Search Criteria: Research Area is "Research Tools: Reproductive Biology Research (Cre-lox System)"

New Strains Under Development

JAX^® Mice Strains

Stock Number	Strain Name   Strain Description	Standard Supply
007668	B6.129S4(Cg)-Arntltm1Weit/J	Repository- Live
	Mice homozygous for this conditional (floxed) allele possess loxP sites flanking exon 8 of the targeted gene and are viable and fertile, with circadian behavioral rhythms indistinguishable from wildtype littermates. When bred to mice that express Cre recombinase, the resulting offspring will have the exon encoding the ARNTL (BMAL1) basic helix-loop-helix (bHLH) domain deleted in the cre-expressing tissue(s). These Bmal1-floxed mutant mice may be useful in generating conditional mutations (whole-mouse or tissue-specific) to study the role of circadian clock/circadian rhythm in physiological and behavioral regulation. For example, when crossed to a strain expressing Cre recombinase in the retina (see Stock No. 005105), this mutant mouse strain may be useful in studies of the circadian clock of the retina.
005897	B6.129S4-Ppardtm1Rev/J	Repository- Live
	These mice possess loxP sites on either side of exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 4 of the targeted gene is deleted in the tissue of interest, leading to premature termination of the translation product upstream of the DNA binding domain. This strain may be useful in generating tissue-specific mutants of the floxed allele for use in studies including embryo development, adipocyte physiology, fat metabolism and storage, inflammation, and cancer.
006881	B6.Cg-Tg(Aqp2-cre)1Dek/J	Repository- Live
	Mice hemizygous for this AQP2-Cre transgene are viable and fertile. Transgenic cre activity, directed by the mouse aquaporin 2 promoter, is observed in kidney cells (collecting duct) and testes (sperm). When bred with mice containing a loxP-flanked sequence of interest, cre-mediated recombination will result in deletion of the flanked sequence. In such breedings, maternal inheritance of the transgene is recommended for kidney-specific recombinase activity as males express cre in sperm as well as kidney tissues. These AQP2-Cre mice may be used to generate conditional mutations in the renal collecting duct for studying nephrology, physiology, metabolism, or type II diabetes. In addition, cre expression in sperm may be useful in generating conditional mutations in multiple or all tissues in the resulting offspring.
007900	C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J	Repository- Live
	Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration. For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation.
003651	C57BL/6-Tg(Zp3-cre)93Knw/J	Repository- Live
	This is a transgenic line in which cre expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene. This promoter normally directs expression exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain would be useful for deleting a floxed sequence specifically in the female germ line. The Donating Investigator suggests to accomplish this, females homozygous or heterozygous for the floxed allele, as well as hemizygous for the Zp3cre allele are crossed with wild type males. Progeny will carry the deleted-floxed allele.
006954	FVB-Tg(Ddx4-cre)1Dcas/J	Repository- Live
	Mice hemizygous for this Vasa-Cre transgene are viable and fertile. Transgenic cre activity is directed to male and female germ cells starting at embryonic day (e)15-e18. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequence. In such breedings, occasional hemizygous mice may exhibit variegated cre expression in skin epithelium or global cre expression (<20% incidence). Differential parent-of-origin transgene expression is observed. When the mother harbors Vasa-Cre, virtually all progeny undergo global Cre-mediated recombination, even those that do not inherit the transgene (which may be useful in converting a "floxed" allele to a null while obviating the need to perform additional crosses to remove the transgene). To achieve germ-line specific Cre-mediated recombination in offspring, paternal Vasa-Cre mice should be used. These Vasa-Cre mice may be useful in generating c > ..... For more information please see the full descriiption on the strain data sheet
008119	STOCK Tg(Neurog3-cre/Esr1)1Dam/J	Repository- Live
	These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse neurogenin 3, Neurog3, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in islet cells of the pancreas, undifferentiated spermatogonia and other Neurog3 expressing cells. Transgene expression closely patterns endogenous gene expression as analyzed by in situ hybridiza ..... For more information please see the full descriiption on the strain data sheet
003552	B6129-Tg(Wap-cre)11738Mam/J	Repository-Cryopreserved
	This transgenic strain expresses P1 Cre recombinase under the control of the Wap (whey acidic protein) promoter. In mammary gland tissues, the Wap promoter directs expression to secretory epithelium. A maximum of Cre mediated recombination is achieved during pregnancy and lactation, but recombined cells are still present after involution and complete remodeling of the gland. Cre recombinase expression is not entirely restricted to mammary gland, however. A limited amount of Cre activity has been reported in brain tissue.
003466	B6;D2-Tg(Sycp1-cre)4Min/J	Repository-Cryopreserved
007041	B6Ei.129P2-Nr5a1tm2Klp/EiJ	Repository-Cryopreserved
	Mice homozygous for this floxed allele are viable and fertile. These mutant mice have loxP sites flanking the C-terminal coding exon. When bred to Cre-recombinase expressing mice, offspring will have a deletion of this exon in the cre expressing tissue(s). These floxed mice may be useful in studying steroidogenic factors and pituitary gonadotrope function. For example, when crossed to a strain expressing Cre recombinase in the anterior and intermediate lobes of the pituitary gland (see Stock No. 004426), this mutant mouse strain may be useful in studies of pituitary gonadotrope function.
003394	C57BL/6-Tg(Zp3-cre)3Mrt/J	Repository-Cryopreserved
	This is a transgenic line in which cre expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene. This promoter normally directs expression exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain would be useful for deleting a floxed sequence specifically in the female germ line. Homozygotes females produce small litters (3.7 pups/litter; Genesis 26:110-112, 2000). An alternative transgenic strain that bears a similar transgenic construct and produces larger litters (~6 pups/litter) is available: C57BL/6-Tg(Zp3-cre)93Knw/J (Stock No. 003651).
007042	D2.129P2(B6)-Nr5a1tm2Klp/EiJ	Repository-Cryopreserved
	Mice homozygous for this floxed allele are viable and fertile. These mutant mice have loxP sites flanking the C-terminal coding exon. When bred to Cre-recombinase expressing mice, offspring will have a deletion of this exon in the cre expressing tissue(s). These floxed mice may be useful in studying steroidogenic factors and pituitary gonadotrope function. For example, when crossed to a strain expressing Cre recombinase in the anterior and intermediate lobes of the pituitary gland (see Stock No. 004426), this mutant mouse strain may be useful in studies of pituitary gonadotrope function.
003377	FVB/N-Tg(Zp3-cre)3Mrt/J	Repository-Cryopreserved
	The strain originated on an FVB/N background and is currently on the same background. The investigator maintains the strain by breeding hemizygotes. Homozygotes are subfertile. This is a transgenic line in which cre expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene, which is normally expressed exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain would be useful for deleting a floxed sequence specifically in the female germ line.
005994	STOCK Mbtps1tm1Jdh/J	Repository-Cryopreserved
	These mice carry a targeted mutation in which exon 2 of the targeted gene is flanked by loxP sites. A loxP-flanked ("floxed") neomycin resistance cassette also is inserted downstream in intron 2. Homozygotes are viable and fertile, and the floxed gene appears to function normally. When homozygotes are crossed with transgenic strains expressing Cre-recombinase, cre-mediated recombination of the loxP-flanked sequences can result in one of three genotypes: a) deletion of the neo cassette only, leaving a loxP-flanked second exon and unimpaired endogenous gene function. b) Deletion of exon 2 only, leaving a loxP-flanked neo cassette and no endogenous gene function. c) Deletion of both the neo cassette and exon 2, leaving a single loxP site and no endogenous gene function. When these floxed mutant mice are bred to mice carrying the Mx1-cre transgene (for example, Stock No. 003556), liver- ..... For more information please see the full descriiption on the strain data sheet
002858	STOCK Tg(Nes-cre)1Wme/J	Repository-Cryopreserved
	These transgenic mice carry the Cre recombinase gene under the control of the rat nestin promoter. When crossed with a strain carrying a gene flanked by loxP sites, partial deletion of the loxP-flanked allele occurs before embryonic day 10.5 and is detectable in all adult organs examined including germ line cells. Because of the partial deletion, these balancer1 cre transgenic mice, as well as the balancer2 cre transgenic mice (Stock No. 002859), may be used to generate mosaic mice consisting of cells that are either wildtype or mutant for the gene of interest. The proportion of cells that carry the mutant allele varies between tissues and between individual mice. In cases where deletion of a particular gene is lethal, the generation of mosaic mice using these strains can bypass lethality in some mosaic individuals. The balancer2 line induces more variable deletion of loxP-flanked genes than the ..... For more information please see the full descriiption on the strain data sheet
002859	STOCK Tg(Nes-cre)2Wme/J	Repository-Cryopreserved
	These transgenic mice carry the Cre recombinase gene under the control of the rat nestin promoter. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing cre, generating a proportion of mosaic animals. All adult organs, including germ-line cells, may be affected. The balancer2 line of transgenic mice show greater variability and instability in their generation of mosaics than the balancer1 line (Stock No. 002858). This variability of the balancer2 line can be of particular benefit if the gene of interest is vital and mosaic individuals are used to bypass lethality.

(16 stocks)         Back to Top

New Strains Under Development

(See informational text following listing of strains)

How to Register Interest
Please indicate your interest in purchasing any of the strains listed below when they become available for distribution by checking the box next to the strain(s) of interest and then selecting the "Continue" button which leads to an Interest Form.

View a Data sheet for New Strains Under Development
Select the strain name to link to the strain data sheet.

	Stock Number	Strain Name   Strain Description	Standard Supply
	008569	129-Alpltm1(cre)Nagy/J	Under Development for Production
	This strain expresses Cre recombinase from the targeted locus. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in tissue-specific deletion of the flanked sequence. Recombination occurs primarily in embryonic primordial germ cells. Approximately 60% of gonadal cells isolated from embryonic day 13.5 embryos exhibit Cre recombinase activity. Mosaic ectopic recombinase activity does occur. Homozygotes are not viable. This mutant mouse strain represents a model that may be useful in studies of reproductive and endocrine systems development.
	007915	129S.FVB-Tg(Amh-cre)8815Reb/J	Under Development for Production
	Mice harboring the Amh-cre transgene are viable and fertile, with expression of Cre recombinase directed by the testis Sertoli cell-specific promoter elements of the anti-Mullerian hormone (Amh) gene. Cre-recombinase activity is reported in testis Sertoli cells during male sexual development as early as E14.5, with no evidence for cre expression detected in other tissues examined. When Amh-cre transgenic males are bred with female mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence specifically in testis Sertoli cells. These Amh-cre transgenic mice may be useful in generating conditional knockouts in testis Sertoli cells for studying male embryonic sexual differentiation and the regulation of spermatogenesis.
	008600	B6.129P2-Gt(ROSA)26Sortm1(tTA)Roos/J	Under Development for Production
	Mice heterozygous for the ROSA:LNL:tTA conditional mutation of the Gt(ROSA)26Sor locus are viable and fertile, with a loxP-flanked STOP cassette preventing transcription of a downstream optimized/modified tetracycline-controlled transactivator protein ("mtTA"). Applying both Cre-lox and Tet-Off technologies, these ROSA:LNL:tTA mutant mice may be useful to generate compound mutant mice in which expression of a tetracycline-responsive promoter element (TRE; also called tet-operator or tetO)-driven gene of interest can be both directed to the cell types defined by the chosen Cre recombinase expression, as well as turned off by the addition of tetracycline (or its analog doxycycline (dox)).
	008469	B6.129X1(Cg)-Wnt9btm1.2Amc/J	Under Development for Production
	Mice homozygous for the Wnt9bc allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). Such deletion is predicted to result in out-of-frame splicing of exon 1 to exon 3, and consequently a mutant transcript that would encode a nonfunctional peptide comprising the first 27 amino acids of Wnt9b that includes the signal peptide. These Wnt9bc mice may be useful in generating conditional mutations for studying the role of Wnt9b (and other Wnt family members) in development and canonical Wnt signaling cascades, including metanephric kidney and urogenital system development.
	007914	B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J	Under Development for Production
	Ai14 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE::deltaNeo conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. Ai14 mice are not expected to express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre is expected to be detectable by fluorescence and mRNA (in situ hybridization). These Ai14 mice are useful as a Cre reporter strain; expressing the red fluorescent protein variant, tdTomato, following Cre-mediated recombination.
	007920	B6.Cg-Gt(ROSA)26Sortm2(CAG-EYFP)Hze/J	Under Development for Production
	Ai2 mice hemizygous for this Rosa-CAG-LSL-EYFP conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream enhanced yellow fluorescent protein (EYFP). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of EYFP. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, EYFP expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai2 mice do not express EYFP prior to introduction of Cre recombinase and EYFP fluorescence following exposure to cre is weak but easily detected by mRNA (in situ hybridization) and antibody staining (immunohistochemistry). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-mediated ..... For more information please see the full description on the strain data sheet
	007903	B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J	Under Development for Production
	Ai3 mice hemizygous for this Rosa-CAG-LSL-EYFP-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream enhanced yellow fluorescent protein (EYFP). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of EYFP. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, EYFP expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai3 mice do not express EYFP prior to introduction of Cre recombinase and EYFP expression following exposure to cre can be detected by fluorescence, mRNA (in situ hybridization) and antibody staining (immunohistochemistry). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-medi ..... For more information please see the full description on the strain data sheet
	007906	B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J	Under Development for Production
	Ai6 mice hemizygous for this Rosa-CAG-LSL-ZsGreen1-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream enhanced green fluorescent protein (ZsGreen1). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of ZsGreen1. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, ZsGreen1 expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai6 mice do not express ZsGreen1 prior to introduction of Cre recombinase and ZsGreen1 expression following exposure to cre can be detected by fluorescence and mRNA (in situ hybridization). Bright fluorescence is observed mainly in cell bodies. Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the report ..... For more information please see the full description on the strain data sheet
	007909	B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J	Under Development for Production
	Ai9 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai9 mice do not express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre can be detected by fluorescence and mRNA (in situ hybridization). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-mediated recombination if ..... For more information please see the full description on the strain data sheet
	008208	FVB/N-Tg(Stra8-cre)1Reb/J	Under Development for Production
	Homozygous Stra8-cre transgenic mice are viable and fertile, with expression of an optimized variant of Cre recombinase (iCre) directed by the 1.4 kb mouse Stra8 (stimulated by retinoic acid gene 8) genomic promoter fragment. Postnatal Cre-recombinase expression is first observed in testes at postnatal day (P)3 (early-stage spermatogonia), increases out to P7 (pre-leptotene-stage spermatocytes), and is not detected in other tissues examined (including ovaries). In addition, no cre expression is reported in male or female embryos. When Stra8-cre transgenic males are bred with female mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence, specifically during these stages of spermatogenesis. These Stra8-cre transgenic mice may be useful in generating conditional knockouts in postnatal, premeiotic, male germ cells for studying spermatogenesis.

(10 stocks)         Back to Top

New Strains Under Development

• Receive periodic updates on the status of the colony UNDER DEVELOPMENT
• Obtain advance notification of strain availability and opportunity to order prior to the strain being published as available
• Provide input affecting speed and quantity of availability

The Jackson Laboratory serves as a worldwide distributor and national repository for common and rare strains of inbred mice and mice carrying spontaneous mutations or induced mutations (i.e., transgenic, targeted/"knockout", or chemically induced mutations). At any one time, we have over 100 strains at various stages of development and colony expansion. Strains "Under Development" fall into two categories depending on the anticipated demand from the scientific community.

1. Strains that will be made available from a live distribution colony at The Jackson Laboratory.
   These strains are designated as: "Under Development for Distribution Colony"
2. Strains that will be made available through the Cryopreservation Repository.
   These strains are designated as: "Under Development for Cryopreservation Repository"

It is VERY IMPORTANT that you register interest in strains Under Development. The anticipated demand for a strain enables us to determine effectively the distribution plan for each strain Under Development. Registering interest also provides benefits to you (including advance notification of pending availability). Whether a strain is made available from a live colony OR from our cryopreservation repository, you may want to consider the option of Dedicated Supply. To learn more about Dedicated Supply, go to Services.

Back to top

Send questions to our Technical Support team using the Express Technical Support Form.
(3.2)