor deficits (reversible with administration of levodopa or apomorphine [a direct-acting dopamine agonist]), progressive dopaminergic neuron dysfunction and degeneration, axon injury pathology, and hyperphosphorylated tau. These LRRK2R1441G mice recapitulate the motor behavioral, neurochemical, and histopa ..... For more information please see the full phenotype on the strain data sheet | ||
| 002374 | FVB/N-Tg(MMTV-PyVT)634Mul/J | Repository- Live |
| Mice carrying the (MMTV-PyVT) transgene are viable, but show loss of lactational ability coincident with transgene expression. Female carriers develop palpable mammary tumors as early as 5 weeks of age. Adenocarcinomas arise in virgin and breeder females as well as males, which are multifocal, highly fibrotic, and involve the entire mammary fat pad. Males also develop adenocarcinoma of the seminal vesicles and hemangiomas. Pulmonary metastases are observed in 80-94% of tumor-bearing female mice. Transgene expression is detected at high levels in male and female mammary glands. Lower levels are detected in salivary gland, seminal vesicles, ovaries, and lungs (believed to be the result of pulmonary metastases). | ||
| 012460 | FVB/N-Tg(Myh6-Gnaq)40Gwd/J | Repository- Live |
| Mice hemizygous for the Myh6-Gnaq allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The donating investigator states that hemizygous females develop peripartum cardiomyopathy. Expression of Gαq is regulated by an α-myosin heavy chain (Myh6) promoter, which leads to overexpression of Gαq in the heart. This overexpression results in cardiac hypertrophy, defined as a conserved program of fetal gene expression, increased heart weight, and increased cardiomyocyte size, which severely compromises systolic cardiac function, and results in overt cardiac failure. Upon experimental pressure overloading, the mice progress rapidly to heart failure. These mice may be useful for studying the biochemical and physiologic phenotype resembling both the compensated and decompensated phases of human cardiac hypertrophy. | ||
| 002856 | FVB/N-Tg(TIE2-lacZ)182Sato/J | Repository- Live |
| Transgenic mice carry a beta-galactosidase reporter gene under the control of the murine Tek (Tie2) promoter. LacZ is expressed specifically in vascular endothelial cells in embryonic and adult mice. The transgenic line may be useful when crossed with tumor producing strains and the transgene used to visualize neovascularization during tumorigenesis. | ||
| 006143 | FVB/N-Tg(Thy1-cre)1Vln/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is observed in nearly all neurons in cortex and hippocampus. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in postnatal, neuron-specific deletion of the flanked genome. These mice may be useful in studies of the nervous system, including Alzheimer's disease.
View cre expression characterization. | ||
| 012370 | FVB/NJ-Tg(Hspa1a-luc,-EGFP)2Chco/J | Repository- Live |
| Hemizygous FVB.Hsp70-luc-2A-eGFP (or Hsp70A1-L2G) transgenic mice are viable and fertile, with expression of firefly luciferase and enhanced green fluorescent protein directed by the mouse Hsp70A1 promoter (from the mouse Hspa1a locus). Following cellular stress, both eGFP fluorescence and luciferase expression may be detected in those cells (note that the luciferin substrate is needed for in vivo bioluminescence imaging (BLI)). As heat shock protein (Hsp) expression correlates with a cytoprotective effect in cultured cells and with improved healing of damaged tissues in vivo, these Hsp70A1-L2G transgenic mice may be useful as a rapid luciferase/fluorescent indicator of cellular stress (such as thermal, burn, or laser tissue injury), cell survival, and wound healing.
Of note, the donating investigator reports choosing Hsp70A1-L2G mice with high eGFP fluorescence or high luciferase activity to use for breeding (and therefore the colony may have multiple in ..... | ||
| 009090 | FVB/NJ-Tg(Slc6a3-PARK2*Q311X)AXwy/J | Repository- Live |
| Hemizygous Parkin-Q311X(A) mice are viable and fertile, with expression of a FLAG-tagged, C-terminal truncated human parkin-Q311X mutation associated with Turkish early-onset Parkinson's disease directed to dopaminergic neurons of the substantia nigra pars compacta (SNc) and ventral tegmentum area (VTA) by the mouse Slc6a3 promoter/enhancer sequences. Parkin-Q311X(A) mice (derived from founder line A) have expression of the FLAG-tagged parkin-Q311X protein in dopaminergic neurons at a level that is approximately equivalent to or just below that expected from a heterozygous endogenous parkin allele. Parkin-Q311X(A) mice exhibit multiple late-onset and progressive hypokinetic motor deficits, progressive dopaminergic neuron dysfunction and degeneration, and age-dependent accumulation of proteinase K-resistant endogenous alpha-synuclein. Compared to founder line D, Parkin-Q311X(A) mice have a higher transgene copy number that results in more robust and earlier onset of hypokinetic m ..... For more information please see the full phenotype on the strain data sheet | ||
| 010710 | FVB;129S6-Sncatm1Nbm Tg(SNCA)1Nbm/J | Repository- Live |
| These PAC-Tg(SNCAWT);Snca-/- mice are viable and fertile, harboring a Snca knockout allele and a transgene encoding the human α-synuclein. As homozygotes, expression of endogenous mouse α-synuclein is abolished and replaced by human α-synuclein from the two total insertions of the PAC-Tg(SNCAWT). While brain RNA expression of SNCAWT is more than 50-fold greater compared to normal endogenous mouse α-synuclein, protein levels are only 1 to 1.5-fold greater. In colon however, both RNA and protein expression of SNCAWT are ~100-fold greater than normal endogenous mouse α-synuclein. PAC-Tg(SNCAWT);Snca-/- mice do not show any enteric nervous system abnormalities or widespread α-synuclein aggregation in brain or colon. No detectable motor behavior impairments, autonomic abnormalities, olfactory dysfunction, dopaminergic deficits, Lewy body inclusions or neurodegeneration are ..... For more information please see the full phenotype on the strain data sheet | ||
| 010788 | FVB;129S6-Sncatm1Nbm Tg(SNCA*A30P)1Nbm Tg(SNCA*A30P)2Nbm/J | Repository- Live |
| These double transgenic homozygous mice (dbl-PAC-Tg(SNCAA30P);Snca-/- mice) are viable and fertile, harboring a Snca knockout allele and two independently inserted transgenes encoding the human A30P-mutant α-synuclein associated with autosomal dominant Parkinson's disease. As homozygotes, expression of endogenous mouse α-synuclein is abolished and replaced by α-synuclein*A30P from the PAC-Tg(SNCAA30P). Because PAC-Tg(SNCAA30P) line 1 inserted on the X chromosome and line 2 inserted on a somatic chromosome, homozygous females have four total insertions and homozygous males have three total insertions. While brain RNA expression of SNCAA30P is more than 10-fold greater compared to normal endogenous mouse α-synuclein, protein levels are only 1 to 1.5-fold greater. In colon however, both RNA and protein expression of SNCAA30P are ~80-fold greater than normal endogenous mouse α-synuclein. ..... For more information please see the full phenotype on the strain data sheet | ||
| 010799 | FVB;129S6-Sncatm1Nbm Tg(SNCA*A53T)1Nbm Tg(SNCA*A53T)2Nbm/J | Repository- Live |
| These double transgenic homozygous mice (dbl-PAC-Tg(SNCAA53T);Snca-/- mice) are viable and fertile, harboring a Snca knockout allele and two independently inserted transgenes encoding the human A53T-mutant α-synuclein associated with autosomal dominant Parkinson's disease. As homozygotes, expression of endogenous mouse α-synuclein is abolished and replaced by α-synuclein*A53T from the four total insertions of the PAC-Tg(SNCAA53T). While brain RNA expression of SNCAA53T is ~10-fold greater compared to normal endogenous mouse α-synuclein, protein levels are only 1 to 1.5-fold greater. In colon however, both RNA and protein expression of SNCAA53T are ~80-fold greater than normal endogenous mouse α-synuclein. By three months of age, dbl-PAC-Tg(SNCAA53T);Snca-/- mice show robust abnormalities in enteric nervous system function (impaired colonic motility [males only] and prolonge ..... For more information please see the full phenotype on the strain data sheet | ||
| 013233 | NOD.B6-Tg(Itgax-cre,-EGFP)4097Ach/J | Repository- Live |
| Mice hemizygous for this Itgax-cre,-EGFP (Itgax, commonly referred to as CD11c) transgene are viable and fertile. The CD11c promoter directs bicistronic Cre and EGFP protein expression to dendritic cells (DCs). Expression of EGFP is expected to have equimolar expression with Cre recombinase. When bred with any mouse containing a loxP-flanked sequence of interest, the resulting offspring can have Cre-mediated recombination of the flanked sequence. These CD11c-Cre-GFP transgenic mice may be useful for immunological studies, specifically in Type 1 Diabetes, utilizing Cre-lox technology or fluorescent protein expression in dendritic cells, . | ||
| 012478 | NOD.Cg-Prkdcscid Tg(HLA-DRA*0101,HLA-DRB1*0101)1Dmz/GckJ | Repository- Live |
| Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. NOD.CB17-Prkdcscid mice with the DR1 transgene survive to 14 months as compared to 8.5 months without the transgene. Physiological parameters in this strain are similar to NOD.CB17-Prkdcscid mice. Engraftment of human hematopoietic cells occurs regardless of DR1 genotype and is enhanced by intrahepatic engraftment using neonatal mice. Following injection, 15-20% human CD45+ cells are detected in peripheral blood. Short-term engrafted cord blood mononuclear cells can mount an immune response to tumor-associated antigen. This mutant mouse strain may be useful for xenotransplantation and vaccine development. | ||
| 009617 | NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(HLA-A2.1)1Enge/SzJ | Repository- Live |
| The NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(HLA-A2.1)1Enge/SzJ mice, commonly known as NOD scid gamma, HLA-A2.1 (NSG-A2), express human class I MHC Ag HLA-A2.1 , but do not express the Prkdc gene nor the X-linked Il2rg gene. Triple mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. NSG mice, carrying the HLA-A2.1 transgene (homozygous or hemizygous), overcome one of the limitations of immune response seen in the NSG model (Stock No. 005557), in that they develop protective T cell responses against human viral infections, specifically Epstein-Barr virus, exhibiting traits similar to those seen in humans. This model may be useful for studying response to human antigens and represent a unique preclinical model for vaccine development.
Please note that this strain carries the true null interleukin-2 receptor gamma chain mutation and ..... | ||
| 014570 | NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(HLA-A/H2-D/B2M)1Dvs/SzJ | Repository- Live |
| Mice that are homozygous for the Prkdcscid and Il2rgtm1Wjl alleles (males are hemizygous for the Il2rgtm1Wjl allele) Tg(HLA-A2/H2-D/B2m)1Dvs/SzJ are immunodeficient and express human HLA class 1 heavy and light chains. HLA-A2 and B2M proteins are detected on the surface of NSG-HLA-A2/HHD splenocytes by flow cytometry analysis. Transplantation of purified human hematopoietic stem cells into newborn NSG-HLA-A2/HHD mice establishes a humanized immune microenvironment allowing functional maturation of human hematopoietic cells. Epstein-Barr virus (EBV) infection results in EBV-associated B cell proliferation and HLA-restricted EBV antigen-specific effector memory CTLs. | ||
| 012479 | NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(HLA-DRA*0101,HLA-DRB1*0101)1Dmz/GckRolyJ | Repository- Live |
| Mice that are homozygous for the targeted transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mutant mice combine the features of the NOD/ShiLtJ background, the severe combined immune deficiency mutation (scid), IL2 receptor gamma chain deficiency and a human/mouse chimeric MHC Class II transgene (Tg(HLA-DRB1*01)1Dmz). This mutant mouse strain is expected to be useful for xenotransplantation and vaccine development. | ||
| 013062 | NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ | Repository- Live |
| These mice contain three coinjected transgenes, human interleukin-3 (IL-3), human granulocyte/macrophage-stimulating factor (GM-CSF), and human Steel factor (SF) gene, each driven by a human cytomegalovirus promoter/enhancer sequence. These mice are maintained on the NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice (Stock No. 005557) background. These mice constitutively produce 2-4 ng/ml serum levels of human IL-3, GM-CSF, and SF. The Il2rg-/- specific NOD.SCID background supports human and murine hematopoietic cell engraftment, and suppresses human erythropoiesis, enhances human myelopoiesis, and reduces human B-lymphopoiesis in mice after transplant of human bone marrow or fetal liver cells. | ||
| 011066 | NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CSF2)2Ygy Tg(IL3)1Ygy Tg(KITLG)3Ygy/YgyJGckRolyJ | Repository- Live |
| These mutant mice carry the Prkdcscid and Il2rgtm1Wjl alleles and the Tg(IL3)1Ygy, Tg(CSF2)2Ygy and Tg(KITLG)3Ygy transgenes. The Tg(CSF2)2Ygy transgene contains the porcine colony stimulating factor 2 (granulocyte-macrophage) sequence (CSF2) under the control of the human cytomegalovirus promoter. The Tg(IL3)1Ygy transgene contains the porcine interleukin 3 sequence under the control of the human cytomegalovirus promoter. The Tg(KITLG)3Ygy transgene contains the porcine kit ligand (KITL) under the control of the human cytomegalovirus promoter. The 3 transgenes were coinjected together in the original transgenic strain used to generate this mutant strain. | ||
| 009377 | NOD.Cg-Rag1tm1Mom Tg(TcraBDC12-4.1)10Jos Tg(TcrbBDC12-4.1)82Gse/J | Repository- Live |
| This double transgenic, targeted mutation strain carries a rearranged Tcr beta gene and a Tcr alpha gene from the pathogenic anti-insulin CD4+ T-cell clone BDC12-4.1 and NOD.Rag1tm1Mom targeted mutation (Stock No. 003729). Triple mutant mice are viable and fertile. When the congenic NOD double transgenic is combined with a homozygous Rag1tm1Mom mutation, recombination of the endogenous TCR and immunoglobulin genes is prevented. As a result, mature T cells in these mice express only the BDC12-4.1 TCR. Further, approximately 50% of the TCR double transgenic, Rag1tm1Mom homozygote mice develop diabetes by 30 weeks of age. Histopathology indicates severe insulitis and/or destruction of all insulin-producing cells (Jasinski et al 2006). This BDC12-4.1 TCR double transgenic, Rag1 deficient strain is useful for further studying the pathogenesis of insulin autoimmuni ..... For more information please see the full phenotype on the strain data sheet | ||
| 008173 | NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ | Repository- Live |
| Mice hemizygous for this "MIP-GFP" transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Green Fluorescent Protein under the control of mouse insulin 1 promoter. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adult. The human growth hormone sequence in the transgenic insert enhances expression of the EGFP but is not expressed. The donating investigator reports NOD transgenic female mice develop insulitis and autoimmune diabetes at a similar rate and kinetics as female wildtype controls, 62% and 58% respectively, while surprisingly, transgenic males develop insulitis and diabetes at a higher rate than male wildtype controls, 65% and 10% respectively. This transgenic MIP-GFP mouse strain is useful in studies of diabetes and pancreatic beta islet cell biology.
In ..... | ||
| 004460 | NOD.Cg-Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/DoiJ | Repository- Live |
| These transgenic mice carry both rearranged TCR alpha and beta genes from the cytotoxic CD4+ T cell clone BDC-2.5. When paired with a homozygous Rag1tm1Mom mutation (such as in Stock No. 003729), recombination of endogenous TCR and Ig is prevented so that mature T cells in these mice express only the BDC2.5 TCR. On the NOD background, mice carrying the transgenes have a reduced incidence of diabetes relative to NOD/ShiLtJ controls (12% incidence at age 30 weeks). When coupled with the homozygous Rag1tm1Mom mutation, mice develop diabetes extremely early (mean age of 25 days). (Katz et al 1993, Gonzalez et al 2001, Mombaerts et al 1992) | ||
| 010526 | NOD.Cg-Tg(TcraTcrbNY4.1)1Pesa/DvsJ | Repository- Live |
| NOD.Cg-Tg(TcraTcrbNY4.1Pesa/DvsJ, commonly called NY4.1-NOD, express rearranged Tcrα and Tcrβ transgenes derived from the pancreatic beta cell-cytotoxic CD4+ T cell clone NY4.1. CD4+CD8- thymocyte derived T cells are skewed toward V β11+ expression when compared to wild type controls. Transgenic females and males exhibit dramatically accelerated diabetes, the cumulative transgenic female diabetes incidence is similar to wildtype females; while transgenic males exhibit increased incidence compared to wildtype controls. Overall kinetics of disease are remarkably similar to their wild type cohorts. Insulitis scores of 3 week old transgenic mice are significantly lower, while insulitis scores of 6 week olds are significantly more severe than in wild types controls, Schmidt et al, 1997, J. Exp Med 186, 1059-1075. Transgenic animals bearing NY4.1 TCR alpha and beta transgenes offer a source of CTL ..... For more information please see the full phenotype on the strain data sheet | ||
| 005868 | NOD.Cg-Tg(TcraTcrbNY8.3)1Pesa/DvsJ | Repository- Live |
| NOD.Cg-Tg(TcraTcrbNY8.3)1Pesa/DvsJ, commonly called 8.3-NOD, express rearranged Tcra and Tcrb transgenes derived from the pancreatic beta cell-cytotoxic CD8+ T cell clone NY8.3. CD4-CD8+ thymocytes and lymph derived T cells are skewed toward VB8.1/2+ expression when compared to wild type controls. Although, transgenic mice exhibit dramatically accelerated diabetes, the cumulative diabetes incidence and kinetics of disease are remarkably similar to their wild type cohorts. Insulitis scores of 3 week old transgene+ mice was significantly lower, while insulitis scores of 6 week olds were significantly more severe than in wild types controls, Verdaguer J et al, 1997, J. Exp Med 186, 1663-1676.
Transgenic animals bearing both TCR transgenes offer a source of CTL precursors useful in examining the diversity of beta cell peptides recognized by the autoreactive CD8+ T lymphocytes contributing to th ..... For more information please see the full phenotype on the strain data sheet | ||
| 010542 | NOD.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ | Repository- Live |
| These L2G85.NOD mice harbor the CAG-luc-eGFP L2G85 transgene. Mice homozygous for the CAG-luc-eGFP L2G85 transgene are viable and fertile, with widespread expression of firefly luciferase and enhanced green fluorescence protein directed by the CAG promoter (human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter). Bioluminesence is detected in heart, spleen, muscle, pancreas, skin, thymus and bone marrow. Luciferase activity is not detected in mature erythrocytes, although low levels are detected in erythrocyte precursors and varying levels of activity in all leukocyte subsets tested. Homozygotes have no reported gross physical or behavioral abnormalities. Following luciferin injection, luciferase expression is generally greater in males than females. GFP fluorescence is detected in skin (upper epidermal layers) by fluorescence microscopy. The Donating Investigator reports that for the FVB-Tg(CAG-luc,-GFP)L2G85Chco/J (STOC ..... For more information please see the full phenotype on the strain data sheet | ||
| 008547 | NOD.FVB-Tg(ITGAM-DTR/EGFP)34Lan/JdkJ | Repository- Live |
| Transgenic mice have a diphtheria toxin (DT) inducible system that transiently depletes macrophages. The transgene insert contains a fusion product involving simian diphtheria toxin receptor and green fluorescent protein under the control of the human ITGAM (integrin alpha M) promoter (CD11b). RT-PCR analysis of bone marrow macrophages detects specific transgene expression. Cytological analysis of thioglycollate treated peritoneal cells shows the absence of macrophages. Intraperitoneal injection of DT ablates monocyte/macrophage cells in the peritoneal cavity. Unmanipulated CD11b/DTR NOD mice are viable, normal in size and develop spontaneous diabetes similar to NOD/ShiLtJ. Transgene expression of EGFP is not sufficient to be detected by FACS analysis. Twelve hours following DT administration all macrophages are ablated in the spleen and lymph nodes, including the pancreatic lymph node and pancreas for three to five days. Macrophage population is restored by day four following a ..... For more information please see the full phenotype on the strain data sheet | ||
| 008694 | NOD/ShiLt-Tg(Foxp3-EGFP/cre)1cJbs/J | Repository- Live |
| Mice hemizygous for the Foxp3-EGFP/cre BAC transgene are viable and fertile. They express an enhanced green fluorescent protein and codon optimized "humanized" cre under the control of the mouse Foxp3 promoter. Transgene expression is specific to Cd4+Cd25 highCd127 low T cells from the lymph nodes, spleen and thymus. GFP expression is restricted to FoxP3+ cells. This mutant mouse strain may be useful for fluorescently labeling Foxp3+ T-cells to study regulatory T-cell function in autoimmunity specifically, type 1 diabetes. | ||
| 002364 | STOCK Cftrtm1Unc Tg(FABPCFTR)1Jaw/J | Repository- Live |
| These FABP-hCFTR-CFTR bitransgenic mice harbor the FABP-hCFTR transgene (human fatty acid binding protein 1 liver (FABP1) promoter directing expression of a human cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) (CFTR) gene) and a targeted mutation of the cystic fibrosis transmembrane conductance regulator homolog gene (Cftrtm1Unc). Mice homozygous for both the Cftr targeted mutation and the FABP-hCFTR transgene have normal longevity up to nine months (longest time examined). There is correction of ileal goblet cell and crypt cell hyperplasia and cAMP-stimulated chloride secretion. There is little or no expression of the transgene in the lung. This more robust model may be used to assess the effects of the null mutation on the nose and lungs, and may be useful as a mouse model of Cystic Fibrosis.
Of note, colonies maintained at The Jackson Laboratory report poor breeding performance when using ..... | ||
| 010963 | STOCK Fxntm1Mkn Tg(FXN)YG22Pook/J | Repository- Live |
| The YG22 transgenic founder line carries a single copy of the human FXN gene with one GAA trinucleotide repeat sequence of 190 repeats. High levels of human FXN gene product (mRNA or protein) are detected by RT-PCR and Western blot analysis. 40-50% of the endogenous mouse Fxn gene product (protein) is detected by Western blot analysis in mice heterozygous for the targeted mutation alone. Mice that are homozygous for the targeted allele and hemizygous for the transgene exhibit an age dependent, tissue specific expansion of the GAA repeat, with expansion accumulation in the CNS (particularly cerebellum), similar to the human pathology. The GAA triplet repeat exhibits intergenerational instability. Mice that are homozygous for the targeted allele and hemizygous for the transgene exhibit progressive retinal degeneration, impaired and decreased locomotor activity and coordination, and an increase in body weight. At 9 months of age, muscle strength is decreased. Alt ..... For more information please see the full phenotype on the strain data sheet | ||
| 008398 | STOCK Fxntm1Mkn Tg(FXN)YG8Pook/J | Repository- Live |
| Mice that are heterozygous for the targeted allele and hemizygous for the transgene are viable and fertile. High levels of human FXN gene product (mRNA or protein) are detected by RT-PCR and Western blot analysis. 40-50% of the endogenous mouse Fxn gene product (protein) is detected by Western blot analysis in mice heterozygous for the targeted mutation alone. The YG8 transgenic founder carries two tandem copies of the human FXN gene with two GAA triplet repeat sequences of 82 and 190 repeats. Mice that are homozygous for the targeted allele and hemizygous for the transgene exhibit an age dependent, tissue specific expansion of the GAA repeat, with expansion accumulation in the CNS (particularly cerebellum), similar to the human pathology. The GAA triplet repeat exhibits intergenerational instability.
This mutant mouse strain may be useful in studies of Friedreich's Ataxia. Importation of this model was supported by the Friedreichs Ataxia Research All ..... | ||
| 013124 | STOCK Gt(ROSA)26Sortm3(Gli3)Amc/J | Repository- Live |
| These RosaGli3TFlag c/c mice contain a floxed-neomycin resistance (neo) cassette and polyadenylation signal, cDNA encoding a FLAG-tagged GLI-Kruppel family member (Gli3) repressor gene, an internal ribosome entry site (IRES), and a Venus yellow fluorescent protein (YFP) under control of the ubiquitous Gt(ROSA)26Sor locus. Breeding these mutant mice to mice that express Cre-recombinase will also result in Floxed-neo-stop excision. When these mice are crossed to mice containing Cre-recombinase under direction of a paired related homeobox 1 (Prrx1) promoter (see Stock No. 005584), active in early limb mesenchyme, the mice produce Gli3TFlag at levels that are comparable with the endogenous protein. Mice exhibited a variety of limb defects including a variable preaxial forelimb polydactyly, limb truncation, and reduced mineralization. These mice may be useful for understanding Sonic hedgehog signaling and iden ..... For more information please see the full phenotype on the strain data sheet | ||
| 013123 | STOCK Gt(ROSA)26Sortm6(Gli1)Amc/J | Repository- Live |
| These RosaGli1Flag c/c mice contain a floxed-neomycin resistance (neo) cassette and polyadenylation signal, cDNA encoding a FLAG-tagged GLI-Kruppel family member (Gli1) gene, an internal ribosome entry site (IRES), and a Venus yellow fluorescent protein (YFP) under control of the ubiquitous Gt(ROSA)26Sor locus. Breeding these mutant mice to mice that express Cre-recombinase will also result in Floxed-neo-stop excision. When these mice are crossed to mice containing Cre-recombinase under direction of an atonal homolog 1 (Math1) promoter, active in dividing granule neuron precursor cells and medulloblastoma tumors, the mice produce Gli1Flag at levels higher than the endogenous protein in the cerebellum. These mice may be useful for understanding Sonic hedgehog signaling and identifying targets of Gli1 action in developing ventral neural tube. | ||
| 003342 | STOCK Hbatm1Paz Hbbtm1Tow Tg(HBA-HBBs)41Paz/J | Repository- Live |
| This strain was engineered so that it no longer expresses mouse Hba and Hbb, but does express human HBA and HBB. It mimics the genetic, hematologic and histopathologic features that are found in humans afflicted with sickle cell anemia, including irreversibly sickled red blood cells, anemia and multiorgan pathology. A significant percentage of sickle cell mice do not survive to adulthood. | ||
| 007022 | STOCK Mnx1tm4(cre)Tmj Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(SMN2)89Ahmb/J | Repository- Live |
| Mice homozygous for the Tg(SMN2*delta7)4299Ahmb and Tg(SMN2)89Ahmb transgenes and the Smntm1Msd targeted mutation allele exhibit symptoms and neuropathology similar to patients afflicted with severe proximal spinal muscular atrophy (SMA), and a similar phenotype observed in Stock no. 005025. At birth, triple mutants are noticeably smaller than normal littermates. By day 5, signs of muscle weakness are apparent and become progressively more pronounced over the following week as the mice display an abnormal gait, shakiness in the hind limbs and a tendency to fall over. In addition, this strain carries the Mnx1 For more information please see the full phenotype on the strain data sheet | ||
| 006770 | STOCK Rag1tm1Mom Tg(TIE2GFP)287Sato/J | Repository- Live |
| To generate this double mutant strain, B6.Cg-Tg(TIE2GFP)287Sato/1J (Stock No. 004659) was crossed to C.129S7(B6)-Rag1tm1Mom/J (Stock No. 003145). This mutant mouse strain may be useful in studies examining angiogenesis in transplanted tissues. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described for each single mutant. We will modify the strain description if necessary as published results become available. | ||
| 008212 | STOCK Smn1tm1Msd Tg(Prnp-SMN)92Ahmb Tg(SMN2)89Ahmb/J | Repository- Live |
| As described for SMA mice (see Stock No. 005024), mice homozygous for Smn1tm1Msd targeted mutation (Smn null allele) and human SMN2 transgene (SMN2 low copy line 89) exhibit symptoms, neuropathology, and early lethality similar to human type I proximal spinal muscular atrophy (SMA) patients. As an addition to that SMA model, this strain also carries the PrP-SMN transgene; with the mouse prion protein (PrP or Prnp) promoter directing full-length human SMN expression at high levels in neurons (with low expression in skeletal muscle and liver). When the PrP-SMN transgene is derived from PrP92-SMN founder mice, high SMN expression in spinal cord and brain is observed. Homozygous SMN2; Smn; Prp92-SMN mice are rescued from the severe SMA phenotype, have significantly increased lifespan (average of 210 days) and have normal lumbar motor neuron root counts. Homozygous SMN2; Smn; PrP92-SMN mal ..... For more information please see the full phenotype on the strain data sheet | ||
| 007951 | STOCK Smn1tm3(SMN2/Smn1)Mrph Tg(SMN2*delta7)4299Ahmb Tg(SMN2)89Ahmb/J | Repository- Live |
| This triple mutant mouse harbors two transgenic alleles and a single targeted mutation. The Tg(SMN2*delta7)4299Ahmb allele consists of a human SMN2 (survival of motor neuron 2, centromeric) cDNA lacking exon 7 whereas the Tg(SMN2)89Ahmb allele consists of the entire human SMN2 gene. Mice that are homozygous for the targeted mutant Smn1tm3(SMN2/Smn1/SMN2)Mrph allele and homozygous for the two transgenic alleles should function similarly to SMA mutant strain FVB.Cg-Tg(SMN2*delta7)4299Ahmb Tg(SMN2)89Ahmb Smn1tm1Msd/J (Stock No. 005025). The targeted mutant Smn1tm3(SMN2/Smn1/SMN2)Mrph allele is engineered to revert to a fully functional Smn1 allele upon Cre-mediated recombination. This mutant mouse strain may be useful in studies of Spinal Muscular Atrophy.
Importation of this model was supported by the Spinal Muscular Atrophy Foundation. | ||
| 012620 | STOCK Trp53tm1Brd Brca1tm1Aash Tg(LGB-cre)74Acl/J | Repository- Live |
| BLG-Cre; Brca1F22-24/F22-24; p53+/- mice carry the beta-lactoglobulin (BLG)-Cre transgene, are homozygous for floxed exons 22-24 of the breast cancer 1 (Brca1) allele, and are heterozygous for p53 tumor-suppressor gene (p53) deficiency. Mice of this genotype are viable, fertile, normal in size and do not display any behavioral abnormalities. BLG-Cre; Brca1F22-24/F22-24; p53+/- females have expression of the BLG-Cre transgene during lactation; which leads to loss of Brca1 function in the mammary gland. This results in formation of mammary tumors exhibiting high grade central necrosis and metaplastic elements in the form of spindle cell and squamous cell differentiation; as seen in human basal-like breast cancers and BRCA1 mutation carriers. Heterozygosity for the mutant p53 allele accelerates the formation of mammary tumors. This strain may be useful for studying human basal-like cancer and breast ..... For more information please see the full phenotype on the strain data sheet | ||
| 008813 | STOCK Trpa1tm2Kykw Tg(CAG-cre/Esr1*)5Amc/J | Repository- Live |
| These compound mutant mice carry a floxed allele of the Trpa1 (transient receptor potential cation channel, subfamily A, member 1) gene in addition to a tamoxifen inducible cre transgene driven by the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer (see Stock No. 004682). Restricted to the cytoplasm, the cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. Tamoxifen administration induces cre recombination within the Trpa1 allele, excising the S5/S6 transmembrane domains. Animals not treated with tamoxifen display no observable phenotype. | ||
| 013749 | STOCK Tg(ACTB-EGFP,-tdTomato)11Luo/J | Repository- Live |
| Homozygous MADM-11GT mice are viable and fertile with no gross behavioral or observable abnormalities. The MADM-11GT allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP), a beta-globin intronic sequence containing an frt site and a loxP-flanked neomycin resistance gene, and the MYC-tagged C-terminal portion of a red fluorescent protein (tdTomato) all inserted into the Hipp11 locus on chromosome 11 (cytoband A1 at ~3cM between the Eif4enif1 and Drg1 loci). These MADM-11GT mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to MADM-11TG mice harboring a reciprocal mutation at the same locus (see Stock No. 013751). The resulting GT/TG offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygou ..... For more information please see the full phenotype on the strain data sheet | ||
| 014092 | STOCK Tg(ACTB-tTA2,-MAPT/lacZ)1Luo/J | Repository- Live |
| Homozygous CAG-stop-tTA2 transgenic mice are viable and fertile. CAG-stop-tTA2 transgenic mice harbor the ii-CAG-stop-tTA2-IRES-tau-lacZ-ii transgene; designed with a loxP-flanked transcriptional STOP cassette preventing transcription of the downstream modified tetracycline-regulated transactivator (tTA2). The ii-CAG-stop-tTA2-IRES-tau-lacZ-ii transgene is flanked by two copies of the chicken β-globin HS4 insulator on each side to preserve expression fidelity (see additional information below). When bred to mice that express a tamoxifen-inducible Cre recombinase (CreERT2), administration of tamoxifen to the double mutant offspring allows the CreERT2 fusion protein to enter the nucleus of the cre-expressing cells; this deletes the STOP cassette and results in expression of tTA2. The donating investigator reports that tau-lacZ fusion protein expression in the tamoxifen-treated double mutant offspring is faint. Of note, the donating investigator h ..... For more information please see the full phenotype on the strain data sheet | ||
| 013751 | STOCK Tg(ACTB-tdTomato,-EGFP)11Luo/J | Repository- Live |
| Homozygous MADM-11TG mice are viable and fertile with no gross behavioral or observable abnormalities. The MADM-11TG allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of a red fluorescent protein (tdTomato), a beta-globin intronic sequence containing an frt site and a loxP-flanked neomycin resistance gene, and the C-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP) all inserted into the Hipp11 locus on chromosome 11 (cytoband A1 at ~3cM between the Eif4enif1 and Drg1 loci). These MADM-11TG mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to MADM-11GT mice harboring a reciprocal mutation at the same locus (see Stock No. 013749). The resulting TG/GT offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous"), and mu ..... For more information please see the full phenotype on the strain data sheet | ||
| 014177 | STOCK Tg(Afp-mCherry)1Hadj/J | Repository- Live |
| Afp::mCherry transgenic mice have the alpha fetoprotein (Afp) promoter/enhancer sequences driving expression of a monomeric red fluorescent protein, mCherry. Hemizygotes are viable, fertile, and normal in size. Under control of Afp, mCherry labels the visceral endoderm and its derivatives, including the visceral yolk sac and gut endoderm. Expression is also seen in fetal liver and pancreas, and in liver, pancreas, digestive tract and brain of postnatal mice. These mice may be useful as a bright and photostable means for visualizing morphogenesis and tissue rearrangements in the developing embryo. | ||
| 007684 | STOCK Tg(Atoh1-cre/Esr1*)14Fsh/J | Repository- Live |
| Mice hemizygous for this Math1-CreERT2 transgene are viable and fertile. Under control of the Math1 (Atoh1) enhancer, tamoxifen-inducible cre activity is observed in neural progenitors of the cerebellar rhombic lip, dorsal hindbrain and spinal cord, as well as in inner-ear primordia (with a limited amount of ectopic expression in the primordium of the hippocampus but not the cortex). The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone ma ..... For more information please see the full phenotype on the strain data sheet | ||
| 003920 | STOCK Tg(CAG-Bgeo/GFP)21Lbe/J | Repository- Live |
| These Z/EG transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being liver and lung tissue. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with enhanced GFP expression in tissues expressing cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess cre excision activity in live animals and cells. Although homozygotes are viable, attempts to breed homozygous mice proved unsuccessful. | ||
| 005441 | STOCK Tg(CAG-DsRed*MST)1Nagy/J | Repository- Live |
| Mice homozygous for this Actb-DsRed.T3 transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the red fluorescent protein variant DsRed.MST under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. All tissues of homozygotes fluoresce red. Mice hemizygous for the transgene express red fluorescent protein less intensely than homozygotes. Expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle. | ||
| 003116 | STOCK Tg(CAG-EGFP)D4Nagy/J | Repository- Live |
| This transgenic strain carries the Enhanced Green Fluorescent Protein (Clontech) driven by chicken beta-actin promoter and CMV intermediate early enhancer. The transgene was found to have integrated on the X chromosome. Mice of this strain, and cells derived from them, can be distinguished from wildtype on the basis of fluorescence. Transgene expression is seen as early as ~embryonic day 2.75, as morula stage embryos begin to compact. Expression of EGFP is spatiotemporally widespread, though levels differ between different lineages (e.g., expression is slightly higher in the heart). As the transgene is located on the X chromosome, transgenic males will exclusively transmit the EGFP transgene to their female offspring, providing the basis for a non-invasive sexing assay based on green fluorescence. These mice may be useful for sex discrimination prior to overt sexual dimorphism (embryonic day 12.5), monitoring X-inactivation, and may be a source of fluorescently marked cells/tiss ..... For more information please see the full phenotype on the strain data sheet | ||
| 011106 | STOCK Tg(CAG-GFP*)1Hadj/J | Repository- Live |
| Mice harboring the lipid modified CAG-GFP* transgene are viable and fertile, normal in size and do not display any gross physical or behavioral abnormalities. The CAG promoter directs widespread expression of a glycosylphosphatidylinositol-tagged (GPI) green fluorescent protein (GFP) fusion throughout embryonic development and adulthood. Expression is targeted to the plasma membrane, Golgi membranes and some secretory vesicles. Expression is enriched in the apical regions of the plasma membrane. Hemizygotes and homozygotes exhibit identical distribution patterns, however, homozygotes exhibit increased fluorescence. This mutant mouse strain may be useful for in vivo imaging of cell morphology, plasma membrane dynamics and lipid localization. | ||
| 013753 | STOCK Tg(CAG-KikGR)33Hadj/J | Repository- Live |
| CAG::KikGR33 transgenic mice express a Kikume Green-Red (KikGR) photoconvertible fluorescent protein under the control of a CMV enhancer/chicken beta-actin promoter (CAGGS) promoter. Mice homozygous for the transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. KikGR, engineered from Favia favus coral, changes color from green to red upon activation in embryos, adult mice, and embryonic stem (ES) cells. At basal state, green fluorescence is seen in all cells. A single cell or group of cells at basal state, exposed to 405 nm wavelength light, undergo photo conversion and fluoresce red. Since KikGR is developmentally neutral and non-toxic, the movement of these fluorescent cells, and their progeny, can be imaged during embryonic development. Mice from founder line 33 exhibits widespread expression of KikGR, while mice from founder line 75 (Stock No. ..... For more information please see the full phenotype on the strain data sheet | ||
| 009678 | STOCK Tg(CAG-RAB9A)500Repa/J | Repository- Live |
| These transgenic mice express HA-tagged human RAB9A, member RAS oncogene family, gene (RAB9A) under the control of the CAG promoter (CAGGS, chicken beta actin promoter/enhancer coupled with the cytomegalovirus immediate-early enhancer). Transgene expression is detected in liver, brain, kidney, and skin, and is highest in brain and liver. Expression is approximately 30-fold higher than endogenous RAB9 protein in the liver. Mice that are hemizygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. This mutant mouse strain may be useful in studies of Niemann-Pick, type C (NP-C) disease.
Double mutant mice that carry this transgene and are homozygous for the Npc1m1N allele (see Stock No. 003092) exhibit a less severe phenotype than mice homozygous for t ..... | ||
| 003273 | STOCK Tg(CMV-rtTA)4Bjd/J | Repository- Live |
| Homozygous transgenic mice are viable, fertile, and display no overt phenotype. These mice carry the gene encoding the reverse tetracyline-controlled transactivator protein (rtTA) under the control of a human cytomegalovirus early promoter (PhCMV). When these mice are mated to a strain carrying a luciferase reporter gene coupled to a tetracycline-responsive promoter element (TRE; tetO), luciferase expression in the bitransgenic offspring is induced up to 105-fold by treatment with doxycycline (dox) in organs and tissues where PhCMVis known to be active, (i.e. muscle, kidney, thymus and pancreas). Little to no luciferase expression is induced in tissues where PhCMV is not active (i.e. lung, brain, liver and lymphocytes). These mice may be mated to strains containing a gene of interest coupled to a TRE to study the target gene expression effects under tissue-specific, dox-inducible regulation. Dox may be administered in the animals? water suppl ..... For more information please see the full phenotype on the strain data sheet | ||
| 009615 | STOCK Tg(Cartpt-cre)1Aibs/J | Repository- Live |
| Hemizygous Cart-Tg1-Cre mice are viable and fertile, with cre expression directed to cortex, hippocampus, and cerebellum by the Cartpt promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Cart-Tg1-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex, hippocampus, and cerebellum).
For characterization information, see images at the Allen Institute for Brain Science website (Cart-Tg1-Cre images). | ||
| 008241 | STOCK Tg(Cspg4-DsRed.T1)1Akik/J | Repository- Live |
| Mice hemizygous for the NG2DsRedBAC transgene are viable and fertile, expressing an optimized red fluorescent protein variant (DsRed.T1) under the control of the mouse NG2 (Cspg4) promoter/enhancer. DsRed.T1 fluorescence is detected in NG2 cells (oligodendrocyte progenitor cells) throughout the postnatal central nervous system, including the gray and white matter of the brain, cerebellum and spinal cord, and vascular mural cells; but not in mature oligodendrocytes, astrocytes, resting microglia, or neurons. DsRed.T1 fluorescence intensity is highest in cell bodies and can also be detected in distal processes, suggesting that tetrameric DsRed.T1 remains soluble and is not toxic to cells. In addition, DsRed.T1 fluorescence may be readily detected without using anti-DsRed antibodies and is suitable for identifying NG2 cells in live slices or for purifying NG2 cells via FACS. These NG2DsRedBAC transgenic mice may be useful for fluorescent labeling of NG2 cells (oligodendrocyte p ..... For more information please see the full phenotype on the strain data sheet | ||
| 003208 | STOCK Tg(DR4)1Jae/J | Repository- Live |
| MEFs prepared from the DR-4 mouse strain displayed resistance to concentrations of the drugs G418, 6-thioguanine, puromycin and hygromycin well above those used normally for the selection of drug-resistant ES cells. This mouse strain represents an economical donor for the production of multiple-drug resistant mouse embryonic fibroblasts (MEFs). | ||
| 008861 | STOCK Tg(Ela1-Cre/ERT2)1Stof/J | Repository- Live |
| These transgenic mice have a tamoxifen-inducible, Cre-mediated recombination system driven by the rat elastase 1, pancreatic (Ela1) promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the human estrogen receptor ligand binding domain (CreERT2). The mutant human estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/ESR1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. No cre activity is detectable without tamoxifen treatment. When these transgenic mice are bred with mice containing a loxP flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Ela1
expressing cells; making them useful in fate mapping of pancreatic acinar cells.
Hemizygous mutant mice are viable, fertile, normal in ..... For more information please see the full phenotype on the strain data sheet | ||
| 005938 | STOCK Tg(Eno2-cre)39Jme/J | Repository- Live |
| Mice hemizygous for this NSE39-Cre transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These NSE39-Cre mice harbor a transgenic insert consisting of the cre recombinase gene under the control of the promoter region of the rat neuron specific enolase (NSE or Eno2) gene. As such, Cre recombinase activity is directed to neurons with expression in many tissue types. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome.
Specifically, these NSE39-Cre transgenic mice may also be useful in studies of spinal muscular atrophy (SMA) along with mice harboring a conditional (floxed) Smn1 gene (see Stock No. 006138 or Stock No. 006146).
Additional SMA strains expressing cre in striated muscle are av ..... For more information please see the full phenotype on the strain data sheet | ||
| 004623 | STOCK Tg(Fos-lacZ)34Efu/J | Repository- Live |
| These TOPGAL transgenic mice are a reporter strain that express Beta-galactosidase in the presence of the lymphoid enhancer binding factor 1/transcription factor 3 (LEF/TCF) mediated signaling pathway and activated Beta-catenin. The transgene contains the lacZ gene under the control of a regulatory sequence consisting of three consensus LEF/TCF-binding motifs upstream of a minimal c-fos promoter. Transgenic mice display TOPGAL activity (Beta-galactosidase activity) during early embryonic development in a subset of pluripotent embryonic basal cells of the epithelium and dermis of developing hair follicles, but not during the next stage of hair follicle development; formation of hair germs. TOPGAL transgene activity reappears in hair follicles at E16.5 and TOPGAL expression is strongly upregulated in the postnatal hair shaft precursor cells in both whisker and body hair anagen follicles (active periods of hair growth). TOPGAL expression ceases during catagen (regression and ..... For more information please see the full phenotype on the strain data sheet | ||
| 011062 | STOCK Tg(Gdf9-cre)5092Coo/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse growth differentiation factor 9 (Gdf9) promoter. Cre recombinase expression is detected in oocytes of the primordial follicles by postnatal day 3 and in oocytes, but not somatic cells, of all follicles at the primary, secondary and later stages by 24 days. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in the offspring. This mutant mouse strain may be useful in studies of studies of folliculogenesis and oocyte development. | ||
| 012841 | STOCK Tg(Ggt1-cre)M3Egn/J | Repository- Live |
| These transgenic mice express Cre recombinase under the control of the rat Ggt1, gamma-glutamyltransferase 1, promoter. Cre recombinase expression is detected by Northern blot in the kidney beginning at age 7 days. No transcript was detected in brain, liver, spleen, muscle, lung or adrenal gland. Cre recombinase protein is detected immunohistochemically in cortical proximal tubules. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the cortical tubular epithelium. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator reports that homozygotes are viable and fertile. | ||
| 005418 | STOCK Tg(HIST1H2BB/EGFP)1Pa/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express an "H2B-EGFP" fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Green Fluorescent Protein gene [EGFP, BD Biosciences]) under the control of the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer. Nucleosomes and chromatin in all cells fluoresce. Fluorescence is detectable during all phases of mitosis. The donating investigator reports occasional silencing of the transgene when transmitted through the female germ line. This mutant mouse strain may be useful for in vivo subcellular high-resolution and 3-dimensional studies of dividing cells. | ||
| 016252 | STOCK Tg(Hoxb7-Venus*)17Cos/J | Repository- Live |
| Hoxb7-myr-Venus transgenic mice have the homeobox B7 promoter/enhancer sequences driving expression of a myristoylated yellow fluorescent protein, myr-Venus. Hemizygotes are viable, fertile, and normal in size. Under control of Hoxb7, myr-Venus labels the membranes of individual cells in the Wolffian duct and branching ureteric bud (UB) of the kidney during all stages of urogenital development. This strain allows for the visualization of the shapes and arrangements of individual cells. In contrast, mice containing the Tg(Hoxb7-EGFP)33Cos allele (Stock No. 016251) are useful for viewing tissues specific to the UB lineage. | ||
| 014600 | STOCK Tg(I12b-cre/ERT2,-ALPP)37Fsh/J | Repository- Live |
| These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse Dlx1/2, distal-less homeobox 1, distal-less homeobox 2, forebrain enhancer/ promoter (I12b). The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain, and the human placental alkaline phosphatase (ALPP or PLAP). The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/ESR1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing loxP site flanked sequences, the flanked sequences will be deleted in the cre-expressing tissues in the offspring upon administration of tamoxifen. Tamoxifen administration induces Cre recombination in the ventral telencephalon and diencephalon as early as em ..... For more information please see the full phenotype on the strain data sheet | ||
| 008755 | STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J | Repository- Live |
| This strain was generated by breeding Stock No. 008168 and Stock No. 008250 together at The Jackson Laboratory. The resulting double transgenic colony was established as Stock No. 008755. The Ins2-rtTA (or RIP7-rtTA) transgene expresses the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat insulin 2 (Ins2) promoter. The tet-DTA (or tetO-DTA) (transgene expresses diphtheria toxin A (DTA) under the control of a tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) and a cytomegalovirus minimal promoter. Mice harboring both of these transgenes has doxycycline-inducible expression of DTA in pancreatic beta cells; i.e. addition of the tetracycline analogue doxycycline (dox) results in ablation of pancreatic beta cells. | ||
| 004782 | STOCK Tg(KRT14-cre)1Amc/J | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the Cre recombinase under the control of the human keratin 14 promoter. Cre transcript is detected in the skin. When crossed to a reporter line containing Gt(ROSA)26Sortm1Sor, Beta-galactosidase activity is detected in the oral ectoderm at 11.75 dpc, and at 14.5 dpc activity is detected in the skin and throughout the dental epithelium. This strain represents an effective tool for generating tissue-specific targeted mutants that would be useful to study developmentally critical gene function in the ectoderm and its derivatives.
View cre expression characterization. | ||
| 005107 | STOCK Tg(KRT14-cre/ERT)20Efu/J | Repository- Live |
| These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the human keratin 14 (KRT14) promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen (tamoxifen). Restricted to the cytoplasm, the cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted keratinocyte-specific deletions. Oral tamoxifen administration induces Cre recombination in toe, back skin and tongue. Topically administered tamoxifen induces Cre-mediated recombination in a specific localized area of the skin, occuring in 50 to 60% of the ..... For more information please see the full phenotype on the strain data sheet | ||
| 003553 | STOCK Tg(MMTV-cre)4Mam/J | Repository- Live |
| This transgenic strain expresses P1 Cre recombinase under the control of the MMTV LTR promoter. The MMTV LTR promoter directs a widespread pattern of expression. High levels of recombination have been detected in the virgin and lactating mammary gland, salivary gland, seminal vesicle, skin, erythrocytes, B and T cells. Little background recombination was observed in the lung, kidney, liver and brain tissues (less than 10%). The donating investigator indicates that this strain may be suitable for use in applications where it is desirable to delete genes in the virgin and lactating mammary gland, skin, erythroid cells, and other secretory tissues and skin. | ||
| 009075 | STOCK Tg(Myh6-Ppp3ca)37Eno/J | Repository- Live |
| Calcineurin A (Ppp3ca) expression is driven by the cardiomyocyte-specific alpha myosin heavy chain (alpha MHC) promoter in this transgenic strain. Mice develop cardiac hypertrophy that progresses to dilated cardiomyopathy with interstitial fibrosis, congestive heart failure and sudden death between 6 and 12 months of age. This strain may be useful as a model of human heart disease. | ||
| 009074 | STOCK Tg(Myh6-cre)1Jmk/J | Repository- Live |
| These transgenic mice express nuclear-localizing Cre recombinase under the control of the mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha; alpha-MHC) promoter. Cre recombinase expression is detected in a majority of cardiac myocytes. Approximately 70% efficiency has been demonstrated when crossed with one floxed allele strain. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Evidence suggests that this transgene is X-linked. | ||
| 005650 | STOCK Tg(Myh6-cre/Esr1*)1Jmk/J | Repository- Live |
| The alpha-MHC-MerCreMer transgene has the mouse Myh6 promoter (myosin, heavy polypeptide 6, cardiac muscle, alpha; alpha-MHC) directing expression of a tamoxifen-inducible Cre recombinase (MerCreMer) to juvenile and adult cardiac myocytes. Mice homozygous for the alpha-MHC-MerCreMer transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre recombinase expression in heart tissue is confirmed by western blot. Southern blot confirmed heart cell specificity compared to brain, kidney, lung, liver, and skeletal muscle. Insertion of this transgene and its protein show no changes in echocardiography, heart mass or pathology, or hypertrophy marker genes compared to nontransgenic littermates. Of note, the MerCreMer double fusion protein has substantially greater Cre recombinase activity with less promiscuity compared with the CreMer single fusion protein. When alpha-MHC-MerCreMer transgenic mice are bred with mice containing For more information please see the full phenotype on the strain data sheet | ||
| 012462 | STOCK Tg(Nr5a1-cre)7Lowl/J | Repository- Live |
| These transgenic mice express Cre recombinase under the direction of the mouse Nr5a1, nuclear receptor subfamily 5, group A, member 1, promoter. The Cre recombinase activity pattern mimics the endogenous gene expression (mRNA) pattern. Cre mediated recombination is detected in steroidogenic factor-1 (SF1)-positive neurons in the ventromedial hypothalamic nucleus (VMH) as well as pituitary, gonad, and adrenal tissue. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in tissue-specific deletion of the flanked sequence in the offspring. | ||
| 014158 | STOCK Tg(Pax4-cre)1Dam/J | Repository- Live |
| Pax4-cre transgenic mice have the mouse paired box gene 4 (Pax4) promoter directing expression of an enhanced green fluorescent protein fused to a Tet-off cassette (EGFP/tTA). The transgene also contains a tetracycline-responsive element with a CMV minimal promoter (tetO-CMVmin) driving expression of a Cre-recombinase gene. In the absence of tetracycline/doxycycline, Cre recombinase expression is observed in Pax4-expressing cells (pancreatic progenitor cells). While designed to concomitantly allow tetracycline/doxycycline-dependant inhibition of Cre recombinase expression, the donating investigator confirms no such inhibition is observed. Also, no GFP expression is observed with or without tetracycline/doxycycline administration. Therefore, Pax4-cre transgenic mice function only for Cre recombinase expression in Pax4-expressing cells. When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of ..... For more information please see the full phenotype on the strain data sheet | ||
| 014099 | STOCK Tg(Pmch-cre)1Lowl/J | Repository- Live |
| Mch-cre transgenic mice have the murine pro-melanin-concentrating hormone (Mch) promoter/enhancer regions within the BAC transgene directing expression of Cre-recombinase. Hemizygous Mch-cre mice are viable, fertile, and normal in size, with cre expression directed specifically to MCH-neurons of the hypothalamus and zona incerta. When these transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing neurons of the offspring. MCH neurons regulate many physiological functions by projecting through the brain. These mice may be useful for studying MCH-neuronal activities in controlling energy balance, glucose homeostasis, sleep, locomotion, and reward-related behaviors. | ||
| 005965 | STOCK Tg(Pomc1-cre)16Lowl/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is demonstrable in brain area neurons involved in the control of food intake (arcuate nucleus (hypothalamus) and solitary tract nucleus (hindbrain)). When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked genome in tissues that normally express Pomc1. The mice may be useful in studies of obesity, food intake, hunger, endocrine and exocrine function, and for tissue specific gene targeting. | ||
| 008477 | STOCK Tg(RARE-Hspa1b/lacZ)12Jrt/J | Repository- Live |
| These transgenic mice express beta-galactosidase (lacZ) gene under the control of the retinoic acid responsive element (RARE). Sporadic beta-galactosidase activity is detected in less than half of embryonic day 3.5 blastocysts. Beta-galactosidase activity in embryos 11.5 embryonic days in age and older mimics the expression pattern of retinoic acid receptor, beta (Rarb). Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When maintained as homozygotes, this strain can lose beta-galactosidase staining capacity. The donating investigator reports that lacZ staining is lost when their mice are bred onto the C57BL/6 genetic background. This strain serves as a reporter strain, with retinoic acid signaling being indicated by beta-galactosidase activity. This mutant mouse strain may be useful in tracking retinoic acid signaling pattern. | ||
| 009606 | STOCK Tg(Six2-EGFP/cre)1Amc/J | Repository- Live |
| Hemizygous Six2-TGCtg mice are viable and fertile, harboring a BAC transgene with a Tet-off-eGFPCre under control of the Six2 promoter/enhancer regions within the BAC transgene. The Tet-off-eGFPCre contains both the tetracycline-controlled transactivator protein (tTA) as well as the tetracycline operator (tetO; also called tetracycline-responsive element [TRE] or tet-operator) upstream of an EGFPCre fusion protein. Sequence updates published after this transgene was created revealed an upstream Six2 ATG start site that is in-frame with the tTA; thus an apparent and unintended fusion was created with the tTA (80 amino acids potentially added) that renders transgene expression unaffected by tetracycline/doxycycline administration. It is not known whether the "mutant" tTA is resistant to tetracycline/doxycycline inactivation and still binds to TRE to activate EGFPCre expression or if the TRE acts as a minimal promoter adjacent to the Six2 promoter in the pr ..... For more information please see the full phenotype on the strain data sheet | ||
| 012586 | STOCK Tg(Slc1a3-cre/ERT)1Nat/J | Repository- Live |
| This BAC transgenic line expresses CreERT under the control of the Slc1a3 (solute carrier family 1 (glial high affinity glutamate transporter); GLAST) promoter. When crossed with a strain containing a loxP site flanked sequence, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted glia and neural progenitor cell-specific deletions. Injection of 4-hydroxytamoxifen leads to recombination specifically in Muller glia in the retina, in astrocytes of the brain, and in neural progenitors, including those that give rise to hippocampal neurons and olfactory neurons in the rostral migratory stream. This strain is a useful tool in studies of neurodevelopment. | ||
| 004783 | STOCK Tg(Sox2-cre)1Amc/J | Repository- Live |
| Mice hemizygous for the Sox2Cre transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse SRY-box containing gene 2 promoter. When these transgenic mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in Sox2-expressing tissues in the offspring. Specifically, Cre recombinase activity is detected in the epiblast cells at embryonic day 6.5, with little or no activity in other cells at gastrulation. Some activity is also detected in extra embryonic derivatives of the epiblast, the yolk sac mesoderm and amnion. No Cre recombinase activity is detected in primitive endoderm derived tissues, visceral endoderm. The phenotype of homozygous mice has not been characterized to date (April 2011). These Sox2Cre transgenic may be useful for generating epiblast-derived specific ..... For more information please see the full phenotype on the strain data sheet | ||
| 008208 | STOCK Tg(Stra8-cre)1Reb/J | Repository- Live |
| Homozygous Stra8-cre transgenic mice are viable and fertile, with expression of an optimized variant of Cre recombinase (iCre) directed by the 1.4 kb mouse Stra8 (stimulated by retinoic acid gene 8) genomic promoter fragment. Postnatal Cre-recombinase expression is first observed in testes at postnatal day (P)3 (early-stage spermatogonia), increases out to P7 (pre-leptotene-stage spermatocytes), and is not detected in other tissues examined (including ovaries). In addition, no cre expression is reported in male or female embryos. When Stra8-cre transgenic males are bred with female mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence, specifically during these stages of spermatogenesis. These Stra8-cre transgenic mice may be useful in generating conditional knockouts in postnatal, premeiotic, male germ cells for studying spermatogenesis. | ||
| 013752 | STOCK Tg(TCF/Lef1-HIST1H2BB/EGFP)61Hadj/J | Repository- Live |
| TCF/Lef:H2B-GFP transgenic mice express an H2B-EGFP fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Green Fluorescent Protein gene) under the control of six copies of a T cell specific transcription factor/lymphoid enhancer-binding factor 1 (TCF/Lef1) response element and a heat shock protein 1B (Hspa1b) minimal promoter. Mice homozygous for the transgene are viable, fertile, and normal in size. Wnt (wingless-related MMTV) family members are required for triggering embryonic axis formation and for proper development. Wnt signaling results in phosphorylation and nuclear localization of β-catenin, a transcriptional co-activator protein, which, together with the TCF/Lef family of transcription factors, induces the transcription of downstream genes. TCF/Lef:H2B-GFP transgenic mice contain 6 copies of nuclear localized TCF/Lef1 DNA binding sites, which provides increased sensitivity to Wnt/&be ..... For more information please see the full phenotype on the strain data sheet | ||
| 003658 | STOCK Tg(TIE2GFP)287Sato/J | Repository- Live |
| This strain expresses Green Fluorescent Protein (GFP) under the direction of the endothelial-specific receptor tyrosine kinase (Tek, formerly, Tie2) promoter. Endothelial cells expressing GFP can be visualized via fluorescent microscopy or purified by FACS. | ||
| 004746 | STOCK Tg(Tagln-cre)1Her/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse transgelin (smooth muscle protein 22-alpha) promoter. Cre recombinase expression (mRNA) closely patterns endogenous transgelin expression, with the highest levels detected in the aorta, intestine and uterus. Low levels of transcript are detected in all other organs tested, likely reflecting the vascular smooth muscle compartments in the these tissues. Cre recombinase activity is observed in vascular smooth muscle cells of hepatic and pulmonary arteries, with no activity detected outside the vascular walls. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in vascular smooth muscle cells. This strain represents an effective tool for generating tissue specific-ta ..... For more information please see the full phenotype on the strain data sheet | ||
| 005493 | STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J | Repository- Live |
| Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice harbor co-injected transgenic constructs. The reverse tetracycline-controlled transactivator (rtTA) protein is expressed under the direction of the endothelial-specific receptor tyrosine kinase enhancer/promoter (Tek). The second transgenic construct expresses a nuclear-localizing beta-galactosidase gene (lacZ) under the control of a tetracycline-responsive element (TRE). In the presence of tetracycline or a tetracycline analog (such as doxycycline), selective expression of lacZ is observed in the nuclei of vascular endothelium in a wide variety of tissues (aorta, heart, brain, lung, kidney, liver, spleen, uterus, prostate, stomach, skeletal muscle, large and small intestine). Expression is observed as early as embryonic day 9.5. In the absence of tetracycline, some lacZ expression occurs in the smaller branches of ..... For more information please see the full phenotype on the strain data sheet | ||
| 013162 | STOCK Tg(Thy1-Clomeleon)12Gjau/J | Repository- Live |
| These transgenic mice express Clomeleon, a fluorescent fusion protein containing CFP and topaz YFP that acts as a ratiometric indicator for chloride ions, under the control of the mouse Thy1, thymus cell antigen 1, theta, promoter (Thy1.2). Transgene expression in founder line CLM12 is detected at high levels in the thalamus, cerebellar nuclei. No expression is detected in layers 1 and 2 of the neocortex, hippocampal regions CA2 and CA3, cerebellar purkinje cells, or retinal ganglion cells. In the presence of low chloride ion concentrations, YFP fluoresces as a FRET (Fluorescence Resonance Energy Transfer) acceptor for CFP. As the chloride ion concentration increases, YFP fluorescence decreases and CFP fluorescence increases. Using an appropriate calibration curve, the ratio of YFP and CFP fluorescence peaks indicate [Cl-]. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The ..... For more information please see the full phenotype on the strain data sheet | ||
| 013163 | STOCK Tg(Thy1-Clomeleon)13Gjau/J | Repository- Live |
| These transgenic mice express Clomeleon, a fluorescent fusion protein containing CFP and topaz YFP that acts as a ratiometric indicator for chloride ions, under the control of the mouse Thy1, thymus cell antigen 1, theta, promoter (Thy1.2). Transgene expression in founder line CLM13 is detected at high levels in the hippocampal dentate gyrus and CA1 region, amygdala, and nuclei and mossy fibers of the cerebellum. No expression is detected in the external plexiform layer, mitral cells or internal plexiform layer of the olfactory bulb, layer 1 of the neocortex, cerebellar granule or purkinje cells, or retinal biopolar and amacrine cells. In the presence of low chloride ion concentrations, YFP fluoresces as a FRET (Fluorescence Resonance Energy Transfer ) acceptor for CFP. As the chloride ion concentration increases, YFP fluorescence decreases and CFP fluorescence increases. Using an appropriate calibration curve, the ratio of YFP and CFP fluorescence peaks indicate [Cl-]. Mi ..... For more information please see the full phenotype on the strain data sheet | ||
| 007788 | STOCK Tg(Thy1-EGFP)MJrs/J | Repository- Live |
| Mice harboring the Thy1-GFP transgene are viable and fertile with enhanced green fluorescent protein (EGFP) expression under the control of a modified Thy1 promoter region (containing the sequences required for neuronal expression but lacking the sequences required for expression in non-neural cells). Homozygous or hemizygous Thy1-GFPM mice (derived from founder line M) express EGFP in sparse subsets of neurons within specific populations; providing a bright, vital Golgi-like stain. Less than 10% of all motor axons, retinal ganglion cells, lumbar dorsal root ganglion, and cortex express EGFP. These Thy1-GFPM transgenic mice may be useful in neurobiological studies for fluorescent labeling of neural tissues, especially for mossy fibers in the cerebellum and intense, yet sparse, labeling of a variety of neuronal subsets.
This strain is one of many from the donating investigator with specific/differential fluorescent protein expression in neural tissues (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 012708 | STOCK Tg(Thy1-cre/ERT2,-EYFP)HGfng/PyngJ | Repository- Live |
| The "single-neuron labeling inducible Cre-mediated knockout" (SLICK) transgene is designed with two separate copies of the modified mouse Thy1 promoter region (each containing the sequences required for neuronal expression but lacking the sequences required for expression in non-neural cells) driving expression of a CreERT2 fusion protein and an enhanced yellow fluorescent protein (EYFP). Mice harboring the SLICK transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. mRNA coexpression of both CreERT2 and YFP is highly correlated. YFP expression is directed primarily to projection neurons with variations in the extent and brightness of labeling observed in different founder lines (see below). CreERT2 fusion gene activity is inducible; observed at high levels following tamoxifen administration (see below). When SLICK transgenic mice are bred with mice containing loxP-flanked seque ..... For more information please see the full phenotype on the strain data sheet | ||
| 016584 | STOCK Tg(Tph2-icre/ERT2)6Gloss/J | Repository- Live |
| These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse Tph2, tryptophan hydroxylase 2, promoter. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in Tph2-expressing neurons in the raphe nucleus. Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
This Cre-ERT2 fusion protein consists of iCre recombinase (Codon-improved Cre recombinase) fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can onl ..... | ||
| 011108 | STOCK Tg(Ttr-RFP)1Hadj/J | Repository- Live |
| Mice hemizygous for the Ttr-RFP transgene are viable and fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice exhibit reduced viability on this background. Expression of the transgene is directed to the visceral endoderm of early postimplantation embryo (E5.5), yolk sac endoderm, and other endoderm-derived organs including intestine, liver, pancreas, and stomach. In addition, expression is detected in the optic vesicle and regional areas of the brain. This mutant mouse strain may be useful for in vivo imaging, fate mapping and genetic modification of cells of the visceral endoderm. | ||
| 003829 | STOCK Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J | Repository- Live |
| When homozygous for both co-injected transgenes, Wnt-1/GAL4/cre-11 transgenic mice are viable, fertile, normal in size and do not display any gross physical abnormalities. Both Cre recombinase and the GAL4 transcriptional activator are expressed under the direction of wingless-related MMTV integration site 1 (Wnt1) promoter/regulatory sequences. Cre recombinase activity is detected in the Wnt1 pattern of expression: in the midbrain by 8.5 dpc and, after neural tube closure, in the dorsal and ventral midlines of the midbrain and caudal diencephalon, midbrain-hindbrain junction and dorsal spinal cord. When Wnt-1/GAL4/cre-11 transgenic mice are bred to mice containing loxP site-flanked sequences, cre-mediated recombination results in deletion of the floxed sequences in the midbrain and developing neural tube of the resulting offspring. | ||
| 008199 | STOCK Tg(dlx6a-cre)1Mekk/J | Repository- Live |
| Homozygous Dlx5/6-Cre transgenic mice are viable and fertile. Expression of Cre recombinase (Cre) is directed to differentiating and migrating forebrain GABAergic neurons during embryonic development by the I56i and I56ii enhancers from the zebrafish dlx5a/dlx6a intergenic region (with the 5' promoter region of zebrafish dlx6a in place to increase the activity of the intergenic enhancers rather than direct tissue-specific expression). When Dlx5/6-Cre transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence in the offspring in the offspring. These Dlx5/6-Cre transgenic mice may be useful in generating specific deletions of floxed alleles in GABAergic forebrain neurons. | ||
| 005104 | STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J | Repository- Live |
| These transgenic mice express the human histone 1, H2bj, protein (HIST1H2BJ) and Green Fluorescent Protein (GFP) fusion protein, HIST1H2BJ/GFP, under the control of a tetracycline-responsive promoter element (TRE; tetO). Transgenic expression occurs in widespread fashion. When hemizygotes are bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) to create bitransgenic animals, tissue-specific HIST1H2BJ/GFP transgene expression can be regulated with the tetracycline analog, doxycycline. Pulse-chase administration of doxycycline results in retention of signal in rarely dividing, or infrequently cycling, label-retaining cells (LRCs). Potential constitutive transgene expression should be examined in tissues of interest. | ||
| 012345 | STOCK Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo/J | Repository- Live |
| Hemizygous TRE-Bi-SG-T line 1.1 transgenic mice are viable and fertile, with no reported phenotypic abnormalities. The TRE-Bi-SG-T transgene has Myc-tagged tdTomato and full-length mouse synaptophysin/mut4EGFP fusion protein (Syp-GFP) expression under the control of the bi-directional tet-responsive promoter (tetO or TRE). When bred with another mouse expressing tetracycline-controlled transactivator protein (tTA) or reverse tetracycline-controlled transactivator protein (rtTA), tdTomato and Syp-GFP fusion protein expression can be regulated with the tetracycline analog doxycycline (dox) in the resulting double mutant offspring. In tTA(-dox) or rtTA(+dox)-expressing cells, tdTomato expression is directed to the entire cell, while GFP expression is directed to the synapse/synaptic vesicle. The donating investigator reports that direct GFP fluorescence and direct tdTomato fluorescence may be visualized in these mice when tTA is present/dox is absent. In addition, the three Myc epi ..... For more information please see the full phenotype on the strain data sheet | ||
| 006514 | B6.Cg-Ighmbp2nmd-2J Tg(Ttn-Ighmbp2)108Cx/Cx | Research Strain |
| 006513 | B6.Cg-Ighmbp2nmd-2J Tg(Ttn-Ighmbp2)45Cx/Cx | Research Strain |
| 013115 | B6.Cg-Rag1tm1Mom Tg(UBC-GFP)30Scha/J | Research Strain |
| Tg(UBC-GFP)30Scha generates green fluorescent protein (GFP) expression in all cells of the body with cell lineage-specific variation in expression level. Hematopoetic cells have high expression levels compared with other cells and T cells have a 2-fold higher GFP expression level than that in CD19+B220+ B cells. The Rag1tm1Mom targeted disruption, which blocks the intragenic recombination essential for T and B cell antigen receptor formation, leaves homozygous mice immunocompromised due to the inability to form functional, mature T and B cells. This strain, which combines this targeted mutation with this transgene, is a universal host that does not reject engraftment regardless of histoincompatibility and permits detection of host-derived embryos or tissues by detection of GFP, as long as the donor tissue is not also engineered to express GFP. Animals from this strain can also be used as sentinel mice in specific pathogen free environments. ..... For more information please see the full phenotype on the strain data sheet | ||
| 003834 | B6.Cg-Tg(Eno2-Ighmbp2)90Cx Ighmbp2nmd-2J/Cx | Research Strain |
| Mice hemizygous for the transgene are viable and fertile. RT-PCR analysis indicates that transgene expression is limited to the central nervous system including forebrain, cerebellum and spinal cord. The presence of the transgene rescues the neuromuscular degeneration exhibited by nmd-2J mice. These mice have no obvious phenotype. This strain is useful for studies involving the role of Ighmpb2 in motor neuron disease. | ||
| 003833 | B6.Cg-Tg(Eno2-Ighmpb2)17Cx Ighmbp2nmd-2J/Cx | Research Strain |
| Mice hemizygous for the transgene are viable and fertile. RT-PCR analysis indicates that transgene expression is limited to the central nervous system including forebrain, cerebellum and spinal cord. The presence of the transgene rescues the neuromuscular degeneration exhibited by nmd-2J mice. These mice have no obvious phenotype. This strain is useful for studies involving the role of Ighmpb2 in motor neuron disease. | ||
| 012440 | DBA/2J-Tg(RP24-180N9)2Kjn/Kjn | Research Strain |
| The Tg(RP24-180N9)2Kjn transgene gives expression of Fscn2 and rescues the Fscn2ahl8 related early onset hearing loss on the DBA/2J background. At one month of age the 16 kHz ABR threshold of the hemizygote is close to that of DBA/2NCrl controls, a subline that does not have Fscn2ahl8. This transgenic rescue is similar to that found in the congenic rescue in D2.B6-Fscn2+/Kjn (stock #012438). | ||
| 004257 | NOD.Cg-Prkdcscid Tg(TcrLCMV)327Sdz/Dvs | Research Strain |
| These mice do not develop diabetes; The presence of the T cell receptor expressing transgene does not alter the resistance to type 1 diabetes conferred to the NOD background by homozygosity for the scid mutation. | ||
| 004334 | NOD/ShiLt-Tg(TcraAI4)1Dvs | Research Strain |
| 004335 | NOD/ShiLt-Tg(TcrbAI4)1Dvs | Research Strain |
| 005483 | 129-Tg(CAG-EYFP)7AC5Nagy/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for the transgenic insert (founder line 7AC5/EYFP) are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Homozygotes fluoresce with twice the intensity of the hemizygotes. Notable exceptions to this phenotype are erythrocytes and adipocytes in which fluorescence is negligible or absent. Please note, the original publication describing the creation and phenotype of mice harboring the pCX::EFYP transgene (Hadjantonakis 2002 BMC Biotechnol 2002 2:11) describes mice from founder line "YC5/EYFP" and indicates they are available through The Jackson Laboratory as Stock No. 003772. While the 003772 strain is no longer available, the donating investigators report that this Stock No. 005483 strain (from founder line "7AC5/EYFP") was generated from embryonic stem cell clones from the same experiment using the ..... | ||
| 005989 | 129;FVB-Tg(PTH-cre)4167Slib/J | Cryopreserved - Ready for recovery |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is observed only in parathyroid tissue; no activity is seen in thyroid, muscle, lymph node, trachea, thymus, salivary tissues, lung, heart, liver, brain, stomach, spleen, kidney, large intestine, small intestine, and pancreas. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in parathyroid-specific deletion of the flanked genome. These transgenic mice may be useful in generating mouse models of parathyroid-specific deletion of genes of interest, such as multiple endocrine neoplasia type 1, extracellular calcium-sensing receptor, and vitamin D receptor. | ||
| 006403 | 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this "K14-ER:Ras" transgene are viable and fertile, with expression of the ER:Ras fusion protein (constitutively active G12V mutant form of the catalytic domain of human H-ras (H-rasV12) fused at its amino terminal with the G525R mutant murine estrogen receptor ligand binding domain (ERTM)) directed to epidermis by the human keratin 14 promoter. Because of the ERTM region of the fusion protein, ER:Ras is restricted to the cytoplasm and the biochemical activity of the ER:Ras fusion gene can be induced following tamoxifen administration. For example, prolonged (4 weeks) induction of human H-RasG12V activity promotes the undifferentiated, proliferative phenotypic characteristics observed in epidermal cancer; including hyperplasia, increased mitotic index, decreased expression of differentiation markers and increased expression of beta-1 and beta-4 integrin subunits. H-RasG12V-induced skin abnormalities were entirely reversed ..... For more information please see the full phenotype on the strain data sheet | ||
| 006661 | 129S.B6-Tg(KRT14-RAF1/ESR1)1Pkha/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this "K14-Raf:ER" transgene are viable and fertile, with expression of the Raf:ER fusion protein (a constitutively active Y340D/Y341D mutant form of the catalytic domain of human Raf-1 (Raf-1[DD]) fused at its carboxy terminal with the G525R mutant human estrogen receptor ligand binding domain (ERTM)) directed to epidermis by the human keratin 14 promoter. Because of the ERTM region of the fusion protein, Raf:ER is restricted to the cytoplasm and the biochemical activity of the Raf:ER fusion gene can be induced following tamoxifen administration. For example, prolonged (4 weeks) induction of human Raf-1[DD] activity promotes the undifferentiated, proliferative phenotypic characteristics observed in epidermal cancer; including hyperplasia, increased mitotic index, decreased expression of differentiation markers and increased expression of beta-1 and beta-4 integrin subunits. Raf-1[DD]-induced skin abnormalities are entirely reversed within one m ..... For more information please see the full phenotype on the strain data sheet | ||
| 003960 | 129S6-Tg(Prnp-GFP/cre)1Blw/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Mice express the GFP/Cre fusion protein in widespread fashion. All tissues examined displayed Cre activity at an early (4-8 cell) embryonic stage. Germline expression is observed. Expression of GFP is less robust, being detectable in kidney tissue. | ||
| 008523 | 129S6.Cg-Tg(NPHS2-cre)295Lbh/BroJ | Cryopreserved - Ready for recovery |
| Mice harboring the p2.5P-Cre transgene are viable and fertile, with expression of Cre recombinase directed to podocytes within kidney glomeruli by the human podocin (NPHS2) promoter/enhancer region. Cre-recombinase activity is reported in podocytes during late capillary loop stage of glomerular development and persists in podocytes of mature glomeruli, with no evidence for cre expression detected in other tissues examined. Embryonic Cre-recombinase activity is also reported as early as 8.5 dpc. When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the sequence. These 2.5P-Cre transgenic mice may be useful in generating conditional knockouts for studying the role of podocyte nephrobiology in renal disorders. | ||
| 005308 | B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ | Cryopreserved - Ready for recovery |
| Transgenic mice are viable, fertile, normal in size, normoglycemic and do not display any gross physical or behavioral abnormalities.The TCR expressed from this transgene is specific for influenza virus A/PR/8 hemagglutinin (HA) in the context of the MHC class I molecule H2-Kd. Both thymic and peripheral T-cell populations are skewed toward CD8+ cells. The majority of thymocytes and virtually all CD8+ T cells in lymph nodes express the transgenic TCR beta chain. About 40% of peripheral blood CD8+ T cells react with the HA peptide presented by H2-Kd. When mated with Tg(Ins2-HA)165Bri, double transgenic neonates have similar levels of V-beta 8 and total number of thymocytes as Tg(TcraCl4,TcrbCl4) mice however the double transgenics become spontaneously diabetic after birth and die within 10 days. This transgenic model is useful in the study of T-cell activation, cross presentation of antigens, process of thymic selection, peripheral tolerance and ..... | ||
| 005534 | B10.Cg-H2d Tg(Ins2-HA)165Bri/ShrmJ | Cryopreserved - Ready for recovery |
| Transgenic mice are viable, fertile, normal in size, normoglycemic and do not display any gross physical or behavioral abnormalities. Mice homozygote for the transgene have silver grey fur color. Hemizygous and wildtype mice are black. Immunohistochemistry reveals pancreatic islet cell expression of the transgene and no expression in the spleen, kidney or thymus. Isolated islets stain normally for insulin and are morphologically indistinguishable from control islets. Additional functional studies found no expression in bone marrow. Histology revealed no insulitis and the single transgenic mice do not become diabetic. T-cell proliferation assays, Cytotoxic Lymphocyte (CTL) assays, and adoptive transfer studies performed using transgenic mice indicate significantly reduced class 1 and class II T-cell responses compared to controls. Hemagglutination inhibition assays of sera from HA primed transgenic mice indicate antibody titers slightly lower but nearly equivalent to HA primed control m ..... For more information please see the full phenotype on the strain data sheet | ||
| 006100 | B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J | Cryopreserved - Ready for recovery |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Mutant mice have the luciferase gene driven by two copies of the NF-kappaB (NF-kB or NFkB) regulatory element. The presence of nuclear NF-kB DNA binding activity (as detected by electrophoretic mobility shift assay [EMSA]) is consistent with luciferase reporter activity; these reporter mice identify NF-kB transcriptional activity in any tissue. These transgenic mice may be useful in studies of immunology, cellular signaling, signal transduction, apoptosis, and transcription factor function. | ||
| 006102 | B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J | Cryopreserved - Ready for recovery |
| Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator reports that homozygous females in their colony are subfertile. These transgenic mice express luciferase under the regulation of the Il2 minimal promoter and 3 binding sites for the NFAT inducible nuclear factor involved in the regulation of interleukin-2 and other cytokine expression. Luciferase activity in these transgenic mice identifies NFAT-mediated transcription. These NFAT-luc transgenic mice may be useful be useful as reporters for NFAT-mediated expression during thymocyte development and selection and in studies related to signal transduction, apoptosis, and transcriptional regulation. | ||
| 005895 | B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J | Cryopreserved - Ready for recovery |
| Male mice that are hemizygous for the Clone-1 TCR (also called Clone 1 Thy1.1 TCR or Cl.1 TCR) transgene are viable, fertile, and normal in size. Females are very weak and have low fecundity. The donating investigator reports that all transgenic mice are prone to tumor development by 5-6 months of age. The transgene encodes a rearranged low avidity T cell receptor that recognizes an influenza virus hemagglutinin epitope (HA518-526) restricted by MHC class I H-2Kd. Flow cytometric analysis shows appropriate skewing towards the CD8+ T cell compartment in thymocytes and peripheral lymphocytes. Both naive and activated clone 1 T cells exhibit decreased responsiveness when presented with their cognate antigen in vitro and when transferred into mice expressing HA on pancreatic beta cells. CD8+ T cells can be induced to exhibit both effector function and antitumor activity. This mouse is further modified with the Thy1.1 allele, rather than th ..... For more information please see the full phenotype on the strain data sheet | ||
| 003183 | B10.Cg-Tg(Igh2k3-83)1Nemz/J | Cryopreserved - Ready for recovery |
| Hemizygous mice carry the immunoglobulin transgene Tg(Igh2k3-83)1Nemz. Results indicate that B cells specific for major histocompatibility complex class I antigen can be deleted if they encounter membrane-bound antigen at a post-bone-marrow stage of development. This deletion may be necessary to prevent organ-specific autoimmunity. The 3-83 transgenic only produce B-lymphocytes that make a single type of antibody with a defined specificity whose presence or absence can be easily monitored with several different reagents. Thus, they are invaluable to investigators who wish to determine how the development of a specific antibody specificity is genetically controlled. This is an important question in determining both how immune responses are generated against infectious agents, while normally preventing the development of antibodies against self-proteins which can lead to serious autoimmune diseases such as lupus. The 3-83 mice can also be be used to dissect the antibody produc ..... For more information please see the full phenotype on the strain data sheet | ||
| 003147 | B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J | Cryopreserved - Ready for recovery |
| Clonal deletion of autoreactive T cells in vivo was studied using a peptide antigen to induce deletion of antigen-reactive thymocytes. Mice transgenic for a T cell receptor that reacts to this peptide (Tg(DO11.10)10Loh) contain thymocytes that progress from the immature to the mature phenotype. Intraperitoneal administration of the peptide antigen to transgenic mice results in a rapid deletion of the immature CD4+ CD8+ TCRlo thymocytes. Apoptosis of cortical thymocytes can be seen within 20 hours of treatment. These results provide direct evidence for the in vivo role of apoptosis in the development of antigen-induced tolerance. | ||
| 003199 | B10.PL-H2u H2-T18a/(73NS)Sn-Tg(TCRA)B1Jg/J | Cryopreserved - Ready for recovery |
| TCR-transgenic mice exhibit the generation of an unusual population of CD4-CD8-TCR+ thymocytes and the absence of gamma delta cells. Transgenic TCR alpha chain causes thymocytes to differentiate into a CD4-CD8-TCR+ lineage at an early developmental stage, depleting the number of thymocytes that differentiate into the alpha-beta lineage. This transgene is also associated with the development of T cell lymphosarcoma. In combination with the transgenic strain TCR beta, it can be used as a model for Multiple Sclerosis. | ||
| 003200 | B10.PL-H2u H2-T18a/(73NS)Sn-Tg(TCRB)C14Jg/J | Cryopreserved - Ready for recovery |
| TCR-transgenic mice exhibit the generation of an unusual population of CD4-CD8-TCR+ thymocytes and the absence of gama delta cells. Transgenic TCR beta chain causes immature T cells to accelerate differentiation into the alpha-beta lineage and thus inhibits the generation of gamma-delta cells. In combination with the transgenic strain TCR alpha, it can be used as a model for Multiple Sclerosis. | ||
| 009580 | B6(129S4)-Et(cre/ERT2)1382Rdav/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of the Cre-ERT2 fusion gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Cre recombinase activity is observed in brain tissues following tamoxifen administration (although some cre activity is reported prior to tamoxifen administration). The donating investigators may not have assessed expression in tissues other than brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
For characterization information, see images at the Allen Institute for Brain Science website (Et(cre/ERT2)1382Rdav images). The C ..... | ||
| 009583 | B6(129S4)-Et(cre/ERT2)1957Rdav/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of the Cre-ERT2 fusion gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Cre recombinase activity is observed in brain tissues following tamoxifen administration. The donating investigators may not have assessed expression in tissues other than brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
For characterization information, see images at the Allen Institute for Brain Science website (Et(cre/ERT2)1957Rdav images). The Cre-ERT2 fusion protein (Cre-ERT2) consists of Cre re ..... | ||
| 009584 | B6(129S4)-Et(cre/ERT2)2007Rdav/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of the Cre-ERT2 fusion gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Cre recombinase activity is observed in brain tissues following tamoxifen administration. The donating investigators may not have assessed expression in tissues other than brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
For characterization information, see images at the Allen Institute for Brain Science website (Et(cre/ERT2)2007Rdav images). The Cre-ERT2 fusion protein (Cre-ERT2) consists of Cre re ..... | ||
| 009574 | B6(129S4)-Et(cre/ERT2)21Rdav/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of the Cre-ERT2 fusion gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Cre recombinase activity is observed in brain tissues following tamoxifen administration. The donating investigators may not have assessed expression in tissues other than brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
For characterization information, see images at the Allen Institute for Brain Science website (Et(cre/ERT2)21Rdav images). The Cre-ERT2 fusion protein (Cre-ERT2) consists of Cre reco ..... | ||
| 009578 | B6(129S4)-Et(cre/ERT2)398Rdav/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of the Cre-ERT2 fusion gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Cre recombinase activity is observed in brain tissues following tamoxifen administration (although some cre activity is reported prior to tamoxifen administration). The donating investigators may not have assessed expression in tissues other than brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
For characterization information, see images at the Allen Institute for Brain Science website (Et(cre/ERT2)398Rdav images). The Cre-E ..... | ||
| 009573 | B6(129S4)-Et(cre/ERT2)4Rdav/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of the Cre-ERT2 fusion gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Cre recombinase activity is observed in brain tissues following tamoxifen administration. The donating investigators may not have assessed expression in tissues other than brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
For characterization information, see images at the Allen Institute for Brain Science website (Et(cre/ERT2)4Rdav images). The Cre-ERT2 fusion protein (Cre-ERT2) consists of Cre recomb ..... | ||
| 009579 | B6(129S4)-Et(cre/ERT2)837Rdav/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of the Cre-ERT2 fusion gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Cre recombinase activity is observed in brain tissues following tamoxifen administration. The donating investigators may not have assessed expression in tissues other than brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
For characterization information, see images at the Allen Institute for Brain Science website (Et(cre/ERT2)837Rdav images). The Cre-ERT2 fusion protein (Cre-ERT2) consists of Cre reco ..... | ||
| 009587 | B6(129S4)-Et(icre)1402Rdav/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of a codon-improved Cre recombinase (iCre) gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. The donating investigator reports Cre recombinase activity in brain tissues (but may not have assessed expression in other tissues). When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring. For characterization information, see images at the Allen Institute for Brain Science website (Et(icre)1402Rdav images). | ||
| 009588 | B6(129S4)-Et(icre)1470Rdav/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of a codon-improved Cre recombinase (iCre) gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. The donating investigator reports Cre recombinase activity in brain tissues (but may not have assessed expression in other tissues). When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring. For characterization information, see images at the Allen Institute for Brain Science website (Et(icre)1470Rdav images). | ||
| 009589 | B6(129S4)-Et(icre)1555Rdav/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of a codon-improved Cre recombinase (iCre) gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. The donating investigator reports Cre recombinase activity in brain tissues (but may not have assessed expression in other tissues). When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring. For characterization information, see images at the Allen Institute for Brain Science website (Et(icre)1555Rdav images). | ||
| 009586 | B6(129S4)-Et(icre)754Rdav/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of a codon-improved Cre recombinase (iCre) gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. The donating investigator reports Cre recombinase activity in brain tissues (but may not have assessed expression in other tissues). When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
For characterization information, see images at the Allen Institute for Brain Science website (Et(icre)754Rdav images). | ||
| 008617 | B6(A)-Tg(OPN1LW-DT)1Mame/J | Cryopreserved - Ready for recovery |
| Cone photoreceptors in these mice express a modified (DT176) diptheria toxin A chain (DTA) driven by the human red cone specific opsin promoter. This results in the ablation of the cone cells. These mice show no physical, behavioral, reproductive, or growth abnormalities, and the histologic appearance of their retinae remains unchanged over at least 8 months. This strain may be useful in studies of vision and signal processing within the central nervous system. | ||
| 003428 | B6(Cg)-Tg(B2M)1Trg Tg(HLA-B)1Trg/Dcr | Cryopreserved - Ready for recovery |
| 008619 | B6.129-Opn1mwtm1(OPN1LW)Nat/J | Cryopreserved - Ready for recovery |
| A portion of the X-linked mouse opsin 1 (medium-wave-sensitive) "green pigment" gene was replaced by a human opsin 1 (long-wave-sensitive) "red pigment" cDNA in these targeted mutant mice. Heterozyguous female mice are endowed with a unique ability to make chromatic discriminations at long wavelengths. Hemizygous males and homozygous females have greater sensitivity to long wavelength light than normal mice. Heterozygotes, hemizygotes, and homozygotes are normal in size, viability, and fertility. Expression is observed in retinal cone photoreceptors as determined by immunostaining. The knocked-in coding sequences are expressed normally. | ||
| 005301 | B6.129S2-Tg(APP)8.9Btla/J | Cryopreserved - Ready for recovery |
| These transgenic mice express all mRNA and protein isoforms of the wild-type human amyloid beta (A4) precursor protein, APP. The transgene expression level is equivalent to the level of endogenous mouse amyloid beta (A4) precursor protein (in the homozygous state). The expression pattern of the various protein isoforms of human APP mimics endogenous mouse gene expression patterns. These mice serve as a model for dosage imbalance for APP that occurs in Down syndrome and also provide a unique model to examine the regulation of APP isoforms, APP processing and amyloid beta metabolism and regulation. | ||
| 006469 | B6.129S4-Tg(PSEN1H163R)G9Btla/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this "H163R mutant PSEN1 YAC" transgene are viable and fertile, while the donating investigator reports that homozygous mice are non-viable. Semi-quantitative RT-PCR of multiple tissues shows expression of H163R mutant human PSEN1 at levels comparable to that of wildtype mouse PSEN1 (50–70%). In contrast to other PSEN1 transgenic models, tissues from this strain express alternatively spliced human PSEN1 transcripts encoding PSEN1 protein (with or without the tetrapeptide VRSQ) and accumulated an 18-kDa PSEN1 C-terminal fragment as shown by western blots, thus expressing a wide spectrum of different human PSEN1 mRNAs and proteins. When crossed to other FAD transgenic strains (for example Stock No. 005300), this transgene is associated with elevated levels of the 42 amino acid form of amyloid-beta (1–42) in both brain and plasma. These mice may be useful in studying neurological disorders such as Familial Alzheimer’s Disease and Down syndrome. | ||
| 005500 | B6.C-Tg(Ins2-GP)34-20Olds/MvhJ | Cryopreserved - Ready for recovery |
| Transgenic mice were created with the Lymphocytic choriomeningitis virus (LCMV) glycoprotein(GP) or nucleoprotein (NP) under the control of the rat insulin promoter. Ins2-GP expression was determined only in the pancreas by RT-PCR (von Herrath et al 1994). Tg(Ins2-GP)34-20Olds untreated mice rarely develop insulin-dependent diabetes mellitus (IDDM). When challenged with LCMV they develop IDDM. The B6.C -Tg(Ins2-GP)34-20Olds mice (H2b) exhibit a rapid (10-14 days) onset of IDDM compared to C.B6-Tg(Ins2-NP)25-3Olds mice (H2d) (10-21 days) or B6.C -Tg(Ins2-NP)25-3Olds mice (H2b) (30-120 days) (Oldstone et al.,1991; von Herrath et al.,1994). Thymic expression of nucleoprotein has been shown to be responsible for this delayed onset of IDDM. Thymi from newborn B6.C -Tg(Ins2-GP)34-20Olds transplanted into nude hosts produce a primary CTL response when challenged with LCMV. CD8 T cells are required for IDDM development in both glycoprotein ..... For more information please see the full phenotype on the strain data sheet | ||
| 003479 | B6.C3-Tg(Fos-luc)1Rnd/J | Cryopreserved - Ready for recovery |
| C57BL/6-TgN(c-fosLuc)1Rnd mice are viable and fertile. They carry a firefly luciferase reporter gene driven by the c-fos promoter. The luciferase transgene is expressed constitutively in all tissues of these mice, at the lowest levels in kidney, liver, lung, spleen, heart and various areas of the brain, and at the highest levels in skin and testis. Activation of the c-fos promoter in response to a wide range of stimuli results in increased luciferase expression. C57BL/6-TgN(c-fosLuc)1Rnd mice provide a unique system to monitor c-fos promoter activity in living tissue explant or dispersed cell cultures. | ||
| 004853 | B6.C3-Tg(KRT14-Birc5)19Gros/J | Cryopreserved - Ready for recovery |
| These transgenic mice express the mouse baculoviral IAP repeat-containing 5, Birc5 (survivin), gene under the direction of the human keratin 14 promoter. Transgene expression is specific to epidermal and follicular keratinocytes. Mice hemizygous for the transgenic insert are resistant to chemical (DMBA)- and UVB-induced keratinocyte apoptosis in vivo. Although hemizygotes were less susceptible to DMBA-induced papilloma formation, spontaneous papilloma regression was not observed and there was enhanced conversion o | ||