Search Criteria: Research Area is "Research Tools: Developmental Biology Research"
| Stock Number |
Strain Name Strain Description |
Standard Supply |
| 004337 | 129(Cg)-Foxg1tm1(cre)Skm/J | Repository- Live |
| This strain expresses Cre recombinase from the endogenous Foxg1 locus. Forkhead box G1 is required for telencephalon development and is expressed specifically in the telencephalon and discrete head structures. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in the telencephalon, anterior optic vesicle (developing lens and retina), otic vesicle, facial and head ectoderm, olfactory epithelium, mid-hindbrain junction and pharyngeal pouches. Mice that are homozygous for the targeted mutation die perinatally. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain represents a model that may be useful in studies of telencephalic development. | ||
| 008569 | 129-Alpltm1(cre)Nagy/J | Repository- Live |
| This strain expresses Cre recombinase from the targeted locus. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in tissue-specific deletion of the flanked sequence. Recombination occurs primarily in embryonic primordial germ cells. Approximately 60% of gonadal cells isolated from embryonic day 13.5 embryos exhibit Cre recombinase activity. Mosaic ectopic recombinase activity does occur. Homozygotes are not viable. This mutant mouse strain represents a model that may be useful in studies of reproductive and endocrine systems development. | ||
| 007915 | 129S.FVB-Tg(Amh-cre)8815Reb/J | Repository- Live |
| Mice harboring the Amh-cre transgene are viable and fertile, with expression of Cre recombinase directed by the testis Sertoli cell-specific promoter elements of the anti-Mullerian hormone (Amh) gene. Cre-recombinase activity is reported in testis Sertoli cells during male sexual development as early as E14.5, with no evidence for cre expression detected in other tissues examined. When Amh-cre transgenic males are bred with female mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence specifically in testis Sertoli cells. These Amh-cre transgenic mice may be useful in generating conditional knockouts in testis Sertoli cells for studying male embryonic sexual differentiation and the regulation of spermatogenesis. | ||
| 004218 | B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J | Repository- Live |
| Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Notable exceptions to this phenotype are erythrocytes and adipocytes in which fluorescence is negligible or absent. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available. | ||
| 008471 | B6.129(SJL)-Oxtrtm1.1Wsy/J | Repository- Live |
| Mice homozygous for the Oxtrflox allele are viable and fertile, with loxP sites flanking exons 2-3 of the targeted gene. Expression and receptor binding distributions from the Oxtrflox targeted allele are reported to be normal when compared to wild-type. When Oxtrflox mice are bred to mice that express Cre recombinase, the resulting offspring will have the floxed region deleted in the cre-expressing tissue(s). These Oxtrflox mice may be used for spatial and temporal inactivation of the oxytocin receptor in studying (for example) parturition and lactation, as well as social, behavioral, and learning disorders such as autism and anxiety.
These Oxtrflox mice may also be useful along with the Oxt/EGFP AI03 transgenic mice (Stock No. 006043) or oxytocin targeted mutant mice (Stock No. 002713) from the same ..... | ||
| 007741 | B6.129-Arg1tm1Rki/J | Repository- Live |
| Homozygous AI-mutant mice completely lack hepatic arginase (AI) activity, exhibit hyperagrininemia, severe symptoms of hyperammonemia (ncluding decerebrate posture, lethargy, and high-frequency tremor of the extremities, particularly the tail) and die between 10-14 days after birth. Neural stem cells (NCSs) isolated from homozygous mice exhibit abnormal proliferation and differentiation. In addition, haploid germ cells carrying the disrupted AI allele may be less fit/less effective in forming zygotes compared to wild-type spermatozoa. Heterozygotes are viable and fertile. These AI-mutant mice may be useful in studying metabolic defects of arginase I deficiency, urea cycle (excretion of excess nitrogen), and neuronal development and function. | ||
| 006910 | B6.129-Crkltm1Hkp/J | Repository- Live |
| While heterozygous mice are viable and fertile, mice homozygous for this targeted allele die in utero. Immunoblots from homozygous tissues show no protein expression from the targeted gene. The prenatal lethality exhibited by homozygotes on this C57BL/6J congenic background (and also on a 129Sv genetic background) likely results from heart, liver, and placental defects. Please note that homozygous mutants on a mixed/outbred genetic background (129/Sv X Black Swiss) are viable and fertile. These mutant mice may be useful in studying the role of Crkl tyrosine-phosphorylation in Bcr/Abl (Philadelphia chromosome) chronic myelogenous leukemia (CML), acute lymphoblastic leukemia (ALL), Digeorge Syndrome (DGS) and Velocardiofacial Syndrome. | ||
| 004152 | B6.129-Ctnnb1tm2Kem/KnwJ | Repository- Live |
| These mice possess loxP sites located in introns 1 and 6 of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to a strain expressing Cre recombinase in chrondocytes (see Stock No. 003554 for example), this mutant mouse strain may be useful in studies of chrondocyte differentiation. When bred to a strain expressing Cre recombinase in heart(see Stock No. 005650 or 005657 for example), this mutant mouse strain may be useful in studies of cardiovascular disease. When bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 007807 or > ..... | ||
| 008100 | B6.129-Prdm1tm1Clme/J | Repository- Live |
| These mice possess loxP sites in introns flanking exons 6 to 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 6 to 8 deleted in the cre-expressing tissue(s). When bred to a strain expressing Cre recombinase during B-lymphocyte development and differentiation (see Stock No. 004126 for example), this mutant mouse strain may be useful in studies of humoral immune response. | ||
| 011029 | B6.129-Rpl22tm1.1Psam/J | Repository- Live |
| Mice homozygous for this RiboTag allele are viable and fertile, with a targeted mutation of the ribosomal protein L22 (Rpl22) locus harboring a loxP-flanked wildtype C-terminal exon 4 followed by an identical C-terminal exon 4 that is tagged with three copies of the hemagglutinin (HA) epitope before the stop codon. Prior to exposure to Cre recombinase, RiboTag mice express the wildtype RPL22 protein (15 kDa). When the RiboTag mice are bred to cre-expressing mice, offspring will have the floxed wildtype exon 4 deleted in the cre-expressing tissue and subsequent use of the downstream HA epitope-tagged exon 4 as the terminal exon. The 23 kDa HA epitope-tagged ribosomal protein (RPL22HA) is incorporated into actively translating polyribosomes. These RiboTag mice allow Cre-mediated HA epitope tagging of ribosomes from user-defined cell types and/or immunoprecipitation of ribosomes bound to mRNA from those specific cell types. | ||
| 008451 | B6.129P(Cg)-Ptprca Cx3cr1tm1Litt/LittJ | Repository- Live |
| Mice that are homozygous for the CX3CR1-GFP targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. RT-PCR analysis of lymphoid tissue from homozygotes detects mutant gene product (mRNA) and no wild type gene product (mRNA). Flow cytometric analysis of peripheral blood cells identified a subset of green fluorescent cells not observed in wild type mice. Enhanced Green Fluorescent Protein (EGFP), but not the endogenous gene, is expressed in monocytes, dendritic cells, NK cells, and brain microglia, mimicking endogenous gene expression. The same subset of peripheral blood cells isolated from heterozygote mice express detectable levels of EGFP. Immunohistochemical analysis of spleen and peripheral nerve tissue from homozygotes does not detect EGFP. These mice also express the CD45.1 (Ly5.1 or Ptprca) allele, which is atypical for the C57BL/6 congenic background, and this marker may be used to track donor cell popul ..... For more information please see the full phenotype on the strain data sheet | ||
| 005582 | B6.129P-Cx3cr1tm1Litt/J | Repository- Live |
| Mice that are homozygous for the CX3CR1-GFP targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. RT-PCR analysis of lymphoid tissue from homozygotes detects mutant gene product (mRNA) and no wild type gene product (mRNA). Flow cytometric analysis of peripheral blood cells identified a subset of green fluorescent cells not observed in wild type mice. Enhanced Green Fluorescent Protein (EGFP), but not the endogenous gene, is expressed in monocytes, dendritic cells, NK cells, and brain microglia, mimicking endogenous gene expression. The same subset of peripheral blood cells isolated from heterozygote mice express detectable levels of EGFP. Immunohistochemical analysis of spleen and peripheral nerve tissue from homozygotes does not detect EGFP. These CX3CR1-GFP mutant mice may be useful in studies of leukocyte migration and trafficking, as well as for transplantation studies.
Of note, CX3CR1-GFP mice are also avail ..... | ||
| 006785 | B6.129P2(C)-Cd19tm1(cre)Cgn/J | Repository- Live |
| Homozygous mutant mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Homozygotes have a deficiency in the B-1 subset of B-lymphocytes along with a concomitant reduction in serum IgM. Their ability to respond to T-cell-dependent antigens is severely impaired, and they fail to form splenic germinal centers. In addition to disrupting the targeted gene, the targeting construct also introduced a cre cassette into exon 2 of the targeted gene, effectively placing cre expression under the control of the endogenous promoter. The Cd19 promoter specifically directs cre expression at the earliest stages and throughout B-lymphocyte development and differentiation. Although homozygous mutant mice are Cd19-deficient, heterozygous mice are phenotypically normal, and can be used for specific deletion of loxP-flanked (floxed) targets in B-lymphocytes.
In an attempt to offer alleles on well-characte ..... | ||
| 006084 | B6.129P2(Cg)-Foxg1tm1(cre)Skm/J | Repository- Live |
| This strain expresses Cre recombinase from the endogenous Foxg1 locus. Forkhead box G1 is required for telencephalon development and is expressed specifically in the telencephalon and discrete head structures. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in the telencephalon, anterior optic vesicle (developing lens and retina), otic vesicle, facial and head ectoderm, olfactory epithelium, mid-hindbrain junction and pharyngeal pouches. Mice that are homozygous for the targeted mutation die perinatally. Heterozygous mutant mice are viable, fertile, normal in size. On the C57BL/6 background, forebrain volume in heterozygotes is substantially reduced especially in the cerebral cortex (40.7%), striatum (29.7%), and hippocampus (18.6%). In the adult, the thalamus is reduced in volume by 21.6%. This mutant mouse strain represents a model that ma ..... For more information please see the full phenotype on the strain data sheet | ||
| 011008 | B6.129P2(Cg)-Gt(ROSA)26Sortm1(tTA)Roos/J | Repository- Live |
| Mice heterozygous for the ROSA:LNL:tTA conditional mutation of the Gt(ROSA)26Sor locus are viable and fertile, with a loxP-flanked STOP cassette preventing transcription of a downstream optimized/modified tetracycline-controlled transactivator protein ("mtTA"). Applying both Cre-lox and Tet-Off technologies, these ROSA:LNL:tTA mutant mice may be useful to generate compound mutant mice in which expression of a tetracycline-responsive promoter element (TRE; also called tet-operator or tetO)-driven gene of interest can be both directed to the cell types defined by the chosen Cre recombinase expression, as well as turned off by the addition of tetracycline (or its analog doxycycline (dox)). | ||
| 007572 | B6.129P2(Cg)-Rorctm2Litt/J | Repository- Live |
| Mice homozygous for this Rorc(γtGFP (or RORγt)GFP) mutant allele are viable and fertile. While Rorcγ mRNA is detected in liver in Rorc(γ)tGFP homozygotes, mRNA and protein for the thymus-specific isoform (Rorcγt) encoded by the targeted allele are not detected in the thymus. EGFP expression reports Rorc(γt) transcription in the thymi of adult Rorc(γt)GFP mice. Homozygous mice exhibit abnormal lymph node, Peyer's patch, and lymphoid tissue inducer (LTi) cell development. Mice with Rorcγt-deficient T cells lack tissue-infiltrating proinflammatory T-helper cells (Th17 cells), and are protected from induced autoimmune disease (EAE) on this genetic background. The donating investigator also reports increased thymoma incidence with age in homozygotes. These RorcγtGFP mutant mice may be useful in studying immune system homeostasis, T cell repertoire selection, CD4/CD8 double positive (CD4+/CD8 For more information please see the full phenotype on the strain data sheet | ||
| 008875 | B6.129P2-Lgr5tm1(cre/ERT2)Cle/J | Repository- Live |
| While homozygous mice are not viable, heterozygous Lgr5-EGFP-IRES-CreERT2 mice are viable and fertile; harboring a Lgr5-EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 (Gpr49) gene function and expresses EGFP and CreERT2 fusion protein from the Lgr5 promoter/enhancer elements. EGFP fluorescence is observed in crypt base columnar cells in small intestine (aka stem cells of the small intestine) and colon. Cre-ERT2 fusion gene activity is inducible; observed in the same cells only following tamoxifen administration. EGFP or inducible CreERT2 expression may also be observed in other Lgr5-expressing cell types (including pre-malignant mouse adenomas, colon cancer cells, epithelial stem cells of the stomach gland, basal epithelial layer stem cells of the mammary glands, and hair follicle stem cells).
The donating investigator reports variegated expression of the Lgr5-EGFP-IRES-CreERT2 transgene in the small intestine and colon (something which may h ..... | ||
| 007177 | B6.129P2-Mecp2tm1Bird/J | Repository- Live |
| These mice possess two functional loxP sites flanking exons 3-4 of the targeted gene on the X chromosome (the donating investigator reports that the middle loxP site is non-functional). Homozygous females and hemizygous males are viable and fertile. Northern blot analysis showed the expected mature transcript from the Mecp2lox locus as well as a transcript in which the beta-globin intron was unspliced. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3-4 deleted in the cre-expressing tissue(s). Mice with this X-linked floxed mutation may be useful in neurological and developmental studies of Rett syndrome. For example, when crossed to a strain expressing Cre recombinase in nervous tissue (see Stock No. 003771), this mutant mouse strain develops a neurological phenotype that mimics Rett syndrome. When crossed to a strain expressing Cre ..... | ||
| 006849 | B6.129P2-Mecp2tm2Bird/J | Repository- Live |
| These mice possess a loxP-flanked STOP cassette in intron 2 of the targeted gene on the X chromosome. Western blot and hybridization analysis confirm the absence of wildtype protein from the targeted allele (although the donating investigator reports that the targeted allele produces a "read-through" transcript which does not give rise to detectable levels of protein but makes it difficult to discriminate between the "flox-stopped" and reactivated alleles by RT-PCR). Hemizygous (Mecp2lox-Stop/y) males do not breed and develop Rett syndrome symptoms (reduced mobility, hindlimb clasping) at approximately 6 weeks of age, with death occurring at approximately 11 weeks of age. Heterozygous females are fertile until developing Rett syndrome characteristics at 4-12 months of age. This Rett syndrome-like phenotype is similar to that observed for the traditional knock-out allele (see Stock No. 003890). Cre recombin ..... For more information please see the full phenotype on the strain data sheet | ||
| 008336 | B6.129P2-Ptpn6tm1Rsky/J | Repository- Live |
| Mice homozygous for the Ptpn6f allele are viable and fertile, with loxP sites flanking exon 1(II) through most of exon 9 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in cre-expressing tissue(s). These Ptpn6f mice may be useful in generating conditional mutations for studying the role of Ptpn6 (Shp1) in inflammation and immunology research. For example, when bred to a strain with inducible Cre recombinase expression in liver and lymphocytes (see Stock No. 003556), this mutant mouse strain may be useful in studying the motheaten (me) phenotype; characterized by widespread inflammation and autoimmunity.
When bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 004126, Stock No. > ..... | ||
| 016222 | B6.129S(Cg)-Id2tm1.1(cre/ERT2)Blh/ZhuJ | Repository- Live |
| The Id2-CreERT2 knockin allele was designed to both abolish inhibitor of DNA binding 2 (Id2) gene function and express CreERT2 fusion protein from the Id2 promoter/enhancer elements. Cre-ERT2 fusion gene activity is inducible and can be observed following tamoxifen administration. As such, when Id2-CreERT2 knockin mice are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in the Id2-expressing cells of the offspring.
No mRNA or protein expression from the Id2-CreERT2 allele is observed. The donating investigator reports that homozygous mice are runted with defective lung alveolarization. Other organ systems have not been evaluated. However, Id2-CreERT2 homozygotes may be expected to exhibit the same phenotype as mice homozygous for other null mutations of this gene (including postnatal lethality and defects of the immune system, digestive tract, ..... | ||
| 013594 | B6.129S-Atoh1tm5.1(Cre/PGR)Hzo/J | Repository- Live |
| A targeting vector was designed to replace the coding sequence of the atonal homolog 1 (Atoh1) gene with a modified Cre-recombinase-progesterone receptor fusion protein (Cre-PR), abolishing gene function. Homozygous Math1Cre*PR mice die before birth due to central respiratory failure. Heterozygous mice are viable,fertile, and normal in size. The Math1Cre*PR/+ allele has expression of the Cre*PR fusion protein under control of the Math1 promoter/enhancer elements. Cre*PR fusion gene activity is inducible; observed only 6-12 hours after administration of RU486, a competitive progesterone receptor antagonist. As such, when Math1Cre*PR/+ mice are bred with mice containing loxP-flanked sequence, RU486-inducible Cre-mediated recombination will result in deletion of the floxed sequences in the Math1-expressing cells of the offspring. As such Math1 is expressed in the hindbrain, specifically in the conscious pro ..... For more information please see the full phenotype on the strain data sheet | ||
| 013595 | B6.129S-Atoh1tm6.1Hzo/J | Repository- Live |
| These Atoh1FLAG mice contain three c-terminal FLAG tags fused to the 3' end of the atonal homolog 1 (Atoh1) gene. Homozygous mice are viable and fertile. Atoh1FLAG expression is evident evident in all Atoh1 expressing cells including cerebellar granule neuron precursors (GNPs) where it binds the active transcriptional enhancer region of the GLI-Kruppel family member GLI2 (Gli2) gene. These mice may be useful for studying postnatal cerebellar development and regulation of Sonic Hedgehog-induced medulloblastoma. | ||
| 010525 | B6.129S-Notch2tm3Grid/J | Repository- Live |
| These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s).
When bred to a strain with early embryonic Cre recombinase expression (see Stock No. 003755 for example), this mutant mouse strain may be useful in studies of the Notch pathway during development. When bred to a strain expressing Cre recombinase in embryos, in particular, cardiac neural crest cells (see Stock No. 005549 for example), this mutant mouse strain may be useful in studies of vascular smooth muscle development and the cardiovascular defects associated with Alagille syndrome ..... | ||
| 009125 | B6.129S1(Cg)-Lmnatm1Stw/BkknJ | Repository- Live |
| Mice heterozygotes for this lamin A/C mutation are viable and fertile. The targeted allele does not express both full-length transcripts or stable lamin A/C protein. Homozygotes (Lmna -/- mice) exhibit severely retarded postnatal growth beginning as early as 2 weeks of age and abnormal movement/gait by 3-4 weeks of age that progresses to distinct scoliosis/kyphosis and death around 8 weeks of age. Lmna -/- mice also have tissue-specific alterations of nuclear envelope integrity and mislocalization of the inner nuclear membrane protein emerin. In skeletal and cardiac muscle, this results in rapid myopathic onset closely resembling Emery-Dreifuss muscular dystrophy (EDMD). These mice are a model for the autosomal variant of EDMD and may be useful in studying the role of lamins, inner nuclear membrane proteins, nuclear envelope integrity, and chromatin domain anchoring sites in EDMD.
In an attempt to offer alleles on well-characterized or multiple genetic background ..... | ||
| 009089 | B6.129S1(Cg)-Ndntm2Stw/J | Repository- Live |
| The mouse locus 7qB4/B5 (syntenic with the Prader-Willi region at chromosome position 15q11-q13 in humans) encompasses the cluster of paternally-expressed imprinted genes Magel2, Ndn, Mkrn3, and Peg12. As maternal imprinting silences the Ndn allele, only the paternally inherited Ndn allele is expressed. The Ndntm2Stw (ΔNdn-lacZ or Ndn-lacZ) knock-in allele abolishes endogenous gene function and expresses a β-galactosidase fusion protein. Under control of the upstream promoter/enhancer elements, lacZ expression is directed to the same tissues as the wildtype gene. For example, β-galactosidase expression during embryogenesis is highest in hypothalamus, but also detected in other central nervous system tissues (pons and medulla, spinal cord), peripheral nervous system (dorsal root ganglia), and some non-neuronal tissues (tongue, cartilage brown fat). Breeding heterozygous females with wildtype m ..... For more information please see the full phenotype on the strain data sheet | ||
| 006600 | B6.129S1-Mnx1tm4(cre)Tmj/J | Repository- Live |
| Mice heterozygous for this HB9cre targeted mutation are viable and fertile, with cre expression replacing HB9 (Hlxb9 or Mnx1) expression. Under control of the endogenous upstream elements, cre expression is directed to motor neurons. In heterozygotes, cre expression coincides with HB9 expression. Homozygous HB9cre mice die at or soon after birth, with expression of Cre recombinase likewise directed to motor neurons but no expression of endogenous HB9. When these HB9cre mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination in the resulting offspring leads to deletion of the flanked sequences in Mnx1/HB9 expressing cells; making them useful in neurodevelopmental studies of homeobox genes, motor neuron function and differentiation, and the central nervous system. | ||
| 009387 | B6.129S1-Osr1tm1Jian/J | Repository- Live |
| The Osr1tm1Jian (Odd1-LacZ) mutation abolishes endogenous gene function and expresses a β-galactosidase fusion protein (fused in-frame with the N-terminal 16 amino acid residues of the endogenous protein). Under control of the upstream promoter/enhancer elements, lacZ expression is directed to the same tissues as the wild-type gene (for example, β-galactosidase expression during embryogenesis is detected at E7.5 in the intermediate mesoderm, is expanded to the gut endoderm, lung bud mesenchyme and myocardial cells by E9.5, and is activated in developing branchial arches and limb buds by E10.5). Almost all (`95%) of homozygous mice die in utero between E11.5-E12.5 from circulation distress; exhibiting malformed atrial septum, dilated atria with hypoplastic venous valves, and blood backflow from the heart into systemic veins. Homozygotes also exhibit complete agenesis of adrenal glands, metanephric kidneys, gonads, and defects in pericard ..... For more information please see the full phenotype on the strain data sheet | ||
| 009386 | B6.129S1-Osr2tm1Jian/J | Repository- Live |
| The Osr2-lacZ mutation abolishes endogenous gene function and expresses a β-galactosidase fusion protein (fused in-frame with the N-terminal 15 amino acid residues of the endogenous protein). Under control of the upstream promoter/enhancer elements, lacZ expression is directed to the same tissues as the wild-type gene (for example, β-galactosidase expression during embryogenesis is detected by E9.5 in the mesonephric vesicles). Homozygous mice die shortly after birth with open eyelids, bilateral cleft of the secondary palate, and thickened tympanic rings. Heterozygotes are viable and fertile. These Osr2-lacZ mice may be useful as a lacZ reporter for Osr2 expression or as a knockout model for studying developmental biology (craniofacial, limb, and kidney). | ||
| 010617 | B6.129S1-Snai2tm1Grid/J | Repository- Live |
| Mice that are homozygous for the targeted mutation are viable, subfertile, and smaller in size compared to wildtype controls. These mice express a beta galactosidase fusion protein with 120 amino acids from the first half of the endogenous protein. The beta galactosidase staining pattern mimics the endogenous gene expression pattern in the embryonic limb buds and extraembryonic tissue. Homozygous adults develop eye infections (suppurative conjunctivitis) due to swollen eyelids. When challenged with UV radiation exposure, homozygotes exhibit decreased inflammation, lower skin tumor burden and fewer spindle cell tumors compared to wildtype. | ||
| 005628 | B6.129S2-Emx1tm1(cre)Krj/J | Repository- Live |
| Mice homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain expresses Cre recombinase from the endogenous Emx1 locus. Western blot analysis of cortical brain tissue does not detect reduced endogenous gene product (protein). When crossed with a strain containing a loxP-site flanked sequence, Cre-mediated recombination results in tissue-specific deletion of the flanked sequence. Recombination occurs in approximately 88% of the neurons of the neocortex and hippocampus, and in the glial cells of the pallium, mimicking the pattern of expression of the endogenous gene. Further, the donating investigator reports that Cre recombinase is also expressed in a subset of male germline cells, thus some offspring from a cre; floxed male will have the floxed allele recombined in all cells. This mutant mouse strain represents a model that may be useful in studies of forebrain development and f ..... For more information please see the full phenotype on the strain data sheet | ||
| 014089 | B6.129S2-Rbfox1tm1.1Dblk/J | Repository- Live |
| These Fox1flox mutant mice possess loxP sites flanking exons 11-12 of the RNA binding protein, fox-1 homolog 1 (Rbfox1) gene. Mice that are homozygous for this allele are viable, fertile, and normal in size. Fox-1 is expressed in brain, heart, and skeletal muscle and regulates alternative splicing in vertebrates by binding specifically to (U)GCAUG sequences. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exons 11-12 deleted in the Cre-expressing tissue, resulting in inactivation of Fox-1 gene function.
For example, when crossed to a strain expressing Cre recombinase in the central and peripheral nervous system (see Stock No. 003771), this mutant mouse strain may be useful in studies of neuronal excitation and seizures. | ||
| 007668 | B6.129S4(Cg)-Arntltm1Weit/J | Repository- Live |
| Mice homozygous for this conditional (floxed) allele possess loxP sites flanking exon 8 of the targeted gene and are viable and fertile, with circadian behavioral rhythms indistinguishable from wildtype littermates. When bred to mice that express Cre recombinase, the resulting offspring will have the exon encoding the ARNTL (BMAL1) basic helix-loop-helix (bHLH) domain deleted in the cre-expressing tissue(s). These Bmal1-floxed mutant mice may be useful in generating conditional mutations (whole-mouse or tissue-specific) to study the role of circadian clock/circadian rhythm in physiological and behavioral regulation.
For example, when crossed to a strain expressing Cre recombinase in the retina (see Stock No. 005105), this mutant mouse strain may be useful in studies of the circadian clock of the retina. | ||
| 014156 | B6.129S4(Cg)-Cdk5tm1.1Lht/J | Repository- Live |
| These Cdk5 floxed mutant mice possess loxP sites flanking exons 1-5. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, resulting offspring lack detectable Cdk5 in the cre-expressing tissues. | ||
| 003755 | B6.129S4-Meox2tm1(cre)Sor/J | Repository- Live |
| This strain expresses Cre recombinase under the control of the endogenous Meox2 promoter. Expression of Cre recombinase is observed in epiblast-derived tissues as early as embryonic day 5. The insertion creates a null allele for the Meox2 gene. Homozygous mice are viable on this background but exhibit an overall reduction in muscle mass and the absence of specific muscles resulting in abnormal limb posture and reduced motility. This phenotype is variable. As many as 80% of homozygotes are severely affected, fail to thrive and die before weaning. Some homozygotes (10%) exhibit clefting of the secondary palate. These mice can be utilized as a deleter strain for loxP flanked DNA and provide an alternative to tetraploid embryo analysis. | ||
| 011009 | B6.129S4-Mtortm1.2Koz/J | Repository- Live |
| Mice homozygous for this mTORfl allele are viable and fertile, with loxP sites flanking exons 1-5 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the putative transcription start site and the coding sequence through coding exon 5 deleted in the cre-expressing tissue(s). These mTORfl mutant mice may be useful in generating conditional mutations for studying mTOR and mTOR complex signaling; including regulation of cell growth, cell proliferation/differentiation, cell survival, cancer, tumor cell motility and metastasis, the RTK-PI3K-mTOR signaling axis, stem cell development, cytokine signaling, and T cell lineage commitment.
For example, when bred to a strain expressing Cre recombinase in adult striated muscle fibers and embryonic striated muscle cells of the somites and heart (see Stock No. 006149 for example), ..... | ||
| 007893 | B6.129S4-Myf5tm3(cre)Sor/J | Repository- Live |
| This strain expresses Cre recombinase from the endogenous Myf5 locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs skeletal muscle and the dermis, and in several ectopic locations. Homozygotes for this allele have a perinatal lethal phenotype and die at birth. Homozygotes display abnormal rib development and some fusions of the cervical or thoracic vertebrae. This mutant mouse strain represents a model that may be useful in studies of skeletal development.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as p ..... | ||
| 007669 | B6.129S4-Pdgfratm11(EGFP)Sor/J | Repository- Live |
| Mice homozygous for this knock-in targeted mutation have an embryonic lethal phenotype, with half of the embryos failing to survive past embryonic day 12.5 and the remainder failing to survive beyond embryonic day 15.5. These mice express the H2B-eGFP fusion gene from the endogenous Pdgfra locus. Fluorescence is detectable at embryonic day 4.5 in polar trophectoderm cells and at embryonic day 6.5 in the extraembryonic ectoderm. Expression of H2BGFP mimick the expression pattern of the endogenous gene. Homozygotes exhibit abnormal placenta development and placenta vasculature. This mutant mouse strain may be useful in studies of cellular signaling during development and in adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family). | ||
| 006440 | B6.129S4-Ptentm1Hwu/J | Repository- Live |
| These mice possess loxP sites flanking exon 5 of the targeted gene. Mice homozygous for the"floxed" allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.
For example, when crossed to a strain expressing Cre recombinase in astrocytes (see Stock No. 012887), this mutant mouse strain may be useful in studies of neurogenesis. When crossed to a strain expressing Cre recombinase in the central nervous system (see Stock No. 004600), this mutant mouse strain may be useful in studies of glia differentiation and cerebellar development. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to ..... | ||
| 007611 | B6.129S6(SJL)-Cdh2tm1Glr/J | Repository- Live |
| These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissue(s). When bred to a strain with inducible Cre recombinase expression in cardiac cells (see Stock No. 005657 for example), this mutant mouse strain may be useful in studies of myocardium physiology. | ||
| 015840 | B6.129S6-Itga8tm1.1Rdav/J | Repository- Live |
| Mice homozygous for this f(α8) conditional allele are viable and fertile, with loxP sites flanking the last two coding exons (exons 29-30) of the Itga8 gene (also called integrin alpha 8, alpha8-integrin, or α8-integrin). When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the transmembrane and the cytoplasmic domains deleted in the cre-expressing tissue(s). These f(α8) mutant mice may be useful in generating conditional mutations for studying the role of Itga8 transmembrane cell adhesion receptors in neuronal function in the developing and adult central nervous system, including intracellular signaling, behavior, synaptic plasticity, and memory formation.
For example, when f(α8) mice are bred to mice expressing Cre recombinase in forebrain neurons (see Stock No. 005359 for example), the double mutant offspring may exhibit impa ..... | ||
| 005623 | B6.129S6-Shhtm2(cre/ERT2)Cjt/J | Repository- Live |
| This strain expresses a fusion product involving Cre recombinase and a mutant form of the human estrogen receptor ligand binding domain from the endogenous Shh locus. The mutant human estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/ESR1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. Tamoxifen administration induces Cre recombinase expression in all cells that express the endogenous gene resulting in the deletion of the first 35 base pairs following the ATG. Homozygous mice are not viable or fertile. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of limb patterning and development. | ||
| 006878 | B6.129S6-Taglntm2(cre)Yec/J | Repository- Live |
| Mice homozygous for this SM22alpha-CreKI allele are viable and fertile. These mice have a Cre-recombinase gene inserted into the endogenous transgelin (SM22alpha) locus. The donating investigator reports that this mutation results in a loss of function of the targeted gene. Cre recombinase activity is shown in adult smooth muscle cells (such as arteries, veins, and visceral organs) and cardiac myocytes, but activity is not observed in the same embryonic tissues. It has been the experience of The Jackson Laboratory that optimal breeding is achieved by mating heterozygous females to homozygous males as female mortality post gestation has been noted in our colony. These SM22alpha-CreKI mice may be useful for Cre-lox technology applications in studying smooth muscle and cardiac gene function, as well as cardiovascular disease. | ||
| 012565 | B6.129S7(129S4)-Ift20tm1.1Gjp/J | Repository- Live |
| These Ift20flox mice harbor loxP sites flanking exons 2-3 (encoding the first 71 codons including the start codon) of the intraflagellar transport 20 homolog (Chlamydomonas) locus. The primary cilium is a microtubule-based antenna-like structure that emanates from the surface of virtually all cells in the mammalian body. The primary cilium functions as a sensory organelle (mechano-, chemo-, photo-receptor) that receives signals from other cells/the environment, and transmits these signals to the nucleus to elicit a cellular response. Most types of eukaryotic cilia and flagella are assembled and maintained by the process of intraflagellar transport (IFT). During IFT, large protein complexes (IFT particles) are transported along the ciliary microtubules under the ciliary membrane. IFT particle proteins organize into at least three distinct complexes called complex A, complex B and the Golgi IFT complex. The unique role of Ift20 in both complex B as well as the Golgi IFT ..... For more information please see the full phenotype on the strain data sheet | ||
| 008039 | B6.129S7-Gja1tm1Dlg/J | Repository- Live |
| Mice homozygous for this Cx43flox conditional allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. Presence of the loxP sites has no reported affect on expression of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have exon 2 deleted in the cre-expressing tissue(s). These Cx43flox mutant mice may be useful in generating conditional mutations for studying the role of connexin and gap junctions in various tissues and systems, including the cardiovascular system. | ||
| 002192 | B6.129S7-Gt(ROSA)26Sor/J | Repository- Live |
| Mice heterozygous or homozygous for the ROSA26 retroviral insertion display no distinguishing phenotype. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult mouse.
If breeding heterozygous mice together, recovery of homozygous offspring is significantly reduced. For unknown reasons, homozygous mutant mice are prone to convulsions. If breeding or creating homozygous mice, they should be handled carefully and quietly to avoid poor breeding, loss of litters, or premature death. | ||
| 008818 | B6.129S7-Itga3tm1Rdav/J | Repository- Live |
| Mice homozygous for this f(α3) conditional allele are viable and fertile, with loxP sites flanking exons 11-18 of the Itga3 (integrin alpha 3 (or alpha3-integrin (α3-integrin)) gene. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed region deleted in the cre-expressing tissue(s): such deletion leads to a non-sense mutation from direct splicing of exon 10 to exon 19 and results in a truncated peptide that is predicted to be missing more than half of the wild-type sequence, including those that encode the transmembrane and the cytoplasmic domains. These f(α3) mutant mice may be useful in generating conditional mutations for studying the role of Itga3 transmembrane cell adhesion receptors in neuronal functions in the developing and adult central nervous system, including synaptic plasticity and memory formation.
When bred to a strain expressing Cre recombinase in the hippocampal CA1 pyramidal cells ..... | ||
| 007579 | B6.129X1(Cg)-Fgfr2tm1Dor/J | Repository- Live |
| Mice homozygous for this Fgfr2flox allele possess loxP sites flanking exons 8-10 of the targeted gene and are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have sequences encoding the alternatively spliced Ig domain IIIb, as well as the IIIc and TM domains, deleted in the cre-expressing tissue(s). These Fgfr2-flox mutant mice may be useful in generating conditional mutations to study the role of fibroblast growth factor receptors in vertebrate development; including early embryogenesis, regional specification of the brain, limb morphogenesis, and normal bone, craniofacial, and lens development.
For example, when crossed to a strain expressing Cre recombinase in the central nervous system, especially astrocytes (see Stock No. 004600), this mutant mouse strain may be useful in studies of astroglial migration. When crossed to ..... | ||
| 006148 | B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J | Repository- Live |
| Mice that are homozygous for the R26-stop-EYFP mutant allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice contain an Enhanced Yellow Fluorescent Protein gene (EYFP) inserted into the Gt(ROSA)26Sor locus. Expression of EYFP is blocked by an upstream loxP-flanked STOP sequence. When bred to mice with a cre recombinase gene under the control of a promoter of interest, the STOP sequence of the targeted gene is deleted in the tissue of interest, and EYFP expression is observed. These mutant mice may be useful in monitoring the Cre expression in living tissues, and tracing the lineage of such cells in embryos, young, and adult mice at desired time points.
These R26-stop-EYFP have also been used as a fluorescent reporter by the Allen Institute for Brain Science. For their characterization information, see images at the Allen Institute for Brain Science website (> ..... | ||
| 007181 | B6.129X1-Notch1tm2Rko/GridJ | Repository- Live |
| Mice homozygous for this "floxed" Notch1 allele (fN1) are viable and fertile. These mice possess loxP sites on either side of exon 1 of the targeted gene. When bred to mice with a Cre recombinase gene, exon 1 of the targeted gene is deleted in the cre expressing tissue(s). These conditional knockout mice may be useful in generating tissue-specific mutants for studying the development of a wide range of tissues: for example, when crossed to a strain expressing Cre recombinase primarily in the nervous system (see Stock No. 003771), this mutant strain may be useful in studies of apoptosis in neural development.
When crossed to a strain expressing a differential Cre mediated reporter protein labeling: Notch1 signaling in actively cycling stem/progenitor cells (see Stock No. 006953), this mutant strain may be useful in studies of loss of Notch1 heterozyg ..... | ||
| 008712 | B6.129X1-Twist2tm1.1(cre)Dor/J | Repository- Live |
| Dermo1-cre (Twist2-cre) mutant mice harbor a Cre recombinase "knock-in" allele that also abolishes endogenous Twist2 gene function. Heterozygotes are viable and fertile, while homozygotes (twist-2-/-) die a few days after birth. Under control of the upstream promoter/enhancer elements, cre expression is observed in a pattern consistent with the wildtype gene; Cre recombinase activity is reported in mesoderm as early as embryonic day 9.5, in mesodermal tissues such as branchial arches and somites, and in condensed mesenchyme-derived chondrocytes and osteoblasts. When heterozygotes are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequences in Dermo1-expressing tissues of the offspring. Homozygous mice exhibit elevated expression of proinflammatory cytokines resulting in perinatal death from cachexia (wasting), as well as progressive growth retardation, impaired movement, th ..... For more information please see the full phenotype on the strain data sheet | ||
| 006054 | B6.C-Tg(CMV-cre)1Cgn/J | Repository- Live |
| In this transgenic strain, deletion of loxP-flanked genes occurs in all tissues, including germ cells. The cre gene in this strain is under the transcriptional control of a human cytomegalovirus minimal promoter and is likely to be expressed before implantation during early embryogenesis. It also appears that the cre gene is X-linked since transgene transmission through males is restricted to female offspring. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available. | ||
| 006366 | B6.Cg-Dicer1tm1Bdh/J | Repository- Live |
| These mice contain loxP sites on either side of exon 23. Homozygous mice are viable and fertile with no gross phenotypic or behavioral abnormalities. Expression of the targeted allele is indistinguishable from wild-type despite the frt-flanked neomycin cassette. Cre-mediated recombination (resulting in deletion of exon 23) in the germline leads to developmental arrest at embryonic day 7.5 (E7.5). Tissue specific deletion has been shown to result in loss of microRNA (miRNA) processing. Mutant mice can be used to generate cell/tissue-specific deletions of the endogenous gene for applications in embryonic development, translation, protein processing and miRNA/siRNA regulation of gene expression. For example, when crossed to a strain expressing Cre recombinase in mesenchyme (see Stock No. 005584), this mutant mouse strain may be useful in studies of limb morphogenesis. When bred to a strain expressing Cre recombi ..... | ||
| 007914 | B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | Repository- Live |
| Ai14 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE::deltaNeo conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. Ai14 mice are not expected to express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre is expected to be detectable by fluorescence and mRNA (in situ hybridization). These Ai14 mice are useful as a Cre reporter strain; expressing the red fluorescent protein variant, tdTomato, following Cre-mediated recombination.
For cha ..... | ||
| 012567 | B6.Cg-Gt(ROSA)26Sortm27.1(CAG-COP4*H134R/tdTomato)Hze/J | Repository- Live |
| Ai27D (or Ai27Δneo) mice heterozygous for the Rosa-CAG-LSL-hChR2(H134R)-tdTomato-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream hChR2(H134R)-tdTomato fusion gene. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, hChR2(H134R)-tdTomato expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the hChR2(H134R)-tdTomato fusion protein. The donating investigator reports that Ai27D mice do not express hChR2(H134R)-tdTomato prior to introduction of Cre recombinase. Fusion protein expression following exposure to cre can be detected by tdTomato fluorescence and mRNA (in situ hybridization) (and presumably by antibody staining (immunohistochemistry); althoug ..... For more information please see the full phenotype on the strain data sheet | ||
| 007903 | B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J | Repository- Live |
| Ai3 mice hemizygous for this Rosa-CAG-LSL-EYFP-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream enhanced yellow fluorescent protein (EYFP). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of EYFP. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, EYFP expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai3 mice do not express EYFP prior to introduction of Cre recombinase and EYFP expression following exposure to cre can be detected by fluorescence, mRNA (in situ hybridization) and antibody staining (immunohistochemistry). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-medi ..... For more information please see the full phenotype on the strain data sheet | ||
| 007906 | B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J | Repository- Live |
| Ai6 mice hemizygous for this Rosa-CAG-LSL-ZsGreen1-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream enhanced green fluorescent protein (ZsGreen1). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of ZsGreen1. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, ZsGreen1 expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai6 mice do not express ZsGreen1 prior to introduction of Cre recombinase and ZsGreen1 expression following exposure to cre can be detected by fluorescence and mRNA (in situ hybridization). Bright fluorescence is observed mainly in cell bodies. Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the report ..... For more information please see the full phenotype on the strain data sheet | ||
| 007909 | B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J | Repository- Live |
| Ai9 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai9 mice do not express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre can be detected by fluorescence and mRNA (in situ hybridization). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-mediated recombination if ..... For more information please see the full phenotype on the strain data sheet | ||
| 016231 | B6.Cg-Msh2tm2.1Rak/J | Repository- Live |
| The Msh2LoxP allele has loxP sites flanking exon 12 of the mutS homolog 2 (E. coli) [Msh2] gene. Homozygous Msh2LoxP mice are viable and fertile with no observed abnormalities. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding a portion of the essential ATPase domain of MSH2 protein deleted in the cre-expressing tissue(s). These Msh2LoxP mice may be useful in generating tissue-specific MSH2 deletions for studying DNA mismatch repair (single-nucleotide and insertion/deletion mismatches), as well as tumor development.
For example, when Msh2LoxP mice are bred to a strain expressing Cre recombinase in embryonic tissues (EIIA-Cre; see Stock Nos. 003314 or 003724), the resulting mice with pan deletion of MSH2 exhibit high inciden ..... | ||
| 012358 | B6.Cg-Pvalbtm1.1(cre)Aibs/J | Repository- Live |
| Mice heterozygous for the Pvalb-2A-Cre allele are viable and fertile, with a viral 2A oligopeptide that mediates ribosomal skipping and a Cre recombinase gene inserted immediately downstream of the parvalbumin translational STOP codon. As such, Pvalb-2A-Cre mice have both endogenous gene and Cre recombinase expression directed to Pvalb-expressing cells by the endogenous promoter/enhancer elements of the parvalbumin locus. When Pvalb-2A-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Pvalb-expressing cells in the offspring. Specifically, the donating investigator reports cre activity in scattered interneuron populations in the cortex and hippocampus, as well as neuronal populations in other brain regions, resembling the Pvalb expression pattern. Additional reporter gene expression is seen in cortical layer 5 neurons, which are unlikely to be interneurons. ..... For more information please see the full phenotype on the strain data sheet | ||
| 005622 | B6.Cg-Shhtm1(EGFP/cre)Cjt/J | Repository- Live |
| This strain expresses a fusion product involving Enhanced Green Fluorescent Protein (EGFP) and Cre recombinase from the endogenous Shh locus. EGFP and cre expression are consistent with the endogenous gene. Fluorescence is detected in the distal posterior region of the limb buds of embryos aged embryonic day 10 to 12 and colocalizes with the endogenous gene product (mRNA).
The donating investigator reports that it is not uncommon for a mosaic expression pattern to be exhibited when the allele is inherited through the female germline. It is recommended that this allele be passed through the male germline when conducting experiments involving cre-induced recombination. Mice homozygous for the mutation develop a limited limb skeleton and lack digit 2. Homozygous mice are not viable or fertile. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
This mutant mouse strain may be useful in studie ..... For more information please see the full phenotype on the strain data sheet | ||
| 007484 | B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ | Repository- Live |
| On an albino background the X-linked transgene Tg(Tyr)3412ARpw permits visual identification of XX versus XY as early as embryonic day 10.5. This strain is segregating for Tg(Tyr)3412ARpw and homozygous for Tyrc-2J so the individuals not carrying Tg(Tyr3412)ARpw are albino. Because Tg(Tyr)3412ARpw inserted into the X Chromosome, breeding a carrier male with a noncarrier (wild-type) female results in embryos in which all XX individuals develop eye pigment, due to the Tg(Tyr)3412ARpw inherited from their father, while all XY individuals have non-pigmented eyes, having inherited a wild-type X Chromosome from their mother.
This strain is also homozygous for Tg(SryEGFP)92Ei. This reporter transgene consists of a 5 prime regulatory segment of the Sry gene driving EGFP. This transgene is expressed in the pre-support cell lineage (pre-sertoli and pre-granulosa cells) of the fetal genital ridge (Albrecht and Eicher, 2001) and in discrete areas the adult male but not ..... | ||
| 006881 | B6.Cg-Tg(Aqp2-cre)1Dek/J | Repository- Live |
| Mice hemizygous for this AQP2-Cre transgene are viable and fertile. Transgenic cre activity, directed by the mouse aquaporin 2 promoter, is observed in kidney cells (collecting duct) and testes (sperm). When bred with mice containing a loxP-flanked sequence of interest, cre-mediated recombination will result in deletion of the flanked sequence. In such breedings, maternal inheritance of the transgene is recommended for kidney-specific recombinase activity as males express cre in sperm as well as kidney tissues. These AQP2-Cre mice may be used to generate conditional mutations in the renal collecting duct for studying nephrology, physiology, metabolism, or type II diabetes. In addition, cre expression in sperm may be useful in generating conditional mutations in multiple or all tissues in the resulting offspring. | ||
| 005317 | B6.Cg-Tg(BAT-lacZ)3Picc/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These "BAT-GAL" transgenic mice are a reporter strain that express beta-galactosidase in the presence of activated beta-catenin. The transgene expresses the lacZ gene under the control of a regulatory sequence consisting of seven consensus LEF/TCF-binding motifs upstream of the Xenopus siamois gene minimal promoter. Transgenic mice display beta-galactosidase activity beginning at embryonic day 6.0 in the posterior side of the proximal epiblast. Beta-galactosidase expression is detectable in the posterior primitive streak and node at gastrulation, and progresses to the paraxial mesoderm and notochord. Beta-galactosidase activity in developing and adult nervous tissue mimics the pattern of Wnt signaling. When bred to other mutant strains, this reporter strain may be useful for identifying mutations that affect the Wnt-signalling pa ..... For more information please see the full phenotype on the strain data sheet | ||
| 006051 | B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J | Repository- Live |
| Mice homozygous for this Actb-DsRed.T3 transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the red fluorescent protein variant DsRed.MST under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. All tissues of homozygotes fluoresce red. Mice hemizygous for the transgene express red fluorescent protein less intensely than homozygotes. Expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description as published results bec ..... | ||
| 005884 | B6.Cg-Tg(CAG-mRFP1)1F1Hadj/J | Repository- Live |
| Mice homozygous for the transgene exhibit robust and widespread expression of monomeric red fluorescent protein-1 (mRFP1) in embryonic stem cells, embryos, and adults. Unlike tetrameric or dimeric fluorescent proteins, high levels of mRFP1 expression do not affect cell morphology, developmental potential, viability, and fertility of homozygous mice. Various optical imaging modalities can be used to visualize mRFP1 expressing cells in culture, in embryos and adult mice. Cells from transgenic mRFP1 mice, sampled along with green and cyan fluorescent cells from other mice, show clearly discernable fluorescence. This mouse may be useful in studies of mRFP1 cell lines, transplantation, embryo chimera experiments, and fluorescent imaging and technology development. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted tha ..... | ||
| 009350 | B6.Cg-Tg(CDX2-cre)101Erf/J | Repository- Live |
| Mice hemizygous for the CDX2P9.5-NLSCre transgene are viable and fertile, with a 9.5 kb human caudal type homeo box 2 (CDX2) promoter/enhancer sequence directing expression of a nuclear-localized Cre recombinase predominantly to colonic epithelium during late gestation and in adult tissues. Specifically, Cre recombinase expression is observed in epithelium from the distal ileum and cecum, and throughout the colon from the crypt base to the luminal surface. Cre recombinase expression is also observed throughout the caudal region of the embryo during early development. When these transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. | ||
| 005359 | B6.Cg-Tg(Camk2a-cre)T29-1Stl/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the Cre recombinase under the control of the mouse calcium/calmodulin-dependent protein kinase II alpha promoter. Cre recombinase expression is detected in the forebrain, specifically to the CA1 pyramidal cell layer in the hippocampus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination occurs in the pyramidal cell layer. | ||
| 012237 | B6.Cg-Tg(Cdh16-cre)91Igr/J | Repository- Live |
| Hemizygous and homozygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These Ksp1.3/Cre transgenic mice express Cre recombinase under the control of the mouse cadherin 16 (Cdh16 or Ksp-cadherin) promoter. Cre recombinase expression follows expression of the endogenous gene and is detected in the epithelial cells of developing nephrons, ureteric bud, mesonephric tubules, Wolffian duct, and Mullerian duct. In the adult mouse expression is limited to the renal tubules especially the collecting ducts, loops of Henle and distal tubules. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in the offspring. This mutant mouse strain may be useful in kidney-specific gene targeting and cell lineage studies. | ||
| 006137 | B6.Cg-Tg(Cdh5-cre)7Mlia/J | Repository- Live |
| Hemizygotes are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. In the differentiated endothelium transgene expression is observed as early as E7.5 and progresses to almost full penetrance by E14.5. In adult mice, uniform cre expression is observed in the endothelium of developing and quiescent vessels of all organs examined, as well as within a subset of hematopoietic cells. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome. These mice may be useful in studies of the cardiovascular system, including angiogenesis, and endothelial and hematopoietic cell lineages. | ||
| 016241 | B6.Cg-Tg(Col1a1-cre/ERT2)1Crm/J | Repository- Live |
| These transgenic mice express a tamoxifen inducible Cre recombinase driven by the 2.3-kb mouse Col1a1, collagen, type I, alpha 1, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in the osteoblasts of most bones and in odontoblasts of teeth in embryonic and postnatal mice. Inducible Cre recombinase activity is detected in the osteoblasts of the long bones of the limbs a ..... For more information please see the full phenotype on the strain data sheet | ||
| 008538 | B6.Cg-Tg(Cspg4-cre/Esr1*)BAkik/J | Repository- Live |
| Mice hemizygous for the NG2CreERTM BAC transgene are viable and fertile, with the mouse NG2 (Cspg4) promoter/enhancer directing expression of a tamoxifen-inducible Cre recombinase (CreERTM). This CreERTM fusion protein is estrogen insensitive, and is only active when it binds the estrogen analog 4-hydroxytamoxifen (OHT) or tamoxifen.
When these NG2CreERTM BAC transgenic mice are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. No background cre activity is reported in the absence of tamoxifen. Following tamoxifen administration, the majority of Cre recombinase activity is reported in NG2-expressing glia (polydendrocytes, oligodendrocyte progenitor cells). The donating investigator specifically reports that tamoxifen-induced Cre recombinase activity is observed throu ..... For more information please see the full phenotype on the strain data sheet | ||
| 007897 | B6.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J | Repository- Live |
| Mice hemizygous for this ROSA26-EGFP BAC transgene are viable and fertile, with widespread EGFP expression in all organs and tissues analyzed. In addition, stable and uniform EGFP expression is observed in differentiated progeny of transplanted hematopoietic stem cells (HSC) isolated from ROSA26-EGFP mice. These ROSA26-EGFP BAC transgenic mice may be useful for fluorescent labeling of cells and tissues both in vivo and in vitro, are suitable for use in histological preparations, and are appropriate as a source of fluorescently labeled donor cell populations (such as tracking hematopoietic cell fate in transplant recipients).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as publis ..... | ||
| 004191 | B6.Cg-Tg(HLA-A/H2-D)2Enge/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express an interspecies hybrid class I MHC gene, AAD, which contains the alpha-1 and alpha-2 domains of the human HLA-A2.1 gene and the alpha-3 transmembrane and cytoplasmic domains of the mouse H-2Dd gene, under the direction of the human HLA-A2.1 promoter. Immunodetection of the HLA-A2.1 recombinant transgene established that expression was at equivalent levels to endogenous mouse class I molecules. The mouse alpha-3 domain expression enhances the immune response in this system. Compared to unmodified HLA-A2.1, the chimeric HLA-A2.1/H2-Dd MHC Class I molecule mediates efficient positive selection of mouse T cells to provide a more complete T cell repertoire capable of recognizing peptides presented by HLA-A2.1 Class I molecules. The peptide epitopes presented and recognized by mouse T cells in the ..... For more information please see the full phenotype on the strain data sheet | ||
| 007742 | B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J | Repository- Live |
| Mice hemizygous for the smMHC/Cre/eGFP transgene (smMHCCre/eGFP) are viable and fertile, with the smooth muscle myosin heavy chain (smMHC or Myh11) promoter directing bicistronic Cre and EGFP protein expression to smooth muscle cells during development as well as in the adult mouse. Hemizygotes from founder line SMCG2 (SM2Cre/GFP) display intense EGFP fluorescence restricted to vascular and nonvascular smooth muscle, with strong concordance between cre expression and EGFP fluorescence (verifying the use of fluorescence as a marker for conditional gene recombination). When bred with mice containing a loxP-flanked sequence of interest, the resulting offspring can have Cre-mediated recombination of the flanked sequence in smooth muscle. Homozygotes are viable and fertile, with smaller litter sizes and a higher incidence of perinatal mortality. These smMHC/Cre/eGFP transgenic mice may be useful in studies utilizing "Cre-lox" technology or fluoresc ..... For more information please see the full phenotype on the strain data sheet | ||
| 010536 | B6.Cg-Tg(Pcp2-cre)3555Jdhu/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse Purkinje cell protein (Pcp2). Cre recombinase expression is detected in Purkinje cells of the cerebellar folia and retinal bipolar cells. Expression was not found in spinal cord, heart, liver, kidney or cornea. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the offspring. This mutant mouse strain may be useful in studies of the nervous system, particularly Purkinje cells and retinal bipolar cells. | ||
| 008827 | B6.Cg-Tg(Prdm1-cre)1Masu/J | Repository- Live |
| These transgenic mice express Cre recombinase under the control of the mouse Prdm1 (PR domain containing 1, with ZNF domain; Blimp1) promoter. Cre-mediated recombination is detected in 55-76% of primordial germ cells when this strain is crossed with Gt(ROSA)26-GFP reporter mice. Expression is also seen in plasma cells. These mice may be useful for generating tissue-specific targeted mutants for studies of development and germ cell fate. | ||
| 007902 | B6.Cg-Tg(RP23-268L19-EGFP)2Mik/J | Repository- Live |
| Mice hemizygous for the ChAT(BAC)-eGFP (ChATBAC-eGFP) transgene are viable and fertile, with the endogenous choline acetyltransferase (ChAT) transcriptional regulatory elements (cholinergic gene locus) directing enhanced green fluorescent protein (EGFP) protein expression during development as well as in the adult mouse. As such, EGFP is expressed in central and peripheral cholinergic neurons, including cell bodies and processes of the somatic motor, somatic sensory, and parasympathetic nervous system in gastrointestinal, respiratory, urogenital, cardiovascular, and other peripheral organ systems and neuromuscular junctions. These ChAT(BAC)-eGFP transgenic mice allow fluorescent visualization of cholinergic elements of the central and peripheral nervous system and may be useful for studying cholinergic neurotransmission and neuromuscular coupling. | ||
| 006235 | B6.Cg-Tg(SFTPC-rtTA)5Jaw/J | Repository- Live |
| Mice that are hemizygous for this transgenic insert are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human SFTPC, surfactant, pulmonary-associated protein C, promoter. In situ hybridization detects rtTA gene product (mRNA) in lung peripheral epithelial cells from adult mice and 15 postconception day aged embryos from doxycycline treated dams. Induction of transgene expression is detected as early as postconception day 12.5 when the pregnant female is treated with doxycycline. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is ..... For more information please see the full phenotype on the strain data sheet | ||
| 008454 | B6.Cg-Tg(Sox2-cre)1Amc/J | Repository- Live |
| Mice hemizygous for the Sox2Cre transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse SRY-box containing gene 2 promoter. When these transgenic mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in Sox2-expressing tissues in the offspring. Specifically, Cre recombinase activity is detected in the epiblast cells at embryonic day 6.5, with little or no activity in other cells at gastrulation. Some activity is also detected in extra embryonic derivatives of the epiblast, the yolk sac mesoderm and amnion. No Cre recombinase activity is detected in primitive endoderm derived tissues, visceral endoderm. The phenotype of homozygous mice has not been characterized to date (April 2011). These Sox2Cre transgenic may be useful for generating epiblast-derived specific ..... For more information please see the full phenotype on the strain data sheet | ||
| 006361 | B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J | Repository- Live |
| Mice hemizygous for this Osx1-GFP::Cre transgene are viable and fertile.
The transgene carries both tTA under the regulation of the osterix (Sp7) promoter and, just downstream, a tetracycline responsive element (TRE; tetO)-controlled GFP/Cre fusion protein. In the absence of the tetracycline analog doxycycline, EGFP-Cre fusion protein expression is restricted to the osteoblast lineage throughout embryonic and early postnatal development. Fusion protein activity is largely absent from chondrocytes. When these transgenic animals are mated to transgenic strains that carry loxP-flanked (floxed) conditional alleles, Cre-mediated recombination of the floxed allele in the double mutant animals is placed under the regulation of doxycycline (dox) such that dox adminstration prevents fusion protein expression and recombination. The donating investigator suggests that the mice be maintained on dox-treated water to avoid incidental effects of tTA expression (e.g., malocclusion). Th ..... For more information please see the full phenotype on the strain data sheet | ||
| 010905 | B6.Cg-Tg(Sry)2Ei Srydl1Rlb/ArnoJ | Repository- Live |
| The dl1Rlb allele (Y-) is an 11 kb deletion in the sex determining region of the Y chromosome, Sry , XY- mice (with ovaries) with this mutation are phenotypic gonadal females, although they lose germ cells and cease estrous cycling earlier in life. The donating investigator indicates that XY- mice generally infertile on the C57BL/6 background . XX mice carrying the Tg(Sry)2Ei transgene are phenotypic gonadal males (with testes), although they lack sperm and have smaller testes than normal males.
When the two mutations are combined, testis determination is transferred from the Y chromosome to an autosome. Mating the carrier male to a C57BL/6J female produces four "core" genotypes that can be used as a model to investigate relationships between sex chromosome complement (XX or XY) and gonadal type that influences phenotypic characteristics. The four genotypes produced are two types of gonadal females (XX, XY-), and two types ..... For more information please see the full phenotype on the strain data sheet | ||
| 004128 | B6.Cg-Tg(Tek-cre)12Flv/J | Repository- Live |
| These transgenic mice express Cre recombinase under the direction of the receptor tyrosine kinase Tek promoter/enhancer, which has been shown to provide uniform expression in endothelial cells during embryogenesis and adulthood. Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity results in the deletion of loxP flanked targets in the female germline as well as in endothelial cells and hematopoietic cells. A low frequency of deletion events are also observed by inheritance from the male germline. | ||
| 008863 | B6.Cg-Tg(Tek-cre)1Ywa/J | Repository- Live |
| These transgenic mice express Cre recombinase under the direction of the receptor tyrosine kinase Tek (Tie2) promoter/enhancer, which has been shown to provide uniform expression in endothelial cells during embryogenesis and adulthood. Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity results in the deletion of loxP flanked targets in vascular endothelial cells. | ||
| 007901 | B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J | Repository- Live |
| These Thy1-Brainbow 1.0 (line H) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Prior to Cre-mediated recombination, the fluorescent protein immediately adjacent to the promoter, tdimer2(12) (RFP), is expressed in peripheral and central neurons. When bred to Cre recombinase expressing mice, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): tdimer2(12) (RFP) (no recombination), mYFP, or mCerulean (CFP). A palmitoylation sequence tethers the mYFP and mCerulean (CFP) to the membrane, allowing clear labeling of axonal processes, while cytoplasmic tdimer2(12) (RFP) better labeled neuronal cell bodies and dendrites. Integration of tandem transgen ..... For more information please see the full phenotype on the strain data sheet | ||
| 007911 | B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J | Repository- Live |
| These Thy1-Brainbow 1.1 (line M) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Although the fluorescent protein immediately adjacent to the promoter, Kusabira-Orange (kOFP), was designed to be expressed prior to Cre-mediated recombination, basal kOFP expression is not observed in mouse tissues. When bred to Cre recombinase expressing mice, however, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): mCerulean (CFP), mYFP, or mCherry (RFP). The resulting fluorescent protein expression is observed in astrocytes of all areas of the brain and spinal cord, as well as dentate gyrus granule cells. A palmitoylation sequence tethers the mCherry (RFP), ..... For more information please see the full phenotype on the strain data sheet | ||
| 007921 | B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J | Repository- Live |
| These Thy1-Brainbow 2.1 (line R) transgenic mice are viable and fertile. The mice possess two invertible DNA segments (four fluorescent protein sequences in total) uniquely positioned in tandem and flanked with LoxP sites to generate a larger number of recombination outcomes; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Although the fluorescent protein immediately adjacent to the promoter, hrGFPII (with nuclear localization signal), was designed to be expressed prior to Cre-mediated recombination, basal hrGFPII expression may not be observed in mouse tissues. When bred to Cre recombinase expressing mice, however, the resulting offspring can have one of three different inversions for each transgene in each cell of the cre expressing tissue(s). In addition, two excision events may reduce the construct to one of two single invertible DNA segments which can continue to invert as long as cre is present. These different recomb ..... For more information please see the full phenotype on the strain data sheet | ||
| 007919 | B6.Cg-Tg(Thy1-EGFP)OJrs/GfngJ | Repository- Live |
| Mice hemizygous for the Thy1-GFP transgene are viable and fertile with enhanced green fluorescent protein (EGFP) expression under the control of a modified Thy1 promoter region (containing the sequences required for neuronal expression but lacking the sequences required for expression in non-neural cells). Thy1-GFP mice derived from founder line O express EGFP in subsets of neurons within specific populations; providing a bright, vital Golgi-like stain. High (>80&) EGFP expression is observed in motor axons and cerebellar mossy fibers. Low (<10%) EGFP expression is observed in retinal ganglion cells and lumbar dorsal root ganglion. Sparse EGFP-labeling is also observed in neurons from multiple cortical layers. These Thy1-GFP line O transgenic mice may be useful for fluorescent labeling of neural tissues; especially motor axons and cerebellar mossy fibers, as well as intense, yet sparse, labeling of a variety of cortical neurons.
This strain is one of many from the same transgene ..... | ||
| 003709 | B6.Cg-Tg(Thy1-YFP)16Jrs/J | Repository- Live |
| These mice express spectral variants of GFP (yellow-YFP) at high levels in motor and sensory neurons, as well as in subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. The transgenic insert used to make this strain is identical to that used in the construction of Stock No. 003782. This line provides a strong and specific vital marker for axons; fluorescence is also stable to mild aldehyde fixation. Expression is strong from a mid-gestational stage into adulthood. Availability of multiple spectral variants is useful for double-labeling applications, or breeding to other CFP/GFP lines. | ||
| 015805 | B6.Cg-Tg(UBC-GFP,-TVA)1Clc/J | Repository- Live |
| The cTVA transgene contains the human ubiquitin C (UBC) promoter/enhancer elements drive expression of a loxP-flanked green fluorescent protein (GFP) and polyadenylation sequence, followed by an avian specific retroviral receptor (TVA) gene derived from quail. Hemizygous mice are viable and fertile. UBC is a polyubiquitin precursor responsible for the regulation of cell signaling pathways upon conjugation with various proteins. In this mutant mouse, GFP is expressed in UBC-expressing cells. When bred to mice that express Cre recombinase, offspring will have the floxed-GFP-STOP cassette deleted in the cre-expressing tissue(s), resulting in TVA overexpression in UBC-expressing cells. Upon expression of TVA, these cells are capable of binding viruses with the envelope protein of avian sarcoma/leukosis virus subtype A (ASLV-A). These cTVA mice may be useful for infection by retroviruses, rabies virus, and other viruses with ASLV-A ..... For more information please see the full phenotype on the strain data sheet | ||
| 015806 | B6.Cg-Tg(UBC-GFP,-TVA)2Clc/J | Repository- Live |
| The cTVA transgene contains the human ubiquitin C (UBC) promoter/enhancer elements drive expression of a loxP-flanked green fluorescent protein (GFP) and polyadenylation sequence, followed by an avian specific retroviral receptor (TVA) gene derived from quail. Hemizygous mice are viable and fertile. UBC is a polyubiquitin precursor responsible for the regulation of cell signaling pathways upon conjugation with various proteins. In this mutant mouse, GFP is expressed in UBC-expressing cells. When bred to mice that express Cre recombinase, offspring will have the floxed-GFP-STOP cassette deleted in the cre-expressing tissue(s), resulting in TVA overexpression in UBC-expressing cells. Upon expression of TVA, these cells are capable of binding viruses with the envelope protein of avian sarcoma/leukosis virus subtype A (ASLV-A). These cTVA mice may be useful for infection by retroviruses, rabies virus, and other viruses with ASLV-A ..... For more information please see the full phenotype on the strain data sheet | ||
| 015807 | B6.Cg-Tg(UBC-GFP,-TVA)3Clc/J | Repository- Live |
| The cTVA transgene contains the human ubiquitin C (UBC) promoter/enhancer elements drive expression of a loxP-flanked green fluorescent protein (GFP) and polyadenylation sequence, followed by an avian specific retroviral receptor (TVA) gene derived from quail. Hemizygous mice are viable and fertile. UBC is a polyubiquitin precursor responsible for the regulation of cell signaling pathways upon conjugation with various proteins. In this mutant mouse, GFP is expressed in UBC-expressing cells. When bred to mice that express Cre recombinase, offspring will have the floxed-GFP-STOP cassette deleted in the cre-expressing tissue(s), resulting in TVA overexpression in UBC-expressing cells. Upon expression of TVA, these cells are capable of binding viruses with the envelope protein of avian sarcoma/leukosis virus subtype A (ASLV-A). These cTVA mice may be useful for infection by retroviruses, rabies virus, and other viruses with ASLV-A ..... For more information please see the full phenotype on the strain data sheet | ||
| 009614 | B6.Cg-Tg(Wfs1-cre/ERT2)2Aibs/J | Repository- Live |
| Hemizygous Wfs1-Tg2-CreERT2 mice are viable and fertile. As the Cre-ERT2 fusion gene is under control of the Wfs1 promoter/enhancer regions within the BAC transgene, cre activity is directed to cortex, hippocampus, and cerebellum only following tamoxifen administration. The donating investigators report that Cre recombinase expression for this Wfs1-Tg2-CreERT2 line is more restricted than the Wfs1-Tg3-CreERT2 line (Stock No. Stock No. 009103). The donating investigators may not have assessed expression in tissues other than brain. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only ga ..... For more information please see the full phenotype on the strain data sheet | ||
| 009107 | B6.Cg-Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J | Repository- Live |
| In 2011, Stock No. 009107 at The Jackson Laboratory Repository was confirmed to harbor the expected Wnt1-Cre transgene, as well as the originally co-injected Wnt1-GAL4 transgene. The strain phenotype description below has been updated accordingly.
When homozygous for both co-injected transgenes, Wnt-1/GAL4/cre-11 transgenic mice are viable, fertile, normal in size and do not display any gross physical abnormalities. Of note for Wnt-1/GAL4/cre-11 transgenic mice on the original mixed genetic background, the donating investigator (Dr. Epstein) reported that homozygous mice may be lethal as some offspring from transgenic parents die around two months of age. Both Cre recombinase and the GAL4 transcriptional activator are expressed under the direction of wingless-related MMTV integration site 1 (Wnt1) promoter/regulatory sequences. Cre recombinase activity is detected in the Wnt1 pattern of expression: in the midbrain by 8.5 dpc and, after neural ..... | ||
| 005657 | B6.FVB(129)-Tg(Myh6-cre/Esr1*)1Jmk/J | Repository- Live |
| The alpha-MHC-MerCreMer transgene has the mouse Myh6 promoter (myosin, heavy polypeptide 6, cardiac muscle, alpha; alpha-MHC) directing expression of a tamoxifen-inducible Cre recombinase (MerCreMer) to juvenile and adult cardiac myocytes. Mice homozygous for the alpha-MHC-MerCreMer transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre recombinase expression in heart tissue is confirmed by western blot. Southern blot confirmed heart cell specificity compared to brain, kidney, lung, liver, and skeletal muscle. Insertion of this transgene and its protein show no changes in echocardiography, heart mass or pathology, or hypertrophy marker genes compared to nontransgenic littermates. Of note, the MerCreMer double fusion protein has substantially greater Cre recombinase activity with less promiscuity compared with the CreMer single fusion protein. When alpha-MHC-MerCreMer transgenic mice are bred with mice containing For more information please see the full phenotype on the strain data sheet | ||
| 004586 | B6.SJL-Tg(Vil-cre)997Gum/J | Repository- Live |
| Mice hemizygous for this transgene express Cre recombinase under the direction of the mouse villin 1 promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in villi and crypt cells of the small and large intestines, closely patterning the endogenous gene expression. The Donating Investigator indicates that expression is generally continuous, but that a small amount of mosaicism is noted in the colon. Onset of transgene expression is at 12.5 dpc, which is delayed from the endogenous mouse Vil1 gene expression onset of 9.0 dpc. Hemizygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of intestinal organogenesis. | ||
| 010531 | B6;129-Bmi1tm1(cre/ERT)Mrc/J | Repository- Live |
| These targeted mutant mice carry tamoxifen-inducible Cre under the transcriptional control of the mouse Bmi1 (Bmi1 polycomb ring finger oncogene) promoter specifically expressed in discrete cells located near the bottom of crypts in the small intestine (predominantly four cells above the base of the crypt). When crossed with a strain containing a loxP-flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. When crossed with a floxed reporter strain, lineage tracing of Bmi1-expressing cells is possible. | ||
| 014125 | B6;129-Flnctm1Lmk/J | Repository- Live |
| The Flnc Δ41-48 mutant allele has the last eight exons (exons 41-48) of the filamin C (FLNc) locus deleted. The deleted region encodes the last five filamin-like repeats (including the dimerization domain) and the Hinge 2 domain of FLNc. As such, the Flnc Δ41-48 allele expresses a truncated mRNA at reduced levels compared to the wildtype allele in limb muscle and heart tissues. Western blot analysis on limb muscle protein lysates shows that the Flnc Δ41-48 allele expresses a truncated protein at very low levels. Heterozygous mice are viable and fertile with no reported abnormalities. Mice homozygous for the Flnc Δ41-48 allele die at birth. At embryonic day (E)18.5, homozygous mice born via Cesarean section are able to take several short breaths but exhibit respiratory distress (failure to inflate lungs) and die shortly thereafter. While the hearts of the homozygotes appear normal and unaffected, skeletal muscles exhibit severe abnor ..... For more information please see the full phenotype on the strain data sheet | ||
| 005549 | B6;129-Pax3tm1(cre)Joe/J | Repository- Live |
| This strain expresses Cre recombinase from the endogenous Pax3 locus. Expression of the targeted gene product (mRNA and protein) mimics endogenous gene expression as detected by in situ hybridization and immunohistochemistry of homozygous embryos aged E12.5. No endogenous Pax3 gene product (protein) is detected in homozygotes and approximately one half of the endogenous gene product (protein) is detected in heterozygotes by Western blot analysis. Cre recombinase expression is detected in the dorsal neural tube and somites of E9 to 11.5 embryos and in the cardiac neural crest cells and colonic epithelia of E11.5 embryos. Recombination occurs in neural crest and somite derivatives of later gestation embryos. Homozygous mice have an embryonic lethal phenotype, failing to develop past embryonic day 18.5. At age E13.5 homozygous embryos display severe cardiac and neural tube defects (exencephaly), absent limb musculature and reduced or absent dorsal root ganglia. Heterozygous ..... For more information please see the full phenotype on the strain data sheet | ||
| 009600 | B6;129-Six2tm3(EGFP/cre/ERT2)Amc/J | Repository- Live |
| While heterozygous mice are viable and fertile with no reported abnormalities, homozygous mice die shortly after birth. The Six2GCE "knock-in" allele both abolishes Six2 gene function and expresses an eGFPCreERT2 fusion protein (EGFP and creERT2 fusion protein) from the Six2 promoter/enhancer elements. While EGFP immunofluorescence is observed in nephron progenitor population cap mesenchyme from the onset of metanephric kidney development, Cre-ERT2 fusion gene activity is inducible; observed in the same cells only following tamoxifen administration. As such, when Six2GCE mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Six2-expressing cells of the offspring.
The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand ..... | ||
| 008529 | B6;129P-Tg(Neurog1-cre/ERT2)1Good/J | Repository- Live |
| These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse neurogenin 1, Neurog1, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/ESR1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in the cortex, hippocampus, thalamus, hypothalamus and cochlear-vestibular ganglion. Hemizygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnorma ..... For more information please see the full phenotype on the strain data sheet | ||
| 015854 | B6;129P2-Foxl2tm1(GFP/cre/ERT2)Pzg/J | Repository- Live |
| These Fox L2-GCE knock in mice utilize a tamoxifen inducible Cre-mediated recombination system. A GFP-Cre/ERT2 cassette (GCE) was inserted into the Foxl2, forkhead box L2, locus. No green fluorescence was detected by direct fluorescence microscopy, however, immunohistochemical analysis revealed GFP expression in embryos, 15.5 embronic days of age. Tamoxifen administration induces Cre recombination in a small population of granulosa precursor cells in the medulla of the developing ovary of embryos, 15.5 embronic days of age. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 009336 | B6;129P2-Map1lc3btm1Mrab/J | Repository- Live |
| Homozygous (LC3β-/-) mice are viable and fertile, harboring an IRES Tau-lacZ/floxed PGK-neo allele that disrupts exons III-IV of the targeted gene. No LC3β protein expression from the targeted locus is detected in developing head and trunk tissues at embryonic day (E)14.5 or E18.5. No lacZ expression is reported. While homozygotes have a mild survival advantage in the absence of feeding (suggesting compensatory increase(s) in other autophagy proteins), no other overt phenotype is reported. LC3β-/- mouse embryonic fibroblasts (MEFs) exhibit reduced fibronectin synthesis but retain normal levels of fibronectin protein. These LC3β-mutant mice may be useful in studying the role of murine light chain RNA-binding proteins in fibronectin regulation as well as starvation and autophagy. | ||
| 006847 | B6;129P2-Mecp2tm1Bird/J | Repository- Live |
| These mice possess two functional loxP sites flanking exons 3-4 of the targeted gene on the X chromosome. Homozygous females and hemizygous males are viable and fertile. Northern blot analysis showed the expected mature transcript from the Mecp2lox locus. Also detected was an unspliced beta-globin transcript that was introduced into the locus as part of the targeting vector. When these mutant mice are bred to mice that express cre recombinase, resulting offspring will have exons 3-4 deleted in the cre-expressing tissue(s). Mice with this X-linked floxed mutation may be useful in neurological and developmental studies of Rett syndrome. For example, when crossed to a strain expressing Cre recombinase in nervous tissue (see Stock No. 003771), this mutant mouse strain develops a neurological phenotype that mimics Rett syndrome. When bred to a strain expressing Cre recombinase in embryonic fo ..... | ||
| 012336 | B6;129P2-Terf1tm2.1Tdl/J | Repository- Live |
| These TRF1F mice harbor loxP sites flanking exon 1 (encoding the translation start site) of the telomeric repeat binding factor 1 locus. Telomeres serve a dual role in protecting the chromosome ends from degradation/repair activities and in intracellular signaling for regulating cell proliferation. Mammalian telomeres are formed by tandem TTAGGG sequence repeats bound by a specialized complex of six telomere-associated proteins called the shelterin complex. As TRF1 is one of the components of shelterin, these TRF1F mutant mice may be useful in generating conditional mutations for studying the shelterin complex of telomeres, telomere maintenance, chromosomal stability, cancer, and aging. | ||
| 012569 | B6;129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J | Repository- Live |
| Ai32 mice heterozygous for the Rosa-CAG-LSL-ChR2(H134R)-EYFP-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream ChR2(H134R)-EYFP fusion gene. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, ChR2(H134R)-EYFP expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the ChR2(H134R)-EYFP fusion protein. The donating investigator reports that Ai32 mice do not express ChR2(H134R)-EYFP prior to introduction of Cre recombinase. Fusion protein expression following exposure to cre can be detected by EYFP fluorescence (and presumably by mRNA (in situ hybridization) and antibody staining (immunohistochemistry); although this was not tested by the donating investigator). ..... For more information please see the full phenotype on the strain data sheet | ||
| 012570 | B6;129S-Gt(ROSA)26Sortm34.1(CAG-Syp/tdTomato)Hze/J | Repository- Live |
| Ai34D (or Ai34Δneo) mice heterozygous for the Rosa-CAG-LSL-Synaptophysin-tdTomato-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream Synaptophysin-tdTomato fusion gene. The Synaptophysin-tdTomato fusion protein is composed of near-full-length mouse synaptophysin protein fused in-frame to the amino terminus of the tdTomato fluorescent protein. Because the CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, Synaptophysin-tdTomato expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the Synaptophysin-tdTomato fusion protein. The donating investigator reports that Ai34D mice do not express Synaptophysin-tdTomato prior to introduction of Cre recombinase. Following exposure to ..... For more information please see the full phenotype on the strain data sheet | ||
| 012735 | B6;129S-Gt(ROSA)26Sortm35.1(CAG-AOP3/GFP)Hze/J | Repository- Live |
| Ai35D (or Ai35Δneo) mice heterozygous for the Rosa-CAG-LSL-Arch-GFP-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream Arch-GFP fusion gene (see below for detailed description of Arch-GFP). Because the CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, Arch-GFP expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the Arch-GFP fusion protein.
The donating investigator reports that Ai35D mice do not express Arch-GFP prior to introduction of Cre recombinase. Fusion protein expression following exposure to cre can be detected by GFP fluorescence and mRNA (in situ hybridization) [and presumably by antibody staining (immunohistochemistry); although this was not test ..... For more information please see the full phenotype on the strain data sheet | ||
| 014538 | B6;129S-Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J | Repository- Live |
| Ai38 mice heterozygous for the Rosa-CAG-LSL-GCaMP3-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream GCaMP3 fusion gene (see below for detailed description of GCaMP3). Because the CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, GCaMP3 expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the fluorescent calcium indicator protein, GCaMP3. The donating investigator reports that Ai38 mice do not express GCaMP3 prior to introduction of Cre recombinase. Following exposure to Cre recombinase, GCaMP3 expression (EGFP fluorescence) is detected in the cre-expressing tissues. In the absence of calcium binding, low EGFP fluorescence is reported. Following calcium binding (s ..... For more information please see the full phenotype on the strain data sheet | ||
| 014539 | B6;129S-Gt(ROSA)26Sortm39(CAG-HOP/EYFP)Hze/J | Repository- Live |
| Ai39 mice heterozygous for the Rosa-CAG-LSL-eNpHR3.0-EYFP-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream eNpHR3.0-EYFP fusion gene (see below for detailed description of eNpHR3.0-EYFP). Because the CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, eNpHR3.0-EYFP expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the eNpHR3.0-EYFP fusion protein. The donating investigator reports that Ai39 mice do not express eNpHR3.0-EYFP prior to introduction of Cre recombinase.
Fusion protein expression following exposure to cre can be detected by EYFP fluorescence and mRNA (in situ hybridization) [and presumably by antibody staining (immunohistochemistry); although this was ..... For more information please see the full phenotype on the strain data sheet | ||
| 010983 | B6;129S-Id3tm1Pzg/J | Repository- Live |
| These mutant mice express Red Fluorescent Protein from the endogenous Id3 (inhibitor of DNA binding 3) locus. Strong red fluorescence is observed in the kidney. Red fluorescence is also detected in the urogenital system of E15.5 heterozygous embryos. Fluorescence mimics the endogenous expression pattern. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous.
This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 010618 | B6;129S-Jag1tm2Grid/J | Repository- Live |
| These mice possess loxP sites on either side of exon 4, which encodes the DSL (Delta-Serrate-Lag2) domain, of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in the cre-expressing tissue(s). The exon 4-deleted allele produces a nonfunctional JAG1 protein.
When bred to a strain with Cre recombinase expression during development in the telencephalon and discrete head structures, such as the otocyst (see Stock No. 006084 for example), this mutant mouse strain may be useful in studies of developmental inner ear defects. When bred to a strain with Cre recombinase expression in endothelial cells during embryogenesis and adulthood (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 010686 | B6;129S-Snai1tm2Grid/J | Repository- Live |
| These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 and 3 deleted in the cre-expressing tissue(s). | ||
| 010987 | B6;129S-Sox18tm1(GFP/cre/ERT2)Pzg/J | Repository- Live |
| These Sox18-GCE mutant mice have a tamoxifen inducible Cre-mediated recombination system. A GFP-Cre/ERT2 cassette (GCE) was inserted into the Sox18, SRY-box containing gene 18, locus. Tamoxifen administration induces Cre recombination. Green fluorescence is detected in the kidneys and ovary of E15.5 heterozygous embryos. Cre recombinase activity is detected in the kidney from E15.5 heterozygous embryos. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are viable and fertile. This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 009389 | B6;129S1-Bambitm1Jian/J | Repository- Live |
| Mice homozygous for this Bambiflox allele are viable and fertile, with loxP sites flanking exon 1 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the endogenous signal peptide deleted in the cre-expressing tissue(s); this is expected to produce a null allele. These mutant mice may be useful in generating conditional mutations for studying the role of Bambi in developmental biology and the TGF-beta pathway. | ||
| 010619 | B6;129S1-Lfngtm1Grid/J | Repository- Live |
| Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. At birth, homozygotes exhibit shortened trunk and tails. Severely affected homozygotes soon die due to respiratory difficulties related to malformed rib cages. Less severely affected homozygotes survive into adulthood. By age embryonic day 8.5, homozygotes exhibit defective somite formation, with indistinct boundaries and irregular shape and size. Vertebral column formation is disrupted, and ribs are bifurcated and fused. Although sclerotome cells condense, the metameric pattern is not maintained. Fusions in the dorsal root ganglia and axonal patterning defects are revealed by histological analysis. | ||
| 010547 | B6;129S1-Notch3tm1Grid/J | Repository- Live |
| Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by Northern blot analysis of brain, lung and heart tissue or Western blot analysis of lung and brain tissue from homozygotes. Homozygotes exhibit disorganized artery wall morphology, and thin vascular smooth muscle cell coat and processes. | ||
| 010544 | B6;129S1-Notch4tm1Grid/J | Repository- Live |
| Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by RT-PCR analysis of lung and kidney tissue from homozygous adult animals, and in situ hybridization of homozygous embryos. Homozygotes exhibit a slightly elevated systolic blood pressure. | ||
| 009388 | B6;129S1-Osr2tm2(cre)Jian/J | Repository- Live |
| Mice homozygous for the Osr2-IresCre (or Osr2IresCre) allele are viable and fertile, with an IRES-Cre bicistronic expression cassette inserted into the 3' UTR of the targeted locus. As such, cre expression is directed by the endogenous promoter/enhancer regions primarily to developing palate mesenchyme and metanephric mesenchyme-derived glomeruli tissues (but not other epithelial and mesenchymal tissues in the developing metanephric kidney). Some ectopic cre activity is reported (particularly in the central nervous system). When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence in these tissues in the offspring. These Osr2-IresCre mice may be useful for generating conditional mutations for studying developmental biology (palate and kidney development). | ||
| 008214 | B6;129S4-Pou5f1tm2Jae/J | Repository- Live |
| Mice homozygous for this Oct4-EGFP mutation are viable and fertile. They harbor an IRES-EGFP fusion cassette downstream of the stop codon of the Oct4 (Pou5f1) gene. When treated with specific transcription factors (Oct4, Sox2, c-Myc and Klf4), some Oct4-EGFP murine embryonic fibroblasts (MEFs) have the properties of induced pluripotent stem (iPS) cells. Such iPS cells have the DNA methylation, gene expression and chromatin state of embryonic stem cells and can form viable chimeras, contribute to the germ line, and generate live late-term embryos when injected into tetraploid blastocysts. These Oct4-EGFP mutant mice may be useful for fluorescent labeling of embryonic stem cells, as well as for the selection of iPS cells (i.e. epigenetic reprogramming of somatic cells into pluripotent embryonic stem cells).
Of note, these Oct4-EGFP mutant mice may also be used in conjunction with Oct4-neo mice (Stock No. 008204); a si ..... | ||
| 009357 | B6;129S6-Adam10tm1Zhu/J | Repository- Live |
| These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissues. When bred to a strain expressing Cre recombinase in T cells for example(see Stock No. 003802), this mutant mouse strain may be useful in studies related to thymocyte developmental defects. | ||
| 007908 | B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | Repository- Live |
| Ai14 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE::deltaNeo conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. Ai14 mice are not expected to express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre is expected to be detectable by fluorescence and mRNA (in situ hybridization). These Ai14 mice are useful as a Cre reporter strain; expressing the red fluorescent protein variant, tdTomato, following Cre-mediated recombination.
The Allen I ..... | ||
| 007905 | B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J | Repository- Live |
| Ai9 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai9 mice do not express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre can be detected by fluorescence and mRNA (in situ hybridization). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-mediated recombination if ..... For more information please see the full phenotype on the strain data sheet | ||
| 012362 | B6;129S6-Tg(Camk2a-cre/ERT2)1Aibs/J | Repository- Live |
| Mice hemizygous for the Camk2a-CreERT2 transgene are viable and fertile, with expression of CreERT2 fusion protein (CreERT2 fusion protein) directed to neural populations by the mouse calcium/calmodulin-dependent protein kinase II alpha promoter region. Cre-ERT2 fusion gene activity is inducible; observed at high levels following tamoxifen administration. When Camk2a-CreERT2 transgenic mice are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in the Camk2a-expressing cells of the double mutant offspring. Specifically, the donating investigator reports that the Camk2a-CreERT2 transgene directs reporter gene expression in sparse populations of neurons in the cortex, hippocampus, striatum, and other structures in the absence of tamoxifen. Following tamoxifen administration, reporter gene expression is turned on in widespread populations of neurons in the same regions ..... For more information please see the full phenotype on the strain data sheet | ||
| 014638 | B6;129X1-Cldn6tm1(cre/ERT2)Dam/J | Repository- Live |
| In this strain, the Cldn6CIHV allele replaces the entire coding region of the claudin 6 (Cldn6) locus with a CreERT2 fusion protein, an internal ribosome entry site (IRES), and a histone H2B-Venus fluorescent protein. This abolishes gene expression. Homozygotes are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. CLDN6 is a structural protein involved in tight junction formation, with a functional role in the epidermal permeability barrier. In this strain endogenous Cldn6 promoter/enhancer regions drive Cre-ERT2 expression and Venus immunofluorescence in embryonic endoderm during organogenesis. Cre-ERT2 fusion gene activity is inducible and only observed following tamoxifen administration. When Cldn6CIHV mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of t ..... For more information please see the full phenotype on the strain data sheet | ||
| 008467 | B6;129X1-Wnt7btm2Amc/J | Repository- Live |
| Mice homozygous for the Wnt7bc3 allele are viable and fertile, with loxP sites flanking exon 3 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). Unlike other Wnt7b mutant alleles, this Wnt7bc3 conditional allele is not affected by alternative exon 1 splicing. These Wnt7bc3 mice may be useful in generating conditional mutations for studying the role of Wnt7b (and other Wnt family members) in development and canonical Wnt signaling cascades, including lung differentiation and growth. In addition, these mice may also be useful in conjunction with other Wnt7 mutant strains including Wnt7b knockout mice (Stock No. 004693) and Wnt7a mutant mice (Stock No. 004715).
When bred to a strain expressing Cre recom ..... | ||
| 009613 | B6;C3-Tg(Scnn1a-cre)3Aibs/J | Repository- Live |
| Hemizygous Scnn1a-Tg3-Cre mice are viable and fertile, with cre expression directed to cortex, thalamus, midbrain, and cerebellum by the Scnn1a promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Scnn1a-Tg3-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex, thalamus, midbrain, and cerebellum).
For characterization information, see images at the Allen Institute for Brain Science website (Scnn1a-Tg3-Cre images). | ||
| 009103 | B6;C3-Tg(Wfs1-cre/ERT2)3Aibs/J | Repository- Live |
| Hemizygous Wfs1-Tg3-CreERT2 mice are viable and fertile. As the Cre-ERT2 fusion gene is under control of the Wfs1 promoter/enhancer regions within the BAC transgene, cre activity is directed to cortex, hippocampus, striatum, thalamus and cerebellum only following tamoxifen administration. The donating investigators may not have assessed expression in tissues other than brain. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone ma ..... For more information please see the full phenotype on the strain data sheet | ||
| 004654 | B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein under the control of the POU domain, class 5, transcription factor 1, promoter and distal enhancer. Primordial germ cell specific markers, alkaline phosphatase II and stage-specific embryonic antigen, are co-expressed in EGFP positive cells. 9.5 and 10.5 dpc (days post-coitum) migratory primordial germ cells from hemizygotes and homozygotes can be sorted and isolated by flow cytometry. This strain represents an effective tool for studying genetic imprinting and early embryonic development. | ||
| 007910 | B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J | Repository- Live |
| These Thy1-Brainbow 1.0 (line L) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Prior to Cre-mediated recombination, the fluorescent protein immediately adjacent to the promoter, dTomato (RFP), is expressed in peripheral and central neurons. When bred to Cre recombinase expressing mice, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): dTomato (RFP) (no recombination), mCerulean (CFP), or mYFP. Integration of tandem transgene copies yields combinatorial fluorescent protein expression in each cell, and thus many possible cell colors, providing a way to distinguish adjacent neurons and visualize other cellular interactions. Of note, the singl ..... For more information please see the full phenotype on the strain data sheet | ||
| 011070 | B6;CBA-Tg(Thy1-EGFP)SJrs/NdivJ | Repository- Live |
| Mice harboring the Thy1-GFP transgene are viable and fertile with enhanced green fluorescent protein (EGFP) expression under the control of a modified Thy1 promoter region (containing the sequences required for neuronal expression but lacking the sequences required for expression in non-neural cells). These thy1-GFP-S mice (derived from founder line S) have EGFP expression in sparse subsets of neurons within specific populations; providing a bright, vital Golgi-like stain. Thy1-GFP-S mice show EGFP labeling in the superficial layers of the neocortex, including most/all interneuron subtypes, with pyramidal/interneuron ratios of approximately 4:1 that match the distribution in the non-labeled population. While this is similar to Thy1-GFPM mice (Stock No. 007788), the labeling distribution for line S is different from line M. In addition, less than 10% of cerebellum mossy fibers show EGFP labeling and no EGFP expressio ..... For more information please see the full phenotype on the strain data sheet | ||
| 013137 | B6;D2-Tg(Akr1b7-RFP)9Amc/J | Repository- Live |
| These transgenic mice express Red Fluorescent Protein (TagRFP-T variant) under the direction of the mouse Akr1b7, aldo-keto reductase family 1, member B7, promoter.
Transgene expression is detected at high levels in the adrenal glands and endothelial cells of renal medullary arterioles of transgenic embryos aged E15.5.
Renal vasculature associated expression is observed in Smooth Muscle Actin immunohistochemical positive cells that are closely associated with FLK1 and PECAM positive intrarenal arteries. Other possible sites of expression have not been characterized.
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous. This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 015853 | B6;DBA-Tg(Cited1-TagRFP)26Amc/J | Repository- Live |
| These transgenic mice express the Tag-RFPT variant of Red Fluorescent Protein under the direction of the mouse Cited1, Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 1, promoter. TagRFP is the optimized monomeric derivative of the tetrameric eqFP578 from the sea anemone, Entacmaea quadricolor.
RFP fluorescence is detected in the cap mesenchyme in the kidney and the male reproductive systems of hemizygous embryos, 15.5 embryonic days of age. Immunohistochemical analysis reveals that the TagRFP-T transgene is expressed in the expected Cited1 expression domain. Other possible sites of expression have not been characterized. Mice that are hemizygous for the knock in allele are viable, fertile, normal in size and do not display physical or behavioral abnormalities.
This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 014160 | B6;DBA-Tg(S100b-EGFP/cre/ERT2)22Amc/J | Repository- Live |
| These transgenic mice express the eGFPCreERT2 (Enhanced Green Fluorescent Protein and cre/ESR1) fusion gene under the direction of the mouse S100b, S100 protein, beta polypeptide, neural, promoter. Transgene expression is detected in chondrocytes in developing bone and in neural cells in the dorsal root ganglia (DRG). GFP fluorescence is not detectable by fluorescent microscope examination of whole embryos, bones or neural tube sagittal slices from embryos aged 15.5dpc. Tamoxifen induced Cre recombinase activity is detected in a subset of the GFP immunoreactive-positive cells in bone and to a lesser extent in the dorsal root ganglia. GFP immunoreactive-positive cells co-localize with S100b immunoreactive-positive cells in bone and DRG. Other possible sites of expression have not been characterized. Mice that are hemizygous for the transgene are viable, fertile, normal in size and do not display physical or behavioral abnormalities. This strain was transfe ..... For more information please see the full phenotype on the strain data sheet | ||
| 014159 | B6;DBA-Tg(Tmem100-EGFP/cre/ERT2)30Amc/J | Repository- Live |
| These transgenic mice express the eGFPCreERT2 (Enhanced Green Fluorescent Protein and cre/ESR1) fusion gene under the direction of the mouse Tmem100, transmembrane protein 100, promoter. Transgene expression is detected in developing nephrons of the kidney, Wolfian and Mullerian ducts and vasculature of the urogenital system of hemizygous 15.5 dpc embryos. GFP fluorescence is detected in embryos 15.5dpc in age in the proximal region of the early renal vesicle, at later stages eGFP is present in the parietal epithelium adjacent to Wilms Tumor (WT1) positive cells demarcating the future podocytes of Bowman's capsule and is limited to the Tmem100 expression domain. Tamoxifen inducible Cre recombinase activity is detected in the developing kidney (nephrogenic zone), vasculature and Wolfian or Mullerian duct of mutant embryos aged 15.5dpc. Possible cre recombinase activity is detected in small renal arteries. Other possible sites of expression have not been char ..... For more information please see the full phenotype on the strain data sheet | ||
| 010803 | B6;FVB-Tg(Adipoq-cre)1Evdr/J | Repository- Live |
| Mice hemizygous for this Adipoq-Cre BAC transgene are viable and fertile, with expression of a Cre recombinase directed to adipose tissue by the promoter/regulatory regions of the mouse adiponectin (Adipoq) locus on the BAC transgene. Transcription/translation from the BAC Adipoq locus is disabled, and Cre recombinase expression levels are similar to that of endogenous Adipoq expression. These mice express Cre recombinase effectively in white adipose tissue (WAT) and brown adipose tissue (BAT), but not in macrophages (including adipose-tissue resident macrophages, alveolar macrophages, or thioglycollate-stimulated peritoneal macrophages). The donating investigator reports highly efficient Cre recombinase activity, with no ectopic expression. The phenotype of homozygous mice was not determined by the donating investigator. These Adipoq-Cre BAC transgenic mice may be useful in generating conditional mutations for studying adipose tissue function and storage, obesity, ..... For more information please see the full phenotype on the strain data sheet | ||
| 012641 | BALB/c-Tg(S100a4-cre)1Egn/YunkJ | Repository- Live |
| These transgenic mice express Cre recombinase under the control of the mouse S100a4, S100 calcium binding protein A4, promoter. Cre recombinase expression is detected specifically in stromal fibroblasts of tissues such as the prostate, forestomach, mammary gland. Mice that are homozygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. | ||
| 008603 | C.129P2(B6)-Gt(ROSA)26Sortm1(tTA)Roos/J | Repository- Live |
| Mice heterozygous for the ROSA:LNL:tTA conditional mutation of the Gt(ROSA)26Sor locus are viable and fertile, with a loxP-flanked STOP cassette preventing transcription of a downstream optimized/modified tetracycline-controlled transactivator protein ("mtTA"). Applying both Cre-lox and Tet-Off technologies, these ROSA:LNL:tTA mutant mice may be useful to generate compound mutant mice in which expression of a tetracycline-responsive promoter element (TRE; also called tet-operator or tetO)-driven gene of interest can be both directed to the cell types defined by the chosen Cre recombinase expression, as well as turned off by the addition of tetracycline (or its analog doxycycline (dox)).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally describe ..... | ||
| 004126 | C.Cg-Cd19tm1(cre)Cgn Ighb/J | Repository- Live |
| The Cd19 promoter specifically directs expression at the earliest stages and throughout B-lymphocyte development and differentiation. A Cre cassette is inserted into the Cd19 exon 2, functionally disrupting the gene. Homozygous mice are Cd19-deficient, whereas heterozygous mice are phenotypically normal and can be used for specific deletion of floxed targets in B-lymphocytes. Mice that are homozygous deficient for Cd19 are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. A deficiency in the B-1 subset of B-lymphocytes is observed along with a concomitant reduction in serum IgM. Homozygous mice are severely impaired in their ability to respond to T-cell-dependent antigens and fail to form splenic germinal centers. | ||
| 010545 | C.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ | Repository- Live |
| These L2G85.BALB/c mice harbor the CAG-luc-eGFP L2G85 transgene. Homozygous mice are viable and fertile, with widespread expression of firefly luciferase and enhanced green fluorescent protein directed by the CAG promoter (human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter). Bioluminesence is detected in heart, spleen, muscle, pancreas, skin, thymus and bone marrow. Luciferase activity is not detected in mature erythrocytes, although low levels are detected in erythrocyte precursors and varying levels of activity in all leukocyte subsets tested. Homozygotes have no reported gross physical or behavioral abnormalities. Following luciferin injection, luciferase expression is generally greater in males than females. GFP fluorescence is detected in skin (upper epidermal layers) by fluorescence microscopy. The Donating Investigator reports that for the FVB-Tg(CAG-luc,-GFP)L2G85Chco/J (STOCK#8450) strain, no GFP fluorescence ..... For more information please see the full phenotype on the strain data sheet | ||
| 007900 | C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J | Repository- Live |
| Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.
For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation. When crossed to a strain expressing Cre recombinase in oocytes (see Stock No. 011062), this mutant mouse strain ma ..... | ||
| 008517 | C57BL/6-Gt(ROSA)26Sortm3(CAG-MIR17-92,-EGFP)Rsky/J | Repository- Live |
| Mice homozygous for the "miR-17-92 transgene" conditional allele are viable and fertile, with a loxP-flanked Neo-STOP cassette preventing transcription of the downstream bicistronic sequences (human miR-17-92 cluster (encoding the precursor of seven miRNA molecules; miR-17-5p, miR-17-3p, miR-18a, miR-19a, miR-20a, miR-19b and miR-92) and EGFP). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of the human miR-17-92 cluster. Because the synthetic CAG promoter driven miR-17-92 transgene was targeted for insertion into the Gt(ROSA)26Sor locus, expression of the transgene is determined by which tissue(s) express Cre recombinase. EGFP fluorescence, however, is not reported following exposure to Cre recombinase (presumably due to RNaseIII excision of the stem-loop structures encoding individual miRNA destabilizing the EGFP portion of the primary transcript ..... For more information please see the full phenotype on the strain data sheet | ||
| 012343 | C57BL/6-Gt(ROSA)26Sortm7(Pik3ca*,EGFP)Rsky/J | Repository- Live |
| Mice homozygous for the R26StopFLP110* conditional allele (also called P110*-transgene) are viable and fertile, with a loxP-flanked Neo-STOP cassette preventing transcription of the downstream bicistronic sequences (P110* [a constitutively active form of the mouse catalytic P110α subunit of phosphatidylinositol 3-kinase] and EGFP). When bred to mice that express Cre recombinase, offspring will have the STOP cassette deleted and subsequent expression of the P110* signal molecule and EGFP fluorescence in the cre-expressing cells. Expression of P110* leads to constitutively active PIK3 heterodimer activity; resulting in the generation of downstream effectors that mediate signal transduction cascades that control cell survival and cell cycle progression (growth, and proliferation). Of note, breeding these mice to an FLP-expressing strain will result in removal of the frt-flanked IRES-EGFP cassette. | ||
| 012352 | C57BL/6-Gt(ROSA)26Sortm8(Map2k1*,EGFP)Rsky/J | Repository- Live |
| Mice homozygous for the R26StopFLMEK1DD conditional allele are viable and fertile, with a loxP-flanked Neo-STOP cassette preventing transcription of the downstream bicistronic sequences (MEK1DD [a mutant form of rat MAPKK1 rendered constitutively active by two serine->aspartic acid substitutions (S218D/S222D) within the catalytic domain] and EGFP). When bred to mice that express Cre recombinase, offspring will have the STOP cassette deleted and subsequent expression of the MEK1DD signal molecule and EGFP fluorescence in the cre-expressing cells. Expression of MEK1DD leads to constitutive activity of MAP Kinase signal transduction pathways that mediate various cellular activities, including gene expression, mitosis, differentiation, proliferation, cell survival, and cell cycle progression. Of note, breeding these mice to an FLP-expressing strain will result in removal of the frt-flanked IRES-EGFP cassette. | ||
| 012361 | C57BL/6-Gt(ROSA)26Sortm9(Rac1*,EGFP)Rsky/J | Repository- Live |
| Mice homozygous for the R26StopFLRACDA conditional allele are viable and fertile, with a loxP-flanked Neo-STOP cassette preventing transcription of the downstream bicistronic sequences (RACDA [RacG12V; a mutant form of Rac1 rendered constitutively active by a glycine->valine substitution at amino acid 12] and EGFP). When bred to mice that express Cre recombinase, offspring will have the STOP cassette deleted and subsequent expression of the RACDA signal molecule and EGFP fluorescence in the cre-expressing cells. Expression of RACDA leads to constitutive activity of Rac1-dependent signal transduction; which is associated with gene expression, proliferation, cell survival, cell cycle progression, cytoskeletal reorganization, and Rho- and CDC42-pathways. Of note, breeding these mice to an FLP-expressing strain will result in removal of the frt-flanked IRES-EGFP cassette. | ||
| 009062 | C57BL/6-Magel2tm1Stw/J | Repository- Live |
| The mouse locus 7qB4/B5 (syntenic with the Prader-Willi region at chromosome position 15q11-q13 in humans) encompasses the cluster of paternally-expressed imprinted genes Magel2, Ndn, Mkrn3, and Peg12. As maternal imprinting silences the Magel2 allele, only the paternally inherited Magel2 allele is expressed. The Magel2-lacZ knock-in allele abolishes endogenous gene function and expresses a β-galactosidase fusion protein. Under control of the upstream promoter/enhancer elements, lacZ expression is directed to the same tissues as the wildtype gene. For example, β-galactosidase expression during embryogenesis is detected in central nervous system (neural tube, forebrain, midbrain and embryonic hypothalamus), peripheral nervous system (dorsal root ganglia and peripheral neurons innervating limb and trunk muscles), and some non-neuronal tissues (genital tubercle, midgut region and placenta). Adult β-galactosidase ..... For more information please see the full phenotype on the strain data sheet | ||
| 016097 | C57BL/6-Tg(Car1-cre)5Flt/J | Repository- Live |
| These transgenic mice express Cre recombinase under the control of the mouse Car1, carbonic anhydrase 1, promoter. Transgenic transcript is detected in the tissues of the large intestine (cecum, proxmial and distal colon) by RT-PCR. Low levels of transcript are detected in the liver, and no transgene transcript is detected in kidney, stomach, bone marrow, prostate, lung, thymus, liver hear, duodenum, jejunum, ileum, pancreas, spleen. Mosaic recombinase activity is detected as early as 14.5 dpc in approximately 15% of the epithelial cells of the large intestine and is observed in individual crypts from the base to lumen. When crossed with a strain containing loxP site-flanked sequences, cre-mediated recombination results in large intestine epithelial-specific deletion of the flanked sequences in the offspring. Mice that are homozygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. | ||
| 003651 | C57BL/6-Tg(Zp3-cre)93Knw/J | Repository- Live |
| This is a transgenic line in which cre expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene. This promoter normally directs expression exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain would be useful for deleting a floxed sequence specifically in the female germ line. The Donating Investigator suggests to accomplish this, females homozygous or heterozygous for the floxed allele, as well as hemizygous for the Zp3cre allele are crossed with wild type males. Progeny will carry the deleted-floxed allele. | ||
| 006481 | C57BL/6J-Tg(ACTB-NOTCH1)1Shn/J | Repository- Live |
| Transgenic mice are viable, fertile and behaviorally normal. These "CALSL-NICD (H)" mice (or simply CALSL-NICD) reportedly carry 10-20 copies of the transgene inserted into a single genomic locus. Expression of the transgene-derived intracellular domain of human NOTCH1 is prevented by a "Lox-STOP-Lox" cassette. When transgenic mice are bred to a strain expressing Cre recombinase, the "floxed stop" cassette is excised in the resulting offspring, and human NOTCH1 expression is observed in the cre-expressing tissue(s). These transgenic mice may be useful in studying early neural progenitor cell development and apoptosis, and responses to tissue-specific Notch activation. For example, when crossed to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771), this transgenic mouse strain may be useful in studies of notch signaling during apoptotic cell death. | ||
| 008661 | C57BL/6J-Tg(Nkx2-1-cre)2Sand/J | Repository- Live |
| Mice homozygous for the Nkx2.1Cre (or BAC-Nkx2.1-Cre) transgene are viable and fertile, with cre expression directed to major subgroups of brain interneuron progenitors, developing lung, thyroid, and pituitary by the Nkx2.1 promoter/enhancer regions within the BAC transgene. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. For example, when bred with beta-galactosidase reporter mice, the first detectable Cre recombinase expression in double mutant offspring is observed on embryonic day (E)10.5 in the basal telencephalon. These Nkx2.1Cre (or BAC-Nkx2.1-Cre) transgenic mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain progenitors (including future major interneurons in telencephalon and hypothalamus cells), as well as developing lung, thyroid, and pituitary. | ||
| 004597 | C;129S4-Ptentm1Hwu/J | Repository- Live |
| These mice possess loxP sites flanking exon 5 of the targeted gene. Mice homozygous for the "floxed" allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.
For example, when crossed to a strain expressing Cre recombinase in astrocytes (see Stock No. 012887), this mutant mouse strain may be useful in studies of neurogenesis. When crossed to a strain expressing Cre recombinase in the central nervous system (see Stock No. 004600), this mutant mouse strain may be useful in studies of glia differentiation and cerebellar development. | ||
| 008040 | CBy.B6-Gt(ROSA)26Sortm1(HBEGF)Awai/J | Repository- Live |
| Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.
For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation. When crossed to a strain expressing Cre recombinase in the pituitary and, at lower levels, in the testes (see Stock No. > ..... | ||
| 008450 | FVB-Tg(CAG-luc,-GFP)L2G85Chco/J | Repository- Live |
| Mice homozygous for the CAG-luc-eGFP L2G85 transgene are viable and fertile, with widespread expression of firefly luciferase and enhanced green fluorescence protein directed by the CAG promoter (human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter). Bioluminesence is detected in heart, spleen, muscle, pancreas, skin, thymus and bone marrow. Luciferase activity is not detected in mature erythrocytes, although low levels are detected in erythrocyte precursors and varying levels of activity in all leukocyte subsets tested. Homozygotes have no reported gross physical or behavioral abnormalities. Following luciferin injection, luciferase expression is generally greater in males than females. GFP fluorescence is detected in skin (upper epidermal layers) by fluorescence microscopy. The Donating Investigator reports that for the FVB-Tg(CAG-luc,-GFP)L2G85Chco/J (STOCK#8450) strain, no GFP fluorescence is detected in hematopoiet ..... For more information please see the full phenotype on the strain data sheet | ||
| 006774 | FVB-Tg(Col2a1-cre/ERT)KA3Smac/J | Repository- Live |
| Mice hemizygous or homozygous for the Col2CreERT transgene are viable and fertile. Mice from this founder line (line K from founder mouse A3) have strong tamoxifen-inducible cre expression directed to cells of the chondrogenic lineage (cartilage), with minimal (<0.1%) cre activity in the absence of tamoxifen. The CreERT protein consists of Cre recombinase fused to a mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligand 4-hydroxytamoxifen. Restricted to the cytoplasm, CreERT can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Col2CreERT mice are bred with mice containing a loxP-flanked ..... For more information please see the full phenotype on the strain data sheet | ||
| 006954 | FVB-Tg(Ddx4-cre)1Dcas/J | Repository- Live |
| Mice hemizygous for this Vasa-Cre transgene are viable and fertile. Transgenic cre activity is directed to male and female germ cells starting at embryonic day (e)15-e18. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequence. In such breedings, occasional hemizygous mice may exhibit variegated cre expression in skin epithelium or global cre expression (<20% incidence). Differential parent-of-origin transgene expression is observed. When the mother harbors Vasa-Cre, virtually all progeny undergo global Cre-mediated recombination, even those that do not inherit the transgene (which may be useful in converting a "floxed" allele to a null while obviating the need to perform additional crosses to remove the transgene). To achieve germ-line specific Cre-mediated recombination in offspring, paternal Vasa-Cre mice should be used.
In crosses with some floxed alleles, gl ..... | ||
| 005125 | FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J | Repository- Live |
| These mice contain the firefly luciferase (luc) gene inserted into the Gt(ROSA)26Sor locus. Expression of the luciferase gene is blocked by a loxP-flanked STOP fragment placed between the luc sequence and the Gt(ROSA)26Sor promoter. This strain serves as a Cre reporter strain. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision is indicated by luciferase expression in Cre-expressing tissues. Mice that are heterozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. | ||
| 014140 | FVB.Cg-Myod1tm2.1(icre)Glh/J | Repository- Live |
| The MyoDiCre targeting vector was designed to replace exon 1 of the Myogenic differentiation 1 (Myod1) gene with a modified Cre-recombinase, iCre, abolishing Myod1 gene function. This optimized variant of Cre recombinase, driven by Myod1 promoter/enhancer elements, improves translational efficiency and reduces epigenic silencing. Heterozygous mice are viable, fertile, and normal in size, while homozygous mice exhibit reduced fitness and survival prior to weaning. Myod1 is a muscle regulatory factor and a marker of commited myogenic cells. When MyoDiCre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Myod1-expressing cells of the offspring. These mice may be useful for lineage analysis and skeletal muscle-specific gene ablation. | ||
| 003516 | FVB.Cg-Tg(CAG-EGFP)B5Nagy/J | Repository- Live |
| This transgenic strain expresses an Enhanced Green Fluorescent Protein (Clontech) driven by chicken beta-actin promoter and CMV intermediate early enhancer. Mice, and cells derived from them, are distinguished from wildtype on the basis of fluorescence. The transgene is expressed in all nucleated embryonic tissues. Cells and tissues with increased hemoglobin content exhibit reduced fluorescence as development progresses. In newborn and adult mice, the entire organ system expresses EGFP. Though widespread, expression levels vary between different organs. This strain can be used as a source of fluorescently marked cells or tissues. Animals from stock 003516 homozygous for Tg(CAG-EGFP)B5Nagy allele have displayed an increased incidence of lymphoma compared to animals hemizygous for this allele. Lymphoma in homozygous breeders for stock 003516 has been observed as early as 4 months of age. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles ..... | ||
| 002856 | FVB/N-Tg(TIE2-lacZ)182Sato/J | Repository- Live |
| Transgenic mice carry a beta-galactosidase reporter gene under the control of the murine Tek (Tie2) promoter. LacZ is expressed specifically in vascular endothelial cells in embryonic and adult mice. The transgenic line may be useful when crossed with tumor producing strains and the transgene used to visualize neovascularization during tumorigenesis. | ||
| 010542 | NOD.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ | Repository- Live |
| These L2G85.NOD mice harbor the CAG-luc-eGFP L2G85 transgene. Mice homozygous for the CAG-luc-eGFP L2G85 transgene are viable and fertile, with widespread expression of firefly luciferase and enhanced green fluorescence protein directed by the CAG promoter (human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter). Bioluminesence is detected in heart, spleen, muscle, pancreas, skin, thymus and bone marrow. Luciferase activity is not detected in mature erythrocytes, although low levels are detected in erythrocyte precursors and varying levels of activity in all leukocyte subsets tested. Homozygotes have no reported gross physical or behavioral abnormalities. Following luciferin injection, luciferase expression is generally greater in males than females. GFP fluorescence is detected in skin (upper epidermal layers) by fluorescence microscopy. The Donating Investigator reports that for the FVB-Tg(CAG-luc,-GFP)L2G85Chco/J (STOC ..... For more information please see the full phenotype on the strain data sheet | ||
| 009597 | STOCK Adam17tm1.2Bbl/J | Repository- Live |
| Mice homozygous for this Taceflox allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed sequences deleted in the cre-expressing tissue producing a null allele. As the proinflammatory cytokine TNF-α and other other cell receptors are synthesized as membrane-bound precursors that need to be proteolytically released by functional TNF-α converting enzyme (TACE or Adam17 [a disintegrin and metallopeptidase domain 17]), these Taceflox mutant mice may be useful in generating conditional mutations for studying TNF-sheddase function, TNF-related autoimmune diseases. | ||
| 007569 | STOCK Fgfr2tm1Dor/J | Repository- Live |
| Mice homozygous for this Fgfr2flox allele possess loxP sites flanking exons 8-10 of the targeted gene and are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have sequences encoding the alternatively spliced Ig domain IIIb, as well as the IIIc and TM domains, deleted in the cre-expressing tissue(s). These Fgfr2-flox mutant mice may be useful in generating conditional mutations to study the role of fibroblast growth factor receptors in vertebrate development; including early embryogenesis, regional specification of the brain, limb morphogenesis, and normal bone, craniofacial, and lens development.
For example, when crossed to a strain expressing Cre recombinase in the central nervous system, especially astrocytes (see Stock No. 004600), this mutant mouse strain may be useful in studies of astroglial migration. When crossed to ..... | ||
| 008464 | STOCK Foxa2tm2.1(cre/Esr1*)Moon/J | Repository- Live |
| This strain expresses the tamoxifen-inducible cre/Esr1 from the targeted locus. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions specifically in the developing endoderm, notochord, and floorplate. Heterozygotes and homozygotes are normal in size, viability and fertility. | ||
| 008194 | STOCK Gata4tm1.1Sad/J | Repository- Live |
| Mice homozygous for this Gata4loxP conditional allele are viable and fertile, with loxP sites flanking exons 3-5 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for both zinc finger DNA-binding domains and the nuclear localization signal essential for GATA4 function) deleted in the cre-expressing tissue(s). These GATA binding protein 4 conditional mice may be useful in generating conditional mutations for studying GATA4 function during organogenesis (such as cardiogenesis) or in adult mice.
For example, when crossed to a strain expressing Cre recombinase in cardiac myocytes (see Stock No. 009074), this mutant mouse strain may be useful in studies of cardiac hypertrophy, stress-compensation and myocyte viability. When bred to a strain expressing Cre recombinase in heart muscle (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 007913 | STOCK Gli1tm3(cre/ERT2)Alj/J | Repository- Live |
| Mice homozygous for this Gli1-CreERT2 targeted allele are viable and fertile (although homozygous males are reported to have breeding problems). Under control of the endogenous upstream promoter/enhancer elements, tamoxifen-inducible cre activity is observed in cells that have received positive Hedgehog/Sonic Hedgehog signaling. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Gli1-CreERT2 mice are ..... For more information please see the full phenotype on the strain data sheet | ||
| 007922 | STOCK Gli2tm2.1Alj/J | Repository- Live |
| Mice homozygous for this Gli2lzki allele harbor a β-galactosidase "knock-in" (lzki) allele that also abolishes endogenous gene function. As such, homozygous mice die at birth with many defects including absence of Sonic Hedgehog-expressing floor plate cells, reduction of Nkx2.2-expressing V3 interneurons in the spinal cord, and defects in midbrain, cerebellum and lung development. Under control of the upstream promoter/enhancer elements, lacZ expression is observed in a pattern identical to the wild-type gene. Heterozygotes are viable and fertile. These Gli2lzki mice may be useful for studying Hedgehog/Sonic Hedgehog signaling in the development of many organs (such as central nervous system and axis patterning), as well as the role of Gli2 in adult organs. | ||
| 007926 | STOCK Gli2tm6Alj/J | Repository- Live |
| Mice homozygous for this Gli2flox conditional allele are viable and fertile, with loxP sites flanking exons 7 and 8 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have these exons deleted in the cre-expressing tissue(s). This results in a frameshift mutation following splicing of mRNA from exon 6 to 9 and is reported to confer the null phenotype. These Gli2flox mutant mice may be useful in generating conditional mutations for studying Hedgehog/Sonic Hedgehog signaling in the development of many organs (such as central nervous system and axis patterning), as well as the role of Gli2 in adult organs. | ||
| 008873 | STOCK Gli3tm1Alj/J | Repository- Live |
| Mice homozygous for this Gli3flox conditional allele are viable and fertile, with loxP sites flanking exon 8 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have exon 8 deleted in the cre-expressing tissue(s). This results in a frameshift mutation upstream of the DNA-binding domain following splicing of mRNA from exon 7 to 9 and is reported to confer the null phenotype. These Gli3flox mutant mice may be useful in generating conditional mutations for studying Hedgehog/Sonic Hedgehog signaling in the development of many organs (such as central nervous system and limb patterning), as well as the role of Gli3 in adult organs.
When bred to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771 for example), this mutant mouse strain may be useful in studies of mid/hind brain development. When bred to a str ..... | ||
| 013731 | STOCK Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/J | Repository- Live |
| Mice homozygous for the R26R-Confetti conditional allele are viable and fertile, with a CAG promoter, loxP site, and STOP cassette preventing transcription of the downstream Brainbow 2.1 sequences. The Brainbow 2.1 region contains two loxP-flanked dimers, each uniquely positioned in head-to-tail tandem. One dimer has nuclear-localized green fluorescent protein (hrGFPII) and a reverse-oriented cytoplasmic yellow fluorescent protein (mYFP). The other dimer has cytoplasmic red fluorescent protein (tdimer2(12)) and a reverse-oriented membrane-tethered cyan fluorescent protein (mCerulean). The Brainbow2.1 region may be written as loxP-STOP-loxP-GFP-PFY-Pxol-loxP-RFP-PFC-Pxol to show the transcriptional direction of each part. When bred to mice that express Cre recombinase, the resulting offspring may have a recombination event that stochastically places one of the four fluorescent proteins into position directly downstream of the CAG promote ..... For more information please see the full phenotype on the strain data sheet | ||
| 006331 | STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J | Repository- Live |
| Homozygous "ROSA26-eGFP-DTA" mice are viable, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator reports that some homozygous males are subfertile. Mutant mice display widespread expression of EGFP, but DTA transcription is prevented by a strong transcriptional stop sequence. When bred to mice that express Cre recombinase under the control of a promoter of interest, the loxP-flanked EGFP and stop sequence are removed, and DTA expression is activated, resulting in the specific ablation of cre-expressing cells. This strain may be useful on its own as a fluorescent reporter or in combination with cre-expressing mice to produce conditional deletions of specific groups of cells. Transgenic mice also may have applications in toxicology and protein synthesis research.
For example, when crossed to a strain expressing Cre recombinase in the midbrain and dorsal spinal cord (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 008159 | STOCK Gt(ROSA)26Sortm1(Notch1)Dam/J | Repository- Live |
| These mice contain a sequence encoding an intracellular portion of the mouse Notch1 gene (amino acids 1749-2293), but lacking the c-terminal PEST domain, and Green Fluorescent Protein, GFP, inserted into the GT(ROSA)26Sor locus. Expression of the Notch1 fragment and GFP is blocked by a loxP-flanked STOP fragment placed between the coding sequence and the GT(ROSA)26Sor promoter. The GFP expression is localized to the nucleus by an IRES sequence. The truncated cytoplasmic fragment encoded by the Notch1 sequence causes constitutive signaling activity. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants for studying the effects of Notch pathway activation. Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
For example, when crossed to a strain expressing a tamoxifen inducible Cre recombinase in all c ..... | ||
| 005130 | STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J | Repository- Live |
| These mice contain an Enhanced Yellow Fluorescent Protein/Smoothened homolog (Drosophila) fusion gene inserted into the Gt(ROSA)26Sor locus. The mutant allele consists of a fusion product involving Enhanced Yellow Fluorescent Protein (EYFP) and the constitutively active W539L point mutation of the mouse smoothened homolog (Drosophila) gene (SmoM2). Expression of the Smo/EYFP fusion gene is blocked by a loxP-flanked STOP fragment placed between the Gt(ROSA)26Sor promoter and the Smo/EYFP sequence. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision results in the constitutive expression of mouse smoothened homolog (Drosophila) and unrestrained Hedgehog signaling in Cre-expressing tissues. Expression of the SmoM2 fusion protein can be monitored using EYFP-specific fluorescence protocols. Mice that are homozygous for the mutant allele are viable, fertile, normal in size and do not display any gross physical ..... For more information please see the full phenotype on the strain data sheet | ||
| 011011 | STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J | Repository- Live |
| Mice homozygous for both targeted mutations (R26-rtTA and Col1a1::2lox-tetO-4F2A) are viable and fertile. These double mutant R26rtTA;Col1a12lox-4F2A mice have widespread expression of the optimized form of reverse tetracycline-controlled transactivator (rtTA-M2) protein directed to multiple tissues by the Gt(ROSA)26Sor promoter. In the absence of the tetracycline analog doxycycline (dox), expression of the loxP-flanked, dox-inducible 4F2A cassette from the Col1a1 locus is not detected. Following dox administration, this single-gene transgenic mouse strain expresses the polycistronic 4F2A cassette (four mouse reprogramming genes Oct4 [Pou5f1], Sox2, Klf4, and c-Myc [Myc]). Somatic expression of these reprogramming factors allows multiple somatic cell types to be directly reprogrammed to generate induced pluripotent stem cells (iPSCs) by culture in dox (see details below). Because the 4F2A reprogramming factors are f ..... For more information please see the full phenotype on the strain data sheet | ||
| 008600 | STOCK Gt(ROSA)26Sortm1(tTA)Roos/J | Repository- Live |
| Mice heterozygous for the ROSA:LNL:tTA conditional mutation of the Gt(ROSA)26Sor locus are viable and fertile, with a loxP-flanked STOP cassette preventing transcription of a downstream optimized/modified tetracycline-controlled transactivator protein ("mtTA"). Applying both Cre-lox and Tet-Off technologies, these ROSA:LNL:tTA mutant mice may be useful to generate compound mutant mice in which expression of a tetracycline-responsive promoter element (TRE; also called tet-operator or tetO)-driven gene of interest can be both directed to the cell types defined by the chosen Cre recombinase expression, as well as turned off by the addition of tetracycline (or its analog doxycycline (dox)). | ||
| 012266 | STOCK Gt(ROSA)26Sortm5(ACTB-tTA)Luo/J | Repository- Live |
| Mice homozygous for this ROSA26-ZtTA (or ZtTA) conditional allele are viable and fertile, although the donating investigator reports that heterozygous mice are more healthy and fertile than homozygous mice. This ROSA26-ZtTA (or ZtTA) conditional allele is designed with a loxP-flanked β-geo transcriptional STOP cassette preventing transcription of the downstream tetracycline-controlled transactivator protein (tTA). When bred to mice that express Cre recombinase, offspring will have the STOP cassette deleted and subsequent expression of the tTA in the cre-expressing cells. The donating investigator reports that the CMV enhancer/chicken beta-actin core promoter (pCA) allows stronger and persistent expression of the downstream tTA (especially in adult cells) compared to the endogenous Gt(ROSA)26Sor locus alone. Applying both Cre-lox and Tet-Off technologies, these ZtTA mutant mice may be useful to generate compound mutant mice in which expression of a tetracyclin ..... For more information please see the full phenotype on the strain data sheet | ||
| 008458 | STOCK Mirc1tm1.1Tyj/J | Repository- Live |
| The miR-17~92 (Mir17, Mir18, Mir19a, Mir20a, Mir19b-1, Mir92-1) cluster, overexpressed in human cancers, is flanked by loxP sites in this targeted mutation strain. Mice homozygous for the floxed miR17~92 allele (miR17~92fl/fl) are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. | ||
| 012871 | STOCK Pik3r1tm1Lca/J | Repository- Live |
| Mice homozygous for this p85αloxP allele are viable and fertile, with loxP sites flanking exon 7 of the targeted gene. The Pik3r1 locus encodes three proteins (p85α, p55α, and p50α) that arise from alternative transcription initiation sites; and exon 7 is the first common exon for all three isoforms. When bred to mice that express Cre recombinase, the resulting offspring will have exon 7 deleted in the cre-expressing tissue(s); splicing of upstream exons (exon 6, 1b, or 1c) directly into the downstream exon 8 results in a frameshift mutation that introduces an immediate stop codon. Such a deletion should prevent the translation of the SH2 and p110-binding domains, eliminating the ability to form a functional protein from any of the three transcription initiation sites. These mutant mice may be useful in generating conditional mutations for studying class IA phosphoinositide 3-kinases (PI3Ks) in cell growth, cell proliferation cell ..... For more information please see the full phenotype on the strain data sheet | ||
| 012620 | STOCK Trp53tm1Brd Brca1tm1Aash Tg(LGB-cre)74Acl/J | Repository- Live |
| BLG-Cre; Brca1F22-24/F22-24; p53+/- mice carry the beta-lactoglobulin (BLG)-Cre transgene, are homozygous for floxed exons 22-24 of the breast cancer 1 (Brca1) allele, and are heterozygous for p53 tumor-suppressor gene (p53) deficiency. Mice of this genotype are viable, fertile, normal in size and do not display any behavioral abnormalities. BLG-Cre; Brca1F22-24/F22-24; p53+/- females have expression of the BLG-Cre transgene during lactation; which leads to loss of Brca1 function in the mammary gland. This results in formation of mammary tumors exhibiting high grade central necrosis and metaplastic elements in the form of spindle cell and squamous cell differentiation; as seen in human basal-like breast cancers and BRCA1 mutation carriers. Heterozygosity for the mutant p53 allele accelerates the formation of mammary tumors. This strain may be useful for studying human basal-like cancer and breast ..... For more information please see the full phenotype on the strain data sheet | ||
| 010911 | STOCK Wt1tm1(EGFP/cre)Wtp/J | Repository- Live |
| Homozygous mice die between embryonic day (E)13.5 and birth with defects of heart, kidney, gonads, and multiple other organs. Heterozygous (Wt1GFPCre/+) mice are viable and fertile. The Wt1GFPCre "knock-in" allele both abolishes Wt1 gene function and expresses an enhanced green fluorescent protein-Cre recombinase fusion protein (EGFPCre) from the Wt1 promoter/enhancer elements. In heart from heterozygous mice, EGFPCre expression is directed to proepicardium and epicardium from E9.5 to E15.5, and is not found in the myocardium. When bred to mice containing loxP-flanked sequences, the resulting offspring will have Cre-mediated deletion of the floxed sequences in the Wt1-expressing cells (and their descendants). As Wt1 is expressed in the developing genitourinary system and in the mesothelia overlying most visceral organs, these mutant mice may be useful as fluorescent/Cre-lox tools for lineage-tracing/marking Wt1-expressin ..... For more information please see the full phenotype on the strain data sheet | ||
| 010912 | STOCK Wt1tm2(cre/ERT2)Wtp/J | Repository- Live |
| Homozygous mice die between embryonic day (E)13.5 and birth with defects of heart, kidney, gonads, and multiple other organs. Heterozygous (Wt1CreERT2/+) mice are viable and fertile. The Wt1CreERT2 "knock-in" allele both abolishes Wt1 gene function and has expression of the CreERT2 fusion protein (CreERT2) under control of the Wt1 promoter/enhancer elements. In heart from heterozygous mice, CreERT2 expression is directed to proepicardium and epicardium from E9.5 to E15.5, and is not found in the myocardium. CreERT2 fusion gene activity is inducible; observed following tamoxifen administration. As such, when Wt1CreERT2 mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in the Wt1-expressing cells of the offspring. The donating investigator reports that Cre activity may be observed prior to tamoxifen exposure only in ..... For more information please see the full phenotype on the strain data sheet | ||
| 013749 | STOCK Tg(ACTB-EGFP,-tdTomato)11Luo/J | Repository- Live |
| Homozygous MADM-11GT mice are viable and fertile with no gross behavioral or observable abnormalities. The MADM-11GT allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP), a beta-globin intronic sequence containing an frt site and a loxP-flanked neomycin resistance gene, and the MYC-tagged C-terminal portion of a red fluorescent protein (tdTomato) all inserted into the Hipp11 locus on chromosome 11 (cytoband A1 at ~3cM between the Eif4enif1 and Drg1 loci). These MADM-11GT mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to MADM-11TG mice harboring a reciprocal mutation at the same locus (see Stock No. 013751). The resulting GT/TG offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygou ..... For more information please see the full phenotype on the strain data sheet | ||
| 014092 | STOCK Tg(ACTB-tTA2,-MAPT/lacZ)1Luo/J | Repository- Live |
| Homozygous CAG-stop-tTA2 transgenic mice are viable and fertile. CAG-stop-tTA2 transgenic mice harbor the ii-CAG-stop-tTA2-IRES-tau-lacZ-ii transgene; designed with a loxP-flanked transcriptional STOP cassette preventing transcription of the downstream modified tetracycline-regulated transactivator (tTA2). The ii-CAG-stop-tTA2-IRES-tau-lacZ-ii transgene is flanked by two copies of the chicken β-globin HS4 insulator on each side to preserve expression fidelity (see additional information below). When bred to mice that express a tamoxifen-inducible Cre recombinase (CreERT2), administration of tamoxifen to the double mutant offspring allows the CreERT2 fusion protein to enter the nucleus of the cre-expressing cells; this deletes the STOP cassette and results in expression of tTA2. The donating investigator reports that tau-lacZ fusion protein expression in the tamoxifen-treated double mutant offspring is faint. Of note, the donating investigator h ..... For more information please see the full phenotype on the strain data sheet | ||
| 013751 | STOCK Tg(ACTB-tdTomato,-EGFP)11Luo/J | Repository- Live |
| Homozygous MADM-11TG mice are viable and fertile with no gross behavioral or observable abnormalities. The MADM-11TG allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of a red fluorescent protein (tdTomato), a beta-globin intronic sequence containing an frt site and a loxP-flanked neomycin resistance gene, and the C-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP) all inserted into the Hipp11 locus on chromosome 11 (cytoband A1 at ~3cM between the Eif4enif1 and Drg1 loci). These MADM-11TG mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to MADM-11GT mice harboring a reciprocal mutation at the same locus (see Stock No. 013749). The resulting TG/GT offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous"), and mu ..... For more information please see the full phenotype on the strain data sheet | ||
| 014177 | STOCK Tg(Afp-mCherry)1Hadj/J | Repository- Live |
| Afp::mCherry transgenic mice have the alpha fetoprotein (Afp) promoter/enhancer sequences driving expression of a monomeric red fluorescent protein, mCherry. Hemizygotes are viable, fertile, and normal in size. Under control of Afp, mCherry labels the visceral endoderm and its derivatives, including the visceral yolk sac and gut endoderm. Expression is also seen in fetal liver and pancreas, and in liver, pancreas, digestive tract and brain of postnatal mice. These mice may be useful as a bright and photostable means for visualizing morphogenesis and tissue rearrangements in the developing embryo. | ||
| 007684 | STOCK Tg(Atoh1-cre/Esr1*)14Fsh/J | Repository- Live |
| Mice hemizygous for this Math1-CreERT2 transgene are viable and fertile. Under control of the Math1 (Atoh1) enhancer, tamoxifen-inducible cre activity is observed in neural progenitors of the cerebellar rhombic lip, dorsal hindbrain and spinal cord, as well as in inner-ear primordia (with a limited amount of ectopic expression in the primordium of the hippocampus but not the cortex). The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone ma ..... For more information please see the full phenotype on the strain data sheet | ||
| 005441 | STOCK Tg(CAG-DsRed*MST)1Nagy/J | Repository- Live |
| Mice homozygous for this Actb-DsRed.T3 transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the red fluorescent protein variant DsRed.MST under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. All tissues of homozygotes fluoresce red. Mice hemizygous for the transgene express red fluorescent protein less intensely than homozygotes. Expression is observed throughout all embryonic and adult stages and very high expression is found in pancreas, skeletal muscle, heart and seminal vesicle. | ||
| 003116 | STOCK Tg(CAG-EGFP)D4Nagy/J | Repository- Live |
| This transgenic strain carries the Enhanced Green Fluorescent Protein (Clontech) driven by chicken beta-actin promoter and CMV intermediate early enhancer. The transgene was found to have integrated on the X chromosome. Mice of this strain, and cells derived from them, can be distinguished from wildtype on the basis of fluorescence. Transgene expression is seen as early as ~embryonic day 2.75, as morula stage embryos begin to compact. Expression of EGFP is spatiotemporally widespread, though levels differ between different lineages (e.g., expression is slightly higher in the heart). As the transgene is located on the X chromosome, transgenic males will exclusively transmit the EGFP transgene to their female offspring, providing the basis for a non-invasive sexing assay based on green fluorescence. These mice may be useful for sex discrimination prior to overt sexual dimorphism (embryonic day 12.5), monitoring X-inactivation, and may be a source of fluorescently marked cells/tiss ..... For more information please see the full phenotype on the strain data sheet | ||
| 011106 | STOCK Tg(CAG-GFP*)1Hadj/J | Repository- Live |
| Mice harboring the lipid modified CAG-GFP* transgene are viable and fertile, normal in size and do not display any gross physical or behavioral abnormalities. The CAG promoter directs widespread expression of a glycosylphosphatidylinositol-tagged (GPI) green fluorescent protein (GFP) fusion throughout embryonic development and adulthood. Expression is targeted to the plasma membrane, Golgi membranes and some secretory vesicles. Expression is enriched in the apical regions of the plasma membrane. Hemizygotes and homozygotes exhibit identical distribution patterns, however, homozygotes exhibit increased fluorescence. This mutant mouse strain may be useful for in vivo imaging of cell morphology, plasma membrane dynamics and lipid localization. | ||
| 013753 | STOCK Tg(CAG-KikGR)33Hadj/J | Repository- Live |
| CAG::KikGR33 transgenic mice express a Kikume Green-Red (KikGR) photoconvertible fluorescent protein under the control of a CMV enhancer/chicken beta-actin promoter (CAGGS) promoter. Mice homozygous for the transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. KikGR, engineered from Favia favus coral, changes color from green to red upon activation in embryos, adult mice, and embryonic stem (ES) cells. At basal state, green fluorescence is seen in all cells. A single cell or group of cells at basal state, exposed to 405 nm wavelength light, undergo photo conversion and fluoresce red. Since KikGR is developmentally neutral and non-toxic, the movement of these fluorescent cells, and their progeny, can be imaged during embryonic development. Mice from founder line 33 exhibits widespread expression of KikGR, while mice from founder line 75 (Stock No. ..... For more information please see the full phenotype on the strain data sheet | ||
| 009615 | STOCK Tg(Cartpt-cre)1Aibs/J | Repository- Live |
| Hemizygous Cart-Tg1-Cre mice are viable and fertile, with cre expression directed to cortex, hippocampus, and cerebellum by the Cartpt promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Cart-Tg1-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex, hippocampus, and cerebellum).
For characterization information, see images at the Allen Institute for Brain Science website (Cart-Tg1-Cre images). | ||
| 008241 | STOCK Tg(Cspg4-DsRed.T1)1Akik/J | Repository- Live |
| Mice hemizygous for the NG2DsRedBAC transgene are viable and fertile, expressing an optimized red fluorescent protein variant (DsRed.T1) under the control of the mouse NG2 (Cspg4) promoter/enhancer. DsRed.T1 fluorescence is detected in NG2 cells (oligodendrocyte progenitor cells) throughout the postnatal central nervous system, including the gray and white matter of the brain, cerebellum and spinal cord, and vascular mural cells; but not in mature oligodendrocytes, astrocytes, resting microglia, or neurons. DsRed.T1 fluorescence intensity is highest in cell bodies and can also be detected in distal processes, suggesting that tetrameric DsRed.T1 remains soluble and is not toxic to cells. In addition, DsRed.T1 fluorescence may be readily detected without using anti-DsRed antibodies and is suitable for identifying NG2 cells in live slices or for purifying NG2 cells via FACS. These NG2DsRedBAC transgenic mice may be useful for fluorescent labeling of NG2 cells (oligodendrocyte p ..... For more information please see the full phenotype on the strain data sheet | ||
| 004623 | STOCK Tg(Fos-lacZ)34Efu/J | Repository- Live |
| These TOPGAL transgenic mice are a reporter strain that express Beta-galactosidase in the presence of the lymphoid enhancer binding factor 1/transcription factor 3 (LEF/TCF) mediated signaling pathway and activated Beta-catenin. The transgene contains the lacZ gene under the control of a regulatory sequence consisting of three consensus LEF/TCF-binding motifs upstream of a minimal c-fos promoter. Transgenic mice display TOPGAL activity (Beta-galactosidase activity) during early embryonic development in a subset of pluripotent embryonic basal cells of the epithelium and dermis of developing hair follicles, but not during the next stage of hair follicle development; formation of hair germs. TOPGAL transgene activity reappears in hair follicles at E16.5 and TOPGAL expression is strongly upregulated in the postnatal hair shaft precursor cells in both whisker and body hair anagen follicles (active periods of hair growth). TOPGAL expression ceases during catagen (regression and ..... For more information please see the full phenotype on the strain data sheet | ||
| 011062 | STOCK Tg(Gdf9-cre)5092Coo/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse growth differentiation factor 9 (Gdf9) promoter. Cre recombinase expression is detected in oocytes of the primordial follicles by postnatal day 3 and in oocytes, but not somatic cells, of all follicles at the primary, secondary and later stages by 24 days. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in the offspring. This mutant mouse strain may be useful in studies of studies of folliculogenesis and oocyte development. | ||
| 014600 | STOCK Tg(I12b-cre/ERT2,-ALPP)37Fsh/J | Repository- Live |
| These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse Dlx1/2, distal-less homeobox 1, distal-less homeobox 2, forebrain enhancer/ promoter (I12b). The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain, and the human placental alkaline phosphatase (ALPP or PLAP). The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/ESR1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing loxP site flanked sequences, the flanked sequences will be deleted in the cre-expressing tissues in the offspring upon administration of tamoxifen. Tamoxifen administration induces Cre recombination in the ventral telencephalon and diencephalon as early as em ..... For more information please see the full phenotype on the strain data sheet | ||
| 005650 | STOCK Tg(Myh6-cre/Esr1*)1Jmk/J | Repository- Live |
| The alpha-MHC-MerCreMer transgene has the mouse Myh6 promoter (myosin, heavy polypeptide 6, cardiac muscle, alpha; alpha-MHC) directing expression of a tamoxifen-inducible Cre recombinase (MerCreMer) to juvenile and adult cardiac myocytes. Mice homozygous for the alpha-MHC-MerCreMer transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre recombinase expression in heart tissue is confirmed by western blot. Southern blot confirmed heart cell specificity compared to brain, kidney, lung, liver, and skeletal muscle. Insertion of this transgene and its protein show no changes in echocardiography, heart mass or pathology, or hypertrophy marker genes compared to nontransgenic littermates. Of note, the MerCreMer double fusion protein has substantially greater Cre recombinase activity with less promiscuity compared with the CreMer single fusion protein. When alpha-MHC-MerCreMer transgenic mice are bred with mice containing For more information please see the full phenotype on the strain data sheet | ||
| 014158 | STOCK Tg(Pax4-cre)1Dam/J | Repository- Live |
| Pax4-cre transgenic mice have the mouse paired box gene 4 (Pax4) promoter directing expression of an enhanced green fluorescent protein fused to a Tet-off cassette (EGFP/tTA). The transgene also contains a tetracycline-responsive element with a CMV minimal promoter (tetO-CMVmin) driving expression of a Cre-recombinase gene. In the absence of tetracycline/doxycycline, Cre recombinase expression is observed in Pax4-expressing cells (pancreatic progenitor cells). While designed to concomitantly allow tetracycline/doxycycline-dependant inhibition of Cre recombinase expression, the donating investigator confirms no such inhibition is observed. Also, no GFP expression is observed with or without tetracycline/doxycycline administration. Therefore, Pax4-cre transgenic mice function only for Cre recombinase expression in Pax4-expressing cells. When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of ..... For more information please see the full phenotype on the strain data sheet | ||
| 008477 | STOCK Tg(RARE-Hspa1b/lacZ)12Jrt/J | Repository- Live |
| These transgenic mice express beta-galactosidase (lacZ) gene under the control of the retinoic acid responsive element (RARE). Sporadic beta-galactosidase activity is detected in less than half of embryonic day 3.5 blastocysts. Beta-galactosidase activity in embryos 11.5 embryonic days in age and older mimics the expression pattern of retinoic acid receptor, beta (Rarb). Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When maintained as homozygotes, this strain can lose beta-galactosidase staining capacity. The donating investigator reports that lacZ staining is lost when their mice are bred onto the C57BL/6 genetic background. This strain serves as a reporter strain, with retinoic acid signaling being indicated by beta-galactosidase activity. This mutant mouse strain may be useful in tracking retinoic acid signaling pattern. | ||
| 009606 | STOCK Tg(Six2-EGFP/cre)1Amc/J | Repository- Live |
| Hemizygous Six2-TGCtg mice are viable and fertile, harboring a BAC transgene with a Tet-off-eGFPCre under control of the Six2 promoter/enhancer regions within the BAC transgene. The Tet-off-eGFPCre contains both the tetracycline-controlled transactivator protein (tTA) as well as the tetracycline operator (tetO; also called tetracycline-responsive element [TRE] or tet-operator) upstream of an EGFPCre fusion protein. Sequence updates published after this transgene was created revealed an upstream Six2 ATG start site that is in-frame with the tTA; thus an apparent and unintended fusion was created with the tTA (80 amino acids potentially added) that renders transgene expression unaffected by tetracycline/doxycycline administration. It is not known whether the "mutant" tTA is resistant to tetracycline/doxycycline inactivation and still binds to TRE to activate EGFPCre expression or if the TRE acts as a minimal promoter adjacent to the Six2 promoter in the pr ..... For more information please see the full phenotype on the strain data sheet | ||
| 004783 | STOCK Tg(Sox2-cre)1Amc/J | Repository- Live |
| Mice hemizygous for the Sox2Cre transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse SRY-box containing gene 2 promoter. When these transgenic mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in Sox2-expressing tissues in the offspring. Specifically, Cre recombinase activity is detected in the epiblast cells at embryonic day 6.5, with little or no activity in other cells at gastrulation. Some activity is also detected in extra embryonic derivatives of the epiblast, the yolk sac mesoderm and amnion. No Cre recombinase activity is detected in primitive endoderm derived tissues, visceral endoderm. The phenotype of homozygous mice has not been characterized to date (April 2011). These Sox2Cre transgenic may be useful for generating epiblast-derived specific ..... For more information please see the full phenotype on the strain data sheet | ||
| 008208 | STOCK Tg(Stra8-cre)1Reb/J | Repository- Live |
| Homozygous Stra8-cre transgenic mice are viable and fertile, with expression of an optimized variant of Cre recombinase (iCre) directed by the 1.4 kb mouse Stra8 (stimulated by retinoic acid gene 8) genomic promoter fragment. Postnatal Cre-recombinase expression is first observed in testes at postnatal day (P)3 (early-stage spermatogonia), increases out to P7 (pre-leptotene-stage spermatocytes), and is not detected in other tissues examined (including ovaries). In addition, no cre expression is reported in male or female embryos. When Stra8-cre transgenic males are bred with female mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence, specifically during these stages of spermatogenesis. These Stra8-cre transgenic mice may be useful in generating conditional knockouts in postnatal, premeiotic, male germ cells for studying spermatogenesis. | ||
| 013752 | STOCK Tg(TCF/Lef1-HIST1H2BB/EGFP)61Hadj/J | Repository- Live |
| TCF/Lef:H2B-GFP transgenic mice express an H2B-EGFP fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Green Fluorescent Protein gene) under the control of six copies of a T cell specific transcription factor/lymphoid enhancer-binding factor 1 (TCF/Lef1) response element and a heat shock protein 1B (Hspa1b) minimal promoter. Mice homozygous for the transgene are viable, fertile, and normal in size. Wnt (wingless-related MMTV) family members are required for triggering embryonic axis formation and for proper development. Wnt signaling results in phosphorylation and nuclear localization of β-catenin, a transcriptional co-activator protein, which, together with the TCF/Lef family of transcription factors, induces the transcription of downstream genes. TCF/Lef:H2B-GFP transgenic mice contain 6 copies of nuclear localized TCF/Lef1 DNA binding sites, which provides increased sensitivity to Wnt/&be ..... For more information please see the full phenotype on the strain data sheet | ||
| 003658 | STOCK Tg(TIE2GFP)287Sato/J | Repository- Live |
| This strain expresses Green Fluorescent Protein (GFP) under the direction of the endothelial-specific receptor tyrosine kinase (Tek, formerly, Tie2) promoter. Endothelial cells expressing GFP can be visualized via fluorescent microscopy or purified by FACS. | ||
| 007788 | STOCK Tg(Thy1-EGFP)MJrs/J | Repository- Live |
| Mice harboring the Thy1-GFP transgene are viable and fertile with enhanced green fluorescent protein (EGFP) expression under the control of a modified Thy1 promoter region (containing the sequences required for neuronal expression but lacking the sequences required for expression in non-neural cells). Homozygous or hemizygous Thy1-GFPM mice (derived from founder line M) express EGFP in sparse subsets of neurons within specific populations; providing a bright, vital Golgi-like stain. Less than 10% of all motor axons, retinal ganglion cells, lumbar dorsal root ganglion, and cortex express EGFP. These Thy1-GFPM transgenic mice may be useful in neurobiological studies for fluorescent labeling of neural tissues, especially for mossy fibers in the cerebellum and intense, yet sparse, labeling of a variety of neuronal subsets.
This strain is one of many from the donating investigator with specific/differential fluorescent protein expression in neural tissues (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 012708 | STOCK Tg(Thy1-cre/ERT2,-EYFP)HGfng/PyngJ | Repository- Live |
| The "single-neuron labeling inducible Cre-mediated knockout" (SLICK) transgene is designed with two separate copies of the modified mouse Thy1 promoter region (each containing the sequences required for neuronal expression but lacking the sequences required for expression in non-neural cells) driving expression of a CreERT2 fusion protein and an enhanced yellow fluorescent protein (EYFP). Mice harboring the SLICK transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. mRNA coexpression of both CreERT2 and YFP is highly correlated. YFP expression is directed primarily to projection neurons with variations in the extent and brightness of labeling observed in different founder lines (see below). CreERT2 fusion gene activity is inducible; observed at high levels following tamoxifen administration (see below). When SLICK transgenic mice are bred with mice containing loxP-flanked seque ..... For more information please see the full phenotype on the strain data sheet | ||
| 011108 | STOCK Tg(Ttr-RFP)1Hadj/J | Repository- Live |
| Mice hemizygous for the Ttr-RFP transgene are viable and fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice exhibit reduced viability on this background. Expression of the transgene is directed to the visceral endoderm of early postimplantation embryo (E5.5), yolk sac endoderm, and other endoderm-derived organs including intestine, liver, pancreas, and stomach. In addition, expression is detected in the optic vesicle and regional areas of the brain. This mutant mouse strain may be useful for in vivo imaging, fate mapping and genetic modification of cells of the visceral endoderm. | ||
| 008199 | STOCK Tg(dlx6a-cre)1Mekk/J | Repository- Live |
| Homozygous Dlx5/6-Cre transgenic mice are viable and fertile. Expression of Cre recombinase (Cre) is directed to differentiating and migrating forebrain GABAergic neurons during embryonic development by the I56i and I56ii enhancers from the zebrafish dlx5a/dlx6a intergenic region (with the 5' promoter region of zebrafish dlx6a in place to increase the activity of the intergenic enhancers rather than direct tissue-specific expression). When Dlx5/6-Cre transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence in the offspring in the offspring. These Dlx5/6-Cre transgenic mice may be useful in generating specific deletions of floxed alleles in GABAergic forebrain neurons. | ||
| 012345 | STOCK Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo/J | Repository- Live |
| Hemizygous TRE-Bi-SG-T line 1.1 transgenic mice are viable and fertile, with no reported phenotypic abnormalities. The TRE-Bi-SG-T transgene has Myc-tagged tdTomato and full-length mouse synaptophysin/mut4EGFP fusion protein (Syp-GFP) expression under the control of the bi-directional tet-responsive promoter (tetO or TRE). When bred with another mouse expressing tetracycline-controlled transactivator protein (tTA) or reverse tetracycline-controlled transactivator protein (rtTA), tdTomato and Syp-GFP fusion protein expression can be regulated with the tetracycline analog doxycycline (dox) in the resulting double mutant offspring. In tTA(-dox) or rtTA(+dox)-expressing cells, tdTomato expression is directed to the entire cell, while GFP expression is directed to the synapse/synaptic vesicle. The donating investigator reports that direct GFP fluorescence and direct tdTomato fluorescence may be visualized in these mice when tTA is present/dox is absent. In addition, the three Myc epi ..... For more information please see the full phenotype on the strain data sheet | ||
| 013115 | B6.Cg-Rag1tm1Mom Tg(UBC-GFP)30Scha/J | Research Strain |
| Tg(UBC-GFP)30Scha generates green fluorescent protein (GFP) expression in all cells of the body with cell lineage-specific variation in expression level. Hematopoetic cells have high expression levels compared with other cells and T cells have a 2-fold higher GFP expression level than that in CD19+B220+ B cells. The Rag1tm1Mom targeted disruption, which blocks the intragenic recombination essential for T and B cell antigen receptor formation, leaves homozygous mice immunocompromised due to the inability to form functional, mature T and B cells. This strain, which combines this targeted mutation with this transgene, is a universal host that does not reject engraftment regardless of histoincompatibility and permits detection of host-derived embryos or tissues by detection of GFP, as long as the donor tissue is not also engineered to express GFP. Animals from this strain can also be used as sentinel mice in specific pathogen free environments. ..... For more information please see the full phenotype on the strain data sheet | ||
| 000516 | C57BLKS-Rpl24Bst/J | Research Strain |
| Belly spot and tail (Rpl24Bst) is a semidominant, homozygous in utero lethal mutation. Adult heterozygotes are viable and fertile although a reduced birth rate for heterozygotes has been reported. This may reflect incomplete penetrance, or, more likely, prenatal mortality. The Rpl24Bst mutation has variable expressivity and the heterozygous phenotypic traits include shortened and kinked tail, white feet and belly spot, malocclusion, smaller body size, exencephaly, abnormalities of the spine, ocular defects, and polydactyly. Polydactyly is found predominantly in the right rear paw, occasionally in the left front paw and rarely in the left rear or right front paws. Approximately 50-60% of the heterozygotes have a reduction in pupillary light reflex in one or both eyes due to an underlying optic nerve atrophy. Ontological studies showed a delay in the developmental fusion of the optic fissure, a disruption of the retinal layers by embryonic day 1 ..... For more information please see the full phenotype on the strain data sheet | ||
| 002484 | 129-Alpltm1Sor/J | Cryopreserved - Ready for recovery |
| Mice heterozygous for the Alpltm1Sor targeted mutation appear normal and viable. Homozygous mutant mice are perinatal lethals but can be rescued by pyridoxal treatment. Surviving homozygotes develop epilepsy due to reduced GABA levels in the brain. Bone formation does not appear to be grossly affected in untreated animals, but treated animals exhibit cranial dysmorphology. The targeting vector contained both a b-galalactosidase and a neomycin genes (beta-geo), both of which are under the control of the Alpl promoter and are thus expressed in a tissue specific manner. Specifically, expression occurs in developing bones and in primordial germ cells (PGC), and the beta-galactosidase thus serves as a marker for these tissues. The marker for PGC's is particularly significant because the current marker (alkaline phosphatase staining) is only useful to study early germ cell migration. | ||
| 002292 | 129-Gt(ROSA)26Sor/J | Cryopreserved - Ready for recovery |
| Mice heterozygous or homozygous for the ROSA26 retroviral insertion display no distinguishing phenotype. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult mouse. Formerly named TgR(ROSA26)26Sor. | ||
| 005483 | 129-Tg(CAG-EYFP)7AC5Nagy/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for the transgenic insert (founder line 7AC5/EYFP) are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Homozygotes fluoresce with twice the intensity of the hemizygotes. Notable exceptions to this phenotype are erythrocytes and adipocytes in which fluorescence is negligible or absent. Please note, the original publication describing the creation and phenotype of mice harboring the pCX::EFYP transgene (Hadjantonakis 2002 BMC Biotechnol 2002 2:11) describes mice from founder line "YC5/EYFP" and indicates they are available through The Jackson Laboratory as Stock No. 003772. While the 003772 strain is no longer available, the donating investigators report that this Stock No. 005483 strain (from founder line "7AC5/EYFP") was generated from embryonic stem cell clones from the same experiment using the ..... | ||
| 007664 | 129S-Efnb1tm1Sor/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites flanking exons 2 through 5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 5 deleted in the cre-expressing tissue(s). These Efnb1 conditional mutant mice may be useful in studying cellular signaling in embryonic development and adult mice; specifically receptor tyrosine kinases. For example, when crossed to a strain expressing Cre recombinase in epiblast-derived tissues (see Stock No. 003755), this mutant mouse strain may be useful in embryogenesis research. For example, when bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 007807 or For more information please see the full phenotype on the strain data sheet | ||
| 003383 | 129S-Nogtm1Amc/J | Cryopreserved - Ready for recovery |
| Homozygous mice are born but die shortly after birth, exhibiting multiple defects, including an open neural tube, skeletal abnormalities, shortened body axis, and a small vestigial tail. Analysis of early gene expression has shown that the loss of Nog expression in the floorplate, notochord, and roofplate results in a progressive failure of ventral development in the CNS and somites. Nog is also expressed in condensing cartilage in the limb and in the sclerotome of somites so its loss results in defects in cartilage patterning and skeletal morphogenesis. Heterozygous embryos show lacZ reporter expression in pattern consistent with the endogenous gene. | ||
| 008002 | 129S-Pafah1b1tm2Awb/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites flanking exons 3 through 6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Histological analysis of brains from homozygotes reveals slightly wider CA1 and CA3 regions and a split in CA2 region in the hippocampus. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3 through 6 deleted in the cre-expressing tissue(s). | ||
| 007199 | 129S-Sgpl1Gt(ROSA)78Sor/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this mutant allele have reduced size and weight gains after birth and do not survive past 8 weeks of age. Homozygotes occur at a lower than Mendelian ratio (19%) from heterozygote X heterozygote crosses. No gene product is detected in homozygous embryos aged ED9.5-12.5 or in adult gonad. Beta-galactosidase staining pattern mimics the endogenous gene expression pattern in adult intestinal epithelial cells. Homozygous embryos E11.5 to E18.5 exhibit hemorrhages and microaneurisms. Vascular defects persist into adulthood. At 6 weeks of age, mutant mice are anemic (low hemoglobin concentration, reduced red blood cell count, low hematocrit). Mutants exhibit polychromasia (abnormally high number of immature blood cells), kidney defects (blood urea nitrogen level abnormally high, kidney size smaller than wildtype, swollen blood filled glomeruli, reduced number of vascular smooth muscle cells) and abnormalities in palate bone fusion. Homozygotes are infertile. Heterozygote ..... For more information please see the full phenotype on the strain data sheet | ||
| 009085 | 129S/Sv-Rettm1Cos/J | Cryopreserved - Ready for recovery |
| The Ret- allele (also called ret-k-, ret-k minus, or c-ret-) disrupts the region of the ret proto-oncogene (Ret; also called ret-k or c-ret) locus harboring the invariant lysine codon required for Ret kinase activity. Homozygous mice die around 16-24 hours after birth, exhibiting abnormalities in kidney/urinary (renal agenesis/hypodysplasia) and peripheral nervous system development (including sympathetic, parasympathetic, and enteric ganglia), as well as abnormal enteric neural crest cell migration. Because homozygous mice lack enteric ganglia from the hindgut, these mice are also a model of Hirschsprung's Disease. | ||
| 002303 | 129S2/SvPas-Smarcad1Gt(6LSN)6028Gos/J | Cryopreserved - Ready for recovery |
| 008149 | B6(Cg)-Snord116tm1.1Uta/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this Snord116del (1-loxP or knockout) allele are viable and fertile. As the Snord116 gene cluster is imprinted and expressed only from the paternal allele, mice with paternal inheritance of the deletion lack expression of the targeted Snord116 small nucleolar RNAs (snoRNAs) gene cluster in brain tissues. Similarly, paternal transmission of the mutant allele is required to obtain the mutant phenotype in offspring. Affected heterozygotes (paternal deleted/maternal wildtype) recapitulate a subset of Prader-Willi syndrome (PWS) characteristics, including early-onset postnatal growth retardation, delayed sexual maturation, increased anxiety, motor learning deficit and hyperphagia (but not obesity). Other reported abnormalities include altered metabolic fuel usage, prolonged meal time, and increased levels of circulating ghrelin. These Snord116del mice may be useful in studying growth and feeding regulation, mechanisms of obesity, and pa ..... For more information please see the full phenotype on the strain data sheet | ||
| 008118 | B6(Cg)-Snord116tm1Uta/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this 2-loxP (floxed) allele are viable and fertile, with loxP sites flanking the Snord116 small nucleolar RNAs (snoRNAs) gene cluster. When bred to mice that express Cre recombinase, the resulting offspring will have this gene cluster deleted in the cre-expressing tissue(s). Because the Snord116 gene cluster is imprinted and only expressed from the paternal allele, breeding 2-loxP males with cre-expressing females may be required to generate deleted offspring with the knockout phenotype. The donating investigator reports that the distance between the two loxP sites (~140 kb) may reduce the recombination efficiency in somatic cells. As deletions of the Snord116 cluster are associated with Prader-Willi syndrome (PWS), mice carrying the 2-loxP (floxed) allele may be useful in generating conditional mutations for studying the role of Snord116 in growth and feeding regulation, mechanisms of obesity, and patho ..... For more information please see the full phenotype on the strain data sheet | ||
| 006203 | B6.129(FVB)-Ahrtm3.1Bra/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Hepatic protein expression of the conditional allele (before exon 2 excision) is equivalent to wildtype by Western blot analysis. This strain may be useful in generating tissue-specific mutants of the floxed allele for use in studies including teratogenesis and xenobiotic metabolism (including dioxin and PCB), Per-Arnt-Sim transcription factors, and fetal vascular development such as ductus venosus closure.
When bred to a strain expressing Cre recombinase in hepatocytes (see Stock No. 003574 for example), this mutant mouse strain may be useful in studies of toxicology. When bred to a strain expressing Cre recombinase in endothelial cells (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 006257 | B6.129-Aldh5a1tm1Kmg/J | Cryopreserved - Ready for recovery |
| Homozygous mutation of this gene results in reduced body weight, ataxia, seizures, gliosis of the hippocampus, and eventual status epilepticus. From 19-26 days of age, repetitive tonic-clonic seizures results in more than 95% mortality. Biochemical assays shows complete ablation of the endogenous enzymatic activity in the brains, livers, hearts, and kidneys of homozygous mutant mice. Homozygotes have increased levels of GHB and GABA in liver and brain tissues, as well as in urine. Phenotype can be rescued to varying degrees utilizing a number of both pharmacotherapeutic and gene therapeutic approaches. Although heterozygous mice have approximately 50% of the endogenous enzyme activity compared to wildtype mice, they are viable and fertile. Mice with this targeted mutation may be useful in studying succinate semialdehyde dehydrogenase (SSADH) deficiency and to explore the effect of GABA and GHB accumulation on central nervous system development and function. | ||
| 011036 | B6.129-Hoxa11tm1Dmwe/J | Cryopreserved - Ready for recovery |
| This strain expresses Green Fluorescent Protein from the targeted locus. Localization of Green Fluorescent Protein closely mimics the endogenous gene (mRNA) expression pattern. Fluorescence is first detected at embryonic day 9.5 in the tail tip and forelimb bud. Fluorescence is later detected in the tail, neural tube and limb buds. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are infertile. | ||
| 005697 | B6.129-Otx1tm4(cre)Asim/J | Cryopreserved - Ready for recovery |
| This strain expresses Cre recombinase from the targeted locus. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes have a perinatal lethal phenotype. Expression of the Cre recombinase gene (mRNA) under the control of the endogenous gene promoter, is detected in the lateral midbrain of embryonic day 10.5 aged embryos and in the presumptive alar-basal plate boundary of embryonic day 12.5 aged embryos, closely mimicking the endogenous gene expression pattern. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination is first detected at embryonic day 8.7. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study developmentally critical gene function in the brain. | ||
| 006879 | B6.129-Scd2tm1Myz/J | Cryopreserved - Ready for recovery |
| While heterozygous mice are viable and fertile, mice homozygous for this targeted allele die within 24 hours of birth. Brain tissues from homozygous mice show no expression from the targeted gene. Homozygotes exhibit neonatal lethality with 100% penetrance on this genetic background (less penetrant on 129SvEv genetic background) likely due to severe skin permeability barrier abnormalities. Null mice also have abnormal epidermal morphology and abnormal lipid homeostasis in the skin and liver. These mutant mice may be useful in studying monounsaturated fatty acid synthesis, lipid biosynthesis and metabolism, cholesterol homeostasis, and skin disease, as well as obesity and diabetes. | ||
| 002463 | B6.129S-Itga4tm1Hyn/J | Cryopreserved - Ready for recovery |
| Mice homozygous for the Itga4tm1Hyn targeted mutation die during embryonic development. Homozygous mutant embryos fail to fuse the allantois with the chorion during placentation. There is a defect in the epicardium and coronary vessels results in in utero cardiac hemorrhage; also known as CD49D, VLA-4. | ||
| 002274 | B6.129S-Itga5tm1Hyn/J | Cryopreserved - Ready for recovery |
| Mice homozygous for the Itga5tm1Hyn targeted mutation die during embryonic development. Homozygous mutant embryos exhibit defects in the vasculature of the yolk sac and the embyro as well as severe defects in posterior and extraembryonic mesoderm. Implantation and initiation of gastrulation and neurulation is normal. There is normal development of notochord, somite and considerable development of brain, optic and otic anlagen and branchial arches. | ||
| 009367 | B6.129S1-Bptftm1.1Cwu/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s). This mutant mouse strain may be useful in generating conditional mutations for studying chromatin remodeling during development. | ||
| 006490 | B6.129S4-Abcb7tm1Mdf/J | Cryopreserved - Ready for recovery |
| Homozygous mice are viable and fertile with no reported neurological or hematological abnormalities. These mutant mice have loxP sites flanking exons 9 and 10 of the endogenous gene. When bred to Cre recombinase expressing mice, exons 9 and 10 are deleted in the offspring dependent on the tissue specificity of the Cre recombinase expressing parent. The donating investigator reports that the null allele is not transmissible due to an effect on the extraembryonic tissues. This mutant may be useful in studying cytosolic Fe-S cluster assembly and metabolism, Friedreich ataxia, anemia, and hematopoiesis.
When bred to a strain expressing Cre recombinase in liver (see Stock No. 003574 for example), this mutant mouse strain may be useful in studies of hepatocyte iron metabolism.
When bred to a strain expressing Cre recombinase in epiblast derived cells (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 005897 | B6.129S4-Ppardtm1Rev/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 4 of the targeted gene is deleted in the tissue of interest, leading to premature termination of the translation product upstream of the DNA binding domain. This strain may be useful in generating tissue-specific mutants of the floxed allele for use in studies including embryo development, adipocyte physiology, fat metabolism and storage, inflammation, and cancer. | ||
| 006142 | B6.129S4-Ppargtm1Rev/J | Cryopreserved - Ready for recovery |
| All mice homozygous for this targeted mutation die after gestational day 9.5 from severe placental defects and myocardial thinning. Heterozygotes are viable and fertile. White adipose tissue from heterozygous mice display approximately half the mRNA expression compared to wildtype. Tracer-determined glucose disposal rates and hepatic glucose production show that peripheral tissues and livers from heterozygotes are more sensitive to the effects of insulin than wildtype. This mutation eliminates both DNA-binding and ligand-binding functions of the endogenous gene, concomitantly generating a lacZ reporter that faithfully recapitulates the endogenous expression pattern. Heterozygous mice or homozygous embryo-derived cells may be useful in studies of embryo and placental development, diabetes, atherosclerosis, inflammation, and for beta-galactosidase reporter function of the endogenous gene. | ||
| 002741 | B6.129S7-Alpltm1Sor/J | Cryopreserved - Ready for recovery |
| Mice heterozygous for the Alpltm1Sor targeted mutation appear normal and viable. Homozygous mutant mice are perinatal lethals but can be rescued by pyridoxal treatment. Surviving homozygotes develop epilepsy due to reduced GABA levels in the brain. Bone formation does not appear to be grossly affected in untreated animals, but treated animals exhibit cranial dysmorphology. The targeting vector contained both a b-galalactosidase and a neomycin genes (beta-geo), both of which are under the control of the Alpl promoter and are thus expressed in a tissue specific manner. Specifically, expression occurs in developing bones and in primordial germ cells (PGC), and the beta-galactosidase thus serves as a marker for these tissues. The marker for PGC's is particularly significant because the current marker (alkaline phosphatase staining) is only useful to study early germ cell migration. | ||
| 006039 | B6.129S7-Efnb2tm1And/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this targeted mutation show growth retardation and enlargement of the heart at embryonic day 10 (E10), with 100% lethality occurring around E11. Reporter protein expression patterns are consistent with arterial, but not venous, expression of the endogenous gene; prominent lacZ signal is observed in hindbrain and somites, with lower levels in aorta and heart as early as E8.25. Expression in the yolk sac was first detected at E8.5, and is also observed in nephrogenic mesoderm and branchial arches. Homozygous embryos show defective angiogenic remodeling at the capillary plexus stage in both yolk sac and head. Endothelial vessel support cell differentiation of the yolk sac is also defective. Homozygotes lack myocardial trabecular extensions, and capillary ingrowth into the neural tube does not occur. Heterozygous mice are viable, fertile, exhibit no behavioral defects, and have identical lacZ expression patterns. These mutant mice may be useful in studying ..... For more information please see the full phenotype on the strain data sheet | ||
| 006042 | B6.129S7-Efnb2tm2And/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 1 of the targeted gene is deleted in the tissue of interest. These mutant mice may be useful in studying the cellular and molecular mechanisms underlying vasculogenesis and angiogenesis, the topography of neovascularization, and adult neovascularization, including tumor angiogenesis. To test the effectiveness of this model, these mutant mice were bred to an endothelial-specific Cre-expressing transgenic mice, Tg(Tek-cre)12Flv (Stock No. 004128) . Offspring homozygous for the Cre-mediated exon 1 deletion show angiogenic remodeling defects and embryonic death identical to homozygous Efnb2tm1And mice (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 009081 | B6.129X1-Id1tm1Xhsu/J | Cryopreserved - Ready for recovery |
| Homozygous Id1/GFP mice (Id1GFP/GFP) are viable and fertile; harboring an enhanced green fluorescent protein (EGFP) "knock-in" allele that both abolishes endogenous Id1 gene function and expresses EGFP from the Id1 promoter/enhancer elements. As such, EGFP fluorescence is directed to Mac1+/Ly6G+ myeloid lineage bone marrow cells (although rare fluorescence is reported in B220+ and/or CD19+ bone marrow cells). Homozygotes exhibit decreased long-term repopulating of hematopoietic stem cell (HSC) populations and a ~40% reduction in SLAM positive HSC. These Id1/GFP mutant mice allow fluorescent monitoring of Id1 expression in the bone marrow (granulocyte and macrophage progenitors as well as downstream myeloid lineage cells) and may be useful for studying HSC maintenance and myeloid-versus-lymphoid lineage decisions. NOTE:: Because the Id1GFP mutation originated in 129X1-derived ES cells that harbor the ..... | ||
| 006072 | B6.129X1-Mcl1tm2Sjk/J | Cryopreserved - Ready for recovery |
| Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice are embryonic lethal. These mutant mice may be useful in studies of immune function, including apoptosis, B and T cell development, and bone marrow cell differentiation. When bred to a strain with loxP sites inserted into the same targeted allele (Stock No. 006088) and a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 004126), this mutant mouse strain may be useful in studies of lymphocyte development. When bred to a strain with loxP sites inserted into the same targeted allele (Stock No. 006088) and a strain expressing interferon inducible Cre recombinase in t ..... | ||
| 009642 | B6.Cg(129)-Tg(Gh1-cre)1Sac/J | Cryopreserved - Ready for recovery |
| These transgenic mice express Cre recombinase under the control of the human Growth Hormone (GH1) Locus Control Region and the rat growth hormone 1, (Gh1) promoter. When crossed with a reporter strain, Cre recombinase expression is seen during development and in the adult in pituitary somatotropes. Weaker expression is detected in lactotropes. Cre expression is detected in the forelimb skin of embryos aged embryonic day 16.5-17.5, and weaker expression in adult kidney, ovary, and testis. Southern blot analysis reveals approximately 50 copies or the transgene segregated to one locus. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study developmentally critical gene function in the anterior pi ..... For more information please see the full phenotype on the strain data sheet | ||
| 012360 | B6.Cg-Erbb4tm1.1(cre/ERT2)Aibs/J | Cryopreserved - Ready for recovery |
| Mice heterozygous for the Erbb4-2A-CreERT2 allele are viable and fertile, with a viral 2A oligopeptide that mediates ribosomal skipping and a CreERT2 fusion protein (CreERT2 fusion protein) inserted immediately downstream of the translational STOP codon of the Erbb4 locus (v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)). As such, Erbb4-2A-CreERT2 mice have both endogenous gene and CreERT2 fusion protein expression directed to Erbb4-expressing cells. Cre-ERT2 fusion gene activity is inducible; observed following tamoxifen administration. When Erbb4-2A-CreERT2 mice are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in the Erbb4-expressing cells of the double mutant offspring. Specifically, the donating investigator reports that following tamoxifen induction, Erbb4-2A-CreERT2 directs reporter gene expression in scattered interneuron s ..... For more information please see the full phenotype on the strain data sheet | ||
| 007920 | B6.Cg-Gt(ROSA)26Sortm2(CAG-EYFP)Hze/J | Cryopreserved - Ready for recovery |
| Ai2 mice hemizygous for this Rosa-CAG-LSL-EYFP conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream enhanced yellow fluorescent protein (EYFP). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of EYFP. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, EYFP expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai2 mice do not express EYFP prior to introduction of Cre recombinase and EYFP fluorescence following exposure to cre is weak but easily detected by mRNA (in situ hybridization) and antibody staining (immunohistochemistry). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-mediated ..... For more information please see the full phenotype on the strain data sheet | ||
| 007942 | B6.Cg-Isl2tm1Arbr/J | Cryopreserved - Ready for recovery |
| Mice homozygous for the Isl2DTA targeted mutation are viable and fertile. These mice have the diphtheria toxin (DTA) gene inserted into the Isl2 (insulin related protein 2 (islet 2)) locus. Expression of DTA in Isl2-expressing cells is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTA expression and subsequent cell ablation.
When bred to a strain expressing Cre recombinase in motor neurons (see Stock No. 006600 for example), this mutant mouse strain may be useful in neurodevelopmental studies. These Isl2DTA mutant mice are available on a STOCK genetic background (Stock No. 007603), as well as a C57BL/6J-backcrossed background (Stock No. 007942 ..... | ||
| 006200 | B6.Cg-Tnks2tm1.1Yjc/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for the targeted mutation are viable and fertile. No gene product (mRNA) is detected by RT-PCR analysis of activated spleen lymphocytes. While neither telomere shortening nor chromosomal abnormalities (even across multiple generations) are observed, homozygous mice have significantly decreased body weight. These mutant mice may be useful in studies of both telomerase function and telomerase-independent pathways which affect development and metabolism. | ||
| 009352 | B6.Cg-Tg(CDX2-cre*)189Erf/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for the CDX2P9.5-G22Cre transgene are viable and fertile. The 9.5 kb human caudal type homeo box 2 (CDX2) promoter/enhancer sequence is prevented from driving nuclear-localized Cre recombinase expression as a 22 guanine nucleotide repeat tract between initiating methionine (ATG) codon and the remainder of the coding region alters the cre reading frame. Presumably, following a somatic mutation that results in some frameshift mutation(s) in the guanine nucleotide tract in a subset of somatic cells, resultant expression of a functional Cre recombinase is observed predominantly in colonic epithelium during late gestation and in adult tissues. Specifically, mosaic Cre recombinase expression would be observed in epithelium from the distal ileum and cecum, and mainly the proximal and distal colon from the crypt base to the luminal surface. Cre recombinase expression may also be observed in a few caudal-derived cells during early development. When these transgenic ..... For more information please see the full phenotype on the strain data sheet | ||
| 008221 | B6.Cg-Tg(IGFBP1)2Miel/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for the hIGFBP-1 transgene are viable and fertile with no reported gross morphological or developmental changes. The hIGFBP-1 transgene encompasses the entire human IGFBP-1 structural gene and its regulatory sequences, allowing transgene expression of IGFBP-1 to remain responsive to normal hormonal regulation. Transgenic mice overexpress hIGFBP-1, with hIGFBP-1 mRNA expression in a tissue-specific fashion more similar to the human pattern than the murine pattern. Fasting transgenic mice have elevated total serum IGFBP-1 levels that fluctuate according to nutritional status (as they do in humans), and exhibit postprandial hyperinsulinemia with preservation of normal glucocompetence and insulin sensitivity. Transgenic mice also have significantly greater hyperinsulinemic response to glucose challenge and cardiovascular abnormalities in response to carbohydrate load and vasoconstrictors. Transgenic mice exhibit fasting hyperglycemia and hyperinsulinemia and glucose intoler ..... For more information please see the full phenotype on the strain data sheet | ||
| 009643 | B6.Cg-Tg(Lhb-cre)1Sac/J | Cryopreserved - Ready for recovery |
| These transgenic mice express Cre recombinase under the control of the bovine LHB, luteinizing hormone beta polypeptide promoter. Although the transgenic construct contains sequence encoding a fusion gene of EGFP and Cre recombinase, no EGFP fluorescence is detected. When crossed with a reporter strain, Cre recombinase expression is seen in mature pituitary gonadotropes, specifically in cells that produce luteinizing hormone beta. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study developmentally critical gene function in the pituitary. | ||
| 007894 | B6.Cg-Tg(Rgs4-EGFP)4Lvt/J | Cryopreserved - Ready for recovery |
| Hemizygous RGS4 BAC transgenic mice are viable and fertile. As the RGS4 BAC transgene has an IRES2-eGFP construct inserted into the 3' UTR of the regulator of G-protein signaling 4 (Rgs4) locus, transgenic RGS4 transcripts and EGFP protein expression is observed in a pattern consistent with endogenous Rgs4. While the transgene is designed to co-express EGFP and RGS4, over-expression of RGS4 is not reported to result in unfaithful reporting of endogenous RGS4 expression. Under the control of the RGS4 promoter/enhancer elements, transgene expression reports dynamic developmental, regional, and cellular specific expression in developing and mature cerebral cortex neurons across all cortical domains, as well as developing and mature subcortical regions (telencephalon, diencephalon, and brainstem). While immunostaining against the transgenic product ("RGS4-GFP") allows detailed cellular resolution of neuronal cell bodies and processes, the subcellular localization of EGFP cann ..... For more information please see the full phenotype on the strain data sheet | ||
| 006232 | B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for the CCSP-rtTA transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat Scgb1a1, secretoglobin, family 1A, member 1 (uteroglobin), gene promoter. rtTA activity detected in bronchial and type II epithelial cells of lung tissue from adult transgenic mice and in embryos from pregnant females treated with the tetracycline analog doxycycline (dox). In the latter, rtTA-induced expression of a luciferase reporter under the regulation of a tetracycline-responsive promoter (TRE; tetO) has been detected as early as embryonic day 12.5. When hemizygotes are mated to a second transgenic strain carrying a gene of interest under the regulatory control of a TRE, expression of the target gene in the bitransgenic offspring can be regulated by dox; in the presence of dox, transcription of the target ..... For more information please see the full phenotype on the strain data sheet | ||
| 004604 | B6;129-Ctnna1tm1Efu/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
When bred to a strain expressing Cre recombinase in the female germline (see Stock No. 003551 for example), this mutant mouse strain may be useful in studies of mammary epithelium. When bred to a strain expressing Cre recombinase in the mammary gland (see Stock No. 003552 for example), this mutant mouse strain may be useful in studies of mammary epithelium. When bred to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771 for example), this mutant mouse strain may be useful in studies of the cerebral cortex and the hedgehog signalling pathway. | ||
| 006904 | B6;129-Msctm1Eno/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this MyoR mutant allele are viable and fertile with no obvious abnormalities. These mice may be useful in studying muscle development, specifically craniofacial muscles. For example, when these mice are bred with capsulin-mutant mice, the resulting double mutant offspring have significant abnormalities in craniofacial (and other) muscle development and MyoD-family transcription factor gene expression. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available. | ||
| 004693 | B6;129-Wnt7btm1Parr/J | Cryopreserved - Ready for recovery |
| Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice have an embryonic lethal phenotype, failing to develop past 11.5 days post coitum (d.p.c.). Placental formation is defective due to the failure of the chorion and the allantois to fuse. Morphological disorganization of the chorion is observed by 8.0-8.25 d.p.c. Integrin alpha 4 protein is not detectable in homozygous mutant chorionic cells by immunohistochemical analysis. This mutant mouse strain may be useful in studies related to the failure of chorion and allantois fusion during placental development. | ||
| 010988 | B6;129P-Cyp11a1tm1(GFP/cre)Pzg/J | Cryopreserved - Ready for recovery |
| A GFP-Cre cassette (GC) was inserted into the Cyp11a1, cytochrome P450, family 11, subfamily a, polypeptide 1, locus. Cre activity is detected in male testes, male and female adrenal glands and some kidneys from heterozygous E15.5 embryos. No green fluorescence was detected by direct fluorescence microscopy, however, immunohistochemical analysis revealed GFP expression. Transgene expression mimics the endogenous expression pattern. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous. This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 010985 | B6;129P-Klf3tm1(cre/ERT2)Pzg/J | Cryopreserved - Ready for recovery |
| These Klf3-GCE mutant mice have a tamoxifen inducible Cre-mediated recombination system. A GFP-Cre/ERT2 cassette (GCE) was inserted into the locus. Tamoxifen administration induces Cre recombination and Green Fluorescent Protein is expressed in the Klf3 domain. Weak green fluorescence is detected in the back, limbs and kidney of E15.5 heterozygous embryos. Upon induction, Cre recombinase activity is detected in kidney from E15.5 heterozygous embryos. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous.
This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 010984 | B6;129P-Upk1btm1Pzg/J | Cryopreserved - Ready for recovery |
| These mutant mice express Red Fluorescent Protein from the endogenous Upk1b (uroplakin 1B) locus. Red fluorescence is detected in the bladder and gonads of the urogenital system from E15.5 heterozygous embryos. Fluorescence mimics the endogenous expression pattern. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous.
This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 008333 | B6;129P2-Dldtm1Ptl/J | Cryopreserved - Ready for recovery |
| Mice heterozygous for the Dld (dihydrolipoamide dehydrogenase or E3 component) targeted mutation are viable and fertile. Heterozygous mice exhibit approximately half of wild-type enzymatic activity levels for E3 and all affected mitochondrial multienzyme complexes. Heterozygotes (on a C57BL/6;129P2 genetic background) exhibit increased vulnerability to treatments with MPTP (dopaminergic neurotoxin used to induce Parkinson's disease-like lesions), malonate (inhibitor of cellular respiration used to mimic Huntington's disease features), and 3-NP (mitochondrial toxin used to mimic Huntington's disease features). Homozygous mice exhibit normal development and metabolism during the preimplantation period, explained by the persistence of E3 enzyme from the oocyte. Homozygotes exhibit developmental delays shortly after implantation (7.5 to 8.5 days postcoitum (dpc)) and cease development within the subsequent two days. These Dld mutant mice may be useful to study early murine em ..... For more information please see the full phenotype on the strain data sheet | ||
| 007226 | B6;129P2-Has2tm1Jam/J | Cryopreserved - Ready for recovery |
| While heterozygous mice are viable and fertile, mice homozygous for this targeted allele die between embryonic day (E)9.5 and E10.5. Embryonic mRNA expression from the targeted gene shows only truncated transcripts of the expected length, with no full-length mRNA expression. Homozygous embryos exhibit severe cardiac and vascular abnormalities, lack hyaluronan (HA), and have yolk sac and somite deformities. Heart deformities can be rescued in explants from homozygous mice following exogenous HA or activated H-Ras treatment. These Has2 mutant mice may be useful in studying embryogenesis and development, specifically cardiac and vascular morphogenesis, as well as cell transformation. | ||
| 013139 | B6;129P2-Ifitm3tm1(RFP)Pzg/J | Cryopreserved - Ready for recovery |
| Red fluorescence is detected in ovaries from heterozygous female embryos (E15.5) and testis tissue of male heterozygous embryos. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous.
This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 006712 | B6;129P2-Olfr545tm1Mom/MomJ | Cryopreserved - Ready for recovery |
| The Olfr545tm1Mom allele has a fully intact coding sequence with a tauGFP tag permitting bicistronic expression of the endogenous gene and the green fluorescent protein tag. The olfactory sensory neurons that express this tagged allele typically project to a single dorsal-medial and a single anterior-lateral glomerulus per olfactory bulb, and, when co-expressed with Tg(P-taulacZ)8Mom expression is found not to overlap. | ||
| 006713 | B6;129P2-Olfr545tm2Mom/MomJ | Cryopreserved - Ready for recovery |
| Olfactory sensory neurons that select to express Olfr545 are labeled with tauRFP | ||
| 006716 | B6;129P2-Olfr545tm4Mom/MomJ | Cryopreserved - Ready for recovery |
| With the coding sequence of class I olfactory receptor Olfr545 excised and replaced with venusYFP, this strain allows assessment of the role of a specific olfactory receptor in olfactory sensory neuron development. Mice carrying this allele display a normal pattern of labeled axons projecting diffusely to a large subset of glomeruli in the dorsal-medial and anterior-medial olfactory bulb, but also an abnormal finding of labeled axons innervating glomeruli at the caudal margins of the olfactory bulb. Co-staining for five dorsal class I olfactory receptors shows 3.8% co-expressing class I olfactory receptor genes and the disrupted Olfr545tm4Mom allele, while co-staining for five dorsal class II olfactory receptors shows no co-expression with the disrupted Olfr545tm4Mom allele. | ||
| 006728 | B6;129P2-Vmn2r26tm2Mom/MomJ | Cryopreserved - Ready for recovery |
| Although the Vmn2r26tm2Mom allele does not express VMN2R26 protein, the vomeronasal sensory neurons actively transcribing this locus express green fluorescent protein. These neurons fail to respond, even at high concentrations, to MHC peptides or urine that stimulate VMN2R26-expressing vomeronasal sensory neurons. However, these non-responsive neurons will flux calcium normally in response to high potassium or a diacylglycerol analog, indicating normal signaling capacity. | ||
| 010539 | B6;129S-Fgf17tm1Dor/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical abnormalities. No gene product (mRNA) is detected by in situ hybridization analysis of midbrain-hindbrain from homozygous embryos. Significant portions of the inferior colliculus and the vermis cerebellum are absent in homozygotes (2 days of age and adult). The remaining midbrain and cerebellum have normal morphology although the size of the vermis cerebellum is approximately 82% of the wildtype control. The size of the rostal-most lobe of the vermis is 1/3 of the wildtype control. The fissure separating lobe III (most rostal lobe) and lobe IV does not form completely, resulting in partially fused lobes. The dorsal frontal cortex is reduced in size with a rostral shift of sensory cortical areas. Homozygotes exhibit impaired social interaction behavior. Homozygous pups vocalize less than wildtype controls when separated from mothers. Homozygous adult male ..... For more information please see the full phenotype on the strain data sheet | ||
| 006470 | B6;129S-Hopxtm1Eno/J | Cryopreserved - Ready for recovery |
| Homozygous mice are viable and fertile on this mixed genetic background. Absence of the targeted protein is confirmed in heart and brain tissues from homozygotes. The lacZ expression pattern is similar to that of the endogenous gene. Homozygous heart tissues show altered serum response factor (SRF)-associated gene expression. Mice homozygous for this null allele segregate into two phenotypic classes characterized by an excess or deficiency of cardiac myocytes. These mutant mice may be useful in studying cardiac growth and development. | ||
| 012373 | B6;129S-Hoxb1tm1(cre)Og/J | Cryopreserved - Ready for recovery |
| Mice that are heterozygous for the targeted mutation are viable, fertile, and normal in size.
The Cre recombinase expression pattern matches the endogenous gene expression pattern in heterozygous animals. Cre mediated recombination is detected in almost all tissues caudal to the hindbrain at age embryonic day 10.5 (spinal cord, peripheral nervous system, axial and appendicular musculature, and mesenchymal tissues). Mice that are homozygous for this targeted mutation allele are deficient (null) for HOXB1 and exhibit a phenotype similar to other null mutants (craniofacial defects, abnormal facial neurogenesis, facial muscle degeneration).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify ..... | ||
| 006958 | B6;129S-Nkd1tm1Kwha/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this nkd1lacZ mutant allele are viable and fertile with slightly reduced mean litter sizes. Northern blot of lung tissue, as well as western blot of embryonic tissue, confirms that the deleted exons (encoding the Dvl-binding domain) are not expressed in homozygous mutants. However, the endogenous promoter directs expression of the internal ribosome entry site-β-galactosidase (IRES-lacZ) fusion gene in a manner that closely mimics the patterns of the endogenous gene mRNAs. Because the naked cuticle (NKD) genes are considered targets for Wnt/β-catenin signaling, these nkd1lacZ mutant mice (as well as the similar nkd2lacZ mutant strain; Stock No. 006960) may be useful as a reporter for Wnt/β-catenin-dependent transcriptional activity during embryonic development or in adult mice. | ||
| 006960 | B6;129S-Nkd2tm1Kwha/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this nkd2lacZ mutant allele are viable and fertile with slightly reduced mean litter sizes. RT-PCR analysis of homozygous brain tissue confirms that the deleted exons (encoding the Dvl-binding domain) are not expressed in homozygous mutants. However, the endogenous promoter directs expression of the internal ribosome entry site-β-galactosidase (IRES-lacZ) fusion gene in a manner that closely mimics the patterns of the endogenous gene mRNAs. Because the naked cuticle (NKD) genes are considered targets for Wnt/β-catenin signaling, these nkd2lacZ mutant mice (as well as the similar nkd1lacZ mutant strain; Stock No. 006958) may be useful as a reporter for Wnt/β-catenin-dependent transcriptional activity during embryonic development or in adult mice. | ||
| 010986 | B6;129S-Osr2tm1Pzg/J | Cryopreserved - Ready for recovery |
| These Osr2-RFP mutant mice express Red Fluorescent Protein from the endogenous Osr2, odd-skipped related 2 (Drosophila), locus. Strong red fluorescence is detected the head, testis and male and female genital ducts and kidneys from heterozygous E15.5 embryos. Fluorescence mimics the endogenous expression pattern. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are not viable. The Donating Investigator reports that homozygotes die just after birth, most likely due to a cleft palate. This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 007032 | B6;129S-Wnt4tm1.1Bhr/BhrEiJ | Cryopreserved - Ready for recovery |
| This strain contains loxP sites flanking exon 2 of Wnt4 resulting in a Cre-dependent conditional null allele. Homozygotes are normal. Studies by Kobayashi et al., determined that when this conditional allele is exposed to Cre expression by Amhr2tm3(cre)Bhr Mullerian duct regression proceeds normally. | ||
| 007204 | B6;129S4-2610005L07RikGt(ROSA)73Sor/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this mutant allele (called BC058969 in the primary publication) are viable and fertile, with greater than 50% embryonic lethality observed in homozygous embryos. Homozygotes occur at a lower than Mendelian ratio (9%) from heterozygote x heterozygote crosses. No gene product is detected in homozygous embryos aged ED9.5-12.5 or in adult gonad. Homozygotes exhibit defects that affect the same cell types and processes as those controlled by the platelet-derived growth factor (PDGF) pathway, including vasculature, kidney, and skeletal defects (sternum and calvarial bones). Notably, 100% incidence of calvarial bones defects is reported. Additionally, homozygotes are reported to have low β-galactosidase activity; in situ hybridization or other sensitive methods may be necessary to detect expression of the lacZ-neo reporter fusion gene. These BC058969-mutant (2610005L07Rik-mutant) mice may be useful in studying cellular signal ..... For more information please see the full phenotype on the strain data sheet | ||
| 007208 | B6;129S4-Csrnp1Gt(ROSA)80Sor/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this mutant allele are viable and fertile, with some incidence of perinatal lethality before two weeks of age (the Donating Investigator reports 18% of homozygotes die by two weeks of age). Homozygotes have abnormalities in palate bone fusion. Homozygotes exhibit defects that affect the same cell types and processes as those controlled by the platelet-derived growth factor (PDGF) pathway, including vasculature, kidney, and skeletal defects. These mutant mice may be useful in studying cellular signaling in development and adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family) and immediate early genes (IEG) induced shortly after RTK activation. | ||
| 009046 | B6;129S4-Hras1tm1Tyj/J | Cryopreserved - Ready for recovery |
| A G12V mutant form of protein is conditionally expressed from this gene when a floxed stop segment is excised by Cre. This oncogene is involved with the development of Costello syndrome, a neuro-cardio-facio-cutaneous developmental syndrome. | ||
| 012356 | B6;129S4-Pbx3tm1Og/J | Cryopreserved - Ready for recovery |
| These Pbx3C mutant mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s). The donating investigator reports that the neo cassette is still present downstream of the floxed exon and it is unknown whether the presence of neo conveys any abnormalities. | ||
| 008204 | B6;129S4-Pou5f1tm1Jae/J | Cryopreserved - Ready for recovery |
| Mice heterozygous for this Oct4-neo mutation are viable and fertile, harboring an IRES-GFPneo fusion cassette downstream of exon 5 of the Oct4 (Pou5f1) gene. The donating investigator reports (and attempts at The Jackson Laboratory confirm) no living homozygous animals are born. While sufficient neomycin-resistance activity is observed in embryonic stem cells, no visible GFP fluorescence is reported. When treated with specific transcription factors (Oct4, Sox2, c-Myc and Klf4), some Oct4-neo murine embryonic fibroblasts (MEFs) have the properties of induced pluripotent stem (iPS) cells. Such iPS cells have the DNA methylation, gene expression and chromatin state of embryonic stem cells and can form viable chimeras, contribute to the germ line, and generate live late-term embryos when injected into tetraploid blastocysts. These Oct4-neo mutant mice may be useful for the selection of iPS cells (i.e. epigenetic reprogramming of somatic cells into pluripotent embryonic stem cells). ..... For more information please see the full phenotype on the strain data sheet | ||
| 008361 | B6;129S4-Trp53tm5Tyj/J | Cryopreserved - Ready for recovery |
| These targeted mutant mice carry a loxP-flanked STOP cassette in intron 1 that blocks expression of the gene. Both heterozygotes and homozygotes are prone to the development of tumors (primarily spontaneous thymic lymphomas and sarcomas). The latency is significantly shorter in homozygotes than in wildtype mice. Homozygotes have reduced fertility. Upon Cre-mediated recombination, the STOP cassette can be excised, leading to restoration of gene expression. This strain permits temporal control of this tumor suppressor gene, and enables studies of tumorigenesis. | ||
| 007207 | B6;129S4-Zfp640Gt(ROSA)81Sor/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this Zfp640-mutant allele are viable and fertile, with abnormalities in palate bone fusion and increased weight gain observed only in males after adolescence. Homozygotes exhibit defects that affect the same cell types and processes as those controlled by the platelet-derived growth factor (PDGF) pathway, including vasculature, kidney, and skeletal defects. Additionally, homozygotes are reported to have low β-galactosidase activity; in situ hybridization or other sensitive methods may be necessary to detect expression of the lacZ-neo reporter fusion gene. These Zfp640-mutant mice may be useful in studying cellular signaling in development and adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family) and immediate early genes (IEG) induced shortly after RTK activation. | ||
| 009326 | B6;129S6-Ext1tm1Vcs/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissues. Spurs of extra bone develop in progeny derived from a cross with an inducible Col2a1 collagen promoter Cre strain. | ||
| 002317 | B6;129S7-Alpltm1Sor/J | Cryopreserved - Ready for recovery |
| Mice heterozygous for the Alpltm1Sor targeted mutation appear normal and viable. Homozygous mutant mice are perinatal lethals but can be rescued by pyridoxal treatment. Surviving homozygotes develop epilepsy due to reduced GABA levels in the brain. Bone formation does not appear to be grossly affected in untreated animals, but treated animals exhibit cranial dysmorphology. The targeting vector contained both a b-galalactosidase and a neomycin genes (beta-geo), both of which are under the control of the Alpl promoter and are thus expressed in a tissue specific manner. Specifically, expression occurs in developing bones and in primordial germ cells (PGC), and the beta-galactosidase thus serves as a marker for these tissues. The marker for PGC's is particularly significant because the current marker (alkaline phosphatase staining) is only useful to study early germ cell migration. | ||
| 003266 | B6;129S7-Epas1tm1Rus/J | Cryopreserved - Ready for recovery |
| Mice homozygous for the Epas1tm1Rus targeted mutation develop normally until embryonic day 11.5. Beginning at embryonic day 12.5 the ratio of homozygous embryos begins to decline and by day 16.5 there are no viable mutant embryos. Overall morphological development, including the circulatory system, is normal. Of particular interest however, is a pronounced bradycardia in homozygous mutant mice. Catecholamine levels in homozygous mutant mice are also significantly lower than wildtype controls. Administration of the catecholamine precursor DOPS to pregnant females rescues approximately 40% of mutant embryos. Embryos that survive to birth appear runted, fail to nurse, and die within 24 hours of birth. These results suggest a pivotal role of EPAS1 in catecholamine homeostasis. Heterozygous mice from this strain contain a modified lacZ gene in the targeting construct. This characteristic makes this strain useful as a marker for endothelial cells. | ||
| 006044 | B6;129S7-Ephb4tm1And/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this targeted mutation show growth retardation and lack of blood flow by embryonic day 9.5-10 (E9.5-10), with lethality occurring around this time. Expression of lacZ occurs exclusively in embryonic vascular endothelial cells and is preferentially expressed on veins. Homozygous embryos show defective angiogenic remodeling at the capillary plexus stage in both yolk sac and head. Arrested cardiac morphogenesis and defective myocardial trabecular extensions are also observed. Heterozygous mice are viable, fertile, exhibit no behavioral defects, and have identical lacZ expression patterns. These mutant mice may be useful in studying the cellular and molecular mechanisms underlying vasculogenesis and angiogenesis, the topography of neovascularization, and adult neovascularization, including tumor angiogenesis. The developmental abnormalities in homozygous mice closely resemble those observed for mutant mice bearing a lacZ-expressing null mutation ..... | ||
| 009363 | B6;129X1-Cdc25atm1Hpw/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exons 1-3 in the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissues. (widespread deletion of expression is embryonic lethal). This strain may be useful in studies of development, cell cycles, and cancer in adult mice. | ||
| 009616 | B6;C3-Tg(A930038C07Rik-cre)4Aibs/J | Cryopreserved - Ready for recovery |
| Hemizygous A930038C07Rik-Tg4-Cre mice are viable and fertile, with Cre recombinase expression directed to to cortex, striatum, and cerebellum by the epidermacan (A930038C07Rik) promoter/enhancer regions within the BAC transgene. When A930038C07Rik-Tg4-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the epidermacan-expressing cells of the double mutant offspring. The donating investigators may not have assessed expression in tissues other than brain. The phenotype of homozygous mice has not yet been characterized (July 2009).
For characterization information, see images at the Allen Institute for Brain Science website (A930038C07Rik-Tg4-Cre images). | ||
| 008844 | B6;C3-Tg(Ctgf-cre)2Aibs/J | Cryopreserved - Ready for recovery |
| Hemizygous Ctgf-Tg2-Cre mice are viable and fertile, with cre expression directed to cortex and hippocampus by the Ctgf promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Ctgf-Tg2-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex and hippocampus).
For characterization information, see images at the Allen Institute for Brain Science website (Ctgf-Tg2-Cre images). | ||
| 008839 | B6;C3-Tg(Cyp39a1-cre)1Aibs/J | Cryopreserved - Ready for recovery |
| Hemizygous Cyp39a1-Tg1-Cre mice are viable and fertile, with cre expression directed to cortex, hippocampus, striatum, olfactory bulb and cerebellum by the Cyp39a1 promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Cyp39a1-Tg1-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex, hippocampus, striatum, olfactory bulb and cerebellum).
For characterization information, see images at the Allen Institute for Brain Science website (Cyp39a1-Tg1-Cre images). | ||
| 009117 | B6;C3-Tg(Cyp39a1-cre)7Aibs/J | Cryopreserved - Ready for recovery |
| Hemizygous Cyp39a1-Tg7-Cre mice are viable and fertile, with cre expression directed to cortex, hippocampus and cerebellum by the Cyp39a1 promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Cyp39a1-Tg7-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex, hippocampus and cerebellum).
For characterization information, see images at the Allen Institute for Brain Science website (Cyp39a1-Tg7-Cre images). | ||
| 008848 | B6;C3-Tg(Mybpc1-cre)2Aibs/J | Cryopreserved - Ready for recovery |
| Hemizygous 8030451F13Rik-Tg2-Cre mice (also called Mybpc1-Cre mice) are viable and fertile, with cre expression directed to scattered populations within the cortex and hippocampus by the Mybpc1 (8030451F13Rik) promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These 8030451F13Rik-Tg2-Cre (Mybpc1-Cre) mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in the cortex of the brain and hippocampus.
For characterization information, see images at the Allen Institute for Brain Science website (Mybpc1-Cre images). | ||
| 009111 | B6;C3-Tg(Scnn1a-cre)1Aibs/J | Cryopreserved - Ready for recovery |
| Hemizygous Scnn1a-Tg1-Cre mice are viable and fertile, with cre expression directed to cortex, striatum, hippocampus and cerebellum by the Scnn1a promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Scnn1a-Tg1-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex, striatum, hippocampus and cerebellum).
For characterization information, see images at the Allen Institute for Brain Science website (Scnn1a-Tg1-Cre images). | ||
| 009112 | B6;C3-Tg(Scnn1a-cre)2Aibs/J | Cryopreserved - Ready for recovery |
| Hemizygous Scnn1a-Tg2-Cre mice are viable and fertile, with cre expression directed to cortex, thalamus, midbrain and cerebellum by the Scnn1a promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Scnn1a-Tg2-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex, thalamus, midbrain and cerebellum).
For characterization information, see images at the Allen Institute for Brain Science website (Scnn1a-Tg2-Cre images). | ||
| 007975 | B6;CBA-Tg(OR8A1-taulacZ)1Mom/MomJ | Cryopreserved - Ready for recovery |
| This transgene encodes tau-linked-LacZ under the control of a minimal promoter comprised of 154 base pairs upstream of the human OR8a1 transcription start site and 146 base pairs of the 5' nontranslated exon 1. It does not drive expression of any olfactory receptor sequence. It is useful in mapping promoter elements important for human olfactory receptor expression. Histology shows mosaic expression of LacZ in the main olfactory epithelium. | ||
| 007972 | B6;CBA-Tg(Olfr151-taulacZ)4Mom/MomJ | Cryopreserved - Ready for recovery |
| This transgene encodes tau-linked-LacZ under the control of a minimal promoter comprised of 161 base pairs upstream of the Olfr151 transcription start site and 176 base pairs of the 5' nontranslated exon 1. It does not drive expression of any olfactory receptor sequence. It is useful in mapping promoter elements important for olfactory receptor expression and the related olfactory sensory neuron development. Histology shows LacZ activity spread over a large domain of the dorsal aspect of the medial half-bulb, not coalescing into glomeruli. | ||
| 007973 | B6;CBA-Tg(Olfr16-taulacZ)1Mom/MomJ | Cryopreserved - Ready for recovery |
| This transgene encodes tau-linked-LacZ under the control of a minimal promoter comprised of 148 base pairs upstream of the Olfr116 transcription start site and 154 base pairs of the 5' nontranslated exon 1. It does not drive expression of any olfactory receptor sequence. It is useful in mapping promoter elements important for olfactory receptor expression and the related olfactory sensory neuron development. Histology shows LacZ expressing olfactory neurons projecting diffusely to a large domain centrally in the medial half-bulb, not coalescing into glomeruli. | ||
| 007976 | B6;CBA-Tg(Olfr713-taulacZ)4Mom/MomJ | Cryopreserved - Ready for recovery |
| This transgene encodes tau-linked-LacZ under the control of a minimal promoter comprised of 143 base pairs upstream of the Olfr713 transcription start site and 163 base pairs of the 5' nontranslated exon 1. It does not drive expression of any olfactory receptor sequence. It is useful in mapping promoter elements important for olfactory receptor expression and the related olfactory sensory neuron development. Histology shows mosaic expression of LacZ in the ventral main olfactory epithelium. | ||
| 006743 | B6;CBA-Tg(P-taulacZ)11Mom/MomJ | Cryopreserved - Ready for recovery |
| The Tg(P-taulacZ)11Mom transgene is expressed in approximately 10% of olfactory sensory neurons, which are also expected to co-express an endogenous olfactory receptor. This transgene is preferentially expressed in olfactory sensory neurons that co-express class II olfactory receptors, with a 100-fold lower expression found in class I expressing neurons. Immunohistochemistry shows expression in NCAM2 staining glomeruli, which are generally dorsally projecting, and a ventral subset of NQO1 staining glomeruli, which are generally ventrally projecting. Expression is absent in glomeruli in the dorsal-medial and anterior-dorsal olfacotry bulb giving an unlabeled butterfly-shaped pattern in a dorsal view. | ||
| 006793 | B6;CBA-Tg(P-taulacZ)13Mom/MomJ | Cryopreserved - Ready for recovery |
| The Tg(P-taulacZ)13Mom transgene is expressed in approximately 10% of olfactory sensory neurons, which are also expected to co-express an endogenous olfactory receptor. This transgene is preferentially expressed in olfactory sensory neurons that co-express class II olfactory receptors, with a 100-fold lower expression found in class I expressing neurons. Immunohistochemistry shows expression in NCAM2 staining glomeruli, which are generally dorsally projecting, and a ventral subset of NQO1 staining glomeruli, which are generally ventrally projecting. Expression is absent in glomeruli in the dorsal-medial and anterior-dorsal olfacotry bulb giving an unlabeled butterfly-shaped pattern in a dorsal view. | ||
| 006742 | B6;CBA-Tg(P-taulacZ)8Mom/MomJ | Cryopreserved - Ready for recovery |
| The Tg(P-taulacZ)8Mom transgene is expressed in approximately 10% of olfactory sensory neurons, which are also expected to co-express an endogenous olfactory receptor. This transgene is preferentially expressed in olfactory sensory neurons that co-express class II olfactory receptors, with a 100-fold lower expression found in class I expressing neurons. Immunohistochemistry shows expression in NCAM2 staining glomeruli, which are generally dorsally projecting, and a ventral subset of NQO1 staining glomeruli, which are generally ventrally projecting. Expression is absent in glomeruli in the dorsal-medial and anterior-dorsal olfacotry bulb giving an unlabeled butterfly-shaped pattern in a dorsal view. When mice co-express both this transgene and a YFP labelled null allele of Olfr545, a class I olfactory receptor, they label distinct regions; when mice co-express both this transgene and a GFP labelled null allele of Olfr160, a class II olfactory receptor, they label the same reg ..... For more information please see the full phenotype on the strain data sheet | ||
| 004141 | B6;CBA-Tg(UAS-lacZ)65Rth/J | Cryopreserved - Ready for recovery |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Expression of the transgenic lacZ gene is directed by upstream activating sequence (UAS) elements. This strain serves as a reporter for mice employing GAL4/UAS bigenic system for controlled gene expression in the developing CNS. In this system, a transgenic strain expressing the yeast transcriptional activator GAL4 (see Stock No. 003829) is bred to a second transgenic, target strain bearing an UAS-regulated gene. In the double transgenic offspring, an UAS-regulated gene would be selectively expressed in tissues expressing GAL4. | ||
| 005654 | B6C3-Del(16Cbr1-ORF9)1Rhr/J | Cryopreserved - Ready for recovery |
| These mice are monosomic for the mouse chromosome segment MMU16. The deleted segment contains mouse orthologs of 33 conserved and minimally conserved genes in the human Down's syndrome critical region (DSCR). The borders of the deletion are defined by the carbonyl reductase 1 (Cbr1) gene and a site adjacent to the myxovirus (influenza virus) resistance 2 (Mx2) locus. Monosomic mice are viable, fertile and are significantly smaller than wildtype (euploid) littermates from birth to adulthood. This mouse may be useful in studies of Down syndrome and further exploring the ploidy of DSCR/MMU16. | ||
| 007041 | B6Ei.129P2-Nr5a1tm2Klp/EiJ | Cryopreserved - Ready for recovery |
| Mice homozygous for this floxed allele are viable and fertile. These mutant mice have loxP sites flanking the C-terminal coding exon. When bred to Cre-recombinase expressing mice, offspring will have a deletion of this exon in the cre expressing tissue(s). These floxed mice may be useful in studying steroidogenic factors and pituitary gonadotrope function.
For example, when crossed to a strain expressing Cre recombinase in the anterior and intermediate lobes of the pituitary gland (see Stock No. 004426), this mutant mouse strain may be useful in studies of pituitary gonadotrope function. | ||
| 006305 | B6Ei.Cg-Nr0b1tm1.1Lja Tg(Sry)2Ei Chr YAKR/EiJ | Cryopreserved - Ready for recovery |
| 009685 | B6N.129S1(Cg)-Tnkstm1.1Yjc/J | Cryopreserved - Ready for recovery |
| Homozygous (TANK1-/-) mice are viable and fertile with no reported developmental or gross physical abnormalities. As exon 1 is deleted from the targeted allele, no full length protein (TANK1) is detected in thymus, testis or spleen tissues. Because the deletion does not encompass the potential alternative promoter between exons 4-5, an alternative transcript and subsequent low molecular weight protein (TANK1a) is expressed in testis (but not in other assayed tissues). These TANK1-mutant mice may be useful for studying the post-translational modification of proteins associated with cell cycle, mitosis, telomere length maintenance/aging, DNA replication/repair, cellular senescence, apoptosis, tumorigenesis, vesicle trafficking, and insulin responses. These TANK1-mutant mice may also be used along with TANK2-mutant mice (Stock No. 006200). | ||
| 002955 | C.129S7-Gt(ROSA)26Sor/J | Cryopreserved - Ready for recovery |
| Mice hemizygous or homozygous for the ROSA26 retroviral insertion display no distinguishing phenotype. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult mouse. | ||
| 006245 | C.Cg-Tg(SFTPC-rtTA)5Jaw/J | Cryopreserved - Ready for recovery |
| Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human SFTPC, surfactant, pulmonary-associated protein C, promoter. rtTA activity is detected in lung peripheral epithelial cells from adult mice and in embryos from pregnant females treated with the tetracycline analog, doxycycline (dox). In the latter, rtTA-induced expression of a luciferase reporter under the regulation of a tetracycline-responsive promoter (TRE; tetO) has been detected as early as embryonic day 10.5. When hemizygotes are mated to a second transgenic strain carrying a gene of interest under the regulatory control of a TRE, expression of the target gene in the bitransgenic offspring may be regulated by dox; in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. This ..... For more information please see the full phenotype on the strain data sheet | ||
| 006242 | C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for the CCSP-rtTA transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat Scgb1a1, secretoglobin, family 1A, member 1 (uteroglobin), gene promoter. In situ hybridization detects rtTA gene product (mRNA) in bronchial and type II epithelial cells of lung tissue from adult transgenic mice treated with doxycycline for seven days. Induction of transgene expression is detected as early as postconception day 14 when the pregnant female is treated with doxycycline. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtT ..... For more information please see the full phenotype on the strain data sheet | ||
| 009155 | C57BL/6-Cldn6tm1(cre)Dkwu/J | Cryopreserved - Ready for recovery |
| This targeted strain expresses Cre under the control of the claudin 6 promoter. When bred to a floxed reporter strain, broad expression throughout the epiblast is seen at E6.5. By E9.5, expression is restricted to the endoderm-derived structures such as the oral cavity, digestive tract, and lung. In addition, it is expressed in mesonephros and otic vesicle. Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. | ||
| 008314 | C57BL/6-Tg(HBB-cre)12Kpe/J | Cryopreserved - Ready for recovery |
| These transgenic mice express Cre recombinase under the control of the human beta hemoglobin (HBB) promoter and intron 2-enhancer fragment, and the human beta hemoglobin locus control region (LCR). Cre recombinase expression is detected in blood, brain, gonads, spleen and liver by RT-PCR analysis and in blood by RNAse protection assay analysis. During development Cre activity is restricted to erythroid tissues. The Donating Investigator reports that recombination occurs at 50-100% efficiency in erythroid/megakarycytic cell lineages beginning at onset of hematopoiesis at approximately embryonic day 7.5. When crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in erythroid tissues. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain represents an effective tool for generating tissue speci ..... For more information please see the full phenotype on the strain data sheet | ||
| 008870 | C57BL/6-Tg(Hspa2-cre)1Eddy/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for the Hspa2-Cre transgene are viable and fertile, with expression of Cre recombinase directed to the developing male germ cells by the mouse Hspa2 (heat shock protein 2 [Hsp70-2]) promoter. The Hspa2-Cre sequences of the transgene are flanked by Acrv1 (acrosomal vesicle protein 1 [SP-10]) insulator elements (which are believed to tether the Acrv1 gene to the nuclear matrix in somatic cells [preventing transcription]). When these transgenic mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination is expected to result in deletion of the floxed sequences in leptotene/zygotene spermatocytes of the male offspring. The donating investigator reports that expression of Hspa2-Cre may be "leaky" and recombination of loxP-flanked sequences can occur in some cells in somatic tissues. These Hspa2-Cre mice may be useful to generate conditional mutations for studying mammalian spermatocyte development, g ..... For more information please see the full phenotype on the strain data sheet | ||
| 002063 | C57BL/6JEi-Tg(Sry)1Ei Chr YAKR/J/EiJ | Cryopreserved - Ready for recovery |
| In this strain, XX mice that carry the Tg(Sry)1Ei transgene are sex-reversed males. XYAKR/J mice heterozygous for TOrl on a C57BL/6J background develop as sex reversed females, but in the presence of the Tg(Sry)1Ei transgene these XY mice develop as normal males with testes. | ||
| 002708 | C57BL/6JEi-Tg(Sry)2Ei Chr YAKR/J/EiJ | Cryopreserved - Ready for recovery |
| In this strain, XX mice that carry the Tg(Sry)2Ei transgene are sex-reversed males. | ||
| 008337 | CBA/Ca-Tg(H2-K-HLA-G,B2M)1Alm/CmwJ | Cryopreserved - Ready for recovery |
| HLA-G transgenic (HLA-Gtg) mice are viable, fertile, and harbor two co-injected transgenes; H-2Kb/HLA-G and hβ2m. It was found that co-injecting the hβ2m transgene greatly facilitated expression of the whole HLA-G molecule, implying that endogenous mouse β2m cannot substitute for its human homolog. In addition, the CBA/Ca genetic background has a spontaneous deletion of the genes that encode the structurally/functionally homologous mouse Qa-2 protein, thus experimental results obtained using these HLA-Gtg mice may be attributed directly to transgene expression. Expression of HLA-G expression is observed in pre-implantation embryos and a variety of tissues; expression in the reproductive organs (uterus, ovary, and testes), lung, and liver is much more similar to mouse expression of Qa-2 than to Kb (despite the presence of the H-2Kb promoter and downstream coding sequence), while HLA-G expression in spleen, ..... For more information please see the full phenotype on the strain data sheet | ||
| 007898 | CBy.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this ROSA26-EGFP BAC transgene are viable and fertile, with widespread EGFP expression in all organs and tissues analyzed. In addition, stable and uniform EGFP expression is observed in differentiated progeny of transplanted hematopoietic stem cells (HSC) isolated from ROSA26-EGFP mice. These ROSA26-EGFP BAC transgenic mice may be useful for fluorescent labeling of cells and tissues both in vivo and in vitro, are suitable for use in histological preparations, and are appropriate as a source of fluorescently labeled donor cell populations (such as tracking hematopoietic cell fate in transplant recipients).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as publish ..... | ||
| 010548 | D1.FVB(Cg)-Tg(CAG-luc,-GFP)L2G85Chco/FathJ | Cryopreserved - Ready for recovery |
| These L2G85.DBA/1 mice harbor the CAG-luc-eGFP L2G85 transgene. Homozygous mice are viable and fertile, with widespread expression of firefly luciferase and enhanced green fluorescent protein directed by the CAG promoter (human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter). Bioluminesence is detected in heart, spleen, muscle, pancreas, skin, thymus and bone marrow. Luciferase activity is not detected in mature erythrocytes, although low levels are detected in erythrocyte precursors and varying levels of activity in all leukocyte subsets tested. Homozygotes have no reported gross physical or behavioral abnormalities. Following luciferin injection, luciferase expression is generally greater in males than females. GFP fluorescence is detected in skin (upper epidermal layers) by fluorescence microscopy. The Donating Investigator reports that for the FVB-Tg(CAG-luc,-GFP)L2G85Chco/J (STOCK#8450) strain, no GFP fluorescence ..... For more information please see the full phenotype on the strain data sheet | ||
| 007042 | D2.129P2(B6)-Nr5a1tm2Klp/EiJ | Cryopreserved - Ready for recovery |
| Mice homozygous for this floxed allele are viable and fertile. These mutant mice have loxP sites flanking the C-terminal coding exon. When bred to Cre-recombinase expressing mice, offspring will have a deletion of this exon in the cre expressing tissue(s). These floxed mice may be useful in studying steroidogenic factors and pituitary gonadotrope function.
For example, when crossed to a strain expressing Cre recombinase in the anterior and intermediate lobes of the pituitary gland (see Stock No. 004426), this mutant mouse strain may be useful in studies of pituitary gonadotrope function. | ||
| 006768 | D2.Cg-Tg(Myh6-Zfpm2)1Sho/EiJ | Cryopreserved - Ready for recovery |
| 011034 | FVB(Cg)-Tg(Ghrhr-cre)3242Lsk/J | Cryopreserved - Ready for recovery |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Transgenic mice express Cre recombinase under the control of the rat growth hormone releasing hormone receptor (Ghrhr). Cre recombinase expression is detected in the anterior pituitary as early as E13.5 and specifically in cells of the Pou1f1(Pit1) lineage, somatotrophs, lactotrophs and thyrotrophs. Expression also is found in vibrissae, part of the eyelid and ear, and in hair follicles of the proximal limb, but not in other tissues tested. When crossed with a strain containing loxP site flanked sequences, Cre-mediated recombination results in deletion of flanked sequences in the offspring. | ||
| 005710 | FVB.129S-Mmp13tm1Werb/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for this loxP-flanked ("floxed") allele are viable and fertile with normal endogenous gene function. Cre-mediated recombination results in replacement of exons 3-5 of targeted gene with a single loxP site. When bred to cre-expressing transgenic strains, these floxed mutant mice may be used to generate whole mouse or tissue-specific targeted mutants that may be useful in examining extracellular matrix remodeling and bone development. Of note: when these floxed mice are bred to mice containing a beta-actin promoter-driven cre transgene the resulting cre-positive, homozygous-null mice show robust accumulation of cartilage matrix caused by a transient expansion of the hypertrophic zone and increased trabecular bone mass that persists for months. | ||
| 006867 | FVB.B6-Ins2Akita/MlnJ | Cryopreserved - Ready for recovery |
| FVB/NJ mice heterozygous for the Akita spontaneous mutation are viable and fertile. The donating investigator reports that the symptoms in heterozygous mutant mice are more severe than those observed in C57BL/6-Ins2Akita mice (Stock No. 003548). These symptoms include hyperglycemia (females > 600mg/dl, males ~560 mg/dl), hypoinsulinemia, polydipsia, and polyuria, beginning at approximately 3-4 weeks of age. In contrast to Akita heterozygotes on a C57BL/6 background, FVB/NJ adult heterozygous females are more hyperglycemic than heterozygous males. Obesity and insulitis do not accompany diabetes. Ins2 is expressed in the fetal yolk sac and is maternally imprinted. Heterozygous mutant females become more hyperglycemic during pregnancy, and are susceptible to embryo malformations leading to reabsorption, even with insulin therapy. Heterozygous mutant males do not produce mutant and wild-type o ..... For more information please see the full phenotype on the strain data sheet | ||
| 006225 | FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J | Cryopreserved - Ready for recovery |
| Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator reports that homozygous transgenic mice are not viable. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human SFTPC, surfactant, pulmonary-associated protein C, promoter. rtTA activity is detected in lung peripheral epithelial cells from adult mice and in embryos from pregnant females treated with the tetracycline analog doxycycline (dox). In the latter, rtTA-induced expression of a luciferase reporter under the regulation of a tetracycline-responsive promoter (TRE; tetO) has been detected as early as embryonic day 10.5. When hemizygotes are mated to a second transgenic strain carrying a gene of interest under the regulatory control of a TRE, expression of the target gene in the bitransgenic offspring can be regulated by dox; in the presence of dox, tra ..... For more information please see the full phenotype on the strain data sheet | ||
| 006222 | FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for the CCSP-rtTA transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat Scgb1a1, secretoglobin, family 1A, member 1 (uteroglobin), gene promoter. rtTA activity is detected in bronchial and type II epithelial cells of lung tissue from adult transgenic mice and in embryos from pregnant females treated with the tetracycline analog doxycycline (dox). In the latter, rtTA-induced expression of a luciferase reporter under the regulation of a tetracycline-responsive promoter (TRE; tetO) has been detected as early as embryonic day 12.5. When hemizygotes are mated to a second transgenic strain carrying a gene of interest under the regulatory control of a TRE, expression of the target gene in the bitransgenic offspring can be regulated by dox; in the presence of dox, transcription of the targ ..... For more information please see the full phenotype on the strain data sheet | ||
| 007483 | FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ | Cryopreserved - Ready for recovery |
| On an albino background the X-linked transgene Tg(Tyr)3412ARpw permits visual identification of gender as early as embryonic day 10.5. This strain is segregating for Tg(Tyr)3412ARpw and homozygous for Tyrc-2J so the individuals not carrying Tg(Tyr3412)ARpw are albino. Because Tg(Tyr)3412ARpw inserted into the X Chromosome, breeding a carrier male with a noncarrier (wild-type) female results in embryos in which all XX individuals develop eye pigment, due to the Tg(Tyr)3412ARpw inherited from their father, while all XY individuals have non-pigmented eyes, having inherited a wild-type X Chromosome from their mother.
This strain is also homozygous for Tg(SryEGFP)92Ei. This reporter transgene consists of a 5' regulatory segment of the Sry gene driving EGFP. This transgene is expressed in the pre-support cell lineage (pre-sertoli and pre-granulosa cells) of the fetal genital ridge (Albrecht and Eicher, 2001) and in discrete areas the adult male but not female ..... | ||
| 007800 | FVB/N-Tg(Ins1-luc)VUPwrs/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this MIP-luc transgene are viable and fertile, with luciferase (luc) expression under control of the mouse insulin I promoter (MIP or Ins1). After injection of the luciferin substrate, luc expression can be visualized using bioluminescence imaging (BLI). Mice from founder line VU (MIP-Luc-VU) exhibit strong and consistent luc bioluminescence emanating exclusively from β cells of the pancreatic islet, and luc-expressing beta cells can be visualized through the skin. MIP-Luc-VU pancreatic islets have normal islet architecture and insulin secretion both in vivo and in vitro, with luciferase intensity reporting the number of islets. In transplantation settings and models of increased/decreased beta cell mass, bioluminescence is proportional to beta cell mass (incorporating some aspects of islet function, with signal up-regulation in hyperglycemic states). These MIP-Luc-VU mice may be useful as a source of luciferase-expressing β cel ..... For more information please see the full phenotype on the strain data sheet | ||
| 004066 | FVB;129S-Men1tm1.1Ctre/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous null for the Men1 gene die in utero at embryonic days 10.5-11.5, exhibiting delayed development often (20%) with defects in cranial/facial formation. At birth, heterozygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. At nine months, ~80% of the heterozygous-null mice develop abnormalities in pancreatic islet cells, the severity of which ranges from hyperplasia to insulin-producing tumors. Parathyroid adenomas are also observed at this age. Tumor incidence is progressive, with occurrences in multiple endocrine tissues (pancreatic islets, parathyroids, thyroid, adrenal cortex, pituitary) by sixteen months of age. | ||
| 006001 | STOCK Dicer1tm1Bdh/J | Cryopreserved - Ready for recovery |
| These mice contain loxP sites on either side of exon 23 of the targeted gene. Mice homozygous for this "Dicer-flox" allele are viable and fertile and exhibit no gross phenotypic or behavioral abnormalities. Expression of the targeted allele is indistinguishable from wildtype despite the presence of an frt-flanked neomycin cassette between exon 23 and the 3' loxP site. Cre-mediated recombination (resulting in deletion of exon 23) in the germline leads to developmental arrest at embryonic day 7.5 (E7.5). Tissue specific deletion results in the loss of microRNA (miRNA) processing. Mutant mice can be used to generate cell/tissue-specific deletions of the endogenous gene for applications in embryonic development, translation, protein processing and miRNA/siRNA regulation of gene expression.
For example, when crossed to a strain expressing Cre recombinase in mesenchyme (see Stock No. 005584), this mutant mouse s ..... | ||
| 005134 | STOCK Disp1tm2Amc/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele, which is hypomorphic. | ||
| 009063 | STOCK Ednrbtm1Nrd/J | Cryopreserved - Ready for recovery |
| Mice heterozygous for this Ednrbflex3 allele are viable and fertile, with a loxP-flanked neo cassette upstream of exon 3, as well as a loxP site downstream of exon 3 of the Ednrb (endothelin receptor type B or ET-B receptor) gene. When bred to mice that express Cre recombinase, the resulting offspring can have one of three resulting genotypes in the cre-expressing tissue(s); only the neo selection cassette deleted, only exon 3 deleted, or both the neo selection cassette and exon 3 deleted. The two latter genotypes are expected to result in a frameshifted transcript that is reported to confer the null phenotype. These mutant mice may be useful in generating conditional mutations for studying the role of Ednrb in development of melanocytes, development of neurons and glia of the enteric nervous system, neural crest-derived cells, mesenchymal-derived smooth muscle cells, vasodilation, mitogen signaling and cancer, and human Hirschsprung's dis ..... For more information please see the full phenotype on the strain data sheet | ||
| 009672 | STOCK Egln1tm1Kael/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene (widespread deletion of expression is embryonic lethal). This strain may be useful in studies of development and cardiovascular disease.
For example, when crossed to a strain expressing tamoxifen-inducible Cre recombinase in most tissues (see Stock No. 004682), this mutant mouse strain may be useful in studies of polycythemia and congestive heart failure. | ||
| 007916 | STOCK En1tm2(cre)Wrst/J | Cryopreserved - Ready for recovery |
| En1Cki (or En1Cre) mutant mice harbor a Cre recombinase (cre) "knock-in" allele that also abolishes endogenous gene function. While heterozygotes are viable and fertile, homozygous mice die at birth. Under control of the upstream promoter/enhancer elements, cre expression is observed in a pattern consistent with the wild-type gene. When heterozygotes are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequences in En1-expressing tissues of the offspring. These En1Cki (or En1Cre) mice may be useful for Cre-lox applications studying engrailed protein function such as deleting genes in spinal cord V1 interneurons, the embryonic mesencephalon and rhombomere 1 by E9, as well as in the ventral ectoderm of the limbs, in a subset of somite cells, and some mesoderm-derived tissues.
Of note, these mice may also be useful in conjunction with o ..... | ||
| 007912 | STOCK En1tm2Alj/J | Cryopreserved - Ready for recovery |
| En-1lki (or En1lacZ) mutant mice harbor a β-galactosidase "knock-in" allele that also abolishes endogenous gene function. While heterozygotes are viable and fertile, homozygous mice die at birth (unless when maintained on a C57BL/6 genetic background). Under control of the upstream promoter/enhancer elements, lacZ expression is observed in a pattern almost identical to the wildtype gene. These En-1lacZ mice may be useful for reporter protein expression in En1-expressing tissues in studying engrailed protein function in limb patterning along the dorso-ventral axis, spinal cord V1 interneuron development, embryonic mesencephalon and rhombomere 1 (cerebellum) development, as well as developing somites, and skin. Of note, these mice may also be useful in conjunction with other engrailed mutants (such as Stock No. 007916, Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 007917 | STOCK En1tm7(cre/ESR1)Alj/J | Cryopreserved - Ready for recovery |
| While mice heterozygous for this En1-CreERT1 mutation are viable and fertile, homozygotes die at birth. Because the Cre-ERT1 fusion gene is inserted between the transcriptional start site and the first exon of the engrailed 1 (En1) gene, tamoxifen-inducible cre activity is controlled by the endogenous En1 regulatory elements. The Cre-ERT1 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT1 can only gain access to the nuclear compartment after exposure to OHT. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these En1-CreERT1 mice are bred with ..... For more information please see the full phenotype on the strain data sheet | ||
| 007918 | STOCK En1tm8.1Alj/J | Cryopreserved - Ready for recovery |
| Mice homozygous for the En1flox conditional allele are viable and fertile, with loxP sites flanking the coding region of exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (encoding the engrailed homeodomain) deleted in the cre-expressing tissue(s). These En1flox mice may be useful in generating conditional mutations for studying engrailed protein function in the embryonic mesencephalon and rhombomere 1, as well as developing cerebellum, limbs, somites, and skin. Of note, these mice may also be useful in conjunction with other engrailed mutants (such as Stock No. 007912, Stock No. 007916, Stock No. 007917, Stock No. 007924, and Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 007924 | STOCK En2tm4(cre/ERT2)Alj/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this En2-CreERT2 mutation are viable but may not breed well (reported as "semi-fertile"). As the Cre-ERT2 fusion gene is inserted into the 5' UTR of the engrailed 2 (En2) locus, tamoxifen-inducible cre activity is controlled by the endogenous En2 regulatory elements. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to OHT. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these En2-CreERT2 mice are bred with mice containing a loxP-flanked se ..... For more information please see the full phenotype on the strain data sheet | ||
| 007925 | STOCK En2tm5.1Alj/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this En2flox-taulacZ (also called En2-floxLacZ, En2tau-lacZ, or En2-tau-lacZ) mutation are viable but may not breed well (reported as "semi-fertile"). These mice harbor a loxP-flanked tau β-galactosidase "knock-in" allele that also abolishes engrailed 2 (En2) gene function. While both the tau-lacZ and En2 transcripts are made, only tau-lacZ is translated to protein. Expression of lacZ mimics that of the endogenous En2 gene. When bred to mice that express Cre recombinase, the resulting offspring will have the lacZ reporter deleted in the cre-expressing tissue(s) with subsequent restoration of En2 translation. These En2flox-taulacZ mutant mice may be useful for conditional reporter protein expression in En2-expressing tissues (including the early mesencephalon and rhombomere 1, and developing cerebellum and jaw muscles), as well as for conditional restoration of ..... For more information please see the full phenotype on the strain data sheet | ||
| 007674 | STOCK Esrrbtm1.1Nat/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this Nr3b2CKO allele possess loxP sites flanking exon 2 of the targeted gene and are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the exon containing the initiator methionine codon and encoding the N-terminal 132 amino acids (including part of the DNA-binding domain) deleted in the cre-expressing tissue(s). Of note, if the conditional Nr3b2CKO is deleted by Cre recombinase in the placenta and embryo, embryonic lethality will result (placental defect). If the conditional Nr3b2CKO is deleted by Cre recombinase only in the embryo, the resulting mice exhibit an inner ear defect (decreased endolymph production) resulting in deafness and defective balance. These Nr3b2CKO mutant mice may be useful in generating conditional mutations to study disorders of hearing and balance, inner ear development (such as endolymph-producing epithelia withi ..... For more information please see the full phenotype on the strain data sheet | ||
| 008196 | STOCK Gata6tm2.1Sad/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this Gata6loxp conditional allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for the majority of the GATA6 protein) deleted in the cre-expressing tissue(s). These Gata6loxp mice may be useful in generating conditional GATA binding protein 6 mutations for studying GATA6 function in organogenesis or in adult mice.
For example, when bred to a strain expressing Cre recombinase in the villi and crypt cells of the intestine (see Stock No. 004586 for example), or when bred to a strain expressing Cre recombinase in the embryo (see Stock No. 003724, 003314 for example), this mutant mouse strain may be useful in studies of the transcrip ..... | ||
| 007927 | STOCK Gbx2tm1.1Alj/J | Cryopreserved - Ready for recovery |
| Mice homozygous for the Gbx2flox conditional allele are viable and fertile, with loxP sites flanking the protein coding region of exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (encoding the gastrulation brain homeobox 2 homeodomain) deleted in the cre-expressing tissue(s). These Gbx2flox mice may be useful in generating conditional mutations for studying gastrulation brain homeobox protein function in the embryonic mesencephalon and rhombomere 1, as well as developing cerebellum and thallamus. | ||
| 005994 | STOCK Mbtps1tm1Jdh/J | Cryopreserved - Ready for recovery |
| These mice carry a targeted mutation in which exon 2 of the targeted gene is flanked by loxP sites. A loxP-flanked ("floxed") neomycin resistance cassette also is inserted downstream in intron 2. Homozygotes are viable and fertile, and the floxed gene appears to function normally. When homozygotes are crossed with transgenic strains expressing Cre-recombinase, cre-mediated recombination of the loxP-flanked sequences can result in one of three genotypes: a) deletion of the neo cassette only, leaving a loxP-flanked second exon and unimpaired endogenous gene function. b) Deletion of exon 2 only, leaving a loxP-flanked neo cassette and no endogenous gene function. c) Deletion of both the neo cassette and exon 2, leaving a single loxP site and no endogenous gene function. When these floxed mutant mice are bred to mice carrying the Mx1-cre transgene (for example, Stock No. 003556), liver- ..... For more information please see the full phenotype on the strain data sheet | ||
| 010928 | STOCK Mnx1tm1Spf/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon3 of the Mnx1 (motor neuron and pancreas homeobox 1) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene, exon 3 is deleted in cre-expressing tissues in the offspring. This strain may be useful in studies of neurodevelopment. | ||
| 006951 | STOCK Notch1tm2Rko/GridJ | Cryopreserved - Ready for recovery |
| Mice homozygous for this "floxed" Notch1 allele (fN1) are viable and fertile. In the targeted allele, loxP sites were placed flanking exon 1 of the targeted gene. When these floxed mice are bred to mice expressing Cre recombinase, exon 1 of the targeted gene is deleted in cre-expressing tissue(s) in the cre-positive, homozygous floxed offspring. These conditional knockout mice may be useful in generating tissue-specific mutants for studying the development of a wide range of tissues: for example, when crossed to a strain expressing Cre recombinase primarily in the nervous system (see Stock No. 003771), this mutant strain may be useful in studies of apoptosis in neural development.
When crossed to a strain expressing a differential Cre mediated reporter protein labeling: Notch1 signaling in actively cycling stem/progenitor cells (see Stock No. 006953 ..... | ||
| 006953 | STOCK Notch1tm3(cre)Rko/J | Cryopreserved - Ready for recovery |
| While homozygotes die at embryonic day 9.5 (E9.5), mice heterozygous for this N1::cre allele are viable and fertile. Mutant mice express a Notch1-Cre fusion protein formed by the insertion of Cre recombinase into the Notch1 locus replacing the intracellular domain (NICD1). As such, endogenous function of the NICD1 is terminated. Interaction of N1::cre fusion receptors in vivo with Notch-DSL (-Delta, Serrate and Lag-2) ligands results in proteolytic cleavage of the transmembrane domain tether and subsequent release of Cre recombinase protein from the plasma membrane. When bred to mice with a loxP-flanked reporter sequence, cell descendants have differential Cre-mediated reporter protein labeling; Notch1 signaling in actively cycling stem/progenitor cells will mark all their descendants producing a "clone", whereas Notch1 activation in transit amplifying or differentiating cells will result in small clones (2-4 cells) or in salt-and-pepper patterns of individually la ..... For more information please see the full phenotype on the strain data sheet | ||
| 006702 | STOCK Ntstm1Mom/MomJ | Cryopreserved - Ready for recovery |
| Through bicistronic expression of enhanced green fluorescing protein and neurotensin, this strain permits the visualization of the mitral and tufted cells of the main olfactory bulb thereby permitting developmental analysis in the lateral olfactory tract. | ||
| 005384 | STOCK Numbtm1Zili Numbltm1Zili/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 1 of the Numb gene and loxP sites flanking exons 1 through 3 of the Numbl gene. Mice that are homozygous for these alleles are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.
For example, when crossed to a strain expressing an interferon inducible Cre recombinase (see Stock No. 003556), this mutant mouse strain provides information about hemopoiesis and lymphopoesis. | ||
| 009061 | STOCK Osr1tm1(EGFP/cre/ERT2)Amc/J | Cryopreserved - Ready for recovery |
| Homozygous mice die upon birth with multiple abnormalities of the heart and urogenital system (among other tissues). Heterozygous mice are viable and fertile. The Osr1eGFPCreERt2 (Osr1GCE) "knock-in" allele both abolishes Osr1 gene function and expresses an eGFPCreERt2 fusion protein (EGFP and creERT2 fusion protein from the Osr1 promoter/enhancer elements). While EGFP immunofluorescence is observed in intermediate mesoderm and dorsal lateral plate mesoderm of developing embryos (~E8.5), Cre-ERT2 fusion gene activity is inducible; observed in the same cells only following tamoxifen administration. As such, when Osr1GCE mice are bred with mice containing loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Osr1-expressing cells of the offspring. Of note, removal of the frt-flanked neo cassette is reported to result in ectopic Cre recomb ..... For more information please see the full phenotype on the strain data sheet | ||
| 010499 | STOCK Pygo2tm1.1Ssp/J | Cryopreserved - Ready for recovery |
| Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Excision of the floxed fragment in eye tissues results in microophthalmia and abnormal lens induction. This mutant mouse strain is useful in studies of lens development, but may also be useful in studies of other organs as well.
For example, when crossed to a strain with widespread Cre recombinase expression (see Stock No. 003465 and 006054), this mutant mouse strain may be useful in lens development. When crossed to a strain expressing Cre recombinase in midbrain and spinal cord (see Stock No. 007807), this mutant mouse strain may b ..... | ||
| 006473 | STOCK Smyd1tm1Dsr/J | Cryopreserved - Ready for recovery |
| Mice heterozygous for this targeted mutation are viable and fertile. Mice homozygous for this targeted allele, however, die between embryonic day (E) 9.5 and 10.5 due to cardiomyocyte maturation defects, including an enlarged heart, single left-side ventricular chamber with tremendous extra cellular matrix expansion between the myocardial and endocardial layers, and defective Hand2 expression. No mRNA transcripts from the targeted mutant gene are detected in cardiac tissue from E9.5 homozygotes. These mutant mice may be useful in studying cardiomyocyte differentiation and cardiac morphogenesis. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available. | ||
| 008469 | STOCK Wnt9btm1.2Amc/J | Cryopreserved - Ready for recovery |
| Mice homozygous for the Wnt9bc allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). Such deletion is predicted to result in out-of-frame splicing of exon 1 to exon 3, and consequently a mutant transcript that would encode a nonfunctional peptide comprising the first 27 amino acids of Wnt9b that includes the signal peptide. These Wnt9bc mice may be useful in generating conditional mutations for studying the role of Wnt9b (and other Wnt family members) in development and canonical Wnt signaling cascades, including metanephric kidney and urogenital system development. | ||
| 005438 | STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J | Cryopreserved - Ready for recovery |
| While mice hemizygous for this Z/RED transgene are reported to be viable and fertile, it has been our experience at The Jackson Laboratory that hemizygous animals are often smaller than littermates and subject to postnatal mortality. Delayed weaning greatly enhances the survival. Although homozygous animals are born, animals have not survived past five weeks of age. These transgenic mice express beta-galactosidase under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with red fluorescent protein (DsRed*MST) expression in tissues expressing Cre recombinase. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells. | ||
| 006850 | STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this Cre-conditional IC-Notch (or Z/EG-Notch) transgene are viable and fertile. Homozygotes die in utero, presumably due to high lacZ expression. Prior to Cre-mediated excision of the "floxed" STOP sequence, high expression of lacZ is observed in cells and tissues. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the intracellular human NOTCH1 (IC-Notch) and EGFP to proceed in the Cre recombinase expressing cells. For example, endothelial expression of IC-Notch using different Cre-transgenic matings is associated with neural, somite and angiogenic defects, infertility in females, and embryonic lethality in the resulting offspring. These transgenic mice may be useful for global expression of lacZ or, when crossed to a Cre recombinase expressing strain, for studying the role of Notch signaling during both embryonic develo ..... For more information please see the full phenotype on the strain data sheet | ||
| 006613 | STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J | Cryopreserved - Ready for recovery |
| Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) gene, Tle1 (also known as Groucho-related gene 1; Grg1) under the regulation of the chicken beta actin promoter (ACTB) and the cytomegalovirus (CMV) intermediate-early enhancer. An internal ribosome entry sequence (IRES) followed by the human alkaline phosphatase (ALPP) reporter gene inserted downstream of the Tle1 gene directs co-expression of ALPP and TLE1. TLE1 and ALPP co-expression is blocked, however, by the presence of a loxP-flanked Bgeo (beta-galactosidase/neomycin resistance fusion protein) cassette inserted between the ACTB/CMV promoter and the Tle1 coding sequence. In the absence of Cre recombinase, beta-galactosidase activity is detected in all tissues tested including pancreas, lung, kidney, cerebellum, heart, m ..... For more information please see the full phenotype on the strain data sheet | ||
| 003773 | STOCK Tg(CAG-ECFP)CK6Nagy/J | Cryopreserved - Ready for recovery |
| Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Notable exceptions to this phenotype are erythrocytes and adipocytes in which fluorescence is negligible or absent. | ||
| 003115 | STOCK Tg(CAG-EGFP)B5Nagy/J | Cryopreserved - Ready for recovery |
| This transgenic strain expresses an Enhanced Green Fluorescent Protein (Clontech) driven by chicken beta-actin promoter and CMV intermediate early enhancer. Mice, and cells derived from them, are distinguished from wildtype on the basis of fluorescence. The transgene is expressed in all nucleated embryonic tissues. Cells and tissues with increased hemoglobin content exhibit reduced fluorescence as development progresses. In newborn and adult mice, the entire organ system expresses EGFP. Though widespread, expression levels vary between different organs. This strain can be used as a source of fluorescently marked cells or tissues. | ||
| 013754 | STOCK Tg(CAG-KikGR)75Hadj/J | Cryopreserved - Ready for recovery |
| CAG::KikGR75 transgenic mice express a Kikume Green-Red (KikGR) photoconvertible fluorescent protein under the control of a CMV enhancer/chicken beta-actin promoter (CAGGS) promoter. Mice homozygous for the transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. KikGR, engineered from Favia favus coral, changes color from green to red upon activation in embryos, adult mice, and embryonic stem (ES) cells. At basal state, green fluorescence is seen in all cells. A single cell or group of cells, exposed to 405 nm wavelength light, undergo photo conversion and fluoresce red. Since KikGR is developmentally neutral and non-toxic, the movement of these fluorescent cells, and their progeny, can be imaged during embryonic development. Mice from founder line 75 exhibit KikGR expression in a widespread mosaic pattern allowing for analysis of cell morphology and cell behavior dynamics, while mice from founder lin ..... For more information please see the full phenotype on the strain data sheet | ||
| 011107 | STOCK Tg(CAG-Venus)1Hadj/J | Cryopreserved - Ready for recovery |
| Mice harboring the lipid modified CAG-YFP* transgene are viable and fertile, normal in size and do not display any gross physical or behavioral abnormalities. The CAG promoter directs widespread expression of a myristoyl-Venus yellow fluorescent protein (YFP) fusion throughout embryonic development and adulthood. Expression is targeted to the plasma membrane and, in particular, the perinuclear regions and vesicular structures located inside the nucleus. In addition, expression is found in the endosomes of the secretory pathway and the Golgi. Hemizygotes and homozygotes exhibit identical distribution patterns, however, homozygotes exhibit increased fluorescence. This mutant mouse strain may be useful for in vivo imaging of cell morphology, plasma membrane dynamics and lipid localization. | ||
| 005645 | STOCK Tg(CAG-mRFP1)1F1Hadj/J | Cryopreserved - Ready for recovery |
| Mice homozygous for the transgene exhibit robust and widespread expression of monomeric red fluorescent protein-1 (mRFP1) in embryonic stem cells, embryos, and adults. Unlike tetrameric or dimeric fluorescent proteins, high levels of mRFP1 expression do not affect cell morphology, developmental potential, viability, and fertility of homozygous mice. Various optical imaging modalities can be used to visualize mRFP1 expressing cells in culture, in embryos and adult mice. Cells from transgenic mRFP1 mice, sampled along with green and cyan fluorescent cells from other mice, show clearly discernable fluorescence. This mouse may be useful in studies of mRFP1 cell lines, transplantation, embryo chimera experiments, and fluorescent imaging and technology development. | ||
| 001788 | STOCK Tg(Fabp1-GH1)10Bir/J | Cryopreserved - Ready for recovery |
| 001400 | STOCK Tg(Fabp1-GH1)5Bir/J | Cryopreserved - Ready for recovery |
| 001515 | STOCK Tg(Fabp1-GH1)7Bir/J | Cryopreserved - Ready for recovery |
| 003528 | STOCK Tg(GFAP-TVA)5Hev/J | Cryopreserved - Ready for recovery |
| This strain expresses the avian cell surface receptor(TVA) for subgroup A avian leukosis viruses. The transgene is under the control of an astrocyte-specific promoter derived from the human glial fibrillary acidic protein (GFAP ) gene. The result is to render glial cells specifically susceptible to infection by viral vectors making possible the glia-specific viral transfer of genes. | ||
| 007896 | STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this ROSA26-EGFP BAC transgene are viable and fertile, with widespread EGFP expression in all organs and tissues analyzed. In addition, stable and uniform EGFP expression is observed in differentiated progeny of transplanted hematopoietic stem cells (HSC) isolated from ROSA26-EGFP mice. These ROSA26-EGFP BAC transgenic mice may be useful for fluorescent labeling of cells and tissues both in vivo and in vitro, are suitable for use in histological preparations, and are appropriate as a source of fluorescently labeled donor cell populations (such as tracking hematopoietic cell fate in transplant recipients). | ||
| 001878 | STOCK Tg(IFABP-GH)11Bir/J | Cryopreserved - Ready for recovery |
| 001879 | STOCK Tg(IFABP-GH)12Bir/J | Cryopreserved - Ready for recovery |
| 001909 | STOCK Tg(IFABP-GH)15Bir/J | Cryopreserved - Ready for recovery |
| Obligate hemizygous F1 offspring from founder 54 carried approximately 60 copies of the transgene in tandem head-to-tail arrangement in a single insertion site. Expression of the reporter gene, human growth hormone, is appropriately restricted to the intestine where the duodenum and proximal jejunum show expression levels normal for intestinal fatty acid binding protein, but the distal small intestine shows reduced expression. Resultant serum human growth hormone levels were 6-38 ng/ml which is approximately 1000 times lower than in mice carrying a transgene with an extended I-FABP promoter which includes nucleotides -1178 to +28. Use of the extended (-1178 to +28) promoter also results in a more normal expression in the distal small intestine. (Sweetser et al., 1988.) | ||
| 008122 | STOCK Tg(Ins2-cre/ERT)1Dam/J | Cryopreserved - Ready for recovery |
| These RIP-CreER transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the rat insulin 2, Ins2, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in islet cells of the pancreas. Hemizygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted ..... For more information please see the full phenotype on the strain data sheet | ||
| 003529 | STOCK Tg(NES-TVA)J12Ech/J | Cryopreserved - Ready for recovery |
| This strain expresses the avian cell surface receptor(TVA) for subgroup A avian leukosis viruses. The transgene is under the control of a glial progenitor-specific promoter derived from the human nestin (NES) gene. The result is to render glial progenitor cells specifically susceptible to infection by viral vectors making possible the glia-specific viral transfer of genes. Please note: Mice recovered from this cryopreserved colony may have retinal degeneration. | ||
| 009102 | STOCK Tg(Nefh-cre)12Kul/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this mNF-H-cre transgene are viable and fertile, with nuclear localized-Cre recombinase expression directed to neurons, coinciding with late stages of brain development, by the mouse neurofilament-H gene (Nefh) promoter. The cre expression in neurons (but not astrocytes) of the brain and spinal cord is mosaic with highest expression in the cortex and hippocampus. Cre recombinase activity begins around embryonic day (E)16.5-E18.5, with significantly robust activity observed after one week of age. The donating investigator reports that mNFHcre mouse line 12 homozygotes develop a neurological phenotype with seizures and become infertile and moribund. When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequences in the resulting offspring. | ||
| 002858 | STOCK Tg(Nes-cre)1Wme/J | Cryopreserved - Ready for recovery |
| These transgenic mice carry the Cre recombinase gene under the control of the rat nestin promoter. When crossed with a strain carrying a gene flanked by loxP sites, partial deletion of the loxP-flanked allele occurs before embryonic day 10.5 and is detectable in all adult organs examined including germ line cells. Because of the partial deletion, these balancer1 cre transgenic mice, as well as the balancer2 cre transgenic mice (Stock No. 002859), may be used to generate mosaic mice consisting of cells that are either wildtype or mutant for the gene of interest. The proportion of cells that carry the mutant allele varies between tissues and between individual mice. In cases where deletion of a particular gene is lethal, the generation of mosaic mice using these strains can bypass lethality in some mosaic individuals. The balancer2 line induces more variable deletion of loxP-flanked genes than the ..... For more information please see the full phenotype on the strain data sheet | ||
| 002859 | STOCK Tg(Nes-cre)2Wme/J | Cryopreserved - Ready for recovery |
| These transgenic mice carry the Cre recombinase gene under the control of the rat nestin promoter. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing cre, generating a proportion of mosaic animals. All adult organs, including germ-line cells, may be affected. The balancer2 line of transgenic mice show greater variability and instability in their generation of mosaics than the balancer1 line (Stock No. 002858). This variability of the balancer2 line can be of particular benefit if the gene of interest is vital and mosaic individuals are used to bypass lethality. | ||
| 012859 | STOCK Tg(Neurog1-cre)1Jejo/J | Cryopreserved - Ready for recovery |
| These transgenic mice express Cre recombinase under the control of the mouse Neurog1, neurogenin, promoter. When crossed with a reporter strain, cre activity mimics the endogenous gene expression patterns. in specific populations of interneurons and some motor neurons in the neural tube between embryonic days 10-13. Also, Cre recombination occurs in Neurog1 lineages that include subpopulations of neurons in the olfactory bulb, hippocampus, cortex, thalamus, midbrain, cerebellum, and dorsal root and cranial ganglia. It is not known if homozygotes are viable. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. | ||
| 008119 | STOCK Tg(Neurog3-cre/Esr1*)1Dam/J | Cryopreserved - Ready for recovery |
| These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse neurogenin 3, Neurog3, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in islet cells of the pancreas, undifferentiated spermatogonia and other Neurog3 expressing cells. Transgene expression closely patterns endogenous gene expression as analyzed by in situ hybridiza ..... For more information please see the full phenotype on the strain data sheet | ||
| 006207 | STOCK Tg(Pcp2-cre)1Amc/J | Cryopreserved - Ready for recovery |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is observed in parasagittal domains of the cerebellum beginning at embryonic day 17 (E17). At E19, low level expression is observed in the most rostral lobe of cerebellum and expression broadens until all Purkinje cells express the transgene in adult mice. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome. These transgenic mice may be useful in studies utilizing "Cre-lox" technology, specifically regarding the nervous system, development and patterning of cerebellum, and cerebellar hypotrophy converse of Lhermitte-Duclos. Important Note: These mice also carry a GFP transgene that co-integrated with the Tg(Pcp2-cre)1Amc transgene. As such, GFP expression is reported in the cerebellum. | ||
| 012278 | STOCK Tg(Piwil1)1Ghan/J | Cryopreserved - Ready for recovery |
| This FLAG- and hemagglutinin (HA)-tagged Piwil1 (piwi-like homolog 1 (Drosophila); also known as Miwi) transgenic line enables researchers to selectively purify piwi-interacting RNA (piRNA) complexes using antibody-coated beads specific for the tagged elements. Expression, under the control of the transgene's native promoter, reproduces the developmentally timed pattern and subcellular localization of the endogenous protein. It begins at approximately postnatal day 14 (P14) in the pachytene stage of male germ cell meiosis and continues to adulthood. This strain may be helpful in studies of stem cell differentiation and spermatogenesis. | ||
| 012275 | STOCK Tg(Piwil2)1Ghan/J | Cryopreserved - Ready for recovery |
| This FLAG- and hemagglutinin (HA)-tagged Piwil2 (piwi-like homolog 2 (Drosophila); also known as Mili) transgenic line enables researchers to selectively purify piwi-interacting RNA (piRNA) complexes using antibody-coated beads specific for the tagged elements. Expression, under the control of the transgene's native promoter, reproduces the developmentally timed pattern and subcellular localization of the endogenous protein. It begins at approximately embryonic day 12.5 (E12.5) and protein is continuously present in germ cells until the haploid round spermatid stage in adults. This strain may be helpful in studies of stem cell differentiation and spermatogenesis. | ||
| 006129 | STOCK Tg(Zp3-EGFP)1Dean/J | Cryopreserved - Ready for recovery |
| Hemizygous mice are viable and fertile with no gross phenotypic abnormalities. These mice express the Zp3-EGFP fusion protein under the direction of the Zp3 promoter. As such, EGFP is readily detected throughout the width of the zona pellucida surrounding growing oocytes, with no expression in the surrounding somatic cells. Mutant mice have normal synthesis, intracellular trafficking, and secretion/incorporation into the zona pellucida matrix of the transgenic fusion protein. These mice may be useful in studies of reproductive biology, including intracellular trafficking of zona pellucida proteins to the cell surface, oocyte development, fertilization research, as well as developmental biology. | ||
| 008710 | B6.129P2(129S4)-Hprttm10(Ple162-EGFP/cre)Ems/Mmjax | Transferred |
| These Ple162-EGFPCre;mEMS954 mice have the Ple162-EGFPCre transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the human paired-like homeodomain 3 (PITX3) gene directing expression of an enhanced green fluorescent protein/Cre recombinase fusion protein (EGFPCre). These Ple162-EGFPCre;mEMS954 mice may be useful in studying PITX3-expressing cells in the brain and diseases affecting the brain, including mesocorticolimbic dopamine system function in the drug and natural reward circuitry of the brain, cognition, motivation, drug addiction, and psychiatric disorders.
For Ple162-EGFPCre;mEMS954 expression information, see The Pleiades Promoter Project website (Ple162 Promoter (pEMS1148)). The donating ..... | ||
| 009114 | B6.129P2(129S4)-Hprttm14(Ple103-EGFP/cre)Ems/Mmjax | Transferred |
| These Ple103-EGFP/cre;mEMS675 mice have the Ple103-EGFP/cre transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the human huntingtin-associated protein 1 (HAP1) gene directing expression of an enhanced green fluorescent protein/Cre recombinase fusion protein (EGFPCre). These Ple103-EGFP/cre;mEMS675 mice may be useful in studying HAP1-expressing cells in the brain and diseases affecting the brain.
For Ple103-EGFP/cre;mEMS675 expression information, see The Pleiades Promoter Project website (Ple103 Promoter (pEMS1083)). The donating investigator reports: | ||
| 009116 | B6.129P2(129S4)-Hprttm16(Ple167-EGFP/cre)Ems/Mmjax | Transferred |
| These Ple167-EGFP/cre;mEMS681 mice have the Ple167-EGFP/cre transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the human pogo transposable element with ZNF domain (POGZ) gene directing expression of an enhanced green fluorescent protein/Cre recombinase fusion protein (EGFPCre). These Ple167-EGFP/cre;mEMS681 mice may be useful in studying POGZ-expressing cells in the brain and diseases affecting the brain.
For Ple167-EGFP/cre;mEMS681 expression information, see The Pleiades Promoter Project website (Ple167 Promoter (pEMS1091)). The donating investigator reports: | ||
| 016603 | NOD.B6-Gt(ROSA)26Sortm1(HBEGF)Awai/DvsJ | Under Development for Cryo |
| Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration. For instance, the role of a particular cell population in T1D development can be determined by injecting Diphtheria toxin to deplete the specific cells in a timely fashion. For example, when bred to a strain expressing Cre recombinase in dendritic cells (see Stock No. 013233 for example), this mutant mouse strain may be useful for studies involving the immunology of diabetes. When crossed t ..... | ||
| 016223 | B6(Cg)-Tg(Phox2b-cre)3Jke/J | Under Development - Now Accepting Orders |
| Phox2b-Cre BAC transgenic mice are viable and fertile, with Cre recombinase expression under control of the Phox2b promoter/enhancer regions within the BAC transgene. cre-expressing neurons co-express PHOX2B (as shown by in situ hybridization). Phox2b-Cre BAC transgenic mice from founder line 3 exhibit cre expression directed primarily to the hindbrain; specifically the dorsal motor nucleus of the vagus (DMV) in parasympathetic visceral and branchial motor neurons, nodose sensory ganglia, and nucleus of the solitary tract (NTS) cells. No transgene expression is reported in hypothalamus or spinal cord. Although endogenous Phox2b expression is reported in peripheral ganglia along with the enteric nervous system, no peripheral transgene expression is reported for these Phox2b-Cre BAC transgenic mice. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequences in the offspr ..... For more information please see the full phenotype on the strain data sheet | ||
| 016224 | B6.129S(Cg)-Id2tm2.1Blh/ZhuJ | Under Development - Now Accepting Orders |
| The Id2-EGFP knockin allele was designed to both abolish inhibitor of DNA binding 2 (Id2) gene function and express enhanced green fluorescent protein (eGFP) from the Id2 promoter/enhancer elements. No mRNA or protein expression from the Id2-eGFP allele is observed. The donating investigator reports that homozygote mice mice are runted with defective lung alveolarization. Other organ systems have not been evaluated. However, Id2-eGFP homozygotes may be expected to exhibit the same phenotype as mice homozygous for other null mutations of this gene (including postnatal lethality and defects of the immune system, digestive tract, kidneys, adipose tissue and mammary gland development). The donating investigator also reports eGFP expression recapitulates the endogenous Id2 expression pattern. In the lungs, immunohistochemical detection of eGFP recapitulates the epithelial expression of the endogenous gene (distal tip lung epithelial multipotent precursor cells of the ..... For more information please see the full phenotype on the strain data sheet | ||
| 017593 | B6;129S-Sox2tm1(cre/ERT2)Hoch/J | Under Development - Now Accepting Orders |
| These Sox2-CreER knockin mice have the SRY-box containing gene 2 (Sox2) open reading frame replaced with a CreERT2 fusion gene. Sox2 is a widespread marker of pluripotent and many adult stem/progenitor cell types. Heterozygous mice are viable and fertile, while homozygous mice exhibit embryonic lethality. Following tamoxifen administration, Cre-ERT2 activity is observed in adult epithelial tissues; including testes, forestomach, glandular stomach, anus, cervix, esophagus, and lens, as well as glands associated with oral cavity, trachea, and cervix. When these mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the cre-expressing cells of the offspring. Cre-ERT2 activity is also detected in blastocysts, neural progenitor cells, and embryonic stem cell cultures following tamoxifen administration. These mice may be useful for stud ..... For more information please see the full phenotype on the strain data sheet | ||
| 017358 | B6;129S4-Tet1tm1.1Jae/J | Under Development - Now Accepting Orders |
| Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA carrying exon 4 or protein) is detected by RT-PCR or Western blot analysis of embryonic stem cells homozygous for the targeted mutation. Some residual full-length transcript lacking exon 4 is detected. No truncated protein is detected by Western blot. mESC from homozygotes have a reduced level of 5-hydroxymethylcytosine (5hmC). Although homozygotes are viable, at birth, 75% of homozygotes have a smaller body size than wildtype controls. Homozygous embryos at E12.5 have fewer tail somite pairs than wildtype controls. Homozygous crosses produce very few pups. | ||
| 016999 | B6;129S6-Gt(ROSA)26Sortm1(xstpx-rtTA2S*M2)Whsu/J | Under Development - Now Accepting Orders |
| These mice contain the rtTAS*M2, or rtTA2S-M2, an optimized form of reverse tetracycline regulated transactivator, gene inserted into the Gt(ROSA)26Sor locus. Expression of the rtTAS*M2 is blocked by a loxP-flanked STOP fragment placed between the rtTAS*M2 sequence and the Gt(ROSA)26Sor promoter. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will express rtTAS*M2, reverse tetracycline regulated transactivator, in the cre expressing cells. When bred to mice that also carry a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the gene of interest may be regulated by the tetracycline analog, doxycycline (dox). In the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not disp ..... For more information please see the full phenotype on the strain data sheet | ||
| 015855 | B6;DBA-Tg(Upk3a-GFP/cre/ERT2)26Amc/J | Under Development - Now Accepting Orders |
| These transgenic mice express the eGFPCreERT2 (Enhanced Green Fluorescent Protein and cre/ESR1) fusion gene under the direction of the mouse Upk3a, uroplakin 3A, promoter. Transgene expression is detected in the bladder urothelium and ureters of embryos, 15.5 embryonic days of age. Tamoxifen induced Cre recombinase activity is detected in the bladder of neonatal hemizygotes. GFP fluorescence is detected in cells lining the bladder lumen from embryos, 15.5 embryonic days of age. Mice that are hemizygous for the transgene are viable, fertile, normal in size and do not display physical or behavioral abnormalities.
This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 017353 | C.129S4(B6)-Il13tm1(YFP/cre)Lky/J | Under Development - Now Accepting Orders |
| The YetCre-13 allele has bicistronic IRES-YFP-Cre reporter/recombinase cassette inserted between the translational stop codon and the 3' UTR of the interleukin 13 (Il13) gene; thus expressing the endogenous gene and the YFP-Cre fusion protein (yellow fluorescent protein/humanized [mammalian codon-optimized] Cre recombinase). Homozygous YetCre-13 mice are viable, fertile, and normal in size. Expression of the YFP-Cre fusion gene is observed in Il13-expressing cells; including innate type 2 helper cells (Ih2 cells). These Ih2 cells are lineage-negative innate cells that arise during type 2 immunity or in response to the epithelial cytokines IL-25 and IL-33. Ih2 cells are widely distributed in mouse tissues and are particularly prevalent in mesenteric lymph nodes, spleen, and liver. These YetCre-13 mice may be useful for fluorescent reporter and/or Cre-lox studies of IL-13-expressing cells in type 2 immunity/host response to allergens and parasitic helminths. They may als ..... For more information please see the full phenotype on the strain data sheet | ||
| 016617 | C57BL/6-Tg(Nr4a1-EGFP/cre)820Khog/J | Under Development - Now Accepting Orders |
| Mice hemizygous for the Nur77-GFPCre BAC transgene (Nur77GFP BAC transgenic mice) are viable and fertile with normal lymphoid and myeloid development. Nur77-GFPCre BAC transgenic mice express an enhanced green fluorescent protein/codon-optimized "humanized" Cre recombinase fusion protein (eGFP-hCre) under control of the Nr4a1 (Nur77) promoter/enhancer regions within the BAC transgene. Nur77-GFPCre BAC transgenic mice from founder line B6-820 exhibit GFP expression patterns consistent with endogenous Nur77. Specifically, GFP is highly expressed in a subset of myeloid lineage cells of the spleen (but not lymph node), while low levels of GFP are observed in T and B lymphocytes. GFP is up-regulated by antigen receptor stimulation in Nur77-GFPCre BAC transgenic mice, but unlike the CD69 marker of T cell activation, GFP is not induced by inflammatory stimuli. Furthermore, the level of GFP expressed during acute activation reflects the strength of T cell receptor (TCR) stim ..... For more information please see the full phenotype on the strain data sheet | ||
| 014180 | STOCK Myocdtm1(cre)Jomm/J | Under Development - Now Accepting Orders |
| This strain expresses Cre recombinase from the endogenous Myocd locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs during early development, at embryonic day 7.5, in the developing heart, dorsal aorta, head mesenchyme and in the somites beginning at embryonic day 8.5. Cre activity is also detected in skeletal muscle fibers and vasculature. Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. | ||
| 011103 | STOCK Olig2tm2(TVA,cre)Rth/J | Under Development - Now Accepting Orders |
| In this targeted mutation strain, the mouse Olig2 (oligodendrocyte transcription factor 2) locus drives expression of cre and tva (an avian-specific retroviral receptor) in Olig2-expressing cells of the central nervous system (CNS). When bred with mice containing a sequence flanked by similarly oriented loxP sites, flanked sequences will be deleted in the Cre-expressing tissues of the offspring. Injection of avian retrovirus (e.g., RCAS) can specifically infect Olig2-tva-expressing cells permitting cell labeling with reporters or forced expression of genes. This strain may be useful in studies of neurodevelopment. Homozygotes are not viable due to the diruption of Olig2 function. | ||
| 012452 | STOCK Tg(Rr5-GFP/cre)1Sapc/J | Under Development - Now Accepting Orders |
| Hoxb4-ENE-GFPCre transgenic mice are viable and fertile, with expression of a GFP/Cre fusion protein directed primarily to developing spinal cord and hindbrain by the mouse homeobox B4 early neuronal enhancer (ENE) region and heat shock protein 1B minimal promoter. Cre recombinase activity is observed from embryonic day (E)9.0-13.5 throughout the neural tube in the hindbrain, the dorsal root ganglia, motor neurons, and the Xth cranial ganglia (vagus); with a sharp anterior boundary at rhombomeres 6 and 7 (r6/r7). Ectopic cre activity is reported in the Vth cranial nerve (trigeminal) of E10.5-11.5 embryos. GFP fluorescence is first detectable at E9.0, strongest at E10.5, and absent by ~E12.0. GFP fluorescence is localized to the neural tube and the hindbrain with a sharp anterior limit between rhombomeres 7 and 8 (r7/r8) at E9.5. GFP expression at the anterior r7/r8 boundary was maintained through E11.5, while the posterior boundary expanded caudally from E9.5-E11.5. When bred wi ..... For more information please see the full phenotype on the strain data sheet | ||
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