Search Criteria: Research Area is "Research Tools: Genetics Research (Tissue/Cell Markers: fos/luc)"
| Stock Number |
Strain Name Strain Description |
Standard Supply |
| 006102 | B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J | Repository- Live |
| Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator reports that homozygous females in their colony are subfertile. These transgenic mice express luciferase under the regulation of the Il2 minimal promoter and 3 binding sites for the NFAT inducible nuclear factor involved in the regulation of interleukin-2 and other cytokine expression. Luciferase activity in these transgenic mice identifies NFAT-mediated transcription. These NFAT-luc transgenic mice may be useful be useful as reporters for NFAT-mediated expression during thymocyte development and selection and in studies related to signal transduction, apoptosis, and transcriptional regulation. | ||
| 006100 | B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Mutant mice have the luciferase gene driven by two copies of the NF-kappaB (NF-kB or NFkB) regulatory element. The presence of nuclear NF-kB DNA binding activity (as detected by electrophoretic mobility shift assay [EMSA]) is consistent with luciferase reporter activity; these reporter mice identify NF-kB transcriptional activity in any tissue. These transgenic mice may be useful in studies of immunology, cellular signaling, signal transduction, apoptosis, and transcription factor function. | ||
| 006098 | B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Homozygous females are subfertile. These transgenic mice express luciferase under the regulation of the Il2 minimal promoter and 3 binding sites for NFAT, an inducible nuclear factor involved in the regulation of interleukin-2 and other cytokine expression. Luciferase activity in these transgenic mice identifies NFAT-mediated transcription. These mice may be useful as reporters for NFAT-mediated expression during thymocyte development and selection and in studies related to signal transduction, apoptosis, and transcriptional regulation. | ||
| 003479 | B6.C3-Tg(Fos-luc)1Rnd/J | Repository-Cryopreserved |
| C57BL/6-TgN(c-fosLuc)1Rnd mice are viable and fertile. They carry a firefly luciferase reporter gene driven by the c-fos promoter. The luciferase transgene is expressed constitutively in all tissues of these mice, at the lowest levels in kidney, liver, lung, spleen, heart and various areas of the brain, and at the highest levels in skin and testis. Activation of the c-fos promoter in response to a wide range of stimuli results in increased luciferase expression. C57BL/6-TgN(c-fosLuc)1Rnd mice provide a unique system to monitor c-fos promoter activity in living tissue explant or dispersed cell cultures. | ||
| 006101 | B6.Cg-Tg(TRE/Prl-luc)31FlvRinc/J | Repository-Cryopreserved |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Naïve (unstimulated) cells from transgenic mice show no luciferase activity. When activated via addition of phorbol myristate acetate (PMA) plus ionomycin, cell populations from hemizygous mice exhibit high luciferase transcriptional activity in CD4+ T cells. Identically treated single-positive CD8+ T cells have lower transcriptional activity, while no luciferase is observed in B cell populations. Interleukin-2 transcriptional activators (anti-CD3 and ConA) also promote similar cell-specific patterns of reporter transcription. Luciferase expression is cyclosporine sensitive. Nuclear proteins isolated from hemizygous splenocytes show AP-1 specific binding to the transgenic TRE domain. This reporter transgene faithfully identifies AP-1 transcriptional activity both in vivo and in vitro. These mice may be useful in studies of immu
..... For more information please see the full descriiption on the strain data sheet | ||
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