Search Criteria: Research Area is "Research Tools: Cre-lox System"
| Stock Number |
Strain Name Strain Description |
Standard Supply |
| 003474 | B6.129S4-Gt(ROSA)26Sortm1Sor/J | Level 4 |
| Homozygous mice are viable and fertile, with a loxP-flanked DNA STOP sequence preventing expression of the downstream lacZ gene. When crossed with a cre transgenic strain, the STOP sequence is removed and lacZ is expressed in cells/tissues where cre is expressed. These mutant mice may be used as a Cre-reporter strain; to test the tissue/cellular expression pattern of cre transgenic mice. | ||
| 004337 | 129(Cg)-Foxg1tm1(cre)Skm/J | Repository- Live |
| This strain expresses Cre recombinase from the endogenous Foxg1 locus. Forkhead box G1 is required for telencephalon development and is expressed specifically in the telencephalon and discrete head structures. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in the telencephalon, anterior optic vesicle (developing lens and retina), otic vesicle, facial and head ectoderm, olfactory epithelium, mid-hindbrain junction and pharyngeal pouches. Mice that are homozygous for the targeted mutation die perinatally. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain represents a model that may be useful in studies of telencephalic development. | ||
| 008569 | 129-Alpltm1(cre)Nagy/J | Repository- Live |
| This strain expresses Cre recombinase from the targeted locus. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in tissue-specific deletion of the flanked sequence. Recombination occurs primarily in embryonic primordial germ cells. Approximately 60% of gonadal cells isolated from embryonic day 13.5 embryos exhibit Cre recombinase activity. Mosaic ectopic recombinase activity does occur. Homozygotes are not viable. This mutant mouse strain represents a model that may be useful in studies of reproductive and endocrine systems development. | ||
| 008669 | 129-Daxxtm2Led/J | Repository- Live |
| These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s). These Daxx (Fas death domain-associated protein) conditional (floxed) mutant mice may be useful in generating conditional mutations for studying apoptosis and regulation of gene transcription. | ||
| 004293 | 129-Shhtm2Amc/J | Repository- Live |
| Mice that are homozygous for the Shhtm2Amc targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This conditional mutant contains two loxP sites flanking exon 2 of the targeted allele. Cre-mediated recombination excises exon 2 and some surrounding intronic sequence, generating a null allele. When the conditional mutant is crossed with a ubiquitously-expressing Cre recombinase carrier to remove Shh activity in the early embryo, the resulting phenotype resembles the Shh null mutation. These conditional mutant mice may be mated to strains expressing Cre recombinase to study the effects of temporal and tissue-specific ablation of the targeted allele. This mutant mouse strain represents a model that may be useful in studies of developmental defects resulting from disruption of Shh-dependent pathways.
When bred to a strain expressing Cre recombinase under the control of a tet ..... | ||
| 003328 | 129-Tg(Prm-cre)58Og/J | Repository- Live |
| Mice homozygous for this PrmCre transgene are viable and fertile. Embryonic stem cells containing recombinase transgenes that are expressed in the male germ line, but not in other tissues or in the embryonic stem cells themselves, substantially simplify the production of subtle or conditional mutations in mice. This strain shows that transgenes comprised of the mouse protamine 1 promoter and the Cre recombinase coding sequence mediate the efficient recombination of a cre target transgene in the male germ line, but not in other tissues. This system can be used for reducing the time, effort, and resources required to produce homologously recombined alleles in mice that have been secondarily rearranged by site-specific recombinase. | ||
| 009084 | 129S-Mnttm1Awb/J | Repository- Live |
| These mice possess loxP sites flanking exons 4-6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 4 through 6 deleted, the loxP site flanked hygro resistance cassette deleted, or both excised in the cre-expressing tissue(s).
When bred to a strain with Cre recombinase expression in a wide range of tissues (see Stock No. 003314 for example), this mutant mouse strain may be useful in studies of Miller-Dieker syndrome, embryonic development and craniofacial defects. When bred to a strain with Cre recombinase expression in a wide range of tissues, especially mammary gland (see Stock No. 003553 for example), this mutant mouse ..... | ||
| 008396 | 129S-Top2btm2Jcw/J | Repository- Live |
| These mice possess loxP sites flanking the 3 exons encoding the active-site tyrosyl residue of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the 3 exons deleted in the cre-expressing tissue(s). When bred to a strain expressing Cre recombinase during spermatogenesis (see Stock No. 003328 for example), this mutant mouse strain may be useful in studies of neural development. When bred to a strain expressing Cre recombinase in the telencephalon and discreet head structures (see Stock No. 004337, 006084 for example), this mutant mouse strain may be useful in studies of corticogenesis. | ||
| 007179 | 129S.Cg-Tg(UBC-cre/ESR1)1Ejb/J | Repository- Live |
| Mice hemizygous for this Cre-ERT2 transgene are viable and fertile. Mice from this founder line have strong tamoxifen-inducible cre activity in all reported tissue types. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to OHT. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in widespread cel ..... For more information please see the full phenotype on the strain data sheet | ||
| 007915 | 129S.FVB-Tg(Amh-cre)8815Reb/J | Repository- Live |
| Mice harboring the Amh-cre transgene are viable and fertile, with expression of Cre recombinase directed by the testis Sertoli cell-specific promoter elements of the anti-Mullerian hormone (Amh) gene. Cre-recombinase activity is reported in testis Sertoli cells during male sexual development as early as E14.5, with no evidence for cre expression detected in other tissues examined. When Amh-cre transgenic males are bred with female mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence specifically in testis Sertoli cells. These Amh-cre transgenic mice may be useful in generating conditional knockouts in testis Sertoli cells for studying male embryonic sexual differentiation and the regulation of spermatogenesis. | ||
| 009581 | B6(129S4)-Et(cre/ERT2)1642Rdav/J | Repository- Live |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of the Cre-ERT2 fusion gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Cre recombinase activity is observed in brain tissues following tamoxifen administration. The donating investigators may not have assessed expression in tissues other than brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
The Cre-ERT2 fusion protein (Cre-ERT2) consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OH ..... | ||
| 009574 | B6(129S4)-Et(cre/ERT2)21Rdav/J | Repository- Live |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of the Cre-ERT2 fusion gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Cre recombinase activity is observed in brain tissues following tamoxifen administration. The donating investigators may not have assessed expression in tissues other than brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
The Cre-ERT2 fusion protein (Cre-ERT2) consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OH ..... | ||
| 009578 | B6(129S4)-Et(cre/ERT2)398Rdav/J | Repository- Live |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of the Cre-ERT2 fusion gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Cre recombinase activity is observed in brain tissues following tamoxifen administration (although some cre activity is reported prior to tamoxifen administration). The donating investigators may not have assessed expression in tissues other than brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
The Cre-ERT2 fusion protein (Cre-ERT2) consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind t ..... | ||
| 009573 | B6(129S4)-Et(cre/ERT2)4Rdav/J | Repository- Live |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of the Cre-ERT2 fusion gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Cre recombinase activity is observed in brain tissues following tamoxifen administration. The donating investigators may not have assessed expression in tissues other than brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
The Cre-ERT2 fusion protein (Cre-ERT2) consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OH ..... | ||
| 009579 | B6(129S4)-Et(cre/ERT2)837Rdav/J | Repository- Live |
| Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of the Cre-ERT2 fusion gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Cre recombinase activity is observed in brain tissues following tamoxifen administration. The donating investigators may not have assessed expression in tissues other than brain. When these enhancer trap transgenic mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.
The Cre-ERT2 fusion protein (Cre-ERT2) consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OH ..... | ||
| 009652 | B6.129(Cg)-Dag1tm2.1Kcam/J | Repository- Live |
| These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 2 of the targeted gene is deleted in the tissue of interest. | ||
| 007676 | B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J | Repository- Live |
| Mice homozygous for this mT/mG mutation are viable and fertile. These mice possess loxP sites on either side of a membrane-targeted tdTomato (mT) cassette and express strong red fluorescence in all tissues and cell types examined. Tail or whole body epifluorescence is sufficient to identify mT/mG homozygotes. When bred to Cre recombinase expressing mice, the resulting offspring have the mT cassette deleted in the cre expressing tissue(s), allowing expression of the membrane-targeted EGFP (mG) cassette located just downstream. The donating investigator reports that the ACTB promoter allows stronger and persistent expression of the fluorescent proteins (especially in adult cells) compared to the endogenous Gt(ROSA) locus alone. This double-fluorescent system allows direct live visualization of both recombined and non-recombined cells at single cell resolution, offering an internal control for phenotypic analysis of Cre-induced mosaic mutants and providing a second marker fo ..... For more information please see the full phenotype on the strain data sheet | ||
| 004178 | B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J | Repository- Live |
| These Z/EG transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being liver and lung tissue. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with enhanced GFP expression in tissues expressing Cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells.
As an example, when crossed to a strain expressing Cre recombinase in olfactory sensory neurons (see Stock No. 006668), this mutant mouse strain may be useful in lineage tracing. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved t ..... | ||
| 008471 | B6.129(SJL)-Oxtrtm1.1Wsy/J | Repository- Live |
| Mice homozygous for the Oxtrflox allele are viable and fertile, with loxP sites flanking exons 2-3 of the targeted gene. Expression and receptor binding distributions from the Oxtrflox targeted allele are reported to be normal when compared to wild-type. When Oxtrflox mice are bred to mice that express Cre recombinase, the resulting offspring will have the floxed region deleted in the cre-expressing tissue(s). These Oxtrflox mice may be used for spatial and temporal inactivation of the oxytocin receptor in studying (for example) parturition and lactation, as well as social, behavioral, and learning disorders such as autism and anxiety.
These Oxtrflox mice may also be useful along with the Oxt/EGFP AI03 transgenic mice (Stock No. 006043) or oxytocin targeted mutant mice (Stock No. 002713) from the same ..... | ||
| 005319 | B6.129-Cdh1tm2Kem/J | Repository- Live |
| These mice possess loxP sites flanking exons 6 to 10 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. | ||
| 004152 | B6.129-Ctnnb1tm2Kem/KnwJ | Repository- Live |
| These mice possess loxP sites located in introns 1 and 6 of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to a strain expressing Cre recombinase in chrondocytes (see Stock No. 003554 for example), this mutant mouse strain may be useful in studies of chrondocyte differentiation. When bred to a strain expressing Cre recombinase in heart(see Stock No. 005650 or 005657 for example), this mutant mouse strain may be useful in studies of cardiovascular disease. When bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 007807 for example), this mutant mouse strain may be useful in ..... | ||
| 007708 | B6.129-Gt(ROSA)26Sortm1(HD*103Q)Xwy/J | Repository- Live |
| Mice heterozygous for the RosaHD mutant allele are viable and fertile. These mice have the neuropathogenic polyQ-mutant variant of the human Huntingtin protein (mhtt-exon1; 103Q) inserted into the Gt(ROSA)26Sor locus. Expression of mhtt-exon1 is blocked by an upstream loxP-flanked transcriptional STOP sequence. When bred to mice with a Cre recombinase gene under the control of a promoter of interest, the STOP sequence is deleted in the tissue of interest, and mhtt-exon1 expression is observed. As these RosaHD mutant mice allow cre-conditional expression of the neuropathogenic mhtt-exon1 protein, they may be useful in studying Huntington's disease (HD) or other polyQ disorders. Of note, sequencing of the polyQ region (using mice from the 11th backcross) indicate the actual number of repeats to be 98. For example, when bred to strains expressing cre in brain tissues (such as Nestin-Cre (see Stock No. 003771 ..... | ||
| 008463 | B6.129-Gt(ROSA)26Sortm1(cre/ESR1)Tyj/J | Repository- Live |
| A conditional Cre-ERT2 (Cre recombinase - estrogen receptor T2) cassette was introduced to the gene. The ERT2 moiety retains the Cre recombinase in the cytoplasm until tamoxifen administration releases this inhibition, thus permitting the recombination of genomic loxP sites. Efficient tamoxifen-induced Cre-mediated recombination throughout the body has been demonstrated through crosses with a Cre-responsive beta galactosidase reporter strain. This strain enables temporal control of floxed gene expression in vivo and is reportedly more sensitive to tamoxifen than Stock No. 004847. Homozygotes are viable and fertile. | ||
| 006075 | B6.129-Gt(ROSA)26Sortm3Luo/J | Repository- Live |
| MADM-GR mice are viable with no gross behavioral or observable abnormalities. Homozygous mice have low fertility, while heterozygous mice have no reported fertility defects. These mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to mice harboring a reciprocal mutation at the same locus (see Stock No. 006067 or Stock No. 006080, MADM-RG (Dsred2/EGFP)). The resulting offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous") and must next be bred to a Cre-expressing strain for fluorescent protein expression. Prior to Cre-recombination, double mutant mice do not have colored cells: the chimeric genes do not produce functional proteins because their coding sequences are interrupted by the beta-actin intron in different reading frames. After DNA replication (G2 phase) in double mutant mice, Cre-recom ..... For more information please see the full phenotype on the strain data sheet | ||
| 007561 | B6.129-Hif1atm3Rsjo/J | Repository- Live |
| These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s).
For example, when crossed to a strain expressing Cre recombinase in skeletal and cardiac muscle (see Stock No. 006475), this mutant mouse strain may be useful in studies of the metabolic control of muscle function. When bred to a strain with the targeted null allele in von Hippel-Lindau syndrome homolog, Vhlh (Stock No. 004081) and a strain expressing Cre recombinase in liver (Stock No. 003574), this mutant mouse strain may be use ..... | ||
| 008320 | B6.129-Leprtm2(cre)Rck/J | Repository- Live |
| Mice hemizygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Cre activity is demonstrable in the hypothalmus (arcuate, dorsomedial (DMH), lateral (LH), and ventromedial (VMH) nuclei), limbic and cortical brain regions (basolateral amygdaloid nucleus (BLA), piriform cortex (Pir), and lateral entorhinal cortex (LEnt)), and retrosplenial cortex. When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked genome in tissues that normally express the gene. This strain has been used in virus-assisted mapping of neural inputs and may be useful in studies of neural features of feeding behaviors. | ||
| 004584 | B6.129-Ppargtm2Rev/J | Repository- Live |
| These mice possess loxP sites on either side of exons 1 and 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to a strain expressing Cre recombinase in adipose tissue (see Stock No. 005069 for example), this mutant mouse strain may be useful in studies of insulin resistance. | ||
| 009666 | B6.129-Ppargc1atm2Brsp/J | Repository- Live |
| These mice possess loxP sites on either side of exons 3-5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3-5 deleted in the cre-expressing tissue(s). When bred to a strain expressing Cre recombinase specifically in the liver (see Stock No. 003574 for example), this mutant mouse strain may be useful in studies of hepatic heme biosynthesis and porphyrias. When bred to a strain expressing Cre recombinase specifically in skeletal muscle, this mutant mouse strain may be useful in studies of neuromuscular junction physiology and muscular dystrophy. | ||
| 008100 | B6.129-Prdm1tm1Clme/J | Repository- Live |
| These mice possess loxP sites in introns flanking exons 6 to 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 6 to 8 deleted in the cre-expressing tissue(s). When bred to a strain expressing Cre recombinase during B-lymphocyte development and differentiation (see Stock No. 004126 for example), this mutant mouse strain may be useful in studies of humoral immune response. | ||
| 006146 | B6.129-Smn1tm1Jme/J | Repository- Live |
| Mice homozygous for this SMNF7 floxed allele are viable and fertile and do not display any gross physical or behavioral abnormalities. Mutant mice exhibit no transcript splicing defects. Cre-mediated recombination of the loxP-flanked sequences results in deletion of exon 7 of the targeted gene. As mutations of this exon are implicated in 95% of all human spinal muscular atrophy (SMA), these mice may be useful in studying SMA or other neuromuscular degenerative diseases.
When crossed to a strain expressing Cre recombinase in neurons (see Stock No. 005938, Stock No. 006297, and Stock No. 006663), this mutant mouse strain may be useful as a model of SMA. When crossed to a strain expressing Cre recombinase in striated muscle fibers (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 006900 | B6.129-St3gal4tm1.1Jxm/J | Repository- Live |
| Mice homozygous for this targeted mutation are viable, fertile, and normal in size. They develop a bleeding disorder associated with an autosomal dominant reduction in plasma von Willebrand factor (VWF) and an autosomal recessive thrombocytopenia. The formation of selectin ligands on circulating neutrophils is also substantially reduced. This mutant mouse strain may be useful in studies of leukocyte trafficking and coagulation disorders. | ||
| 004146 | B6.129-Tg(Pcp2-cre)2Mpin/J | Repository- Live |
| These transgenic mice express a cre gene inserted into exon 4 of a Pcp2 gene. Mice homozygous for the insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity is observed in most Purkinje cells and some retinal bipolar neurons. Small amounts of activity are observed in an unidentified population of cells of the central nervous system tissue. Recombination is first observed around postnatal day 6 and is fully established 2 to 3 weeks after birth.
View cre expression characterization. | ||
| 006785 | B6.129P2(C)-Cd19tm1(cre)Cgn/J | Repository- Live |
| Homozygous mutant mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Homozygotes have a deficiency in the B-1 subset of B-lymphocytes along with a concomitant reduction in serum IgM. Their ability to respond to T-cell-dependent antigens is severely impaired, and they fail to form splenic germinal centers. In addition to disrupting the targeted gene, the targeting construct also introduced a cre cassette into exon 2 of the targeted gene, effectively placing cre expression under the control of the endogenous promoter. The Cd19 promoter specifically directs cre expression at the earliest stages and throughout B-lymphocyte development and differentiation. Although homozygous mutant mice are Cd19-deficient, heterozygous mice are phenotypically normal, and can be used for specific deletion of loxP-flanked (floxed) targets in B-lymphocytes.
In an attempt to offer alleles on well-characte ..... | ||
| 006084 | B6.129P2(Cg)-Foxg1tm1(cre)Skm/J | Repository- Live |
| This strain expresses Cre recombinase from the endogenous Foxg1 locus. Forkhead box G1 is required for telencephalon development and is expressed specifically in the telencephalon and discrete head structures. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in the telencephalon, anterior optic vesicle (developing lens and retina), otic vesicle, facial and head ectoderm, olfactory epithelium, mid-hindbrain junction and pharyngeal pouches. Mice that are homozygous for the targeted mutation die perinatally. Heterozygous mutant mice are viable, fertile, normal in size. On the C57BL/6 background, forebrain volume in heterozygotes is substantially reduced especially in the cerebral cortex (40.7%), striatum (29.7%), and hippocampus (18.6%). In the adult, the thalamus is reduced in volume by 21.6%. This mutant mouse strain represents a model that ma ..... For more information please see the full phenotype on the strain data sheet | ||
| 008765 | B6.129P2-Cbfbtm1Itan/J | Repository- Live |
| These mice possess loxP sites on either side of exon 5 in the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.
When bred to CD4-cre mice, homozyogous animals have reduced peripheral T cell numbers, abnormal CD4/CD8 expression, and altered response to antigen receptor stimulation. In addition, these mice show autoimmune colitis and asthma-like syndrome. | ||
| 008513 | B6.129P2-Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/J | Repository- Live |
| A loxP-flanked neomycin cassette blocks expression of the rat Trpv1 (transient receptor potential cation channel, subfamily V, member 1) gene driven by the Gt(ROSA)26Sor gene in this targeted mutation/knock-in strain. Upon crossing to a tissue-specific Cre-expressing strain, TRPV1 and enhanced cyan fluorescent protein (ECFP) is expressed from the ROSA locus. Cells expressing TRPV1 are sensitive to capsaicin and similar chemical agonists. Treatment of mice or cells that have undergone Cre excision to remove the neomycin cassette can induce strong inward currents, trigger action potentials and activate stereotyped behaviors, allowing cell-type specific chemical genetic control of neuronal activity in vitro and in vivo. Mice that are homozygous for the floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
When bred to a strain with widespread expression of Cre recombinase (see Stock No. > ..... | ||
| 008710 | B6.129P2-Hprttm10(Ple162-EGFP/cre)Ems/J | Repository- Live |
| These Ple162-EGFPCre;mEMS954 mice have the Ple162-EGFPCre transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the human paired-like homeodomain 3 (PITX3) gene directing expression of an enhanced green fluorescent protein/Cre recombinase fusion protein (EGFPCre). These Ple162-EGFPCre;mEMS954 mice may be useful in studying PITX3-expressing cells in the brain and diseases affecting the brain, including mesocorticolimbic dopamine system function in the drug and natural reward circuitry of the brain, cognition, motivation, drug addiction, and psychiatric disorders.
For Ple162-EGFPCre;mEMS954 expression information, see The Pleiades Promoter Project website (Ple162 Promoter (pEMS1148)). The donati ..... | ||
| 008877 | B6.129P2-Hprttm12(Ple177-EGFP/cre)Ems/J | Repository- Live |
| These Ple177-EGFPCre;mEMS762 mice have the Ple177-EGFPCre transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the human regulator of G-protein signaling 16 (RGS16) gene directing expression of an enhanced green fluorescent protein/Cre recombinase fusion protein (EGFPCre) to hippocampus, cortex, and cerebellum. These Ple177-EGFPCre;mEMS762 mice may be useful in studying RGS16-expressing cells in the brain and diseases affecting the brain.
For Ple177-EGFPCre;mEMS762 expression information, see The Pleiades Promoter Project website (Ple177 Promoter (pEMS1089)). The donating investigator reports: | ||
| 008709 | B6.129P2-Hprttm9(Ple178-EGFP/cre)Ems/J | Repository- Live |
| These Ple178-EGFPCre;mEMS756 mice have the Ple178-EGFPCre transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the human regulator of G-protein signaling 16 (RGS16) gene directing expression of an enhanced green fluorescent protein/Cre recombinase fusion protein (EGFPCre). These Ple178-EGFPCre;mEMS756 mice may be useful in studying RGS16-expressing cells in the brain and diseases affecting the brain.
For Ple178-EGFPCre;mEMS756 expression information, see The Pleiades Promoter Project website (Ple178 Promoter (pEMS1090)). The donating investigator reports: | ||
| 008875 | B6.129P2-Lgr5tm1(cre/ESR1)Cle/J | Repository- Live |
| While homozygous mice are not viable, heterozygous Lgr5-EGFP-IRES-CreERT2 mice are viable and fertile; harboring a Lgr5-EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 (Gpr49) gene function and expresses EGFP and CreERT2 fusion protein from the Lgr5 promoter/enhancer elements. EGFP fluorescence is observed in crypt base columnar cells in small intestine (aka stem cells of the small intestine) and colon. Cre-ERT2 fusion gene activity is inducible; observed in the same cells only following taxomifen administration. EGFP or inducible CreERT2 expression may also be observed in other Lgr5-expressing cell types (including pre-malignant mouse adenomas, colon cancer cells, epithelial stem cells of the stomach gland, basal epithelial layer stem cells of the mammary glands, and hair follicle stem cells).
The donating investigator reports variegated expression of the Lgr5-EGFP-IRES-CreERT2 transgene in the small intestine and colon (something which may h ..... | ||
| 004781 | B6.129P2-Lyz2tm1(cre)Ifo/J | Repository- Live |
| This strain expresses Cre recombinase from the endogenous Lyzs locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the myeloid cell lineage, including monocytes, mature macrophages, and granulocytes. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain represents an effective tool for generating myeloid cell-specific targeted mutants. | ||
| 007177 | B6.129P2-Mecp2tm1Bird/J | Repository- Live |
| These mice possess two functional loxP sites flanking exons 3-4 of the targeted gene on the X chromosome (the donating investigator reports that the middle loxP site is non-functional). Homozygous females and hemizygous males are viable and fertile. Northern blot analysis showed the expected mature transcript from the Mecp2lox locus as well as a transcript in which the beta-globin intron was unspliced. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3-4 deleted in the cre-expressing tissue(s). Mice with this X-linked floxed mutation may be useful in neurological and developmental studies of Rett syndrome. For example, when crossed to a strain expressing Cre recombinase in nervous tissue (see Stock No. 003771), this mutant mouse strain develops a neurological phenotype that mimics Rett syndrome. In an attempt to offer alleles on well ..... | ||
| 006849 | B6.129P2-Mecp2tm2Bird/J | Repository- Live |
| These mice possess a loxP-flanked STOP cassette in intron 2 of the targeted gene on the X chromosome. Western blot and hybridization analysis confirm the absence of wildtype protein from the targeted allele (although the donating investigator reports that the targeted allele produces a "read-through" transcript which does not give rise to detectable levels of protein but makes it difficult to discriminate between the "flox-stopped" and reactivated alleles by RT-PCR). Hemizygous (Mecp2lox-Stop/y) males do not breed and develop Rett syndrome symptoms (reduced mobility, hindlimb clasping) at approximately 6 weeks of age, with death occurring at approximately 11 weeks of age. Heterozygous females are fertile until developing Rett syndrome characteristics at 4-12 months of age. This Rett syndrome-like phenotype is similar to that observed for the traditional knock-out allele (see Stock No. 003890). Cre recombin ..... For more information please see the full phenotype on the strain data sheet | ||
| 008888 | B6.129P2-Myd88tm1Defr/J | Repository- Live |
| These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s).
When bred to a strain with inducible Cre recombinase expression in dendritic cells (see Stock No. 008068 for example), this mutant mouse strain may be useful in studies of Toll-like receptor signaling during immune responses. When bred to a strain with Cre recombinase expression in hematopoietic cells (see Stock No. 008610 for example), this mutant mouse strain may be useful in studies of Toll-like receptor signaling and natural killer cells. | ||
| 009154 | B6.129P2-Polbtm1Rsky/J | Repository- Live |
| These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissue(s). This mutant mouse strain may be useful in generating conditional mutations for studying DNA repair. | ||
| 007685 | B6.129P2-Psen1tm1Vln/J | Repository- Live |
| These mice possess loxP sites on either side of exon 7 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these "floxed" mice are bred to mice that express Cre recombinase, resulting offspring can have one of three resulting genotypes (only exon 7 deleted, only the neo selection cassette deleted, or both exon 7 and the neo selection cassette deleted) in the cre-expressing tissue(s). These PS1-floxed mice may be useful in generating conditional knockouts of Presenilin 1 for studying Alzheimer's Disease.
For example, when crossed to a strain expressing Cre recombinase in postnatal neurons (see Stock No. 006143), this mutant mouse strain may be useful in studies of amyloid plaque formation. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are ..... | ||
| 008336 | B6.129P2-Ptpn6tm1Rsky/J | Repository- Live |
| Mice homozygous for the Ptpn6f allele are viable and fertile, with loxP sites flanking exon 1(II) through most of exon 9 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in cre-expressing tissue(s). These Ptpn6f mice may be useful in generating conditional mutations for studying the role of Ptpn6 (Shp1) in inflammation and immunology research. For example, when bred to a strain with inducible Cre recombinase expression in liver and lymphocytes (see Stock No. 003556), this mutant mouse strain may be useful in studying the motheaten (me) phenotype; characterized by widespread inflammation and autoimmunity.
When bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 004126, Stock No. > ..... | ||
| 008773 | B6.129P2-Runx3tm1Itan/J | Repository- Live |
| Exon 4 of these targeted mutant mice is flanked by loxP sites. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. When crossed to either a CD4-cre or Lck-cre mouse strain, the numbers of CD8+ mature thymocytes and CD8+ T cells in spleen or lymph nodes are reduced and show defective responses to antigen receptor stimulation. CD8+ T cells express CD4 and the ectopic CD4 expression is enhanced when the floxed region is excised. | ||
| 008462 | B6.129P2-Trp53tm1Brn/J | Repository- Live |
| Exons 2-10 are flanked by loxP sites in this conditional targeted mutation. Mice homozygous for the floxed allele do not show any increase in disease incidence for at least a year. When bred to mice with a cre recombinase gene under the control of a promoter of interest, expression is deleted in the tissue of interest. For example, when crossed to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771), this mutant mouse strain may be useful in studies of medulloblastoma formation. When crossed to a strain expressing Cre recombinase in the central nervous system (see Stock No. 004600), this mutant mouse strain may be useful in studies of astrocytoma formation. | ||
| 005623 | B6.129S-Shhtm2(cre/ESR1)Cjt/J | Repository- Live |
| This strain expresses a fusion product involving Cre recombinase and a mutant form of the human estrogen receptor ligand binding domain from the endogenous Shh locus. The mutant human estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/ESR1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. Tamoxifen administration induces Cre recombinase expression in all cells that express the endogenous gene resulting in the deletion of the first 35 base pairs following the ATG. Homozygous mice are not viable or fertile. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of limb patterning and development. | ||
| 009380 | B6.129S1-Irf4tm1Rdf/J | Repository- Live |
| These mice possess loxP sites on either side of exons 1 and 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 1 and 2 deleted and GFP expression in the cre-expressing tissue(s). When crossed to mice that express Flp recombinase, the entire targeting construct, including exons 1 and 2, are deleted in the FLP expressing tissues. When bred to a strain expressing Cre recombinase in B lymphocytes (see Stock No. 006785 for example), this mutant mouse strain may be useful in studies of plasma cell development and immunoglobulin class switch recombination.
When bred to a strain expressing Cre recombinase in T regulatory cells, this mutant mouse strain may be useful in studies of autoimmune ..... | ||
| 006600 | B6.129S1-Mnx1tm4(cre)Tmj/J | Repository- Live |
| Mice heterozygous for this HB9cre targeted mutation are viable and fertile, with cre expression replacing HB9 (Hlxb9 or Mnx1) expression. Under control of the endogenous upstream elements, cre expression is directed to motor neurons. In heterozygotes, cre expression coincides with HB9 expression. Homozygous HB9cre mice die at or soon after birth, with expression of Cre recombinase likewise directed to motor neurons but no expression of endogenous HB9. When these HB9cre mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination in the resulting offspring leads to deletion of the flanked sequences in Mnx1/HB9 expressing cells; making them useful in neurodevelopmental studies of homeobox genes, motor neuron function and differentiation, and the central nervous system. | ||
| 005628 | B6.129S2-Emx1tm1(cre)Krj/J | Repository- Live |
| Mice homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain expresses Cre recombinase from the endogenous Emx1 locus. Western blot analysis of cortical brain tissue does not detect reduced endogenous gene product (protein). When crossed with a strain containing a loxP-site flanked sequence, Cre-mediated recombination results in tissue-specific deletion of the flanked sequence. Recombination occurs in approximately 88% of the neurons of the neocortex and hippocampus, and in the glial cells of the pallium, mimicking the pattern of expression of the endogenous gene. Further, the donating investigator reports that Cre recombinase is also expressed in a subset of male germline cells, thus some offspring from a cre; floxed male will have the floxed allele recombined in all cells. This mutant mouse strain represents a model that may be useful in studies of forebrain development and f ..... For more information please see the full phenotype on the strain data sheet | ||
| 007668 | B6.129S4(Cg)-Arntltm1Weit/J | Repository- Live |
| Mice homozygous for this conditional (floxed) allele possess loxP sites flanking exon 8 of the targeted gene and are viable and fertile, with circadian behavioral rhythms indistinguishable from wildtype littermates. When bred to mice that express Cre recombinase, the resulting offspring will have the exon encoding the ARNTL (BMAL1) basic helix-loop-helix (bHLH) domain deleted in the cre-expressing tissue(s). These Bmal1-floxed mutant mice may be useful in generating conditional mutations (whole-mouse or tissue-specific) to study the role of circadian clock/circadian rhythm in physiological and behavioral regulation.
For example, when crossed to a strain expressing Cre recombinase in the retina (see Stock No. 005105), this mutant mouse strain may be useful in studies of the circadian clock of the retina. | ||
| 005246 | B6.129S4-Grin1tm2Stl/J | Repository- Live |
| These mice possess loxP sites flanking approximately 12kb of sequence of the targeted gene that encodes the entire transmembrane domain and C-terminal region. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to a strain expressing Cre recombinase in the CA3 region of the hippocampus (see Stock No. 006474 for example), this mutant mouse strain may be useful in studies of associative memory recall. When bred to a strain expressing Cre recombinase in the CA1 region of the hippocampus (see Stock No. 005359 for example), this mutant mouse strain may be useful in studies of nonspacial memory. | ||
| 009044 | B6.129S4-Gt(ROSA)26Sortm2Tyj/J | Repository- Live |
| Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In the absence of Cre, there is low level expression of Luciferase-SIY H2b antigen expression. After Cre activity, the recombined locus has high expression of Luciferase-SIY fusion protein. This mutant mouse strain may be useful in studies of immune response to a self-antigen or over-expressed tumor-associated antigen when used in combination with tumor-prone models. This mouse is also useful as a reporter for Cre activity. | ||
| 008179 | B6.129S4-Krastm4Tyj/J | Repository- Live |
| This strain carries a point mutation (G12D) whose expression is blocked by the presence of a loxP-flanked stop codon. Homozygotes die in utero. Cre-mediated recombination can excise the stop codon and permit the oncogenic protein to be expressed. Intranasal infection with an adenovirus encoding Cre results in a very high frequency of lung tumors and permits controlled timing of tumor initiation and tumor multiplicity. This strain may be useful in studies of cancer and development. When bred to a strain expressing Cre recombinase under the control of a tetracycline-responsive promoter element and a strain expressing a tetracycline-controlled activator protein in lung epithelial cells (see Stock No. 006234 and 006235 respectively), this mutant mouse strain may be useful in studies of lung development.
When bred to a strain expressing Cre recombinase in the male g ..... | ||
| 006503 | B6.129S4-Lpltm1Ijg/J | Repository- Live |
| These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. These mice may be useful for cardiovascular studies (such as lipid metabolism and fat storage) and obesity research. | ||
| 003755 | B6.129S4-Meox2tm1(cre)Sor/J | Repository- Live |
| This strain expresses Cre recombinase under the control of the endogenous Meox2 promoter. Expression of Cre recombinase is observed in epiblast-derived tissues as early as embryonic day 5. The insertion creates a null allele for the Meox2 gene. Homozygous mice are viable on this background but exhibit an overall reduction in muscle mass and the absence of specific muscles resulting in abnormal limb posture and reduced motility. This phenotype is variable. As many as 80% of homozygotes are severely affected, fail to thrive and die before weaning. Some homozygotes (10%) exhibit clefting of the secondary palate. These mice can be utilized as a deleter strain for loxP flanked DNA and provide an alternative to tetraploid embryo analysis. | ||
| 007893 | B6.129S4-Myf5tm3(cre)Sor/J | Repository- Live |
| This strain expresses Cre recombinase from the endogenous Myf5 locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs skeletal muscle and the dermis, and in several ectopic locations. Homozygotes for this allele have a perinatal lethal phenotype and die at birth. Homozygotes display abnormal rib development and some fusions of the cervical or thoracic vertebrae. This mutant mouse strain represents a model that may be useful in studies of skeletal development.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as p ..... | ||
| 006440 | B6.129S4-Ptentm1Hwu/J | Repository- Live |
| These mice possess loxP sites flanking exon 5 of the targeted gene. Mice homozygous for the"floxed" allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description as published results become available. | ||
| 006447 | B6.129S6(CBA)-Cebpatm1Dgt/J | Repository- Live |
| Mice carrying this C/EBPalpha "floxed" allele (C/EBPalphaF) are viable and fertile. The floxed allele functions similarly to the wildtype allele. In mice homozygous for C/EBPalphaF and expressing an interferon-inducible Cre recombinase (introduced by breeding to a cre-expressing strain; see Stock No. 003556), C/EBPalpha activity is disrupted, leading to defective myeloid cell development, increased hematopoietic stem cell repopulating activity, and significantly increased myeloblast population in the bone marrow compartment. In combination with an appropriate Cre transgenic strain, these mutant mice may be useful in studies of hematopoietic cell (e.g. myeloid and basophil progenitor cell) development and function, cancer (e.g. acute myeloid leukemia), and alveolar cell differentiation. | ||
| 007611 | B6.129S6(SJL)-Cdh2tm1Glr/J | Repository- Live |
| These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissue(s). When bred to a strain with inducible Cre recombinase expression in cardiac cells (see Stock No. 005657 for example), this mutant mouse strain may be useful in studies of myocardium physiology. | ||
| 006658 | B6.129S6-Srftm1Rmn/J | Repository- Live |
| These mice possess loxP sites that flank promoter and exon 1 sequences. Mice that are homozygous for this allele are viable and fertile. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Excision of the floxed fragment results in the removal of key regulatory elements and many of the DNA-binding and dimerization residues of the gene. When bred to a strain expressing Cre recombinase under the control of mouse transgelin promoter (see Stock No. 004746 for example), this mutant mouse strain may be useful in studies of cardiovascular disease. | ||
| 006878 | B6.129S6-Taglntm2(cre)Yec/J | Repository- Live |
| Mice homozygous for this SM22alpha-CreKI allele are viable and fertile. These mice have a Cre-recombinase gene inserted into the endogenous transgelin (SM22alpha) locus. The donating investigator reports that this mutation results in a loss of function of the targeted gene. Cre recombinase activity is shown in adult smooth muscle cells (such as arteries, veins, and visceral organs) and cardiac myocytes, but activity is not observed in the same embryonic tissues. It has been the experience of The Jackson Laboratory that optimal breeding is achieved by mating heterozygous females to homozygous males as female mortality post gestation has been noted in our colony. These SM22alpha-CreKI mice may be useful for Cre-lox technology applications in studying smooth muscle and cardiac gene function, as well as cardiovascular disease. | ||
| 008681 | B6.129S7-Atoh1tm3Hzo/J | Repository- Live |
| These mice possess loxP sites on either side of the coding region of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in further elucidating the role of this gene in cell fate commitment. | ||
| 008039 | B6.129S7-Gja1tm1Dlg/J | Repository- Live |
| Mice homozygous for this Cx43flox conditional allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. Presence of the loxP sites has no reported affect on expression of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have exon 2 deleted in the cre-expressing tissue(s). These Cx43flox mutant mice may be useful in generating conditional mutations for studying the role of connexin and gap junctions in various tissues and systems, including the cardiovascular system. | ||
| 006148 | B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J | Repository- Live |
| Mice that are homozygous for the mutant allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice contain an Enhanced Yellow Fluorescent Protein gene (EYFP, Clonetech) inserted into the Gt(ROSA)26Sor locus. Expression of EYFP is blocked by an upstream loxP-flanked STOP sequence. When bred to mice with a cre recombinase gene under the control of a promoter of interest, the STOP sequence of the targeted gene is deleted in the tissue of interest, and EYFP expression is observed. These mutant mice may be useful in monitoring the Cre expression in living tissues, and tracing the lineage of such cells in embryos, young, and adult mice at desired time points.
Mutant mice maintained on a genetic background containing a contribution from C57BL/6J have the potential to harbor a loss-of-function mutation in the nicotinamide (NAD) nucleotide transhydrogenase gene (Nnt, Chromosome 13). This ..... | ||
| 007181 | B6.129X1-Notch1tm2Rko/GridJ | Repository- Live |
| Mice homozygous for this "floxed" Notch1 allele (fN1) are viable and fertile. These mice possess loxP sites on either side of exon 1 of the targeted gene. When bred to mice with a Cre recombinase gene, exon 1 of the targeted gene is deleted in the cre expressing tissue(s). These conditional knockout mice may be useful in generating tissue-specific mutants for studying the development of a wide range of tissues: for example, when crossed to a strain expressing Cre recombinase primarily in the nervous system (see Stock No. 003771), this mutant strain may be useful in studies of apoptosis in neural development.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modi ..... | ||
| 008712 | B6.129X1-Twist2tm1.1(cre)Dor/J | Repository- Live |
| Dermo1-cre (Twist2-cre) mutant mice harbor a Cre recombinase "knock-in" allele that also abolishes endogenous Twist2 gene function. Heterozygotes are viable and fertile, while homozygotes (twist-2-/-) die a few days after birth. Under control of the upstream promoter/enhancer elements, cre expression is observed in a pattern consistent with the wildtype gene; Cre recombinase activity is reported in mesoderm as early as embryonic day 9.5, in mesodermal tissues such as branchial arches and somites, and in condensed mesenchyme-derived chondrocytes and osteoblasts. When heterozygotes are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequences in Dermo1-expressing tissues of the offspring. Homozygous mice exhibit elevated expression of proinflammatory cytokines resulting in perinatal death from cachexia (wasting), as well as progressive growth retardation, impaired movement, th ..... For more information please see the full phenotype on the strain data sheet | ||
| 006054 | B6.C-Tg(CMV-cre)1Cgn/J | Repository- Live |
| In this transgenic strain, deletion of loxP-flanked genes occurs in all tissues, including germ cells. The cre gene in this strain is under the transcriptional control of a human cytomegalovirus minimal promoter and is likely to be expressed before implantation during early embryogenesis. It also appears that the cre gene is X-linked since transgene transmission through males is restricted to female offspring. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available. | ||
| 009642 | B6.Cg(129)-Tg(Gh1-cre)1Sac/J | Repository- Live |
| These transgenic mice express Cre recombinase under the control of the human Growth Hormone (GH1) Locus Control Region and the rat growth hormone 1, (Gh1) promoter. When crossed with a reporter strain, Cre recombinase expression is seen during development and in the adult in pituitary somatotropes. Weaker expression is detected in lactotropes. Cre expression is detected in the forelimb skin of embryos aged embryonic day 16.5-17.5, and weaker expression in adult kidney, ovary, and testis. Southern blot analysis reveals approximately 50 copies or the transgene segregated to one locus. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study developmentally critical gene function in the anterior pi ..... For more information please see the full phenotype on the strain data sheet | ||
| 006230 | B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J | Repository- Live |
| Mice homozygous for this C/EBPalpha "floxed" allele (C/EBPalphaF) and hemizygous for the Mx1-cre transgene are viable and fertile, and exhibit no abnormalities in the hematopoietic system. In the absence of cre expression, the C/EBPalphaF allele functions similarly to the wildtype allele. Mx1-Cre transgene expression can be induced by administration of either interferon (alpha or beta) or synthetic double-stranded RNA (such as poly I:C), leading to deletion of the "floxed" gene. Following 3-4.5 weeks of poly I:C treatment, deletion efficiency is greater than 95% in hematopoietic tissues, and C/EBPalpha protein is undetectable in bone marrow. These poly I:C-treated, mice have defective myeloid cell development, increased hematopoietic stem cell repopulating activity, and a significantly increased myeloblast population in the bone marrow compartment. These mutant mice may be useful in studies of hematopoietic cell (e.g. myeloid and basophil progenitor cell) d ..... For more information please see the full phenotype on the strain data sheet | ||
| 006366 | B6.Cg-Dicer1tm1Bdh/J | Repository- Live |
| These mice contain loxP sites on either side of exon 23. Homozygous mice are viable and fertile with no gross phenotypic or behavioral abnormalities. Expression of the targeted allele is indistinguishable from wild-type despite the frt-flanked neomycin cassette. Cre-mediated recombination (resulting in deletion of exon 23) in the germline leads to developmental arrest at embryonic day 7.5 (E7.5). Tissue specific deletion has been shown to result in loss of microRNA (miRNA) processing. Mutant mice can be used to generate cell/tissue-specific deletions of the endogenous gene for applications in embryonic development, translation, protein processing and miRNA/siRNA regulation of gene expression. For example, when crossed to a strain expressing Cre recombinase in mesenchyme (see Stock No. 005584), this mutant mouse strain may be useful in studies of limb morphogenesis. In an attempt to offer alleles on well-charac ..... | ||
| 005670 | B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J | Repository- Live |
| Homozygous mutant mice are viable, fertile, normal in size and do not display any behavioral abnormalities. When these gene targeted mice are bred to transgenic strains expressing Cre recombinase, functional rtTA and EGFP activity is observed in the double mutant offspring in the tissues that express cre. These double mutant mice may be bred to transgenic strains carrying genes of interest under the regulation of tetracycline responsive elements (TRE; tetO) to generate triple mutant mice in which the tissue specificity of the cre-transgenic line and doxycycline inducibility of the rtTA/TRE-controlled transgenes can be combined to regulate expression of the target gene. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify th ..... | ||
| 007914 | B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | Repository- Live |
| Ai14 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE::deltaNeo conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. Ai14 mice are not expected to express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre is expected to be detectable by fluorescence and mRNA (in situ hybridization). These Ai14 mice are useful as a Cre reporter strain; expressing the red fluorescent protein variant, tdTomato, following Cre-mediated recombination.
Of not ..... | ||
| 007920 | B6.Cg-Gt(ROSA)26Sortm2(CAG-EYFP)Hze/J | Repository- Live |
| Ai2 mice hemizygous for this Rosa-CAG-LSL-EYFP conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream enhanced yellow fluorescent protein (EYFP). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of EYFP. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, EYFP expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai2 mice do not express EYFP prior to introduction of Cre recombinase and EYFP fluorescence following exposure to cre is weak but easily detected by mRNA (in situ hybridization) and antibody staining (immunohistochemistry). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-mediated ..... For more information please see the full phenotype on the strain data sheet | ||
| 007903 | B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J | Repository- Live |
| Ai3 mice hemizygous for this Rosa-CAG-LSL-EYFP-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream enhanced yellow fluorescent protein (EYFP). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of EYFP. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, EYFP expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai3 mice do not express EYFP prior to introduction of Cre recombinase and EYFP expression following exposure to cre can be detected by fluorescence, mRNA (in situ hybridization) and antibody staining (immunohistochemistry). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-medi ..... For more information please see the full phenotype on the strain data sheet | ||
| 006492 | B6.Cg-Pdgfratm8Sor/EiJ | Repository- Live |
| These mice possess loxP sites on either side of exon 1 and exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.
For example, when bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 007807 for example), this mutant mouse strain may be useful in studies of cardiac neural crest cell migration. | ||
| 005622 | B6.Cg-Shhtm1(EGFP/cre)Cjt/J | Repository- Live |
| This strain expresses a fusion product involving Enhanced Green Fluorescent Protein (EGFP) and Cre recombinase from the endogenous Shh locus. EGFP and cre expression are consistent with the endogenous gene. Fluorescence is detected in the distal posterior region of the limb buds of embryos aged embryonic day 10 to 12 and colocalizes with the endogenous gene product (mRNA).
The donating investigator reports that it is not uncommon for a mosaic expression pattern to be exhibited when the allele is inherited through the female germline. It is recommended that this allele be passed through the male germline when conducting experiments involving cre-induced recombination. Mice homozygous for the mutation develop a limited limb skeleton and lack digit 2. Homozygous mice are not viable or fertile. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
This mutant mouse strain may be useful in studie ..... For more information please see the full phenotype on the strain data sheet | ||
| 006149 | B6.Cg-Tg(ACTA1-cre)79Jme/J | Repository- Live |
| Mice hemizygous for this HSA-Cre79 transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These HSA-Cre79 transgenic mice have the cre recombinase gene driven by the human alpha-skeletal actin (HSA or ACTA1) promoter. Cre activity is restricted to adult striated muscle fibers and embryonic striated muscle cells of the somites and heart. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in striated muscle-specific deletion of the flanked genome. Specifically, these HSA-Cre79 (or ACTA1-Cre) transgenic mice were originally used to breed with mice heterozygous for a deletion of exon 7 and a loxP-flanked exon 7 mutation on homologous chromosomes of the Smn1 gene (see Stock No. 006138 or Stock No. 006146). The resulting offspring ( ..... For more information please see the full phenotype on the strain data sheet | ||
| 003574 | B6.Cg-Tg(Alb-cre)21Mgn/J | Repository- Live |
| This strain may be maintained as a hemizygote or homozygote and lacks any phenotype related to the transgenic allele. It has been well characterized and shown to be very efficient for performing liver-specific gene knockouts using Cre/loxP system. This line has been shown to be nearly 100% efficient in achieving liver-specific recombination when crossed with at least 5 different floxed alleles.
View cre expression characterization. | ||
| 006881 | B6.Cg-Tg(Aqp2-cre)1Dek/J | Repository- Live |
| Mice hemizygous for this AQP2-Cre transgene are viable and fertile. Transgenic cre activity, directed by the mouse aquaporin 2 promoter, is observed in kidney cells (collecting duct) and testes (sperm). When bred with mice containing a loxP-flanked sequence of interest, cre-mediated recombination will result in deletion of the flanked sequence. In such breedings, maternal inheritance of the transgene is recommended for kidney-specific recombinase activity as males express cre in sperm as well as kidney tissues. These AQP2-Cre mice may be used to generate conditional mutations in the renal collecting duct for studying nephrology, physiology, metabolism, or type II diabetes. In addition, cre expression in sperm may be useful in generating conditional mutations in multiple or all tissues in the resulting offspring. | ||
| 004682 | B6.Cg-Tg(CAG-cre/Esr1)5Amc/J | Repository- Live |
| These transgenic mice have a tamoxifen-inducible cre-mediated recombination system driven by the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice. Homozygous mice are not viable or fertile. Heterozyg ..... For more information please see the full phenotype on the strain data sheet | ||
| 008520 | B6.Cg-Tg(CD2-cre)4Kio/J | Repository- Live |
| Mice hemizygous for this hCD2-iCre transgene are viable and fertile, with the human CD2 promoter and locus control region (LCR) directing expression of an optimized variant of Cre recombinase (iCre) to T cells and B cells (all committed B cell and T cell progenitors). Using crosses to a reporter strain, variegated germ line (testis) and a small monocyte-enriched population expression is also reported. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These hCD2-iCre transgenic mice may be useful for generating conditional mutations in T cells and B cells. Of note, this hCD2-iCre strain (Stock No. 008520) allows reliable deletion/gene targeting to be focused to T cells and B cells, whereas the Vav-iCre strain (Stock No. 008610) allows targeting throughout the entire hematopoietic compartment. IMPOR ..... | ||
| 009350 | B6.Cg-Tg(CDX2-cre)101Erf/J | Repository- Live |
| Mice hemizygous for the CDX2P9.5-NLSCre transgene are viable and fertile, with a 9.5 kb human caudal type homeo box 2 (CDX2) promoter/enhancer sequence directing expression of a nuclear-localized Cre recombinase predominantly to colonic epithelium during late gestation and in adult tissues. Specifically, Cre recombinase expression is observed in epithelium from the distal ileum and cecum, and throughout the colon from the crypt base to the luminal surface. Cre recombinase expression is also observed throughout the caudal region of the embryo during early development. When these transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. | ||
| 005359 | B6.Cg-Tg(Camk2a-cre)T29-1Stl/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the Cre recombinase under the control of the mouse calcium/calmodulin-dependent protein kinase II alpha promoter. Cre recombinase expression is detected in the forebrain, specifically to the CA1 pyramidal cell layer in the hippocampus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination occurs in the pyramidal cell layer. | ||
| 006137 | B6.Cg-Tg(Cdh5-cre)7Mlia/J | Repository- Live |
| Hemizygotes are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. In the differentiated endothelium transgene expression is observed as early as E7.5 and progresses to almost full penetrance by E14.5. In adult mice, uniform cre expression is observed in the endothelium of developing and quiescent vessels of all organs examined, as well as within a subset of hematopoietic cells. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome. These mice may be useful in studies of the cardiovascular system, including angiogenesis, and endothelial and hematopoietic cell lineages. | ||
| 006368 | B6.Cg-Tg(Cr2-cre)3Cgn/J | Repository- Live |
| Mice homozygous for this "CD21-cre3a" transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse complement receptor 2 (CR2) promoter. Cre recombinase expression is detected specifically in mature transitional B cells. When CD21-cre3a mice were crossed to a lacZ-expressing Cre reporter strain, Cre recombinase activity is detected in 65-70% of mature B cells (as determined by FACS analysis of bone marrow cells). When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in mature B lymphocytes and follicular dendritic cells. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study B lymphocyte development. | ||
| 005069 | B6.Cg-Tg(Fabp4-cre)1Rev/J | Repository- Live |
| These transgenic mice express Cre recombinase under the control of the mouse Fabp4, fatty acid binding protein 4, adipocyte, promoter. Cre recombinase expression is detected in brown and white gonadal and subcutaneous adipose tissue. No expression is detected in skeletal muscle. When crossed with a strain containing a loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene specifically in adipose tissue. Mice that are homozygous for the targeted mutation are viable. This strain represents an effective tool for generating tissue-specific targeted mutants.
View cre expression characterization. | ||
| 003573 | B6.Cg-Tg(Ins2-cre)25Mgn/J | Repository- Live |
| This strain carries the "RIP-Cre" transgene construct (containing a 668 bp fragment of the rat insulin II promoter, Cre recombinase with a nuclear localization sequence, and a 2.1 kb fragment from the human growth hormone gene). Hemizygous mice carrying this transgene are phenotypically normal and overexpresss cre specifically in pancreatic beta cells. This transgene strain is used in combination with mice carrying floxed targeted mutations to create various pancreatic beta cell-specific gene knockouts using the "Cre-lox" system. Results from several different laboratories have shown that this transgenic strain is at least 85% efficient in achieving pancreatic beta cell-specific recombination. It should also be noted that the transgene in this line has been found to be expressed at a low level in the hypothalamus. In some cases this has resulted in a phenotype due to deletion of the floxed allele in this region of the brain. It has also been shown that these transgenic mice may ..... For more information please see the full phenotype on the strain data sheet | ||
| 008068 | B6.Cg-Tg(Itgax-cre)1-1Reiz/J | Repository- Live |
| Mice hemizygous for the Cd11c-cre transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse integrin alpha X (Cd11c) promoter. Cre-mediated recombination is detected in more than 95% of conventional CD11chigh dendritic cells both from lymphoid organs and from non-lymphoid tissues such as lung and epidermis, and in 50-80% of plasmacytoid dendritic cells. The dendritic cell compartment of transgenic mice is normal. Relatively low amounts of recombination are detected in lymphocytes (<10%), NK cells (12%), and myeloid cells (<1%). No increase of recombination frequency was observed in CD11clow- activated T cells. These Cd11c-cre transgenic mice (as well as CD11c-Cre-GFP transgenic mice (see Stock No. 007567)) are an effective tool for generating tissue-specific targeted mutants for > ..... For more information please see the full phenotype on the strain data sheet | ||
| 008781 | B6.Cg-Tg(Kap-cre)29066/2Sig/J | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase (iCre, improved cre) under the control of the mouse kidney androgen regulated protein (Kap). Cre recombinase expression is detected in the proximal tubule cells of the renal cortex in male mice. Female mice do not express the transgene unless treated with androgen (testosterone). When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the proximal tubule cells of the kidney. | ||
| 003802 | B6.Cg-Tg(Lck-cre)548Jxm/J | Repository- Live |
| Homozygous mice are viable and have no major defects. This strain expresses Cre recombinase in thymocytes. | ||
| 009643 | B6.Cg-Tg(Lhb-cre)1Sac/J | Repository- Live |
| These transgenic mice express Cre recombinase under the control of the bovine LHB, luteinizing hormone beta polypeptide promoter. Although the transgenic construct contains sequence encoding a fusion gene of EGFP and Cre recombinase, no EGFP fluorescence is detected. When crossed with a reporter strain, Cre recombinase expression is seen in mature pituitary gonadotropes, specifically in cells that produce luteinizing hormone beta. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study developmentally critical gene function in the pituitary. | ||
| 003556 | B6.Cg-Tg(Mx1-cre)1Cgn/J | Repository- Live |
| The Cre recombinase is under the control of the Mx1 promoter. This promoter is silent in healthy mice, but can be induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA (such as poly I:C). When combined with a mutant carrying a gene that has been flanked by loxP recognition sites, the expression of Cre recombinase causes the flanked gene to be removed. This provides researchers with the capability to induce the "knockout" at any time during development. There was ~1% background recombination seen in mice not treated with interferon. The percent deletion of the targeted gene varied depending on tissue type, presumably due to the amount of interferon-responsive cells present or to the availability of interferon in each organ.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an ..... | ||
| 007742 | B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J | Repository- Live |
| Mice hemizygous for the smMHC/Cre/eGFP transgene (smMHCCre/eGFP) are viable and fertile, with the smooth muscle myosin heavy chain (smMHC or Myh11) promoter directing bicistronic Cre and EGFP protein expression to smooth muscle cells during development as well as in the adult mouse. Hemizygotes from founder line SMCG2 (SM2Cre/GFP) display intense EGFP fluorescence restricted to vascular and nonvascular smooth muscle, with strong concordance between cre expression and EGFP fluorescence (verifying the use of fluorescence as a marker for conditional gene recombination). When bred with mice containing a loxP-flanked sequence of interest, the resulting offspring can have Cre-mediated recombination of the flanked sequence in smooth muscle. Homozygotes are viable and fertile, with smaller litter sizes and a higher incidence of perinatal mortality. These smMHC/Cre/eGFP transgenic mice may be useful in studies utilizing "Cre-lox" technology or fluoresc ..... For more information please see the full phenotype on the strain data sheet | ||
| 005657 | B6.Cg-Tg(Myh6-cre/Esr1)1Jmk/J | Repository- Live |
| Mice that are homozygous for this "MerCreMer" double fusion protein are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre expression in heart tissue is confirmed by western blot. Southern blot confirmed heart cell specificity compared to brain, kidney, lung, liver, and skeletal muscle. Insertion of this transgene and its protein show no changes in echocardiography, heart mass or pathology, or hypertrophy marker genes compared to nontransgenic littermates. Of note, this double fusion protein has substantially greater Cre recombinase activity with less promiscuity compared with a single CreMer fusion protein. As the cre is flanked on each end with a mutated murine estrogen receptor ligand binding domain (amino acids 281-599, G525R); Cre expression is tamoxifen inducible yet estrogen insensitive. Inducible expression of cre in cardiac cells makes this strain suitable for creating bitransgenic mice for use in studies of te ..... For more information please see the full phenotype on the strain data sheet | ||
| 008205 | B6.Cg-Tg(NPHS2-cre)295Lbh/J | Repository- Live |
| Podocin-Cre mice (mice harboring the p2.5P-Cre transgene) are viable and fertile, with expression of Cre recombinase directed to podocytes within kidney glomeruli by the human podocin (NPHS2) promoter/enhancer region. Cre-recombinase activity is reported in podocytes during late capillary loop stage of glomerular development and persists in podocytes of mature glomeruli, with no evidence for cre expression detected in other tissues examined. Embryonic Cre-recombinase activity is also reported as early as 8.5 dpc. When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the sequence. These Podocin-Cre mice (mice harboring the p2.5P-Cre transgene) may be useful in generating conditional knockouts for studying the role of podocyte nephrobiology in renal disorders. | ||
| 003771 | B6.Cg-Tg(Nes-cre)1Kln/J | Repository- Live |
| These transgenic mice express Cre recombinase under the control of the rat nestin promoter and enhancer. Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Initial studies utilizing a reporter strain carrying a beta galactosidase transgene whose expression is dependent on Cre-mediated recombination indicate that cre is primarily expressed in the central and peripheral nervous system with a few isolated kidney and heart cells also expressing activity. The donating investigator indicates that Cre recombinase activity is present in nervous tissue by embryonic day 11.
View cre expression characterization. | ||
| 005975 | B6.Cg-Tg(Plp1-cre/ESR1)3Pop/J | Repository- Live |
| These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse Plp1, proteolipid protein (myelin) 1 promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration allows for ablation of predetermined genes in oligodendrocytes and Schwann cells, and will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice. M ..... For more information please see the full phenotype on the strain data sheet | ||
| 005584 | B6.Cg-Tg(Prrx1-cre)1Cjt/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the paired related homeobox 1 promoter. Cre recombinase expression closely patterns endogenous gene expression and is detectable by embryonic day 9.5. Some recombination occurs in the female germline. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in early limb bud mesenchyme. This strain represents an effective tool for generating tissue specific-targeted mutants useful in studies of limb bud development and patterning. | ||
| 008454 | B6.Cg-Tg(Sox2-cre)1Amc/J | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse SRY-box containing gene 2 promoter. When crossed to a reporter line, Beta-galactosidase activity is detected in the epiblast cells at embryonic day 6.5, with little or no activity in other cells at gastrulation. Some activity is also detected in extra embryonic derivatives of the epiblast, the yolk sac mesoderm and amnion. No activity is detected in primitive endoderm derived tissues, visceral endoderm. The Donating Investigator has not attempted to make the strain homozygous. This strain represents an effective tool for generating epiblast-derived specific targeted mutants. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first ..... | ||
| 006361 | B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J | Repository- Live |
| Mice hemizygous for this Osx1-GFP::Cre transgene are viable and fertile.
The transgene carries both tTA under the regulation of the osterix (Sp7) promoter and, just downstream, a tetracycline responsive element (TRE; tetO)-controlled GFP/Cre fusion protein. In the absence of the tetracycline analog doxycycline, EGFP-Cre fusion protein expression is restricted to the osteoblast lineage throughout embryonic and early postnatal development. Fusion protein activity is largely absent from chondrocytes. When these transgenic animals are mated to transgenic strains that carry loxP-flanked (floxed) conditional alleles, Cre-mediated recombination of the floxed allele in the double mutant animals is placed under the regulation of doxycycline (dox) such that dox adminstration prevents fusion protein expression and recombination. The donating investigator suggests that the mice be maintained on dox-treated water to avoid incidental effects of tTA expression. These Osx1-GFP::Cre mut ..... For more information please see the full phenotype on the strain data sheet | ||
| 004128 | B6.Cg-Tg(Tek-cre)12Flv/J | Repository- Live |
| These transgenic mice express Cre recombinase under the direction of the receptor tyrosine kinase Tek promoter/enhancer, which has been shown to provide uniform expression in endothelial cells during embryogenesis and adulthood. Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity results in the deletion of loxP flanked targets in the female germline as well as in endothelial cells and hematopoietic cells. A low frequency of deletion events are also observed by inheritance from the male germline. | ||
| 008863 | B6.Cg-Tg(Tek-cre)1Ywa/J | Repository- Live |
| These transgenic mice express Cre recombinase under the direction of the receptor tyrosine kinase Tek (Tie2) promoter/enhancer, which has been shown to provide uniform expression in endothelial cells during embryogenesis and adulthood. Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity results in the deletion of loxP flanked targets in vascular endothelial cells. | ||
| 008601 | B6.Cg-Tg(Th-cre)1Tmd/J | Repository- Live |
| Mice hemizygous for the TH-Cre transgene are viable and fertile, with the rat tyrosine hydroxylase (TH) promoter directing expression of Cre recombinase to catecholaminergic cells. Using crosses to reporter strains, cre activity is confirmed in catecholaminergic cells and is present in many of the projection areas of these neuronal populations. A mosaic of cre activity is noted in TH-positive neurons. Several other areas that are not typically thought to have active TH expression, including the lateral septal nucleus, accessory olfactory bulb, suparafascicular thalamus, and pretectal area, also exhibit Cre recombinase activity (possibly as a result of TH promoter activity in precursor cell populations or ectopic expression from the exogenous TH promoter). Some TH-negative cells closely clustered around and within TH-positive nuclei demonstrate cre activity. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will re ..... For more information please see the full phenotype on the strain data sheet | ||
| 007901 | B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J | Repository- Live |
| These Thy1-Brainbow 1.0 (line H) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Prior to Cre-mediated recombination, the fluorescent protein immediately adjacent to the promoter, tdimer2(12) (RFP), is expressed in peripheral and central neurons. When bred to Cre recombinase expressing mice, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): tdimer2(12) (RFP) (no recombination), mYFP, or mCerulean (CFP). A palmitoylation sequence tethers the mYFP and mCerulean (CFP) to the membrane, allowing clear labeling of axonal processes, while cytoplasmic tdimer2(12) (RFP) better labeled neuronal cell bodies and dendrites. Integration of tandem transgen ..... For more information please see the full phenotype on the strain data sheet | ||
| 007911 | B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J | Repository- Live |
| These Thy1-Brainbow 1.1 (line M) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Although the fluorescent protein immediately adjacent to the promoter, Kusabira-Orange (kOFP), was designed to be expressed prior to Cre-mediated recombination, basal kOFP expression is not observed in mouse tissues. When bred to Cre recombinase expressing mice, however, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): mCerulean (CFP), mYFP, or mCherry (RFP). The resulting fluorescent protein expression is observed in astrocytes of all areas of the brain and spinal cord, as well as dentate gyrus granule cells. A palmitoylation sequence tethers the mCherry (RFP), ..... For more information please see the full phenotype on the strain data sheet | ||
| 007921 | B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J | Repository- Live |
| These Thy1-Brainbow 2.1 (line R) transgenic mice are viable and fertile. The mice possess two invertible DNA segments (four fluorescent protein sequences in total) uniquely positioned in tandem and flanked with LoxP sites to generate a larger number of recombination outcomes; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Although the fluorescent protein immediately adjacent to the promoter, hrGFPII (with nuclear localization signal), was designed to be expressed prior to Cre-mediated recombination, basal hrGFPII expression may not be observed in mouse tissues. When bred to Cre recombinase expressing mice, however, the resulting offspring can have one of three different inversions for each transgene in each cell of the cre expressing tissue(s). In addition, two excision events may reduce the construct to one of two single invertible DNA segments which can continue to invert as long as cre is present. These different recomb ..... For more information please see the full phenotype on the strain data sheet | ||
| 007606 | B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J | Repository- Live |
| These transgenic mice ("SLICK" for single-neuron labeling with inducible Cre-mediated knock-out) have a tamoxifen inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted neuron-specific deletions only in neurons where the Thy1 promoter is active. These cells will be evident by the expression of Yellow Fluorescent Protein. Transgenic mice express YFP constitutively in neurons. The Donating Investigator reports that founder line A mice express the transgene in small subsets of motor neurons and dorsal root ganglion neurons.
..... For more information please see the full phenotype on the strain data sheet | ||
| 008085 | B6.Cg-Tg(UBC-cre/ESR1)1Ejb/J | Repository- Live |
| Mice hemizygous for this Cre-ERT2 transgene are viable and fertile. Mice from this founder line have strong tamoxifen-inducible cre activity in all reported tissue types. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to OHT. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in widespread cel ..... For more information please see the full phenotype on the strain data sheet | ||
| 008610 | B6.Cg-Tg(Vav1-cre)A2Kio/J | Repository- Live |
| Mice hemizygous for this Vav-iCre transgene are viable and fertile, with the mouse HS21/45-vav control regions directing expression of an optimized variant of Cre recombinase (iCre) to hematopoietic cells (and their progenitors). Using crosses to a reporter strain, variegated germ line (testis and ovaries), and heart and gut expression is also reported. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Vav-iCre transgenic mice may be useful for generating conditional mutations in hematopoietic cells.
Of note, this Vav-iCre strain (Stock No. 008610) allows reliable deletion of specific genes throughout the entire hematopoietic compartment, whereas the hCD2-iCre strain (Stock No. 008520) allows targeting to be focused to T cells and B cells. | ||
| 006234 | B6.Cg-Tg(tetO-cre)1Jaw/J | Repository- Live |
| Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of a tetracycline-responsive promoter element (TRE; tetO). When hemizygotes are bred with another transgenic mouse expressing either reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under the control of tissue-specific promoters, Cre recombinase expression and Cre-mediated recombination in the appropriate tissues of the bitransgenic offspring can be regulated with the tetracycline analog, doxycycline. This strain represents an effective tool for generating inducible tissue specific-targeted mutants to study cell lineage during development.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was fi ..... | ||
| 006475 | B6.FVB(129S4)-Tg(Ckmm-cre)5Khn/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice have the Cre recombinase gene driven by the muscle creatine kinase (MCK or Ckm) promoter. Cre activity is observed in skeletal and cardiac muscle. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in skeletal and cardiac muscle deletion of the flanked genome. | ||
| 006451 | B6.FVB(129X1)-Tg(Sim1-cre)1Lowl/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Transgene expression is observed in all areas that endogenously express Sim1, including paraventricular hypothalamus and other parts of the brain. When these Sim1-Cre mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequences in Sim1-expressing tissues (including hypothalamus). As such, Sim1-Cre transgenic mice may be useful in studying body weight homeostasis, obesity, leptin metabolism, or as a reporter strain for Sim1-transcription factor activity.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the st ..... | ||
| 006333 | B6.FVB(Cg)-Tg(Neurog3-cre)C1Able/J | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Expression of the transgene is directed by a neurogenin 3 promoter. Tissues where Cre recombinase expression is detected include the small intestine (base of intestinal crypts) and fetal pancreatic epithelial cells. Cre activity has been shown in islets of the adult pancreas, small intestine enteroendocrine cells, endocrine portions of the stomach, all pancreatic endocrine cells, and in some non-endocrine intestinal cells. When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked gene in the tissues that normally express neurogenin 3.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be not ..... | ||
| 003724 | B6.FVB-Tg(EIIa-cre)C5379Lmgd/J | Repository- Live |
| This line carries a cre transgene under the control of the adenovirus EIIa promoter that targets expression of Cre recombinase to the early mouse embryo. Cre expression is thought to occur prior to implantation in the uterine wall. A mosaic pattern of expression is commonly observed. Cre-mediated recombination occurs in a wide range of tissues, including the germ cells that transmit the genetic alteration to progeny. These mice may be useful for breeding to other mice carrying loxP-flanked DNA sequences of interest. This would readily generate progeny in which Cre-mediated excision of the targeted sequences has occurred.
View cre expression characterization. | ||
| 006660 | B6.SJL-Slc6a3tm1.1(cre)Bkmn/J | Repository- Live |
| Mice homozygous for this dopamine transporter IRES-cre (DATIREScre) mutant allele are viable and fertile. Cre recombinase activity is observed as early as embryonic day 15, and co-localizes with endogenous gene expression in adult dopaminergic cell groups (substantia nigra (SN) and ventral tegmental area (VTA), as well as in the retrorubral field). Lesser Cre recombinase activity occurs in adult olfactory bulb glomeruli, mimicking the known lower Slc6a3 (or DAT) expression in this tissue. Although the pattern and intensity of DAT immunostaining in the SN, VTA and striatum do not differ between wild-type and mutant mice, striatum DAT protein levels are moderately reduced (17%) in heterozygotes and significantly reduced (47%) in homozygotes. This diminution in homozygous striatum is associated with significantly increased neuropeptide PDyn (but not D1, D2, or PPE) mRNA levels compared to wild-type, while such an increase is not observed in heterozygotes. When bre ..... For more information please see the full phenotype on the strain data sheet | ||
| 004586 | B6.SJL-Tg(Vil-cre)997Gum/J | Repository- Live |
| Mice hemizygous for this transgene express Cre recombinase under the direction of the mouse villin 1 promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in villi and crypt cells of the small and large intestines, closely patterning the endogenous gene expression. The Donating Investigator indicates that expression is generally continuous, but that a small amount of mosaicism is noted in the colon. Onset of transgene expression is at 12.5 dpc, which is delayed from the endogenous mouse Vil1 gene expression onset of 9.0 dpc. Hemizygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of intestinal organogenesis. | ||
| 006329 | B6;129-Baxtm2Sjk Bak1tm1Thsn/J | Repository- Live |
| Mice homozygous for both alleles (Baxfl and bak-) are viable and fertile with no reported abnormalities. Splenic and thymic tissues display no Bak1 protein expression. When bred to Cre recombinase expressing mice, the resulting offspring will have exons 2-4 of Bax deleted in the cre-expressing tissues (determined by promoter driving cre expression). The conditional deletion of Bax combined with the Bak1 null allele makes these mice useful in studies of apoptosis regulation, tissue homeostasis, and development in multiple cell lineages.
When bred to a strain with a Bak1 targeted null allele (Stock No. 004183) and to either a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 004126) or to a strain expressing interferon inducible Cre recombinase ( ..... | ||
| 008364 | B6;129-Chattm1(cre/Esr1)Nat/J | Repository- Live |
| These targeted mutation mice carry a tamoxifen-inducible Cre cassette knocked into the 3' UTR of the gene. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating 4-hydroxytamoxifen-induced, Cre-mediated targeted deletions specifically in cholinergic neurons. Heterozygotes and homozygotes are normal in size, viability and fertility. | ||
| 008310 | B6;129-Gabrb3tm2.1Geh/J | Repository- Live |
| These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s). When bred to a strain expressing Cre recombinase in the neuronal cells (see Stock No. 003966 for example), this mutant mouse strain may be useful in studies of neurodevelopmental diseases. When bred to a strain expressing Cre recombinase in the hippocampal CA1 pyramidal cell layer (see Stock No. 005359 for example), this mutant mouse strain may be useful in studies of neurodevelopmental diseases. Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations ..... | ||
| 010607 | B6;129-Gm98tm1Barr/J | Repository- Live |
| Mice that are homozygous for the targeted mutation are viable, fertile with loxP sites flanking exon 8 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in cre-expressing tissue(s). This mutant mouse strain may be useful in generating conditional mutations for studying the role of Gm98 in CNS myelination and other cellular processes. | ||
| 008883 | B6;129-Gt(ROSA)26Sortm1(SNCA*A53T)Djmo/TmdJ | Repository- Live |
| Homozygous ROSA26-Syn-A53T mice are viable and fertile, with the familial Parkinson's disease-associated A53T missense mutant form of human alpha-synuclein (human A53T α-Syn or SYNA53T) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of human A53T α-Syn is blocked by an upstream loxP-flanked STOP sequence (in the absence of Cre recombinase, no human A53T α-Syn protein is observed in brain regions). When bred to cre expressing mice, the STOP sequence is deleted in the tissues of offspring where Cre recombinase is present; resulting in human A53T α-Syn expression. In particular, Stock No. 008601 B6.Cg-Tg(Th-cre)1Tmd/J may be useful for this application. These ROSA26-Syn-A53T mice allow inducible expression of a human mutation associated with familial Parkinson's disease and may be useful for studying the progressive dopaminergic neurodegeneration of Parkinson's dise ..... For more information please see the full phenotype on the strain data sheet | ||
| 004847 | B6;129-Gt(ROSA)26Sortm1(cre/Esr1)Nat/J | Repository- Live |
| These R26CreER mutant mice have a tamoxifen-inducible Cre-mediated recombination system driven by the endogenous mouse Gt(ROSA)26Sor promoter. The mutant allele consists of a fusion product involving Cre recombinase and an altered version of the mouse estrogen receptor ligand binding domain. The mutant ligand binding domain does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the CRE/ESR1 protein can only gain access to the nuclear compartment to mediate recombination after exposure to tamoxifen. Tamoxifen administration will also induce Cre recombination in the developing embryos of treated mothers. When crossed with a strain containing a loxP site-flanked sequence of interest, this mutant is useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnor ..... For more information please see the full phenotype on the strain data sheet | ||
| 008516 | B6;129-Gt(ROSA)26Sortm1Joe/J | Repository- Live |
| Homozygous ROSA26 GNZ knock-in mice are viable and fertile, with a nuclear-localized green fluorescent protein/beta-galactosidase fusion protein (GFP-NLS-lacZ or GNZ) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of GNZ is blocked by an upstream loxP-flanked STOP sequence (in the absence of Cre recombinase, no expressed GFP or beta-galactosidase activity is observed in GNZ embryos(E9.5-18.5)). When bred to cre expressing mice, offspring will have the STOP sequence deleted in tissues where Cre recombinase is present. The resulting GNZ fusion protein expression allows for enhanced (single cell level) visualization / resolution. The donating investigator reports that Cre recombinase activity can be visualized by direct GFP fluorescence, but the high resolution nuclear staining of GNZ may be best visualized by immunostaining for either GFP or beta-galactosidase. These ROSA26 GNZ mice are useful as a Cre reporter strain; expressing both G ..... For more information please see the full phenotype on the strain data sheet | ||
| 008889 | B6;129-Gt(ROSA)26Sortm2(SNCA*119)Djmo/TmdJ | Repository- Live |
| Homozygous ROSA26-Syn119 mice are viable and fertile, with the familial Parkinson's disease-associated Syn119 C-terminal truncation of human alpha-synuclein (human α-Syn119 or SynCT119) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of human α-Syn119 is blocked by an upstream loxP-flanked STOP sequence (in the absence of Cre recombinase, no human α-Syn119 protein is observed in brain regions). When bred to cre expressing mice, the STOP sequence is deleted in the tissues of offspring where Cre recombinase is present; resulting in human α-Syn119 expression. In particular, Stock No. 008601 B6.Cg-Tg(Th-cre)1Tmd/J may be useful for this application. These ROSA26-Syn119 mice allow inducible expression of a human mutation associated with familial Parkinson's disease and may be useful for studying the progressive dopaminergic neurodegeneration of Parkinson's disease and ..... For more information please see the full phenotype on the strain data sheet | ||
| 004077 | B6;129-Gt(ROSA)26Sortm2Sho/J | Repository- Live |
| These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted into the Gt(ROSA)26Sor locus. Expression of the EGFP gene is blocked by a loxP-flanked STOP fragment placed between the EGFP sequence and the Gt(ROSA)26Sor promoter. This strain serves as a reporter strain, with successful Cre excision being indicated by EGFP expression in cre-expressing tissues. Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The donating investigator indicates that the EGFP expression level in this reporter strain is suitable for applications involving FACS but is too low for histological applications. | ||
| 008886 | B6;129-Gt(ROSA)26Sortm3(SNCA*E46K)Djmo/TmdJ | Repository- Live |
| Homozygous ROSA26-Syn-E46K mice are viable and fertile, with the E46K missense mutant form of human alpha-synuclein (human E46K α-Syn or SYNE46K; associated with familial Parkinson's disease, dementia, and visual hallucinations) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of human E46K α-Syn is blocked by an upstream loxP-flanked STOP sequence (in the absence of Cre recombinase, no human E46K α-Syn protein is observed in brain regions). When bred to cre expressing mice, the STOP sequence is deleted in the tissues of offspring where Cre recombinase is present; resulting in human E46K α-Syn expression. In particular, Stock No. 008601 B6.Cg-Tg(Th-cre)1Tmd/J may be useful for this application. These ROSA26-Syn-E46K mice allow inducible expression of a human mutation associated with familial Parkinson's disease, dementia, and visual hallucinations and may be usefu ..... For more information please see the full phenotype on the strain data sheet | ||
| 008876 | B6;129-Hprttm11(Ple176-EGFP/cre)Ems/J | Repository- Live |
| These Ple176-EGFPCre;mEMS759 mice have the Ple176-EGFPCre transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase 1 (Hprt1) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the human regulator of G-protein signaling 16 (RGS16) gene directing expression of an enhanced green fluorescent protein/Cre recombinase fusion protein (EGFPCre). These Ple176-EGFPCre;mEMS759 mice may be useful in studying RGS16-expressing cells in the brain and diseases affecting the brain.
For Ple176-EGFPCre;mEMS759 expression information, see The Pleiades Promoter Project website (Ple176 Promoter (pEMS1088)). The donating investigator reports: | ||
| 004605 | B6;129-Itgb1tm1Efu/J | Repository- Live |
| These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
When bred to a strain expressing Cre recombinase in the epithelial cells of the intestine (see Stock No. 004586 for example), this mutant mouse strain may be useful in studies of intestinal hyperplasia. | ||
| 008475 | B6;129-Nlgn3tm1Sud/J | Repository- Live |
| These mice carry an R451C mutation in exon 7 of the gene. mRNA is detected by real-time PCR analysis of brain from homozygous animals. Mutant mice exhibit enhancements in inhibitory synaptic transmission as well as spacial learning and memory, but show deficits in social interaction. This mutant mouse strain may be useful in studies of the pathophysiology of autism. Exon 7 is additionally flanked by loxP sites. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical abnormalities. | ||
| 005549 | B6;129-Pax3tm1(cre)Joe/J | Repository- Live |
| This strain expresses Cre recombinase from the endogenous Pax3 locus. Expression of the targeted gene product (mRNA and protein) mimics endogenous gene expression as detected by in situ hybridization and immunohistochemistry of homozygous embryos aged E12.5. No endogenous Pax3 gene product (protein) is detected in homozygotes and approximately one half of the endogenous gene product (protein) is detected in heterozygotes by Western blot analysis. Cre recombinase expression is detected in the dorsal neural tube and somites of E9 to 11.5 embryos and in the cardiac neural crest cells and colonic epithelia of E11.5 embryos. Recombination occurs in neural crest and somite derivatives of later gestation embryos. Homozygous mice have an embryonic lethal phenotype, failing to develop past embryonic day 18.5. At age E13.5 homozygous embryos display severe cardiac and neural tube defects (exencephaly), absent limb musculature and reduced or absent dorsal root ganglia. Heterozygous ..... For more information please see the full phenotype on the strain data sheet | ||
| 008041 | B6;129-Sirt1tm1Ygu/J | Repository- Live |
| Mice homozygous for this targeted allele (SirT1co/co) are viable and fertile. A loxP-flanked neomycin cassette just upstream of exon 4 and a third loxP site downstream of exon 4 were inserted to create this targeted mutant Sirt1 allele. The floxed mutation does not affect SIRT1 protein expression in MEFs or mammary gland tissue in homozygotes. When bred to mice that express Cre recombinase, the resulting offspring have exon 4 (encoding an evolutionarily conserved Sir2 motif) deleted in cre-expressing tissue(s); (the donating investigator reports only one recombination event: complete removal of the neomycin cassette and exon 4, leaving a single loxp). These SirT1co/co mice may be useful in generating conditional mutants for studying transcriptional regulation and the role of estrogen, insulin growth factor-1 (IGF-1), and transcription factors (including NF-kappaB) in mammary gland development, mammary cancer, apoptosis, and metabolic di ..... For more information please see the full phenotype on the strain data sheet | ||
| 005650 | B6;129-Tg(Myh6-cre/Esr1)1Jmk/J | Repository- Live |
| Mice that are homozygous for the transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre expression in heart tissue is confirmed by western blot. Southern blot confirmed heart cell specificity compared to brain, kidney, lung, liver, and skeletal muscle. Insertion of this transgene and its protein show no changes in echocardiography, heart mass or pathology, or hypertrophy marker genes compared to nontransgenic littermates. Of note, this double fusion protein has substantially greater Cre recombinase activity with less promiscuity compared with a single CreMer fusion protein. Inducible expression of cre in cardiac cells makes this strain suitable for creating bitransgenic mice for use in studies of temporally regulated deletion of loxP-flanked targeted genes. | ||
| 007605 | B6;129P-Psen1tm1Vln/J | Repository- Live |
| These mice possess loxP sites on either side of exon 7 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these "floxed" mice are bred to mice that express Cre recombinase, resulting offspring can have one of three resulting genotypes (only exon 7 deleted, only the neo selection cassette deleted, or both exon 7 and the neo selection cassette deleted) in the cre-expressing tissue(s). These PS1-floxed mice may be useful in generating conditional knockouts of Presenilin 1 for studying Alzheimer's Disease.
For example, when crossed to a strain expressing Cre recombinase in postnatal neurons (see Stock No. 006143), this mutant mouse strain may be useful in studies of amyloid plaque formation. | ||
| 008529 | B6;129P-Tg(Neurog1-cre/ESR1)1Good/J | Repository- Live |
| These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse neurogenin 1, Neurog1, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/ESR1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in the cortex, hippocampus, thalamus, hypothalamus and cochlear-vestibular ganglion. Hemizygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnorma ..... For more information please see the full phenotype on the strain data sheet | ||
| 006847 | B6;129P2-Mecp2tm1Bird/J | Repository- Live |
| These mice possess two functional loxP sites flanking exons 3-4 of the targeted gene on the X chromosome. Homozygous females and hemizygous males are viable and fertile. Northern blot analysis showed the expected mature transcript from the Mecp2lox locus. Also detected was an unspliced beta-globin transcript that was introduced into the locus as part of the targeting vector. When these mutant mice are bred to mice that express cre recombinase, resulting offspring will have exons 3-4 deleted in the cre-expressing tissue(s). Mice with this X-linked floxed mutation may be useful in neurological and developmental studies of Rett syndrome. For example, when crossed to a strain expressing Cre recombinase in nervous tissue (see Stock No. 003771), this mutant mouse strain develops a neurological phenotype that mimics Rett syndrome. | ||
| 008069 | B6;129P2-Pvalbtm1(cre)Arbr/J | Repository- Live |
| This strain expresses Cre recombinase from the endogenous Pvalb, parvalbumin, locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in more than 90% of neurons that express parvalbumin, such as interneurons in the brain and proprioceptive afferent sensory neurons in the dorsal root ganglia. This mutant mouse strain represents a model that may be useful in studies of neuronal differentiation. | ||
| 007001 | B6;129S-Tg(UBC-cre/ESR1)1Ejb/J | Repository- Live |
| Mice hemizygous for this Cre-ERT2 transgene are viable and fertile. Mice from this founder line have strong tamoxifen-inducible cre activity in all reported tissue types. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to OHT. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in widespread cel ..... For more information please see the full phenotype on the strain data sheet | ||
| 009389 | B6;129S1-Bambitm1Jian/J | Repository- Live |
| Mice homozygous for this Bambiflox allele are viable and fertile, with loxP sites flanking exon 1 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the endogenous signal peptide deleted in the cre-expressing tissue(s); this is expected to produce a null allele. These mutant mice may be useful in generating conditional mutations for studying the role of Bambi in developmental biology and the TGF-beta pathway. | ||
| 009388 | B6;129S1-Osr2tm2(cre)Jian/J | Repository- Live |
| Mice homozygous for the Osr2-IresCre (or Osr2IresCre) allele are viable and fertile, with an IRES-Cre bicistronic expression cassette inserted into the 3' UTR of the targeted locus. As such, cre expression is directed by the endogenous promoter/enhancer regions primarily to developing palate mesenchyme and metanephric mesenchyme-derived glomeruli tissues (but not other epithelial and mesenchymal tissues in the developing metanephric kidney). Some ectopic cre activity is reported (particularly in the central nervous system). When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence in these tissues in the offspring. These Osr2-IresCre mice may be useful for generating conditional mutations for studying developmental biology (palate and kidney development). | ||
| 006414 | B6;129S4-Mc4rtm1Lowl/J | Repository- Live |
| The mice have a loxp-flanked transcriptional blocking (loxTB) sequence that prevents normal endogenous gene transcription and translation from the endogenous locus. As such, homozygous mice are devoid of functional mRNA in all tested regions of the brain. Homozygous mice exhibit severe early-onset obesity, accompanied by hyperphagia, increased snout-anus length and hyperinsulinemia. The function of this disrupted allele can be restored by the enzymatic activity of Cre-recombinase. These mutant mice may be useful in studies of neurobiology, obesity, diabetes, hunger/appetite, and fat and energy metabolism.
When bred to a strain expressing Cre recombinase in the hypothalamus see Stock No. 006395 for example), this mutant mouse strain exhibits as intermediate phenotype in comparison to homozygous null mice. | ||
| 007845 | B6;129S4-Myf5tm3(cre)Sor/J | Repository- Live |
| This strain expresses Cre recombinase from the endogenous Myf5 locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs skeletal muscle and the dermis, and in several ectopic locations. Homozygotes for this allele have a perinatal lethal phenotype and die at birth. Homozygotes display abnormal rib development and some fusions of the cervical or thoracic vertebrae. This mutant mouse strain represents a model that may be useful in studies of skeletal development. | ||
| 008361 | B6;129S4-Trp53tm5Tyj/J | Repository- Live |
| These targeted mutant mice carry a loxP-flanked STOP cassette in intron 1 that blocks expression of the gene. Both heterozygotes and homozygotes are prone to the development of tumors (primarily spontaneous thymic lymphomas and sarcomas). The latency is significantly shorter in homozygotes than in wildtype mice. Homozygotes have reduced fertility. Upon Cre-mediated recombination, the STOP cassette can be excised, leading to restoration of gene expression. This strain permits temporal control of this tumor suppressor gene, and enables studies of tumorigenesis. | ||
| 009357 | B6;129S6-Adam10tm1Zhu/J | Repository- Live |
| These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissues. When bred to a strain expressing Cre recombinase in T cells for example(see Stock No. 003802), this mutant mouse strain may be useful in studies related to thymocyte developmental defects. | ||
| 006410 | B6;129S6-Chattm1(cre)Lowl/J | Repository- Live |
| Homozygous mice maintained at The Jackson Laboratory are viable and fertile. An "IRES-Cre" sequence is inserted downstream of the stop codon such that cre expression is controlled by the endogenous Chat gene promoter. Chat gene expression, however, is unaffected. Cre recombinase activity is reported in all cholinergic neurons. These mice may be useful for "Cre-lox" technology applications in neurobiology, including studies of motor function, learning and memory, Alzheimer's disease, and Down syndrome, and in obesity and diabetes research.
View cre expression characterization. | ||
| 007908 | B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | Repository- Live |
| Ai14 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE::deltaNeo conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. Ai14 mice are not expected to express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre is expected to be detectable by fluorescence and mRNA (in situ hybridization). These Ai14 mice are useful as a Cre reporter strain; expressing the red fluorescent protein variant, tdTomato, following Cre-mediated recombination.
Of note, ..... | ||
| 007905 | B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J | Repository- Live |
| Ai9 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai9 mice do not express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre can be detected by fluorescence and mRNA (in situ hybridization). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-mediated recombination if ..... For more information please see the full phenotype on the strain data sheet | ||
| 009363 | B6;129X1-Cdc25atm1Hpw/J | Repository- Live |
| These mice possess loxP sites on either side of exons 1-3 in the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissues. (widespread deletion of expression is embryonic lethal). This strain may be useful in studies of development, cell cycles, and cancer in adult mice. | ||
| 008467 | B6;129X1-Wnt7btm2Amc/J | Repository- Live |
| Mice homozygous for the Wnt7bc3 allele are viable and fertile, with loxP sites flanking exon 3 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). Unlike other Wnt7b mutant alleles, this Wnt7bc3 conditional allele is not affected by alternative exon 1 splicing. These Wnt7bc3 mice may be useful in generating conditional mutations for studying the role of Wnt7b (and other Wnt family members) in development and canonical Wnt signaling cascades, including lung differentiation and growth. In addition, these mice may also be useful in conjunction with other Wnt7 mutant strains including Wnt7b knockout mice (Stock No. 004693) and Wnt7a mutant mice (Stock No. 004715).
When bred to a strain expressing Cre recom ..... | ||
| 008605 | B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J | Repository- Live |
| Hemizygous IRG transgenic mice are viable and fertile, with widespread expression of a loxP-flanked optimized red fluorescent protein variant (DsRed-Express) directed to embryonic and adult tissues by the CAG promoter prior to exposure to Cre recombinase. When bred to cre-expressing mice, the resulting offspring have the DsRed-Express cassette deleted in the cre-expressing tissue(s), allowing expression of the enhanced green fluorescent protein (EGFP) cassette located just downstream. These IRG transgenic mice are a double-fluorescent, Cre-reporter strain; with widespread expression of red fluorescence prior to Cre recombinase exposure, and green fluorescence following cre-mediated recombination in a pattern determined by cre expression, and should provide a versatile tool for analyzing complex cellular relationships in a wide variety of tissues. For example, when using IRG transgenic mice along with Nestin-Cre mice (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 008844 | B6;C3-Tg(Ctgf-cre)2Aibs/J | Repository- Live |
| Hemizygous Ctgf-Tg2-Cre mice are viable and fertile, with cre expression directed to cortex and hippocampus by the Ctgf promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Ctgf-Tg2-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex and hippocampus).
For characterization information, see images at the Allen Institute for Brain Science website (Ctgf-Tg2-Cre images (1), Ctgf-Tg2-Cre images (2), and For more information please see the full phenotype on the strain data sheet | ||
| 008839 | B6;C3-Tg(Cyp39a1-cre)1Aibs/J | Repository- Live |
| Hemizygous Cyp39a1-Tg1-Cre mice are viable and fertile, with cre expression directed to cortex, hippocampus, striatum, olfactory bulb and cerebellum by the Cyp39a1 promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Cyp39a1-Tg1-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex, hippocampus, striatum, olfactory bulb and cerebellum).
For characterization information, see images at the Allen Institute for Brain Science website (Cyp39a1-Tg1-Cre images (1), For more information please see the full phenotype on the strain data sheet | ||
| 009117 | B6;C3-Tg(Cyp39a1-cre)7Aibs/J | Repository- Live |
| Hemizygous Cyp39a1-Tg7-Cre mice are viable and fertile, with cre expression directed to cortex, hippocampus and cerebellum by the Cyp39a1 promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Cyp39a1-Tg7-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex, hippocampus and cerebellum).
For characterization information, see images at the Allen Institute for Brain Science website. | ||
| 009111 | B6;C3-Tg(Scnn1a-cre)1Aibs/J | Repository- Live |
| Hemizygous Scnn1a-Tg1-Cre mice are viable and fertile, with cre expression directed to cortex, striatum, hippocampus and cerebellum by the Scnn1a promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Scnn1a-Tg1-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex, striatum, hippocampus and cerebellum).
For characterization information, see images at the Allen Institute for Brain Science website (for example, Scnn1a-Tg1-Cre ima ..... | ||
| 009112 | B6;C3-Tg(Scnn1a-cre)2Aibs/J | Repository- Live |
| Hemizygous Scnn1a-Tg2-Cre mice are viable and fertile, with cre expression directed to cortex, thalamus, midbrain and cerebellum by the Scnn1a promoter/enhancer regions within the BAC transgene. The donating investigators may not have assessed expression in tissues other than brain. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. These Scnn1a-Tg2-Cre mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex, thalamus, midbrain and cerebellum).
For characterization information, see images at the Allen Institute for Brain Science website (for example, Scnn1a-Tg2-Cre images (1 ..... | ||
| 009103 | B6;C3-Tg(Wfs1-cre/ERT2)3Aibs/J | Repository- Live |
| Hemizygous Wfs1-Tg3-CreERT2 mice are viable and fertile. As the Cre-ERT2 fusion gene is under control of the Wfs1 promoter/enhancer regions within the BAC transgene, cre activity is directed to cortex, hippocampus, striatum, thalamus and cerebellum only following taxomifen administration. The donating investigators may not have assessed expression in tissues other than brain. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone ma ..... For more information please see the full phenotype on the strain data sheet | ||
| 006465 | B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J | Repository- Live |
| These ZW transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread, but mosaic, throughout the central and peripheral nervous systems. Purkinje cells display intense beta-galactosidase activity. Approximately 50% of the total neuron population express the transgene, as detected by beta-galactosidase activity. Newborn mice exhibit widespread beta-galactosidase activity. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with wheat germ agglutinin (plant lectin) expression in tissues expressing cre. The double reporter system makes it possible to distinguish a lack of reporter (lacZ) expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity at the individual cell level. This transgenic mouse strain may be useful in tracing transneuronal or trans-synaptic connections and circuits in brain regions or in the s ..... For more information please see the full phenotype on the strain data sheet | ||
| 007910 | B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J | Repository- Live |
| These Thy1-Brainbow 1.0 (line L) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Prior to Cre-mediated recombination, the fluorescent protein immediately adjacent to the promoter, dTomato (RFP), is expressed in peripheral and central neurons. When bred to Cre recombinase expressing mice, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): dTomato (RFP) (no recombination), mCerulean (CFP), or mYFP. Integration of tandem transgene copies yields combinatorial fluorescent protein expression in each cell, and thus many possible cell colors, providing a way to distinguish adjacent neurons and visualize other cellular interactions. Of note, the singl ..... For more information please see the full phenotype on the strain data sheet | ||
| 008533 | B6;FVB-Tg(Cspg4-cre)1Akik/J | Repository- Live |
| Mice hemizygous for the NG2creBAC transgene are viable and fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the Cre recombinase under the control of the mouse NG2 (Cspg4) promoter/enhancer. Cre recombinase expression is detected in NG2 expressing glial cells and vasculature throughout the brain as well as in NG2-expressing cells in other tissues from late embryonic stages (~embryonic day 14) throughout adulthood. The efficiency of Cre-mediated recombination is less than 100% (86% in forebrain and 70-75% in spinal cord). Immunoreactive Cre was not detected in S100beta+ or GFAP+ astrocytes or APC+ oligodendrocytes. These NG2creBAC transgenic mice may be may be crossed to various floxed mutants to delete floxed sequences specifically in NG2-expressing cells. The donating investigator reports that there appears to be transient Cre expression in some projection neurons and interneurons scattered throughout the ce ..... For more information please see the full phenotype on the strain data sheet | ||
| 005249 | B6;SJL-Tg(Krt1-15-cre/PGR)22Cot/J | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice have a synthetic steroid RU 486 inducible Cre-mediated recombination system driven by the mouse keratin complex 1, acidic, gene 15 promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the human progesterone receptor. The mutant human progesterone receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, RU 486. Restricted to the cytoplasm, the Cre/PGR protein can only gain access to the nuclear compartment after exposure to RU 486. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating RU 486-induced, Cre-mediated targeted deletions. This strain represents an effective tool for generating tissue-specific targeted mutants that would be useful to study epi ..... For more information please see the full phenotype on the strain data sheet | ||
| 007610 | B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J | Repository- Live |
| These transgenic mice ("SLICK" for single-neuron labeling with inducible Cre-mediated knock-out) have a tamoxifen inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted neuron-specific deletions only in neurons where the Thy1 is active. These cells will be evident by the expression of Yellow Fluorescent Protein. Transgenic mice express YFP constitutively in neurons. The Donating Investigator reports that founder line V mice express the transgene sparsely in neurons of the central nervous system.
This strain is one of ..... | ||
| 003465 | BALB/c-Tg(CMV-cre)1Cgn/J | Repository- Live |
| In this transgenic strain, deletion of loxP-flanked genes occurs in all tissues, including germ cells. The cre gene in this strain is under the transcriptional control of a human cytomegalovirus minimal promoter and is likely to be expressed before implantation during early embryogenesis. It also appears that the cre gene is X-linked since transgene transmission through males is restricted to female offspring. As these cre-transgenic mice are on a BALB/c background, they are ideally suited for breeding with gene-targeted mutant mice that have been created using the BALB/c-derived ES cell line BALB/c-I.
View cre expression characterization. | ||
| 008879 | C.B6-Arg1tm1Pmu/J | Repository- Live |
| These mice possess loxP sites on either side of exons 7 and 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 7 and 8 deleted in the cre-expressing tissue(s).
When bred to a strain with inducible Cre recombinase expression in the myeloid cell lineages (see Stock No. 004781, for example ) or endothelial cells (see Stock No. 004128 , for example), this mutant mouse strain may be useful in studies of immune response to bacterial and parasitic infections. | ||
| 004126 | C.Cg-Cd19tm1(cre)Cgn Ighb/J | Repository- Live |
| The Cd19 promoter specifically directs expression at the earliest stages and throughout B-lymphocyte development and differentiation. A Cre cassette is inserted into the Cd19 exon 2, functionally disrupting the gene. Homozygous mice are Cd19-deficient, whereas heterozygous mice are phenotypically normal and can be used for specific deletion of floxed targets in B-lymphocytes. Mice that are homozygous deficient for Cd19 are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. A deficiency in the B-1 subset of B-lymphocytes is observed along with a concomitant reduction in serum IgM. Homozygous mice are severely impaired in their ability to respond to T-cell-dependent antigens and fail to form splenic germinal centers. | ||
| 008817 | C57BL/6-Arg1tm1Pmu/J | Repository- Live |
| These mice possess loxP sites on either side of exons 7 and 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 7 and 8 deleted in the cre-expressing tissue(s).
When bred to a strain with inducible Cre recombinase expression in the myeloid cell lineages (see Stock No. 004781, for example) or endothelial cells (see Stock No. 004128 , for example), this mutant mouse strain may be useful in studies of immune response to bacterial and parasitic infections. | ||
| 009155 | C57BL/6-Cldn6tm1(cre)Dkwu/J | Repository- Live |
| This targeted strain expresses Cre under the control of the claudin 6 promoter. When bred to a floxed reporter strain, broad expression throughout the epiblast is seen at E6.5. By E9.5, expression is restricted to the endoderm-derived structures such as the oral cavity, digestive tract, and lung. In addition, it is expressed in mesonephros and otic vesicle. Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. | ||
| 007900 | C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J | Repository- Live |
| Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.
For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation. | ||
| 008242 | C57BL/6-Gt(ROSA)26Sortm1(Ikbkb)Rsky/J | Repository- Live |
| Mice homozygous for the R26StopFLikk2ca conditional allele are viable and fertile, with a loxP-flanked STOP cassette preventing transcription of the downstream bicistronic sequences (a FLAG-tagged, constitutively active form of IKbkb (IKK2ca) and EGFP). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of the IKK2ca as well as EGFP fluorescence. Expression of IKK2ca leads to constitutively active NF-kappaB transcription factor activity. These R26StopFLikk2ca mice allow inducible expression of an activated form of Ikbkb (IKK2 or IKK-beta) and subsequent activation of the NF-kappaB transcription factor pathways.
For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this ..... | ||
| 008517 | C57BL/6-Gt(ROSA)26Sortm3(CAG-MIRN17-92,-EGFP)Rsky/J | Repository- Live |
| Mice homozygous for the "miR-17-92 transgene" conditional allele are viable and fertile, with a loxP-flanked Neo-STOP cassette preventing transcription of the downstream bicistronic sequences (human miR-17-92 cluster (encoding the precursor of seven miRNA molecules; miR-17-5p, miR-17-3p, miR-18a, miR-19a, miR-20a, miR-19b and miR-92) and EGFP). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of the human miR-17-92 cluster. Because the synthetic CAG promoter driven miR-17-92 transgene was targeted for insertion into the Gt(ROSA)26Sor locus, expression of the transgene is determined by which tissue(s) express Cre recombinase. EGFP fluorescence, however, is not reported following exposure to Cre recombinase (presumably due to RNaseIII excision of the stem-loop structures encoding individual miRNA destabilizing the EGFP portion of the primary transcript ..... For more information please see the full phenotype on the strain data sheet | ||
| 008309 | C57BL/6-Rag2tm1Cgn/J | Repository- Live |
| Mice homozygous for the RAG-2fl allele are viable and fertile, with loxP sites flanking exon 3 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for the entire RAG-2 protein) deleted in the cre-expressing tissue(s). These RAG-2fl mice may be useful in generating conditional mutations for studying the role of RAG-2 in B and T cell development (including cancer and toxicology research as a xenograft/transplant host), T and B cell receptor (V(D)J) recombination, hematopoiesis, hematology, immunology, and inflammation research. For example, when bred to a strain with inducible Cre recombinase expression in liver and lymphocytes (see Stock No. 003556), this mutant mouse strain may be useful in studies of B and T cell development. | ||
| 009369 | C57BL/6-Zbtb7btm1.1Litt/J | Repository- Live |
| These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.
Deletion in CD4+CD8+ thymocytes (through crosses to a Cd4-cre strain) or germline deletion (through EIIa-cre crosses; see Stock No. 003724) causes mice to lose helper T cells. This strain may be useful in studies of T cell lineage commitment. | ||
| 008766 | C57BL/6-Tg(Cd8a-cre)1Itan/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is observed in peripheral CD8+ T cells (CD8 α+CD8β+ αβT cells and CD8α+CD8β- αβT cells, but not in CD4+CD8α-CD8β- αβT cells). When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked genome in cells that normally express Cd8a. | ||
| 006474 | C57BL/6-Tg(Grik4-cre)G32-4Stl/J | Repository- Live |
| Mice hemizygous for this "G32-4" transgene are viable, fertile, and do not display any gross physical or behavioral abnormalities. Transgene expression (cre activity) is detectable at 14 days old in area CA3 of the hippocampus, and at 8 weeks of age, recombination is observed in nearly 100% of pyramidal cells in area CA3. Recombination is also observed in other brain areas, but at distinctly lower frequencies. If bred with mice containing a loxP-flanked sequence of interest, tissue-specific deletion of that sequence results in the offspring. Specifically, when these mice were bred with a conditional CaMKII allele (see Stock No. 006575), the resulting offspring exhibited altered neurotransmitter release. The donating investigator reports that G32-4 females may confer global Cre-mediated deletion of loxP-flanked sequences in some offspring, and recommend using male G32-4 mice exclusively for such trials. These C ..... For more information please see the full phenotype on the strain data sheet | ||
| 008314 | C57BL/6-Tg(HBB-cre)12Kpe/J | Repository- Live |
| These transgenic mice express Cre recombinase under the control of the human beta hemoglobin (HBB) promoter and intron 2-enhancer fragment, and the human beta hemoglobin locus control region (LCR). Cre recombinase expression is detected in blood, brain, gonads, spleen and liver by RT-PCR analysis and in blood by RNAse protection assay analysis. During development Cre activity is restricted to erythroid tissues. The Donating Investigator reports that recombination occurs at 50-100% efficiency in erythroid/megakarycytic cell lineages beginning at onset of hematopoiesis at approximately embryonic day 7.5. When crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in erythroid tissues. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain represents an effective tool for generating tissue speci ..... For more information please see the full phenotype on the strain data sheet | ||
| 008870 | C57BL/6-Tg(Hspa2-cre)1Eddy/J | Repository- Live |
| Mice hemizygous for the Hspa2-Cre transgene are viable and fertile, with expression of Cre recombinase directed to the developing male germ cells by the mouse Hspa2 (heat shock protein 2 [Hsp70-2]) promoter. The Hspa2-Cre sequences of the transgene are flanked by Acrv1 (acrosomal vesicle protein 1 [SP-10]) insulator elements (which are believed to tether the Acrv1 gene to the nuclear matrix in somatic cells [preventing transcription]). When these transgenic mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination is expected to result in deletion of the floxed sequences in leptotene/zygotene spermatocytes of the male offspring. The donating investigator reports that expression of Hspa2-Cre may be "leaky" and recombination of loxP-flanked sequences can occur in some cells in somatic tissues. These Hspa2-Cre mice may be useful to generate conditional mutations for studying mammalian spermatocyte development, g ..... For more information please see the full phenotype on the strain data sheet | ||
| 008535 | C57BL/6-Tg(Pf4-cre)Q3Rsko/J | Repository- Live |
| These transgenic mice express a codon-improved Cre recombinase (iCre) under the control of the mouse Pf4 (platelet factor 4), or Cxcl4, promoter. Cre recombinase expression is detected in the majority of megakaryocytes. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The BAC clone used to generate this strain also contains 4 other genes: Cxcl5, Cxcl7, Cxcl15 and Cxcl3. The additional copy of these chemokine genes in the BAC has no effect on blood count of the mutant mice. This strain represents an effective tool for generating megakaryocyte lineage-restricted specific-targeted mutants. | ||
| 003651 | C57BL/6-Tg(Zp3-cre)93Knw/J | Repository- Live |
| This is a transgenic line in which cre expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene. This promoter normally directs expression exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain would be useful for deleting a floxed sequence specifically in the female germ line. The Donating Investigator suggests to accomplish this, females homozygous or heterozygous for the floxed allele, as well as hemizygous for the Zp3cre allele are crossed with wild type males. Progeny will carry the deleted-floxed allele. | ||
| 006481 | C57BL/6J-Tg(ACTB-NOTCH1)1Shn/J | Repository- Live |
| Transgenic mice are viable, fertile and behaviorally normal. These "CALSL-NICD (H)" mice (or simply CALSL-NICD) reportedly carry 10-20 copies of the transgene inserted into a single genomic locus. Expression of the transgene-derived intracellular domain of human NOTCH1 is prevented by a "Lox-STOP-Lox" cassette. When transgenic mice are bred to a strain expressing Cre recombinase, the "floxed stop" cassette is excised in the resulting offspring, and human NOTCH1 expression is observed in the cre-expressing tissue(s). These transgenic mice may be useful in studying early neural progenitor cell development and apoptosis, and responses to tissue-specific Notch activation. For example, when crossed to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771), this transgenic mouse strain may be useful in studies of notch signaling during apoptotic cell death. | ||
| 007567 | C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J | Repository- Live |
| Mice hemizygous for this CD11c-Cre-GFP transgene are viable and fertile. The CD11c (Itgax) promoter directs bicistronic Cre and EGFP protein expression to dendritic cells (DCs). Expression of EGFP is expected to have equimolar expression with Cre recombinase. When bred with any mouse containing a loxP-flanked sequence of interest, the resulting offspring can have Cre-mediated recombination of the flanked sequence. These CD11c-Cre-GFP transgenic mice (as well as CD11c-Cre transgenic mice (see Stock No. 008068)) may be useful for immunological studies utilizing Cre-lox technology or fluorescent protein expression in dendritic cells. | ||
| 008661 | C57BL/6J-Tg(Nkx2-1-cre)2Sand/J | Repository- Live |
| Mice homozygous for the Nkx2.1Cre (or BAC-Nkx2.1-Cre) transgene are viable and fertile, with cre expression directed to major subgroups of brain interneuron progenitors, developing lung, thyroid, and pituitary by the Nkx2.1 promoter/enhancer regions within the BAC transgene. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in the offspring. For example, when bred with beta-galactosidase reporter mice, the first detectable Cre recombinase expression in double mutant offspring is observed on embryonic day (E)10.5 in the basal telencephalon. These Nkx2.1Cre (or BAC-Nkx2.1-Cre) transgenic mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain progenitors (including future major interneurons in telencephalon and hypothalamus cells), as well as developing lung, thyroid, and pituitary. | ||
| 004081 | C;129S-Vhltm1Jae/J | Repository- Live |
| This strain contains loxP sites flanking the Vhl promoter and exon 1 resulting in a conditional null allele. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cre-mediated recombination results in the deletion of the promoter and exon 1. Studies in which liver-specific inactivation of the Vhl gene was achieved by breeding this strain with albumin promoter driven-Cre mice (see Stock No. 003574 for example) resulted in hemizygous mice that exhibit cavernous hemangiomas of the liver, a rare component of the human von Hippel-Lindau (VHL) disease. This strain represents an effective tool for generating tissue specific-targeted mutants useful in studies examining VHL and tumor suppression in general.
When bred to a strain expressing Cre recombinase in the myeloid cell lineage (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 004597 | C;129S4-Ptentm1Hwu/J | Repository- Live |
| These mice possess loxP sites on either side of exon 5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. | ||
| 008081 | CBy.129(B6)-Sirt1tm1Ygu/J | Repository- Live |
| Mice homozygous for this targeted allele (SirT1co/co) are viable and fertile with a loxP-flanked neomycin cassette just upstream of, and a third loxP site just downstream of exon 4 of the targeted gene. The floxed mutation does not affect the protein expression of the targeted gene in MEF's or mammary tissue from homozygotes. When bred to mice that express Cre recombinase, the resulting offspring have exon 4 (encoding a conserved Sir2 motif) deleted in the cre-expressing tissue(s) (the donating investigator reports that they observe only one recombination event; complete removal of the neomycin cassette and exon 4, leaving a single loxp site remaining). These SirT1co/co mice may be useful in generating conditional mutations for studying the role of estrogen, insulin growth factor-1 (IGF-1), and transcription factors (including NF-kappaB) in mammary gland development, breast cancer, apoptosis, and metabolic diseases.
In an att ..... | ||
| 008040 | CBy.B6-Gt(ROSA)26Sortm1(HBEGF)Awai/J | Repository- Live |
| Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.
For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which ..... | ||
| 006405 | FVB-Tg(Ckmm-cre)5Khn/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice have the Cre recombinase gene driven by the muscle creatine kinase (MCK or Ckm) promoter. Cre activity is observed in skeletal and cardiac muscle. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in skeletal and cardiac muscle deletion of the flanked genome. | ||
| 006774 | FVB-Tg(Col2a1-cre/ESR1)KA3Smac/J | Repository- Live |
| Mice hemizygous or homozygous for the Col2CreERT transgene are viable and fertile. Mice from this founder line (line K from founder mouse A3) have strong tamoxifen-inducible cre expression directed to cells of the chondrogenic lineage (cartilage), with minimal (<0.1%) cre activity in the absence of tamoxifen. The CreERT protein consists of Cre recombinase fused to a mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligand 4-hydroxytamoxifen. Restricted to the cytoplasm, CreERT can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Col2CreERT mice are bred with mice containing a loxP-flanked seq> ..... For more information please see the full phenotype on the strain data sheet | ||
| 006954 | FVB-Tg(Ddx4-cre)1Dcas/J | Repository- Live |
| Mice hemizygous for this Vasa-Cre transgene are viable and fertile. Transgenic cre activity is directed to male and female germ cells starting at embryonic day (e)15-e18. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequence. In such breedings, occasional hemizygous mice may exhibit variegated cre expression in skin epithelium or global cre expression (<20% incidence). Differential parent-of-origin transgene expression is observed. When the mother harbors Vasa-Cre, virtually all progeny undergo global Cre-mediated recombination, even those that do not inherit the transgene (which may be useful in converting a "floxed" allele to a null while obviating the need to perform additional crosses to remove the transgene). To achieve germ-line specific Cre-mediated recombination in offspring, paternal Vasa-Cre mice should be used. These Vasa-Cre mice may be useful in generating c> ..... For more information please see the full phenotype on the strain data sheet | ||
| 004600 | FVB-Tg(GFAP-cre)25Mes/J | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Mice that are homozygous for the transgene are not viable. This transgenic mouse strain expresses Cre recombinase under the control of the human glial fibrillary acidic protein promoter (GFAP). When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons. Expression activity is also present in periportal cells of the liver. Developmental onset of transgene expression occurs in the dorsal and medial regions of the telencephalon by embryonic day 13.5. In adult cerebellum, only astrocytes are immunoreactive for GFAP or Cre recombinase. This mutant mouse strain represents an effective tool for generating central ner ..... For more information please see the full phenotype on the strain data sheet | ||
| 006364 | FVB-Tg(Nr5a1-cre)2Lowl/J | Repository- Live |
| Mice hemizygous for the "Sf1-Cre" transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Transgene expression mimics the mRNA pattern of Nr5a1; with Cre activity observed in steroidogenic factor-1 (SF1)-positive neurons in the ventromedial hypothalamic nucleus (VMH) as well as pituitary, gonad, and adrenal tissue. Expression is also noted in the cerebral cortex and in a few scattered cells in the caudal brainstem of mice derived from line 2 (but not line 7). If bred with mice containing a loxP-flanked sequence of interest, tissue-specific deletion of that genome results in the offspring. Specifically, these cre-expressing mice may be useful in studies involving the hypothalamus, such as body weight homeostasis, obesity, leptin metabolism, or as a reporter strain for SF1-transcription factor activity. | ||
| 008537 | FVB-Tg(Tek-cre)2352Rwng/J | Repository- Live |
| These transgenic mice express the Cre recombinase under the control of the mouse Tek, endothelial-specific receptor tyrosine kinase (also known as Tie2), promoter. Cre recombinase expression is detected in most endothelial cells and blood islands of the extraembryonic mesoderm by embryonic day (ED)7.5 and in the dorsal aorta by ED8.5. Especially high levels of expression are seen in head vasculature by ED9. When crossed with reporter strains (expressing beta-galactosidase or alkaline phosphatase), recombination is detected in all blood vessels and some blood cells examined at ED11.5, which indicates that Cre activity occurs in early vascular progenitor cells, endothelial cells and some hematopoietic cells. Mice homozygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain represents an effective tool for generating endothelial cell specific-targeted conditional mutations. | ||
| 009427 | FVB.129S4(B6)-Gt(ROSA)26Sortm1Sor/J | Repository- Live |
| Homozygous mice are viable and fertile, with a loxP-flanked DNA STOP sequence preventing expression of the downstream lacZ gene. When crossed with a cre transgenic strain, the STOP sequence is removed and lacZ is expressed in cells/tissues where cre is expressed. These mutant mice may be used as a Cre-reporter strain; to test the tissue/cellular expression pattern of cre transgenic mice.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available. | ||
| 005125 | FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J | Repository- Live |
| These mice contain the firefly luciferase (luc) gene inserted into the Gt(ROSA)26Sor locus. Expression of the luciferase gene is blocked by a loxP-flanked STOP fragment placed between the luc sequence and the Gt(ROSA)26Sor promoter. This strain serves as a Cre reporter strain. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision is indicated by luciferase expression in Cre-expressing tissues. Mice that are heterozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. | ||
| 008244 | FVB.Cg-Tg(tetO-cre)1Jaw/J | Repository- Live |
| Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of a tetracycline-responsive promoter element (TRE; tetO). When hemizygotes are bred with another transgenic mouse expressing either reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under the control of tissue-specific promoters, Cre recombinase expression and Cre-mediated recombination in the appropriate tissues of the bitransgenic offspring, can be regulated with the tetracycline analog, doxycycline. This strain represents an effective tool for generating inducible, tissue-specific-targeted mutants to study cell lineage during development. Importation of this model was supported by the Boomer Esiason Foundation. | ||
| 008200 | FVB/N-Tg(CAG-EGFP,-ALPP)2.6Ggc/J | Repository- Live |
| Mice harboring the piGAP transgene are viable and fertile, with expression of the eGFP-F-IRES-hPLAP dicistronic gene blocked by an upstream loxP-flanked STOP-polyA sequence. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP-polyA sequence deleted in the cre-expressing tissue(s), permitting dicistronic expression of eGFP-F-IRES-hPLAP. When transgene expression is induced in neurons, human Placental Alkaline Phosphatase (PLAP or ALPP) outlines axonal and dendritic projections and can be visualized by a simple histochemical reaction in fixed cells. Likewise, in vivo fluorescence of farnesylated Enhanced Green Fluorescent Protein (eGFP-F; optimized to target expression to the cytoplasmic side of the plasma membrane) labels axons, and dendrites throughout their length. Because both proteins localize alongside the neuronal surface, concomitant detection of cell body, neurites, and presynaptic and postsynaptic sites may be o ..... For more information please see the full phenotype on the strain data sheet | ||
| 003314 | FVB/N-Tg(EIIa-cre)C5379Lmgd/J | Repository- Live |
| This line carries a Cre transgene under the control of the adenovirus EIIa promoter that targets expression of Cre recombinase to the early mouse embryo. Cre expression is thought to occur prior to implantation in the uterine wall. A mosaic pattern of expression is commonly observed. Cre-mediated recombination occurs in a wide range of tissues, including the germ cells that transmit the genetic alteration to progeny.
View cre expression characterization. | ||
| 008197 | FVB/N-Tg(HTT*97Q)IXwy/J | Repository- Live |
| Mice hemizygous for the BACHD transgene are viable and fertile. Under the control of endogenous human htt regulatory machinery, BACHD mice have relatively high expression levels of a neuropathogenic, full-length human mutant Huntingtin (fl-mhtt) modified to harbor a loxP-flanked human mutant htt exon 1 sequence (containing 97 mixed CAA-CAG repeats encoding a continuous polyglutamine (polyQ) stretch). Prior to Cre recombinase exposure, BACHD mice exhibit progressive motor deficits, neuronal synaptic dysfunction, and selective late-onset neuropathology without somatic polyQ repeat instability in the aged brain. Moreover, BACHD mice reproduce a mhtt aggregation pattern reminiscent of that in adult-onset Huntington's disease (HD). Importantly, a relatively steady-state level of predominantly fl-mhtt and a small amount of mhtt N-terminal fragments present in both the nucleus and cytoplasm, are responsible for the onset and progression of neuropathology. Upon exposure to ..... For more information please see the full phenotype on the strain data sheet | ||
| 006143 | FVB/N-Tg(Thy1-cre)1Vln/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is observed in nearly all neurons in cortex and hippocampus. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in postnatal, neuron-specific deletion of the flanked genome. These mice may be useful in studies of the nervous system, including Alzheimer's disease.
View cre expression characterization. | ||
| 008694 | NOD/ShiLt-Tg(Foxp3-EGFP/cre)1Jbs/J | Repository- Live |
| Mice hemizygous for the Foxp3-EGFP/cre BAC transgene are viable and fertile. They express an enhanced green florescent protein and codon optimized "humanized" cre under the control of the mouse Foxp3 promoter. Transgene expression is specific to Cd4+Cd25 highCd127 low T cells from the lymph nodes, spleen and thymus. GFP expression is restricted to FoxP3+ cells. Ninety-five percent of the GFP+ cells in the lymph nodes and spleen display specific and efficient reporter activity when mated with a floxed allele. This mutant mouse strain may be useful for fluorescently labeling Foxp3+ T-cells to study regulatory T-cell function in autoimmunity specifically, type 1 diabetes. | ||
| 008008 | SJL.129-Cd44tm1Ugu/J | Repository- Live |
| Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice possess loxP sites on either side of exon 6 of the targeted gene. RT-PCR analysis reveals that exon 7 is not expressed in the floxed mice, and Southern blots confirm that 1.6kb of exon 7 was deleted during homologous recombination. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 6 and exon 7 deleted in the cre-expressing tissue(s). According to the donating investigator, homozygotes exhibit reduced incidence and severity of induced experimental autoimmune encephalomyelitis (EAE) when compared to wildtype controls. This mutant mouse strain may be useful in studies of multiple sclerosis (MS) and inflammatory diseases.
NOTE: Stock No. 008008 mice are on a unique "SJL/R" genetic background; the "SJL/R" strain was maintained by the Erasmus Medica ..... | ||
| 008882 | STOCK Bcl2tm1Irt/J | Repository- Live |
| Mice homozygous for this Bcl2flox conditional allele are viable and fertile, with a loxP-flanked neo cassette upstream of exon 2, as well as a loxP site downstream of exon 2 of the Bcl2 (B-cell leukemia/lymphoma 2) gene. When bred to mice that express Cre recombinase, the resulting offspring can have one of three resulting genotypes in the cre-expressing tissue(s); only the neo selection cassette deleted, only exon 2 deleted, or both the neo selection cassette and exon 2 deleted. The two latter genotypes result in loss of Bcl2 protein expression and are reported to confer the null phenotype. These Bcl2flox mutant mice may be useful in generating conditional mutations for studying apoptosis, mitochondrial permeability, cell survival signaling, cancer, neurological disorders, and immunity.
For example, when crossed to a strain expressing Cre recombinase in myeloid cell lineages (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 004339 | STOCK Bdnftm3Jae/J | Repository- Live |
| These mice possess loxP sites on either side of exon 5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
When bred to a strain expressing Cre recombinase under the control of a tetracycline-responsive promoter element (see Stock No. 006224, 006234, 006244) and a strain expressing a tetracycline-controlled activator protein in brain tissues (see Stock No. 003763), this mutant mouse strain may be useful in studies of hippocampal-dependent learning and long-term potentiation. When bred to a strain expressing Cre recombinase in neuronal cells (see Stock No. 003966 for example), this ..... | ||
| 008670 | STOCK Blmtm4Ches/J | Repository- Live |
| These mice possess loxP sites on either side of exon 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 8 deleted in the cre-expressing tissue(s).
When bred to a strain with Cre recombinase expression in mammary cells, or a strain with widespread Cre recombinase expression, this mutant mouse strain may be useful in studies of Bloom Syndrome. | ||
| 006001 | STOCK Dicer1tm1Bdh/J | Repository- Live |
| These mice contain loxP sites on either side of exon 23 of the targeted gene. Mice homozygous for this "Dicer-flox" allele are viable and fertile and exhibit no gross phenotypic or behavioral abnormalities. Expression of the targeted allele is indistinguishable from wildtype despite the presence of an frt-flanked neomycin cassette between exon 23 and the 3' loxP site. Cre-mediated recombination (resulting in deletion of exon 23) in the germline leads to developmental arrest at embryonic day 7.5 (E7.5). Tissue specific deletion results in the loss of microRNA (miRNA) processing. Mutant mice can be used to generate cell/tissue-specific deletions of the endogenous gene for applications in embryonic development, translation, protein processing and miRNA/siRNA regulation of gene expression.
For example, when crossed to a strain expressing Cre recombinase in mesenchyme (see Stock No. 005584), this mutant mouse s ..... | ||
| 009063 | STOCK Ednrbtm1Nrd/J | Repository- Live |
| Mice heterozygous for this Ednrbflex3 allele are viable and fertile, with a loxP-flanked neo cassette upstream of exon 3, as well as a loxP site downstream of exon 3 of the Ednrb (endothelin receptor type B or ET-B receptor) gene. When bred to mice that express Cre recombinase, the resulting offspring can have one of three resulting genotypes in the cre-expressing tissue(s); only the neo selection cassette deleted, only exon 3 deleted, or both the neo selection cassette and exon 3 deleted. The two latter genotypes are expected to result in a frameshifted transcript that is reported to confer the null phenotype. These mutant mice may be useful in generating conditional mutations for studying the role of Ednrb in development of melanocytes, development of neurons and glia of the enteric nervous system, neural crest-derived cells, mesenchymal-derived smooth muscle cells, vasodilation, mitogen signaling and cancer, and human Hirschsprung's dis ..... For more information please see the full phenotype on the strain data sheet | ||
| 009672 | STOCK Egln1tm1Kael/J | Repository- Live |
| These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene (widespread deletion of expression is embryonic lethal). This strain may be useful in studies of development and cardiovascular disease.
For example, when crossed to a strain expressing tamoxifen-inducible Cre recombinase in most tissues (see Stock No. 004682), this mutant mouse strain may be useful in studies of polycythemia and congestive heart failure. | ||
| 007916 | STOCK En1tm2(cre)Wrst/J | Repository- Live |
| En1Cki (or En1Cre) mutant mice harbor a Cre recombinase (cre) "knock-in" allele that also abolishes endogenous gene function. While heterozygotes are viable and fertile, homozygous mice die at birth. Under control of the upstream promoter/enhancer elements, cre expression is observed in a pattern consistent with the wild-type gene. When heterozygotes are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequences in En1-expressing tissues of the offspring. These En1Cki (or En1Cre) mice may be useful for Cre-lox applications studying engrailed protein function such as deleting genes in spinal cord V1 interneurons, the embryonic mesencephalon and rhombomere 1 by E9, as well as in the ventral ectoderm of the limbs, in a subset of somite cells, and some mesoderm-derived tissues.
Of note, these mice may also be useful in conjunction with o ..... | ||
| 007917 | STOCK En1tm7(cre/ESR1)Alj/J | Repository- Live |
| While mice heterozygous for this En1-CreERT1 mutation are viable and fertile, homozygotes die at birth. Because the Cre-ERT1 fusion gene is inserted between the transcriptional start site and the first exon of the engrailed 1 (En1) gene, tamoxifen-inducible cre activity is controlled by the endogenous En1 regulatory elements. The Cre-ERT1 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT1 can only gain access to the nuclear compartment after exposure to OHT. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these En1-CreERT1 mice are bred with ..... For more information please see the full phenotype on the strain data sheet | ||
| 007918 | STOCK En1tm8.1Alj/J | Repository- Live |
| Mice homozygous for the En1flox conditional allele are viable and fertile, with loxP sites flanking the coding region of exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (encoding the engrailed homeodomain) deleted in the cre-expressing tissue(s). These En1flox mice may be useful in generating conditional mutations for studying engrailed protein function in the embryonic mesencephalon and rhombomere 1, as well as developing cerebellum, limbs, somites, and skin. Of note, these mice may also be useful in conjunction with other engrailed mutants (such as Stock No. 007912, Stock No. 007916, Stock No. 007917, Stock No. 007924, and Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 008407 | STOCK Epas1tm1Mcs/J | Repository- Live |
| These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon deleted in the cre-expressing tissue(s).
For example, when bred to a strain with inducible Cre recombinase expression in cardiac cells (see Stock No. 005657 for example), this mutant mouse strain may be useful in studies of erythropoiesis. When bred to a strain with tamoxifen inducible widespread Cre recombinase expression(see Stock No. 008085 for example), this mutant mouse strain may be useful in studies of erythropoiesis. | ||
| 007569 | STOCK Fgfr2tm1Dor/J | Repository- Live |
| Mice homozygous for this Fgfr2flox allele possess loxP sites flanking exons 8-10 of the targeted gene and are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have sequences encoding the alternatively spliced Ig domain IIIb, as well as the IIIc and TM domains, deleted in the cre-expressing tissue(s). These Fgfr2-flox mutant mice may be useful in generating conditional mutations to study the role of fibroblast growth factor receptors in vertebrate development; including early embryogenesis, regional specification of the brain, limb morphogenesis, and normal bone, craniofacial, and lens development.
For example, when crossed to a strain expressing Cre recombinase in the central nervous system, especially astrocytes (see Stock No. 004600), this mutant mouse strain may be useful in studies of astroglial migration. | ||
| 008464 | STOCK Foxa2tm2.1(cre/Esr1)Moon/J | Repository- Live |
| This strain expresses the tamoxifen-inducible cre/Esr1 from the targeted locus. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions specifically in the developing endoderm, notochord, and floorplate. Heterozygotes and homozygotes are normal in size, viability and fertility. | ||
| 008194 | STOCK Gata4tm1.1Sad/J | Repository- Live |
| Mice homozygous for this Gata4loxP conditional allele are viable and fertile, with loxP sites flanking exons 3-5 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for both zinc finger DNA-binding domains and the nuclear localization signal essential for GATA4 function) deleted in the cre-expressing tissue(s). These GATA binding protein 4 conditional mice may be useful in generating conditional mutations for studying GATA4 function during organogenesis (such as cardiogenesis) or in adult mice.
For example, when crossed to a strain expressing Cre recombinase in cardiac myocytes (see Stock No. 009074), this mutant mouse strain may be useful in studies of cardiac hypertrophy, stress-compensation and myocyte viability. | ||
| 008196 | STOCK Gata6tm2.1Sad/J | Repository- Live |
| Mice homozygous for this Gata6loxp conditional allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for the majority of the GATA6 protein) deleted in the cre-expressing tissue(s). These Gata6loxp mice may be useful in generating conditional GATA binding protein 6 mutations for studying GATA6 function in organogenesis or in adult mice.
For example, when bred to a strain expressing Cre recombinase in the villi and crypt cells of the intestine (see Stock No. 004586 for example), or when bred to a strain expressing Cre recombinase in the embryo (see Stock No. 003724, 003314 for example), this mutant mouse strain may be useful in studies of the transcrip ..... | ||
| 007927 | STOCK Gbx2tm1.1Alj/J | Repository- Live |
| Mice homozygous for the Gbx2flox conditional allele are viable and fertile, with loxP sites flanking the protein coding region of exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (encoding the gastrulation brain homeobox 2 homeodomain) deleted in the cre-expressing tissue(s). These Gbx2flox mice may be useful in generating conditional mutations for studying gastrulation brain homeobox protein function in the embryonic mesencephalon and rhombomere 1, as well as developing cerebellum and thallamus. | ||
| 007913 | STOCK Gli1tm3(cre/ESR1)Alj/J | Repository- Live |
| Mice homozygous for this Gli1-CreERT2 targeted allele are viable and fertile (although homozygous males are reported to have breeding problems). Under control of the endogenous upstream promoter/enhancer elements, tamoxifen-inducible cre activity is observed in cells that have received positive Hedgehog/Sonic Hedgehog signaling. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Gli1-CreERT2 mice are ..... For more information please see the full phenotype on the strain data sheet | ||
| 007926 | STOCK Gli2tm6Alj/J | Repository- Live |
| Mice homozygous for this Gli2flox conditional allele are viable and fertile, with loxP sites flanking exons 7 and 8 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have these exons deleted in the cre-expressing tissue(s). This results in a frameshift mutation following splicing of mRNA from exon 6 to 9 and is reported to confer the null phenotype. These Gli2flox mutant mice may be useful in generating conditional mutations for studying Hedgehog/Sonic Hedgehog signaling in the development of many organs (such as central nervous system and axis patterning), as well as the role of Gli2 in adult organs. | ||
| 006331 | STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J | Repository- Live |
| Homozygous "ROSA26-eGFP-DTA" mice are viable, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator reports that some homozygous males are subfertile. Mutant mice display widespread expression of EGFP, but DTA transcription is prevented by a strong transcriptional stop sequence. When bred to mice that express Cre recombinase under the control of a promoter of interest, the loxP-flanked EGFP and stop sequence are removed, and DTA expression is activated, resulting in the specific ablation of cre-expressing cells. This strain may be useful on its own as a fluorescent reporter or in combination with cre-expressing mice to produce conditional deletions of specific groups of cells. Transgenic mice also may have applications in toxicology and protein synthesis research.
For example, when crossed to a strain expressing Cre recombinase in the midbrain and dorsal spinal cord (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 008159 | STOCK Gt(ROSA)26Sortm1(Notch1)Dam/J | Repository- Live |
| These mice contain a sequence encoding an intracellular portion of the mouse Notch1 gene (amino acids 1749-2293), but lacking the c-terminal PEST domain, and Green Fluorescent Protein, GFP, inserted into the GT(ROSA)26Sor locus. Expression of the Notch1 fragment and GFP is blocked by a loxP-flanked STOP fragment placed between the coding sequence and the GT(ROSA)26Sor promoter. The GFP expression is localized to the nucleus by an IRES sequence. The truncated cytoplasmic fragment encoded by the Notch1 sequence causes constitutive signaling activity. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants for studying the effects of Notch pathway activation. Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
For example, when crossed to a strain expressing a tamoxifen inducible Cre recombinase in all c ..... | ||
| 005130 | STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J | Repository- Live |
| These mice contain an Enhanced Yellow Fluorescent Protein/Smoothened homolog (Drosophila) fusion gene inserted into the Gt(ROSA)26Sor locus. The mutant allele consists of a fusion product involving Enhanced Yellow Fluorescent Protein (EYFP) and the constitutively active W539L point mutation of the mouse smoothened homolog (Drosophila) gene (SmoM2). Expression of the Smo/EYFP fusion gene is blocked by a loxP-flanked STOP fragment placed between the Gt(ROSA)26Sor promoter and the Smo/EYFP sequence. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision results in the constitutive expression of mouse smoothened homolog (Drosophila) and unrestrained Hedgehog signaling in Cre-expressing tissues. Expression of the SmoM2 fusion protein can be monitored using EYFP-specific fluorescence protocols. Mice that are homozygous for the mutant allele are viable, fertile, normal in size and do not display any gross physical ..... For more information please see the full phenotype on the strain data sheet | ||
| 005572 | STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J | Repository- Live |
| Homozygous mutant mice are viable, fertile, normal in size and do not display any behavioral abnormalities. When these gene targeted mice are bred to transgenic strains expressing Cre recombinase, functional rtTA and EGFP activity is observed in the double mutant offspring in the tissues that express cre. These double mutant mice may be bred to transgenic strains carrying genes of interest under the regulation of tetracycline responsive elements (TRE; tetO) to generate triple mutant mice in which the tissue specificity of the Cre-transgenic line and the doxycycline inducibility of the rtTA/TRE-controlled transgenes can be combined to regulate expression of the target gene. Of note, mutant mice are also available on a C57BL/6J genetic background (see Stock No. 005670). | ||
| 008600 | STOCK Gt(ROSA)26Sortm1(tTA)Roos/J | Repository- Live |
| Mice heterozygous for the ROSA:LNL:tTA conditional mutation of the Gt(ROSA)26Sor locus are viable and fertile, with a loxP-flanked STOP cassette preventing transcription of a downstream optimized/modified tetracycline-controlled transactivator protein ("mtTA"). Applying both Cre-lox and Tet-Off technologies, these ROSA:LNL:tTA mutant mice may be useful to generate compound mutant mice in which expression of a tetracycline-responsive promoter element (TRE; also called tet-operator or tetO)-driven gene of interest can be both directed to the cell types defined by the chosen Cre recombinase expression, as well as turned off by the addition of tetracycline (or its analog doxycycline (dox)). | ||
| 007576 | STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J | Repository- Live |
| Mice homozygous for this mT/mG mutation are viable and fertile. These mice possess loxP sites on either side of a membrane-targeted tdTomato (mT) cassette and express strong red fluorescence in all tissues and cell types examined. Tail or whole body epifluorescence is sufficient to identify mT/mG homozygotes. When bred to Cre recombinase expressing mice, the resulting offspring have the mT cassette deleted in the cre expressing tissue(s), allowing expression of the membrane-targeted EGFP (mG) cassette located just downstream. The donating investigator reports that the ACTB promoter allows stronger and persistent expression of the fluorescent proteins (especially in adult cells) compared to the endogenous Gt(ROSA) locus alone. This double-fluorescent system allows direct live visualization of both recombined and non-recombined cells at single cell resolution, offering an internal control for phenotypic analysis of Cre-induced mosaic mutants and providing a second marker fo ..... For more information please see the full phenotype on the strain data sheet | ||
| 008458 | STOCK Mir17-92tm1.1Tyj/J | Repository- Live |
| The miR-17~92 (Mir17, Mir18, Mir19a, Mir20a, Mir19b-1, Mir92-1) cluster, overexpressed in human cancers, is flanked by loxP sites in this targeted mutation strain. Mice homozygous for the "floxed" allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. | ||
| 006951 | STOCK Notch1tm2Rko/GridJ | Repository- Live |
| Mice homozygous for this "floxed" Notch1 allele (fN1) are viable and fertile. In the targeted allele, loxP sites were placed flanking exon 1 of the targeted gene. When these floxed mice are bred to mice expressing Cre recombinase, exon 1 of the targeted gene is deleted in cre-expressing tissue(s) in the cre-positive, homozygous floxed offspring. These conditional knockout mice may be useful in generating tissue-specific mutants for studying the development of a wide range of tissues: for example, when crossed to a strain expressing Cre recombinase primarily in the nervous system (see Stock No. 003771), this mutant strain may be useful in studies of apoptosis in neural development. | ||
| 006953 | STOCK Notch1tm3(cre)Rko/J | Repository- Live |
| While homozygotes die at embryonic day 9.5 (E9.5), mice heterozygous for this N1::cre allele are viable and fertile. Mutant mice express a Notch1-Cre fusion protein formed by the insertion of Cre recombinase into the Notch1 locus replacing the intracellular domain (NICD1). As such, endogenous function of the NICD1 is terminated. Interaction of N1::cre fusion receptors in vivo with Notch-DSL (-Delta, Serrate and Lag-2) ligands results in proteolytic cleavage of the transmembrane domain tether and subsequent release of Cre recombinase protein from the plasma membrane. When bred to mice with a loxP-flanked reporter sequence, cell descendants have differential Cre-mediated reporter protein labeling; Notch1 signaling in actively cycling stem/progenitor cells will mark all their descendants producing a "clone", whereas Notch1 activation in transit amplifying or differentiating cells will result in small clones (2-4 cells) or in salt-and-pepper patterns of individually la ..... For more information please see the full phenotype on the strain data sheet | ||
| 005384 | STOCK Numbtm1Zili Numbltm1Zili/J | Repository- Live |
| These mice possess loxP sites on either side of exon 1 of the Numb gene and loxP sites flanking exons 1 through 3 of the Numbl gene. Mice that are homozygous for these alleles are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.
For example, when crossed to a strain expressing an interferon inducible Cre recombinase (see Stock No. 003556), this mutant mouse strain provides information about hemopoiesis and lymphopoesis. | ||
| 004526 | STOCK Smotm2Amc/J | Repository- Live |
| These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to a strain with the targeted null allele (Stock No. 004288) and a strain expressing Cre recombinase in the skin (Stock No. 004782), this mutant mouse strain may be useful in studies of hedgehog signalling and cell proliferation in the dental epithelium. When bred to a strain with the targeted null allele (Stock No. 004288) and a strain expressing Cre recombinase in the nervous system (Stock No. 003771), this mutant mouse strain may be useful in studies of hedgehog signalling and cerebellar foliation. When bred to ..... | ||
| 005680 | STOCK Tsc1tm1Djk/J | Repository- Live |
| Homozygous mice are viable, fertile, normal in size, have normal expression of hamartin (the targeted gene's protein product), have no growth or behavioral defects, and are devoid of tumors through age 18 months. This mutant carries a "floxed" allele of the endogenous gene. When combined with a mutant carrying a Cre recombinase gene under the control of a promoter of interest, exons 17 and 18 of Tsc1 are deleted in the tissue of interest. This mutant may be useful in many tissue-specific studies including tuberous sclerosis or other hamartoma syndromes, regulation of the actin cytoskeleton and motility, cellular and organismal glucose homeostasis, cell growth responses, apoptosis regulation, and regulation of cell size.
When bred to a strain expressing Cre recombinase in neuronal cells (see Stock No. 003966 for example), this mutant mouse strain may be useful in studies of tuberous sclerosis.
Importatio ..... | ||
| 008469 | STOCK Wnt9btm1.2Amc/J | Repository- Live |
| Mice homozygous for the Wnt9bc allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). Such deletion is predicted to result in out-of-frame splicing of exon 1 to exon 3, and consequently a mutant transcript that would encode a nonfunctional peptide comprising the first 27 amino acids of Wnt9b that includes the signal peptide. These Wnt9bc mice may be useful in generating conditional mutations for studying the role of Wnt9b (and other Wnt family members) in development and canonical Wnt signaling cascades, including metanephric kidney and urogenital system development. | ||
| 007684 | STOCK Tg(Atoh1-cre/ESR1)14Fsh/J | Repository- Live |
| Mice hemizygous for this Math1-CreERT2 transgene are viable and fertile. Under control of the Math1 (Atoh1) enhancer, tamoxifen-inducible cre activity is observed in neural progenitors of the cerebellar rhombic lip, dorsal hindbrain and spinal cord, as well as in inner-ear primordia (with a limited amount of ectopic expression in the primordium of the hippocampus but not the cortex). The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone ma ..... For more information please see the full phenotype on the strain data sheet | ||
| 006882 | STOCK Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe/J | Repository- Live |
| Mice hemizygous for this "Z/AP-AML1-ETO" transgene (coding for the translocation t(8;21) present in 15% of acute myeloid leukemias (AML)) are viable and fertile. Homozygotes die in utero presumably due to high lacZ expression. Prior to cre-mediated excision of the "floxed" STOP sequence, expression of lacZ is observed in all tissues including bone marrow progenitor cells. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the human AML1-ETO fusion protein and placental alkaline phosphatase (ALPP or PLAP) to proceed in the Cre recombinase expressing cells. While pan expression of AML1-ETO leads to embryonic lethality (E7.5), hematopoietic and endothelial expression leads to malignancy in B- and T- lymphoid cells and secondary mutations that closely resemble the association of AML1-ETO with acute myeloid leukemia in humans. These transgenic mice may ..... For more information please see the full phenotype on the strain data sheet | ||
| 005438 | STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J | Repository- Live |
| While mice hemizygous for this Z/RED transgene are reported to be viable and fertile, it has been our experience at The Jackson Laboratory that hemizygous animals are often smaller than littermates and subject to postnatal mortality. Delayed weaning greatly enhances the survival. Although homozygous animals are born, animals have not survived past five weeks of age. These transgenic mice express beta-galactosidase under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with red fluorescent protein (DsRed*MST) expression in tissues expressing Cre recombinase. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells. | ||
| 006850 | STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J | Repository- Live |
| Mice hemizygous for this Cre-conditional IC-Notch (or Z/EG-Notch) transgene are viable and fertile. Homozygotes die in utero, presumably due to high lacZ expression. Prior to Cre-mediated excision of the "floxed" STOP sequence, high expression of lacZ is observed in cells and tissues. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the intracellular human NOTCH1 (IC-Notch) and EGFP to proceed in the Cre recombinase expressing cells. For example, endothelial expression of IC-Notch using different Cre-transgenic matings is associated with neural, somite and angiogenic defects, infertility in females, and embryonic lethality in the resulting offspring. These transgenic mice may be useful for global expression of lacZ or, when crossed to a Cre recombinase expressing strain, for studying the role of Notch signaling during both embryonic develo ..... For more information please see the full phenotype on the strain data sheet | ||
| 003920 | STOCK Tg(CAG-Bgeo/GFP)21Lbe/J | Repository- Live |
| These Z/EG transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being liver and lung tissue. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with enhanced GFP expression in tissues expressing cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess cre excision activity in live animals and cells. Although homozygotes are viable, attempts to breed homozygous mice proved unsuccessful. | ||
| 008861 | STOCK Tg(Ela1-Cre/ESR1)1Stof/J | Repository- Live |
| These transgenic mice have a tamoxifen-inducible, Cre-mediated recombination system driven by the rat elastase 1, pancreatic (Ela1) promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the human estrogen receptor ligand binding domain (CreERT2). The mutant human estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/ESR1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. No cre activity is detectable without tamoxifen treatment. When these transgenic mice are bred with mice containing a loxP flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Ela1
expressing cells; making them useful in fate mapping of pancreatic acinar cells.
Hemizygous mutant mice are viable, fertile, normal in ..... For more information please see the full phenotype on the strain data sheet | ||
| 005938 | STOCK Tg(Eno2-cre)39Jme/J | Repository- Live |
| Mice hemizygous for this NSE39-Cre transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These NSE39-Cre mice harbor a transgenic insert consisting of the cre recombinase gene under the control of the promoter region of the rat neuron specific enolase (NSE or Eno2) gene. As such, Cre recombinase activity is directed to neurons with expression in many tissue types. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome.
Specifically, these NSE39-Cre transgenic mice may also be useful in studies of spinal muscular atrophy (SMA) along with mice harboring a conditional (floxed) Smn1 gene (see Stock No. 006138 or Stock No. 006146).
Additional SMA strains expressing cre in striated muscle are av ..... For more information please see the full phenotype on the strain data sheet | ||
| 004782 | STOCK Tg(KRT14-cre)1Amc/J | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the Cre recombinase under the control of the human keratin 14 promoter. Cre transcript is detected in the skin. When crossed to a reporter line containing Gt(ROSA)26Sortm1Sor, Beta-galactosidase activity is detected in the oral ectoderm at 11.75 dpc, and at 14.5 dpc activity is detected in the skin and throughout the dental epithelium. This strain represents an effective tool for generating tissue-specific targeted mutants that would be useful to study developmentally critical gene function in the ectoderm and its derivatives.
View cre expression characterization. | ||
| 005107 | STOCK Tg(KRT14-cre/Esr1)20Efu/J | Repository- Live |
| These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the human keratin 14 (KRT14) promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen (tamoxifen). Restricted to the cytoplasm, the cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted keratinocyte-specific deletions. Oral tamoxifen administration induces Cre recombination in toe, back skin and tongue. Topically administered tamoxifen induces Cre-mediated recombination in a specific localized area of the skin, occuring in 50 to 60% of the ..... For more information please see the full phenotype on the strain data sheet | ||
| 003553 | STOCK Tg(MMTV-cre)4Mam/J | Repository- Live |
| This transgenic strain expresses P1 Cre recombinase under the control of the MMTV LTR promoter. The MMTV LTR promoter directs a widespread pattern of expression. High levels of recombination have been detected in the virgin and lactating mammary gland, salivary gland, seminal vesicle, skin, erythrocytes, B and T cells. Little background recombination was observed in the lung, kidney, liver and brain tissues (less than 10%). The donating investigator indicates that this strain may be suitable for use in applications where it is desirable to delete genes in the virgin and lactating mammary gland, skin, erythroid cells, and other secretory tissues and skin. | ||
| 009074 | STOCK Tg(Myh6-cre)1Jmk/J | Repository- Live |
| These transgenic mice express nuclear-localizing Cre recombinase under the control of the mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha; alpha-MHC) promoter. Cre recombinase expression is detected in a majority of cardiac myocytes. Approximately 70% efficiency has been demonstrated when crossed with one floxed allele strain. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. | ||
| 008119 | STOCK Tg(Neurog3-cre/Esr1)1Dam/J | Repository- Live |
| These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse neurogenin 3, Neurog3, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in islet cells of the pancreas, undifferentiated spermatogonia and other Neurog3 expressing cells. Transgene expression closely patterns endogenous gene expression as analyzed by in situ hybridiza ..... For more information please see the full phenotype on the strain data sheet | ||
| 006207 | STOCK Tg(Pcp2-cre)1Amc/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is observed in parasagittal domains of the cerebellum beginning at embryonic day 17 (E17). At E19, low level expression is observed in the most rostral lobe of cerebellum and expression broadens until all Purkinje cells express the transgene in adult mice. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome. These transgenic mice may be useful in studies utilizing "Cre-lox" technology, specifically regarding the nervous system, development and patterning of cerebellum, and cerebellar hypotrophy converse of Lhermitte-Duclos. Important Note: These mice also carry a GFP transgene that co-integrated with the Tg(Pcp2-cre)1Amc transgene. As such, GFP expression is reported in the retina. | ||
| 005965 | STOCK Tg(Pomc1-cre)16Lowl/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is demonstrable in brain area neurons involved in the control of food intake (arcuate nucleus (hypothalamus) and solitary tract nucleus (hindbrain)). When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked genome in tissues that normally express Pomc1. The mice may be useful in studies of obesity, food intake, hunger, endocrine and exocrine function, and for tissue specific gene targeting. | ||
| 004783 | STOCK Tg(Sox2-cre)1Amc/J | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse SRY-box containing gene 2 promoter. When crossed to a reporter line, Beta-galactosidase activity is detected in the epiblast cells at embryonic day 6.5, with little or no activity in other cells at gastrulation. Some activity is also detected in extra embryonic derivatives of the epiblast, the yolk sac mesoderm and amnion. No activity is detected in primitive endoderm derived tissues, visceral endoderm. This strain represents an effective tool for generating epiblast-derived specific targeted mutants. | ||
| 008208 | STOCK Tg(Stra8-cre)1Reb/J | Repository- Live |
| Homozygous Stra8-cre transgenic mice are viable and fertile, with expression of an optimized variant of Cre recombinase (iCre) directed by the 1.4 kb mouse Stra8 (stimulated by retinoic acid gene 8) genomic promoter fragment. Postnatal Cre-recombinase expression is first observed in testes at postnatal day (P)3 (early-stage spermatogonia), increases out to P7 (pre-leptotene-stage spermatocytes), and is not detected in other tissues examined (including ovaries). In addition, no cre expression is reported in male or female embryos. When Stra8-cre transgenic males are bred with female mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence, specifically during these stages of spermatogenesis. These Stra8-cre transgenic mice may be useful in generating conditional knockouts in postnatal, premeiotic, male germ cells for studying spermatogenesis. | ||
| 004746 | STOCK Tg(Tagln-cre)1Her/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse transgelin (smooth muscle protein 22-alpha) promoter. Cre recombinase expression (mRNA) closely patterns endogenous transgelin expression, with the highest levels detected in the aorta, intestine and uterus. Low levels of transcript are detected in all other organs tested, likely reflecting the vascular smooth muscle compartments in the these tissues. Cre recombinase activity is observed in vascular smooth muscle cells of hepatic and pulmonary arteries, with no activity detected outside the vascular walls. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in vascular smooth muscle cells. This strain represents an effective tool for generating tissue specific-ta ..... For more information please see the full phenotype on the strain data sheet | ||
| 003829 | STOCK Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J | Repository- Live |
| Mice that are homozygous for both transgenic inserts are viable, fertile, normal in size and do not display any gross physical abnormalities. Both Cre recombinase and the GAL4 transcriptional activator are expressed under the direction of Wnt1 regulatory sequences. Regulated expression initially occurs in the midbrain. After neural tube closure, expression occurs in the dorsal and ventral midlines of the midbrain and caudal diencephalon, the midbrain-hindbrain junction and in the dorsal spinal cord. This versatile strain allows the simultaneous expression of Cre recombinase and GAL4 in the Wnt1 expression domain. | ||
| 007807 | STOCK Tg(Wnt1-cre)11Rth/MileJ | Repository- Live |
| These Wnt1-Cre transgenic mice express Cre recombinase under the control of the wingless-related MMTV integration site 1 (Wnt1) promoter. Cre recombinase activity is detected in the Wnt1 pattern of expression: in the midbrain by 8.5 dpc and after neural tube closure, in the dorsal and ventral midlines of the midbrain and caudal diencephalon, midbrain-hindbrain junction and dorsal spinal cord. When crossed with a strain containing loxP site-flanked sequences, Cre-mediated recombination results in deletion of the flanked sequences in the developing neural tube tissues of the resulting offspring. The donating investigator reports that homozygous mice may be lethal as some offspring from transgenic carrier parents die around two months of age.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the ..... | ||
| 008199 | STOCK Tg(dlx6a-cre)1Mekk/J | Repository- Live |
| Homozygous Dlx5/6-Cre transgenic mice are viable and fertile. Expression of Cre recombinase (Cre) is directed to differentiating and migrating forebrain GABAergic neurons during embryonic development by the I56i and I56ii enhancers from the zebrafish dlx5a/dlx6a intergenic region (with the 5' promoter region of zebrafish dlx6a in place to increase the activity of the intergenic enhancers rather than direct tissue-specific expression). When Dlx5/6-Cre transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence in the offspring in the offspring. These Dlx5/6-Cre transgenic mice may be useful in generating specific deletions of floxed alleles in GABAergic forebrain neurons. | ||
| 006224 | STOCK Tg(tetO-cre)1Jaw/J | Repository- Live |
| Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of a tetracycline-responsive promoter element (TRE; tetO). When hemizygotes are bred with another transgenic mouse expressing either reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under the control of tissue-specific promoters, Cre recombinase expression and Cre-mediated recombination in the appropriate tissues of the bitransgenic offspring, can be regulated with the tetracycline analog, doxycycline. This strain represents an effective tool for generating inducible, tissue-specific-targeted mutants to study cell lineage during development. Importation of this model was supported by the Boomer Esiason Foundation. | ||
| 005947 | B6.Cg-Tg(Wap-cre)11738Mam/JKnwPea | Research Strain |
| WAP-Cre transgenic mice are viable and fertile, with expression of P1 Cre recombinase under the control of the mouse Wap (whey acidic protein) promoter. When bred with other mutant mice containing loxP-flanked sequence(s), Cre-mediated recombination will result in deletion of the floxed sequence(s) in mammary gland epithelium. Deletion of floxed sequences is reported in brain tissue from WAP-Cre mice on a mixed genetic background but has not been examined in this strain. Maximum Cre recombinase activity is achieved during pregnancy and lactation, but in virgin mice small numbers of mammary epithelial cells show evidence of estrus cycle-related Cre recombinase activity. These WAP-Cre transgenic mice may be useful in generating conditional mutations in mammary glands for studying mammary physiology and processes such as breast development and breast cancer. | ||
| 006373 | 129-Braftm1Sva/J | Cryopreserved - Ready for recovery |
| Homozygous "floxed B-raf" (B-raff/f) mice are viable and fertile with normal B-raf protein expression. When bred to mice expressing Cre recombinase under the control of a promoter of interest, exon 12 of the targeted gene is deleted in the tissue of interest. These mutant mice may be useful in neurological studies such as Ras/Raf and MEK/ERK signaling, synaptic (neural) plasticity, learning and memory. For example, when crossed to a strain expressing Cre recombinase in nervous tissue (see Stock No. 003771), this mutant mouse strain may be useful in studies of neuron development. For example, when crossed to a strain expressing interferon inducible Cre recombinase (see Stock No. 003755), this mutant mouse strain may be useful in studies of extraembryonic mammmalian development. | ||
| 006835 | 129-Dag1tm2Kcam/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express cre recombinase, resulting offspring can have exon 2 of the targeted gene deleted in the cre-expressing tissue(s). As the targeted gene has three loxP sites, other genotypes may also result. These mutant mice may be useful in studying muscle disease and regeneration. When bred to a strain expressing Cre recombinase under the control of the human glial fibrillary acidic protein promoter (GFAP) (see Stock No. 004600 for example), this mutant mouse strain may be useful in studies of brain abnormalities observed in congenital muscular dystrophies. | ||
| 006053 | 129-Gt(ROSA)26Sortm1Luo/J | Cryopreserved - Ready for recovery |
| MADM-GG mice are viable with no gross behavioral or observable abnormalities. Regardless of Cre-recombination, these mice express EGFP as their N- and C-terminal coding sequences are interrupted by the beta-actin intron in-frame. High EGFP expression in every cell can be visualized in vivo and in fixed samples. These mutant mice are a control EGFP-expressing strain for use with MADM (mosaic analysis with double markers) mice (see Stock No. 006041 [MADM-GR (EGFP/Dsred2)] and Stock No. 006067 [MADM-RG (Dsred2/EGFP)]). Using the MADM system, a researcher can generate genetic mosaics in which an individual organism contains somatic cells of different genotypes. This allows the researcher to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation and mitosis.
Mice harbor ..... | ||
| 006067 | 129-Gt(ROSA)26Sortm2Luo/J | Cryopreserved - Ready for recovery |
| MADM-RG mice are viable with no gross behavioral or observable abnormalities. Homozygous females produce less pups to weaning age compared to heterozygotes. These mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to mice harboring a reciprocal mutation at the same locus (see Stock No. 006041 or Stock No. 006075, MADM-GR (EGFP/Dsred2)). The resulting offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous") and must next be bred to a Cre-expressing strain for fluorescent protein expression. Prior to Cre-recombination, double mutant mice do not have colored cells: the chimeric genes do not produce functional proteins because their coding sequences are interrupted by the beta-actin intron in different reading frames. After DNA replication (G2 phase) in double mutant mice, Cre-recombinase introducti ..... For more information please see the full phenotype on the strain data sheet | ||
| 006041 | 129-Gt(ROSA)26Sortm3Luo/J | Cryopreserved - Ready for recovery |
| MADM-GR mice are viable with no gross behavioral or observable abnormalities. Homozygous mice have low fertility, while heterozygous mice have no reported fertility defects. These mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to mice harboring a reciprocal mutation at the same locus (see Stock No. 006067 or Stock No. 006080, MADM-RG (Dsred2/EGFP)). The resulting offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous") and must next be bred to a Cre-expressing strain for fluorescent protein expression. Prior to Cre-recombination, double mutant mice do not have colored cells: the chimeric genes do not produce functional proteins because their coding sequences are interrupted by the beta-actin intron in different reading frames. After DNA replication (G2 phase) in double mutant mice, Cre-recom ..... For more information please see the full phenotype on the strain data sheet | ||
| 005989 | 129;FVB-Tg(PTH-cre)4167Slib/J | Cryopreserved - Ready for recovery |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is observed only in parathyroid tissue; no activity is seen in thyroid, muscle, lymph node, trachea, thymus, salivary tissues, lung, heart, liver, brain, stomach, spleen, kidney, large intestine, small intestine, and pancreas. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in parathyroid-specific deletion of the flanked genome. These transgenic mice may be useful in generating mouse models of parathyroid-specific deletion of genes of interest, such as multiple endocrine neoplasia type 1, extracellular calcium-sensing receptor, and vitamin D receptor. | ||
| 005109 | 129S(FVB)-Men1tm1.2Ctre/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites flanking exons 3 to 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to a strain expressing Cre recombinase in parathyroid tissue (see Stock No. 005989 for example), this mutant mouse strain may be useful in studies of hypercalemia and parathyroid neoplasia. | ||
| 007664 | 129S-Efnb1tm1Sor/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites flanking exons 2 through 5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 5 deleted in the cre-expressing tissue(s). These Efnb1 conditional mutant mice may be useful in studying cellular signaling in embryonic development and adult mice; specifically receptor tyrosine kinases. For example, when crossed to a strain expressing Cre recombinase in epiblast-derived tissues (see Stock No. 003755), this mutant mouse strain may be useful in embryogenesis research. For example, when bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 007807 for example), th ..... | ||
| 003310 | 129S-Gt(ROSA)26Sortm1Sor/J | Cryopreserved - Ready for recovery |
| Mice heterozygous or homozygous for the Gtrosa26tm1Sor targeted mutation may be used to test the tissue/cellular expression pattern of the cre transgene in any transgenic strain carrying cre under the regulation of a specific promoter. Cre expression results in the removal of a loxP-flanked DNA segment that prevents expression of a lacZ gene. When crossed with a cre transgenic strain, lacZ is expressed in cells/tissues where cre is expressed. The 129-Gtrosa26tm1Sor strain is particularly useful for this purpose because the ROSA26 promoter leads to generalized expression of lacZ during development or in the adult. | ||
| 009043 | 129S-Gt(ROSA)26Sortm2Tyj/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In the absence of Cre, there is low level expression of Luciferase-SIY H2b antigen expression. After Cre activity, the recombined locus has high expression of Luciferase-SIY fusion protein. This mutant mouse strain may be useful in studies of immune response to a self-antigen or over-expressed tumor-associated antigen when used in combination with tumor-prone models. This mouse is also useful as a reporter for Cre activity. | ||
| 008002 | 129S-Pafah1b1tm2Awb/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites flanking exons 3 through 6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Histological analysis of brains from homozygotes reveals slightly wider CA1 and CA3 regions and a split in CA2 region in the hippocampus. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3 through 6 deleted in the cre-expressing tissue(s). | ||
| 008180 | 129S/Sv-Krastm4Tyj/J | Cryopreserved - Ready for recovery |
| This strain carries a point mutation (G12D) whose expression is blocked by the presence of a loxP-flanked stop codon. Homozygotes die in utero. Cre-mediated recombination can excise the stop codon and permit the oncogenic protein to be expressed. Intranasal infection with an adenovirus encoding Cre results in a very high frequency of lung tumors and permits controlled timing of tumor initiation and tumor multiplicity. This strain may be useful in studies of cancer and development.
When bred to a strain expressing Cre recombinase under the control of a tetracycline-responsive promoter element and a strain expressing a tetracycline-controlled activator protein in lung epithelial cells (see Stock No. 006234 and 006235 respectively), this mutant mouse strain may be useful in studies of lung development. When bred to a strain expressing Cre recombinase in th ..... | ||
| 004302 | 129S1/Sv-Hprttm1(cre)Mnn/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for the targeted mutation are viable and fertile. This is a Cre-deleter induced mutation strain on a 129S1 background. A Cre expression cassette was inserted into the X-linked Hprt gene. When male mice carrying a neomycin selection cassette flanked by loxP sites are mated to female mice heterozygous for this targeted mutation, the cassette is excised without detectable mosaicism, irrespective of cre inheritance. Heterozygous female mice have sufficient Cre recombinase in their oocytes to excise floxed sequence at the zygote or early cleavage stage. | ||
| 003960 | 129S6-Tg(Prnp-GFP/cre)1Blw/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Mice express the GFP/Cre fusion protein in widespread fashion. All tissues examined displayed Cre activity at an early (4-8 cell) embryonic stage. Germline expression is observed. Expression of GFP is less robust, being detectable in kidney tissue. | ||
| 007694 | B6(Cg)-Cd79btm168Mnz/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this targeted insertion are viable and fertile and do not display any gross physical or behavioral abnormalities. This strain carries a targeted insertion of an 18 bp I-SceI (S. cerevisiae) endonuclease recognition site in intron 2 of the gene. Infection of B lymphocytes with a retrovirus encoding for the I-SceI meganuclease produces Igh (immunoglobulin heavy chain )-IgB (Cd79b) chromosomal translocations with breakpoints at the I-SceI sequence on the mutant allele. | ||
| 008118 | B6(Cg)-Snord116tm1Uta/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this 2-loxP (floxed) allele are viable and fertile, with loxP sites flanking the Snord116 small nucleolar RNAs (snoRNAs) gene cluster. When bred to mice that express Cre recombinase, the resulting offspring will have this gene cluster deleted in the cre-expressing tissue(s). Because the Snord116 gene cluster is imprinted and only expressed from the paternal allele, breeding 2-loxP males with cre-expressing females may be required to generate deleted offspring with the knockout phenotype. The donating investigator reports that the distance between the two loxP sites (~140 kb) may reduce the recombination efficiency in somatic cells. As deletions of the Snord116 cluster are associated with Prader-Willi syndrome (PWS), mice carrying the 2-loxP (floxed) allele may be useful in generating conditional mutations for studying the role of Snord116 in growth and feeding regulation, mechanisms of obesity, and patho ..... For more information please see the full phenotype on the strain data sheet | ||
| 007665 | B6.129(C3)-Vcam1tm2Flv/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of the cytokine-responsive promoter region and exon 1 of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene (widespread deletion of expression is lethal).
For example, when crossed to a strain expressing interferon inducible Cre recombinase (see Stock No. 003556), this mutant mouse strain may be useful in studies of bone marrow nonhematopoietic cells. When crossed to a strain expressing Cre recombinase in endothelial cells (see Stock No. 004128), this mutant mouse strain may be useful in studies of lymphocyte migration. | ||
| 006203 | B6.129(FVB)-Ahrtm3.1Bra/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Hepatic protein expression of the conditional allele (before exon 2 excision) is equivalent to wildtype by Western blot analysis. This strain may be useful in generating tissue-specific mutants of the floxed allele for use in studies including teratogenesis and xenobiotic metabolism (including dioxin and PCB), Per-Arnt-Sim transcription factors, and fetal vascular development such as ductus venosus closure.
When bred to a strain expressing Cre recombinase in hepatocytes (see Stock No. 003574 for example), this mutant mouse strain may be useful in studies of toxicology. When bred to a strain expressing Cre recombinase in endothelial cells (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 004318 | B6.129(FVB)-Gabra1tm1Geh/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of a Gabra1 exon encoding the second transmembrane domain. Mice that are homozygous for this floxed Gabra1 allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of Gabra1. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available. Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations are available from this donating investigator (Dr. Gregg Homan ..... | ||
| 005247 | B6.129(SJL)-Nrp1tm2Ddg/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. | ||
| 006834 | B6.129-Dag1tm2Kcam/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a Cre recombinase gene under the control of a promoter of interest, exon 2 of the targeted gene and a neomycin cassette are deleted in the tissue of interest. These mutant mice may be useful in studying muscle disease and regeneration. When bred to a strain expressing Cre recombinase under the control of the human glial fibrillary acidic protein promoter (GFAP) (see Stock No. 004600 for example), this mutant mouse strain may be useful in studies of brain abnormalities observed in congenital muscular dystrophies. | ||
| 006887 | B6.129-Dpagt1tm2Jxm/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this floxed targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exon is deleted by cre expression to produce a null allele. | ||
| 006874 | B6.129-Gabra4tm1.2Geh/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this GABAA-R alpha4F allele are viable and fertile. These mutant mice have loxP sites flanking exon 3 of the targeted gene. When bred to Cre-recombinase expressing mice, offspring will have a deletion of exon 3 in the cre expressing tissue(s). These "floxed" mice may be useful in neurological studies including behavior and neurotransmitter function. Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations are available from this donating investigator (Dr. Gregg Homanics, University of Pittsburgh), including Gabra1 (Stock No. 004318), Gabra4 (Stock No. 006874), Gabra6 (Stock No. 002710), Gabrb3 (Stock No. 002711), Gabrd (Stock No. ..... | ||
| 006896 | B6.129-Galnt13tm1Jxm/J | Cryopreserved - Ready for recovery |
| Mice homozygous for the floxed targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele. This glycosyltransferase is also sometimes termed as ppGalNAcT-13 in studies of human tissues. | ||
| 006895 | B6.129-Galnt1tm1Jxm/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this floxed targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exon is deleted by cre expression to produce a null allele. | ||
| 006886 | B6.129-Gcnt1tm1Jxm/J | Cryopreserved - Ready for recovery |
| Mice homozygous for the mutation are viable, fertile and do not display any gross physical or behavioral abnormalities. The mice are devoid of significant gene-encoded enzyme activity in spleen, bone marrow, and kidney. They exhibit a moderate increase in the number of neutrophils in the blood, a partial deficiency of selectin ligands, and a mild B cell homing deficit. Neutrophil rolling is reduced on E-, L- and P-selectin substrates. | ||
| 006924 | B6.129-Gcnt3tm1Jxm/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for the floxed targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele. | ||
| 006925 | B6.129-Gcnt4tm1Jxm/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this floxed targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to Cre transgenic strains, the loxP-flanked exons are deleted by Cre expression to produce a null allele. | ||
| 006071 | B6.129-Gt(ROSA)26Sortm1Luo/J | Cryopreserved - Ready for recovery |
| Homozygous and heterozygous mutant mice are viable and fertile, and manifest no gross behavioral or phenotypic abnormalities. These mutant mice carry an EGFP construct in which the N- and C-terminal coding sequences are interrupted by the beta-actin intron in-frame. Despite the presence of this intron, high EGFP expression in every cell can be visualized in vivo and in fixed tissues.
These mutant mice were designed as an EGFP-expressing control strain for use with MADM (mosaic analysis with double markers) mice (See Stock No. 006041 [EGFP/Dsred2] and Stock No. 006067 [Dsred2/EGFP]). Using the MADM system, a researcher can generate genetic mosaics in which an individual organism contains somatic cells of different genotypes. This allows the researcher to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be ..... | ||
| 006080 | B6.129-Gt(ROSA)26Sortm2Luo/J | Cryopreserved - Ready for recovery |
| MADM-RG mice are viable with no gross behavioral or observable abnormalities. Homozygous females produce less pups to weaning age compared to heterozygotes. These mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to mice harboring a reciprocal mutation at the same locus (see Stock No. 006041 or Stock No. 006075, MADM-GR (EGFP/Dsred2)). The resulting offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous") and must next be bred to a cre-expressing strain for fluorescent protein expression. Prior to Cre recombination, double mutant mice do not have colored cells: the chimeric genes do not produce functional proteins because their coding sequences are interrupted by the beta-actin intron in different reading frames. After DNA replication (G2 phase) in double mutant mice, Cre recombinase int ..... For more information please see the full phenotype on the strain data sheet | ||
| 006891 | B6.129-Mgat1tm2Jxm/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this floxed targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to tissue-specific cre transgenic strains, the loxP-flanked exon is deleted by cre expression. | ||
| 006892 | B6.129-Mgat2tm1Jxm/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this floxed targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exon is deleted by cre expression. | ||
| 006903 | B6.129-Mgat4btm1Jxm/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for the floxed targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele. | ||
| 007003 | B6.129-Mycntm1Psk/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.
For example, when crossed to a strain expressing Cre recombinase in neuronal and glial cell precursors (see Stock No. 003771), this mutant mouse strain may be useful in studies of neurogenesis. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published r ..... | ||
| 004860 | B6.129-Ogttm1Gwh/J | Cryopreserved - Ready for recovery |
| These mice possess loxP restriction sites on either side of the exon encoding amino acids 206-232 of the X-linked Ogt gene. Female mice bearing two copies of the floxed allele, and males carrying one, are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. At least one intact, functional Ogt allele is required for embryonic stem cell viability and subsequent ontogenic events, as well as the viability and function of germ cells, neurons, thymoyctes, fibroblasts. When used in conjunction with a Cre recombinase-expressing strain, this mutant is useful in generating tissue-specific mutants of the floxed gene.
For example, when crossed to a strain expressing Cre recombinase in thymocytes (see Stock No. 003802), this mutant mouse strain may be useful in studies of intracellular glycosylation and T cell apoptosis. When crossed to a strain expressing Cre rec ..... | ||
| 005697 | B6.129-Otx1tm4(cre)Asim/J | Cryopreserved - Ready for recovery |
| This strain expresses Cre recombinase from the targeted locus. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes have a perinatal lethal phenotype. Expression of the Cre recombinase gene (mRNA) under the control of the endogenous gene promoter, is detected in the lateral midbrain of embryonic day 10.5 aged embryos and in the presumptive alar-basal plate boundary of embryonic day 12.5 aged embryos, closely mimicking the endogenous gene expression pattern. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination is first detected at embryonic day 8.7. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study developmentally critical gene function in the brain. | ||
| 008397 | B6.129-Pcyt1atm1Irt/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exons 4 and 5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 4 and 5 deleted in the cre-expressing tissue(s). When bred to a strain with inducible Cre recombinase expression in the myeloid cell lineage (see Stock No. 004781 for example), this mutant mouse strain may be useful in studies of atherosclerosis. | ||
| 005702 | B6.129-Pik3c2btm1Pkha/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exons 3-5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain can be used to generate tissue-specific mutants of the floxed allele. | ||
| 005357 | B6.129-Sema3ftm1.1Ddg/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites 4kb upstream of exon 1 and 1 kb downstream of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to a strain expressing Cre recombinase in neuronal cells (see Stock No. 003966 for example), this mutant mouse strain may be useful in neurobiology research. | ||
| 008133 | B6.129-Sncbtm1Sud/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, normal in size and do not display any gross physical or behavioral abnormalities, but breed very poorly. Protein levels are normal. When bred to a strain expressing Cre recombinase, this mutant mouse strain may be useful in studies of presynaptic proteins and synaptic vesicles. | ||
| 006897 | B6.129-St3gal1tm2Jxm/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this floxed targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele. | ||
| 006898 | B6.129-St3gal2tm2Jxm/J | Cryopreserved - Ready for recovery |
| Mice homozygous for the floxed targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele. | ||
| 006901 | B6.129-St6gal1tm2Jxm/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this floxed targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele. | ||
| 006490 | B6.129S4-Abcb7tm1Mdf/J | Cryopreserved - Ready for recovery |
| Homozygous mice are viable and fertile with no reported neurological or hematological abnormalities. These mutant mice have loxP sites flanking exons 9 and 10 of the endogenous gene. When bred to Cre recombinase expressing mice, exons 9 and 10 are deleted in the offspring dependent on the tissue specificity of the Cre recombinase expressing parent. The donating investigator reports that the null allele is not transmissible due to an effect on the extraembryonic tissues. This mutant may be useful in studying cytosolic Fe-S cluster assembly and metabolism, Friedreich ataxia, anemia, and hematopoiesis.
When bred to a strain expressing Cre recombinase in liver (see Stock No. 003574 for example), this mutant mouse strain may be useful in studies of hepatocyte iron metabolism.
When bred to a strain expressing Cre recombinase in epiblast derived cells (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 007671 | B6.129S4-Fgfr1tm5.1Sor/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 4 of the targeted gene. Exon 4 is the first exon found in all reported Fgfr1 splice variants. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 and the neomycin selection cassette deleted in the cre-expressing tissue(s). This mutant mouse strain may be useful in studies of fibroblast growth factor (Fgf) cellular signaling during embryonic development. | ||
| 003541 | B6.129S4-Ntf3tm2Jae/J | Cryopreserved - Ready for recovery |
| This strain has loxP sites located on either side of the Ntf3 exon 2. Mice homozygous for this allele are viable, fertile and without behavioral abnormalities. If this strain is crossed with a transgenic strain bearing Cre recombinase, expression of the Ntf3 gene in offspring can be blocked in a tissue-specific manner depending on the promoter controlling the recombinase. Strains under the control of the rat nestin promoter block expression in the central nervous system; see strains 002275 and 002276.
When bred to a strain expressing Cre recombinase in skeletal muscle (see Stock No. 007893 for example), this mutant mouse strain may be useful in studies of muscle spindle development | ||
| 005897 | B6.129S4-Ppardtm1Rev/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 4 of the targeted gene is deleted in the tissue of interest, leading to premature termination of the translation product upstream of the DNA binding domain. This strain may be useful in generating tissue-specific mutants of the floxed allele for use in studies including embryo development, adipocyte physiology, fat metabolism and storage, inflammation, and cancer. | ||
| 007583 | B6.129S4-Slc17a6tm1Rpa/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 2 in the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. They show ~30% less expression of the targeted gene than wildtype mice, however. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in eliminating expression in a tissue-specific manner (widespread deletion of expression is lethal). | ||
| 008572 | B6.129S6(Cg)-Kdm5atm1Kael/J | Cryopreserved - Ready for recovery |
| 006042 | B6.129S7-Efnb2tm2And/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 1 of the targeted gene is deleted in the tissue of interest. These mutant mice may be useful in studying the cellular and molecular mechanisms underlying vasculogenesis and angiogenesis, the topography of neovascularization, and adult neovascularization, including tumor angiogenesis. To test the effectiveness of this model, these mutant mice were bred to an endothelial-specific Cre-expressing transgenic mice, Tg(Tek-cre)12Flv (Stock No. 004128) . Offspring homozygous for the Cre-mediated exon 1 deletion show angiogenic remodeling defects and embryonic death identical to homozygous Efnb2tm1And mice (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 004665 | B6.129X1(FVB)-Hnf4atm1.1Gonz/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Similarly oriented loxP sites are positioned in introns 3 and 5 of the targeted gene. When used in conjunction with a Cre recombinase-expressing strain, this mouse can be used to generate tissue-specific mutants. Cre-directed recombination results in the deletion of the loxP-flanked sequence causing a splicing event between exons 3 and exon 6. A frame shift occurs generating a premature stop codon. A putative truncated protein may be translated, but such a protein would lack the A and T box motifs necessary for high-affinity binding to DNA. This mouse mutant may be useful in studies related to lipid homeostasis.
When bred to a strain expressing Cre recombinase in pancreatic beta cells (see Stock No. 003573 for example), this mutant mouse strain may be useful in studies of insulin ..... | ||
| 006922 | B6.Cg-Sfpi1tm2Dgt/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for this "PU.1F" conditional allele are viable and fertile. When PU.1F mice are bred to mice expressing Cre recombinase, exons 4-5 of the targeted gene are deleted in the cre-expressing tissues in the offspring. These mice may be useful in studying T cell lymphoma, AML and other cancers, as well as transcription factors, hematopoiesis, and the development of multiple cell lineages. For example, when PU.1F mice are crossed with mice expressing the interferon- or dsRNA-inducible Mx1-cre transgenic mice (see Stock No. 003556), this mutant mouse strain may be useful in studies of hematopoietic stem cells. NOTE: Despite these mice being backcrossed onto the C57BL/6 genetic background, occasional albino pups may be observed. The donating investigator confirms this observation and suggests the targeted mutation may have an as of yet uncharacterized effect upon coat color ..... | ||
| 006663 | B6.Cg-Tg(Eno2-cre)39Jme/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this NSE39-Cre transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These NSE39-Cre mice harbor a transgenic insert consisting of the cre recombinase gene under the control of the promoter region of the rat neuron specific enolase (NSE or Eno2) gene. As such, Cre recombinase activity is directed to neurons with expression in many tissue types. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome.
Specifically, these NSE39-Cre transgenic mice may also be useful in studies of spinal muscular atrophy (SMA) along with mice harboring a conditional (floxed) Smn1 gene (see Stock No. 006138 or Stock No. 006146).
Additional SMA strains expressing cre in striated muscle are av ..... For more information please see the full phenotype on the strain data sheet | ||
| 006889 | B6.Cg-Tg(Lck-cre)I540Jxm/J | Cryopreserved - Ready for recovery |
| Homozygous mice are viable and have no major defects. This transgenic strain expresses Cre recombinase in thymocytes at lower abundance than line 548-O (Stock No. 003802), resulting in sub-stoichiometric recombination and yielding chimeric cell populations in vivo. In contrast, Lck-Cre transgenic line 548-O recombines loxP-flanked alleles specifically in thymocytes at very high efficiency, up to 99+% as compared with other Lck-Cre transgenic lines. | ||
| 003967 | B6.Cg-Tg(Rbp3-cre)528Jxm/J | Cryopreserved - Ready for recovery |
| These transgenic mice express Cre recombinase under the direction of a Rbp3 promoter. Homozygous mice are prone to eye defects and females may be infertile. Recombinase activity is detected in photoreceptor cells. | ||
| 003966 | B6.Cg-Tg(Syn1-cre)671Jxm/J | Cryopreserved - Ready for recovery |
| These transgenic mice express Cre recombinase under the direction of a synapsin promoter. Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity is detected in neuronal cells by embryonic day 12.5. | ||
| 005630 | B6.Cg-Tg(Thy1-EYFP)15Jrs/J | Cryopreserved - Ready for recovery |
| These transgenic mice conditionally express Enhanced Yellow Fluorescent Protein (EYFP) under the direction of the mouse thymus cell antigen 1, theta, promoter. Expression of the EYFP gene is blocked by a loxP-flanked STOP fragment placed between the promoter and EYFP gene. Cre-mediated excision of the STOP cassette results in expression of EYFP in motor neurons. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. For example, when crossed to a strain with widespread expression of Cre recombinase (see Stock No. 003376), this mutant mouse strain may be useful in studies of synaptic function. | ||
| 008735 | B6.Cg-Tg(Wap-cre)11738Mam/JKnwJ | Cryopreserved - Ready for recovery |
| WAP-Cre transgenic mice are viable and fertile, with expression of P1 Cre recombinase under the control of the mouse Wap (whey acidic protein) promoter. When bred with other mutant mice containing loxP-flanked sequence(s), Cre-mediated recombination will result in deletion of the floxed sequence(s) in mammary gland epithelium. Deletion of floxed sequences is reported in brain tissue from WAP-Cre mice on a mixed genetic background but has not been examined in this strain. Maximum Cre recombinase activity is achieved during pregnancy and lactation, but in virgin mice small numbers of mammary epithelial cells show evidence of estrus cycle-related Cre recombinase activity. These WAP-Cre transgenic mice may be useful in generating conditional mutations in mammary glands for studying mammary physiology and processes such as breast development and breast cancer.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently ..... | ||
| 002982 | B6.Cg-Tg(xstpx-lacZ)32And/J | Cryopreserved - Ready for recovery |
| This mutant, when crossed with a cre transgenic, will express lacZ in cells where cre is expressed to remove the STOP of translation section which lies between the 2 loxP sites. LacZ expression is restricted to neural and skeletal muscle tissue and heart by the chicken beta-actin promoter that is driving the reporter. | ||
| 003394 | B6.FVB-Tg(Zp3-cre)3Mrt/J | Cryopreserved - Ready for recovery |
| This is a transgenic line in which cre expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene. This promoter normally directs expression exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain would be useful for deleting a floxed sequence specifically in the female germ line. Homozygotes females produce small litters (3.7 pups/litter; Genesis 26:110-112, 2000). An alternative transgenic strain that bears a similar transgenic construct and produces larger litters (~6 pups/litter) is available: C57BL/6-Tg(Zp3-cre)93Knw/J (Stock No. 003651). | ||
| 003552 | B6129-Tg(Wap-cre)11738Mam/J | Cryopreserved - Ready for recovery |
| This transgenic strain expresses P1 Cre recombinase under the control of the Wap (whey acidic protein) promoter. In mammary gland tissues, the Wap promoter directs expression to secretory epithelium. A maximum of Cre mediated recombination is achieved during pregnancy and lactation, but recombined cells are still present after involution and complete remodeling of the gland. Cre recombinase expression is not entirely restricted to mammary gland, however. A limited amount of Cre activity has been reported in brain tissue. | ||
| 006394 | B6;129-Apba2tm1Sud Apba3tm1Sud Apba1tm1Sud/J | Cryopreserved - Ready for recovery |
| Triple homozygous knock-in mice are viable and fertile and and do not display any gross physical or behavioral abnormalities. Expression of all three proteins is normal. When crossed with a cre deleter strain that eradicates protein expression, Apba1/Abcba2 (Apba1/2) double knockout and Apba1/2/3 triple knockout mice exhibit a high percentage of postnatal lethality (only ~20% of the mice survive). Apba1/2 mice are visually indistinguishable from their littermates and display no major alterations in breathing, movements or reaction to stimuli several hours after birth, but fail to nurse and die within 24 hours. Their brains are morphologically and structurally normal. Quantitation of 18 neuronal proteins fails to reveal significant changes. Surviving mice show reduced weight gain and exhibit movement dysfunction. Cultured neurons from triple knock-in neonates show impairments in presynaptic neurotransmitter release after treatment with lentiviral cre. Apba1/3 ..... For more information please see the full phenotype on the strain data sheet | ||
| 006382 | B6;129-Casktm1Sud/J | Cryopreserved - Ready for recovery |
| Homozygous floxed mice are viable and fertile, but females do not thrive. The body size of mutants is significantly smaller than littermate controls and they exhibit a slightly increased mortality. Knock-in mice are hypomorphs and protein is expressed at less than 30% of normal levels. Crossing of the floxed mutants with mice expressing cre recombinase in the male germline excises the floxed exon and a neomycin resistance gene cassette to create a complete knockout of the gene. Knockout homozygotes die within a few hours of birth. They exhibit a partially penetrant cleft palate syndrome and increased apoptosis in the thalamus, but display no other major developmental changes or deficits in basic electrical properties of their neurons.
When bred to a strain expressing Cre recombinase in the male germline (see Stock No. 003328 or 007252 for example), this mutant mouse str ..... | ||
| 004604 | B6;129-Ctnna1tm1Efu/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
When bred to a strain expressing Cre recombinase in the female germline (see Stock No. 003551 for example), this mutant mouse strain may be useful in studies of mammary epithelium. When bred to a strain expressing Cre recombinase in the mammary gland (see Stock No. 003552 for example), this mutant mouse strain may be useful in studies of mammary epithelium. When bred to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771 for example), this mutant mouse strain may be useful in studies of the cerebral cortex and the hedgehog signalling pathway. | ||
| 008452 | B6;129-Eif2ak4tm1.1Dron/J | Cryopreserved - Ready for recovery |
| Mice homozygous for the GCN2.KO4conditional allele (also called GCN2.KO4c) are viable and fertile, with loxP sites flanking exon XII of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (containing amino acid residues 606-648 encoding the critical lysine 618 required for kinase activity) deleted in the cre-expressing tissue(s). GCN2 is a protein kinase that phosphorylates eIF2 (eukaryotic initiation factor 2) in response to environmental stresses (amino acid starvation, proteasome inhibition and UV irradiation) resulting in a reduction of global translation and activation of stress-related transcription factors (such as NF-kappaB). These GCN2.KO4conditional mice may be useful in studies related to eIF2 phosphorylation in response to environmental stresses. Of note, mice with a traditional "knockout" (deletion of exon XII) are also available (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 008620 | B6;129-Fzd5tm2Nat/J | Cryopreserved - Ready for recovery |
| This is a conditional allele of the frizzled 5 gene in which the coding region was flanked by loxP sites and an alkaline phosphatase (AP) reporter was placed downstream. Cre-mediated recombination eliminates the coding region in a tissue-specific manner, ablates expression of the gene, and causes alkaline phosphatase (AP) to be expressed under the control of the frizzled 5 promoter. If both floxed alleles are excised, mutants are embryonic lethal at day 10 due to a placental defect. If recombination is mediated by an embryonic tissue-specific Cre, such as Sox2-Cre (e.g. Stock No's. 004783, 008454), mice show a loss of cells in the parafasicular nucleus (PFN) of the thalamus and defects in eye development.
When bred to a strain expressing a tamoxifen inducible Cre recombinase (see Stock No. 004847 ..... | ||
| 009076 | B6;129-Gcnt1tm2Jxm/J | Cryopreserved - Ready for recovery |
| 006885 | B6;129-Man2a1tm2Jxm/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this floxed targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to Cre transgenic strains, the loxP-flanked exon is recombined and deleted where Cre is expressed, producing a null allele. | ||
| 006088 | B6;129-Mcl1tm3Sjk/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for the targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator indicates that homozygous males have severely reduced fertility for unknown reasons, while females have normal fertility. Endogenous protein expression is unaffected by the inserted loxP sequences. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 1 of the targeted gene is deleted in the tissue of interest. These mutant mice may be useful in studying global, temporal, or tissue-specific deletion of the endogenous gene, particularly in studies of immune function, including apoptosis, B and T cell development, and bone marrow cell differentiation. When bred to a strain with the targeted null allele (Stock No. 006072) and a strain with a Cd19 null allele and expressing Cre recombinase during th ..... | ||
| 006894 | B6;129-Mgat4atm1Jxm/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this floxed targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exon is deleted by cre expression to produce a null allele. | ||
| 006933 | B6;129-Mycntm1Psk/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 and 3 deleted in the cre-expressing tissue(s).
For example, when crossed to a strain expressing Cre recombinase in neuronal and glial cell precursors (see Stock No. 003771), this mutant mouse strain may be useful in studies of neurogenesis. | ||
| 008476 | B6;129-Ncstntm1Sud/J | Cryopreserved - Ready for recovery |
| Floxed homozygous mice express intact nicastrin gene. They are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s). General deletion in all tissues results in E9.0 embryonic lethality. g-secretase activity is abolished in cells derived from homozygous cre-recombined mice. These mice are useful to study the role of g-secretase in various tissues in combination with tissue specific cre lines. | ||
| 008363 | B6;129-Nefltm1(cre/Esr1)Nat/J | Cryopreserved - Ready for recovery |
| These targeted mutation mice carry a tamoxifen-inducible Cre cassette knocked into the 3' UTR of the gene. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating 4-hydroxytamoxifen-induced, Cre-mediated targeted deletions specifically in large neurons. Although the donating investigator reports that homozygous males may not be fertile and homozygous females may have reduced fertility, homozygous colonies at The Jackson Laboratory have no reported fertility problems. | ||
| 008349 | B6;129-Ptger3tm1Csml/J | Cryopreserved - Ready for recovery |
| 007855 | B6;129-Rims2tm1.2Schc/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this floxed targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exon 5 is deleted by cre expression to produce a "RIM2alpha" isoform null allele. This strain may be helpful in the analysis of the function of alpha-RIMs in specific brain regions. | ||
| 004162 | B6;129-Scaptm1Mbjg/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for this targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. They posses a loxP-flanked neomycin resistance cassette located 3 kb upstream of exon 1. A third loxP site is located in intron 1. When used in conjunction with a Cre recombinase-expressing strain, this strain is appropriate for generating tissue-specific knockout mutants of Scap. This mutant mouse strain represents a model that may be useful in studies related to cholesterol and fatty acid homeostasis. | ||
| 006099 | B6;129-Sfpi1tm1.2Dgt/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for the targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. Endogenous protein expression is unaffected by the loxP sequences flanking an upstream regulatory region (URE). When bred to mice with a cre recombinase gene under the control of a promoter of interest, the URE of the targeted gene is deleted in the tissue of interest. Deletion of this "floxed" URE may be useful in studying T cell lymphoma, AML and other cancers, as well as transcription factors, hematopoiesis, and development of multiple cell lineages. | ||
| 008366 | B6;129-Smad1tm1Abr/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 2 in the targeted gene. Mice that are homozygous for the floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. Homozygous knock out mice are embryonic lethal. Literature associated with this gene suggests the mice may be useful in studies of reproduction, development, the cardiovascular system and cancer. | ||
| 008294 | B6;129-Syt11tm1Sud/J | Cryopreserved - Ready for recovery |
| These transgenic animals carry a FLAG-(C)4-FLAG insertion in exon 2 of the gene. Homozygotes are viable and have no obvious phenotype. Mutant protein expression levels are similar to those of the wildtype protein. Expression is brain-specific and localized in the neuronal soma and dendrites (presumably on cargo vesicles). Protein can be recognized using FLAG antibody by Western blot analysis, but not in histological sections or labeled cultures. No significant N-ethyl-maleimide-sensitive factor attachment protein receptor (SNARE) binding is observed in immunoprecipitation (IP) assays. Exon 2 is flanked by loxP sites. When crossed with a Cre recombinase-expressing strain, tissue-specific expression can be eliminated by deletion of exon 2. This strain may be useful in studies of neurotransmitter release mechanisms. | ||
| 008295 | B6;129-Syt5tm2Sud/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this Syt5 (synaptotagmin V (NM_016908; GI:31560793); also commonly referred to as synaptotagmin IX) targeted knock-in are viable and fertile and do not display any gross physical or behavioral abnormalities. Mice carry GFP "emerald" fused to the first exon of the targeted gene, and exon 2 is flanked by loxP sites. GFP expression is primarily localized in the limbic system and striatum of the brain. When crossed with a Cre recombinase-expressing strain, offspring are produced with tissue-specific deletion of Syt5 and which lack GFP expression, suggesting this creates a null allele. This strain may be used to study Ca2+ sensors involved with fast neurotransmitter release. | ||
| 008532 | B6;129-Thtm1(cre/Esr1)Nat/J | Cryopreserved - Ready for recovery |
| This creER knock-in line can be used to produce target gene recombination specifically in dopaminergic neurons following 4-hydroxytamoxifen treatment. Heterozygotes and homozygotes are normal in size and viability. | ||
| 008678 | B6;129-Ubbtm1Rrk/J | Cryopreserved - Ready for recovery |
| Mice heterozygous for the targeted allele are viable and fertile. This polyubiquitin B (Ubb) mutation is characterized by a GFP-puror fusion protein "knock-in" allele that also abolishes endogenous gene function. Direct visualization of GFP fluorescence is observed in ovaries, testes, hypothalamus (arcuate nucleus) and cerebral cortex. Homozygotes have no Ubb mRNA observed in the various tissues tested, and are viable but sterile due to failure of germ cells to progress through meiotic prophase I and hypogonadism. Homozygotes also exhibit a complex metabolic phenotype initially characterized by dysfunction of neurons within the central nervous system accompanied by retarded perinatal growth that progresses to adult-onset obesity linked to selective hypothalamic neurodegeneration. Homozygotes also develop adult-onset hyperleptinemia (but normal levels of circulating glucose and insulin) as a consequence of increased fat content. These Ubb-mutant mice may be useful in studyin ..... For more information please see the full phenotype on the strain data sheet | ||
| 008531 | B6;129-Vamp2tm1(cre/Esr1)Nat/J | Cryopreserved - Ready for recovery |
| This creER knock-in line can be used to produce target gene recombination in many diverse types of neurons following 4-hydroxytamoxifen treatment. Heterozygotes are normal in size and viability. Homozygotes of both genders have reduced viability. | ||
| 006668 | B6;129P2-Omptm4(cre)Mom/MomJ | Cryopreserved - Ready for recovery |
| The coding region and part of the 3' non-translated region of the Omp gene was replaced by Cre. The targeted mutation results in a knockout. Mature olfactory sensory neurons express the Cre recombinase at high levels. Homozygous mice are subfertile. As an example, when crossed to a strain with widespread expression of GFP and a loxP-flanked Bgeo reporter (see Stock No. 004178), this mutant mouse strain may be useful in lineage tracing. | ||
| 006568 | B6;129P2-Terf2tm1Tdl/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exons 1 and 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele that can result in acute telomere dysfunction.
For example, when crossed to a strain expressing interferon inducible Cre recombinase (see Stock No. 003556), this mutant mouse strain may be useful in studies of telomere damage response. | ||
| 003902 | B6;129S-Mttptm2Sgy/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites located 2.5 kb 5' of exon 1 and flanking a neomycin resistance gene inserted into intron 1 of the Mttp gene. Mice that are homozygous for this floxed Mttp allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to a strain expressing interferon inducible Cre recombinase in liver tissue (see Stock No. 003556 for example), this mutant mouse strain may be useful in lipoprotein research. | ||
| 004596 | B6;129S1-Smarcb1tm3Sho/J | Cryopreserved - Ready for recovery |
| This strain contains loxP sites, in opposing orientation, flanking the targeted gene resulting in an reversible Cre-mediated conditional null allele. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with an inducible Cre recombinase-expressing strain, this strain is useful in generating mutants of the floxed allele that exhibit partial penetrance. In the presence of high levels of Cre protein, the orientation of the floxed allele is continually in a reversible reaction. All progeny resulting from a cross of these mice with Mx-Cre mice developed T-cell lymphoma or rhabdoid tumors at a median onset of age 11 weeks. The rhaboid tumors exhibited in these progeny mice closely resemble human malignant rhaboid tumors. | ||
| 003309 | B6;129S4-Gt(ROSA)26Sortm1Sor/J | Cryopreserved - Ready for recovery |
| Mice heterozygous or homozygous for the Gtrosa26tm1Sor targeted mutation may be used to test the tissue/cellular expression pattern of the cre transgene in any transgenic strain carrying cre under the regulation of a specific promoter. Cre expression results in the removal of a loxP-flanked DNA segment that prevents expression of a lacZ gene. When crossed with a cre transgenic strain, lacZ is expressed in cells/tissues where cre is expressed. | ||
| 008303 | B6;129S4-Tcf3tm4Zhu/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of the exons of the targeted gene that encode the bHLH region of the E12 and E47 isoforms. Transcription stop sequence and sequence encoding IRES-beta-galactosidase was inserted downstream of the loxP site flanked sequence. This strain can serve as a reporter strain, with successful Cre excision being indicated by beta-galactosidase expression in Cre-expressing tissues. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the exons encoding the bHLH region of the E12 and E47 isoforms deleted and beta-galactosidase expressed in the cre-expressing tissue(s). | ||
| 008293 | B6;129S6-Pclotm2Sud/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 14 and an Frt-flanked neomycin cassette knocked into intron 14 of the piccolo (presynaptic cytomatrix protein) gene. Homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing mouse, this strain is useful in creating a knockout line (see Stock No. 006391). No overt phenotype has been observed in this strain. | ||
| 003466 | B6;D2-Tg(Sycp1-cre)4Min/J | Cryopreserved - Ready for recovery |
| 003734 | B6;FVB-Tg(GZMB-cre)1Jcb/J | Cryopreserved - Ready for recovery |
| This strain is one component of a Cre-recombinase mediated mouse model system that allows irreversible labeling of antigen-stimulated T cells with surface expressed ALPP (placental alkaline phosphatase). Cre recombinase is expressed under control of the human GZMB (granzyme B) promoter which directs specific expression in activated T cells. When this strain is bred with a Cre-recombinase expressing strain the loxP-flanked STOP fragment is excised. This strain represents an effective tool for generating tissue specific-targeted mutants. | ||
| 004426 | B6;SJL-Tg(Cga-cre)3Sac/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the Cre recombinase under the control of the mouse glycoprotein hormone alpha-subunit promoter. Cre recombinase expression is detected in the anterior and intermediate lobes of the pituitary gland, as well as in cardiac and skeletal muscle. Low to no level of expression is detected in the posterior pituitary, lungs, kidneys, brain, adrenal glands and gonads. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the anterior pituitary. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study developmentally critical gene function in the anterior pituitary. | ||
| 003554 | B6;SJL-Tg(Col2a1-cre)1Bhr/J | Cryopreserved - Ready for recovery |
| This strain expresses Cre recombinase in a chondrocyte-specific pattern under the control of a Col2a1 promoter. | ||
| 007041 | B6Ei.129P2-Nr5a1tm2Klp/EiJ | Cryopreserved - Ready for recovery |
| Mice homozygous for this floxed allele are viable and fertile. These mutant mice have loxP sites flanking the C-terminal coding exon. When bred to Cre-recombinase expressing mice, offspring will have a deletion of this exon in the cre expressing tissue(s). These floxed mice may be useful in studying steroidogenic factors and pituitary gonadotrope function.
For example, when crossed to a strain expressing Cre recombinase in the anterior and intermediate lobes of the pituitary gland (see Stock No. 004426), this mutant mouse strain may be useful in studies of pituitary gonadotrope function. | ||
| 007252 | B6Ei.129S4-Tg(Prm-cre)58Og/EiJ | Cryopreserved - Ready for recovery |
| 007686 | BKa.Cg-Sox17tm2Sjm Ptprcb Thy1a/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of the exon 3-5 region of the targeted gene. Mice homozygous for this allele are viable and fertile and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. When the floxed allele is excised by crosses with a Tie2 (endothelial-specific receptor tyrosine kinase)-Cre mouse (Stock No. 004128) and the Sox17 targeted mutant EGFP reporter strain (Stock No. 007687), mutants are embryonic lethal around E13.5. Mutant embryos have severe hematopoietic failure and lack definitive hematopoietic stem cells.
When the floxed allele is excised by Mx1 (myxovirus (influenza virus) resistance 1)-Cre (Stock No. 003556) and the Sox17 ..... | ||
| 005673 | C.Cg-Tg(Mx1-cre)1Cgn/J | Cryopreserved - Ready for recovery |
| The Cre recombinase is under the control of the Mx1 promoter. This promoter is silent in healthy mice, but can be induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA (such as poly I:C). When combined with a mutant carrying a gene that has been flanked by loxP recognition sites, the expression of Cre recombinase causes the flanked gene to be removed. This provides researchers with the capability to induce the "knockout" at any time during development. There is ~1% background recombination seen in mice not treated with interferon. The percent deletion of the targeted gene varies depending on tissue type, presumably due to the amount of interferon-responsive cells present or to the availability of interferon in each organ.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an a ..... | ||
| 006244 | C.Cg-Tg(tetO-cre)1Jaw/J | Cryopreserved - Ready for recovery |
| Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of a tetracycline-responsive promoter element (TRE; tetO). When hemizygotes are bred with another transgenic mouse expressing either reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under the control of tissue-specific promoters, Cre recombinase expression and Cre-mediated recombination in the appropriate tissues of the bitransgenic offspring can be regulated with the tetracycline analog, doxycycline. This strain represents an effective tool for generating inducible tissue specific-targeted mutants to study cell lineage during development.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele wa ..... | ||
| 006575 | C57BL/6-Camk2atm1Vyb/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this "falpha-CaMKII" allele are viable and fertile with no reported neurological abnormalities. These mutant mice have loxP sites flanking exon 2 of the endogenous gene. When bred to Cre-recombinase expressing mice, exon 2 is deleted in the resulting offspring dependent on the tissue specificity of the promoter of the cre transgene. For example, when crossed with transgenic mice expressing Cre-recombinase in hippocampal CA3 pyramidal cells (see Stock No. 006474), the resulting offspring show altered neurotransmitter release. These "floxed" mice may be useful for neurological studies such as calcium/calmodulin-dependent protein kinase activity. | ||
| 007895 | C57BL/6-Fastm1Cgn/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this "Fasfl" conditional allele are viable and fertile, with loxP sites flanking exon 9 of the targeted gene. When bred to mice that express Cre recombinase, exon 9 (which encodes the death domain) is deleted in the cre-expressing tissues in the resulting offspring.
These Fasfl mice may be useful in generating conditional mutations for studying many aspects of immune function. For example, when Fasfl mice are crossed to a strain expressing Cre recombinase in B lineage cells (see Stock No. 004126 or 006785 ), this mutant mouse strain may be useful in studies of lymphoproliferative disorder. Similarly, when Fasfl mice are crossed to an interferon inducible strain with widespread Cre recombinase expression (see Stock No. 003556, ..... | ||
| 008304 | C57BL/6-Nrastm1Tyj/J | Cryopreserved - Ready for recovery |
| This targeted mutant strain carries a loxP-flanked stop element in exon 1 and a G12D activating mutation in exon 2 of the neuroblastoma ras oncogene (Nras). This mutation functions as a null allele and has no apparent phenotype. Homozygotes are viable. Cre mediated excision of the floxed stop element causes a significant increase in GTP-bound N-Ras production. This conditional mutant strain may be helpful in further studies of this oncogene. | ||
| 006581 | C57BL/6-Ppp3r1tm1Stl/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this "fCNB1" mutant allele are viable and fertile with no reported neurological abnormalities. These mutant mice have loxP sites flanking exons 2-4 of the endogenous gene. When bred to transgenic mice expressing Cre recombinase, exons 2-4 are deleted in the resulting offspring in only those tissues expressing cre. For example, when crossed with transgenic mice expressing Cre recombinase specifically in the forebrain (similar to Stock No. 005359), the resulting offspring show abnormalities that are strikingly similar to those described for schizophrenia. These "floxed" mice may be useful in studies of calcineurin function in T cells (via NFAT transcription of cytokine genes) and in the central nervous system in, for example, neurite extension, synaptic plasticity, learning and memory, and schizophrenia pathogenesis. | ||
| 006662 | C57BL/6-Tg(ACTB-MAP2K1*K97M)1Stl/J | Cryopreserved - Ready for recovery |
| Hemizygous mice are viable and fertile. These "dnMEK1" mice express a dominant-negative mutant (K97M) form of human MEK1 (synonym: MAP2K1) following Cre-mediated removal of the upstream "Lox-STOP-Lox" cassette; when transgenic mice are bred to a cre-expressing strain, the "floxed stop" cassete is excised in the resulting offspring, and mutant MEK1 expression is observed in the cre-expressing tissue(s). In the absence of Cre recombinase, transgene expression is not detectable in the brains of these "floxed" mice Because the MEK1 mutation abolishes the protein's kinase activity but preserves its ability to interact with ERK1 and ERK2, these transgenic mice may be useful in studying MEK-dependent activation and regulation of ERK, the ERK-MAPK signaling pathway, and neurological studies involving synaptic plasticity and memory. When bred to a strain expressing Cre recombinase in the CA1 pyramidal cell layer of the hippocampus (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 006888 | C57BL/6-Tg(Zp3-cre)1Gwh/J | Cryopreserved - Ready for recovery |
| This is a transgenic line in which Cre recombinase expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene, which is normally expressed exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain is useful for deleting a loxP-flanked genomic segment during oogenesis and thereby producing embryos and offspring that bear the recombined and deleted genomic element(s). | ||
| 007042 | D2.129P2(B6)-Nr5a1tm2Klp/EiJ | Cryopreserved - Ready for recovery |
| Mice homozygous for this floxed allele are viable and fertile. These mutant mice have loxP sites flanking the C-terminal coding exon. When bred to Cre-recombinase expressing mice, offspring will have a deletion of this exon in the cre expressing tissue(s). These floxed mice may be useful in studying steroidogenic factors and pituitary gonadotrope function.
For example, when crossed to a strain expressing Cre recombinase in the anterior and intermediate lobes of the pituitary gland (see Stock No. 004426), this mutant mouse strain may be useful in studies of pituitary gonadotrope function. | ||
| 002981 | DBA/2-Tg(xstpx-lacZ)36And/J | Cryopreserved - Ready for recovery |
| This test strain is used determine the tissue expression pattern of cre transgenic mice. The transgene is loxP-STOP-of-translation-loxP-lacZ driven by the beta-actin promoter. The lacZ is expressed only in cells where the STOP element has been removed by Cre recombinase. LacZ expression is restricted to neural and skeletal muscle tissue and heart by the chicken beta-actin promoter that is driving the reporter. | ||
| 006138 | FVB.129(B6)-Smn1tm1Jme/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this SMNF7 floxed allele are viable and fertile and do not display any gross physical or behavioral abnormalities. Mutant mice exhibit no transcript splicing defects. Cre-mediated recombination of the loxP-flanked sequences results in deletion of exon 7 of the targeted gene. As mutations of this exon are implicated in 95% of all human spinal muscular atrophy (SMA), these mice may be useful in studying SMA or other neuromuscular degenerative diseases.
When crossed to a strain expressing Cre recombinase in neurons (ssee Stock No. 005938, Stock No. 006297, and Stock No. 006663), this mutant mouse strain may be useful as a model of SMA. When crossed to a strain expressing Cre recombinase in striated muscle fibers (see Stock No. For more information please see the full phenotype on the strain data sheet | ||
| 005710 | FVB.129S-Mmp13tm1Werb/J | Cryopreserved - Ready for recovery |
| Mice that are homozygous for this loxP-flanked ("floxed") allele are viable and fertile with normal endogenous gene function. Cre-mediated recombination results in replacement of exons 3-5 of targeted gene with a single loxP site. When bred to cre-expressing transgenic strains, these floxed mutant mice may be used to generate whole mouse or tissue-specific targeted mutants that may be useful in examining extracellular matrix remodeling and bone development. Of note: when these floxed mice are bred to mice containing a beta-actin promoter-driven cre transgene the resulting cre-positive, homozygous-null mice show robust accumulation of cartilage matrix caused by a transient expansion of the hypertrophic zone and increased trabecular bone mass that persists for months. | ||
| 006139 | FVB.Cg-Tg(ACTA1-cre)79Jme/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this HSA-Cre79 transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These HSA-Cre79 transgenic mice have the cre recombinase gene driven by the human alpha-skeletal actin (HSA or ACTA1) promoter. Cre activity is restricted to adult striated muscle fibers and embryonic striated muscle cells of the somites and heart. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in striated muscle-specific deletion of the flanked genome. Specifically, these HSA-Cre79 (or ACTA1-Cre) transgenic mice were originally used to breed with mice heterozygous for a deletion of exon 7 and a loxP-flanked exon 7 mutation on homologous chromosomes of the Smn1 gene (see Stock No. 006138 or Stock No. 006146). The resulting offspring ( ..... For more information please see the full phenotype on the strain data sheet | ||
| 006297 | FVB.Cg-Tg(Eno2-cre)39Jme/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this NSE39-Cre transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These NSE39-Cre mice harbor a transgenic insert consisting of the Cre recombinase gene under the control of the promoter region of the rat neuron specific enolase (NSE or Eno2) gene. As such, Cre recombinase activity is directed to neurons with expression in many tissue types. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome. Specifically, these NSE39-Cre transgenic mice may also be useful in studies of spinal muscular atrophy (SMA) along with mice harboring a conditional (floxed) Smn1 gene (see Stock No. 006138 or Stock No. 006146).
Additional SMA strains expressing cre in striated muscle are available ..... | ||
| 003376 | FVB/N-Tg(ACTB-cre)2Mrt/J | Cryopreserved - Ready for recovery |
| This Cre recombinase strain is under the human beta actin gene promoter. This strain expresses Cre recombinase in all cells of the embryo by the blastocyst stage of development. It is useful as a "deletor" mouse for virtually any genomic sequence flanked by loxP sites. | ||
| 003377 | FVB/N-Tg(Zp3-cre)3Mrt/J | Cryopreserved - Ready for recovery |
| The strain originated on an FVB/N background and is currently on the same background. The investigator maintains the strain by breeding hemizygotes. Homozygotes are subfertile. This is a transgenic line in which cre expression is controlled by the regulatory sequences from the mouse zona pellucida 3 (Zp3) gene, which is normally expressed exclusively in the growing oocyte prior to the completion of the first meiotic division. This strain would be useful for deleting a floxed sequence specifically in the female germ line. | ||
| 005732 | NOD.Cg-Tg(Lck-cre)548Jxm/AchJ | Cryopreserved - Ready for recovery |
| This strain expresses Cre recombinase in thymocytes and is active in T-cells during development. | ||
| 004986 | NOD/ShiLt-Tg(Ins2-cre)3Lt/Lt | Cryopreserved - Ready for recovery |
| This transgenic strain uses the rat insulin 2 promoter to drive expression of Cre recombinase in pancreatic beta cells. Hemizygous mice develop a diabetes that is characteristic of the NOD/ShiLt inbred strain. The investigator reports that insulin expression appears to be moderately suppressed. | ||
| 003855 | NOD/ShiLt-Tg(Ins2-cre)5Lt/LtJ | Cryopreserved - Ready for recovery |
| This strain expresses Cre recombinase under the control of the rat insulin II promoter. Hemizygous mice carrying this transgene are phenotypically normal and overexpresss cre specifically in pancreatic beta cells. Homozygous transgenic mice experience delayed onset diabetes compared to wildtype controls. This transgenic strain is used in combination with mice carrying floxed targeted mutations to create various pancreatic beta cell-specific gene knockouts using the Cre/loxP system. | ||
| 004987 | NOD/ShiLt-Tg(Ins2-cre)6Lt/Lt | Cryopreserved - Ready for recovery |
| 005134 | STOCK Disp1tm2Amc/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele, which is hypomorphic. | ||
| 007924 | STOCK En2tm4(cre/ESR1)Alj/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this En2-CreERT2 mutation are viable but may not breed well (reported as "semi-fertile"). As the Cre-ERT2 fusion gene is inserted into the 5' UTR of the engrailed 2 (En2) locus, tamoxifen-inducible cre activity is controlled by the endogenous En2 regulatory elements. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to OHT. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these En2-CreERT2 mice are bred with mice containing a loxP-flanked se ..... For more information please see the full phenotype on the strain data sheet | ||
| 007674 | STOCK Esrrbtm1.1Nat/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this Nr3b2CKO allele possess loxP sites flanking exon 2 of the targeted gene and are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the exon containing the initiator methionine codon and encoding the N-terminal 132 amino acids (including part of the DNA-binding domain) deleted in the cre-expressing tissue(s). Of note, if the conditional Nr3b2CKO is deleted by Cre recombinase in the placenta and embryo, embryonic lethality will result (placental defect). If the conditional Nr3b2CKO is deleted by Cre recombinase only in the embryo, the resulting mice exhibit an inner ear defect (decreased endolymph production) resulting in deafness and defective balance. These Nr3b2CKO mutant mice may be useful in generating conditional mutations to study disorders of hearing and balance, inner ear development (such as endolymph-producing epithelia withi ..... For more information please see the full phenotype on the strain data sheet | ||
| 005994 | STOCK Mbtps1tm1Jdh/J | Cryopreserved - Ready for recovery |
| These mice carry a targeted mutation in which exon 2 of the targeted gene is flanked by loxP sites. A loxP-flanked ("floxed") neomycin resistance cassette also is inserted downstream in intron 2. Homozygotes are viable and fertile, and the floxed gene appears to function normally. When homozygotes are crossed with transgenic strains expressing Cre-recombinase, cre-mediated recombination of the loxP-flanked sequences can result in one of three genotypes: a) deletion of the neo cassette only, leaving a loxP-flanked second exon and unimpaired endogenous gene function. b) Deletion of exon 2 only, leaving a loxP-flanked neo cassette and no endogenous gene function. c) Deletion of both the neo cassette and exon 2, leaving a single loxP site and no endogenous gene function. When these floxed mutant mice are bred to mice carrying the Mx1-cre transgene (for example, Stock No. 003556), liver- ..... For more information please see the full phenotype on the strain data sheet | ||
| 004192 | STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J | Cryopreserved - Ready for recovery |
| These mice are homozygous for four different induced mutations. The cumulative result of these mutations is a mouse model in which hypercholesterolemia can be reversed. By themselves, the combined presence of the Ldlrtm1Sgy and Apobtm1Sgy targeted alleles results in mice with a high susceptibility to atherosclerosis and total plasma cholesterol levels of approximately 300 mg/dl. A functional microsomal triglyceride transfer protein gene (Mttp) is essential for establishing a hypercholesterolemic condition. By flanking the Mttp gene with loxP sites and including a Mx1-Cre transgene, it is possible to reduce total plasma cholesterol levels from 300 mg/dl to 30 mg/dl upon induction of the Cre recombinase by administering interferon alpha, interferon beta, or synthetic double-stranded RNA. This unique model is useful in research related to the mechanisms and events of atherosclerotic reversal. Mice homozygous for the targeted ..... For more information please see the full phenotype on the strain data sheet | ||
| 006677 | STOCK Olfr151tm28Mom/MomJ | Cryopreserved - Ready for recovery |
| Olfactory sensory neurons that express the olfactory receptor Olfr151 also co-express the Cre recombinase by virtue of IRES-mediated co-translation. | ||
| 005737 | STOCK Ppiftm1Mmos/J | Cryopreserved - Ready for recovery |
| In this strain loxP sites flank exons 3-5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cre-mediated recombination results in the deletion of the loxP-flanked region. This strain represents an effective tool for generating tissue-specific targeted mutants useful in studies examining the consequences of disrupting Ppif-dependent pathways. | ||
| 005550 | STOCK Rac1tm1Djk/J | Cryopreserved - Ready for recovery |
| These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. When bred to a strain expressing Cre recombinase in the myeloid cell lineage (see Stock No. 004781 for example), this mutant mouse strain may be useful in studies of neutrophil function. When bred to a strain expressing a tamoxifen inducible Cre recombinase specific to keratinocytes(see Stock No. 005107 for example), this mutant mouse strain may be useful in studies of stem cell renewal in the epidermis. | ||
| 007570 | STOCK Sim1tm1.2Az/J | Cryopreserved - Ready for recovery |
| Mice homozygous for this "Sim1-floxed exon 1" (2-loxP) conditional allele are viable and fertile, with loxP sites flanking the translation start site and the first 17 amino acids of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for the basic domain of SIM1) deleted in the cre-expressing tissue(s). These "Sim1-floxed exon 1" (2-loxP) mice may be useful in generating conditional mutations for studying basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS) transcription factors, central nervous system development, early-onset/hyperphagic obesity, and regulation of appetite and energy balance. | ||
| 006876 | STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for this Cre-conditional TEL-AML1 (or iZ/EG-TEL-AML1) transgene are viable and fertile. Homozygotes die in utero, presumably due to high lacZ expression. Prior to Cre-mediated excision of the "floxed" STOP sequence, high expression of lacZ is observed in cells and tissues. When bred to Cre recombinase transgenic mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the human TEL-AML1 fusion protein and EGFP in all cre-expressing cells. TEL-AML1 transcripts are not observed in adult organ tissues prior to excision of the floxed sequences. Following Cre-mediated deletion of the STOP sequence (by B6.Cg-Tg(Tek-cre)12Flv/J, Stock No. 004128), Western blot analysis reveals that EGFP levels are well correlated with TEL-AML1 transcript levels. While global expression of TEL-AML1 leads to embryonic lethality (E7.5), hematopoieti ..... For more information please see the full phenotype on the strain data sheet | ||
| 003919 | STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J | Cryopreserved - Ready for recovery |
| These transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being erythrocytes, chondrocytes and adipocytes. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with alkaline phosphatase expression in tissues expressing Cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess cre excision activity at the individual cell level. | ||
| 004453 | STOCK Tg(CAG-cre/Esr1)5Amc/J | Cryopreserved - Ready for recovery |
| These transgenic mice have a tamoxifen-inducible cre-mediated recombination system driven by the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/ESR1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice. Homozygous mice are not viable or fertile. He ..... For more information please see the full phenotype on the strain data sheet | ||
| 005105 | STOCK Tg(Chx10-EGFP/cre,-ALPP)2Clc/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The transgene insert contains a fusion product involving Cre recombinase and an Enhanced Green Fluorescent Protein (EGFP) and a fusion product involving an internal ribosome entry site/human placental alkaline phosphatase under the control of the mouse Chx10, C. elegans ceh-10 homeo domain containing homolog promoter. This strain serves as a multifunctional reporter strain. Expression of the Chx10 BAC, as detected by in situ hybridization, mimics the endogenous Chx10 gene expression pattern. Alkaline phosphatase expression is mosaic, but specific to the retina and Muller glial cells. EGFP expression is detected in the outer neuroblastic layer of the retina at embryonic days 14.5, 17.5 and neonates. When crossed to a cre reporter strain, the resulting mice exhibit mosaicism in reporter gene expression. This mutant mouse str ..... For more information please see the full phenotype on the strain data sheet | ||
| 004692 | STOCK Tg(Hoxb7-cre)13Amc/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are not viable. These transgenic mice express the Cre recombinase under the control of the mouse homeobox B7 enhancer and promoter. Recombination closely patterns the endogenous gene expression. Cre recombinase expression is detected in the mesonephric duct as early as embryonic day 9.5, in the ureteric bud by embryonic day 10.25 and in all ureteric bud epithelial cells by embryonic day 12.5. Low levels of expression are detected in the dorsal root ganglia and the spinal cord. When crossed with a strain containing a loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in the mesonephric duct and its developmental derivatives (the Wolffian duct, the collecting duct epithelium of kidney and ureteral epithelium). This strain represents an effective tool for generating ti ..... For more information please see the full phenotype on the strain data sheet | ||
| 008122 | STOCK Tg(Ins2-cre/Esr1)1Dam/J | Cryopreserved - Ready for recovery |
| These RIP-CreER transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the rat insulin 2, Ins2, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in islet cells of the pancreas. Hemizygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted ..... For more information please see the full phenotype on the strain data sheet | ||
| 008582 | STOCK Tg(Kcnc2-Cre)K128Stl/LetJ | Cryopreserved - Ready for recovery |
| Cre is expressed from the "Kv3.2" promoter in these transgenic mice. When crossed with a lacZ reporter strain, strong expression was seen in the rostral and midline thalamic nuclei (xiphoid, reunions, rhomboid, centromedial, intermediodorsal, paraventricular, paracentral, centrolateral, submedius, mediodorsal, lateral dorsal, anteromedial, suprageniculate, and Paratenial). Moderate expression was observed in other thalamic nuclei: anteroventral, anterodorsal, ventral lateral, ventral anterior, parafascicular, and posterior thalamic group. Weak expression was observed in the lateral posterior,dorsal lateral geniculate, medial geniculate, and ventral posterior thalamic nuclei.
Strong activity was observed also in ventral medial and dorsal medial hypothalamic nuclei, pontine reticular nucleus, mesencephalic fifth, and 12th cranial nerve nuclei. Moderate recombination was also present in the superficial piriform cortex, indusium griseum, dorsal endopiriform nucleus, me ..... | ||
| 003551 | STOCK Tg(MMTV-cre)1Mam/J | Cryopreserved - Ready for recovery |
| This transgenic strain expresses P1 Cre recombinase under the control of the MMTV LTR promoter. The MMTV LTR promoter directs a widespread pattern of expression including the female germline. High levels of recombination have been detected in the virgin and lactating mammary gland, salivary gland, seminal vesicle, skin, and various cells of the immune system. Little background recombination was observed in the lung, kidney, liver and brain tissues. The donating investigator indicates that this strain may be suitable for use in applications where it is desirable to delete genes in the virgin and lactating mammary gland, and other secretory tissues. Furthermore, since this strain expresses cre in the female germline, it can also be utilized to generate a knockout allele from a targeted locus with loxP sites with an efficiency of 100%. | ||
| 002527 | STOCK Tg(Mx1-cre)1Cgn/J | Cryopreserved - Ready for recovery |
| The Cre recombinase is under the control of the Mx1 promoter. This promoter is silent in healthy mice, but can be induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA. When combined with a mutant carrying a gene that has been flanked by loxP recognition sites, the expression of Cre recombinase causes the flanked gene to be removed. This provides researchers with the capability to induce the "knockout" at any time during development. There was ~1% background recombination seen in mice not treated with interferon. The percent deletion of the targeted gene varied depending on tissue type, presumably due to the amount of interferon-responsive cells present or to the availability of interferon in each organ. | ||
| 002858 | STOCK Tg(Nes-cre)1Wme/J | Cryopreserved - Ready for recovery |
| These transgenic mice carry the Cre recombinase gene under the control of the rat nestin promoter. When crossed with a strain carrying a gene flanked by loxP sites, partial deletion of the loxP-flanked allele occurs before embryonic day 10.5 and is detectable in all adult organs examined including germ line cells. Because of the partial deletion, these balancer1 cre transgenic mice, as well as the balancer2 cre transgenic mice (Stock No. 002859), may be used to generate mosaic mice consisting of cells that are either wildtype or mutant for the gene of interest. The proportion of cells that carry the mutant allele varies between tissues and between individual mice. In cases where deletion of a particular gene is lethal, the generation of mosaic mice using these strains can bypass lethality in some mosaic individuals. The balancer2 line induces more variable deletion of loxP-flanked genes than the ..... For more information please see the full phenotype on the strain data sheet | ||
| 002859 | STOCK Tg(Nes-cre)2Wme/J | Cryopreserved - Ready for recovery |
| These transgenic mice carry the Cre recombinase gene under the control of the rat nestin promoter. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing cre, generating a proportion of mosaic animals. All adult organs, including germ-line cells, may be affected. The balancer2 line of transgenic mice show greater variability and instability in their generation of mosaics than the balancer1 line (Stock No. 002858). This variability of the balancer2 line can be of particular benefit if the gene of interest is vital and mosaic individuals are used to bypass lethality. | ||
| 005667 | STOCK Tg(Neurog3-cre)C1Able/J | Cryopreserved - Ready for recovery |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Expression of the transgene is directed by a neurogenin 3 promoter. Tissues where Cre recombinase expression is detected include the small intestine (base of intestinal crypts) and fetal pancreatic epithelial cells. Cre activity has been shown in islets of the adult pancreas, small intestine enteroendocrine cells, endocrine portions of the stomach, all pancreatic endocrine cells, and in some non-endocrine intestinal cells. When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked gene in the tissues that normally express neurogenin 3. | ||
| 006395 | STOCK Tg(Sim1-cre)1Lowl/J | Cryopreserved - Ready for recovery |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Transgene expression is observed in all areas that endogenously express Sim1, including paraventricular hypothalamus and other parts of the brain. When these Sim1-Cre mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequences in Sim1-expressing tissues (including hypothalamus). As such, Sim1-Cre transgenic mice may be useful in studying body weight homeostasis, obesity, leptin metabolism, or as a reporter strain for Sim1-transcription factor activity. Of note, Sim1-Cre mice may also available on a C57BL/6J congenic background (see Stock No. 006451). | ||
| 002471 | STOCK Tg(hCMV-cre)140Sau/J | Cryopreserved - Ready for recovery |
| This strain contains a Cre recombinase gene under the control of a human cytomegalovirus minimal promoter. This promoter directs transcription such that Cre recombinase is expressed in all tissues. | ||
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Please indicate your interest in purchasing any of the strains listed below when they become available for distribution by checking the box next to the strain(s) of interest and then selecting the "Continue" button which leads to an Interest Form.View a Data sheet for New Strains Under Development
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New Strains Under DevelopmentThe Jackson Laboratory serves as a worldwide distributor and national repository for common and rare strains of inbred mice and mice carrying spontaneous mutations or induced mutations (i.e., transgenic, targeted/"knockout", or chemically induced mutations). At any one time, we have over 100 strains at various stages of development and colony expansion. Strains "Under Development" fall into two categories depending on the anticipated demand from the scientific community.
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It is VERY IMPORTANT that you register interest in strains Under Development. The anticipated demand for a strain enables us to determine effectively the distribution plan for each strain Under Development. Registering interest also provides benefits to you (including advance notification of pending availability). Whether a strain is made available from a live colony OR from our cryopreservation repository, you may want to consider the option of Dedicated Supply. To learn more about Dedicated Supply, go to Services.
- Strains that will be made available from a live distribution colony at The Jackson Laboratory.
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