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New Strains Under Development

JAX® Mice Strains

Stock
Number
Strain Name
 
Phenotype
Standard Supply
002468 KK.Cg-Ay/J
Level 2
Ay and other mutations at the a locus conferring a completely yellow coat color are dominant to all a alleles that produce a darker coat. Hair pigment of Ay heterozygotes is yellow, but eyes are black. Heterozygotes usually become obese and infertile within a few months after birth. Increased adipose tissue mass is due to fat cell hypertrophy, and it has been hypothesized that the obesity results from the observed reduction in hypothalamic norepinephrine and dopamine. Heterozygotes are more susceptible to several kinds of tumors than are normal mice, possibly due, at least in part, to a general increase in cell proliferation that also manifests as a slight increase in lean body mass and skeletal length. Further spleen cells from heterozygotes cause a significantly lower graft vs. host reaction. Mice homozygous for the yellow spontaneous mutation (Ay) die before implantation, or shortly thereafter. The time of de .....
For more information please see the full phenotype on the strain data sheet
003291 C57BL/6-Tg(CAG-EGFP)1Osb/J
Level 4
This transgenic mouse line with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer makes all of the tissues, with the exception of erythrocytes and hair, appear green under excitation light. Note that mice homozygous for this transgene die within the first two weeks following birth.
005867 B6.129-Indotm1Alm/J
Repository- Live
Homozygous mice are viable and fertile with normal immune system development and function. They exhibit no spontaneous autoimmune disorders. No gene product (mRNA or protein) from the targeted gene is detected in the epididymis. At embryonic day 10.5, endogenous protein is absent from all cells at the maternal-fetal interface when both parents are homozygous for the targeted gene. Allogeneic and syngeneic pregnancy outcomes are unaffected by this mutation. In contrast to wildtype, anti-proliferative treatments (CTLA4-Ig, IFNalpha, or CpG-ODN) do not suppress T cell expansion both in vivo and in vitro. In addition, homozygous dendritic cells isolated from lymph nodes draining (induced) tumor sites have no suppressor activity. These mice may be useful in studies of pregnancy and reproductive immunology (tryptophan degradation, T cell activation, clonal expansion) as well as autoimmune disease, tissue transplantation, fostering, acquired tolerance/T cell anergy, and immunosu .....
For more information please see the full phenotype on the strain data sheet
006262 B6.129P2-Fut2tm1Sdo/J
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Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice have a chinchilla (gray) coat color, while heterozygotes have the typical black coat color expected for the C57BL/6J genetic background. Alpha (1,2) fucosylated glycans are not detected in the uterine epithelia from homozygotes at estrus. Beta galactosidase staining is detected in endocervial and uterine gland mucus secreting cells, stomach foveolar pit cells and chief cells, and colon goblet cells. The pattern of beta galactosidase activity mimics the endogenous expression pattern of the endogenous gene. This mutant mouse strain represents a model of the nonsecretor ABH histo-blood group antigen, which confers resistance to Norwalk virus infection, and may be useful in studies of reproductive biology, gastrointestinal tract epithelium, and the function of fucosylated glycans.

This strain was transferred fr .....
For more information please see the full phenotype on the strain data sheet
006233 B6.129S1-Casp3tm1Flv/J
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On this C57BL/6 congenic background, homozygotes are viable, fertile, and reach adulthood, but females reported display suboptimal mothering instincts. Functional endogenous protein and mRNA are absent from all tissues tested. Homozygous mice are resistant to in vivo cerebral ischemia/reperfusion and in vitro oxygen-glucose deprivation. Ovaries from female homozygotes show aberrant atretic follicles associated with a granulosa cell-intrinsic defect in apoptosis as well as defective corpus luteum regression. Homozygous mice are congenitally deaf with hair cell defects in the Organ of Corti. Optic lens formation/morphology also is abnormal with cataracts at the anterior lens pole. Of note, these mice lack the embryonic/perinatal-lethal brain pathology observed in mutant mice on the 129 and mixed B6;129 genetic backgrounds. These mutant mice may be useful in studies of apoptosis, ovarian follicle and corpus luteum development, and eye and ear development.
007668 B6.129S4(Cg)-Arntltm1Weit/J
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Mice homozygous for this conditional Bmal1 (floxed) allele possess loxP sites flanking exon 8 of the targeted gene and are viable and fertile, with circadian behavioral rhythms indistinguishable from wildtype littermates. When bred to mice that express Cre recombinase, the resulting offspring will have the exon encoding the BMAL1 basic helix-loop-helix (bHLH) domain deleted in the cre-expressing tissue(s). These Bmal1-floxed mutant mice may be useful in generating conditional mutations (whole-mouse or tissue-specific) to study the role of circadian clock/circadian rhythm in physiological and behavioral regulation.

For example, when crossed to a strain expressing Cre recombinase in the retina (see Stock No. 005105), this mutant mouse strain may be useful in studies of the circadian clock of the retina.

005897 B6.129S4-Ppardtm1Rev/J
Repository- Live
These mice possess loxP sites on either side of exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 4 of the targeted gene is deleted in the tissue of interest, leading to premature termination of the translation product upstream of the DNA binding domain. This strain may be useful in generating tissue-specific mutants of the floxed allele for use in studies including embryo development, adipocyte physiology, fat metabolism and storage, inflammation, and cancer.
002192 B6.129S7-Gt(ROSA)26Sor/J
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Mice heterozygous or homozygous for the ROSA26 retroviral insertion display no distinguishing phenotype. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult mouse.
000021 B6.Cg-Ay/J
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Mice homozygous for the yellow spontaneous mutation (Ay) die before implantation or shortly thereafter. The time of death and type of abnormality is, in part, determined by the genetic background on which the mutation is placed. Hair pigment in heterozygous mice is yellow, but eyes are black. Heterozygotes usually become obese and infertile after the first few months. Increased adipose tissue mass is due to fat-cell hypertrophy. It has been hypothesized that the obesity results from the observed reduction in hypothalamic norepinephrine and dopamine levels. Insulin resistance and hyperglycemia follow development of hyperinsulinemia in early adulthood, although the degree is less severe than on the KK/UpJ genetic background (Stock No. 002468). Heterozygotes are also more susceptible to several kinds of tumors than normal mice, and their spleen cells cause a significantly lower graft vs. host reaction. The level of .....
For more information please see the full phenotype on the strain data sheet
007484 B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
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On an albino background Tg(Tyr)3412ARpw permits the identification of gender as early as embryonic day 10.5. This strain is segregating for Tg(Tyr)3412ARpw and homozygous for Tyrc-2J so the individuals not carrying Tg(Tyr3412)ARpw are albino. Because Tg(Tyr)3412ARpw inserted into the X Chromosome, breeding a carrier male with a noncarrier (wild-type) female results in embryos in which all XX individuals develop eye pigment, due to the Tg(Tyr)3412ARpw inherited from their father, while all XY individuals have non-pigmented eyes, having inherited a wild-type X Chromosome from their mother.

This strain is also homozygous for Tg(SryEGFP)92Ei. This reporter transgene consists of a 5 prime regulatory segment of the Sry gene driving EGFP. This transgene is expressed in the pre-support cell lineage (pre-sertoli and pre-granulosa cells) of the fetal genital ridge (Albrecht and Eicher, 2001) and in discrete areas the adult male but not female brain (Dewing et al., 20 .....
For more information please see the full phenotype on the strain data sheet

006881 B6.Cg-Tg(Aqp2-cre)1Dek/J
Repository- Live
Mice hemizygous for this AQP2-Cre transgene are viable and fertile. Transgenic cre activity, directed by the mouse aquaporin 2 promoter, is observed in kidney cells (collecting duct) and testes (sperm). When bred with mice containing a loxP-flanked sequence of interest, cre-mediated recombination will result in deletion of the flanked sequence. In such breedings, maternal inheritance of the transgene is recommended for kidney-specific recombinase activity as males express cre in sperm as well as kidney tissues. These AQP2-Cre mice may be used to generate conditional mutations in the renal collecting duct for studying nephrology, physiology, metabolism, or type II diabetes. In addition, cre expression in sperm may be useful in generating conditional mutations in multiple or all tissues in the resulting offspring.
002709 B6;C3-Tg(TettTALuc)1Dgs/J
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Transgenic mice were produced by co-microinjection of modified tetracycline-controlled transactivator (tTA) and luciferase genes placed under the control of tetracycline-responsive promoter elements (TREs). Expression of tTA in transgenic mice is both inducible and autoregulatory; expression of the transgenes are suppressed in the presence of tetracycline and highly expressed in its absence. Induced transgene expression levels are higher than those achieved in strains utilizing a constitutive tTA system. Luciferase expression (in the absence of tetracycline) has been demonstrated in all organs examined - spleen, thymus, lung, liver, kidney, heart, cerebrum, cerebellum, lymph nodes, testes. High activity is found in thymus and lung; low expression is found in liver and kidney. Induction ranges from 2-fold in testes to 150-fold in thymus. The founder line of this strain is #17, as reported in the primary reference. Homozygous mice are viable and fertile.
004654 B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J
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Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein under the control of the POU domain, class 5, transcription factor 1, promoter and distal enhancer. Primordial germ cell specific markers, alkaline phosphatase II and stage-specific embryonic antigen, are co-expressed in EGFP positive cells. 9.5 and 10.5 dpc (days post-coitum) migratory primordial germ cells from hemizygotes and homozygotes can be sorted and isolated by flow cytometry. This strain represents an effective tool for studying genetic imprinting and early embryonic development.
003651 C57BL/6-Tg(Zp3-cre)93Knw/J
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This is a transgenic line in which cre expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene. This promoter normally directs expression exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain would be useful for deleting a floxed sequence specifically in the female germ line. The Donating Investigator suggests to accomplish this, females homozygous or heterozygous for the floxed allele, as well as hemizygous for the Zp3cre allele are crossed with wild type males. Progeny will carry the deleted-floxed allele.
003927 C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ
Repository- Live
Tg(Sry-EGFP)92Ei has been shown to drive expression of GFP in pre-Sertoli and pre-granulosa cells of the genital ridge, with expression stronger in homozygotes than hemizygotes. Gonadal expression is not found in germ cells or vascular endothelial cells, but is restricted to a subset of somatic cells. Expression in the genital ridge begins in the center and moves out to the poles, with a bias toward the caudal pole. GFP expression is also found in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males, but not females, with lower levels of expression observed sparsely throughout the cortex. Strongest expression in the male brain is in the pars compacta and, at the cellular level, both cytoplasmic and nuclear localization are found.
006177 CD3/JlsJ
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Swiss derived CD-1 mice are less responsive to exogenous estradiol than most inbred strains as measured by resistance to decreases in testes weight, spermatogenesis, and spermatid development. Derived from CD-1, the inbred strains CD3/JlsJ, CD7/JlsJ, and CD9/JlsJ mice exhibit a moderate sensitivity to endocrine disruption by estradiol, while CD10/JlsJ mice are highly resistant to endocrine disruption by estradiol. All lines generated in the selection program are significantly less responsive to estradiol than C57BL/6 mice.
The selectively bred strains CD3/JlsJ (006177), CD7/JlsJ (006178), CD9/JlsJ (006179) and CD10/Jls (006180) may serve as tools for mapping and characterizing genes responsible for estradiol sensitivity.

SNP data is available for this stra .....
For more information please see the full phenotype on the strain data sheet

006178 CD7/JlsJ
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Swiss derived CD-1 mice are less responsive to exogenous estradiol than most inbred strains as measured by resistance to decreases in testes weight, spermatogenesis, and spermatid development. Derived from CD-1, the inbred strains CD3/JlsJ, CD7/JlsJ, and CD9/JlsJ mice exhibit a moderate sensitivity to endocrine disruption by estradiol, while CD10/JlsJ mice are highly resistant to endocrine disruption by estradiol.(personal communication) All lines generated in the selection program are significantly less responsive to estradiol than C57BL/6 mice.
The selectively bred strains CD3/JlsJ (006177), CD7/JlsJ (006178), CD9/JlsJ (006179) and CD10/Jls (006180) may serve as tools for mapping and characterizing genes responsible for estradiol sensitivity.
SNP data is available for this strain on the Wellcome Trust Centre for Human Genetics site.
006179 CD9/JlsJ
Repository- Live
Swiss derived CD-1 mice are less responsive to exogenous estradiol than most inbred strains as measured by resistance to decreases in testes weight, spermatogenesis, and spermatid development. Derived from CD-1, the inbred strains CD3/JlsJ, CD7/JlsJ, and CD9/JlsJ mice exhibit a moderate sensitivity to endocrine disruption by estradiol, while CD10/JlsJ mice are highly resistant to endocrine disruption by estradiol.(personal communication) All lines generated in the selection program are significantly less responsive to estradiol than C57BL/6 mice.
The selectively bred strains CD3/JlsJ (006177), CD7/JlsJ (006178), CD9/JlsJ (006179) and CD10/Jls (006180) may serve as tools for mapping and characterizing genes responsible for estradiol sensitivity.
SNP data is available for this strain on the Wellcome Trust Centre for Human Genetics site.
006954 FVB-Tg(Ddx4-cre)1Dcas/J
Repository- Live
Mice hemizygous for this Vasa-Cre transgene are viable and fertile. Transgenic cre activity is directed to male and female germ cells starting at embryonic day (e)15-e18. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequence. In such breedings, occasional hemizygous mice may exhibit variegated cre expression in skin epithelium or global cre expression (<20% incidence). Differential parent-of-origin transgene expression is observed. When the mother harbors Vasa-Cre, virtually all progeny undergo global Cre-mediated recombination, even those that do not inherit the transgene (which may be useful in converting a "floxed" allele to a null while obviating the need to perform additional crosses to remove the transgene). To achieve germ-line specific Cre-mediated recombination in offspring, paternal Vasa-Cre mice should be used. These Vasa-Cre mice may be useful in generating c > .....
For more information please see the full phenotype on the strain data sheet
006875 FVB/N-Tg(Tagln-rtTA)E1Jwst/J
Repository- Live
Transgenic SM22-rtTA mice are viable and fertile. These mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the murine SM22-alpha (SM22a or transgelin) promoter. When hemizygotes are mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in the bitransgenic offspring is inducible in smooth muscle cells with administration of the tetracycline analog, doxycycline. These SM22-rtTA mice provide a "Tet-On" tool that allows the inducible expression of genes in smooth muscle cells.
003116 STOCK Tg(CAG-EGFP)D4Nagy/J
Repository- Live
This transgenic strain carries the Enhanced Green Fluorescent Protein (Clontech) driven by chicken beta-actin promoter and CMV intermediate early enhancer. The transgene was found to have integrated on the X chromosome. Mice of this strain, and cells derived from them, can be distinguished from wildtype on the basis of fluorescence. Transgene expression is seen as early as ~embryonic day 2.75, as morula stage embryos begin to compact. Expression of EGFP is spatiotemporally widespread, though levels differ between different lineages (e.g., expression is slightly higher in the heart). As the transgene is located on the X chromosome, transgenic males will exclusively transmit the EGFP transgene to their female offspring, providing the basis for a non-invasive sexing assay based on green fluorescence. These mice may be useful for sex discrimination prior to overt sexual dimorphism (embryonic day 12.5), monitoring X-inactivation, and may be a source of fluorescently marked cells/tiss .....
For more information please see the full phenotype on the strain data sheet
007896 STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
Repository- Live
Mice hemizygous for this ROSA26-EGFP BAC transgene are viable and fertile, with widespread EGFP expression in all organs and tissues analyzed. In addition, stable and uniform EGFP expression is observed in differentiated progeny of transplanted hematopoietic stem cells (HSC) isolated from ROSA26-EGFP mice. These ROSA26-EGFP BAC transgenic mice may be useful for fluorescent labeling of cells and tissues both in vivo and in vitro, are suitable for use in histological preparations, and are appropriate as a source of fluorescently labeled donor cell populations (such as tracking hematopoietic cell fate in transplant recipients).
006129 STOCK Tg(Zp3-EGFP)1Dean/J
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Hemizygous mice are viable and fertile with no gross phenotypic abnormalities. These mice express the Zp3-EGFP fusion protein under the direction of the Zp3 promoter. As such, EGFP is readily detected throughout the width of the zona pellucida surrounding growing oocytes, with no expression in the surrounding somatic cells. Mutant mice have normal synthesis, intracellular trafficking, and secretion/incorporation into the zona pellucida matrix of the transgenic fusion protein. These mice may be useful in studies of reproductive biology, including intracellular trafficking of zona pellucida proteins to the cell surface, oocyte development, fertilization research, as well as developmental biology.
002484 129-Alpltm1Sor/J
Repository-Cryopreserved
Mice heterozygous for the Alpltm1Sor targeted mutation appear normal and viable. Homozygous mutant mice are perinatal lethals but can be rescued by pyridoxal treatment. Surviving homozygotes develop epilepsy due to reduced GABA levels in the brain. Bone formation does not appear to be grossly affected in untreated animals, but treated animals exhibit cranial dysmorphology. The targeting vector contained both a b-galalactosidase and a neomycin genes (beta-geo), both of which are under the control of the Alpl promoter and are thus expressed in a tissue specific manner. Specifically, expression occurs in developing bones and in primordial germ cells (PGC), and the beta-galactosidase thus serves as a marker for these tissues. The marker for PGC's is particularly significant because the current marker (alkaline phosphatase staining) is only useful to study early germ cell migration.
005901 B6.129S4-Ppardtm2Rev/J
Repository-Cryopreserved
Heterozygous mice are viable and fertile. All homozygous mice die in utero. Macrophages homozygous for this mutation have no transcriptional response to very low-density lipoprotein treatment. No evaluation of lacZ expression is published. The donating investigator reports homozygous mice on this background have a similar, albeit earlier, embryonic phenotype as the exon 4 deleted mutants described in other publications (Barak PNAS 2002 99:303-8, Chawla PNAS 2003 100:1268-73, and Lee Science 2003 302:453-7). Heterozygous mice or homozygous embryo-derived cells may be useful in studies of embryo development, adipocyte physiology, fat metabolism and storage, inflammation, and cancer.
003823 B6.129S4-Ttpatm1Far/J
Repository-Cryopreserved
Mice that are homozygous null for the Ttpa gene are viable, normal in size and do not display any gross physical abnormalities. The Ttpa protein product is required for the maintenance of proper alpha-tocopherol levels, the major form of vitamin E in plasma and tissues. The absence of Ttpa protein product in homozygous-null animals results in a corresponding 95% reduction in alpha-tocopherol. Low levels of alpha-tocopherol render female mice infertile, a condition that can be addressed with vitamin E supplements. Male fertility is unimpaired. These mice provide a viable model for studying vitamin E deficiency.
004267 B6.129S6-Dnmt3ltm1Bes/J
Repository-Cryopreserved
Mice that are homozygous for this targeted allele are viable, normal in size, do not display any gross physical or behavioral abnormalities, but are sterile. Adult homozygous males display severe hypogonadism, Sertoli-cell-only phenotype and apoptotic death of spermatocytes prior to pachytene with loss of spermatogonia progressing to nonsyndromic azoospermia in later adulthood. Extreme abnormalities of meitotic pairing occur. There is a failure to methylate transposons in prospermatogonia with expression of very high levels of both LTR and non-LTR transposons in spermatogonia and spermatocytes. Homozygous females have normal oogenesis. Heterozygous progeny of homozygous females do not develop past 9.5 day post coitum, with pericardial edema with exencephaly and other neural tube defects. Maternally imprinted gene sequence that is usually heavily methylated in control oocytes is undermethylated in mutant mice oocytes. Paternally imprinted gene sequence is not effected and global genome .....
For more information please see the full phenotype on the strain data sheet
002741 B6.129S7-Alpltm1Sor/J
Repository-Cryopreserved
Mice heterozygous for the Alpltm1Sor targeted mutation appear normal and viable. Homozygous mutant mice are perinatal lethals but can be rescued by pyridoxal treatment. Surviving homozygotes develop epilepsy due to reduced GABA levels in the brain. Bone formation does not appear to be grossly affected in untreated animals, but treated animals exhibit cranial dysmorphology. The targeting vector contained both a b-galalactosidase and a neomycin genes (beta-geo), both of which are under the control of the Alpl promoter and are thus expressed in a tissue specific manner. Specifically, expression occurs in developing bones and in primordial germ cells (PGC), and the beta-galactosidase thus serves as a marker for these tissues. The marker for PGC's is particularly significant because the current marker (alkaline phosphatase staining) is only useful to study early germ cell migration.
006407 B6.Cg-Gusbmps/BrkJ
Repository-Cryopreserved
Mice homozygous for the "mps" (mucopolysaccharidosis type VII or MPS VII) mutation are devoid of expression of the lysosomal enzyme beta glucuronidase. Homozygous animals are viable, but females have a deficiency in lactation. Skeletal and connective tissue anomalies in both males and females are believed to prevent successful breeding. As this mutation is recessive, heterozygous mice are phenotypically similar to wildtype. Homozygotes exhibit short and thickened long bones (smaller than heterozygous or wildtype littermates), "pug type" appearance of the nose, hepatomegaly, splenomegaly, corneal clouding, and deafness. These mice have the H2b haplotype typical of inbred C57BL/6 mice. MPS VII mice are a model of the beta glucuronidase enzyme deficiency in humans called Sly Disease. They may be useful in developing new therapies (enzyme replacement, cell transplantation, gene therapy) broadly applicable to other lysosomal storage diseases.
002283 B6.Cg-KitW-19H/EiJ
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This deletion is a dominant mutation causing white spotting on the feet, tail, belly, and occasionally elsewhere. Homozygosity is embryonic lethal. On the C57BL/6 background approximately 60% of heterozygous females have a closed vagina. The ovaries from these heterozygotes are normal and fine for transplantation. On the C57BL/6 background in the presence of the YAKR/J Chromosome (available from Stock No. 007250), KitW-19H heterozygosity results in sex reversed XY females.
004153 B6;129S-Mtap7Gt(ROSABetageo)1Sor/J
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At birth, mice homozygous for the gene-trapped Mtap7 allele are viable, normal in size and do not display any gross physical or behavioral abnormalities. Although trace amounts of a presumably nonfunctional transcript can be detected in testis tissue, no protein product is immunodetectable. Male homozygotes are sterile. Expression of the reporter gene (B-galactosidase from the Bgeo fusion gene) employed by the gene trap vector indicates that the Mtap7 promoter directs expression in various tissues with highest levels seen in the seminiferous tubules. During the first wave of spermatogenesis at 5 weeks of age, deformed spermatids can be observed. Abnormalities are attributed to aberrant microtubule organization. Microtubule aberrations are also observed in Sertoli cells. Gradual loss of germ cells occurs. At three months of age, the testes of homozygous mutants are less than one-third the size of those of heterozygous littermates.
002317 B6;129S7-Alpltm1Sor/J
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Mice heterozygous for the Alpltm1Sor targeted mutation appear normal and viable. Homozygous mutant mice are perinatal lethals but can be rescued by pyridoxal treatment. Surviving homozygotes develop epilepsy due to reduced GABA levels in the brain. Bone formation does not appear to be grossly affected in untreated animals, but treated animals exhibit cranial dysmorphology. The targeting vector contained both a b-galalactosidase and a neomycin genes (beta-geo), both of which are under the control of the Alpl promoter and are thus expressed in a tissue specific manner. Specifically, expression occurs in developing bones and in primordial germ cells (PGC), and the beta-galactosidase thus serves as a marker for these tissues. The marker for PGC's is particularly significant because the current marker (alkaline phosphatase staining) is only useful to study early germ cell migration.
004966 B6;CBA-Tg(Acrv1-EGFP)2727Redd/J
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Mice carrying this "-408SP10-gfp" transgene express Enhanced Green Fluorescent Protein (EGFP) under the direction of the -408 to +28 bp mouse SP-10 (Acrv1) promoter region that contains the necessary transcription regulation signals responsible for both temporal and testis-specific gene expression in transgenic mice. In hemizygous mice, EGFP fluorescence is detected in postmeiotic round spermatid cells of testis beginning from post natal day 21 through adult life. Transgene expression is detected in testis by by northern hybridization as well as RT-PCR analysis. In situ hybridization examination of testis from transgenic mice reveals EGFP is expressed in a pattern mimicking endogenous Acrv1 spatial and temporal expression. This strain may be useful in studies related to spermatogenesis.
003466 B6;D2-Tg(Sycp1-cre)4Min/J
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002369 B6;SJL-Tg(c177-lacZ)226Bri/J
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Transgenic mice show a strong expression of the lacZ gene in round spermatids and subsequent stages of spermatogenesis. These mice can be used as a source of germ cells and transplanted into busulfan treated mice (busulfan (40 mg/kg i.p.) destroys spermatogenic stem cells). LacZ serves as a reporter for the transplanted cells. Following transplantation, spermatogonia with stem cell potential establish themselves on the basal membrane of the recipient seminiferous tubule and begin to replicate. This strain has a higher copy number of the transgene than the TgN(c177lacZ)227Bri strain and may be distinguished from it by a dot blot. Cells from these two lines may therefore be mixed prior to injection and be distinguished from each other in the progeny.
002372 B6;SJL-Tg(c177-lacZ)227Bri/J
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B6SJLF1/J mice (Stock No. 100012) may be used as controls. These only provide an approximate genetic match to this B6,SJL background.
003299 B6;SWJ-Tg(TIMP3-lacZ)7Jeb/J
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These transgenic mice show no phenotypic defects. LacZ expression is observed in the eye, specifically in the corneal endothelium and ganglion cell layer. LacZ expression is also observed in the kidney, choroid plexus, testes and ovaries, gingiva, bone and the hair follicles of the skin. Transgene expression is developmentally regulated.
006430 B6Ei.129(Cg)-Figlatm1Dean/EiJ
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Heterozygous female and homozygous male mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Female homozygous mice are infertile due to absence of primordial follicles in the ovary. No gene product (mRNA) is detected by RNase protection analysis of total ovarian RNA. This mutant mouse strain may be useful in studies of female infertility.
007041 B6Ei.129P2-Nr5a1tm2Klp/EiJ
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Mice homozygous for this floxed allele are viable and fertile. These mutant mice have loxP sites flanking the C-terminal coding exon. When bred to Cre-recombinase expressing mice, offspring will have a deletion of this exon in the cre expressing tissue(s). These floxed mice may be useful in studying steroidogenic factors and pituitary gonadotrope function.

For example, when crossed to a strain expressing Cre recombinase in the anterior and intermediate lobes of the pituitary gland (see Stock No. 004426), this mutant mouse strain may be useful in studies of pituitary gonadotrope function.

002021 B6Ei.LT-Y*/EiJ
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007265 C57BL/6-Tg(Sry-EGFP)92Ei Chr YAKR/J/EiJ
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007264 C57BL/6-Tg(Sry-EGFP)92Ei Tg(Sry)4Ei Chr YPOS/EiJ
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003394 C57BL/6-Tg(Zp3-cre)3Mrt/J
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This is a transgenic line in which cre expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene. This promoter normally directs expression exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain would be useful for deleting a floxed sequence specifically in the female germ line. Homozygotes females produce small litters (3.7 pups/litter; Genesis 26:110-112, 2000). An alternative transgenic strain that bears a similar transgenic construct and produces larger litters (~6 pups/litter) is available: C57BL/6-Tg(Zp3-cre)93Knw/J (Stock No. 003651).
007250 C57BL/6JEi-Chr YAKR/J/Ei
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This consomic is valuable in the study of sex determination. The TOrl /+ XYAKR/J offspring, from the cross of B6.Cg-TOrl /+ females with C57BL/6JEi-Chr YAKR/J/EiJ males, are sex reversed, with ovarian tissue development.
002890 C57BL/6JEi-Chr YAPP/EiJ
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002605 C57BL/6JEi-Chr YMA/MyJ/EiJ
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This consomic is valuable in the study of sex determination. The TOrl /+ XYMA/MyJ offspring, from the cross of B6.Cg-TOrl /+ females with C57BL/6JEi-Chr YMA/MyJ/EiJ males, are sex reversed, with ovarian tissue development.
002604 C57BL/6JEi-Chr YRF/J/EiJ
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This consomic is valuable in the study of sex determination. The TOrl /+ XYRF offspring, from the cross of B6.Cg-TOrl /+ females with C57BL/6JEi-Chr YRF/J males, are sex reversed, having ovarian tissue development.
001574 C57BL/6JEi-Chr YWSB/Ei/EiJ
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This consomic is valuable in the study of sex determination. The TOrl /+ XYWSB offspring, from the cross of B6.Cg-TOrl /+ females with C57BL/6JEi-Chr YWSB/EiJ males, are sex reversed, with ovarian tissue development.
002063 C57BL/6JEi-Tg(Sry)1Ei Chr YAKR/J/EiJ
Repository-Cryopreserved
In this strain, XX mice that carry the Tg(Sry)1Ei transgene are sex-reversed males. XYAKR/J mice heterozygous for TOrl on a C57BL/6J background develop as sex reversed females, but in the presence of the Tg(Sry)1Ei transgene these XY mice develop as normal males with testes.
002708 C57BL/6JEi-Tg(Sry)2Ei Chr YAKR/J/EiJ
Repository-Cryopreserved
In this strain, XX mice that carry the Tg(Sry)2Ei transgene are sex-reversed males.
006180 CD10/JlsJ
Repository-Cryopreserved
Swiss derived CD-1 mice are less responsive to exogenous estradiol than most inbred strains as measured by resistance to decreases in testes weight, spermatogenesis, and spermatid development. Derived from CD-1, the inbred strains CD3/JlsJ, CD7/JlsJ, and CD9/JlsJ mice exhibit a moderate sensitivity to endocrine disruption by estradiol, while CD10/JlsJ mice are highly resistant to endocrine disruption by estradiol. All lines generated in the selection program are significantly less responsive to estradiol than C57BL/6 mice.
The selectively bred strains CD3/JlsJ (Stock No. 006177), CD7/JlsJ (Stock No. 006178), CD9/JlsJ (Stock No. 006179) and CD10/Jls (Stock No. 006180) may serve as tools for mapping and characterizing genes responsible for estradiol sensitivity. .....
For more information please see the full phenotype on the strain data sheet
007042 D2.129P2(B6)-Nr5a1tm2Klp/EiJ
Repository-Cryopreserved
Mice homozygous for this floxed allele are viable and fertile. These mutant mice have loxP sites flanking the C-terminal coding exon. When bred to Cre-recombinase expressing mice, offspring will have a deletion of this exon in the cre expressing tissue(s). These floxed mice may be useful in studying steroidogenic factors and pituitary gonadotrope function.

For example, when crossed to a strain expressing Cre recombinase in the anterior and intermediate lobes of the pituitary gland (see Stock No. 004426), this mutant mouse strain may be useful in studies of pituitary gonadotrope function.

007483 FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
Repository-Cryopreserved
On an albino background Tg(Tyr)3412ARpw permits the identification of gender as early as embryonic day 10.5. This strain is segregating for Tg(Tyr)3412ARpw and homozygous for Tyrc-2J so the individuals not carrying Tg(Tyr3412)ARpw are albino. Because Tg(Tyr)3412ARpw inserted into the X Chromosome, breeding a carrier male with a noncarrier (wild-type) female results in embryos in which all XX individuals develop eye pigment, due to the Tg(Tyr)3412ARpw inherited from their father, while all XY individuals have non-pigmented eyes, having inherited a wild-type X Chromosome from their mother.

This strain is also homozygous for Tg(SryEGFP)92Ei. This reporter transgene consists of a 5' regulatory segment of the Sry gene driving EGFP. This transgene is expressed in the pre-support cell lineage (pre-sertoli and pre-granulosa cells) of the fetal genital ridge (Albrecht and Eicher, 2001) and in discrete areas the adult male but not female brain (Dewing et al., 2006 .....
For more information please see the full phenotype on the strain data sheet

002437 FVB/N-Tg(MMTV-Notch4)3Rnc/J
Repository-Cryopreserved
Male mice transgenic for the Notch4 gene (previously called Int3) are sterile, and females fail to lactate. Mammary tissue of females does not develop completely, exhibiting dramatic inhibition of alveolar-lobular development and reduced penetration of the mammary fat pad by ductal epithelium. Glandular epithelia of tissues expressing the activated form of Notch4 generally display severe ductal hyperplasia. Salivary glands fail to differentiate completely. Male transgenic mice exhibit severe epididymal hyperplasia, which is thought to be the cause of their sterility. Both male and female mice develop focal adenomas of the mammary and salivary glands.
003377 FVB/N-Tg(Zp3-cre)3Mrt/J
Repository-Cryopreserved
The strain originated on an FVB/N background and is currently on the same background. The investigator maintains the strain by breeding hemizygotes. Homozygotes are subfertile. This is a transgenic line in which cre expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene, which is normally expressed exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain would be useful for deleting a floxed sequence specifically in the female germ line.
005994 STOCK Mbtps1tm1Jdh/J
Repository-Cryopreserved
These mice carry a targeted mutation in which exon 2 of the targeted gene is flanked by loxP sites. A loxP-flanked ("floxed") neomycin resistance cassette also is inserted downstream in intron 2. Homozygotes are viable and fertile, and the floxed gene appears to function normally. When homozygotes are crossed with transgenic strains expressing Cre-recombinase, cre-mediated recombination of the loxP-flanked sequences can result in one of three genotypes: a) deletion of the neo cassette only, leaving a loxP-flanked second exon and unimpaired endogenous gene function. b) Deletion of exon 2 only, leaving a loxP-flanked neo cassette and no endogenous gene function. c) Deletion of both the neo cassette and exon 2, leaving a single loxP site and no endogenous gene function. When these floxed mutant mice are bred to mice carrying the Mx1-cre transgene (for example, Stock No. 003556), liver- .....
For more information please see the full phenotype on the strain data sheet
002858 STOCK Tg(Nes-cre)1Wme/J
Repository-Cryopreserved
These transgenic mice carry the Cre recombinase gene under the control of the rat nestin promoter. When crossed with a strain carrying a gene flanked by loxP sites, partial deletion of the loxP-flanked allele occurs before embryonic day 10.5 and is detectable in all adult organs examined including germ line cells. Because of the partial deletion, these balancer1 cre transgenic mice, as well as the balancer2 cre transgenic mice (Stock No. 002859), may be used to generate mosaic mice consisting of cells that are either wildtype or mutant for the gene of interest. The proportion of cells that carry the mutant allele varies between tissues and between individual mice. In cases where deletion of a particular gene is lethal, the generation of mosaic mice using these strains can bypass lethality in some mosaic individuals. The balancer2 line induces more variable deletion of loxP-flanked genes than the .....
For more information please see the full phenotype on the strain data sheet
002859 STOCK Tg(Nes-cre)2Wme/J
Repository-Cryopreserved
These transgenic mice carry the Cre recombinase gene under the control of the rat nestin promoter. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing cre, generating a proportion of mosaic animals. All adult organs, including germ-line cells, may be affected. The balancer2 line of transgenic mice show greater variability and instability in their generation of mosaics than the balancer1 line (Stock No. 002858). This variability of the balancer2 line can be of particular benefit if the gene of interest is vital and mosaic individuals are used to bypass lethality.
002395 STOCK Tg(Zfy1-lacZ)218Bri/J
Repository-Cryopreserved
LacZ staining is present in the embryonic gonad of both male and female mice (somatic cells, but not germ cells). Staining is less intense in males and is repressed shortly after formation of the testis cords. Staining is also present in the kidney, brain, and spine prior to birth.
003275 STOCK Tg(tetL)1Bjd/J
Repository-Cryopreserved
STOCK Tg(tetL)1Bjd/J transgenic mice are viable, fertile, and do not display an overt phenotype. These transgenic mice carry the luciferase gene coupled to a tetracycline-responsive promoter element (TRE). When mated to a strain expressing the tetracycline-controlled activator proteins tTA or rtTA coupled to a tissue-specific promoter, luciferase will be expressed in the appropriate tissue in a tetracycline-inducible fashion.
003274 STOCK Tg(tetNZL)2Bjd/J
Repository-Cryopreserved
In this strain both the lacZ gene with a nuclear localization signal (NZ) and the luciferase gene (L) are driven by a bidirectional, tetracycline-responsive promoter element (TRE). When mated to a strain expressing the tetracycline-controlled activator proteins tTA or rtTA coupled to tissue-specific promoters, lacZ and luciferase will be expressed in the appropriate tissue in a tetracycline-inducible fashion. According to the donating investigator, the expression of the two reporter genes is biased toward higher levels of lacZ than of luciferase, which, although low, is still measurable.

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New Strains Under Development

(See informational text following listing of strains)
How to Register Interest
Please indicate your interest in purchasing any of the strains listed below when they become available for distribution by checking the box next to the strain(s) of interest and then selecting the "Continue" button which leads to an Interest Form.

View a Data sheet for New Strains Under Development
Select the strain name to link to the strain data sheet.

Stock
Number
Strain Name
 
Phenotype
Standard Supply
003208STOCK Tg(DR4)1Jae/J
On Hold
MEFs prepared from the DR-4 mouse strain displayed resistance to concentrations of the drugs G418, 6-thioguanine, puromycin and hygromycin well above those used normally for the selection of drug-resistant ES cells. This mouse strain represents an economical donor for the production of multiple-drug resistant mouse embryonic fibroblasts (MEFs).
008569129-Alpltm1(cre)Nagy/J
Under Development for Production
This strain expresses Cre recombinase from the targeted locus. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in tissue-specific deletion of the flanked sequence. Recombination occurs primarily in embryonic primordial germ cells. Approximately 60% of gonadal cells isolated from embryonic day 13.5 embryos exhibit Cre recombinase activity. Mosaic ectopic recombinase activity does occur. Homozygotes are not viable. This mutant mouse strain represents a model that may be useful in studies of reproductive and endocrine systems development.
006965B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
Under Development for Production
Homozygotes are viable, fertile, normal in size and do not display any behavioral abnormalities. These targeted mutant mice have widespread expression of an optimized form of reverse tetracycline-controlled transactivator (rtTA-M2) protein. This R26-M2rtTA strain may be useful for doxycycline-inducible studies which utilize rtTA/tet-O (tet-on/TRE) models.
008042B6;129P2-Adam2tm1Dgm/J
Under Development for Production
Mice homozygous for this fertilin b (Adam2) mutant allele are viable with no gross phenotypic abnormalities reported. Both precursor and processed fertilin b proteins were absent from spermatogenic cells and mature sperm isolated from homozygous males. While homozygous females are fertile with normal egg activation, homozygous males exhibit sperm deficiencies in sperm-egg membrane adhesion, sperm-egg fusion, migration from the uterus into the oviduct, and binding to the egg zona pellucida, rendering them infertile. These fertilin b (Adam2) mutant mice may be useful in reproductive biology studies; specifically to determine the role of ADAM (A Disintegrin And Metalloprotease) family proteins in sperm-egg interactions, fertilization, and spermatogenesis. These mice may also be useful in conjunction with other ADAM family mutant mice, including the cyritestin (Adam3)-deficient strain ( .....
For more information please see the full phenotype on the strain data sheet
008043B6;129P2-Adam3tm1Pmkf/J
Under Development for Production
Mice homozygous for this cyritestin (Adam3) mutant allele are viable with no gross phenotypic abnormalities reported. No protein product is detected from the targeted gene in sperm isolated from homozygous males. While homozygous females are fertile, homozygous males exhibit sperm deficiencies in adhesion to the egg zona pellucida and to the egg plasma membrane rendering them infertile. These cyritestin (Adam3) mutant mice may be useful in reproductive biology studies; specifically to determine the role of ADAM (A Disintegrin And Metalloprotease) family proteins in sperm-egg interactions, fertilization, and spermatogenesis. These mice may also be useful in conjunction with other ADAM family mutant mice, including the fertilin b (Adam2)-deficient strain (see Stock No. 008042).
007900C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J
Under Development for Production
Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.

For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation.

008119STOCK Tg(Neurog3-cre/Esr1)1Dam/J
Under Development for Production
These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse neurogenin 3, Neurog3, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in islet cells of the pancreas, undifferentiated spermatogonia and other Neurog3 expressing cells. Transgene expression closely patterns endogenous gene expression as analyzed by in situ hybr .....
For more information please see the full phenotype on the strain data sheet
008168STOCK Tg(tetO-DTA)1Gfi/J
Under Development for Production
These tet-DTA transgenic mice express diphtheria toxin A (DTA) under the control of a tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) and a cytomegalovirus minimal promoter. When bred with another mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue diphtheria toxin A expression can be regulated with the tetracycline analog doxycycline (dox) in the resulting double mutant offspring. These tet-DTA mice may be useful in generating bi-transgenic mutant mice for the reversible, inducible deletion of specific groups of cells.

For example, when bred to a strain expressing tTA in cardiac myocytes (see Stock No. 003170 for example), this mutant mouse strain may be useful in studies of human cardiomyopathies.

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New Strains Under Development The Jackson Laboratory serves as a worldwide distributor and national repository for common and rare strains of inbred mice and mice carrying spontaneous mutations or induced mutations (i.e., transgenic, targeted/"knockout", or chemically induced mutations). At any one time, we have over 100 strains at various stages of development and colony expansion. Strains "Under Development" fall into two categories depending on the anticipated demand from the scientific community.
  1. Strains that will be made available from a live distribution colony at The Jackson Laboratory.
    These strains are designated as: "Under Development for Distribution Colony"
  2. Strains that will be made available through the Cryopreservation Repository.
    These strains are designated as: "Under Development for Cryopreservation Repository"
It is VERY IMPORTANT that you register interest in strains Under Development. The anticipated demand for a strain enables us to determine effectively the distribution plan for each strain Under Development. Registering interest also provides benefits to you (including advance notification of pending availability). Whether a strain is made available from a live colony OR from our cryopreservation repository, you may want to consider the option of Dedicated Supply. To learn more about Dedicated Supply, go to Services.

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