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| Stock Number |
Strain Name Strain Description |
Standard Supply |
| 002468 | KK.Cg-Ay/J | Level 3 |
| Ay and other mutations at the a locus conferring a completely yellow coat color are dominant to all a alleles that produce a darker coat. Hair pigment of Ay heterozygotes is yellow, but eyes are black. Heterozygotes usually become obese and infertile within a few months after birth. Increased adipose tissue mass is due to fat cell hypertrophy, and it has been hypothesized that the obesity results from the observed reduction in hypothalamic norepinephrine and dopamine. Heterozygotes are more susceptible to several kinds of tumors than are normal mice, possibly due, at least in part, to a general increase in cell proliferation that also manifests as a slight increase in lean body mass and skeletal length. Further spleen cells from heterozygotes cause a significantly lower graft vs. host reaction. Mice homozygous for the yellow spontaneous mutation (Ay) die before implantation, or shortly thereafter. The time of death and ..... For more information please see the full phenotype on the strain data sheet | ||
| 003291 | C57BL/6-Tg(CAG-EGFP)1Osb/J | Level 4 |
| This transgenic mouse line with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer makes all of the tissues, with the exception of erythrocytes and hair, appear green under excitation light. Note that the donating investigator reports that mice homozygous for this transgene die within the first two weeks following birth.
Of note, it has been the experience at The Jackson Laboratory that Stock No. 006567 (C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ) demonstrates the highest proportion of GFP expressing cell populations in bone marrow, thymus, spleen and peripheral blood when compared to Stock No. 003291 (C57BL/6-Tg(CAG-EGFP)1Osb/J) and Stock No. 007075 (CByJ.B6-Tg(CAG-EGFP)1Osb/J).
View the pdf document on For more information please see the full phenotype on the strain data sheet | ||
| 008569 | 129-Alpltm1(cre)Nagy/J | Repository- Live |
| This strain expresses Cre recombinase from the targeted locus. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in tissue-specific deletion of the flanked sequence. Recombination occurs primarily in embryonic primordial germ cells. Approximately 60% of gonadal cells isolated from embryonic day 13.5 embryos exhibit Cre recombinase activity. Mosaic ectopic recombinase activity does occur. Homozygotes are not viable. This mutant mouse strain represents a model that may be useful in studies of reproductive and endocrine systems development. | ||
| 016567 | 129S.Cg-Tg(Hoxb7-rtTA*M2)2Cos/J | Repository- Live |
| RS-HTA2 transgenic mice have the homeobox B7 promoter/enhancer sequences driving expression of an optimized form of the reverse tetracycline-controlled transactivator (rtTA*M2) protein. Hemizygotes are viable, fertile, and normal in size. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the gene of interest may be regulated by the tetracycline analog, doxycycline (dox). In the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. When bred to B6;SJL-Tg(tetop-lacZ)2Mam/J mice (Stock No. 002621), adult mice carrying both transgenes, which were maintained on Dox during pre and postnatal life, show strong expression of βgal in the renal collecting duct system, and embryos display strong expression throughout the Wolffian duct, ureteric bud, vas deferens, epididymis ..... For more information please see the full phenotype on the strain data sheet | ||
| 007915 | 129S.FVB-Tg(Amh-cre)8815Reb/J | Repository- Live |
| Mice harboring the Amh-cre transgene are viable and fertile, with expression of Cre recombinase directed by the testis Sertoli cell-specific promoter elements of the anti-Mullerian hormone (Amh) gene. Cre-recombinase activity is reported in testis Sertoli cells during male sexual development as early as E14.5, with no evidence for cre expression detected in other tissues examined. When Amh-cre transgenic males are bred with female mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence specifically in testis Sertoli cells. These Amh-cre transgenic mice may be useful in generating conditional knockouts in testis Sertoli cells for studying male embryonic sexual differentiation and the regulation of spermatogenesis. | ||
| 008471 | B6.129(SJL)-Oxtrtm1.1Wsy/J | Repository- Live |
| Mice homozygous for the Oxtrflox allele are viable and fertile, with loxP sites flanking exons 2-3 of the targeted gene. Expression and receptor binding distributions from the Oxtrflox targeted allele are reported to be normal when compared to wild-type. When Oxtrflox mice are bred to mice that express Cre recombinase, the resulting offspring will have the floxed region deleted in the cre-expressing tissue(s). These Oxtrflox mice may be used for spatial and temporal inactivation of the oxytocin receptor in studying (for example) parturition and lactation, as well as social, behavioral, and learning disorders such as autism and anxiety.
These Oxtrflox mice may also be useful along with the Oxt/EGFP AI03 transgenic mice (Stock No. 006043) or oxytocin targeted mutant mice (Stock No. 002713) from the same ..... | ||
| 007741 | B6.129-Arg1tm1Rki/J | Repository- Live |
| Homozygous AI-mutant mice completely lack hepatic arginase (AI) activity, exhibit hyperagrininemia, severe symptoms of hyperammonemia (ncluding decerebrate posture, lethargy, and high-frequency tremor of the extremities, particularly the tail) and die between 10-14 days after birth. Neural stem cells (NCSs) isolated from homozygous mice exhibit abnormal proliferation and differentiation. In addition, haploid germ cells carrying the disrupted AI allele may be less fit/less effective in forming zygotes compared to wild-type spermatozoa. Heterozygotes are viable and fertile. These AI-mutant mice may be useful in studying metabolic defects of arginase I deficiency, urea cycle (excretion of excess nitrogen), and neuronal development and function. | ||
| 005867 | B6.129-Ido1tm1Alm/J | Repository- Live |
| Homozygous mice are viable and fertile with normal immune system development and function. They exhibit no spontaneous autoimmune disorders. No gene product (mRNA or protein) from the targeted gene is detected in the epididymis. At embryonic day 10.5, endogenous protein is absent from all cells at the maternal-fetal interface when both parents are homozygous for the targeted gene. Allogeneic and syngeneic pregnancy outcomes are unaffected by this mutation. In contrast to wild-type, anti-proliferative treatments (CTLA4-Ig, IFNalpha, or CpG-ODN) do not suppress T cell expansion both in vivo and in vitro. In addition, homozygous dendritic cells isolated from lymph nodes draining (induced) tumor sites have no suppressor activity. These mice may be useful in studies of pregnancy and reproductive immunology (tryptophan degradation, T cell activation, clonal expansion) as well as autoimmune disease, tissue transplantation, fostering, acquired tolerance/T cell anergy, and immunos ..... For more information please see the full phenotype on the strain data sheet | ||
| 011029 | B6.129-Rpl22tm1.1Psam/J | Repository- Live |
| Mice homozygous for this RiboTag allele are viable and fertile, with a targeted mutation of the ribosomal protein L22 (Rpl22) locus harboring a loxP-flanked wildtype C-terminal exon 4 followed by an identical C-terminal exon 4 that is tagged with three copies of the hemagglutinin (HA) epitope before the stop codon. Prior to exposure to Cre recombinase, RiboTag mice express the wildtype RPL22 protein (15 kDa). When the RiboTag mice are bred to cre-expressing mice, offspring will have the floxed wildtype exon 4 deleted in the cre-expressing tissue and subsequent use of the downstream HA epitope-tagged exon 4 as the terminal exon. The 23 kDa HA epitope-tagged ribosomal protein (RPL22HA) is incorporated into actively translating polyribosomes. These RiboTag mice allow Cre-mediated HA epitope tagging of ribosomes from user-defined cell types and/or immunoprecipitation of ribosomes bound to mRNA from those specific cell types. | ||
| 011008 | B6.129P2(Cg)-Gt(ROSA)26Sortm1(tTA)Roos/J | Repository- Live |
| Mice heterozygous for the ROSA:LNL:tTA conditional mutation of the Gt(ROSA)26Sor locus are viable and fertile, with a loxP-flanked STOP cassette preventing transcription of a downstream optimized/modified tetracycline-controlled transactivator protein ("mtTA"). Applying both Cre-lox and Tet-Off technologies, these ROSA:LNL:tTA mutant mice may be useful to generate compound mutant mice in which expression of a tetracycline-responsive promoter element (TRE; also called tet-operator or tetO)-driven gene of interest can be both directed to the cell types defined by the chosen Cre recombinase expression, as well as turned off by the addition of tetracycline (or its analog doxycycline (dox)). | ||
| 013584 | B6.129P2-Hspa2tm1Dix/J | Repository- Live |
| Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous male mice are infertile, homozygous female mice are fertile. No gene product (mRNA) is detected by Northern blot analysis of testis tissue from homozygous male animals. Immunohistochemical examination of seminiferous tubules does not detect protein gene product. Homozygous males have testes that weigh significantly less than testes from controls. Spermatogenesis in homozygotes is arrested in prophase of meiosis I with a reduced number of postmeitotic spermatids in seminiferous tubules, many with abnormal morphology, and no spermatozoa in the epididymis. Apoptosis of germ cells (late pachytene spermatocytes) in the testes is greatly increased in mutants. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background. | ||
| 016222 | B6.129S(Cg)-Id2tm1.1(cre/ERT2)Blh/ZhuJ | Repository- Live |
| The Id2-CreERT2 knockin allele was designed to both abolish inhibitor of DNA binding 2 (Id2) gene function and express CreERT2 fusion protein from the Id2 promoter/enhancer elements. Cre-ERT2 fusion gene activity is inducible and can be observed following tamoxifen administration. As such, when Id2-CreERT2 knockin mice are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in the Id2-expressing cells of the offspring.
No mRNA or protein expression from the Id2-CreERT2 allele is observed. The donating investigator reports that homozygous mice are runted with defective lung alveolarization. Other organ systems have not been evaluated. However, Id2-CreERT2 homozygotes may be expected to exhibit the same phenotype as mice homozygous for other null mutations of this gene (including postnatal lethality and defects of the immune system, digestive tract, ..... | ||
| 006233 | B6.129S1-Casp3tm1Flv/J | Repository- Live |
| On this C57BL/6 congenic background, homozygotes are viable, fertile, and reach adulthood, but females reported display suboptimal mothering instincts. Functional endogenous protein and mRNA are absent from all tissues tested. Homozygous mice are resistant to in vivo cerebral ischemia/reperfusion and in vitro oxygen-glucose deprivation. Ovaries from female homozygotes show aberrant atretic follicles associated with a granulosa cell-intrinsic defect in apoptosis as well as defective corpus luteum regression. Homozygous mice are congenitally deaf with hair cell defects in the Organ of Corti. Optic lens formation/morphology also is abnormal with cataracts at the anterior lens pole. Of note, these mice lack the embryonic/perinatal-lethal brain pathology observed in mutant mice on the 129 and mixed B6;129 genetic backgrounds. These mutant mice may be useful in studies of apoptosis, ovarian follicle and corpus luteum development, and eye and ear development. | ||
| 007668 | B6.129S4(Cg)-Arntltm1Weit/J | Repository- Live |
| Mice homozygous for this conditional (floxed) allele possess loxP sites flanking exon 8 of the targeted gene and are viable and fertile, with circadian behavioral rhythms indistinguishable from wildtype littermates. When bred to mice that express Cre recombinase, the resulting offspring will have the exon encoding the ARNTL (BMAL1) basic helix-loop-helix (bHLH) domain deleted in the cre-expressing tissue(s). These Bmal1-floxed mutant mice may be useful in generating conditional mutations (whole-mouse or tissue-specific) to study the role of circadian clock/circadian rhythm in physiological and behavioral regulation.
For example, when crossed to a strain expressing Cre recombinase in the retina (see Stock No. 005105), this mutant mouse strain may be useful in studies of the circadian clock of the retina. | ||
| 007899 | B6.129S4-Casp2tm1Yuan/J | Repository- Live |
| Mice homozygous for this caspase-2 targeted mutation are viable and fertile. As the mutation deletes the QACRG active site and the caspase-2S sequence of the endogenous enzyme, this deletion was shown to inactivate both the long and short form of caspase-2. As such, homozygous mice exhibit defects in regulation of apoptosis; including an enlarged oocyte reserve attributed to a germ cell-intrinsic death defect during prenatal ovarian development (resistance to oocyte cell death following complete cytokine starvation or exposure to an anticancer drug), as well as accelerated motor neuron cell death and defective B lymphoblast apoptosis. In addition, caspase-2-deficient mice exhibit characteristics of premature aging (including shortened maximum lifespan, impaired hair growth, increased bone loss, reduced body fat content, and higher hepatic levels of oxidized proteins). As caspase-2 acts as an upstream regulator of cell death in many cell types, caspase-2-deficient mice may b ..... For more information please see the full phenotype on the strain data sheet | ||
| 011009 | B6.129S4-Mtortm1.2Koz/J | Repository- Live |
| Mice homozygous for this mTORfl allele are viable and fertile, with loxP sites flanking exons 1-5 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the putative transcription start site and the coding sequence through coding exon 5 deleted in the cre-expressing tissue(s). These mTORfl mutant mice may be useful in generating conditional mutations for studying mTOR and mTOR complex signaling; including regulation of cell growth, cell proliferation/differentiation, cell survival, cancer, tumor cell motility and metastasis, the RTK-PI3K-mTOR signaling axis, stem cell development, cytokine signaling, and T cell lineage commitment.
For example, when bred to a strain expressing Cre recombinase in adult striated muscle fibers and embryonic striated muscle cells of the somites and heart (see Stock No. 006149 for example), ..... | ||
| 012565 | B6.129S7(129S4)-Ift20tm1.1Gjp/J | Repository- Live |
| These Ift20flox mice harbor loxP sites flanking exons 2-3 (encoding the first 71 codons including the start codon) of the intraflagellar transport 20 homolog (Chlamydomonas) locus. The primary cilium is a microtubule-based antenna-like structure that emanates from the surface of virtually all cells in the mammalian body. The primary cilium functions as a sensory organelle (mechano-, chemo-, photo-receptor) that receives signals from other cells/the environment, and transmits these signals to the nucleus to elicit a cellular response. Most types of eukaryotic cilia and flagella are assembled and maintained by the process of intraflagellar transport (IFT). During IFT, large protein complexes (IFT particles) are transported along the ciliary microtubules under the ciliary membrane. IFT particle proteins organize into at least three distinct complexes called complex A, complex B and the Golgi IFT complex. The unique role of Ift20 in both complex B as well as the Golgi IFT ..... For more information please see the full phenotype on the strain data sheet | ||
| 002192 | B6.129S7-Gt(ROSA)26Sor/J | Repository- Live |
| Mice heterozygous or homozygous for the ROSA26 retroviral insertion display no distinguishing phenotype. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult mouse.
If breeding heterozygous mice together, recovery of homozygous offspring is significantly reduced. For unknown reasons, homozygous mutant mice are prone to convulsions. If breeding or creating homozygous mice, they should be handled carefully and quietly to avoid poor breeding, loss of litters, or premature death. | ||
| 000021 | B6.Cg-Ay/J | Repository- Live |
| Mice homozygous for the yellow spontaneous mutation (Ay) die before implantation or shortly thereafter. The time of death and type of abnormality is, in part, determined by the genetic background on which the mutation is placed. Hair pigment in heterozygous mice is yellow, but eyes are black. Heterozygotes usually become obese and infertile after the first few months. Increased adipose tissue mass is due to fat-cell hypertrophy. It has been hypothesized that the obesity results from the observed reduction in hypothalamic norepinephrine and dopamine levels. Insulin resistance and hyperglycemia follow development of hyperinsulinemia in early adulthood, although the degree is less severe than on the KK/UpJ genetic background (Stock No. 002468). Heterozygotes are also more susceptible to several kinds of tumors than normal mice, and their spleen cells cause a significantly lower graft vs. host reaction. The level of ..... For more information please see the full phenotype on the strain data sheet | ||
| 006965 | B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J | Repository- Live |
| Homozygotes are viable, fertile, normal in size and do not display any behavioral abnormalities. These targeted mutant mice have widespread expression of an optimized form of reverse tetracycline-controlled transactivator (rtTA-M2) protein. This R26-M2rtTA strain may be useful for doxycycline-inducible studies which utilize rtTA/tet-O (tet-on/TRE) models. | ||
| 007914 | B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | Repository- Live |
| Ai14 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE::deltaNeo conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. Ai14 mice are not expected to express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre is expected to be detectable by fluorescence and mRNA (in situ hybridization). These Ai14 mice are useful as a Cre reporter strain; expressing the red fluorescent protein variant, tdTomato, following Cre-mediated recombination.
For cha ..... | ||
| 012567 | B6.Cg-Gt(ROSA)26Sortm27.1(CAG-COP4*H134R/tdTomato)Hze/J | Repository- Live |
| Ai27D (or Ai27Δneo) mice heterozygous for the Rosa-CAG-LSL-hChR2(H134R)-tdTomato-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream hChR2(H134R)-tdTomato fusion gene. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, hChR2(H134R)-tdTomato expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the hChR2(H134R)-tdTomato fusion protein. The donating investigator reports that Ai27D mice do not express hChR2(H134R)-tdTomato prior to introduction of Cre recombinase. Fusion protein expression following exposure to cre can be detected by tdTomato fluorescence and mRNA (in situ hybridization) (and presumably by antibody staining (immunohistochemistry); althoug ..... For more information please see the full phenotype on the strain data sheet | ||
| 007903 | B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J | Repository- Live |
| Ai3 mice hemizygous for this Rosa-CAG-LSL-EYFP-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream enhanced yellow fluorescent protein (EYFP). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of EYFP. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, EYFP expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai3 mice do not express EYFP prior to introduction of Cre recombinase and EYFP expression following exposure to cre can be detected by fluorescence, mRNA (in situ hybridization) and antibody staining (immunohistochemistry). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-medi ..... For more information please see the full phenotype on the strain data sheet | ||
| 007906 | B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J | Repository- Live |
| Ai6 mice hemizygous for this Rosa-CAG-LSL-ZsGreen1-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream enhanced green fluorescent protein (ZsGreen1). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of ZsGreen1. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, ZsGreen1 expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai6 mice do not express ZsGreen1 prior to introduction of Cre recombinase and ZsGreen1 expression following exposure to cre can be detected by fluorescence and mRNA (in situ hybridization). Bright fluorescence is observed mainly in cell bodies. Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the report ..... For more information please see the full phenotype on the strain data sheet | ||
| 007909 | B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J | Repository- Live |
| Ai9 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai9 mice do not express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre can be detected by fluorescence and mRNA (in situ hybridization). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-mediated recombination if ..... For more information please see the full phenotype on the strain data sheet | ||
| 007484 | B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ | Repository- Live |
| On an albino background the X-linked transgene Tg(Tyr)3412ARpw permits visual identification of XX versus XY as early as embryonic day 10.5. This strain is segregating for Tg(Tyr)3412ARpw and homozygous for Tyrc-2J so the individuals not carrying Tg(Tyr3412)ARpw are albino. Because Tg(Tyr)3412ARpw inserted into the X Chromosome, breeding a carrier male with a noncarrier (wild-type) female results in embryos in which all XX individuals develop eye pigment, due to the Tg(Tyr)3412ARpw inherited from their father, while all XY individuals have non-pigmented eyes, having inherited a wild-type X Chromosome from their mother.
This strain is also homozygous for Tg(SryEGFP)92Ei. This reporter transgene consists of a 5 prime regulatory segment of the Sry gene driving EGFP. This transgene is expressed in the pre-support cell lineage (pre-sertoli and pre-granulosa cells) of the fetal genital ridge (Albrecht and Eicher, 2001) and in discrete areas the adult male but not ..... | ||
| 006881 | B6.Cg-Tg(Aqp2-cre)1Dek/J | Repository- Live |
| Mice hemizygous for this AQP2-Cre transgene are viable and fertile. Transgenic cre activity, directed by the mouse aquaporin 2 promoter, is observed in kidney cells (collecting duct) and testes (sperm). When bred with mice containing a loxP-flanked sequence of interest, cre-mediated recombination will result in deletion of the flanked sequence. In such breedings, maternal inheritance of the transgene is recommended for kidney-specific recombinase activity as males express cre in sperm as well as kidney tissues. These AQP2-Cre mice may be used to generate conditional mutations in the renal collecting duct for studying nephrology, physiology, metabolism, or type II diabetes. In addition, cre expression in sperm may be useful in generating conditional mutations in multiple or all tissues in the resulting offspring. | ||
| 012237 | B6.Cg-Tg(Cdh16-cre)91Igr/J | Repository- Live |
| Hemizygous and homozygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These Ksp1.3/Cre transgenic mice express Cre recombinase under the control of the mouse cadherin 16 (Cdh16 or Ksp-cadherin) promoter. Cre recombinase expression follows expression of the endogenous gene and is detected in the epithelial cells of developing nephrons, ureteric bud, mesonephric tubules, Wolffian duct, and Mullerian duct. In the adult mouse expression is limited to the renal tubules especially the collecting ducts, loops of Henle and distal tubules. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in the offspring. This mutant mouse strain may be useful in kidney-specific gene targeting and cell lineage studies. | ||
| 007897 | B6.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J | Repository- Live |
| Mice hemizygous for this ROSA26-EGFP BAC transgene are viable and fertile, with widespread EGFP expression in all organs and tissues analyzed. In addition, stable and uniform EGFP expression is observed in differentiated progeny of transplanted hematopoietic stem cells (HSC) isolated from ROSA26-EGFP mice. These ROSA26-EGFP BAC transgenic mice may be useful for fluorescent labeling of cells and tissues both in vivo and in vitro, are suitable for use in histological preparations, and are appropriate as a source of fluorescently labeled donor cell populations (such as tracking hematopoietic cell fate in transplant recipients).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as publis ..... | ||
| 010905 | B6.Cg-Tg(Sry)2Ei Srydl1Rlb/ArnoJ | Repository- Live |
| The dl1Rlb allele (Y-) is an 11 kb deletion in the sex determining region of the Y chromosome, Sry , XY- mice (with ovaries) with this mutation are phenotypic gonadal females, although they lose germ cells and cease estrous cycling earlier in life. The donating investigator indicates that XY- mice generally infertile on the C57BL/6 background . XX mice carrying the Tg(Sry)2Ei transgene are phenotypic gonadal males (with testes), although they lack sperm and have smaller testes than normal males.
When the two mutations are combined, testis determination is transferred from the Y chromosome to an autosome. Mating the carrier male to a C57BL/6J female produces four "core" genotypes that can be used as a model to investigate relationships between sex chromosome complement (XX or XY) and gonadal type that influences phenotypic characteristics. The four genotypes produced are two types of gonadal females (XX, XY-), and two types ..... For more information please see the full phenotype on the strain data sheet | ||
| 008468 | B6.Cg-Tg(tetO-DTA)1Gfi/J | Repository- Live |
| These tet-DTA transgenic mice express diphtheria toxin A (DTA) under the control of a tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) and a cytomegalovirus minimal promoter. When bred with another mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue diphtheria toxin A expression can be regulated with the tetracycline analog doxycycline (dox) in the resulting double mutant offspring. These tet-DTA mice may be useful in generating bi-transgenic mutant mice for the reversible, inducible deletion of specific groups of cells.
For example, when bred to a strain expressing tTA in cardiac myocytes (see Stock No. 003170 for example), this mutant mouse strain may be useful in studies of human cardiomyopathies. When bred to a strain expressing tTA in pancreatic beta cells (see Stock N ..... | ||
| 015854 | B6;129P2-Foxl2tm1(GFP/cre/ERT2)Pzg/J | Repository- Live |
| These Fox L2-GCE knock in mice utilize a tamoxifen inducible Cre-mediated recombination system. A GFP-Cre/ERT2 cassette (GCE) was inserted into the Foxl2, forkhead box L2, locus. No green fluorescence was detected by direct fluorescence microscopy, however, immunohistochemical analysis revealed GFP expression in embryos, 15.5 embronic days of age. Tamoxifen administration induces Cre recombination in a small population of granulosa precursor cells in the medulla of the developing ovary of embryos, 15.5 embronic days of age. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 012336 | B6;129P2-Terf1tm2.1Tdl/J | Repository- Live |
| These TRF1F mice harbor loxP sites flanking exon 1 (encoding the translation start site) of the telomeric repeat binding factor 1 locus. Telomeres serve a dual role in protecting the chromosome ends from degradation/repair activities and in intracellular signaling for regulating cell proliferation. Mammalian telomeres are formed by tandem TTAGGG sequence repeats bound by a specialized complex of six telomere-associated proteins called the shelterin complex. As TRF1 is one of the components of shelterin, these TRF1F mutant mice may be useful in generating conditional mutations for studying the shelterin complex of telomeres, telomere maintenance, chromosomal stability, cancer, and aging. | ||
| 012569 | B6;129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J | Repository- Live |
| Ai32 mice heterozygous for the Rosa-CAG-LSL-ChR2(H134R)-EYFP-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream ChR2(H134R)-EYFP fusion gene. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, ChR2(H134R)-EYFP expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the ChR2(H134R)-EYFP fusion protein. The donating investigator reports that Ai32 mice do not express ChR2(H134R)-EYFP prior to introduction of Cre recombinase. Fusion protein expression following exposure to cre can be detected by EYFP fluorescence (and presumably by mRNA (in situ hybridization) and antibody staining (immunohistochemistry); although this was not tested by the donating investigator). ..... For more information please see the full phenotype on the strain data sheet | ||
| 012570 | B6;129S-Gt(ROSA)26Sortm34.1(CAG-Syp/tdTomato)Hze/J | Repository- Live |
| Ai34D (or Ai34Δneo) mice heterozygous for the Rosa-CAG-LSL-Synaptophysin-tdTomato-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream Synaptophysin-tdTomato fusion gene. The Synaptophysin-tdTomato fusion protein is composed of near-full-length mouse synaptophysin protein fused in-frame to the amino terminus of the tdTomato fluorescent protein. Because the CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, Synaptophysin-tdTomato expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the Synaptophysin-tdTomato fusion protein. The donating investigator reports that Ai34D mice do not express Synaptophysin-tdTomato prior to introduction of Cre recombinase. Following exposure to ..... For more information please see the full phenotype on the strain data sheet | ||
| 012735 | B6;129S-Gt(ROSA)26Sortm35.1(CAG-AOP3/GFP)Hze/J | Repository- Live |
| Ai35D (or Ai35Δneo) mice heterozygous for the Rosa-CAG-LSL-Arch-GFP-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream Arch-GFP fusion gene (see below for detailed description of Arch-GFP). Because the CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, Arch-GFP expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the Arch-GFP fusion protein.
The donating investigator reports that Ai35D mice do not express Arch-GFP prior to introduction of Cre recombinase. Fusion protein expression following exposure to cre can be detected by GFP fluorescence and mRNA (in situ hybridization) [and presumably by antibody staining (immunohistochemistry); although this was not test ..... For more information please see the full phenotype on the strain data sheet | ||
| 014538 | B6;129S-Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J | Repository- Live |
| Ai38 mice heterozygous for the Rosa-CAG-LSL-GCaMP3-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream GCaMP3 fusion gene (see below for detailed description of GCaMP3). Because the CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, GCaMP3 expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the fluorescent calcium indicator protein, GCaMP3. The donating investigator reports that Ai38 mice do not express GCaMP3 prior to introduction of Cre recombinase. Following exposure to Cre recombinase, GCaMP3 expression (EGFP fluorescence) is detected in the cre-expressing tissues. In the absence of calcium binding, low EGFP fluorescence is reported. Following calcium binding (s ..... For more information please see the full phenotype on the strain data sheet | ||
| 014539 | B6;129S-Gt(ROSA)26Sortm39(CAG-HOP/EYFP)Hze/J | Repository- Live |
| Ai39 mice heterozygous for the Rosa-CAG-LSL-eNpHR3.0-EYFP-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream eNpHR3.0-EYFP fusion gene (see below for detailed description of eNpHR3.0-EYFP). Because the CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, eNpHR3.0-EYFP expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the eNpHR3.0-EYFP fusion protein. The donating investigator reports that Ai39 mice do not express eNpHR3.0-EYFP prior to introduction of Cre recombinase.
Fusion protein expression following exposure to cre can be detected by EYFP fluorescence and mRNA (in situ hybridization) [and presumably by antibody staining (immunohistochemistry); although this was ..... For more information please see the full phenotype on the strain data sheet | ||
| 010983 | B6;129S-Id3tm1Pzg/J | Repository- Live |
| These mutant mice express Red Fluorescent Protein from the endogenous Id3 (inhibitor of DNA binding 3) locus. Strong red fluorescence is observed in the kidney. Red fluorescence is also detected in the urogenital system of E15.5 heterozygous embryos. Fluorescence mimics the endogenous expression pattern. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous.
This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 010987 | B6;129S-Sox18tm1(GFP/cre/ERT2)Pzg/J | Repository- Live |
| These Sox18-GCE mutant mice have a tamoxifen inducible Cre-mediated recombination system. A GFP-Cre/ERT2 cassette (GCE) was inserted into the Sox18, SRY-box containing gene 18, locus. Tamoxifen administration induces Cre recombination. Green fluorescence is detected in the kidneys and ovary of E15.5 heterozygous embryos. Cre recombinase activity is detected in the kidney from E15.5 heterozygous embryos. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are viable and fertile. This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 009389 | B6;129S1-Bambitm1Jian/J | Repository- Live |
| Mice homozygous for this Bambiflox allele are viable and fertile, with loxP sites flanking exon 1 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the endogenous signal peptide deleted in the cre-expressing tissue(s); this is expected to produce a null allele. These mutant mice may be useful in generating conditional mutations for studying the role of Bambi in developmental biology and the TGF-beta pathway. | ||
| 014592 | B6;129S4-Col1a1tm1(tetO-mCherry)Eggn/J | Repository- Live |
| These Col1a1-tetO-H2B-mcherry mutant mice contain a tet-responsive element (tetO) and a histone H2B-mCherry fusion protein, targeted to the collagen, type I, alpha 1 (Col1a1) locus. Homozygous are viable, fertile, and normal is size. When bred with another mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), expression of mCherry can be regulated with the tetracycline analog doxycycline (dox) in the resulting double mutant offspring.
Specifically, breeding Col1a1-tetO-H2B-mcherry mice with R26-rtTA mice (see Stock No. 006965) results in double mutant mice which express mCherry in embryonic stem (ES) cells, and differentiated trophoblast stem (TS) cells after administration of dox. These mice may be useful for visualizing pluripotent cells. | ||
| 007908 | B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | Repository- Live |
| Ai14 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE::deltaNeo conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. Ai14 mice are not expected to express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre is expected to be detectable by fluorescence and mRNA (in situ hybridization). These Ai14 mice are useful as a Cre reporter strain; expressing the red fluorescent protein variant, tdTomato, following Cre-mediated recombination.
The Allen I ..... | ||
| 007905 | B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J | Repository- Live |
| Ai9 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai9 mice do not express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre can be detected by fluorescence and mRNA (in situ hybridization). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-mediated recombination if ..... For more information please see the full phenotype on the strain data sheet | ||
| 004654 | B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein under the control of the POU domain, class 5, transcription factor 1, promoter and distal enhancer. Primordial germ cell specific markers, alkaline phosphatase II and stage-specific embryonic antigen, are co-expressed in EGFP positive cells. 9.5 and 10.5 dpc (days post-coitum) migratory primordial germ cells from hemizygotes and homozygotes can be sorted and isolated by flow cytometry. This strain represents an effective tool for studying genetic imprinting and early embryonic development. | ||
| 015853 | B6;DBA-Tg(Cited1-TagRFP)26Amc/J | Repository- Live |
| These transgenic mice express the Tag-RFPT variant of Red Fluorescent Protein under the direction of the mouse Cited1, Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 1, promoter. TagRFP is the optimized monomeric derivative of the tetrameric eqFP578 from the sea anemone, Entacmaea quadricolor.
RFP fluorescence is detected in the cap mesenchyme in the kidney and the male reproductive systems of hemizygous embryos, 15.5 embryonic days of age. Immunohistochemical analysis reveals that the TagRFP-T transgene is expressed in the expected Cited1 expression domain. Other possible sites of expression have not been characterized. Mice that are hemizygous for the knock in allele are viable, fertile, normal in size and do not display physical or behavioral abnormalities.
This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 014159 | B6;DBA-Tg(Tmem100-EGFP/cre/ERT2)30Amc/J | Repository- Live |
| These transgenic mice express the eGFPCreERT2 (Enhanced Green Fluorescent Protein and cre/ESR1) fusion gene under the direction of the mouse Tmem100, transmembrane protein 100, promoter. Transgene expression is detected in developing nephrons of the kidney, Wolfian and Mullerian ducts and vasculature of the urogenital system of hemizygous 15.5 dpc embryos. GFP fluorescence is detected in embryos 15.5dpc in age in the proximal region of the early renal vesicle, at later stages eGFP is present in the parietal epithelium adjacent to Wilms Tumor (WT1) positive cells demarcating the future podocytes of Bowman's capsule and is limited to the Tmem100 expression domain. Tamoxifen inducible Cre recombinase activity is detected in the developing kidney (nephrogenic zone), vasculature and Wolfian or Mullerian duct of mutant embryos aged 15.5dpc. Possible cre recombinase activity is detected in small renal arteries. Other possible sites of expression have not been char ..... For more information please see the full phenotype on the strain data sheet | ||
| 010576 | B6;SJL-Tg(MMTV-rtTA)4-1Jek/J | Repository- Live |
| The donating investigator claims homozygous mice are viable and fertile. These MMTV-rtTA mice have expression of the reverse tetracycline-controlled transactivator (rtTA) protein directed primarily to the breast epithelia of the mammary ductal system by the mouse mammary tumor virus (MMTV) promoter. The donating investigator also reports some rtTA expression in salivary glands (particularly in the males) as well as prostate glands. When mated to a mutant strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE, TetRE or tetO), expression of the target gene may be induced with administration of the tetracycline analog doxycycline (dox) in the double mutant offspring. These MMTV-rtTA mice are a Tet-On tool that allows conditional, dox-inducible expression of genes primarily in mammary gland epithelial cells and may be useful in studying the endocrine function of mammary tissues and/or breast cancer (for example). | ||
| 010577 | B6;SJL-Tg(tetO-Erbb2*)8-4Jek/J | Repository- Live |
| The donating investigator reports that homozygous mice are viable and fertile. These TetRE-ErbB2 (TRE-ErbB2 or TRE-Neu) transgenic mice have expression of an activated form of the rat ErbB2 regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter. When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), expression of the constitutively active ErbB2 protein may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring.
The amino acid mutation within the transmembrane domain of the ErbB2 receptor protein kinase facilitates its oligomerization and activation independent of a ligand; resulting in hyperplasia in tissues where it is expressed.
These TetRE-ErbB2 mice may be bred to generate bi-transgenic mutant mice with conditional (inducible/r ..... For more information please see the full phenotype on the strain data sheet | ||
| 008603 | C.129P2(B6)-Gt(ROSA)26Sortm1(tTA)Roos/J | Repository- Live |
| Mice heterozygous for the ROSA:LNL:tTA conditional mutation of the Gt(ROSA)26Sor locus are viable and fertile, with a loxP-flanked STOP cassette preventing transcription of a downstream optimized/modified tetracycline-controlled transactivator protein ("mtTA"). Applying both Cre-lox and Tet-Off technologies, these ROSA:LNL:tTA mutant mice may be useful to generate compound mutant mice in which expression of a tetracycline-responsive promoter element (TRE; also called tet-operator or tetO)-driven gene of interest can be both directed to the cell types defined by the chosen Cre recombinase expression, as well as turned off by the addition of tetracycline (or its analog doxycycline (dox)).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally describe ..... | ||
| 010545 | C.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ | Repository- Live |
| These L2G85.BALB/c mice harbor the CAG-luc-eGFP L2G85 transgene. Homozygous mice are viable and fertile, with widespread expression of firefly luciferase and enhanced green fluorescent protein directed by the CAG promoter (human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter). Bioluminesence is detected in heart, spleen, muscle, pancreas, skin, thymus and bone marrow. Luciferase activity is not detected in mature erythrocytes, although low levels are detected in erythrocyte precursors and varying levels of activity in all leukocyte subsets tested. Homozygotes have no reported gross physical or behavioral abnormalities. Following luciferin injection, luciferase expression is generally greater in males than females. GFP fluorescence is detected in skin (upper epidermal layers) by fluorescence microscopy. The Donating Investigator reports that for the FVB-Tg(CAG-luc,-GFP)L2G85Chco/J (STOCK#8450) strain, no GFP fluorescence ..... For more information please see the full phenotype on the strain data sheet | ||
| 013080 | C57BL/6-Actbtm3.1(Sirt1)Npa/J | Repository- Live |
| Mice that are heterozygous for the targeted mutation are viable, exhibit delayed reproduction and lower body weight. No homozygotes are produced from heterozygous crosses. Fewer than expected heterozygotes are produced from heterozygote X wildtype crosses. Overexpression of gene product (protein) is detected by Western blot analysis of white adipose tissue, brown adipose tissue, MEFs, skull calvaria cells and brain tissue. Overexpression of the protein is not detected in liver or muscle tissue. Fusion gene product (mRNA) is detected in white adipose tissue by Northern blot analysis. Beta-actin protein expression is equivalent to wildtype levels. Heterozygote knock-in mice have reduced fat mass (epididymal fat pad weight), circulating free fatty acids, leptin, adiponectin and total cholesterol. Food consumption, glucose tolerance and metabolic rate (with associated higher oxygen consumption) are increased in the mutant mice. In fasted conditions, heterozygotes have lower circul ..... For more information please see the full phenotype on the strain data sheet | ||
| 007900 | C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J | Repository- Live |
| Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.
For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation. When crossed to a strain expressing Cre recombinase in oocytes (see Stock No. 011062), this mutant mouse strain ma ..... | ||
| 009062 | C57BL/6-Magel2tm1Stw/J | Repository- Live |
| The mouse locus 7qB4/B5 (syntenic with the Prader-Willi region at chromosome position 15q11-q13 in humans) encompasses the cluster of paternally-expressed imprinted genes Magel2, Ndn, Mkrn3, and Peg12. As maternal imprinting silences the Magel2 allele, only the paternally inherited Magel2 allele is expressed. The Magel2-lacZ knock-in allele abolishes endogenous gene function and expresses a β-galactosidase fusion protein. Under control of the upstream promoter/enhancer elements, lacZ expression is directed to the same tissues as the wildtype gene. For example, β-galactosidase expression during embryogenesis is detected in central nervous system (neural tube, forebrain, midbrain and embryonic hypothalamus), peripheral nervous system (dorsal root ganglia and peripheral neurons innervating limb and trunk muscles), and some non-neuronal tissues (genital tubercle, midgut region and placenta). Adult β-galactosidase ..... For more information please see the full phenotype on the strain data sheet | ||
| 003651 | C57BL/6-Tg(Zp3-cre)93Knw/J | Repository- Live |
| This is a transgenic line in which cre expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene. This promoter normally directs expression exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain would be useful for deleting a floxed sequence specifically in the female germ line. The Donating Investigator suggests to accomplish this, females homozygous or heterozygous for the floxed allele, as well as hemizygous for the Zp3cre allele are crossed with wild type males. Progeny will carry the deleted-floxed allele. | ||
| 002356 | C57BL/6J-Tg(pPGKneobpA)3Ems/J | Repository- Live |
| Homozygous neoR transgenic mice (TgN3Ems) are viable and fertile with no apparent gross phenotype. The pPGKneobpA transgene has the mouse Pgk1 promoter directing widespread (but not necessarily uniform) expression of neoR: this renders TgN3Ems mice highly G418-resistant and results in a 5-fold increase in the approximate lethal dose of G418 (~480 mg/kg) compared to wildtype mice (~103 mg/kg). Treatment with G418 lethal dose (or higher) in transgenic mice leads to rapid onset of decreased movement, respiratory arrest, and death within minutes. Compared to wildtype mouse embryonic fibroblasts (MEFs), TgN3Ems MEFs exhibit greatly diminished G418 sensitivity: wildtype MEFs show essentially no resistance to G418 while TgN3Ems MEFs survive approximately 20-fold higher G418 levels. Importantly, TgN3Ems heterozygotes and TgN3Ems homozygotes are equally resistant to G418. These TgN3Ems transgenic mice may be useful as source of G418-resistant feeder cells for ge ..... For more information please see the full phenotype on the strain data sheet | ||
| 010543 | C;129-Hsf1tm1Ijb/J | Repository- Live |
| Mice that are heterozygous for this targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. A truncated gene product (mRNA) is detected by Northern blot analysis of embryonic cells. No gene product (protein) is detected by Western blot analysis of non-treated and heat shocked cells or brain, testis, heart and liver tissue. The prenatal lethal phenotype of homozygous mice is more severe on the 129 background than on BALB/c, C57BL/6, or ICR backgrounds. Surviving homozygotes have slowed growth with body weights approximately 78% of normal at eight weeks of age. Homozygotes exhibit abnormal chorioallantoic placenta (thinned spongiotrophoblast layer). Homozygous females are infertile due to impaired meiosis and zygotic cell division. Homozygotes are more resistant to experimentally induced skin tumors and exhibit a lower tumor burden than wild-type controls. Cultured MEFs from homozygotes are less sensitive to glucose depriv ..... For more information please see the full phenotype on the strain data sheet | ||
| 008040 | CBy.B6-Gt(ROSA)26Sortm1(HBEGF)Awai/J | Repository- Live |
| Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.
For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation. When crossed to a strain expressing Cre recombinase in the pituitary and, at lower levels, in the testes (see Stock No. > ..... | ||
| 007075 | CByJ.B6-Tg(CAG-EGFP)1Osb/J | Repository- Live |
| This transgenic mouse line with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer makes all of the tissues, with the exception of erythrocytes and hair, appear green under excitation light. Note that mice homozygous for this transgene die within the first two weeks following birth.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available. Of note, it has been the experience at The Jackson Laboratory that Stock No. 006567 (C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ) demonstrates the highest proportion of GFP expr ..... | ||
| 008450 | FVB-Tg(CAG-luc,-GFP)L2G85Chco/J | Repository- Live |
| Mice homozygous for the CAG-luc-eGFP L2G85 transgene are viable and fertile, with widespread expression of firefly luciferase and enhanced green fluorescence protein directed by the CAG promoter (human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter). Bioluminesence is detected in heart, spleen, muscle, pancreas, skin, thymus and bone marrow. Luciferase activity is not detected in mature erythrocytes, although low levels are detected in erythrocyte precursors and varying levels of activity in all leukocyte subsets tested. Homozygotes have no reported gross physical or behavioral abnormalities. Following luciferin injection, luciferase expression is generally greater in males than females. GFP fluorescence is detected in skin (upper epidermal layers) by fluorescence microscopy. The Donating Investigator reports that for the FVB-Tg(CAG-luc,-GFP)L2G85Chco/J (STOCK#8450) strain, no GFP fluorescence is detected in hematopoiet ..... For more information please see the full phenotype on the strain data sheet | ||
| 013585 | FVB-Tg(Cdh5-tTA)D5Lbjn/J | Repository- Live |
| These transgenic mice express tetracycline regulated transactivator under the direction of the mouse Cdh5, cadherin 5, promoter. When these VE-Cadherin-tTA mice are bred with mice carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE, TetRE or tetO), expression of that gene in blood vessel endothelial cells may be conditionally inactivated with administration of the tetracycline in the double mutant offspring. Tetracycline was used by the Donating Investigator in the original publication Proc Natl Acad Sci 2005;102:128-33. When crossed with a strain carrying a tetracycline-responsive promoter driven lacZ transgene, beta-galactosidase is expression is observed in VEGFR-2, CD31 expressing cells in the blood vessels of bi-transgenic day 13.5 aged embryos. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. | ||
| 006954 | FVB-Tg(Ddx4-cre)1Dcas/J | Repository- Live |
| Mice hemizygous for this Vasa-Cre transgene are viable and fertile. Transgenic cre activity is directed to male and female germ cells starting at embryonic day (e)15-e18. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequence. In such breedings, occasional hemizygous mice may exhibit variegated cre expression in skin epithelium or global cre expression (<20% incidence). Differential parent-of-origin transgene expression is observed. When the mother harbors Vasa-Cre, virtually all progeny undergo global Cre-mediated recombination, even those that do not inherit the transgene (which may be useful in converting a "floxed" allele to a null while obviating the need to perform additional crosses to remove the transgene). To achieve germ-line specific Cre-mediated recombination in offspring, paternal Vasa-Cre mice should be used.
In crosses with some floxed alleles, gl ..... | ||
| 010542 | NOD.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ | Repository- Live |
| These L2G85.NOD mice harbor the CAG-luc-eGFP L2G85 transgene. Mice homozygous for the CAG-luc-eGFP L2G85 transgene are viable and fertile, with widespread expression of firefly luciferase and enhanced green fluorescence protein directed by the CAG promoter (human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter). Bioluminesence is detected in heart, spleen, muscle, pancreas, skin, thymus and bone marrow. Luciferase activity is not detected in mature erythrocytes, although low levels are detected in erythrocyte precursors and varying levels of activity in all leukocyte subsets tested. Homozygotes have no reported gross physical or behavioral abnormalities. Following luciferin injection, luciferase expression is generally greater in males than females. GFP fluorescence is detected in skin (upper epidermal layers) by fluorescence microscopy. The Donating Investigator reports that for the FVB-Tg(CAG-luc,-GFP)L2G85Chco/J (STOC ..... For more information please see the full phenotype on the strain data sheet | ||
| 013701 | STOCK Cep290tm1Jgg/J | Repository- Live |
| These mice possess loxP sites on either side of exon 36 and 37 of the Cep290 (centrosomal protein 290) gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in studies of retinal degeneration, cilia/flagella development, and fertility. | ||
| 013731 | STOCK Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/J | Repository- Live |
| Mice homozygous for the R26R-Confetti conditional allele are viable and fertile, with a CAG promoter, loxP site, and STOP cassette preventing transcription of the downstream Brainbow 2.1 sequences. The Brainbow 2.1 region contains two loxP-flanked dimers, each uniquely positioned in head-to-tail tandem. One dimer has nuclear-localized green fluorescent protein (hrGFPII) and a reverse-oriented cytoplasmic yellow fluorescent protein (mYFP). The other dimer has cytoplasmic red fluorescent protein (tdimer2(12)) and a reverse-oriented membrane-tethered cyan fluorescent protein (mCerulean). The Brainbow2.1 region may be written as loxP-STOP-loxP-GFP-PFY-Pxol-loxP-RFP-PFC-Pxol to show the transcriptional direction of each part. When bred to mice that express Cre recombinase, the resulting offspring may have a recombination event that stochastically places one of the four fluorescent proteins into position directly downstream of the CAG promote ..... For more information please see the full phenotype on the strain data sheet | ||
| 006331 | STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J | Repository- Live |
| Homozygous "ROSA26-eGFP-DTA" mice are viable, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator reports that some homozygous males are subfertile. Mutant mice display widespread expression of EGFP, but DTA transcription is prevented by a strong transcriptional stop sequence. When bred to mice that express Cre recombinase under the control of a promoter of interest, the loxP-flanked EGFP and stop sequence are removed, and DTA expression is activated, resulting in the specific ablation of cre-expressing cells. This strain may be useful on its own as a fluorescent reporter or in combination with cre-expressing mice to produce conditional deletions of specific groups of cells. Transgenic mice also may have applications in toxicology and protein synthesis research.
For example, when crossed to a strain expressing Cre recombinase in the midbrain and dorsal spinal cord (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 008600 | STOCK Gt(ROSA)26Sortm1(tTA)Roos/J | Repository- Live |
| Mice heterozygous for the ROSA:LNL:tTA conditional mutation of the Gt(ROSA)26Sor locus are viable and fertile, with a loxP-flanked STOP cassette preventing transcription of a downstream optimized/modified tetracycline-controlled transactivator protein ("mtTA"). Applying both Cre-lox and Tet-Off technologies, these ROSA:LNL:tTA mutant mice may be useful to generate compound mutant mice in which expression of a tetracycline-responsive promoter element (TRE; also called tet-operator or tetO)-driven gene of interest can be both directed to the cell types defined by the chosen Cre recombinase expression, as well as turned off by the addition of tetracycline (or its analog doxycycline (dox)). | ||
| 012266 | STOCK Gt(ROSA)26Sortm5(ACTB-tTA)Luo/J | Repository- Live |
| Mice homozygous for this ROSA26-ZtTA (or ZtTA) conditional allele are viable and fertile, although the donating investigator reports that heterozygous mice are more healthy and fertile than homozygous mice. This ROSA26-ZtTA (or ZtTA) conditional allele is designed with a loxP-flanked β-geo transcriptional STOP cassette preventing transcription of the downstream tetracycline-controlled transactivator protein (tTA). When bred to mice that express Cre recombinase, offspring will have the STOP cassette deleted and subsequent expression of the tTA in the cre-expressing cells. The donating investigator reports that the CMV enhancer/chicken beta-actin core promoter (pCA) allows stronger and persistent expression of the downstream tTA (especially in adult cells) compared to the endogenous Gt(ROSA)26Sor locus alone. Applying both Cre-lox and Tet-Off technologies, these ZtTA mutant mice may be useful to generate compound mutant mice in which expression of a tetracyclin ..... For more information please see the full phenotype on the strain data sheet | ||
| 012871 | STOCK Pik3r1tm1Lca/J | Repository- Live |
| Mice homozygous for this p85αloxP allele are viable and fertile, with loxP sites flanking exon 7 of the targeted gene. The Pik3r1 locus encodes three proteins (p85α, p55α, and p50α) that arise from alternative transcription initiation sites; and exon 7 is the first common exon for all three isoforms. When bred to mice that express Cre recombinase, the resulting offspring will have exon 7 deleted in the cre-expressing tissue(s); splicing of upstream exons (exon 6, 1b, or 1c) directly into the downstream exon 8 results in a frameshift mutation that introduces an immediate stop codon. Such a deletion should prevent the translation of the SH2 and p110-binding domains, eliminating the ability to form a functional protein from any of the three transcription initiation sites. These mutant mice may be useful in generating conditional mutations for studying class IA phosphoinositide 3-kinases (PI3Ks) in cell growth, cell proliferation cell ..... For more information please see the full phenotype on the strain data sheet | ||
| 012620 | STOCK Trp53tm1Brd Brca1tm1Aash Tg(LGB-cre)74Acl/J | Repository- Live |
| BLG-Cre; Brca1F22-24/F22-24; p53+/- mice carry the beta-lactoglobulin (BLG)-Cre transgene, are homozygous for floxed exons 22-24 of the breast cancer 1 (Brca1) allele, and are heterozygous for p53 tumor-suppressor gene (p53) deficiency. Mice of this genotype are viable, fertile, normal in size and do not display any behavioral abnormalities. BLG-Cre; Brca1F22-24/F22-24; p53+/- females have expression of the BLG-Cre transgene during lactation; which leads to loss of Brca1 function in the mammary gland. This results in formation of mammary tumors exhibiting high grade central necrosis and metaplastic elements in the form of spindle cell and squamous cell differentiation; as seen in human basal-like breast cancers and BRCA1 mutation carriers. Heterozygosity for the mutant p53 allele accelerates the formation of mammary tumors. This strain may be useful for studying human basal-like cancer and breast ..... For more information please see the full phenotype on the strain data sheet | ||
| 013749 | STOCK Tg(ACTB-EGFP,-tdTomato)11Luo/J | Repository- Live |
| Homozygous MADM-11GT mice are viable and fertile with no gross behavioral or observable abnormalities. The MADM-11GT allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP), a beta-globin intronic sequence containing an frt site and a loxP-flanked neomycin resistance gene, and the MYC-tagged C-terminal portion of a red fluorescent protein (tdTomato) all inserted into the Hipp11 locus on chromosome 11 (cytoband A1 at ~3cM between the Eif4enif1 and Drg1 loci). These MADM-11GT mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to MADM-11TG mice harboring a reciprocal mutation at the same locus (see Stock No. 013751). The resulting GT/TG offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygou ..... For more information please see the full phenotype on the strain data sheet | ||
| 014092 | STOCK Tg(ACTB-tTA2,-MAPT/lacZ)1Luo/J | Repository- Live |
| Homozygous CAG-stop-tTA2 transgenic mice are viable and fertile. CAG-stop-tTA2 transgenic mice harbor the ii-CAG-stop-tTA2-IRES-tau-lacZ-ii transgene; designed with a loxP-flanked transcriptional STOP cassette preventing transcription of the downstream modified tetracycline-regulated transactivator (tTA2). The ii-CAG-stop-tTA2-IRES-tau-lacZ-ii transgene is flanked by two copies of the chicken β-globin HS4 insulator on each side to preserve expression fidelity (see additional information below). When bred to mice that express a tamoxifen-inducible Cre recombinase (CreERT2), administration of tamoxifen to the double mutant offspring allows the CreERT2 fusion protein to enter the nucleus of the cre-expressing cells; this deletes the STOP cassette and results in expression of tTA2. The donating investigator reports that tau-lacZ fusion protein expression in the tamoxifen-treated double mutant offspring is faint. Of note, the donating investigator h ..... For more information please see the full phenotype on the strain data sheet | ||
| 013751 | STOCK Tg(ACTB-tdTomato,-EGFP)11Luo/J | Repository- Live |
| Homozygous MADM-11TG mice are viable and fertile with no gross behavioral or observable abnormalities. The MADM-11TG allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of a red fluorescent protein (tdTomato), a beta-globin intronic sequence containing an frt site and a loxP-flanked neomycin resistance gene, and the C-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP) all inserted into the Hipp11 locus on chromosome 11 (cytoband A1 at ~3cM between the Eif4enif1 and Drg1 loci). These MADM-11TG mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to MADM-11GT mice harboring a reciprocal mutation at the same locus (see Stock No. 013749). The resulting TG/GT offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous"), and mu ..... For more information please see the full phenotype on the strain data sheet | ||
| 003116 | STOCK Tg(CAG-EGFP)D4Nagy/J | Repository- Live |
| This transgenic strain carries the Enhanced Green Fluorescent Protein (Clontech) driven by chicken beta-actin promoter and CMV intermediate early enhancer. The transgene was found to have integrated on the X chromosome. Mice of this strain, and cells derived from them, can be distinguished from wildtype on the basis of fluorescence. Transgene expression is seen as early as ~embryonic day 2.75, as morula stage embryos begin to compact. Expression of EGFP is spatiotemporally widespread, though levels differ between different lineages (e.g., expression is slightly higher in the heart). As the transgene is located on the X chromosome, transgenic males will exclusively transmit the EGFP transgene to their female offspring, providing the basis for a non-invasive sexing assay based on green fluorescence. These mice may be useful for sex discrimination prior to overt sexual dimorphism (embryonic day 12.5), monitoring X-inactivation, and may be a source of fluorescently marked cells/tiss ..... For more information please see the full phenotype on the strain data sheet | ||
| 003208 | STOCK Tg(DR4)1Jae/J | Repository- Live |
| MEFs prepared from the DR-4 mouse strain displayed resistance to concentrations of the drugs G418, 6-thioguanine, puromycin and hygromycin well above those used normally for the selection of drug-resistant ES cells. This mouse strain represents an economical donor for the production of multiple-drug resistant mouse embryonic fibroblasts (MEFs). | ||
| 011062 | STOCK Tg(Gdf9-cre)5092Coo/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse growth differentiation factor 9 (Gdf9) promoter. Cre recombinase expression is detected in oocytes of the primordial follicles by postnatal day 3 and in oocytes, but not somatic cells, of all follicles at the primary, secondary and later stages by 24 days. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in the offspring. This mutant mouse strain may be useful in studies of studies of folliculogenesis and oocyte development. | ||
| 008208 | STOCK Tg(Stra8-cre)1Reb/J | Repository- Live |
| Homozygous Stra8-cre transgenic mice are viable and fertile, with expression of an optimized variant of Cre recombinase (iCre) directed by the 1.4 kb mouse Stra8 (stimulated by retinoic acid gene 8) genomic promoter fragment. Postnatal Cre-recombinase expression is first observed in testes at postnatal day (P)3 (early-stage spermatogonia), increases out to P7 (pre-leptotene-stage spermatocytes), and is not detected in other tissues examined (including ovaries). In addition, no cre expression is reported in male or female embryos. When Stra8-cre transgenic males are bred with female mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence, specifically during these stages of spermatogenesis. These Stra8-cre transgenic mice may be useful in generating conditional knockouts in postnatal, premeiotic, male germ cells for studying spermatogenesis. | ||
| 013752 | STOCK Tg(TCF/Lef1-HIST1H2BB/EGFP)61Hadj/J | Repository- Live |
| TCF/Lef:H2B-GFP transgenic mice express an H2B-EGFP fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Green Fluorescent Protein gene) under the control of six copies of a T cell specific transcription factor/lymphoid enhancer-binding factor 1 (TCF/Lef1) response element and a heat shock protein 1B (Hspa1b) minimal promoter. Mice homozygous for the transgene are viable, fertile, and normal in size. Wnt (wingless-related MMTV) family members are required for triggering embryonic axis formation and for proper development. Wnt signaling results in phosphorylation and nuclear localization of β-catenin, a transcriptional co-activator protein, which, together with the TCF/Lef family of transcription factors, induces the transcription of downstream genes. TCF/Lef:H2B-GFP transgenic mice contain 6 copies of nuclear localized TCF/Lef1 DNA binding sites, which provides increased sensitivity to Wnt/&be ..... For more information please see the full phenotype on the strain data sheet | ||
| 012345 | STOCK Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo/J | Repository- Live |
| Hemizygous TRE-Bi-SG-T line 1.1 transgenic mice are viable and fertile, with no reported phenotypic abnormalities. The TRE-Bi-SG-T transgene has Myc-tagged tdTomato and full-length mouse synaptophysin/mut4EGFP fusion protein (Syp-GFP) expression under the control of the bi-directional tet-responsive promoter (tetO or TRE). When bred with another mouse expressing tetracycline-controlled transactivator protein (tTA) or reverse tetracycline-controlled transactivator protein (rtTA), tdTomato and Syp-GFP fusion protein expression can be regulated with the tetracycline analog doxycycline (dox) in the resulting double mutant offspring. In tTA(-dox) or rtTA(+dox)-expressing cells, tdTomato expression is directed to the entire cell, while GFP expression is directed to the synapse/synaptic vesicle. The donating investigator reports that direct GFP fluorescence and direct tdTomato fluorescence may be visualized in these mice when tTA is present/dox is absent. In addition, the three Myc epi ..... For more information please see the full phenotype on the strain data sheet | ||
| 013115 | B6.Cg-Rag1tm1Mom Tg(UBC-GFP)30Scha/J | Research Strain |
| Tg(UBC-GFP)30Scha generates green fluorescent protein (GFP) expression in all cells of the body with cell lineage-specific variation in expression level. Hematopoetic cells have high expression levels compared with other cells and T cells have a 2-fold higher GFP expression level than that in CD19+B220+ B cells. The Rag1tm1Mom targeted disruption, which blocks the intragenic recombination essential for T and B cell antigen receptor formation, leaves homozygous mice immunocompromised due to the inability to form functional, mature T and B cells. This strain, which combines this targeted mutation with this transgene, is a universal host that does not reject engraftment regardless of histoincompatibility and permits detection of host-derived embryos or tissues by detection of GFP, as long as the donor tissue is not also engineered to express GFP. Animals from this strain can also be used as sentinel mice in specific pathogen free environments. ..... For more information please see the full phenotype on the strain data sheet | ||
| 002484 | 129-Alpltm1Sor/J | Cryopreserved - Ready for recovery |
| Mice heterozygous for the Alpltm1Sor targeted mutation appear normal and viable. Homozygous mutant mice are perinatal lethals but can be rescued by pyridoxal treatment. Surviving homozygotes develop epilepsy due to reduced GABA levels in the brain. Bone formation does not appear to be grossly affected in untreated animals, but treated animals exhibit cranial dysmorphology. The targeting vector contained both a b-galalactosidase and a neomycin genes (beta-geo), both of which are under the control of the Alpl promoter and are thus expressed in a tissue specific manner. Specifically, expression occurs in developing bones and in primordial germ cells (PGC), and the beta-galactosidase thus serves as a marker for these tissues. The marker for PGC's is particularly significant because the current marker (alkaline phosphatase staining) is only useful to study early germ cell migration. | ||
| 008201 | B6.129-Sepp1tm1Rfb/J | Cryopreserved - Ready for recovery |
| Mice heterozygous for this targeted mutation are viable and fertile. No RNA or selenoprotein P (Se-P) protein expression from the targeted gene is observed in plasma. Homozygous (Sepp1-deficient) mice are viable with altered selenium metabolism rendering them intolerant of low dietary selenium intake and resulting in significantly shortened life span. Homozygotes have lower brain selenium concentrations and develop progressive neurological dysfunction (impaired movement and coordination); the progression of which is preventable (but not reversible) with dietary selenium supplement. Homozygous females are fertile but have difficulty producing and raising pups. Homozygous males have sharply reduced fertility due to flagellar structural defects ("kinked sperm") which, unlike the neurological phenotype, are not prevented with dietary selenium supplement. Sepp1-deficient mice, supplemented with dietary selenium and infected with an African Trypanosomiasis parasite, exhibit increased ..... For more information please see the full phenotype on the strain data sheet | ||
| 009602 | B6.129S4(Cg)-Kcnn2tm2Jpad/J | Cryopreserved - Ready for recovery |
| The SK2T mutant allele has a tetracycline-based genetic switch inserted into the 5' UTR of the targeted gene, just upstream of the translation initiation site. This genetic switch harbors both the tetracycline-controlled transactivator protein (tTA) as well as the tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator). The donating investigator reports that long term administration of tetracycline (or its analog doxycycline (dox)) does not block transcription of the downstream Kcnn2 locus. The donating investigator also reports that homozygous mice are viable but do not thrive without dox treatment. SK2-tTA heterozygotes (SK2T) exhibit approximately ten-fold overexpression of SK2 protein before dox treatment. In the absence of dox, homozygous mice exhibit enhanced SK channel-mediated restriction of glutamatergic activity in CA1 neurons, attenuated hippocampal synaptic plasticity, impaired spatial learning ..... For more information please see the full phenotype on the strain data sheet | ||
| 009603 | B6.129S4-Kcnn3tm1Jpad/J | Cryopreserved - Ready for recovery |
| The SK3T mutant allele has a tetracycline-based genetic switch inserted into the 5' UTR of the targeted gene, just upstream of the translation initiation site. This genetic switch harbors both the tetracycline-controlled transactivator protein (tTA) as well as the tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator); allowing transcription of the downstream Kcnn3 locus to be blocked by administration of tetracycline (or its analog doxycycline (dox)). SK3-tTA homozygotes (SK3T/T) exhibit approximately three-fold overexpression of SK3 before dox treatment, and SK3 expression is effectively eliminated by addition of dox. Heterozygous mice exhibit similar expression from both their wild-type and SK3T mutant allele before dox treatment, and no SK3 expression from the mutant allele during dox treatment. In the absence of dox, homozygous mice exhibit abnormal respiratory responses to hypoxia. Homozygous ..... For more information please see the full phenotype on the strain data sheet | ||
| 005897 | B6.129S4-Ppardtm1Rev/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 4 of the targeted gene is deleted in the tissue of interest, leading to premature termination of the translation product upstream of the DNA binding domain. This strain may be useful in generating tissue-specific mutants of the floxed allele for use in studies including embryo development, adipocyte physiology, fat metabolism and storage, inflammation, and cancer. | ||
| 005901 | B6.129S4-Ppardtm2Rev/J | Cryopreserved - Ready for recovery |
| Heterozygous mice are viable and fertile. All homozygous mice die in utero. Macrophages homozygous for this mutation have no transcriptional response to very low-density lipoprotein treatment. No evaluation of lacZ expression is published. The donating investigator reports homozygous mice on this background have a similar, albeit earlier, embryonic phenotype as the exon 4 deleted mutants described in other publications (Barak PNAS 2002 99:303-8, Chawla PNAS 2003 100:1268-73, and Lee Science 2003 302:453-7). Heterozygous mice or homozygous embryo-derived cells may be useful in studies of embryo development, adipocyte physiology, fat metabolism and storage, inflammation, and cancer. | ||
| 003823 | B6.129S4-Ttpatm1Far/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous null for the Ttpa gene are viable, normal in size and do not display any gross physical abnormalities. The Ttpa protein product is required for the maintenance of proper alpha-tocopherol levels, the major form of vitamin E in plasma and tissues. The absence of Ttpa protein product in homozygous-null animals results in a corresponding 95% reduction in alpha-tocopherol. Low levels of alpha-tocopherol render female mice infertile, a condition that can be addressed with vitamin E supplements. Male fertility is unimpaired. These mice provide a viable model for studying vitamin E deficiency. | ||
| 004267 | B6.129S6-Dnmt3ltm1Bes/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for this targeted allele are viable, normal in size, do not display any gross physical or behavioral abnormalities, but are sterile. Adult homozygous males display severe hypogonadism, Sertoli-cell-only phenotype and apoptotic death of spermatocytes prior to pachytene with loss of spermatogonia progressing to nonsyndromic azoospermia in later adulthood. Extreme abnormalities of meitotic pairing occur. There is a failure to methylate transposons in prospermatogonia with expression of very high levels of both LTR and non-LTR transposons in spermatogonia and spermatocytes. Homozygous females have normal oogenesis. Heterozygous progeny of homozygous females do not develop past 9.5 day post coitum, with pericardial edema with exencephaly and other neural tube defects. Maternally imprinted gene sequence that is usually heavily methylated in control oocytes is undermethylated in mutant mice oocytes. Paternally imprinted gene sequence is not effected and global genome ..... For more information please see the full phenotype on the strain data sheet | ||
| 002741 | B6.129S7-Alpltm1Sor/J | Cryopreserved - Ready for recovery |
| Mice heterozygous for the Alpltm1Sor targeted mutation appear normal and viable. Homozygous mutant mice are perinatal lethals but can be rescued by pyridoxal treatment. Surviving homozygotes develop epilepsy due to reduced GABA levels in the brain. Bone formation does not appear to be grossly affected in untreated animals, but treated animals exhibit cranial dysmorphology. The targeting vector contained both a b-galalactosidase and a neomycin genes (beta-geo), both of which are under the control of the Alpl promoter and are thus expressed in a tissue specific manner. Specifically, expression occurs in developing bones and in primordial germ cells (PGC), and the beta-galactosidase thus serves as a marker for these tissues. The marker for PGC's is particularly significant because the current marker (alkaline phosphatase staining) is only useful to study early germ cell migration. | ||
| 006262 | B6.129X1-Fut2tm1Sdo/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice have a chinchilla (gray) coat color, while heterozygotes have the typical black coat color expected for the C57BL/6J genetic background. Alpha (1,2) fucosylated glycans are not detected in the uterine epithelia from homozygotes at estrus. Beta galactosidase staining is detected in endocervial and uterine gland mucus secreting cells, stomach foveolar pit cells and chief cells, and colon goblet cells. The pattern of beta galactosidase activity mimics the endogenous expression pattern of the endogenous gene. This mutant mouse strain represents a model of the nonsecretor ABH histo-blood group antigen, which confers resistance to Norwalk virus infection, and may be useful in studies of reproductive biology, gastrointestinal tract epithelium, and the function of fucosylated glycans.
This strain was transferred fr ..... For more information please see the full phenotype on the strain data sheet | ||
| 009642 | B6.Cg(129)-Tg(Gh1-cre)1Sac/J | Cryopreserved - Ready for recovery |
| These transgenic mice express Cre recombinase under the control of the human Growth Hormone (GH1) Locus Control Region and the rat growth hormone 1, (Gh1) promoter. When crossed with a reporter strain, Cre recombinase expression is seen during development and in the adult in pituitary somatotropes. Weaker expression is detected in lactotropes. Cre expression is detected in the forelimb skin of embryos aged embryonic day 16.5-17.5, and weaker expression in adult kidney, ovary, and testis. Southern blot analysis reveals approximately 50 copies or the transgene segregated to one locus. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study developmentally critical gene function in the anterior pi ..... For more information please see the full phenotype on the strain data sheet | ||
| 008608 | B6.Cg-Dmc1tm1Jcs/JcsJ | Cryopreserved - Ready for recovery |
| While mice heterozygous for this meiosis-specific RecA homolog (Dmc1) mutation are viable and fertile, homozygotes are viable but sterile due to arrest of gametogenesis in the first meiotic prophase (prophase I). No RNA message from the targeted gene is observed in homozygous testis. Homozygous males have reduced testis size and no mature spermatozoa in the epididymis; spermatocytes undergo meiotic arrest from defective double strand break repair and extensive chromosome asynapsis. Homozygous females exhibit defective oogenesis due to similar aberrations occurring at the pachytene stage or earlier and thus have small, malformed ovaries with complete depletion of oocytes and follicles by adulthood. These Dmc1-mutant mice may be useful in studying gametogenesis and meiosis (including double strand breaks, chromosome asynapsis, DNA repair mechanisms, homologous recombination, and the pachytene checkpoint). | ||
| 007920 | B6.Cg-Gt(ROSA)26Sortm2(CAG-EYFP)Hze/J | Cryopreserved - Ready for recovery |
| Ai2 mice hemizygous for this Rosa-CAG-LSL-EYFP conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream enhanced yellow fluorescent protein (EYFP). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of EYFP. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, EYFP expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai2 mice do not express EYFP prior to introduction of Cre recombinase and EYFP fluorescence following exposure to cre is weak but easily detected by mRNA (in situ hybridization) and antibody staining (immunohistochemistry). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-mediated ..... For more information please see the full phenotype on the strain data sheet | ||
| 006407 | B6.Cg-Gusbmps/BrkJ | Cryopreserved - Ready for recovery |
| Mice homozygous for the "mps" (mucopolysaccharidosis type VII or MPS VII) mutation are devoid of expression of the lysosomal enzyme beta glucuronidase. Homozygous animals are viable, but females have a deficiency in lactation. Skeletal and connective tissue anomalies in both males and females are believed to prevent successful breeding. As this mutation is recessive, heterozygous mice are phenotypically similar to wildtype. Homozygotes exhibit short and thickened long bones (smaller than heterozygous or wildtype littermates), "pug type" appearance of the nose, hepatomegaly, splenomegaly, corneal clouding, and deafness. These mice have the H2b haplotype typical of inbred C57BL/6 mice. MPS VII mice are a model of the beta glucuronidase enzyme deficiency in humans called Sly Disease. They may be useful in developing new therapies (enzyme replacement, cell transplantation, gene therapy) broadly applicable to other lysosomal storage diseases. | ||
| 002283 | B6.Cg-KitW-19H/EiJ | Cryopreserved - Ready for recovery |
| This deletion is a dominant mutation causing white spotting on the feet, tail, belly, and occasionally elsewhere. Homozygosity is embryonic lethal. On the C57BL/6 background approximately 60% of heterozygous females have a closed vagina. The ovaries from these heterozygotes are normal and fine for transplantation. On the C57BL/6 background in the presence of the YAKR/J Chromosome (available from Stock No. 007250), KitW-19H heterozygosity results in sex reversed XY females. | ||
| 009643 | B6.Cg-Tg(Lhb-cre)1Sac/J | Cryopreserved - Ready for recovery |
| These transgenic mice express Cre recombinase under the control of the bovine LHB, luteinizing hormone beta polypeptide promoter. Although the transgenic construct contains sequence encoding a fusion gene of EGFP and Cre recombinase, no EGFP fluorescence is detected. When crossed with a reporter strain, Cre recombinase expression is seen in mature pituitary gonadotropes, specifically in cells that produce luteinizing hormone beta. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study developmentally critical gene function in the pituitary. | ||
| 008735 | B6.Cg-Tg(Wap-cre)11738Mam/JKnwJ | Cryopreserved - Ready for recovery |
| WAP-Cre transgenic mice are viable and fertile, with expression of P1 Cre recombinase under the control of the mouse Wap (whey acidic protein) promoter. When bred with other mutant mice containing loxP-flanked sequence(s), Cre-mediated recombination will result in deletion of the floxed sequence(s) in mammary gland epithelium. Deletion of floxed sequences is reported in brain tissue from WAP-Cre mice on a mixed genetic background but has not been examined in this strain. Maximum Cre recombinase activity is achieved during pregnancy and lactation, but in virgin mice small numbers of mammary epithelial cells show evidence of estrus cycle-related Cre recombinase activity. These WAP-Cre transgenic mice may be useful in generating conditional mutations in mammary glands for studying mammary physiology and processes such as breast development and breast cancer.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently ..... | ||
| 009344 | B6.Cg-Tg(tetO-Ifng)184Pop/J | Cryopreserved - Ready for recovery |
| The donating investigator reports that homozygous mice are viable and fertile. These TRE/IFN-γ transgenic mice have expression of mouse interferon-gamma (IFN-γ) regulated by the tetracycline-responsive promoter (tetO [also called tetracycline-responsive element (TRE or tet-operator)] fused to the human cytomegalovirus minimal promoter). When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), expression of IFN-γ may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring. Because interferon-gamma (IFN-γ) is a pleiotropic cytokine secreted by activated T-lymphocytes and natural killer cells, these line 184 TRE/IFN-γ mice may be bred to generate bi-transgenic mutant mice with conditional (inducible/reversible) expression of IFN-γ for studying antiviral responses, immune surveillance, inhibiting cellular prol ..... For more information please see the full phenotype on the strain data sheet | ||
| 003394 | B6.FVB-Tg(Zp3-cre)3Mrt/J | Cryopreserved - Ready for recovery |
| This is a transgenic line in which cre expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene. This promoter normally directs expression exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain would be useful for deleting a floxed sequence specifically in the female germ line. Homozygotes females produce small litters (3.7 pups/litter; Genesis 26:110-112, 2000). An alternative transgenic strain that bears a similar transgenic construct and produces larger litters (~6 pups/litter) is available: C57BL/6-Tg(Zp3-cre)93Knw/J (Stock No. 003651). | ||
| 004521 | B6.PL-Nppclbab/GrsrJ | Cryopreserved - Ready for recovery |
| 003552 | B6129-Tg(Wap-cre)11738Mam/J | Cryopreserved - Ready for recovery |
| This transgenic strain expresses P1 Cre recombinase under the control of the Wap (whey acidic protein) promoter. In mammary gland tissues, the Wap promoter directs expression to secretory epithelium. A maximum of Cre mediated recombination is achieved during pregnancy and lactation, but recombined cells are still present after involution and complete remodeling of the gland. Cre recombinase expression is not entirely restricted to mammary gland, however. A limited amount of Cre activity has been reported in brain tissue. | ||
| 008678 | B6;129-Ubbtm1Rrk/J | Cryopreserved - Ready for recovery |
| Mice heterozygous for the targeted allele are viable and fertile. This polyubiquitin B (Ubb) mutation is characterized by a GFP-puror fusion protein "knock-in" allele that also abolishes endogenous gene function. Direct visualization of GFP fluorescence is observed in ovaries, testes, hypothalamus (arcuate nucleus) and cerebral cortex. Homozygotes have no Ubb mRNA observed in the various tissues tested, and are viable but sterile due to failure of germ cells to progress through meiotic prophase I and hypogonadism. Homozygotes also exhibit a complex metabolic phenotype initially characterized by dysfunction of neurons within the central nervous system accompanied by retarded perinatal growth that progresses to adult-onset obesity linked to selective hypothalamic neurodegeneration. Homozygotes also develop adult-onset hyperleptinemia (but normal levels of circulating glucose and insulin) as a consequence of increased fat content. These Ubb-mutant mice may be useful in studyin ..... For more information please see the full phenotype on the strain data sheet | ||
| 010988 | B6;129P-Cyp11a1tm1(GFP/cre)Pzg/J | Cryopreserved - Ready for recovery |
| A GFP-Cre cassette (GC) was inserted into the Cyp11a1, cytochrome P450, family 11, subfamily a, polypeptide 1, locus. Cre activity is detected in male testes, male and female adrenal glands and some kidneys from heterozygous E15.5 embryos. No green fluorescence was detected by direct fluorescence microscopy, however, immunohistochemical analysis revealed GFP expression. Transgene expression mimics the endogenous expression pattern. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous. This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 010984 | B6;129P-Upk1btm1Pzg/J | Cryopreserved - Ready for recovery |
| These mutant mice express Red Fluorescent Protein from the endogenous Upk1b (uroplakin 1B) locus. Red fluorescence is detected in the bladder and gonads of the urogenital system from E15.5 heterozygous embryos. Fluorescence mimics the endogenous expression pattern. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous.
This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 008042 | B6;129P2-Adam2tm1Dgm/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this fertilin β (Adam2) mutant allele are viable with no gross phenotypic abnormalities reported. Both precursor and processed fertilin β proteins were absent from spermatogenic cells and mature sperm isolated from homozygous males. While homozygous females are fertile with normal egg activation, homozygous males exhibit sperm deficiencies in sperm-egg membrane adhesion, sperm-egg fusion, migration from the uterus into the oviduct, and binding to the egg zona pellucida, rendering them infertile. These fertilin β (Adam2) mutant mice may be useful in reproductive biology studies; specifically to determine the role of ADAM (A Disintegrin And Metalloprotease) family proteins in sperm-egg interactions, fertilization, and spermatogenesis. These mice may also be useful in conjunction with other ADAM family mutant mice, including the cyritestin (Adam3)-deficient strain (see Stock No. 00 ..... For more information please see the full phenotype on the strain data sheet | ||
| 008043 | B6;129P2-Adam3tm1Pmkf/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this cyritestin (Adam3) mutant allele are viable with no gross phenotypic abnormalities reported. No protein product is detected from the targeted gene in sperm isolated from homozygous males. While homozygous females are fertile, homozygous males exhibit sperm deficiencies in adhesion to the egg zona pellucida and to the egg plasma membrane rendering them infertile. These cyritestin (Adam3) mutant mice may be useful in reproductive biology studies; specifically to determine the role of ADAM (A Disintegrin And Metalloprotease) family proteins in sperm-egg interactions, fertilization, and spermatogenesis. These mice may also be useful in conjunction with other ADAM family mutant mice, including the fertilin β (Adam2)-deficient strain (see Stock No. 008042). | ||
| 013139 | B6;129P2-Ifitm3tm1(RFP)Pzg/J | Cryopreserved - Ready for recovery |
| Red fluorescence is detected in ovaries from heterozygous female embryos (E15.5) and testis tissue of male heterozygous embryos. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous.
This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 010719 | B6;129P2-Prdm9tm1Ymat/J | Cryopreserved - Ready for recovery |
| Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are viable but sterile. No gene product (mRNA or protein) is detected by RT-PCR analysis of testes from homozygotes. Male homozygotes display decreased testis weight and incomplete spermatogenesis (arrest at the pachytene stage). Female homozygotes exhibit reduced germ cell number in neonatal ovaries and no forming follicles. Homozygotes have abnormal homologous chromosome pairing, impaired double strand break repair and defective sex body (XY body) formation. This mutant mouse strain may be useful in studies of meiotic arrest, infertility, and double-stranded break repair. | ||
| 004153 | B6;129S-Mtap7Gt(ROSABetageo)1Sor/J | Cryopreserved - Ready for recovery |
| At birth, mice homozygous for the gene-trapped Mtap7 allele are viable, normal in size and do not display any gross physical or behavioral abnormalities. Although trace amounts of a presumably nonfunctional transcript can be detected in testis tissue, no protein product is immunodetectable. Male homozygotes are sterile. Expression of the reporter gene (B-galactosidase from the Bgeo fusion gene) employed by the gene trap vector indicates that the Mtap7 promoter directs expression in various tissues with highest levels seen in the seminiferous tubules. During the first wave of spermatogenesis at 5 weeks of age, deformed spermatids can be observed. Abnormalities are attributed to aberrant microtubule organization. Microtubule aberrations are also observed in Sertoli cells. Gradual loss of germ cells occurs. At three months of age, the testes of homozygous mutants are less than one-third the size of those of heterozygous littermates. | ||
| 010986 | B6;129S-Osr2tm1Pzg/J | Cryopreserved - Ready for recovery |
| These Osr2-RFP mutant mice express Red Fluorescent Protein from the endogenous Osr2, odd-skipped related 2 (Drosophila), locus. Strong red fluorescence is detected the head, testis and male and female genital ducts and kidneys from heterozygous E15.5 embryos. Fluorescence mimics the endogenous expression pattern. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are not viable. The Donating Investigator reports that homozygotes die just after birth, most likely due to a cleft palate. This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP). | ||
| 007032 | B6;129S-Wnt4tm1.1Bhr/BhrEiJ | Cryopreserved - Ready for recovery |
| This strain contains loxP sites flanking exon 2 of Wnt4 resulting in a Cre-dependent conditional null allele. Homozygotes are normal. Studies by Kobayashi et al., determined that when this conditional allele is exposed to Cre expression by Amhr2tm3(cre)Bhr Mullerian duct regression proceeds normally. | ||
| 002317 | B6;129S7-Alpltm1Sor/J | Cryopreserved - Ready for recovery |
| Mice heterozygous for the Alpltm1Sor targeted mutation appear normal and viable. Homozygous mutant mice are perinatal lethals but can be rescued by pyridoxal treatment. Surviving homozygotes develop epilepsy due to reduced GABA levels in the brain. Bone formation does not appear to be grossly affected in untreated animals, but treated animals exhibit cranial dysmorphology. The targeting vector contained both a b-galalactosidase and a neomycin genes (beta-geo), both of which are under the control of the Alpl promoter and are thus expressed in a tissue specific manner. Specifically, expression occurs in developing bones and in primordial germ cells (PGC), and the beta-galactosidase thus serves as a marker for these tissues. The marker for PGC's is particularly significant because the current marker (alkaline phosphatase staining) is only useful to study early germ cell migration. | ||
| 011129 | B6;129X1-Cdc25btm1Hpw/J | Cryopreserved - Ready for recovery |
| These mice carry a floxed allele of Cdc25b (cell division cycle 25 homolog B (S. pombe)). When bred to mice with a cre recombinase gene, exons 2-14 of the targeted gene are deleted in the cre expressing tissues of the offspring. This strain may be useful in studies of cell division and oocyte maturation. | ||
| 002709 | B6;C3-Tg(TettTALuc)1Dgs/J | Cryopreserved - Ready for recovery |
| Transgenic mice were produced by co-microinjection of modified tetracycline-controlled transactivator (tTA) and luciferase genes placed under the control of tetracycline-responsive promoter elements (TREs). Expression of tTA in transgenic mice is both inducible and autoregulatory; expression of the transgenes are suppressed in the presence of tetracycline and highly expressed in its absence. Induced transgene expression levels are higher than those achieved in strains utilizing a constitutive tTA system. Luciferase expression (in the absence of tetracycline) has been demonstrated in all organs examined - spleen, thymus, lung, liver, kidney, heart, cerebrum, cerebellum, lymph nodes, testes. High activity is found in thymus and lung; low expression is found in liver and kidney. Induction ranges from 2-fold in testes to 150-fold in thymus. The founder line of this strain is #17, as reported in the primary reference. Homozygous mice are viable and fertile. | ||
| 004966 | B6;CBA-Tg(Acrv1-EGFP)2727Redd/J | Cryopreserved - Ready for recovery |
| Mice carrying this "-408SP10-gfp" transgene express Enhanced Green Fluorescent Protein (EGFP) under the direction of the -408 to +28 bp mouse SP-10 (Acrv1) promoter region that contains the necessary transcription regulation signals responsible for both temporal and testis-specific gene expression in transgenic mice. In hemizygous mice, EGFP fluorescence is detected in postmeiotic round spermatid cells of testis beginning from post natal day 21 through adult life. Transgene expression is detected in testis by by northern hybridization as well as RT-PCR analysis. In situ hybridization examination of testis from transgenic mice reveals EGFP is expressed in a pattern mimicking endogenous Acrv1 spatial and temporal expression. This strain may be useful in studies related to spermatogenesis. | ||
| 004200 | B6;CBACa Aw-J/A-Npr2cn-2J/GrsrJ | Cryopreserved - Ready for recovery |
| Mice carrying the Npr2cn-2J display mutation short thick femurs, round heads, disorganized growth plates, and relatively normal vertebrae; A one month old female exhibited the same phenotype as above but also had splayed ribs. | ||
| 003466 | B6;D2-Tg(Sycp1-cre)4Min/J | Cryopreserved - Ready for recovery |
| 010574 | B6;SJL-Tg(Gh1-rtTA)4-3Jek/J | Cryopreserved - Ready for recovery |
| The donating investigator reports that homozygous mice are viable and fertile. These GH-rtTA transgenic mice have expression of the reverse tetracycline-controlled transactivator (rtTA) protein directed to GH1-producing cells (pituitary somatotropes) by the rat growth hormone (Gh1) promoter. When mated to a mutant strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE, TetRE or tetO), expression of the target gene in GH1-producing cells may be induced with administration of the tetracycline analog doxycycline (dox) in the double mutant offspring. These GH-rtTA transgenic mice are a Tet-On tool that allows conditional, dox-inducible expression of genes in GH1-producing cells/tissues (such as pituitary somatotropes) and may be useful for studying pituitary hormone secretion and proliferation. | ||
| 010573 | B6;SJL-Tg(Prl-tTA)6-5Jek/J | Cryopreserved - Ready for recovery |
| The donating investigator claims homozygous mice are viable and fertile. These Prl-tTA transgenic mice have expression of the tetracycline-controlled transactivator (tTA) protein directed to PRL-producing cells (pituitary lactotropes/mammotropes) by the rat prolactin (Prl) promoter. The donating investigator also reports some expression in testis. When mated to a mutant strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE, TetRE or tetO), expression of the target gene in the PRL-producing cells may be conditionally abolished with administration of the tetracycline analog doxycycline (dox) in the double mutant offspring. These Prl-tTA transgenic mice are a Tet-Off tool that allows dox-conditional expression of genes in PRL-producing cells/tissues (such as pituitary lactotropes/mammotropes) and may be useful for studying pituitary hormone secretion and proliferation. | ||
| 002369 | B6;SJL-Tg(c177-lacZ)226Bri/J | Cryopreserved - Ready for recovery |
| Transgenic mice show a strong expression of the lacZ gene in round spermatids and subsequent stages of spermatogenesis. These mice can be used as a source of germ cells and transplanted into busulfan treated mice (busulfan (40 mg/kg i.p.) destroys spermatogenic stem cells). LacZ serves as a reporter for the transplanted cells. Following transplantation, spermatogonia with stem cell potential establish themselves on the basal membrane of the recipient seminiferous tubule and begin to replicate. This strain has a higher copy number of the transgene than the TgN(c177lacZ)227Bri strain and may be distinguished from it by a dot blot. Cells from these two lines may therefore be mixed prior to injection and be distinguished from each other in the progeny. | ||
| 002372 | B6;SJL-Tg(c177-lacZ)227Bri/J | Cryopreserved - Ready for recovery |
| B6SJLF1/J mice (Stock No. 100012) may be used as controls. These only provide an approximate genetic match to this B6,SJL background. | ||
| 010575 | B6;SJL-Tg(tetO-Egfr*)2-9Jek/J | Cryopreserved - Ready for recovery |
| The donating investigator reports that homozygous mice are viable and fertile. These TetRE-EGFR-tr (TRE-EGFR-tr) transgenic mice express a dominant negative, cytoplasmic tyrosine kinase domain-deleted mutant form of epidermal growth factor receptor (EGFR-tr) regulated by the tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator) and cytomegalovirus minimal promoter. When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue EGFR-tr expression may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring. EGFR-tr expression disrupts subsequent activation of signal transduction pathways that are involved in regulating cellular proliferation, differentiation, and survival. These TetRE-EGFR-tr mice may be bred to generate bi-transgenic mutant mice with conditional (inducible/reversible) expressi ..... For more information please see the full phenotype on the strain data sheet | ||
| 003299 | B6;SWJ-Tg(TIMP3-lacZ)7Jeb/J | Cryopreserved - Ready for recovery |
| These transgenic mice show no phenotypic defects. LacZ expression is observed in the eye, specifically in the corneal endothelium and ganglion cell layer. LacZ expression is also observed in the kidney, choroid plexus, testes and ovaries, gingiva, bone and the hair follicles of the skin. Transgene expression is developmentally regulated. | ||
| 006430 | B6Ei.129(Cg)-Figlatm1Dean/EiJ | Cryopreserved - Ready for recovery |
| Heterozygous female and homozygous male mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Female homozygous mice are infertile due to absence of primordial follicles in the ovary. No gene product (mRNA) is detected by RNase protection analysis of total ovarian RNA. This mutant mouse strain may be useful in studies of female infertility. | ||
| 007041 | B6Ei.129P2-Nr5a1tm2Klp/EiJ | Cryopreserved - Ready for recovery |
| Mice homozygous for this floxed allele are viable and fertile. These mutant mice have loxP sites flanking the C-terminal coding exon. When bred to Cre-recombinase expressing mice, offspring will have a deletion of this exon in the cre expressing tissue(s). These floxed mice may be useful in studying steroidogenic factors and pituitary gonadotrope function.
For example, when crossed to a strain expressing Cre recombinase in the anterior and intermediate lobes of the pituitary gland (see Stock No. 004426), this mutant mouse strain may be useful in studies of pituitary gonadotrope function. | ||
| 006305 | B6Ei.Cg-Nr0b1tm1.1Lja Tg(Sry)2Ei Chr YAKR/EiJ | Cryopreserved - Ready for recovery |
| 002021 | B6Ei.LT-Y(IsXPAR;Y)Ei/EiJ | Cryopreserved - Ready for recovery |
| This strain contains an abnormal LT/Sv-derived Y Chromosome comprising a spontaneous rearrangement (Eicher et al. 1991), formally designated Y(IsXPAR;Y)Ei and informally called Y*. Burgoyne et. al, (1998), updated the original description of the cytogenetic changes in this abnormal Y Chromosome, as an end-to-end fusion of two pseudoautosomal regions (PAR). Males with this abnormal Y Chromosome (XY*) are fully fertile and can be used to transmit this chromosomal aberration. Duplication of the PAR in Y* males permits generation of X and Y reciprocal translocation products during meiosis (see figure 1 in Eicher et.al 1991). Sperm produced by XY* males contain either a normal X Chromosome, the intact, complete Y*, a large marker sex chromosome comprising an X Chromosome and most of the Y (Y*), or a tiny cytogenetic marker sex chromosome (YX). Among offspring are the following genotypes and corresponding phenotypes: XY*, which are fu ..... For more information please see the full phenotype on the strain data sheet | ||
| 002605 | B6Ei.MA-A Chr YMA/MyJ/EiJ | Cryopreserved - Ready for recovery |
| This consomic is valuable in the study of sex determination. The TOrl /+ XYMA/MyJ offspring, from the cross of B6.Cg-TOrl /+ females with C57BL/6JEi-Chr YMA/MyJ/EiJ males, are sex reversed, with ovarian tissue development. | ||
| 008870 | C57BL/6-Tg(Hspa2-cre)1Eddy/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for the Hspa2-Cre transgene are viable and fertile, with expression of Cre recombinase directed to the developing male germ cells by the mouse Hspa2 (heat shock protein 2 [Hsp70-2]) promoter. The Hspa2-Cre sequences of the transgene are flanked by Acrv1 (acrosomal vesicle protein 1 [SP-10]) insulator elements (which are believed to tether the Acrv1 gene to the nuclear matrix in somatic cells [preventing transcription]). When these transgenic mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination is expected to result in deletion of the floxed sequences in leptotene/zygotene spermatocytes of the male offspring. The donating investigator reports that expression of Hspa2-Cre may be "leaky" and recombination of loxP-flanked sequences can occur in some cells in somatic tissues. These Hspa2-Cre mice may be useful to generate conditional mutations for studying mammalian spermatocyte development, g ..... For more information please see the full phenotype on the strain data sheet | ||
| 007265 | C57BL/6-Tg(Sry-EGFP)92Ei Chr YAKR/J/EiJ | Cryopreserved - Ready for recovery |
| 007264 | C57BL/6-Tg(Sry-EGFP)92Ei Tg(Sry)4Ei Chr YPOS/EiJ | Cryopreserved - Ready for recovery |
| 003927 | C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ | Cryopreserved - Ready for recovery |
| Tg(Sry-EGFP)92Ei has been shown to drive expression of GFP in pre-Sertoli and pre-granulosa cells of the genital ridge, with expression stronger in homozygotes than hemizygotes. Gonadal expression is not found in germ cells or vascular endothelial cells, but is restricted to a subset of somatic cells. Expression in the genital ridge begins in the center and moves out to the poles, with a bias toward the caudal pole. GFP expression is also found in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males, but not females, with lower levels of expression observed sparsely throughout the cortex. Strongest expression in the male brain is in the pars compacta and, at the cellular level, both cytoplasmic and nuclear localization are found. | ||
| 007250 | C57BL/6JEi-Chr YAKR/J/Ei | Cryopreserved - Ready for recovery |
| This consomic is valuable in the study of sex determination. The TOrl /+ XYAKR/J offspring, from the cross of B6.Cg-TOrl /+ females with C57BL/6JEi-Chr YAKR/J/EiJ males, are sex reversed, with ovarian tissue development. | ||
| 002890 | C57BL/6JEi-Chr YAPP/EiJ | Cryopreserved - Ready for recovery |
| 002604 | C57BL/6JEi-Chr YRF/J/EiJ | Cryopreserved - Ready for recovery |
| This consomic is valuable in the study of sex determination. The TOrl /+ XYRF offspring, from the cross of B6.Cg-TOrl /+ females with C57BL/6JEi-Chr YRF/J males, are sex reversed, having ovarian tissue development. | ||
| 001574 | C57BL/6JEi-Chr YWSB/Ei/EiJ | Cryopreserved - Ready for recovery |
| This consomic is valuable in the study of sex determination. The TOrl /+ XYWSB offspring, from the cross of B6.Cg-TOrl /+ females with C57BL/6JEi-Chr YWSB/EiJ males, are sex reversed, with ovarian tissue development. | ||
| 002063 | C57BL/6JEi-Tg(Sry)1Ei Chr YAKR/J/EiJ | Cryopreserved - Ready for recovery |
| In this strain, XX mice that carry the Tg(Sry)1Ei transgene are sex-reversed males. XYAKR/J mice heterozygous for TOrl on a C57BL/6J background develop as sex reversed females, but in the presence of the Tg(Sry)1Ei transgene these XY mice develop as normal males with testes. | ||
| 002708 | C57BL/6JEi-Tg(Sry)2Ei Chr YAKR/J/EiJ | Cryopreserved - Ready for recovery |
| In this strain, XX mice that carry the Tg(Sry)2Ei transgene are sex-reversed males. | ||
| 008337 | CBA/Ca-Tg(H2-K-HLA-G,B2M)1Alm/CmwJ | Cryopreserved - Ready for recovery |
| HLA-G transgenic (HLA-Gtg) mice are viable, fertile, and harbor two co-injected transgenes; H-2Kb/HLA-G and hβ2m. It was found that co-injecting the hβ2m transgene greatly facilitated expression of the whole HLA-G molecule, implying that endogenous mouse β2m cannot substitute for its human homolog. In addition, the CBA/Ca genetic background has a spontaneous deletion of the genes that encode the structurally/functionally homologous mouse Qa-2 protein, thus experimental results obtained using these HLA-Gtg mice may be attributed directly to transgene expression. Expression of HLA-G expression is observed in pre-implantation embryos and a variety of tissues; expression in the reproductive organs (uterus, ovary, and testes), lung, and liver is much more similar to mouse expression of Qa-2 than to Kb (despite the presence of the H-2Kb promoter and downstream coding sequence), while HLA-G expression in spleen, ..... For more information please see the full phenotype on the strain data sheet | ||
| 007898 | CBy.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this ROSA26-EGFP BAC transgene are viable and fertile, with widespread EGFP expression in all organs and tissues analyzed. In addition, stable and uniform EGFP expression is observed in differentiated progeny of transplanted hematopoietic stem cells (HSC) isolated from ROSA26-EGFP mice. These ROSA26-EGFP BAC transgenic mice may be useful for fluorescent labeling of cells and tissues both in vivo and in vitro, are suitable for use in histological preparations, and are appropriate as a source of fluorescently labeled donor cell populations (such as tracking hematopoietic cell fate in transplant recipients).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as publish ..... | ||
| 006180 | CD10/JlsJ | Cryopreserved - Ready for recovery |
| Swiss derived CD-1 mice are less responsive to exogenous estradiol than most inbred strains as measured by resistance to decreases in testes weight, spermatogenesis, and spermatid development. Derived from CD-1, the inbred strains CD3/JlsJ, CD7/JlsJ, and CD9/JlsJ mice exhibit a moderate sensitivity to endocrine disruption by estradiol, while CD10/JlsJ mice are highly resistant to endocrine disruption by estradiol. All lines generated in the selection program are significantly less responsive to estradiol than C57BL/6 mice. The selectively bred strains CD3/JlsJ (Stock No. 006177), CD7/JlsJ (Stock No. 006178), CD9/JlsJ (Stock No. 006179) and CD10/JlsJ (Stock No. 006180) may serve as tools for mapping and characterizing genes responsible for estradiol sensitivity.
SNP data is available for this strain on the ..... | ||
| 006177 | CD3/JlsJ | Cryopreserved - Ready for recovery |
| Swiss derived CD-1 mice are less responsive to exogenous estradiol than most inbred strains as measured by resistance to decreases in testes weight, spermatogenesis, and spermatid development. Derived from CD-1, the inbred strains CD3/JlsJ, CD7/JlsJ, and CD9/JlsJ mice exhibit a moderate sensitivity to endocrine disruption by estradiol, while CD10/JlsJ mice are highly resistant to endocrine disruption by estradiol. All lines generated in the selection program are significantly less responsive to estradiol than C57BL/6 mice. The selectively bred strains CD3/JlsJ (006177), CD7/JlsJ (006178), CD9/JlsJ (006179) and CD10/JlsJ (006180) may serve as tools for mapping and characterizing genes responsible for estradiol sensitivity. SNP data is available for this strain on the For more information please see the full phenotype on the strain data sheet | ||
| 006178 | CD7/JlsJ | Cryopreserved - Ready for recovery |
| Swiss derived CD-1 mice are less responsive to exogenous estradiol than most inbred strains as measured by resistance to decreases in testes weight, spermatogenesis, and spermatid development. Derived from CD-1, the inbred strains CD3/JlsJ, CD7/JlsJ, and CD9/JlsJ mice exhibit a moderate sensitivity to endocrine disruption by estradiol, while CD10/JlsJ mice are highly resistant to endocrine disruption by estradiol (personal communication). All lines generated in the selection program are significantly less responsive to estradiol than C57BL/6 mice. The selectively bred strains CD3/JlsJ (Stock No. 006177), CD7/JlsJ (Stock No. 006178), CD9/JlsJ (Stock No. 006179) and CD10/JlsJ (Stock No. 006180) may serve as tools for mapping and characterizing genes responsible for estradiol sensitivity.
SNP data is available for t ..... | ||
| 006179 | CD9/JlsJ | Cryopreserved - Ready for recovery |
| Swiss derived CD-1 mice are less responsive to exogenous estradiol than most inbred strains as measured by resistance to decreases in testes weight, spermatogenesis, and spermatid development. Derived from CD-1, the inbred strains CD3/JlsJ, CD7/JlsJ, and CD9/JlsJ mice exhibit a moderate sensitivity to endocrine disruption by estradiol, while CD10/JlsJ mice are highly resistant to endocrine disruption by estradiol (personal communication). All lines generated in the selection program are significantly less responsive to estradiol than C57BL/6 mice. The selectively bred strains CD3/JlsJ (Stock No. 006177), CD7/JlsJ (Stock No. 006178), CD9/JlsJ (Stock No. 006179) and CD10/JlsJ (Stock No. 006180) may serve as tools for mapping and characterizing genes responsible for estradiol sensitivity.
SNP data is available for t ..... | ||
| 010548 | D1.FVB(Cg)-Tg(CAG-luc,-GFP)L2G85Chco/FathJ | Cryopreserved - Ready for recovery |
| These L2G85.DBA/1 mice harbor the CAG-luc-eGFP L2G85 transgene. Homozygous mice are viable and fertile, with widespread expression of firefly luciferase and enhanced green fluorescent protein directed by the CAG promoter (human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter). Bioluminesence is detected in heart, spleen, muscle, pancreas, skin, thymus and bone marrow. Luciferase activity is not detected in mature erythrocytes, although low levels are detected in erythrocyte precursors and varying levels of activity in all leukocyte subsets tested. Homozygotes have no reported gross physical or behavioral abnormalities. Following luciferin injection, luciferase expression is generally greater in males than females. GFP fluorescence is detected in skin (upper epidermal layers) by fluorescence microscopy. The Donating Investigator reports that for the FVB-Tg(CAG-luc,-GFP)L2G85Chco/J (STOCK#8450) strain, no GFP fluorescence ..... For more information please see the full phenotype on the strain data sheet | ||
| 007042 | D2.129P2(B6)-Nr5a1tm2Klp/EiJ | Cryopreserved - Ready for recovery |
| Mice homozygous for this floxed allele are viable and fertile. These mutant mice have loxP sites flanking the C-terminal coding exon. When bred to Cre-recombinase expressing mice, offspring will have a deletion of this exon in the cre expressing tissue(s). These floxed mice may be useful in studying steroidogenic factors and pituitary gonadotrope function.
For example, when crossed to a strain expressing Cre recombinase in the anterior and intermediate lobes of the pituitary gland (see Stock No. 004426), this mutant mouse strain may be useful in studies of pituitary gonadotrope function. | ||
| 010947 | FVB-Tg(Gstm5-EGFP)1Ilis/J | Cryopreserved - Ready for recovery |
| Mice harboring the Gstm5-EGFP transgene are viable and fertile, normal in size and do not display any gross physical or behavioral abnormalities. The mouse glutathione S-transferase, mu 5 (Gstm5) promoter directs expression of enhanced green fluorescent protein (EGFP) to testis. Expression is not detected in liver, brain or kidney. Expression of EGFP does not completely parallel GSTM5. EGFP is widely distributed throughout the cytoplasm of spermatocytes, round spermatids and spermatozoa, but not spermatogonia, Sertoli cells or other cells, and is present from the pachytene spermatocytes to mature spermatozoa. | ||
| 012563 | FVB.129(Cg)-Slc9a3tm1Ges/J | Cryopreserved - Ready for recovery |
| These mice harbor a targeted mutation of the Na+/H+ exchanger isoform 3 locus (Nhe3 or Slc9a3) that abolishes endogenous gene expression. While no full-length mRNA is detected in kidney or intestine of homozygous mice, a truncated mutant mRNA lacking codons 320-831 (encoding sequences required for Na+/H+ exchange) is observed but expected to impart no dominant negative effects. When maintained as congenic on the FVB/N genetic background, homozygous mice exhibit a high mortality rate beginning just after weaning, with ~30% surviving to adulthood. Homozygous females are fertile, but homozygous males are infertile.
Homozygous (Nhe3-null) mice lack Na+/H+ exchanger isoform 3 function, and exhibit impaired intestinal absorption; resulting in severe diarrhea, altered salt and water homeostasis, and increased luminal fluid throughout the intestinal tract. Nhe3-null mice have increased PCNA-positive cells in the cryp ..... For more information please see the full phenotype on the strain data sheet | ||
| 007483 | FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ | Cryopreserved - Ready for recovery |
| On an albino background the X-linked transgene Tg(Tyr)3412ARpw permits visual identification of gender as early as embryonic day 10.5. This strain is segregating for Tg(Tyr)3412ARpw and homozygous for Tyrc-2J so the individuals not carrying Tg(Tyr3412)ARpw are albino. Because Tg(Tyr)3412ARpw inserted into the X Chromosome, breeding a carrier male with a noncarrier (wild-type) female results in embryos in which all XX individuals develop eye pigment, due to the Tg(Tyr)3412ARpw inherited from their father, while all XY individuals have non-pigmented eyes, having inherited a wild-type X Chromosome from their mother.
This strain is also homozygous for Tg(SryEGFP)92Ei. This reporter transgene consists of a 5' regulatory segment of the Sry gene driving EGFP. This transgene is expressed in the pre-support cell lineage (pre-sertoli and pre-granulosa cells) of the fetal genital ridge (Albrecht and Eicher, 2001) and in discrete areas the adult male but not female ..... | ||
| 009354 | FVB/N-Tg(Dazl-EGFP)10Rarp/J | Cryopreserved - Ready for recovery |
| Mice harboring the Dazl-eGFP transgene are viable and fertile, with the mouse Dazl (deleted in azoospermia-like) promoter/enhancer regions directing expression of enhanced green fluorescent protein (EGFP) primarily to adult mouse testis. Specifically, robust EGFP expression (both transcript and fluorescence) is observed in adult mouse testis in a developmentally-regulated, stage-specific expression pattern during spermatogenesis. Much lower EGFP transcript and fluorescence is observed in adult ovaries. Very low levels of EGFP transcript expression (but not fluorescence) is reported in fetal and newborn gonads of both sexes. These Dazl-eGFP transgenic mice may be useful for gametogenesis studies, including pachytene spermatocytes. | ||
| 002437 | FVB/N-Tg(MMTV-Notch4)3Rnc/J | Cryopreserved - Ready for recovery |
| Male mice transgenic for the Notch4 gene (previously called Int3) are sterile, and females fail to lactate. Mammary tissue of females does not develop completely, exhibiting dramatic inhibition of alveolar-lobular development and reduced penetration of the mammary fat pad by ductal epithelium. Glandular epithelia of tissues expressing the activated form of Notch4 generally display severe ductal hyperplasia. Salivary glands fail to differentiate completely. Male transgenic mice exhibit severe epididymal hyperplasia, which is thought to be the cause of their sterility. Both male and female mice develop focal adenomas of the mammary and salivary glands. | ||
| 006875 | FVB/N-Tg(Tagln-rtTA)E1Jwst/J | Cryopreserved - Ready for recovery |
| Transgenic SM22-rtTA mice are viable and fertile. These mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the murine SM22-alpha (SM22α or transgelin) promoter. When hemizygotes are mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in the bitransgenic offspring is inducible in smooth muscle cells with administration of the tetracycline analog, doxycycline. These SM22-rtTA mice provide a "Tet-On" tool that allows the inducible expression of genes in smooth muscle cells. | ||
| 003377 | FVB/N-Tg(Zp3-cre)3Mrt/J | Cryopreserved - Ready for recovery |
| The strain originated on an FVB/N background and is currently on the same background. The investigator maintains the strain by breeding hemizygotes. Homozygotes are subfertile. This is a transgenic line in which cre expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene, which is normally expressed exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain would be useful for deleting a floxed sequence specifically in the female germ line. | ||
| 007925 | STOCK En2tm5.1Alj/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this En2flox-taulacZ (also called En2-floxLacZ, En2tau-lacZ, or En2-tau-lacZ) mutation are viable but may not breed well (reported as "semi-fertile"). These mice harbor a loxP-flanked tau β-galactosidase "knock-in" allele that also abolishes engrailed 2 (En2) gene function. While both the tau-lacZ and En2 transcripts are made, only tau-lacZ is translated to protein. Expression of lacZ mimics that of the endogenous En2 gene. When bred to mice that express Cre recombinase, the resulting offspring will have the lacZ reporter deleted in the cre-expressing tissue(s) with subsequent restoration of En2 translation. These En2flox-taulacZ mutant mice may be useful for conditional reporter protein expression in En2-expressing tissues (including the early mesencephalon and rhombomere 1, and developing cerebellum and jaw muscles), as well as for conditional restoration of ..... For more information please see the full phenotype on the strain data sheet | ||
| 005994 | STOCK Mbtps1tm1Jdh/J | Cryopreserved - Ready for recovery |
| These mice carry a targeted mutation in which exon 2 of the targeted gene is flanked by loxP sites. A loxP-flanked ("floxed") neomycin resistance cassette also is inserted downstream in intron 2. Homozygotes are viable and fertile, and the floxed gene appears to function normally. When homozygotes are crossed with transgenic strains expressing Cre-recombinase, cre-mediated recombination of the loxP-flanked sequences can result in one of three genotypes: a) deletion of the neo cassette only, leaving a loxP-flanked second exon and unimpaired endogenous gene function. b) Deletion of exon 2 only, leaving a loxP-flanked neo cassette and no endogenous gene function. c) Deletion of both the neo cassette and exon 2, leaving a single loxP site and no endogenous gene function. When these floxed mutant mice are bred to mice carrying the Mx1-cre transgene (for example, Stock No. 003556), liver- ..... For more information please see the full phenotype on the strain data sheet | ||
| 008469 | STOCK Wnt9btm1.2Amc/J | Cryopreserved - Ready for recovery |
| Mice homozygous for the Wnt9bc allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). Such deletion is predicted to result in out-of-frame splicing of exon 1 to exon 3, and consequently a mutant transcript that would encode a nonfunctional peptide comprising the first 27 amino acids of Wnt9b that includes the signal peptide. These Wnt9bc mice may be useful in generating conditional mutations for studying the role of Wnt9b (and other Wnt family members) in development and canonical Wnt signaling cascades, including metanephric kidney and urogenital system development. | ||
| 007896 | STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this ROSA26-EGFP BAC transgene are viable and fertile, with widespread EGFP expression in all organs and tissues analyzed. In addition, stable and uniform EGFP expression is observed in differentiated progeny of transplanted hematopoietic stem cells (HSC) isolated from ROSA26-EGFP mice. These ROSA26-EGFP BAC transgenic mice may be useful for fluorescent labeling of cells and tissues both in vivo and in vitro, are suitable for use in histological preparations, and are appropriate as a source of fluorescently labeled donor cell populations (such as tracking hematopoietic cell fate in transplant recipients). | ||
| 002858 | STOCK Tg(Nes-cre)1Wme/J | Cryopreserved - Ready for recovery |
| These transgenic mice carry the Cre recombinase gene under the control of the rat nestin promoter. When crossed with a strain carrying a gene flanked by loxP sites, partial deletion of the loxP-flanked allele occurs before embryonic day 10.5 and is detectable in all adult organs examined including germ line cells. Because of the partial deletion, these balancer1 cre transgenic mice, as well as the balancer2 cre transgenic mice (Stock No. 002859), may be used to generate mosaic mice consisting of cells that are either wildtype or mutant for the gene of interest. The proportion of cells that carry the mutant allele varies between tissues and between individual mice. In cases where deletion of a particular gene is lethal, the generation of mosaic mice using these strains can bypass lethality in some mosaic individuals. The balancer2 line induces more variable deletion of loxP-flanked genes than the ..... For more information please see the full phenotype on the strain data sheet | ||
| 002859 | STOCK Tg(Nes-cre)2Wme/J | Cryopreserved - Ready for recovery |
| These transgenic mice carry the Cre recombinase gene under the control of the rat nestin promoter. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing cre, generating a proportion of mosaic animals. All adult organs, including germ-line cells, may be affected. The balancer2 line of transgenic mice show greater variability and instability in their generation of mosaics than the balancer1 line (Stock No. 002858). This variability of the balancer2 line can be of particular benefit if the gene of interest is vital and mosaic individuals are used to bypass lethality. | ||
| 008119 | STOCK Tg(Neurog3-cre/Esr1*)1Dam/J | Cryopreserved - Ready for recovery |
| These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse neurogenin 3, Neurog3, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in islet cells of the pancreas, undifferentiated spermatogonia and other Neurog3 expressing cells. Transgene expression closely patterns endogenous gene expression as analyzed by in situ hybridiza ..... For more information please see the full phenotype on the strain data sheet | ||
| 008579 | STOCK Tg(PSCA-EGFP)1Witt/J | Cryopreserved - Ready for recovery |
| The human prostate stem cell antigen promoter drives expression of the enhanced green fluorescent protein (EGFP) in these transgenic mice. Expression is detected in mid-gestation following the appearance of prostatic buds from the urogenital sinus. In adult mice, EGFP expression is restricted to a subset of cells located in the distal tips of the prostate gland towards the urethra. GFP expression increases during puberty and regeneration driven by androgen which are associated with expansive growth of the prostate. This strain may be a useful tool for studies of prostate epithelial cell subpopulations and pathways that involve this gene. | ||
| 012278 | STOCK Tg(Piwil1)1Ghan/J | Cryopreserved - Ready for recovery |
| This FLAG- and hemagglutinin (HA)-tagged Piwil1 (piwi-like homolog 1 (Drosophila); also known as Miwi) transgenic line enables researchers to selectively purify piwi-interacting RNA (piRNA) complexes using antibody-coated beads specific for the tagged elements. Expression, under the control of the transgene's native promoter, reproduces the developmentally timed pattern and subcellular localization of the endogenous protein. It begins at approximately postnatal day 14 (P14) in the pachytene stage of male germ cell meiosis and continues to adulthood. This strain may be helpful in studies of stem cell differentiation and spermatogenesis. | ||
| 012275 | STOCK Tg(Piwil2)1Ghan/J | Cryopreserved - Ready for recovery |
| This FLAG- and hemagglutinin (HA)-tagged Piwil2 (piwi-like homolog 2 (Drosophila); also known as Mili) transgenic line enables researchers to selectively purify piwi-interacting RNA (piRNA) complexes using antibody-coated beads specific for the tagged elements. Expression, under the control of the transgene's native promoter, reproduces the developmentally timed pattern and subcellular localization of the endogenous protein. It begins at approximately embryonic day 12.5 (E12.5) and protein is continuously present in germ cells until the haploid round spermatid stage in adults. This strain may be helpful in studies of stem cell differentiation and spermatogenesis. | ||
| 002395 | STOCK Tg(Zfy1-lacZ)218Bri/J | Cryopreserved - Ready for recovery |
| LacZ staining is present in the embryonic gonad of both male and female mice (somatic cells, but not germ cells). Staining is less intense in males and is repressed shortly after formation of the testis cords. Staining is also present in the kidney, brain, and spine prior to birth. | ||
| 006129 | STOCK Tg(Zp3-EGFP)1Dean/J | Cryopreserved - Ready for recovery |
| Hemizygous mice are viable and fertile with no gross phenotypic abnormalities. These mice express the Zp3-EGFP fusion protein under the direction of the Zp3 promoter. As such, EGFP is readily detected throughout the width of the zona pellucida surrounding growing oocytes, with no expression in the surrounding somatic cells. Mutant mice have normal synthesis, intracellular trafficking, and secretion/incorporation into the zona pellucida matrix of the transgenic fusion protein. These mice may be useful in studies of reproductive biology, including intracellular trafficking of zona pellucida proteins to the cell surface, oocyte development, fertilization research, as well as developmental biology. | ||
| 003275 | STOCK Tg(tetL)1Bjd/J | Cryopreserved - Ready for recovery |
| STOCK Tg(tetL)1Bjd/J transgenic mice are viable, fertile, and do not display an overt phenotype. These transgenic mice carry the luciferase gene coupled to a tetracycline-responsive promoter element (TRE). When mated to a strain expressing the tetracycline-controlled activator proteins tTA or rtTA coupled to a tissue-specific promoter, luciferase will be expressed in the appropriate tissue in a tetracycline-inducible fashion. | ||
| 003274 | STOCK Tg(tetNZL)2Bjd/J | Cryopreserved - Ready for recovery |
| In this strain both the lacZ gene with a nuclear localization signal (NZ) and the luciferase gene (L) are driven by a bidirectional, tetracycline-responsive promoter element (TRE). When mated to a strain expressing the tetracycline-controlled activator proteins tTA or rtTA coupled to tissue-specific promoters, lacZ and luciferase will be expressed in the appropriate tissue in a tetracycline-inducible fashion. According to the donating investigator, the expression of the two reporter genes is biased toward higher levels of lacZ than of luciferase, which, although low, is still measurable. | ||
| 008168 | STOCK Tg(tetO-DTA)1Gfi/J | Cryopreserved - Ready for recovery |
| These tet-DTA transgenic mice express diphtheria toxin A (DTA) under the control of a tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator) and a cytomegalovirus minimal promoter. When bred with another mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue diphtheria toxin A expression can be regulated with the tetracycline analog doxycycline (dox) in the resulting double mutant offspring. These tet-DTA mice may be useful in generating bi-transgenic mutant mice for the reversible, inducible deletion of specific groups of cells. For example, when bred to a strain expressing tTA in cardiac myocytes (see Stock No. 003170 for example), this mutant mouse strain may be useful in studies of human cardiomyopathies. | ||
| 016224 | B6.129S(Cg)-Id2tm2.1Blh/ZhuJ | Under Development - Now Accepting Orders |
| The Id2-EGFP knockin allele was designed to both abolish inhibitor of DNA binding 2 (Id2) gene function and express enhanced green fluorescent protein (eGFP) from the Id2 promoter/enhancer elements. No mRNA or protein expression from the Id2-eGFP allele is observed. The donating investigator reports that homozygote mice mice are runted with defective lung alveolarization. Other organ systems have not been evaluated. However, Id2-eGFP homozygotes may be expected to exhibit the same phenotype as mice homozygous for other null mutations of this gene (including postnatal lethality and defects of the immune system, digestive tract, kidneys, adipose tissue and mammary gland development). The donating investigator also reports eGFP expression recapitulates the endogenous Id2 expression pattern. In the lungs, immunohistochemical detection of eGFP recapitulates the epithelial expression of the endogenous gene (distal tip lung epithelial multipotent precursor cells of the ..... For more information please see the full phenotype on the strain data sheet | ||
| 016092 | B6.129S4-Git1Gt(FHCRC-GT-S10-12C1)Sor/WeisJ | Under Development - Now Accepting Orders |
| A targeting vector containing β-galactosidase, a polyadenylation signal, and a PGK Hygromycin selection cassette, was randomly inserted downstream of exon 1 of the endogenous G protein-coupled receptor kinase-interactor 1 (Git1) gene. Heterozygous mice are viable, normal in size and do not display any gross physical or behavioral abnormalities, although some males have decreased fertility. Homozygous mice die within a few days after birth. This β-geo secretory trap mutation abolishes endogenous gene function and expresses a Git1-exon1/lacZ fusion protein. Git1 belongs to the family of ADP-ribosylation factor GTPase-activating proteins (ARF-GAP) and has been implicated in the regulation of G protein-coupled receptor sequestration, cell migration, neuronal spine formation, and aggregate formation in Huntington's disease. GIT1 expression is restricted to some areas of the brain, to cells lining blood vessels, bronchi, and bile ducts. Expression of GIT1 is absent ..... For more information please see the full phenotype on the strain data sheet | ||
| 017593 | B6;129S-Sox2tm1(cre/ERT2)Hoch/J | Under Development - Now Accepting Orders |
| These Sox2-CreER knockin mice have the SRY-box containing gene 2 (Sox2) open reading frame replaced with a CreERT2 fusion gene. Sox2 is a widespread marker of pluripotent and many adult stem/progenitor cell types. Heterozygous mice are viable and fertile, while homozygous mice exhibit embryonic lethality. Following tamoxifen administration, Cre-ERT2 activity is observed in adult epithelial tissues; including testes, forestomach, glandular stomach, anus, cervix, esophagus, and lens, as well as glands associated with oral cavity, trachea, and cervix. When these mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the cre-expressing cells of the offspring. Cre-ERT2 activity is also detected in blastocysts, neural progenitor cells, and embryonic stem cell cultures following tamoxifen administration. These mice may be useful for stud ..... For more information please see the full phenotype on the strain data sheet | ||
| 017337 | B6;129S4-Mirc5tm1Jae/J | Under Development - Now Accepting Orders |
| Approximately 75% of homozygotes die before birth of unknown causes. The surviving homozygous females are sterile. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot or real-time RT-PCR analysis of homozygous knockout ES cells lines produced from mutant mice. Homozygous embryos exhibit development delays as early as embryonic day 8.5, and loss of homozygous embryos occurs E11.5 to E18.5. Approximately 16% of homozygous embryos, at E10.5 or younger, are partially or completely outside the yolk sac. Embryonic primordial germ cell migration is mislocalized. A reduced number of gonocytes/germ cells in homozygotes is detectable as early as E11.5. Homozygous adult females have smaller size ovaries when compared to wildtype littermates. Ovaries from 5 to 8 week old homozygotes have few follicles, and in ovaries from homozygous fem ..... For more information please see the full phenotype on the strain data sheet | ||
| 016999 | B6;129S6-Gt(ROSA)26Sortm1(xstpx-rtTA2S*M2)Whsu/J | Under Development - Now Accepting Orders |
| These mice contain the rtTAS*M2, or rtTA2S-M2, an optimized form of reverse tetracycline regulated transactivator, gene inserted into the Gt(ROSA)26Sor locus. Expression of the rtTAS*M2 is blocked by a loxP-flanked STOP fragment placed between the rtTAS*M2 sequence and the Gt(ROSA)26Sor promoter. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will express rtTAS*M2, reverse tetracycline regulated transactivator, in the cre expressing cells. When bred to mice that also carry a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the gene of interest may be regulated by the tetracycline analog, doxycycline (dox). In the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not disp ..... For more information please see the full phenotype on the strain data sheet | ||
| 014547 | FVB/N-Tg(tetO-Fasl)BDepa/J | Under Development - Now Accepting Orders |
| Mice hemizygous for the (tetOp)7-FasL transgene (TetOp-FasL transgenic mice) are viable and fertile with no reported phenotypic abnormalities. The (tetOp)7-FasL transgene has the Tet response element (TRE or tetO) upstream of a murine Fas ligand (FasL) coding sequence. When bred with other mice expressing tetracycline-controlled transactivator protein (tTA) or reverse tetracycline-controlled transactivator protein (rtTA), FasL overexpression in the resulting double mutant offspring can be regulated with tetracycline or its analog doxycycline (dox). FasL overexpression leads to Fas/FasL receptor-mediated death-signaling pathway activation and results in cell apoptosis.
The donating investigator reports that transgenic mice from founder line B (TetOp-FasL transgenic line B) exhibit very high FasL overexpression levels in the presence of rtTA and 0.01 mg/ml Dox (low FasL levels are achievable by titrating Dox even lower). The donating investigator rep ..... For more information please see the full phenotype on the strain data sheet | ||
| 014093 | STOCK Tg(tetO-CHRM3*)1Blr/J | Under Development - Now Accepting Orders |
| Hemizygous TRE-hM3Dq transgenic mice are viable and fertile, with no reported phenotypic abnormalities. The TRE-hM3Dq transgene has a modified Tet response element (TRE or tetO) upstream of the mutant G protein-coupled receptor, hM3Dq (a human muscarinic 3 receptor with two amino acid substitutions (Y149C3.33/A239G5.46) that abolish receptor affinity for the native ligand, acetylcholine (ACh), but allow receptor binding and subsequent activation by the small pharmacologically inert molecule clozapine-N-oxide (CNO)). When bred with another mouse expressing tetracycline-controlled transactivator protein (tTA) or reverse tetracycline-controlled transactivator protein (rtTA), hM3Dq expression in the resulting double mutant offspring can be regulated with tetracycline or its analog doxycycline (dox). As designed, TRE-hM3Dq transgenic mice have no reported levels of hM3Dq activity in tTA(+dox) or rtTA(-dox) cell types. In TRE-hM3Dq transgenic tTA(-dox) ..... For more information please see the full phenotype on the strain data sheet | ||
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Please indicate your interest in purchasing any of the strains listed below when they become available for distribution by checking the box next to the strain(s) of interest and then selecting the "Continue" button which leads to an Interest Form.View a Data sheet for New Strains Awaiting Transfer
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