Search Criteria: Research Area is "Research Tools: Cre-lox System: loxP-flanked Sequences"

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JAX® Mice Strains

Stock
Number
Strain Name
 
Strain Description
Standard Supply
003474 B6.129S4-Gt(ROSA)26Sortm1Sor/J
Level 4
Homozygous mice are viable and fertile, with a loxP-flanked DNA STOP sequence preventing expression of the downstream lacZ gene. When crossed with a cre transgenic strain, the STOP sequence is removed and lacZ is expressed in cells/tissues where cre is expressed. These mutant mice may be used as a Cre-reporter strain; to test the tissue/cellular expression pattern of cre transgenic mice.
021444 129-Atmtm2.1Fwa/J
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Phosphoinositide 3-kinaserelated protein kinase (PIKK) family member ATM (Ataxia-telangiectasia mutated) is activated by DNA double-strand breaks and regulates the DNA double-strand break response and repair. These mice possess loxP sites on either side of exons 57-58 of the targeted gene, which encode for the core PIKK kinase domain. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 57-58 deleted in the cre-expressing tissues.
020994 129S-Porcntm1.1Vdv/J
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These Porcn-ex3-7flox mutant mice possess loxP sites flanking exons 3-7 of the X-linked porcupine homolog (Porcn) gene. XPorcn-ex3-7flox/XPorcn-ex3-7flox females and XPorcn-ex3-7flox/Y males are viable and fertile. PORCN encodes an endoplasmic reticulum protein involved in wingless-related MMTV (Wnt) signaling and has an essential role in gastrulation during early embryonic development. Dominant loss-of-function mutations in PORNC have been associated with focal dermal hypoplasia (FDH), also known as Goltz syndrome or Goltz-Gorlin syndrome, an X-linked disorder that predominantly affects females. FDH is characterized by a wide array of abnormalities including defects of skeleton, skin, hair, eyes, ears, and ectodermal appendages. When bred to mice that express Cre recombinase, resulting offspring will have exons 3-7 deleted in the cre-expressing tissues. Widespread deletion results in increased early em .....
For more information please see the full phenotype on the strain data sheet
017790 129S-Slc40a1tm2Nca/J
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Ferroportin is the only known iron exporter and is essential for iron homeostasis and iron absorption in the intestine. Mutations in the human ferroportin gene, SLC40A1 (solute carrier family 40 (iron-regulated transporter), member 1), can cause type 4 hemochromatosis. These mice possess loxP sites on either side of exons 6 and 7 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 6 and 7 deleted in the cre-expressing tissues.

When bred to a strain with inducible Cre recombinase expression in the intestine, this mutant mouse strain may be useful in studies of intestinal iron absorption and anemia.

When bred to a strain expressing Cre recombinase in epiblast derived tissues (Meox2tm1(cre)Sor; see Stock No. > .....
For more information please see the full phenotype on the strain data sheet

012888 129S-Wlstm1.1Lan/J
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These Wls floxed mutant mice possess loxP site before the ATG start site in the 5' untranslated region of exon 1 and another upstream of exon 2 of the wntless homolog (Drosophila) (Wls) gene. Mice that are homozygous for this allele are viable, fertile, and normal in size. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in cre-expressing tissues, abolishing gene function. When bred to a strain expressing Cre recombinase in the germline, homozygotes fail to develop mesoderm and are embryonic lethal by E8.5. When bred to a strain expressing Cre recombinase in pancreatic precursors, the mutant mice develop pancreatic hypoplasia. When bred to a strain expressing Cre recombinase in neural crest cells, the mutant mouse strain has severe brain malformations and exhibit postnatal lethality. This mutant mouse strain is useful to study Wnt signaling in any organ or tissue that can be targeted .....
For more information please see the full phenotype on the strain data sheet
008242 B6(Cg)-Gt(ROSA)26Sortm4(Ikbkb)Rsky/J
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Mice homozygous for the R26StopFLikk2ca conditional allele are viable and fertile, with a loxP-flanked STOP cassette preventing transcription of the downstream bicistronic sequences (a FLAG-tagged, constitutively active form of IKbkb (IKK2ca) and EGFP). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of the IKK2ca as well as EGFP fluorescence. Expression of IKK2ca leads to constitutively active NF-kappaB transcription factor activity. These R26StopFLikk2ca mice allow inducible expression of an activated form of Ikbkb (IKK2 or IKK-beta) and subsequent activation of the NF-kappaB transcription factor pathways.

For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this .....
For more information please see the full phenotype on the strain data sheet

014175 B6(Cg)-Irf8tm1.1Hm/J
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These mice possess loxP sites on either side of exon 2, which encodes the DNA binding domain. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in B lymphocytes (see Stock No. 006785 for example), this mutant mouse strain may be useful in studies of B cell differentiation.

016235 B6(Cg)-Narfltm1.1Fsl/J
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These Iop1flox mutant mice possess loxP sites flanking exon 1 of the nuclear prelamin A recognition factor-like (Narfl or Iop1) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. IOP1 is required for cytosolic iron-sulfur protein assembly pathways. Iron-sulfur proteins are involved in the Krebs Cycle, oxidative phosphorylation, translation, iron regulatory pathways, and purine metabolism. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissues. This strain may be useful for conditional deletion of IOP1 to study the cytoplasmic iron-sulfur complex assembly pathway.
023803 B6(Cg)-Nr6a1tm1c(EUCOMM)Wtsi/J
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Nr6a1 (nuclear receptor subfamily 6, group A, member 1; also called Gcnf) is expressed in fetal ovarian cells, peaking at embryonic day 14.5 (E14.5). Both somatic stem cells and embryonic stem cells require NR6A1 to silence the activities of Pou5f1 (POU domain, class 5, transcription factor 1; also called Oct4) and Nanog (Nanog homeobox) in the transition from pluripotent to differentiated cell state. Interestingly, the gene is not required for this process in fetal ovarian germ cells and it is not required for initiation of meiosis and oogenesis.

Exon 7 of the Nr6a1 gene, encoding the beginning of the ligand binding domain, is flanked by loxP sites in this mutant strain. Homozygous floxed animals are phenotypically normal for gross morphology, behavior, fertility, and viability.

Cre-mediated recombination can be carried out to produce a knockout allele in which exon 7 is deleted. Crosses with tamoxifen-inducible UBC-cre/ERT2 mice (e.g. S .....
For more information please see the full phenotype on the strain data sheet

008771 B6(Cg)-Rorctm3Litt/J
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These mice possess loxP sites on either side of the exon 3-6 region of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of orphan nuclear receptor RORgamma, including the RORgammaT thymus-specific isoform. Germline deletion of RORgamma and RORgammaT induces an abnormality in lymphoid organ development, survival of CD4+ CD8+ double positive thymocytes, and differentiation of Th17 helper T cells.
017890 B6(Cg)-Shank3tm1.2Bux/J
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These mutant mice harbor a deletion of the SH3/ankyrin domain gene 3 ankyrin repeat domains (exons 4-9). This deletion prevents full-length Shank3 expression; Shank3 mRNA/protein is absent in homozygous postsynaptic density (PSD) fractions, and reduced 50% in heterozygous PSD fractions. Heterozygous mice are viable and fertile with no observed gross brain structure defects or seizures. Heterozygous mice exhibit reduced basal neurotransmission. Heterozygous males display less social sniffing and emit fewer ultrasonic vocalizations during interactions with estrus female mice. Following theta-burst pairing (coincident pre- and postsynaptic stimulation), heterozygous mice have impaired long-term potentiation and altered spine remodeling. Homozygous mice are viable with subtle motor abnormalities. The complete phenotype of homozygous mice is not yet characterized to date (April 2012).
023350 B6(Cg)-Stim1tm1Rao/J
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The stromal interaction molecule 1 (Stim1) gene encodes a calcium Ca2+ sensing transmembrane protein located in the endoplasmic reticulum (ER) that becomes activated upon decrease of ER luminal Ca2+. The activated STIM1 protein then translocates into ER plasma membrane junctions where it activates plasma membrane Orai Ca2+ channels. These mice possess loxP sites on either side of the Stim1 exon 2. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6 (??) genetic background.

When bred to a strain with Cre recombinase expression in T cells (see Stock No. 017336 for example), this mutant mouse strain may be useful in studies of .....
For more information please see the full phenotype on the strain data sheet

021773 B6(FVB)-Cxcl12tm1.1Link/J
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Cxcl12 (chemokine (C-X-C motif) ligand 12) regulates many hematopoietic and non-hematopoietic cell types. CXCL12 is constitutively expressed at high levels in many bone marrow stromal cell types, where it contributes to both hematopoietic stem cell (HSC) and lymphoid progenitor maintenance. Exon 2 of the mouse gene is flanked by loxP sites in this conditional targeted mutation. Cre recombinase-mediated excision of the floxed region enables tissue/cell-specific knockouts of the gene, useful in studies of hematopoietic stem cell maintenance or other CXCL12-dependent processes.

Osx-Cre (Sp7-Cre, see Stock No. 006361)-mediated deletion of Cxcl12 from Sp7 transcription factor 7 (osterix)-expressing stromal cells, which include CXCL12-abundant reticular cells and osteoblasts, results in constitutive hematopoietic progenitor cell (HPC) mobilization and a loss of B-lymphoid progenitors, but HSC function .....
For more information please see the full phenotype on the strain data sheet

020642 B6(SJL)-Slc6a8tm1.1Clar/J
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The CrTflox (also called Slc6a8fl) conditional allele has loxP sites flanking exons 2-4 of the creatine transporter gene on the X chromosome. When bred to mice that express Cre recombinase, the resulting offspring will have deletion of the floxed sequences (encoding the 2nd-4th transmembrane domain of CrT) deleted in the cre-expressing tissues. Homozygous females (CrTflox/flox) and hemizygous males (CrTflox/Y) are viable and fertile with no observed abnormalities.

These CrTflox mice are a Cre-lox tool for generating tissue-specific CrT deletions, and may be useful for studying creatine transport and human X-linked creatine deficiency syndrome, mental retardation, autism, and speech, language, cognitive, and memory disorders.
For example, breeding CrTflox mice to a strain expressing Cre recombinase in embryonic tissues results in offspring with the ubiquitous CrT knockout allele (CrT> .....
For more information please see the full phenotype on the strain data sheet

017591 B6(SJL)-Tardbptm1.1Pcw/J
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These TardbpF mutant mice possess loxP sites flanking exon 3 of the TAR DNA binding protein gene (Tardbp; TDP-43). The Tardbp gene product, TDP-43, is a protein localized to the nucleus of nerve cells. Ubiquinated accumulations of mutant TDP-43 have been linked to the development of Lou Gehrig's disease (Amyotrophic lateral sclerosis; ALS) and frontotemporal lobar degeneration. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.

For example, when bred to B6;129-Gt(ROSA)26Sortm1(cre/ERT)Nat/J (Stock No. 004847), tamoxifen-induced Cre-mediated recombination results in death 9 days after induction in the offspring. These mice exhibit dramatic loss of w .....
For more information please see the full phenotype on the strain data sheet

019520 B6.129(Cg)-Chek1tm1Jmr/J
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Chek1 (checkpoint kinase 1) is a haploinsufficient tumor suppressor gene that is essential for embryonic stem cell survival. CHEK1 null mice reportedly exhibit embryonic lethality at E6.5 due to a peri-implantation defect. Activated CHEK1 prevents cell cycle progression by inhibiting the cell cycle phosphatases Cdc25a and Cdc25c.

Exon 2 of these targeted mutant mice is flanked by loxP sites. When crossed with a tissue-specific Cre strain, the exon is excised, enabling selective disruption of protein production. Homozygous floxed mice are embryonic lethal.

When bred to mice carrying Tg(Wap-cre)11738Mam (see Stock No. 008735 for example), Cre recombinase expression in the mammary gland results in proliferating cells that undergo apoptosis leading to developmental defects. Conditional heterozygosity increases the number of S phase cells and causes spontaneous DNA damage.

This conditional all .....
For more information please see the full phenotype on the strain data sheet

007676 B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
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Mice homozygous for this mT/mG mutation are viable and fertile. These mice possess loxP sites on either side of a membrane-targeted tdTomato (mT) cassette and express strong red fluorescence in all tissues and cell types examined. Tail or whole body epifluorescence is sufficient to identify mT/mG homozygotes. When bred to Cre recombinase expressing mice, the resulting offspring have the mT cassette deleted in the cre expressing tissue(s), allowing expression of the membrane-targeted EGFP (mG) cassette located just downstream. The donating investigator reports that the ACTB promoter allows stronger and persistent expression of the fluorescent proteins (especially in adult cells) compared to the endogenous Gt(ROSA) locus alone. This double-fluorescent system allows direct live visualization of both recombined and non-recombined cells at single cell resolution, offering an internal control for phenotypic analysis of Cre-induced mosaic mutants and providing a second marker fo .....
For more information please see the full phenotype on the strain data sheet
017640 B6.129(Cg)-Nf1tm1Par/J
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Mutation in the human neurofibromin gene, NF1, is the cause of the autosomal dominant disorder Type I Neurofibromatosis. These mice possess loxP sites on either side of exons 31 and 32 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 31 and 32 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in the developing neural tube (see Stock No. 009107 for example), this mutant mouse strain may be useful in studies of Type I Neurofibromatosis.

This allele is also part of the MADM-TG,p53KO,NF1-flox strain (Stock No. 017530), which is a genetic mosaicism model for cancer.

Wh .....
For more information please see the full phenotype on the strain data sheet

021589 B6.129(Cg)-Rab7tm1.1Ale/J
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These Rab7flox mutant mice possess loxP sites flanking exon 1 of the RAB7, member RAS oncogene family (Rab7) gene. RAB7 is a small GTPase that promotes fusion reactions between late endosomes and lysosomes facilitating the lysosomal degradation of plasma membrane proteins, autophagosomes, and endocytic vesicle cargo. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in cre-expressing tissues.

For example, when crossed to a strain expressing Cre recombinase in CD4 T cells (see Stock No. 017336), this mutant mouse strain may be useful for studying the effect of autophagosomes degradation on T cell survival.

004178 B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J
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These Z/EG transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being liver and lung tissue. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with enhanced GFP expression in tissues expressing Cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells.

As an example, when crossed to a strain expressing Cre recombinase in olfactory sensory neurons (see Stock No. 006668), this mutant mouse strain may be useful in lineage tracing.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved t .....
For more information please see the full phenotype on the strain data sheet

016831 B6.129(FVB)-Igf1tm1Dlr/J
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These mutant mice feature loxP sites flanking exon 4 of the insulin-like growth factor 1 (Igf1) gene and a neomycin resistance cassette. Homozygotes for this allele are viable, fertile, and normal in size. IGF1 promotes cell survival and cell proliferation by activating the tyrosine kinase transmembrane receptor, IGF-1R. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in cre-expressing tissues. For example, when these mice are bred to Tg(Alb1-cre)1Dlr/J mice (Stock No. 016832 or Stock No. 016833), IGF1 expression is abolished in liver. This strain may be useful for studying postnatal growth and development.

When bred to a strain expressing Cre recombinase in the early mouse embryo (see Stock No. 003724 for example), this muta .....
For more information please see the full phenotype on the strain data sheet

005247 B6.129(SJL)-Nrp1tm2Ddg/J
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These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.

For example, when bred to B6.Cg-Tg(Tek-cre)1Ywa/J (Stock No. 008863) endothelial cell specific Cre expression results in mice exhibiting abnormal cardiac development and perinatal lethality.

017729 B6.129-Aurkatm1.1Tvd/J
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Aurora kinase A is critical in centrosome separation and bipolar spindle formation, and is involved in the regulation of apoptosis. These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s).

When bred to a strain with tamoxifen-inducible Cre recombinase expression (see Stock No. 004847 for example), this mutant mouse strain may be useful in studies of mitosis and apoptosis.

022009 B6.129-Cflartm1Ywh/J
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These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in thymocytes (see Stock No. 003802 for example), this mutant mouse strain may be useful in studies of T lymphocyte development and apoptosis.

004152 B6.129-Ctnnb1tm2Kem/KnwJ
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These mice possess loxP sites located in introns 1 and 6 of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

When bred to a strain expressing Cre recombinase in chrondocytes (see Stock No. 003554 for example), this mutant mouse strain may be useful in studies of chrondocyte differentiation.

When bred to a strain expressing Cre recombinase in heart(see Stock No. 005650 or 005657 for example), this mutant mouse strain may be useful in studies of cardiovascular disease.

When bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 007807 or > .....
For more information please see the full phenotype on the strain data sheet

021431 B6.129-Elavl1tm1Thla/J
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Elavl1 encodes an RNA-binding protein that binds to AU-rich elements of the 3' UTR of mRNAs. These mice possess loxP sites on either side of exons 2 through 5 of the Elavl1 gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 5 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in the myeloid cell lineage (see Stock No. 004781 for example), this mutant mouse strain may be useful in studies of regulation of post-transcriptional gene expression in angiogenesis.

018063 B6.129-Fth1tm1.1Lck/J
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These mice possess loxP sites flanking the promoter and exon 1 of the Fth1 (ferritin heavy chain 1) gene. Fth1 is part of the ferritin protein complex and is involved in iron storage and iron homeostasis. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have the promoter and exon 1 deleted in the cre-expressing tissues.

When bred to a strain with IFN or pI-pC inducible Cre recombinase expression in liver cells and lymphocytes (see Stock No. 003556 for example), this mutant mouse strain may be useful in studies of iron homeostasis.

008606 B6.129-Gt(ROSA)26Sortm1Joe/J
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Homozygous ROSA26 GNZ knock-in mice are viable and fertile, with a nuclear-localized green fluorescent protein/beta-galactosidase fusion protein (GFP-NLS-lacZ or GNZ) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of GNZ is blocked by an upstream loxP-flanked STOP sequence (in the absence of Cre recombinase, no expressed GFP or beta-galactosidase activity is observed in GNZ embryos(E9.5-18.5)). When bred to cre expressing mice, offspring will have the STOP sequence deleted in tissues where Cre recombinase is present. The resulting GNZ fusion protein expression allows for enhanced (single cell level) visualization / resolution. The donating investigator reports that Cre recombinase activity can be visualized by direct GFP fluorescence, but the high resolution nuclear staining of GNZ may be best visualized by immunostaining for either GFP or beta-galactosidase. These ROSA26 GNZ mice are useful as a Cre reporter strain; expressing both G .....
For more information please see the full phenotype on the strain data sheet
007561 B6.129-Hif1atm3Rsjo/J
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These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s).

For example, when crossed to a strain expressing Cre recombinase in skeletal and cardiac muscle (see Stock No. 006475), this mutant mouse strain may be useful in studies of the metabolic control of muscle function.

When bred to a strain with the targeted null allele in von Hippel-Lindau syndrome homolog, Vhlh (Stock No. 004081) and a strain expressing Cre recombinase in liver (Stock No. 003574), this mutant mouse strain may be use .....
For more information please see the full phenotype on the strain data sheet

023969 B6.129-Kdm1atm1.1Sho/J
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These LSD1fl/fl mice possess loxP sites flanking exons 5-6 of the lysine (K)-specific demethylase 1A (Kdm1a) gene. LSD1 demethylates histone H3 on Lys4 or Lys9 (H3K4/K9) and represses hematopoietic stem and progenitor cell (HSPC) gene expression during hematopoietic differentiation. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 5-6 deleted in the cre-expressing tissues.

For example, when bred to mice expressing Cre-recombinase in hematopoietic cells (see B6.Cg-Tg(Vav1-cre)A2Kio/J Stock No. 008610), double mutant mice die neonatally of severe anemia. Blood counts and bone marrow cellularity are reduced. Also, hematopoietic stem cells, multipotent progenitors, and myeloid progenitor cell numbers decreased 30-fold.

019112 B6.129-Mapk1tm1Gela/J
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These Mapk1flox/flox mutant mice possess loxP sites flanking exon 2 of the mitogen-activated protein kinase 1 (Mapk1) gene. MAPK1, also known as ERK2, is part of the Ras-Raf-ERK pathway, plays a role in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiating cells. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in cre-expressing tissues.

For example, when crossed to FVB-Tg(GFAP-cre)25Mes/J mice (Stock No. 004600) expressing Cre recombinase in the central nervous system, resulting mice behave abnormally and exhibit defect in neuron differentiation and cerebral cortex morphology.

017969 B6.129-Nlrp3tm1Hhf/J
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Nlrp3A350VneoR mice contain a floxed neomycin cassette in intron 2 of the NLR family, pyrin domain containing 3 gene, Nlrp3, in opposite orientation to gene (neoR). These mice also contain a point mutation in exon 3 which results in a missense mutation, A350V, corresponding to human amino acid 352. This mutation is commonly found in humans with Muckle-Wells syndrome (MWS). NLRP3 encodes the protein cryopyrin, which is a cytosolic nucleotide-binding domain and leucine-rich repeat containing (NLR) protein expressed in white blood cells and chondrocytes. Cryopyrin controls the formation of the inflammasome which regulates the immune system's response to injury, toxins, and infection by cleaving interleukin (IL)-1β. NLRP3 mutations are known to cause autoinflammatory diseases known as cryopyrin-associated periodic syndromes (CAPS) such as MWS, familial cold autoinflammatory syndrome (FCAS), and neonatal onset multisystem inflammatory disease (NOMID). Homoz .....
For more information please see the full phenotype on the strain data sheet
004584 B6.129-Ppargtm2Rev/J
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These mice possess loxP sites on either side of exons 1 and 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

When bred to a strain expressing Cre recombinase in adipose tissue (see Stock No. 005069 for example), this mutant mouse strain may be useful in studies of insulin resistance.

When bred to mice carrying Tg(Krt1-15-cre/PGR)22Cot (Stock No. 005249), Cre recombinase expression in the epithelial stem cells results in scarring alopecia.

When bred to mice carrying Tg(Tagln-cre)1Her (Stock No. 017491), Cre recombinase expression in vascular smooth muscle results in pulmonary arterial hypertension (PAH).

When bred to mice carrying Tg(Tek-cre)1Ywa (Stock No. For more information please see the full phenotype on the strain data sheet

009666 B6.129-Ppargc1atm2Brsp/J
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These mice possess loxP sites on either side of exons 3-5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3-5 deleted in the cre-expressing tissue(s).

When bred to a strain expressing Cre recombinase specifically in the liver (see Stock No. 003574 for example), this mutant mouse strain may be useful in studies of hepatic heme biosynthesis and porphyrias.

When bred to a strain expressing Cre recombinase specifically in skeletal muscle, this mutant mouse strain may be useful in studies of neuromuscular junction physiology and muscular dystrophy.

008100 B6.129-Prdm1tm1Clme/J
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These mice possess loxP sites in introns flanking exons 6 to 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 6 to 8 deleted in the cre-expressing tissue(s).

When bred to a strain expressing Cre recombinase during B-lymphocyte development and differentiation (see Stock No. 004126 for example), this mutant mouse strain may be useful in studies of humoral immune response.

When bred to mice carrying Tg(Itgax-cre)1-1Reiz (Stock No. 008068), Cre recombinase expression in dendritic cells results in altered adaptive immune response and lupus-like serology.

022741 B6.129-Tbx21tm2Srnr/J
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These Tbx21F/F mutant mice possess loxP sites flanking exons 2-6 of the T-box 21 (Tbx21) gene. Tbx21 encodes T-bet, a transcription factor that controls gene expression in many cell types of the immune system. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in cre-expressing tissues.

For example, when crossed to STOCK Tg(Cd4-cre)1Cwi/BfluJ mice (Stock No. 017336) expressing Cre recombinase, which deletes Tbx21 during thymic development, the T cell-specific Tbx21 deficient mice exhibit alterations in their response to some infections. CD8+ T cells from LCMV-infected T cell-specific Tbx21 deficient mice have impaired proliferative expansion and increased expression of Il-17, Il-2, and TNF.

018978 B6.129-Tnfsf11tm1.1Caob/J
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These RANKLf/f mutant mice possess loxP sites flanking exons 3-4 of the tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) gene. Tnfsf11 encodes the cytokine RANKL, a ligand for RANK, both of which are essential for osteoclast differentiation, development, function and survival. RANKL is expressed in chondrocytes and osteocytes embedded in the bone matrix where it controls mineralized cartilage resorption, bone remodeling, and bone loss. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3-4 deleted in the cre-expressing tissue(s).

For example, when bred to B6.Cg-Tg(Prrx1-cre)1Cjt/J mice (Stock No. 005584) expressing Cre recombinase in the developing limbs and parts of the skull, double mutant mice had increased bone mineral density and cancello .....
For more information please see the full phenotype on the strain data sheet

016520 B6.129P2(129S4)-Ago2tm1.1Tara/J
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These mice possess loxP sites on either side of exons 9-11 in the Eif2c2 (eukaryotic translation initiation factor 2C, 2) gene, also known as Ago2. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, the Mid domain is deleted and Ago2 expression is lost, causing functional inactivation of the gene.

For example, when crossed to a strain expressing interferon-inducible Cre recombinase (see Stock No. 003556), this mutant mouse strain may be useful in studies of microRNA homeostasis.

017837 B6.129P2(Cg)-Braftm1Mmcm/J
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Mice homozygous for the Cre-activated BRaf mutant allele (BRafCA) are viable and fertile. Mice express the transcript encoded by endogenous exons 1-14 and loxP-flanked human exons 15-18 (BRafCA) prior to Cre recombinase exposure. Homozygous mice have "normal" BRaf expression, and no abnormalities are reported for homozygous BRafCA mice. Following Cre-mediated excision of the floxed sequences (human BRAF exons 15-18 and polyA signal), the transcripts are subsequently generated using the downstream mutant exon 15 (modified with a V600E amino acid substitution associated with constitutively active BRAFV600E in human cancers) and the endogenous downstream exons 16-18. The resulting BRafVE transcripts are subject to normal patterns of alternate splicing and differential exon usage, and BRafVE expression is observed at physiological levels. To discriminate the expression of the various BRaf mRNAs in .....
For more information please see the full phenotype on the strain data sheet
011008 B6.129P2(Cg)-Gt(ROSA)26Sortm1(tTA)Roos/J
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Mice heterozygous for the ROSA:LNL:tTA conditional mutation of the Gt(ROSA)26Sor locus are viable and fertile, with a loxP-flanked STOP cassette preventing transcription of a downstream optimized/modified tetracycline-controlled transactivator protein ("mtTA"). Applying both Cre-lox and Tet-Off technologies, these ROSA:LNL:tTA mutant mice may be useful to generate compound mutant mice in which expression of a tetracycline-responsive promoter element (TRE; also called tet-operator or tetO)-driven gene of interest can be both directed to the cell types defined by the chosen Cre recombinase expression, as well as turned off by the addition of tetracycline (or its analog doxycycline (dox)).
022362 B6.129P2(Cg)-Ntrk1tm1Ddg/J
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Ntrk1 (neurotrophic tyrosine kinase, receptor, type 1; also called TrkA) is a receptor for nerve growth factor (Ngf), a neurotrophin which controls aspects of mammalian nervous system development. NGF-NTRK1 (NGF-TrkA) signaling is crucial for the survival and growth of sympathetic neurons as well as small-diameter nociceptive sensory neurons.

These mice carry a floxed F592A mutation in exon 12 of the mouse Ntrk1 gene. The allele is sensitive to a family of small-molecule inhibitors including 1NaPP1 and 1NMPP1 which act selectively, rapidly and reversibly. Without 1NaPP1 or 1NMPP1 application, homozygous mutant mice are hypomorphic, with an approximate 50% reduction of both the number of calcitonin gene-related peptide (CGRP) and Ret-positive neurons in dorsal root ganglion and a reduction of peripheral innervation of both sensory and sympathetic fibers. Although hypomorphic, the mutants are viable and fertile, and signaling is effectively blocked by applicat .....
For more information please see the full phenotype on the strain data sheet

008888 B6.129P2(SJL)-Myd88tm1Defr/J
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These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s).

When bred to a strain with inducible Cre recombinase expression in dendritic cells (see Stock No. 008068 for example), this mutant mouse strain may be useful in studies of Toll-like receptor signaling during immune responses.

When bred to a strain with Cre recombinase expression in hematopoietic cells (see Stock No. 008610 for example), this mutant mouse strain may be useful in studies of Toll-like receptor signaling and natural killer cells.

When bred to a strain with Cre reco .....
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008767 B6.129P2-Cxcr4tm2Yzo/J
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These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s).

For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 004126, Stock No. 006785), this mutant mouse strain may be useful in studies of B lymphocyte development.

017492 B6.129P2-Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/J
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Mice homozygous for the R26R-Confetti conditional allele are viable and fertile, with a CAG promoter, loxP site, and STOP cassette preventing transcription of the downstream Brainbow 2.1 sequences. The Brainbow 2.1 region contains two loxP-flanked dimers, each uniquely positioned in head-to-tail tandem. One dimer has nuclear-localized green fluorescent protein (hrGFPII) and a reverse-oriented cytoplasmic yellow fluorescent protein (mYFP). The other dimer has cytoplasmic red fluorescent protein (tdimer2(12)) and a reverse-oriented membrane-tethered cyan fluorescent protein (mCerulean). The Brainbow2.1 region may be written as loxP-STOP-loxP-GFP-PFY-Pxol-loxP-RFP-PFC-Pxol to show the transcriptional direction of each part. When bred to mice that express Cre recombinase, the resulting offspring may have a recombination event that stochastically places one of the four fluorescent proteins into position directly downstream of the CAG promote .....
For more information please see the full phenotype on the strain data sheet
009669 B6.129P2-Gt(ROSA)26Sortm1(DTA)Lky/J
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Homozygotes are viable, fertile and do not display any gross physical or behavioral abnormalities. When these ROSA-DTA mice are crossed with a Cre recombinase strain, the floxed-STOP cassette is deleted and the Gt(ROSA)26Sor promoter drives expression of diptheria toxin in the cre-expressing cells. These ROSA-DTA mice allow selective ablation in a tissue/cell-specific manner.

Of note, ROSA-DTA mice are also available on a BALB/c congenic background (see Stock No. 009670).

022409 B6.129P2-Ift88tm1Bky/J
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These Ift88fl mice possess loxP sites flanking exons 4-6 of the intraflagellar transport 88 (Ift88) gene. IFT maintain primary cilia that are present on most cell types, and mediates bidirectional movement of structural and signaling components during events such as development and wound healing. Mutations in cilia-related genes, such as Itf88, have been implicated in many ciliopathic human genetic diseases. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 4-6 deleted in cre-expressing tissues.

For example, when crossed to B6.Cg-Tg(Prrx1-cre)1Cjt/J mice (Stock No. 005584) expressing Cre recombinase in early limb bud mesenchyme, resulting offspring exhibit extensive polydactyly with loss of anteroposterior digit patterning and shortening of the proximodista .....
For more information please see the full phenotype on the strain data sheet

008327 B6.129P2-Leprtm1Rck/J
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These targeted mutation mice possess loxP sites on either side of exon 1. Homozyotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice bearing a Cre recombinase gene under the control of a tissue-specific promoter, exon 1 of the targeted gene is deleted in the tissue of interest. This strain may be useful in studies of obesity.
007177 B6.129P2-Mecp2tm1Bird/J
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These mice possess two functional loxP sites flanking exons 3-4 of the targeted gene on the X chromosome (the donating investigator reports that the middle loxP site is non-functional). Homozygous females and hemizygous males are viable and fertile. Northern blot analysis showed the expected mature transcript from the Mecp2lox locus as well as a transcript in which the beta-globin intron was unspliced. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3-4 deleted in the cre-expressing tissue(s). Mice with this X-linked floxed mutation may be useful in neurological and developmental studies of Rett syndrome.

For example, when crossed to a strain expressing Cre recombinase in nervous tissue (see Stock No. 003771), this mutant mouse strain develops a neurological phenotype that mimics Rett syndrome.

When crossed to a strain expressing Cre .....
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006849 B6.129P2-Mecp2tm2Bird/J
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These mice possess a loxP-flanked STOP cassette in intron 2 of the targeted gene on the X chromosome. Western blot and hybridization analysis confirm the absence of wildtype protein from the targeted allele (although the donating investigator reports that the targeted allele produces a "read-through" transcript which does not give rise to detectable levels of protein but makes it difficult to discriminate between the "flox-stopped" and reactivated alleles by RT-PCR). Hemizygous (Mecp2lox-Stop/y) males do not breed and develop Rett syndrome symptoms (reduced mobility, hindlimb clasping) at approximately 6 weeks of age, with death occurring at approximately 11 weeks of age. Heterozygous females are fertile until developing Rett syndrome characteristics at 4-12 months of age. This Rett syndrome-like phenotype is similar to that observed for the traditional knock-out allele (see Stock No. 003890). Cre recombin .....
For more information please see the full phenotype on the strain data sheet
017309 B6.129P2-Syktm1.2Tara/J
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In this conditional Sykb (spleen tyrosine kinase) mutant strain, exon 1 has been flanked by loxP sites. When crossed with a Cre recombinase-expressing strain, these mice are useful in eliminating tissue-specific expression of the gene. Homozygous floxed mice are fully viable and fertile.

Homozygous null mice show a variety of defects including high rates of perinatal lethality, abnormal vascular morphology, abnormal osteoclast differentiation, impaired neutrophil phagocytosis, and defects in B cell development.

For example,when bred to a strain carrying Tg(Cr2-cre)3Cgn (Stock No. 006368), Cre recombinase expression in mature B cells results in an altered B cell phenotype.

008462 B6.129P2-Trp53tm1Brn/J
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Exons 2-10 are flanked by loxP sites in this conditional targeted mutation. Mice homozygous for the floxed allele do not show any increase in disease incidence for at least a year. When bred to mice with a cre recombinase gene under the control of a promoter of interest, expression is deleted in the tissue of interest.

For example, when crossed to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771), this mutant mouse strain may be useful in studies of medulloblastoma formation.

When crossed to a strain expressing Cre recombinase in the central nervous system (see Stock No. 004600), this mutant mouse strain may be useful in studies of astrocytoma formation.

When crossed to a strain expressing Cre recombinase in virgin and lactating mammary glands (see Stock No. 003553), th .....
For more information please see the full phenotype on the strain data sheet

017641 B6.129S-Mirc30tm1.1Rdf/J
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The Mir15a/Mir16-1 cluster, located in the human 13q14 chromosomal region (conserved in mouse 14qC3), is involved in regulation of B cell proliferation. When deleted, B cell proliferation is accelerated. Chromosomal deletion of human 13q14 is commonly associated with poor prognosis in chronic lymphocytic leukemia (CLL). These mice possess loxP sites on either side of the Mir15a/Mir16-1 cluster. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have Mir15a/Mir16-1 cluster deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in B lymphocytes (see Stock No. 006785 for example), this mutant mouse strain may be useful in studies of chronic lymphocytic leukemia. .....
For more information please see the full phenotype on the strain data sheet

010525 B6.129S-Notch2tm3Grid/J
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These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s).

When bred to a strain with early embryonic Cre recombinase expression (see Stock No. 003755 for example), this mutant mouse strain may be useful in studies of the Notch pathway during development.

When bred to a strain expressing Cre recombinase in embryos, in particular, cardiac neural crest cells (see Stock No. 005549 for example), this mutant mouse strain may be useful in studies of vascular smooth muscle development and the cardiovascular defects associated with Alagille syndrome .....
For more information please see the full phenotype on the strain data sheet

022871 B6.129S-Snai3tm2.1Grid/J
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The Snail gene family encodes zinc finger-containing transcriptional repressor proteins. Snai1 (snail homolog 1 (Drosophila)) and Snai2 (snail homolog 2 (Drosophila)) genes play critical roles during vertebrate embryogenesis as well as tumor progression and metastasis. Comparatively little is known about Snai3 (snail homolog 3 (Drosophila); also known as Smuc), which is predominantly expressed in mouse skeletal muscle and thymus as early as embryonic day 13.5 (E13.5). It is also expressed in heart, lung, and spleen.

This strain carries a targeted knockout of Snai3. Homozygous null mice are viable and fertile and do not exhibit any obvious developmental phenotype. Results suggest this gene is not essential for embryogenesis in mice.

017293 B6.129S1(Cg)-Eomestm1.1Bflu/J
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These Eomesfl/fl mutant mice possess loxP sites flanking exon 2 of the eomesodermin homolog (Eomes) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. EOMES is a member of the T-box family of transcription factors which is expressed in CD8+ T cells, CD4+ T cells, natural killer (NK) cells, and embryonic forebrain floorplate and migrating neuroblasts. EOMES is involved in embryonic development of the central nervous system and mesoderm, and plays a key role in CD44 expression, CD8+ T cell effector function and anticancer responses. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues. This strain may be useful for studying T cell-mediated immune response, immune surveillance and editing, and embryonic development.

For example .....
For more information please see the full phenotype on the strain data sheet

009380 B6.129S1-Irf4tm1Rdf/J
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These mice possess loxP sites on either side of exons 1 and 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 1 and 2 deleted and GFP expression in the cre-expressing tissue(s). When crossed to mice that express Flp recombinase, the entire targeting construct, including exons 1 and 2, are deleted in the FLP expressing tissues.

When bred to a strain expressing Cre recombinase in B lymphocytes (see Stock No. 006785 for example), this mutant mouse strain may be useful in studies of plasma cell development and immunoglobulin class switch recombination.

When bred to a strain expressing Cre recombinase in T regulatory cells, this mutant mouse strain may be useful in studies of autoimmune .....
For more information please see the full phenotype on the strain data sheet

016923 B6.129S1-Stat3tm1Xyfu/J
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These mice possess loxP sites on either side of exons 18, 19 and 20 of the targeted gene. Exons 19 and 20 encode the SH2 domain. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 18, 19 and 20 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in the liver, under the control of the Ttr, transthyretin, promoter, this mutant mouse strain may be useful in studies of liver regeneration.

When bred to a strain with Cre recombinase expression in cardiac tissue (see Stock No. 011037 for example) , this mutant mouse strain may be useful in studies of inflammation-induced cardiac injury.

When bred to a strain with Cre recombinase expression in the bone marro .....
For more information please see the full phenotype on the strain data sheet

018552 B6.129S2(SJL)-Trim28tm1.1Ipc/J
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The Trim28 gene encodes a protein that acts as a transcriptional corepressor, with an important role in chromatin remodeling, genomic imprinting and DNA methylation in pre-implantation development, as well as thymocyte development regulation. These mice possess loxP sites on either side of exons 4 through 14 of the targeted Trim28 gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 4 through 14 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in oocytes (see Stock No. 003651 for example), this mutant mouse strain may be useful in studies of pre-implantation embryonic development.

014090 B6.129S2-Rbfox2tm1.1Dblk/J
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These Fox-2flox mutant mice possess loxP sites flanking exons 6-7 of the RNA binding protein, fox-1 homolog 2 (Rbfox2) gene. Mice that are homozygous for this allele are viable, fertile, and normal in size. Fox-2 is widely expressed, highest in brain and heart, and regulates alternative splicing in vertebrates by binding specifically to (U)GCAUG sequences. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exons 6-7 deleted in the Cre-expressing tissue, resulting in inactivation of Fox-2 gene function.

When bred to mice carrying Tg(Pcp2-cre)2Mpin (Stock No. 004146) and Rbfox1tm1.1Dblk (Stock No. 014089) , Cre recombinase expression in the Purkinje cells may be useful in studies of Purkinje cell pacemaking.

012933 B6.129S4(C)-Vhltm1Jae/J
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This strain contains loxP sites flanking the Vhl promoter and exon 1. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cre-mediated recombination results in the deletion of the promoter and exon 1. Studies in which liver-specific inactivation of the Vhl gene was achieved by breeding this strain with albumin promoter driven-Cre mice (see Stock No. 003574 for example) resulted in hemizygous mice that exhibit cavernous hemangiomas of the liver, a rare component of the human von Hippel-Lindau (VHL) disease.

When bred to a strain expressing Cre recombinase in the myeloid cell lineage (see Stock No. 004781 for example), this mutant mouse strain may be useful in studies of myeloid cell mediated inflammation.

When bred to a strain expressing Cre recombina .....
For more information please see the full phenotype on the strain data sheet

007668 B6.129S4(Cg)-Arntltm1Weit/J
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Mice homozygous for this conditional (floxed) allele possess loxP sites flanking exon 8 of the targeted gene and are viable and fertile, with circadian behavioral rhythms indistinguishable from wildtype littermates. When bred to mice that express Cre recombinase, the resulting offspring will have the exon encoding the ARNTL (BMAL1) basic helix-loop-helix (bHLH) domain deleted in the cre-expressing tissue(s). These Bmal1-floxed mutant mice may be useful in generating conditional mutations (whole-mouse or tissue-specific) to study the role of circadian clock/circadian rhythm in physiological and behavioral regulation.

For example, when crossed to a strain expressing Cre recombinase in the retina (see Stock No. 005105), this mutant mouse strain may be useful in studies of the circadian clock of the retina.

021125 B6.129S4(Cg)-Dcktm1.2Radu/J
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These Dckflox/flox mutant mice possess loxP sites flanking exon 3, encoding the catalytic domain, of the deoxycytidine kinase (Dck) gene. Deoxycytidine kinase is a gene involved in deoxyribonucleoside salvage metabolism for use in DNA replication. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.

For example, when bred to B6.C-Tg(CMV-cre)1Cgn/J mice (Stock No. 006054), resulting Dck deficient offspring exhibit severe defects in T cell, B cell, and erythroid development resulting from acute cell cycle arrest in early S-phase in rapidly proliferating progenitor populations.

020631 B6.129S4(FVB)-Drd2tm1.1Mrub/J
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These Drd2loxP/loxP mice possess loxP sites flanking exon 2 of the dopamine receptor D2 (Drd2) gene. Mice that are homozygous for this allele are viable and fertile. D2 receptors are G-protein coupled receptors located on postsynaptic and presynaptic dopaminergic neurons which inhibit adenylyl cyclase activity. Dopamine (DA) stimulation of presynaptic D2 receptors (autoreceptors) induces a negative feedback regulation that reduces DA neuron firing, DA synthesis and DA release. Defects in DA neurons have been associated with Parkinson's disease, schizophrenia, attention-deficit and hyperactivity disorder, and compulsive drug abuse. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in cre-expressing tissues.

For example, when crossed to B6.SJL-Slc6a3tm1.1(cre)Bkmn/J mice (Stock No. 006660) expressin .....
For more information please see the full phenotype on the strain data sheet

006955 B6.129S4(FVB)-Insrtm1Khn/J
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Mice homozygous for this IRlox allele are viable and fertile. These mutant mice have loxP sites flanking exon 4 of the targeted gene. When bred to Cre-recombinase expressing mice, offspring will have a deletion of exon 4 in the cre expressing tissue(s). These "floxed" mice may be useful in studying insulin receptor function in several different tissues (including pancreas, liver, and skeletal muscle), as well as diabetes and glucose regulation.

For example, when crossed to a strain expressing Cre recombinase in the central and peripheral nervous system (see Stock No. 003771), this mutant mouse strain may be useful in studies of glucose homeostasis.

When crossed to a strain expressing Cre recombinase in skeletal and cardiac muscle (see Stock No. 006475), this mutant mouse strain may be useful in studies of diabetes.

When cr .....
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005246 B6.129S4-Grin1tm2Stl/J
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These floxed NR1 (Grin1flox) mice possess loxP sites flanking approximately 12 kbp of sequence of the targeted gene that encodes the entire transmembrane domain and C-terminal region. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

When bred to a strain expressing Cre recombinase in the CA3 region of the hippocampus (see Stock No. 006474 for example), this mutant mouse strain may be useful in studies of associative memory recall.

When bred to a strain expressing Cre recombinase in the CA1 region of the hippocampus (see Stock No. 005359 for example), this mutant mouse strain may be useful in studies of nonspacial memory.

022367 B6.129S4-Gt(ROSA)26Sortm1(CAG-EGFP/Rpl10a,-birA)Wtp/J
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These fs-TRAP mice contain from 5' to 3', a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a floxed-neomycin (neo) resistance cassette and polyadenylation (polyA) sequence, a frt-flanked enhanced green fluorescence protein (EGFP)-L10a (ribosomal protein) fusion protein, a 2xHA (hemagglutinin) tagged bacterial birA, bifunctional protein (biotin ligase), gene, and a polyA sequence. This targeting vector was inserted into the Gt(ROSA)26Sor gene. Mice that are homozygous for the targeted mutation are viable and fertile. Rpl10a encodes the ribosomal protein L10a, an integral component of the 60S ribosomal subunit.

In the absence of Cre, gene expression is prevented by the STOP sequence. After removal of the loxP-flanked neo-polyA cassette via cre-mediated recombination, constitutively activated EGFP-L10a labels ribosomes of selected tissues where Cre recombinase is expressed. Ribosomes o .....
For more information please see the full phenotype on the strain data sheet

008179 B6.129S4-Krastm4Tyj/J
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This KrasLSL-G12D strain carries a point mutation (G12D) whose expression is blocked by the presence of a loxP-flanked stop codon. Homozygotes die in utero. Cre-mediated recombination can excise the stop codon and permit the oncogenic protein to be expressed. Intranasal infection with an adenovirus encoding Cre results in a very high frequency of lung tumors and permits controlled timing of tumor initiation and tumor multiplicity. This strain may be useful in studies of cancer and development.

When bred to a strain expressing Cre recombinase under the control of a tetracycline-responsive promoter element and a strain expressing a tetracycline-controlled activator protein in lung epithelial cells (see Stock No. 006234 and 006235 respectively), this mutant mouse strain may be useful in studies of lung development.

When bred to a strain expressin .....
For more information please see the full phenotype on the strain data sheet

011009 B6.129S4-Mtortm1.2Koz/J
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Mice homozygous for this mTORfl allele are viable and fertile, with loxP sites flanking exons 1-5 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the putative transcription start site and the coding sequence through coding exon 5 deleted in the cre-expressing tissue(s). These mTORfl mutant mice may be useful in generating conditional mutations for studying mTOR and mTOR complex signaling; including regulation of cell growth, cell proliferation/differentiation, cell survival, cancer, tumor cell motility and metastasis, the RTK-PI3K-mTOR signaling axis, stem cell development, cytokine signaling, and T cell lineage commitment.

For example, when bred to a strain expressing Cre recombinase in adult striated muscle fibers and embryonic striated muscle cells of the somites and heart (see Stock No. 006149 for example), .....
For more information please see the full phenotype on the strain data sheet

018433 B6.129S4-Pdgfratm12Sor/J
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The PDGFRα(S)K conditional knockin allele has the endogenous PDGFRα sequences replaced with a loxP-flanked PGKneo (lox-stop-lox) cassette upstream of the constitutively active PDGFRαK mutant isoform. This D842V mutation in the kinase domain interferes with the inactive conformation of the ATP-binding pocket leading to constitutive PDGFRα activity. Prior to Cre recombinase exposure, no expression of the constitutively active αK mutant isoform is observed, and the mutant allele functions as a knockout. Mice homozygous for the PDGFRα(S)K allele die during embryonic development. Heterozygous PDGFRα(S)K mice are viable and fertile with no reported abnormalities. Following Cre recombinase-mediated excision of the floxed-STOP cassette, high basal kinase activity without the addition of ligand is observed in the cre-expressing tissues.

Of note, pan-deletion of the floxed-STOP cassette during develo .....
For more information please see the full phenotype on the strain data sheet

006440 B6.129S4-Ptentm1Hwu/J
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These mice possess loxP sites flanking exon 5 of the targeted gene. Mice homozygous for the "floxed" allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.

For example, when crossed to a strain expressing Cre recombinase in astrocytes (see Stock No. 012887), this mutant mouse strain may be useful in studies of neurogenesis.

When crossed to a strain expressing Cre recombinase in the central nervous system (see Stock No. 004600), this mutant mouse strain may be useful in studies of glia differentiation and cerebellar development.

When crossed to B6.129X1-Twist2tm1.1(cre)Dor/J mice (Stock No. For more information please see the full phenotype on the strain data sheet

019141 B6.129S6(FVB)-S1pr1tm2.1Rlp/J
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These S1pr1loxP/loxP mice possess loxP sites flanking exon 2 of the sphingosine-1-phosphate receptor 1 (S1pr1) gene. S1PR1 is a G protein-coupled receptor for lysophospholipid sphingosine-1-phosphate (S1P) and is highly expressed in endothelial cells. S1PR1 is essential for vascular maturation during embryonic development and is also involved in cell survival, migration, adhesion, and proliferation. This receptor plays a role in the regulation of innate and adaptive immune responses by controlling lymphocyte egress from the thymus, spleen, bone marrow, and lymph nodes. It has also been implicated in the regulation of vascular function. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in cre-expressing tissues.

For example, when crossed to B6.Cg-Tg(Tek-cre)1Ywa/J (Stock No. For more information please see the full phenotype on the strain data sheet

007611 B6.129S6(SJL)-Cdh2tm1Glr/J
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These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissue(s).

When bred to a strain with inducible Cre recombinase expression in cardiac cells (see Stock No. 005657 for example), this mutant mouse strain may be useful in studies of myocardium physiology.

021301 B6.129S6-Nlrp1btm1Bhk/J
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In this strain, a loxP-flanked neo cassette replaces exons 1-3 of the NLR family, pyrin domain containing 1B (Nlrp1b) gene, abolishing expression. Of note, these mice were developed on a 129 background. 129 mice do not encode functional Nlrp1a and Nlrp1c proteins, therefore, inactivation of the functional Nlrp1b gene is expected to yield an Nlrp1-/- colony. Nlrp1b encodes a pattern recognition receptor that is involved in the formation of the inflammasome which regulates the immune system's response to injury, toxins, and infection by cleaving interleukin (IL)-1β. Nlrp1b is specifically activated upon sensing the anthrax lethal toxin and the resulting release of pro-inflammatory cytokines aids in the clearance of the anthrax infection. Homozygous Nlrp3-deficient mice are viable and fertile. Bone marrow derived macrophages from these Nlrp1b-/- mice are not sensitive to anthrax LT challenge. These mice are able to f .....
For more information please see the full phenotype on the strain data sheet
012914 B6.129S6-Nr3c1tm2.1Ljm/J
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These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in the T cell lineage (see Stock No. 003802 for example), this mutant mouse strain may be useful in studies of hypothalamic-pituitary-adrenal axis homeostasis.

008681 B6.129S7-Atoh1tm3Hzo/J
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These mice possess loxP sites on either side of the coding region of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in further elucidating the role of this gene in cell fate commitment.
008039 B6.129S7-Gja1tm1Dlg/J
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Mice homozygous for this Gja1tm1Dlg (also known as Cx43flox) conditional allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. Presence of the loxP sites has no reported affect on expression of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have exon 2 deleted in the cre-expressing tissue(s). These Gja1tm1Dlg mutant mice may be useful in generating conditional mutations for studying the role of connexin and gap junctions in various tissues and systems, including the cardiovascular system.
013106 B6.129S7-Sox9tm2Crm/J
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These Sox9flox mutant mice possess a loxP site upstream of exon 2, a neomycin resistance (neo) cassette followed by another loxP site downstream of exon 3 of the SRY-box containing gene 9 gene, Sox9. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exons 2-3 deleted in the cre-expressing tissue, resulting in inactivation of Sox9 gene function. Breeding these Sox9flox mice to strains that express Cre recombinase ubiquitously results in severe bone defects and perilethality. This strain may be useful for studying cell fate determination including endochondral bone formation, limb development and patterning, joint formation, and hair and stem cell differentiation
006148 B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J
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Mice that are homozygous for the R26-stop-EYFP mutant allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice contain an Enhanced Yellow Fluorescent Protein gene (EYFP) inserted into the Gt(ROSA)26Sor locus. Expression of EYFP is blocked by an upstream loxP-flanked STOP sequence. When bred to mice with a cre recombinase gene under the control of a promoter of interest, the STOP sequence of the targeted gene is deleted in the tissue of interest, and EYFP expression is observed. These mutant mice may be useful in monitoring the Cre expression in living tissues, and tracing the lineage of such cells in embryos, young, and adult mice at desired time points.

These R26-stop-EYFP have also been used as a fluorescent reporter by the Allen Institute for Brain Science. For their characterization information, see images at the Allen Institute for Brain Science website (> .....
For more information please see the full phenotype on the strain data sheet

013181 B6.129X1-H2-Ab1tm1Koni/J
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These iabneo floxed mutant mice possess a loxP-flanked neomycin resistance (neo) cassette upstream of the exon 1, and a single loxP site downstream of exon 1 of the histocompatibility 2, class II (MHC-II) antigen, beta 1 (H2-Ab1) locus. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, some of the resulting offspring will have exon 1 deleted in cre-expressing tissues. When bred with mice expressing Cre-recombinase driven by a cd19 promoter, iab deficiency results in the loss of MHC-II from the surface most B cells. This results in severely impaired T cell-dependent primary B cell antibody response upon immunization while exhibiting delayed kinetics in B cell germinal centers and memory B cell development. When bred with mice expressing Cre-recombinase driven by endothel .....
For more information please see the full phenotype on the strain data sheet
007181 B6.129X1-Notch1tm2Rko/GridJ
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Mice homozygous for this "floxed" Notch1 allele (fN1) are viable and fertile. These mice possess loxP sites on either side of exon 1 of the targeted gene. When bred to mice with a Cre recombinase gene, exon 1 of the targeted gene is deleted in the cre expressing tissue(s). These conditional knockout mice may be useful in generating tissue-specific mutants for studying the development of a wide range of tissues: for example, when crossed to a strain expressing Cre recombinase primarily in the nervous system (see Stock No. 003771), this mutant strain may be useful in studies of apoptosis in neural development.

When crossed to a strain expressing a differential Cre mediated reporter protein labeling: Notch1 signaling in actively cycling stem/progenitor cells (see Stock No. 006953), this mutant strain may be useful in studies of loss of Notch1 heterozyg .....
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021067 B6.Cg-Abca1tm1Jp Abcg1tm1Tall/J
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These Abca1fl/fl Abcg1fl/fl double floxed mice possess loxP sites flanking exons 45-46 of the ATP-binding cassette, sub-family A (ABC1), member 1 (Abca1) gene and loxP sites flanking exon 3 of the ATP-binding cassette, sub-family G (WHITE), member 1 (Abcg1) gene. Two loxP-flanked neomycin resistance cassettes are still present in these mice, and the donating investigator reports that they do not effect protein expression in macrophages. ABCA1 controls the assembly of phospholipids and free cholesterol with lipid-free Apolipoprotein A-I (apoA-I) to form high density lipoprotein (HDL) and also mediates cholesterol efflux to apolipoproteins and small, pre-beta HDL particles from peripheral cells. Mutations in Abca1 are associated with Tangier disease and familial hypoalphalipoproteinemia, which are characterized by near absence of plasma HDL and the accumulation of cholesterol esters in tissues. ABCG1 is involved in .....
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013590 B6.Cg-Braftm1Mmcm Ptentm1Hwu Tg(Tyr-cre/ERT2)13Bos/BosJ
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Mice that are hemizygous for the Tg(Tyr-cre/ERT2)13Bos transgene, heterozygous for the Braftm1Mmcm allele and homozygous for the floxed Ptentm1Hwu allele are viable and fertile, but 25% will develop melanomas with an onset of 2 to 12 months of age. Topical application of tamoxifen results in development of pigmented skin lesions within 21-28 days, and which progress to malignant melanoma. Metastases are detected in lymph nodes and lung. All treated mutant mice developed metastases in regional draining lymph nodes.

The Donating Investigator reports that by 6 months of age, mice hemizygous for the Tg(Tyr-cre/ERT2)13Bos transgene, heterozygous for the Braftm1Mmcm allele and homozygous for the floxed Ptentm1Hwu allele, exhibit a 25-60% spontaneous melanoma rate in the absence of 4-hydroxytamoxifen induction, due to "leaky" Cre recombinase expression and activity in a genetic background setting that is condu .....
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016849 B6.Cg-Ccl2tm1.1Pame/J
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These Ccl2-RFPlox/lox mice contain loxP sites flanking exons 2-3 of the chemokine (C-C motif) ligand 2 (Ccl2 or Mcp1) gene, as well as an HA peptide followed by an aphthovirus 2A cleavage site and a cleavable red fluorescent protein (mcherry) at the 3' end of exon 3. Homozygous mice are viable and fertile. MCP1 is a chemokine required for the movement of monocytes from bone marrow into the blood stream during their recruitment to sites of injury and infection. Fluorescence is seen in all MCP1 expressing cells. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2-3, as well as RFP expression, deleted in the cre-expressing tissue. Deletion of MCP1 results in decreased monocyte emigration from the bone marrow upon LPS stimulation and increased susceptibility to infection. These mice may be useful for studying emigration of inflammatory monocytes from the bone marrow after infection.

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021212 B6.Cg-Csf1rtm1Jwp/J
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These Csf1rfl/fl floxed mutant mice possess loxP sites flanking exon 5 of the colony stimulating factor 1 receptor (Csf1r) gene. A neo cassette followed by a third loxP site is still present downstream of floxed gene. The donating investigator reports that the presence of the neo cassette in the Csf1r gene does not significantly interrupt gene transcription. Csf1r encodes Cd115 which acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. Expressed in macrophages, expression is also seen in oocytes, osteoclasts, trophoblasts, CNS microglia, and some myoblasts. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 5 and the neo cassette deleted in cre-expressing tissues.

For example, when crossed to B6.Cg-Tg(Nes-cre) .....
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006366 B6.Cg-Dicer1tm1Bdh/J
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These mice contain loxP sites on either side of exon 23. Homozygous mice are viable and fertile with no gross phenotypic or behavioral abnormalities. Expression of the targeted allele is indistinguishable from wild-type despite the frt-flanked neomycin cassette. Cre-mediated recombination (resulting in deletion of exon 23) in the germline leads to developmental arrest at embryonic day 7.5 (E7.5). Tissue specific deletion has been shown to result in loss of microRNA (miRNA) processing. Mutant mice can be used to generate cell/tissue-specific deletions of the endogenous gene for applications in embryonic development, translation, protein processing and miRNA/siRNA regulation of gene expression.

For example, when crossed to a strain expressing Cre recombinase in mesenchyme (see Stock No. 005584), this mutant mouse strain may be useful in studies of limb morphogenesis.

When bred to a strain expressing Cre recombi .....
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021071 B6.Cg-Gt(ROSA)26Sortm1(CAG-PA-GFP)Rmpl/J
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Mice heterozygous for the R26 PA-GFP::NLS allele are viable and fertile. The R26 PA-GFP::NLS allele contains a CAG promoter, loxP-flanked neomycin-β-galactosidase fusion protein (βgeo) with a STOP codon, and a photoactivatable green fluorescent protein (PA-GFP) fused to a nuclear localization signal (NLS). This construct was inserted into the endogenous Gt(ROSA)26Sor locus. In the absence of Cre, PA-GFP expression is prevented by the βgeo sequence. After removal of the loxP-flanked cassette via cre-mediated recombination, PA-GFP is expressed in all cells where Cre recombinase is expressed. PA-GFP::NLS is enriched in the nucleus, but can be also detected in the cytoplasm of neuronal cells. PA-GFP shows very weak fluorescence at 517nm when excited at 488nm before photoactivation. Upon photoactivation with light at ~400nm, PA-GFP is converted into its fluorescent form. This form shows strong emission at 517nm when excited .....
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005670 B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
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Homozygous mutant mice are viable, fertile, normal in size and do not display any behavioral abnormalities. When these gene targeted mice are bred to transgenic strains expressing Cre recombinase, functional rtTA and EGFP activity is observed in the double mutant offspring in the tissues that express cre. These double mutant mice may be bred to transgenic strains carrying genes of interest under the regulation of tetracycline responsive elements (TRE; tetO) to generate triple mutant mice in which the tissue specificity of the cre-transgenic line and doxycycline inducibility of the rtTA/TRE-controlled transgenes can be combined to regulate expression of the target gene.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify th .....
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007914 B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J
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Ai14 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE::deltaNeo conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. Ai14 mice are not expected to express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre is expected to be detectable by fluorescence and mRNA (in situ hybridization). These Ai14 mice are useful as a Cre reporter strain; expressing the red fluorescent protein variant, tdTomato, following Cre-mediated recombination.

For cha .....
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012567 B6.Cg-Gt(ROSA)26Sortm27.1(CAG-COP4*H134R/tdTomato)Hze/J
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Ai27D (or Ai27Δneo) mice heterozygous for the Rosa-CAG-LSL-hChR2(H134R)-tdTomato-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream hChR2(H134R)-tdTomato fusion gene. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, hChR2(H134R)-tdTomato expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the hChR2(H134R)-tdTomato fusion protein. The donating investigator reports that Ai27D mice do not express hChR2(H134R)-tdTomato prior to introduction of Cre recombinase. Fusion protein expression following exposure to cre can be detected by tdTomato fluorescence and mRNA (in situ hybridization) (and presumably by antibody staining (immunohistochemistry); althoug .....
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007903 B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J
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Ai3 mice hemizygous for this Rosa-CAG-LSL-EYFP-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream enhanced yellow fluorescent protein (EYFP). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of EYFP. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, EYFP expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai3 mice do not express EYFP prior to introduction of Cre recombinase and EYFP expression following exposure to cre can be detected by fluorescence, mRNA (in situ hybridization) and antibody staining (immunohistochemistry). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-medi .....
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021188 B6.Cg-Gt(ROSA)26Sortm40.1(CAG-aop3/EGFP)Hze/J
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Ai40Δneo (Ai40D) mice heterozygous for the Rosa-CAG-LSL-ArchT-EGFP-WPRE-bGHpA conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream ArchT-EGFP fusion gene (see below for detailed description of ArchT-EGFP). Because the CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, ArchT-EGFP expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the ArchT-EGFP fusion protein.
The donating investigator reports that Ai40D mice do not express ArchT-EGFP prior to introduction of Cre recombinase. Fusion protein expression following exposure to cre can be detected by native EGFP fluorescence and in situ hybridization (using an EGFP probe) [and presumably by antibody staining ( .....
For more information please see the full phenotype on the strain data sheet
007906 B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J
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Ai6 mice hemizygous for this Rosa-CAG-LSL-ZsGreen1-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream enhanced green fluorescent protein (ZsGreen1). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of ZsGreen1. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, ZsGreen1 expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai6 mice do not express ZsGreen1 prior to introduction of Cre recombinase and ZsGreen1 expression following exposure to cre can be detected by fluorescence and mRNA (in situ hybridization). Bright fluorescence is observed mainly in cell bodies. Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the report .....
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007909 B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
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Ai9 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai9 mice do not express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre can be detected by fluorescence and mRNA (in situ hybridization). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-mediated recombination if .....
For more information please see the full phenotype on the strain data sheet
022625 B6.Cg-Hdac2tm1.1Rdp/J
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Chromatin remodeling, especially through histone-tail acetylation, is emerging as a fundamental mechanism for regulating gene expression. Inhibition of histone deacetylases increases histone-tail acetylation and facilitates learning and memory in wildtype mice as well as in mouse models of neurodegeneration. HDAC2 (histone deacetylase 2) deficiency results in increased synapse number and memory facilitation.

These mice carry a floxed allele of the Hdac2 gene. Exons 5 and 6, encoding the HDAC-catalytic core of the protein, are flanked by loxP sites. Western blot analysis demonstrates that HDAC2 expression in floxed mice is similar to that of wildtype. Cre-mediated excision of the floxed segment results in a null genotype, lacking protein expression.

Deletion of the protein in the germline using EIIa-cre or nestin-cre transgenic mice results in viable and fertile heterozyous mice with no obvious histological abnormalities up to a year of age. Homozygous knockout mice a .....
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022731 B6.Cg-Igs7tm62.1(tetO-tdTomato)Hze/J
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These Ai62(TITL-tdT)-D mice are a fluorescent Cre/Tet reporter line utilizing an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated (TIGRE). Ai62(TITL-tdT)-D mice harbor the TIGRE-Ins-TRE-LSL-tdTomato::deltaHygro conditional allele designed with a modified Tet response element (TRE or tetO) and loxP-flanked STOP cassette upstream of the tdTomato fluorescent protein. When bred with other mice expressing Cre recombinase, tetracycline-controlled transactivator protein (tTA) and/or reverse tetracycline-controlled transactivator protein (rtTA), tdTomato expression in cells/tissues where the expression patterns of the individual promoters driving Cre recombinase and tTA/rtTA overlap can be regulated with tetracycline or its analog doxycycline (dox). Heterozygous mice are viable and fertile with no reported gross physical or behavioral abnormalities. The donating investigator has not attempted to generate homozygous mice to date (Jun .....
For more information please see the full phenotype on the strain data sheet
021021 B6.Cg-Nr3c1tm1.1Jda/J
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These GRfl/fl mutant mice possess loxP sites flanking exon 3 of the nuclear receptor subfamily 3, group C, member 1 (Nr3c1) gene. Nr3c1 encodes the glucocorticoid receptor which regulates genes involved in development, metabolism, and immune response by binding to cortisol and other steroid hormones. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues. Widespread deletion of GR results in perinatal lethality due to failure to produce surfactant.

When bred to B6.Cg-Tg(Lck-cre)548Jxm/J mice (Stock No. 003802) expressing thymocyte-specific cre, the resulting offspring exhibit compromised immune responses due to altered selection of the antigen specific TCR repertoire.

006492 B6.Cg-Pdgfratm8Sor/EiJ
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These mice possess loxP sites on either side of exon 1 and exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.

For example, when bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 007807 or 009107 for example), this mutant mouse strain may be useful in studies of cranial and cardiac neural crest cells.

013188 B6.Cg-Rptortm1.1Dmsa/J
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These floxed mutant mice possess loxP sites flanking exon 6 of the targeted Rptor (regulatory associated protein of MTOR, complex 1) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to C57BL6-Tg(AlbCre)21Mgn (Stock No. 003574) mice, which direct liver-specific expression of Cre, mice exhibit a decrease in liver size yet produce ketones for hours after feeding. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 6 deleted in the cre-expressing tissues. This strain may be useful for studying mTOR (mammalian or mechanistic target of rapamycin)-dependent regulation of ketogenesis and other cellular processes in response to feeding and fasting.
017008 B6.Cg-Smad7tm1.1Ink/J
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SMAD7 is an inhibitor of transforming growth factor-β which regulates naive T cell differentiation and inflammatory cellular responses. Smad7fl/fl mutant mice possess loxP sites flanking exon 1 of the MAD homolog 7 (Smad7) gene. Homozygotes are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 1 deleted in cre-expressing tissues.

For example, when crossed to a strain expressing CD4-Cre recombinase (Stock No. 017336), this mutant mouse strain exhibits a lower frequency of activated CD4+ and CD4+ cells, and less inflammation, demyelination, and axonal damage.

007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J
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These Thy1-Brainbow 1.0 (line H) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Prior to Cre-mediated recombination, the fluorescent protein immediately adjacent to the promoter, tdimer2(12) (RFP), is expressed in peripheral and central neurons. When bred to Cre recombinase expressing mice, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): tdimer2(12) (RFP) (no recombination), mYFP, or mCerulean (CFP). A palmitoylation sequence tethers the mYFP and mCerulean (CFP) to the membrane, allowing clear labeling of axonal processes, while cytoplasmic tdimer2(12) (RFP) better labeled neuronal cell bodies and dendrites. Integration of tandem transgen .....
For more information please see the full phenotype on the strain data sheet
015805 B6.Cg-Tg(UBC-GFP,-TVA)1Clc/J
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The cTVA transgene contains the human ubiquitin C (UBC) promoter/enhancer elements drive expression of a loxP-flanked green fluorescent protein (GFP) and polyadenylation sequence, followed by an avian specific retroviral receptor (TVA) gene derived from quail. Hemizygous mice are viable and fertile. UBC is a polyubiquitin precursor responsible for the regulation of cell signaling pathways upon conjugation with various proteins. In this mutant mouse, GFP is expressed in UBC-expressing cells. When bred to mice that express Cre recombinase, offspring will have the floxed-GFP-STOP cassette deleted in the cre-expressing tissue(s), resulting in TVA overexpression in UBC-expressing cells. Upon expression of TVA, these cells are capable of binding viruses with the envelope protein of avian sarcoma/leukosis virus subtype A (ASLV-A). These cTVA mice may be useful for infection by retroviruses, rabies virus, and other viruses with ASLV-A .....
For more information please see the full phenotype on the strain data sheet
017563 B6;129-Fbxw7tm1Iaai/J
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These Fbw7f/f mice possess loxP sites flanking exons 5 and 6. These exons encode the F-box domain required for ubiquitination complex assembly. As a ubiquitin ligase, Fbwx7 is involved in targeting the cell cycle regulators c-Myc and Notch1 for proteasomal degradation. Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in generating conditional mutations to study regulation of hematopoietic stem cells and tumor suppression.

For example, when crossed to a strain expressing interferon-inducible Cre recombinase (see Stock No. 003556), mice exhibit impaired hematopoietic stem cell self-renewal and differentiation.

023729 B6;129-Fgf10tm1.2Sms/J
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These Fgf10c mice possess loxP sites flanking exon 2 of the fibroblast growth factor 10 (Fgf10) gene. The donating investigator reports that a tetracycline response element present upstream of exon 2 has no effect on expression unless a tetO binding protein is present and activated. FGF10 is expressed in head and limb mesenchyme, and is involved in cell division, embryonic development, regulation of cell growth and maturation, formation of blood vessels, and wound healing. Homozygotes are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

For example, when bred to BALB/c-Tg(CMV-cre)1Cgn/J mice (Stock No. 003465), FGF10 null mice fail to initiate inner ear and limb development.

024179 B6;129-Gt(ROSA)26Sortm1(Actb-T,-GFP)Dalco/J
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Chordomas are malignant tumors that originate from remnants of the notochord, often arising at the base of the skull and along the spine. Duplication mutations of the human brachyury gene (T) have been identified in 4 unrelated families with chordomas. These targeted mutation mice carry a floxed STOP cassette followed by a murine brachyury (T) cDNA and IRES-GFP sequence inserted into the Gt(ROSA)26Sor locus. Cre mediated excision of the flox-STOP cassette results in expression of the chimeric protein. Mice that are homozygous for the targeted mutation are viable and fertile.
010527 B6;129-Gt(ROSA)26Sortm1(DTA)Mrc/J
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These R26RDTA (or ROSA26-DTA176) mice carry a loxP-flanked stop cassette associated with an attentuated diptheria toxin. When crossed with a Cre recombinase-expressing strain, tissue/cell-specific ablation of cells can be achieved. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
004077 B6;129-Gt(ROSA)26Sortm2Sho/J
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These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted into the Gt(ROSA)26Sor locus. Expression of the EGFP gene is blocked by a loxP-flanked STOP fragment placed between the EGFP sequence and the Gt(ROSA)26Sor promoter. This strain serves as a reporter strain, with successful Cre excision being indicated by EGFP expression in cre-expressing tissues. Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The donating investigator indicates that the EGFP expression level in this reporter strain is suitable for applications involving FACS but is too low for histological applications.
012251 B6;129-Igf1rtm2Arge/J
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These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s).

When crossed with a mouse overexpressing constitutively active Kras and expressing mammary gland specific Cre recombinase, this IGF1RLox mutant mouse strain may be useful in studies of mammary tumorigenesis (Proc Natl Acad Sci U S A 2009 Feb 17;106(7):2359-64).

When bred to a strain with Cre recombinase expression in the CNS, this mutant mouse strain may be useful in studies of myelination (Genesis 2000 Feb;26(2):133-5).

When bred to a strain carrying Tg(BGLAP-cre)1Clem (Stock No. 019509), Cre recombinase .....
For more information please see the full phenotype on the strain data sheet

004605 B6;129-Itgb1tm1Efu/J
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These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

When bred to a strain expressing Cre recombinase in the epithelial cells of the intestine (see Stock No. 004586 for example), this mutant mouse strain may be useful in studies of intestinal hyperplasia.

When bred to a strain expressing Cre recombinase in the podocytes of the kidney glomeruli (see Stock No. 008205 for example), this mutant mouse strain may be useful in studies of glomerular structural integrity.

022732 B6;129-Palb2tm1.1Dli/J
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These Palb2fl/fl mutant mice possess loxP sites flanking exons 2-3 of the partner and localizer of BRCA2 (Palb2) gene. PALB2 binds to and colocalizes with the breast cancer 2 early onset protein (BRCA2) in the nucleus where they act to repair double strand DNA breaks and suppress tumor formation. Palb2 deficiency has been associated with familial predisposition to breast and pancreatic cancers. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 2-3 deleted in the cre-expressing tissues.

For example, when bred to STOCK Tg(KRT14-cre)1Amc/J mice (Stock No. 004782) expressing Cre recombinase in the basal epithelium of the mammary ducts, skin, and oral mucosa, Palb2 deficiency results in mammary tumor formation with a long latency of 420 day. When these Palb2<> .....
For more information please see the full phenotype on the strain data sheet

018120 B6;129-Pitx2tm1.1Sac/J
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These Pitx2flox mice possess loxP sites flanking exon 4 of the paired-like homeodomain transcription factor 2 (Pitx2) gene. PITX2 is a transcription factor expressed in the many tissues including the hypothalamus, eye, mandible, pituitary gland, and heart. PITX2 is involved in memory processing and regulating neural axonal migration. Mutations in PITX2 have been associated with Rieger syndrome, a haploinsufficiency disorder affecting the eyes and teeth. Homozygotes are viable, fertile, and normal in size. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in cre-expressing tissues.

For example, when bred to B6.Cg-Tg(Nes-cre)1Kln/J (Stock No. 003771) expressing Cre in the nervous system, the resulting offspring display an abnormal development of mammillothalamic tracts.

004293 B6;129-Shhtm2Amc/J
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Mice that are homozygous for the Shhtm2Amc targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This conditional mutant contains two loxP sites flanking exon 2 of the targeted allele. Cre-mediated recombination excises exon 2 and some surrounding intronic sequence, generating a null allele. When the conditional mutant is crossed with a ubiquitously-expressing Cre recombinase carrier to remove Shh activity in the early embryo, the resulting phenotype resembles the Shh null mutation. These conditional mutant mice may be mated to strains expressing Cre recombinase to study the effects of temporal and tissue-specific ablation of the targeted allele. This mutant mouse strain represents a model that may be useful in studies of developmental defects resulting from disruption of Shh-dependent pathways.

When bred to a strain expressing Cre recombinase under the control of a tet .....
For more information please see the full phenotype on the strain data sheet

008041 B6;129-Sirt1tm1Ygu/J
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Mice homozygous for this targeted allele (SirT1co/co) are viable and fertile. A loxP-flanked neomycin cassette just upstream of exon 4 and a third loxP site downstream of exon 4 were inserted to create this targeted mutant Sirt1 allele. The floxed mutation does not affect SIRT1 protein expression in MEFs or mammary gland tissue in homozygotes. When bred to mice that express Cre recombinase, the resulting offspring have exon 4 (encoding an evolutionarily conserved Sir2 motif) deleted in cre-expressing tissue(s); (the donating investigator reports only one recombination event: complete removal of the neomycin cassette and exon 4, leaving a single loxp). These SirT1co/co mice may be useful in generating conditional mutants for studying transcriptional regulation and the role of estrogen, insulin growth factor-1 (IGF-1), and transcription factors (including NF-kappaB) in mammary gland development, mammary cancer, apoptosis, and metabolic di .....
For more information please see the full phenotype on the strain data sheet
012603 B6;129-Tgfbr2tm1Karl/J
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These TβRII floxed mutant mice possess loxP sites flanking exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in cre-expressing tissues. This strain may be useful for studying the cellular and mechanical role of TGF-β in regulating development, hematopoiesis, wound healing, and immune function.

For example, when crossed to a strain expressing Cre recombinase in the neural tube, midbrain and dorsal spinal cord (see Stock No. 007807), this mutant mouse strain may be useful in studies of DiGeorge syndrome.

For example, when crossed to a strain expressing interferon inducible Cre recombinase (see Stock No. > .....
For more information please see the full phenotype on the strain data sheet

016847 B6;129-Txniptm1Rlee/J
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Exon 1 of this Txnip (thioredoxin interacting protein) targeted mutation strain is flanked by loxP sites. When bred to mice expressing cre recombinase under the control of a promoter of interest, selective excision of the floxed exon can be achieved, knocking out expression in a tissue/cell-specific manner. Homozygous floxed mice are viable and fertile.

For example, when crossed to a strain expressing tamoxifen-inducible Cre recombinase in heart tissue (see Stock No. 005657), this mutant mouse strain may be useful in studies of myocardial energy metabolism.

017524 B6;129-Tg(CMV-Bgeo,-WGA,-ALPP)1Mgmj/J
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These transgenic mice express a Cre recombinase-inducible wheat germ agglutinin lectin (WGA), under the direction of the CMV, cytomegalovirus, promoter. This strain serves as a reporter strain, with successful Cre excision being indicated by WGA expression in cre-expressing neurons. WGA acts as a trans-synaptic tracer, making it possible to identify the synaptic contacts of cre-expressing neurons. Alkaline phosphatase activity is not detected in the CNS. Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. Transgene expression in the iZ/WAP strain is more widespread throughout the CNS when compared to the similar strain, B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J (Stock No. 006465).
006847 B6;129P2-Mecp2tm1Bird/J
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These mice possess two functional loxP sites flanking exons 3-4 of the targeted gene on the X chromosome. Homozygous females and hemizygous males are viable and fertile. Northern blot analysis showed the expected mature transcript from the Mecp2lox locus. Also detected was an unspliced beta-globin transcript that was introduced into the locus as part of the targeting vector. When these mutant mice are bred to mice that express cre recombinase, resulting offspring will have exons 3-4 deleted in the cre-expressing tissue(s). Mice with this X-linked floxed mutation may be useful in neurological and developmental studies of Rett syndrome.

For example, when crossed to a strain expressing Cre recombinase in nervous tissue (see Stock No. 003771), this mutant mouse strain develops a neurological phenotype that mimics Rett syndrome.

When bred to a strain expressing Cre recombinase in embryonic fo .....
For more information please see the full phenotype on the strain data sheet

012569 B6;129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J
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Ai32 mice homozygous for the Rosa-CAG-LSL-ChR2(H134R)-EYFP-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream ChR2(H134R)-EYFP fusion gene. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, ChR2(H134R)-EYFP expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the ChR2(H134R)-EYFP fusion protein. The donating investigator reports that Ai32 mice do not express ChR2(H134R)-EYFP prior to introduction of Cre recombinase. Fusion protein expression following exposure to cre can be detected by EYFP fluorescence (and presumably by mRNA (in situ hybridization) and antibody staining (immunohistochemistry); although this was not tested by the donating investiga .....
For more information please see the full phenotype on the strain data sheet
012570 B6;129S-Gt(ROSA)26Sortm34.1(CAG-Syp/tdTomato)Hze/J
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Ai34D (or Ai34Δneo) mice heterozygous for the Rosa-CAG-LSL-Synaptophysin-tdTomato-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream Synaptophysin-tdTomato fusion gene. The Synaptophysin-tdTomato fusion protein is composed of near-full-length mouse synaptophysin protein fused in-frame to the amino terminus of the tdTomato fluorescent protein. Because the CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, Synaptophysin-tdTomato expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the Synaptophysin-tdTomato fusion protein. The donating investigator reports that Ai34D mice do not express Synaptophysin-tdTomato prior to introduction of Cre recombinase. Following exposure to .....
For more information please see the full phenotype on the strain data sheet
012735 B6;129S-Gt(ROSA)26Sortm35.1(CAG-aop3/GFP)Hze/J
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Ai35D (or Ai35Δneo) mice heterozygous for the Rosa-CAG-LSL-Arch-GFP-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream Arch-GFP fusion gene (see below for detailed description of Arch-GFP). Because the CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, Arch-GFP expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the Arch-GFP fusion protein. The donating investigator reports that Ai35D mice do not express Arch-GFP prior to introduction of Cre recombinase. Fusion protein expression following exposure to cre can be detected by GFP fluorescence and mRNA (in situ hybridization) [and presumably by antibody staining (immunohistochemistry); although this was not test .....
For more information please see the full phenotype on the strain data sheet
014538 B6;129S-Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J
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Ai38 mice heterozygous for the Rosa-CAG-LSL-GCaMP3-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream GCaMP3 fusion gene (see below for detailed description of GCaMP3). Because the CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, GCaMP3 expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the fluorescent calcium indicator protein, GCaMP3. The donating investigator reports that Ai38 mice do not express GCaMP3 prior to introduction of Cre recombinase. Following exposure to Cre recombinase, GCaMP3 expression (EGFP fluorescence) is detected in the cre-expressing tissues. In the absence of calcium binding, low EGFP fluorescence is reported. Following calcium binding (s .....
For more information please see the full phenotype on the strain data sheet
014539 B6;129S-Gt(ROSA)26Sortm39(CAG-hop/EYFP)Hze/J
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Ai39 mice heterozygous for the Rosa-CAG-LSL-eNpHR3.0-EYFP-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream eNpHR3.0-EYFP fusion gene (see below for detailed description of eNpHR3.0-EYFP). Because the CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, eNpHR3.0-EYFP expression is determined by which tissue(s) express Cre recombinase. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissues; resulting in expression of the eNpHR3.0-EYFP fusion protein. The donating investigator reports that Ai39 mice do not express eNpHR3.0-EYFP prior to introduction of Cre recombinase. Fusion protein expression following exposure to cre can be detected by EYFP fluorescence and mRNA (in situ hybridization) [and presumably by antibody staining (immunohistochemistry); although this was .....
For more information please see the full phenotype on the strain data sheet
021875 B6;129S-Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze/J
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Ai65(RCFL-tdT)Δneo mice (also called Ai65(RCFL-tdT)D, Ai65Δneo, or Ai65D) harbor the Rosa-CAG-FrtSTOPFrt-LoxSTOPLox-tdTomato::deltaNeo conditional allele designed with both an frt-flanked STOP cassette and a loxP-flanked STOP cassette preventing transcription of the tdTomato fluorescent protein. Although under control of the endogenous Gt(ROSA)26Sor promoter/enhancer regions and the CAG hybrid promoter, widespread expression of tdTomato is prevented by both STOP cassettes. After removal of both flanked STOP cassettes via Flp- and cre-mediated recombination, expression of tdTomato is expected in cells/tissues where the expression patterns of the individual promoters driving FLP recombinase and Cre recombinase overlap.

Specifically, the donating investigator reports that Ai65(RCFL-tdT)Δneo mice have no tdTomato expression prior to introduction of both FLP and Cre recombinase. tdTomato fluorescence is observed in cells expressing .....
For more information please see the full phenotype on the strain data sheet

021876 B6;129S-Gt(ROSA)26Sortm66.1(CAG-tdTomato)Hze/J
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Ai66(RCRL-tdT)Δneo mice (also called Ai66(RCRL-tdT)D, Ai66Δneo, or Ai66D) harbor the Rosa-CAG-RoxSTOPRox-LoxSTOPLox-tdTomato::deltaNeo conditional allele designed with both a Rox-flanked STOP cassette and a loxP-flanked STOP cassette preventing transcription of the tdTomato fluorescent protein. Although under control of the endogenous Gt(ROSA)26Sor promoter/enhancer regions and the CAG hybrid promoter, widespread expression of tdTomato is prevented by both STOP cassettes. After removal of both flanked STOP cassettes via dre- and cre-mediated recombination, expression of tdTomato is expected in cells/tissues where the expression patterns of the individual promoters driving Dre recombinase and Cre recombinase overlap.

Specifically, the donating investigator reports that Ai66(RCRL-tdT)Δneo mice have tdTomato fluorescence in cells expressing both Dre and Cre recombinase (via breeding to Dre and Cre recombinase-expressing mice or .....
For more information please see the full phenotype on the strain data sheet

010618 B6;129S-Jag1tm2Grid/J
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These mice possess loxP sites on either side of exon 4, which encodes the DSL (Delta-Serrate-Lag2) domain, of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in the cre-expressing tissue(s). The exon 4-deleted allele produces a nonfunctional JAG1 protein.

When bred to a strain with Cre recombinase expression during development in the telencephalon and discrete head structures, such as the otocyst (see Stock No. 006084 for example), this mutant mouse strain may be useful in studies of developmental inner ear defects.

When bred to a strain with Cre recombinase expression in endothelial cells during embryogenesis and adulthood (see Stock No. For more information please see the full phenotype on the strain data sheet

024177 B6;129S-Kdm6atm1.1Kaig/J
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The Kdm6a gene encodes a histone demethylase that catalyzes the demethylation of tri/dimethylated lysine 27 of histone H3. These mice possess loxP sites on either side of exon 24 of the targeted gene. Homozygous females and hemizygous males (this gene is X linked) are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 24 deleted in the cre-expressing tissues.

When female mutant mice are bred to a strain with widespread Cre recombinase expression (see Stock No. 003724), or to a strain with Cre recombinase in spermatids (see Stock No. 003328), this mutant mouse strain may be useful in studies of development.

017692 B6;129S-Ppp3r1tm2Grc/J
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LoxP sites flank exons 3-5 of the Calcineurin β1 (Ppp3r1, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I)) gene in these floxed mutant mice. When bred to Cre-recombinase mice, excision of the floxed exons can be directed in a tissue/cell-specific manner in the resulting offspring. Disruption of the calcineurin β1 subunit results in a lack of calcineurin enzymatic activity in non-germline cell types. Ppp3r1 is a Ca2+-binding regulatory subunit of heterodimeric calcineurin, a Ca2+- and calmodulin-dependent serine/threonine protein phosphatase involved in a number of cellular processes and calcium-dependent signaling pathways.

When crossed with Lck-cre mice to specifically block calcineurin expression in thymocytes, it was shown that calcineurin β1 is essential for positive but not negative selection during thymocyte development.

When bred to a strain expressing Cre recombinase in .....
For more information please see the full phenotype on the strain data sheet

017573 B6;129S-Tet2tm1.1Iaai/J
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Like TET1 and TET3, TET2 modifies DNA by hydroxylating 5 methylcytosine. A significant percentage of patients with myeloid malignancies exhibit mutations in TET2, of these, 41.5% of mutations occur in the first coding exon (mouse exon 3). These Tet2f/f mice possess loxP sites flanking exon 3. Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in generating conditional mutations to study hematopoietic stem cell self-renewal and myeloid transformation.

For example, when crossed to a strain expressing interferon-inducible Cre recombinase (see Stock No. 003556), mice exhibit increased hematopoietic stem cell self-renewal.

022394 B6;129S-Ucp2tm2.1Lowl/J
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Ucp2, uncoupling protein 2 (mitochondrial, proton carrier), encodes an inner mitochondrial membrane protein that reduces ATP production; has a role in thermogenesis, obesity and diabetes; and is a marker of mitohormesis, conferring protection to oxidative stress. These mice possess loxP sites on either side of exons 3 and 4 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3 and 4 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in MCH-expressing neurons (see Stock No. 014099 for example), this mutant mouse strain may be useful in studies of regulation of glucose homeostasis.

022727 B6;129S1-Eedtm1Sho/J
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These EedF/F mice possess loxP sites flanking exons 3-6 of the embryonic ectoderm development (Eed) gene. EED is part of polycomb repressive complexes (PRC) 2. PRC2 is involved in transcriptional repression by the addition of three methyl groups to histone 3 during chromatin condensation. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3-6 deleted in cre-expressing tissues.

For example, when crossed to mice expressing Cre recombinase in erythrocytes, Eed deficient offspring contain reduced numbers of erythrocytes reaching the R3 stage of differentiation.

016878 B6;129S4-Bmp4tm1Jfm/J
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Mice homozygous for this Bmp4floxneo allele have loxP sites flanking exon 4 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the endogenous mature peptide deleted in the cre-expressing tissues resulting in a null allele. Mice homozygous for the Bmp4floxneo allele are viable and fertile. These mutant mice may be useful in generating conditional mutations for studying the role of Bmp4 in bone morphogenetic protein signaling pathways, bone biology, cardiovascular biology, and cancer (Bmp4 misregulation is associated with cancer cell motility/invasiveness and epithelial-to-mesenchymal transition/transformation [EMT]).

For example, when crossed to a strain expressing Cre recombinase in early limb bud mesenchyme and in a subset of craniofacial mesenchyme (see Stock No. 005584), this mutant mouse .....
For more information please see the full phenotype on the strain data sheet

022837 B6;129S4-Dab2tm1Cpr/J
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The Dab2 (disabled 2, mitogen-responsive phosphoprotein) gene encodes for a protein that is a putative tumor suppressor and that has roles in clathrin-mediated endocytosis, TGF-beta receptor signaling, Treg cell function, cellular differentiation and embryonic development. These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in CD4+ T cells, this mutant mouse strain may be useful in studies of Treg cell regulation and the FOXP3 signaling pathway.

When crossed to B6.129S4-Meox2tm1(cre)Sor/J (see Stock No. 003375) Cre recombinase expression in epiblast-derived tissues results in offspring that exhi .....
For more information please see the full phenotype on the strain data sheet

021926 B6;129S4-Kdm6atm1c(EUCOMM)Jae/J
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The Kdm6a (lysine (K)-specific demethylase 6A) gene encodes a histone demethylase that acts on lysine 27 of histone H3, and is involved in regulation of embryogenesis via the WNT signaling pathway. These mice possess loxP sites on either side of exon 3 of the targeted gene. Homozygous females and hemizygous males are viable and fertile (the gene is X linked). When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.
006414 B6;129S4-Mc4rtm1Lowl/J
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The mice have a loxp-flanked transcriptional blocking (loxTB) sequence that prevents normal endogenous gene transcription and translation from the endogenous locus. As such, homozygous mice are devoid of functional mRNA in all tested regions of the brain. Homozygous mice exhibit severe early-onset obesity, accompanied by hyperphagia, increased snout-anus length and hyperinsulinemia. The function of this disrupted allele can be restored by the enzymatic activity of Cre-recombinase. These mutant mice may be useful in studies of neurobiology, obesity, diabetes, hunger/appetite, and fat and energy metabolism.

When bred to a strain expressing Cre recombinase in the hypothalamus see Stock No. 006395 for example), this mutant mouse strain exhibits as intermediate phenotype in comparison to homozygous null mice.

018354 B6;129S4-Ror2tm1.1Meg/J
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These Ror2f/f mutant mice possess loxP sites flanking exons 3-4 of the receptor tyrosine kinase-like orphan receptor 2 (Ror2) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. ROR2 is a cell surface tyrosine-protein kinase receptor expressed during development of the facial primordia, limb mesenchyme, neural crest-derived tissues, and the genital tubercle. ROR2 is involved in cartilage and bone formation, and growth plate development, and is required for Wnt5a-mediated signaling. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 3-4 deleted in the cre-expressing tissues.

For example, when bred to Tg(EIIa-cre)C5379Lmgd transgenic mice, ROR2 null mice exhibit facial malformations, and truncation of the limbs and posterior region of the embryo. ROR2 null mice die soon after birth. This .....
For more information please see the full phenotype on the strain data sheet

010944 B6;129S4-Socs3tm1Ayos/J
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Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in generating conditional mutations to study cytokine signaling, inflammatory responses, energy homeostasis and diabetes.

For example, when crossed to a strain expressing Cre recombinase in the myeloid cell lineage (see Stock No. 004781), this mutant mouse strain may be useful in studies of cytokine signalling and inflammation.

When crossed to a strain expressing Cre recombinase in the central and peripheral nervous system (see Stock No. 003771), this mutant mouse strain may be useful in studies of leptin sensitivity and obesity.

When crossed to a strain expressing Cre recombinase .....
For more information please see the full phenotype on the strain data sheet

023035 B6;129S6-Gt(ROSA)26Sortm1(CAG-tdTomato*,-EGFP*)Ees/J
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The ROSAnT-nG allele consists of a CMV enhancer/chicken beta-actin core promoter, a loxP-flanked nT cassette, and a nG cassette, all inserted into the Gt(ROSA)26Sor locus. Prior to exposure to Cre recombinase, strong red fluorescence is observed in the nuclei of cells/tissues in a widespread fashion. No EGFP expression is reported prior to cre-mediated recombination. Superficially, ROSAnT-nG mice may be distinguished from wildtype by tail or whole body epifluorescence (tdTomato fluorescence). The red fluorescence occurs in bands around the tail (reflecting the distribution of the nuclei). When bred to Cre recombinase expressing mice, offspring will have the floxed nT cassette deleted in the cre expressing cells/tissues; this allows expression of the downstream nG cassette and results in strong green fluorescence in the nuclei of cre expressing cells and future cell lineages derived from these cells. This double-fluorescent s .....
For more information please see the full phenotype on the strain data sheet
007908 B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J
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Ai14 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE::deltaNeo conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. Ai14 mice are not expected to express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre is expected to be detectable by fluorescence and mRNA (in situ hybridization). These Ai14 mice are useful as a Cre reporter strain; expressing the red fluorescent protein variant, tdTomato, following Cre-mediated recombination.

The Allen I .....
For more information please see the full phenotype on the strain data sheet

007905 B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
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Ai9 mice hemizygous for this Rosa-CAG-LSL-tdTomato-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream red fluorescent protein variant (tdTomato). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of tdTomato. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, tdTomato expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai9 mice do not express tdTomato prior to introduction of Cre recombinase and tdTomato expression following exposure to cre can be detected by fluorescence and mRNA (in situ hybridization). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-mediated recombination if .....
For more information please see the full phenotype on the strain data sheet
021938 B6;129S6-Trp63tm1Elrf/J
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Trp63 (transformation related protein 63) encodes multiple isoforms, which have roles in longevity, ageing, obesity, metabolism, and regulation of epidermal development and differentiation. These mice possess loxP sites on either side of exon 2 of the targeted Trp63 gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in cells of the epidermal basal layer, the resulting mice prematurely age, and exhibit a phenotype including blisters, skin ulceration, and senescence of hair follicle associated dermal/epidermal cells.

Germline cre mediated deletion of TRP63 isoforms results in knock out mice that also are tumor prone and develop metastatic lesions.

012604 B6;129S7-Lrp1tm2Her/J
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These LRPflox/flox mutant mice possess a floxed Neo cassette and a single loxP sites downstream of exon 2 of the targeted low-density lipoprotein (LDL) receptor-related protein (LRP) 1 gene, Lrp1. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exons 1 and 2 deleted in the cre-expressing tissue, resulting in inactivation of Lrp1 gene function. This strain may be useful for studying physiological role of LRP in the uptake of cholesterol-rich lipoproteins from circulation and in maintenance of plasma lipid homeostasis.
007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J
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These Thy1-Brainbow 1.0 (line L) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Prior to Cre-mediated recombination, the fluorescent protein immediately adjacent to the promoter, dTomato (RFP), is expressed in peripheral and central neurons. When bred to Cre recombinase expressing mice, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): dTomato (RFP) (no recombination), mCerulean (CFP), or mYFP. Integration of tandem transgene copies yields combinatorial fluorescent protein expression in each cell, and thus many possible cell colors, providing a way to distinguish adjacent neurons and visualize other cellular interactions. Of note, the singl .....
For more information please see the full phenotype on the strain data sheet
018388 B6;SJL-Agttm1.1Itl/J
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These Agtfl mutant mice possess loxP sites flanking exon 2 of the angiotensinogen (Agt) gene. AGT is a circulating α-2-globulin produced mainly by the liver. It is the only known precursor to the peptide angiotensin II (ANG II), which is a hormone linked to hypertension. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

For example when bred to B6.Cg-Tg(Fabp4-cre)1Rev/J mice (Stock No. 005069), AGT depletion in adipocytes results in decreased systolic blood pressure and reduced circulating AGT concentrations.

012944 B6;SJL-Il6ratm1.1Drew/J
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These Il6rafl/fl mutant mice possess loxP sites flanking exons 4-6 of the interleukin 6 receptor alpha chain (Il6ra) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express tissue-specific Cre recombinase, resulting offspring lack detectable Il6ra in the cre-expressing tissues. This strain may be useful for studying the role of Il6 in immune response, re-epithelialization, angiogenesis, macrophage infiltration, and wound healing.

For example, when bred to a strain expressing Cre recombinase in the myeloid cell lineage (see Stock No. 004781 for example), this mutant mouse strain may be useful in studies of inflammation and wound healing.

When bred to a strain expressing Cre recombinase in liver (see Stock No. For more information please see the full phenotype on the strain data sheet

019012 B6N.129-Gria1tm2Rsp/J
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The GluA1fl floxed allele (also called GluR1flox, GluR-A2lox, or AQ-2lox allele) has loxP sites flanking exon 11. Homozygous mice are viable and fertile. When bred to mice that express Cre recombinase, the resulting offspring will have the glutamate receptor transmembrane domain ion channel pore deleted in cre-expressing tissues. These GluA1fl mice may be useful in generating tissue-specific AMPA-type glutamate receptor deletions. Specific examples are described below.

When GluA1fl mice are bred to a strain expressing Cre recombinase in germ-line or embryonic tissues, the resulting mice are useful in studying the pan deletion of glutamate receptor function. GluA1 knockout mice are also distributed from The Jackson Laboratory Repository as Stock No. 019011.

When GluA1fl mice are bred to a strain with Cre recombinase in parvalbumin-expressing .....
For more information please see the full phenotype on the strain data sheet

017970 B6N.129-Nlrp3tm2Hhf/J
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Nlrp3L351PneoR mice contain a floxed neomycin cassette in intron 2 of the NLR family, pyrin domain containing 3 gene, Nlrp3, in opposite orientation to gene (neoR), abolishing gene expression. These mice also contain a point mutation in exon 3, which results in a missense mutation, L351P, corresponding to human amino acid 353. This mutation is commonly found in humans with familial cold autoinflammatory syndrome (FCAS). NLRP3 encodes the protein cryopyrin, which is a cytosolic nucleotide-binding domain and leucine-rich repeat containing (NLR) protein expressed in white blood cells and chondrocytes. Cryopyrin controls the formation of the inflammasome which regulates the immune system's response to injury, toxins, and infection by cleaving interleukin (IL)-1β. NLRP3 mutations are known to cause autoinflammatory diseases known as cryopyrin-associated periodic syndromes (CAPS) such as FCAS, Muckle-Wells syndrome (MWS), and neonatal onset multisystem infl .....
For more information please see the full phenotype on the strain data sheet
017971 B6N.129-Nlrp3tm3Hhf/J
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Nlrp3D301NneoR mice contain a floxed neomycin cassette in intron 2 of the NLR family, pyrin domain containing 3 gene, Nlrp3, in opposite orientation to gene (neoR), abolishing gene expression. These mice also contain a point mutation in exon 3, which results in a missense mutation, D301N corresponding to human amino acid 303. This mutation is commonly found in humans with neonatal onset multisystem inflammatory disease (NOMID) which is characterized by systemic inflammation and skeletal abnormalities. NLRP3 encodes the protein cryopyrin, which is a cytosolic nucleotide-binding domain and leucine-rich repeat containing (NLR) protein expressed in white blood cells and chondrocytes. Cryopyrin controls the formation of the inflammasome which regulates the immune system's response to injury, toxins, and infection by cleaving interleukin (IL)-1β. NLRP3 mutations are known to cause autoinflammatory diseases known as cryopyrin-associated periodic syndromes (CA .....
For more information please see the full phenotype on the strain data sheet
011029 B6N.129-Rpl22tm1.1Psam/J
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Mice homozygous for this RiboTag allele are viable and fertile, with a targeted mutation of the ribosomal protein L22 (Rpl22) locus harboring a loxP-flanked wildtype C-terminal exon 4 followed by an identical C-terminal exon 4 that is tagged with three copies of the hemagglutinin (HA) epitope before the stop codon. Prior to exposure to Cre recombinase, RiboTag mice express the wildtype RPL22 protein (15 kDa). When the RiboTag mice are bred to cre-expressing mice, offspring will have the floxed wildtype exon 4 deleted in the cre-expressing tissue and subsequent use of the downstream HA epitope-tagged exon 4 as the terminal exon. The 23 kDa HA epitope-tagged ribosomal protein (RPL22HA) is incorporated into actively translating polyribosomes. These RiboTag mice allow Cre-mediated HA epitope tagging of ribosomes from user-defined cell types and/or immunoprecipitation of ribosomes bound to mRNA from those specific cell types.
022624 B6N.129S2(Cg)-Pik3r4tm1.1Mpnd/J
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These Vps15f/f mutant mice possess loxP sites flanking exon 2 of the phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 4, p150 (Pik3r4) gene. Vps15 (Vacuolar Protein Sorting 15) is involved in endosomal sorting and autophagy, which is required for tissue homeostasis and renewal. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

For example, when bred to B6.Cg-Tg(ACTA1-cre)79Jme/J (see Stock No. 006149) expressing Cre recombinase in skeletal muscle, resulting offspring display muscle weakness, the accumulation of autophagosomes, and glycogen and sarcolemmal features within the muscle fibers characteristic of autophagic vacuolar myopathies (AVM).

012562 B6N.Cg-Dgcr8tm1.1Blel/Mmjax
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Mice homozygous for this Dgcr82lox (or Dgcr8flox) conditional allele are viable and fertile, with loxP sites flanking exon 3 of the Dgcr8 (DiGeorge syndrome critical region gene 8) locus. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed region deleted in cre-expressing tissues. Such a deletion of exon 3 generates a frameshift mutation with multiple downstream premature stop codons resulting in a truncated protein missing the WW protein-protein interaction and RNA-binding domains. This deletion will disrupt Drosha/Dgcr8-dependent cleavage of precursor miRNAs in the nucleus and thus inhibit canonical microRNA biogenesis.
017907 B6N.Cg-Tg(Prnp-TARDBP)96Dwc/J
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Prp-TDP-43Wild-Type transgenic mice express a myc-tagged, human wildtype TAR DNA binding protein (huTDP-43) cDNA sequence under the direction of the mouse prion protein promoter (PrP). As expected for the PrP promoter, transgene expression is confined primarily to central nervous system (brain and spinal cord), with very low to no expression in other tested tissues (testis not examined). Anti-myc antibody staining shows huTDP-43 accumulation in the nuclei of neurons as well as glial cells of the spinal cord and brain, with a corresponding downregulation of endogenous mouse TDP-43. The transgene is flanked by loxP sites, allowing it to be removed by introduction of Cre recombinase if desired. The phenotype below describes Prp-TDP-43Wild-Type transgenic mice from founder line 96.
The Prp-TDP-43Wild-Type mice have moderate overexpression levels in total TDP-43 mRNA/protein, with an ~1.5-fold increase in total TDP-43 expression (huTDP-43-WT levels .....
For more information please see the full phenotype on the strain data sheet
017933 B6N.Cg-Tg(Prnp-TARDBP*Q331K)103Dwc/J
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Prp-TDP-43Q331K transgenic mice express a myc-tagged, human TAR DNA binding protein cDNA sequence modified to have the ALS-linked Q331K mutation (huTDP-43*Q331K), all under the direction of the mouse prion protein promoter (PrP). As expected for the PrP promoter, transgene expression is confined primarily to central nervous system (brain and spinal cord), with very low to no expression in other tested tissues (testis not examined). Anti-myc antibody staining shows huTDP-43*Q331K accumulation in the nuclei of neurons as well as glial cells of the spinal cord and brain, with a corresponding downregulation of endogenous mouse TDP-43. The transgene is flanked by loxP sites, allowing it to be removed by introduction of Cre recombinase if desired. The phenotype below describes Prp-TDP-43Q331K transgenic mice from founder line 103 (Prp-TDP-43Q331K [~1.5x]).
The Prp-TDP-43Q331K [~1.5x] mice have moderate overexpression levels in total TDP-4 .....
For more information please see the full phenotype on the strain data sheet
017930 B6N.Cg-Tg(Prnp-TARDBP*Q331K)109Dwc/J
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Prp-TDP-43Q331K transgenic mice express a myc-tagged, human TAR DNA binding protein cDNA sequence modified to have the ALS-linked Q331K mutation (huTDP-43*Q331K), all under the direction of the mouse prion protein promoter (PrP). As expected for the PrP promoter, transgene expression is confined primarily to central nervous system (brain and spinal cord), with very low to no expression in other tested tissues (testis not examined). Anti-myc antibody staining shows huTDP-43*Q331K accumulation in the nuclei of neurons as well as glial cells of the spinal cord and brain, with a corresponding downregulation of endogenous mouse TDP-43. The transgene is flanked by loxP sites, allowing it to be removed by introduction of Cre recombinase if desired. The phenotype below describes Prp-TDP-43Q331K transgenic mice from founder line 109 (Prp-TDP-43Q331K-low).
The Prp-TDP-43Q331K-low mice have approximately the same total TDP-43 mRNA/protein expr .....
For more information please see the full phenotype on the strain data sheet
023310 B6N;129-Ilktm1Star/J
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These ILKfl/fl mice possess loxP sites flanking exons 5-12 of the integrin linked kinase (Ilk) gene. ILK is a transmembrane serine/threonine protein kinase involved in gene expression, cell proliferation, migration, adhesion, and survival. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 5-12 deleted in cre-expressing tissues.

For example, when crossed to B6.Cg-Tg(ACTA1-cre)79Jme/J mice (Stock No. 006149), expressing Cre recombinase in striated muscle, this mutant mouse strain develops widespread progressive muscular dystrophy.

When crossed to STOCK Tg(KRT14-cre)1Amc/J mice (Stock No. 004782), expressing Cre recombinase in skin, this mutant mouse strain dies perinatally and exhibits skin blistering and .....
For more information please see the full phenotype on the strain data sheet

009045 C57BL/6-Apctm1Tyj/J
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Mice that are homozygous for the conditional allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The 15 coding exons are flanked by loxP sites. Germline heterozygous deletion of the floxed region results in a tumor-prone mouse similar to ApcMin animals (see Stock No. 002020). Homozygous germline deletion mice are not viable. This mutant mouse strain is useful for studies of this tumor suppressor gene and serves as a mouse model of colon cancer.
008817 C57BL/6-Arg1tm1Pmu/J
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These mice possess loxP sites on either side of exons 7 and 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 7 and 8 deleted in the cre-expressing tissue(s).

When bred to a strain with inducible Cre recombinase expression in the myeloid cell lineages (see Stock No. 004781, for example) or endothelial cells (see Stock No. 004128 , for example), this mutant mouse strain may be useful in studies of immune response to bacterial and parasitic infections.

007900 C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J
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Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.

For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation.

When crossed to a strain expressing Cre recombinase in oocytes (see Stock No. 011062), this mutant mouse strain ma .....
For more information please see the full phenotype on the strain data sheet

008517 C57BL/6-Gt(ROSA)26Sortm3(CAG-MIR17-92,-EGFP)Rsky/J
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Mice homozygous for the "miR-17-92 transgene" conditional allele are viable and fertile, with a loxP-flanked Neo-STOP cassette preventing transcription of the downstream bicistronic sequences (human miR-17-92 cluster (encoding the precursor of seven miRNA molecules; miR-17-5p, miR-17-3p, miR-18a, miR-19a, miR-20a, miR-19b and miR-92) and EGFP). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of the human miR-17-92 cluster. Because the synthetic CAG promoter driven miR-17-92 transgene was targeted for insertion into the Gt(ROSA)26Sor locus, expression of the transgene is determined by which tissue(s) express Cre recombinase. EGFP fluorescence, however, is not reported following exposure to Cre recombinase (presumably due to RNaseIII excision of the stem-loop structures encoding individual miRNA destabilizing the EGFP portion of the primary transcript .....
For more information please see the full phenotype on the strain data sheet
012343 C57BL/6-Gt(ROSA)26Sortm7(Pik3ca*,EGFP)Rsky/J
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Mice homozygous for the R26StopFLP110* conditional allele (also called P110*-transgene) are viable and fertile, with a loxP-flanked Neo-STOP cassette preventing transcription of the downstream bicistronic sequences (P110* [a constitutively active form of the mouse catalytic P110α subunit of phosphatidylinositol 3-kinase] and EGFP). When bred to mice that express Cre recombinase, offspring will have the STOP cassette deleted and subsequent expression of the P110* signal molecule and EGFP fluorescence in the cre-expressing cells. Expression of P110* leads to constitutively active PIK3 heterodimer activity; resulting in the generation of downstream effectors that mediate signal transduction cascades that control cell survival and cell cycle progression (growth, and proliferation). Of note, breeding these mice to an FLP-expressing strain will result in removal of the frt-flanked IRES-EGFP cassette.
022365 C57BL/6-Il15ratm2.1Ama/J
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These Il15rafl mice possess loxP sites flanking exons 2-3 of the interleukin 15 receptor, alpha chain (Il15ra) gene. IL-15Rα is presents mainly on dendritic cells, monocytes and macrophages, and plays a role in the induction of memory T cell proliferation, and Natural Killer (NK) cell survival and cell activation. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 2-3 deleted in the cre-expressing tissues.
017311 C57BL/6-Irf5tm1Ppr/J
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These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in dendritic cells and B cells, this mutant mouse strain may be useful in studies of innate immunity and autoimmunity.

008309 C57BL/6-Rag2tm1Cgn/J
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Mice homozygous for the RAG-2fl allele are viable and fertile, with loxP sites flanking exon 3 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for the entire RAG-2 protein) deleted in the cre-expressing tissue(s). These RAG-2fl mice may be useful in generating conditional mutations for studying the role of RAG-2 in B and T cell development (including cancer and toxicology research as a xenograft/transplant host), T and B cell receptor (V(D)J) recombination, hematopoiesis, hematology, immunology, and inflammation research.

For example, when bred to a strain with inducible Cre recombinase expression in liver and lymphocytes (see Stock No. 003556), this mutant mouse strain may be useful in studies of B and T cell development.

019106 C57BL/6N-Gbatm1.1Mjff/J
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Mutations in human GBA (glucosidase, beta, acid) can cause Gaucher disease, an autosomal recessive lysosomal (lipid) storage disease, and have been associated with late onset Parkinson's disease and Lewy body dementia. These mice express the mutant D427V GBA protein, which corresponds to the D409V mutation in the mature GBA protein, and possess loxP sites flanking exons 6 through 8. Mice that are heterozygous for the targeted mutation are viable and fertile. The Donating Investigator has not attempted to make the strain homozygous. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 6 through 8 deleted in the cre-expressing tissues and will no longer express the mutant D427V protein.
023804 C57BL/6N-Nr6a1tm1a(EUCOMM)Wtsi/J
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Nr6a1 (nuclear receptor subfamily 6, group A, member 1; also called Gcnf) is expressed in fetal ovarian cells, peaking at embryonic day 14.5 (E14.5). Both somatic stem cells and embryonic stem cells require NR6A1 to silence the activities of Pou5f1 (POU domain, class 5, transcription factor 1; also called Oct4) and Nanog (Nanog homeobox) in the transition from pluripotent to differentiated cell state. Interestingly, the gene is not required for this process in fetal ovarian germ cells and it is not required for initiation of meiosis and oogenesis.

This "Gcnf" allele carries an FRT-flanked IRES-lacZ reporter (expression unconfirmed) and neomycin resistance cassette. In addition, exon 7 of the Nr6a1 gene, encoding the ligand binding domain, is flanked by loxP sites. Homozygous embryos display morphological defects similar to those of Nr6a1 conditional knockout animals, including reduced size, impaired neural tube closure, and failure to turn f .....
For more information please see the full phenotype on the strain data sheet

023015 C57BL/6N-Tg(Camk2a-HBEGF)BNkza/J
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fDTR-line-B mice have a loxP-flanked alkaline phosphatase-polyadenylation signal upstream of a human diphtheria toxin receptor (DTR; heparin-binding epidermal growth factor-like growth factor (HBEGF)), both under the transcriptional control of the murine CamKIIα promoter. Transgene expression is seen at 8 weeks of age in the forebrain and partial midbrain. After 8 weeks of age in the dentate gyrus, mossy fibers, and hippocampal area CA1, with minimal expression in hippocampal area CA3. Homozygotes are viable, fertility has not been determined. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will express DTR in cre expressing tissues, making those cells susceptible to DT administration. fDTR-line-B mice have decreased DTR expression in CA3 pyramidal cells as compared to C57BL/6N-Tg(Camk2a-HBEGF)ANkza/J mice (Stock No. 023016), making them useful when .....
For more information please see the full phenotype on the strain data sheet
004597 C;129S4-Ptentm1Hwu/J
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These mice possess loxP sites flanking exon 5 of the targeted gene. Mice homozygous for the "floxed" allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.

For example, when crossed to a strain expressing Cre recombinase in astrocytes (see Stock No. 012887), this mutant mouse strain may be useful in studies of neurogenesis.

When crossed to a strain expressing Cre recombinase in the central nervous system (see Stock No. 004600), this mutant mouse strain may be useful in studies of glia differentiation and cerebellar development.

019421 FVB.129(Cg)-Myh9tm1.1Gac/Mmjax
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Myh9 (myosin, heavy chain 9, non-muscle) encodes a nonmuscle myosin IIA heavy chain involved in several functions including cytokinesis, cell motility and maintenance of cell shape. Genome wide association studies associate single nucleotide polymorphisms (SNPs) in MYH9 with chronic kidney disease in African-Americans. Myh9flox mutant mice possess loxP sites flanking exon 1. Homozygous mice are viable and fertile.

When Myh9flox mice are crossed with Pod::Cre mice the resulting offspring will have exon 1 deleted in kidney podocytes. Double mutant mice on the C57BL/6 background do not develop spontaneous proteinuria or renal insufficiency, however, when subjected to environmental stress via doxyrubicin hydrochloride (Adriamycin), Myh9 podocyte-deleted mice develop albuminuria, focal and segmental glomerulosclerosis, foot process fusion and foot process effacement. When crossed to mice with widespread Cre recomb .....
For more information please see the full phenotype on the strain data sheet

020455 FVB.129-Ets2tm3Rgo/J
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Homozygous Ets2flox mice are viable and fertile. The Ets2flox allele has loxP sites flanking exons 9-10 of the E26 avian leukemia oncogene 2 (Ets2) gene. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the C-terminal portion of the Ets2 DNA-binding domain deleted in the cre-expressing tissues. This is designed to result in an inactive Ets2 protein lacking a functional DNA-binding domain (the deleted allele is called Ets2db2).

When Ets2flox mice are bred to mice with Cre recombinase expression in embryonic/germ cells (MORE-Cre; Stock No. 003755), the resulting homozygous mice with pan deletion of the Ets2 DNA binding domain exhibit embryonic lethality similar to Ets2 knockout mice. Furthermore, such mice may be used to study mammary epithelial tumor microenvironment when bred to also harbor transgenes expr .....
For more information please see the full phenotype on the strain data sheet

009427 FVB.129S4(B6)-Gt(ROSA)26Sortm1Sor/J
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Homozygous mice are viable and fertile, with a loxP-flanked DNA STOP sequence preventing expression of the downstream lacZ gene. When crossed with a cre transgenic strain, the STOP sequence is removed and lacZ is expressed in cells/tissues where cre is expressed. These mutant mice may be used as a Cre-reporter strain; to test the tissue/cellular expression pattern of cre transgenic mice.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

005125 FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J
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These mice contain the firefly luciferase (luc) gene inserted into the Gt(ROSA)26Sor locus. Expression of the luciferase gene is blocked by a loxP-flanked STOP fragment placed between the luc sequence and the Gt(ROSA)26Sor promoter. This strain serves as a Cre reporter strain. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision is indicated by luciferase expression in Cre-expressing tissues. Mice that are heterozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
016977 FVB.129S6-Gt(ROSA)26Sortm1(Pik3ca*H1047R)Egan/J
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Pik3ca (phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; also called PI3K) encodes the p110alpha catalytic subunit of phosphatidylinositol 3-kinase. It is one of the most frequently mutated genes in human cancer. H1047R mutations in the kinase domain account for approximately 40% of breast cancer PIK3CA mutant alleles.

In this strain, an H1047R mutant form of Pik3ca was targeted to the Gt(ROSA)26Sor locus behind a floxed PGK-Neo stop cassette.

When crossed with an MMTV-Cre strain (see Stocks 003551 and 003553) to excise the floxed stop cassette, mutant Pik3a is expressed in mammary epithelium. Starting at 5 months of age, females develop mammary tumors as well as lymphoid and skin malignancies. The percentage of animals affected by tumors varied based on the particular MMTV-Cre strain used. Mammary tumors were ob .....
For more information please see the full phenotype on the strain data sheet

012429 FVB.Cg-Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/J
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Mice homozygous for the R26NZG allele are viable and fertile. The R26NZG allele contains a loxP-flanked PGK-Neo-4x polyA STOP cassette, FRT-flanked a nuclear localized β-galactosidase (nlslacZ)-3x polyA, and enhanced green fluorescent protein (EGFP)-1x polyA. Reporter gene expression from endogenous Gt(ROSA)26Sor promoter/enhancer regions is not expected, as a splice acceptor was not included in the construct. In the absence of Cre, reporter gene expression is prevented by the STOP sequence. After removal of the loxP-flanked STOP cassette via cre-mediated recombination, nlslacZ labels all cells where Cre recombinase is expressed. R26NZG can be converted to a Cre-dependent EGFP reporter, R26NG, by FLP-dependent germ line excision of the nlslacZ cassette. Germ line excision of the PGKNEO cassette by Cre converts R26NZG into a FLP-dependent EGFP reporter, R2 .....
For more information please see the full phenotype on the strain data sheet
018393 FVB/N-Tg(CAG-EGFP,TGFB1*)C8Kul/J
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These β1glo mice possess a CMV promoter driving expression of a loxP-flanked EGFP/polyA cassette upstream of a constitutively active HA tagged porcine transforming growth factor, beta 1 (Tgfb1) cDNA. TGF-β1 is a cytokine that regulates cell proliferation and differentiation, apoptosis, and extracellular matrix deposition. These mice show widespread expression of GFP and no TGF-β1 expression from the transgene. Mice that are hemizygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the floxed EGFP/polyA cassette deleted in cre-expressing tissues. TGF-β1 expression is seen only after the floxed sequence is removed.

For example, when crossed to a strain expressing mouse mammary tumor virus (MMTV) driven Cre recombinase (see Stock No. 003556), expression of TGF-β1 in th .....
For more information please see the full phenotype on the strain data sheet

008197 FVB/N-Tg(HTT*97Q)IXwy/J
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Mice hemizygous for the BACHD transgene are viable and fertile. Under the control of endogenous human htt regulatory machinery, BACHD mice have relatively high expression levels of a neuropathogenic, full-length human mutant Huntingtin (fl-mhtt) modified to harbor a loxP-flanked human mutant htt exon 1 sequence (containing 97 mixed CAA-CAG repeats encoding a continuous polyglutamine (polyQ) stretch). Prior to Cre recombinase exposure, BACHD mice exhibit progressive motor deficits, neuronal synaptic dysfunction, and selective late-onset neuropathology without somatic polyQ repeat instability in the aged brain. Moreover, BACHD mice reproduce a mhtt aggregation pattern reminiscent of that in adult-onset Huntington's disease (HD). Importantly, a relatively steady-state level of predominantly fl-mhtt and a small amount of mhtt N-terminal fragments present in both the nucleus and cytoplasm, are responsible for the onset and progression of neuropathology. Upon exposure to .....
For more information please see the full phenotype on the strain data sheet
016974 FVB;129P2-Mettm1Sst/J
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These c-metfl/fl floxed mutant mice possess loxP sites flanking exon 16 of the met proto-oncogene (Met) sequence. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. MET is a tyrosine kinase receptor, expressed in a wide variety of adult and embryonic tissues, which regulates mitogenesis, motogenesis, brain development, and dendrite and spine morphogenesis. MET, along with its ligand HGF (hepatocyte growth factor), has been implicated in diseases such as autism spectrum disorders (ASD), schizophrenia, and tumorigenesis. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 16, encoding a critical ATP-binding site, deleted in cre-expressing tissues. This deletion inactivates the intracellular tyrosine kinase domain of MET which is essential for MET function.

For example, when crossed to a strain e .....
For more information please see the full phenotype on the strain data sheet

009597 STOCK Adam17tm1.2Bbl/J
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Mice homozygous for this Taceflox allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed sequences deleted in the cre-expressing tissue producing a null allele. As the proinflammatory cytokine TNF-α and other other cell receptors are synthesized as membrane-bound precursors that need to be proteolytically released by functional TNF-α converting enzyme (TACE or Adam17 [a disintegrin and metallopeptidase domain 17]), these Taceflox mutant mice may be useful in generating conditional mutations for studying TNF-sheddase function, TNF-related autoimmune diseases.
006203 STOCK Ahrtm3.1Bra/J
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These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Hepatic protein expression of the conditional allele (before exon 2 excision) is equivalent to wildtype by Western blot analysis. This strain may be useful in generating tissue-specific mutants of the floxed allele for use in studies including teratogenesis and xenobiotic metabolism (including dioxin and PCB), Per-Arnt-Sim transcription factors, and fetal vascular development such as ductus venosus closure.

When bred to a strain expressing Cre recombinase in hepatocytes (see Stock No. 003574 for example), this mutant mouse strain may be useful in studies of toxicology.

When bred to a strain expressing Cre recombinase in endothelial cells (see Stock No. For more information please see the full phenotype on the strain data sheet

004339 STOCK Bdnftm3Jae/J
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These mice possess loxP sites on either side of the brain derived neurotrophic factor (Bdnf) coding region. Mice that are homozygous for this allele are viable and fertile.

When bred to a strain expressing Cre recombinase under the control of a tetracycline-responsive promoter element (see Stock No. 006224, 006234, 006244) and a strain expressing a tetracycline-controlled activator protein in brain tissues (see Stock No. 003763), this mutant mouse strain may be useful in studies of hippocampal-dependent learning and long-term potentiation.

When bred to a strain expressing Cre recombinase in neuronal cells (see Stock No. 003966 for example), this mutant mouse strain may be useful in stu .....
For more information please see the full phenotype on the strain data sheet

022457 STOCK Cxcl12tm1.1Sjm/J
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Cxcl2 (chemokine (C-X-C motif) ligand 12), expressed by perivascular stromal cells, endothelial cells and osteoblasts, is a chemokine required for hematopoietic stem cell (HSC) maintenance and retention in the bone marrow. It also promotes the proliferation and maintenance of B-lineage progenitors, and common lymphoid progenitors.

Exon 2 of the Cxcl2 gene is flanked by loxP sites in this allele, making it possible to conditionally inactivate the gene in cells that express Cre recombinase. Homozyous floxed animals are viable and fertile, born at predicted Mendelian ratios, and show no overt defects in their hematopoietic system. Excision of the floxed segment creates a frameshift mutation that is believed to block protein expression. Cre-mediated recombination in the hematopoietic system leads to defects in hematopoiesis, including hematopoietic stem cell and lymphoid progenitor depletion.

Homozygous null mice created through crosses with CMV-cre (see .....
For more information please see the full phenotype on the strain data sheet

019074 STOCK Dlk1tm1.1Jvs/J
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These Dlk1flox mice possess loxP sites flanking the last two exons of the delta-like 1 homolog (Dlk1) gene. Dlk1 is an epidermal growth factor (EGF)-like protein expressed from the paternal allele in skeletal muscle and cartilage. Expression is also seen in the developing pituitary gland, pancreas, lung, adrenal gland, and placenta. Since Dlk1 is paternally inherited, the donating investigator maintains these mice as heterozygotes, with the mutated allele always originating from the male parent. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the last two exons deleted in cre-expressing tissues. Because Dlk1 is imprinted and only expressed from the paternal allele, breeding Dlk1flox males with cre-expressing females is required to generate deleted offspring with the knockout phenotype.

For example, when males are crossed to B6.129S4-Myf5tm3(cre) .....
For more information please see the full phenotype on the strain data sheet

023066 STOCK Eif2ak3tm1.2Drc/J
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These PerkloxP/loxP mutant mice possess loxP sites flanking exons 3-5 of the eukaryotic translation initiation factor 2 alpha kinase 3 (Eif2ak3) gene. PERK is an endoplasmic reticulum (ER) transmembrane protein, expressed mainly in secretory and endocrine organs such as the pancreas. Mutations in PERK have been implicated in the onset of Wolcott-Rallison syndrome which is characterized by early onset of insulin-dependent diabetes, dwarfism, and skeletal dysplasias. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting double mutant offspring will have exons 3-5 deleted in cre-expressing tissues.

For example, when crossed to B6.FVB-Tg(EIIa-cre)C5379Lmgd/J mice (Stock No. 003724), resulting Perk-/- mice exhibit permanent neonatal diabetes, exocrine pancreas atrophy, growth ret .....
For more information please see the full phenotype on the strain data sheet

008872 STOCK En2tm6Alj/J
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Mice homozygous for the En2lox conditional allele are viable and fertile, with loxP sites flanking the coding region of exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed region (encoding the engrailed homeodomain) deleted in the cre-expressing tissues. These En2lox mice may be useful in generating conditional mutations for studying engrailed protein function in the developing mesencephalon, rhombomere 1, and jaw muscles, as well as the embryonic and adult cerebellum.
008407 STOCK Epas1tm1Mcs/J
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The Hif-2α 2-loxP allele, also called Hif-2&alphafl or Epas12lox, contains loxP sites flanking exon 2 of the endothelial PAS domain protein 1 locus (Epas1 or Hif-2α). Homozygous mice are are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon deleted in the cre-expressing tissue(s).

For example, when bred to a strain with inducible Cre recombinase expression in cardiac cells (see Stock No. 005657 for example), this mutant mouse strain may be useful in studies of erythropoiesis.

When bred to a strain with tamoxifen inducible widespread Cre recombinase expression(see Stock No. 008085 for example), this mutant mouse strain may be useful in studie .....
For more information please see the full phenotype on the strain data sheet

022616 STOCK Ezh2tm2Sho/J
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These Ezh2F/F mice possess loxP sites flanking exons 14-15 of the zeste homolog 2 (Ezh2) gene. EZH2 is part of polycomb repressive complexes (PRC) 2. PRC2 is involved in transcriptional repression by the addition of three methyl groups to histone 3 during chromatin condensation. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 14-15 deleted in cre-expressing tissues.

For example, when crossed to B6.Cg-Tg(Mx1-cre)1Cgn/J mice (Stock No. 003556) expressing Cre recombinase after poly I:C treatment, Ezh2 deficiency leads to the development of spontaneous T-cell leukemia (T-ALL) 122-281 days following treatment.

022620 STOCK Foxa2tm1Khk/J
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These Foxa2loxp/loxp mutant mice possess loxP sites flanking exon 3 of the forkhead box A2 (Foxa2) gene. Also known as hepatocyte nuclear factor 3 β (HNF3β), FOXA2 is a transcription factor involved in embryonic development, cellular differentiation, and gene expression during liver, pancreas and lung development. FOXA2 plays a role in glucose homeostasis and fat metabolism. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in cre-expressing tissues.

For example, when crossed to B6.Cg-Tg(Ins2-cre)25Mgn/J mice (Stock No. 003573) expressing Cre recombinase in pancreatic beta cells, double mutant mice exhibit abnormal islet and beta cell morphology and decreased glucagon secretion. They also have elevated glycogen and insulin levels. .....
For more information please see the full phenotype on the strain data sheet

021197 STOCK Gabrg2tm1Wul/J
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016830 STOCK Gabrg2tm2Lusc/J
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These mice possess loxP sites on either side of exon 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 8 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in glutamatergic neurons, this mutant mouse strain may be useful in studies of postsynaptic clustering of GABA-A receptors during synaptogenesis.

013731 STOCK Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/J
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Mice homozygous for the R26R-Confetti conditional allele are viable and fertile, with a CAG promoter, loxP site, and STOP cassette preventing transcription of the downstream Brainbow 2.1 sequences. The Brainbow 2.1 region contains two loxP-flanked dimers, each uniquely positioned in head-to-tail tandem. One dimer has nuclear-localized green fluorescent protein (hrGFPII) and a reverse-oriented cytoplasmic yellow fluorescent protein (mYFP). The other dimer has cytoplasmic red fluorescent protein (tdimer2(12)) and a reverse-oriented membrane-tethered cyan fluorescent protein (mCerulean). The Brainbow2.1 region may be written as loxP-STOP-loxP-GFP-PFY-Pxol-loxP-RFP-PFC-Pxol to show the transcriptional direction of each part. When bred to mice that express Cre recombinase, the resulting offspring may have a recombination event that stochastically places one of the four fluorescent proteins into position directly downstream of the CAG promote .....
For more information please see the full phenotype on the strain data sheet
006331 STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
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Homozygous "ROSA26-eGFP-DTA" mice are viable, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator reports that some homozygous males are subfertile. Mutant mice display widespread expression of EGFP, but DTA transcription is prevented by a strong transcriptional stop sequence. When bred to mice that express Cre recombinase under the control of a promoter of interest, the loxP-flanked EGFP and stop sequence are removed, and DTA expression is activated, resulting in the specific ablation of cre-expressing cells. This strain may be useful on its own as a fluorescent reporter or in combination with cre-expressing mice to produce conditional deletions of specific groups of cells. Transgenic mice also may have applications in toxicology and protein synthesis research.

For example, when crossed to a strain expressing Cre recombinase in the midbrain and dorsal spinal cord (see Stock No. For more information please see the full phenotype on the strain data sheet

008159 STOCK Gt(ROSA)26Sortm1(Notch1)Dam/J
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These mice contain a sequence encoding an intracellular portion of the mouse Notch1 gene (amino acids 1749-2293), but lacking the c-terminal PEST domain, and Green Fluorescent Protein, GFP, inserted into the GT(ROSA)26Sor locus. Expression of the Notch1 fragment and GFP is blocked by a loxP-flanked STOP fragment placed between the coding sequence and the GT(ROSA)26Sor promoter. The GFP expression is localized to the nucleus by an IRES sequence. The truncated cytoplasmic fragment encoded by the Notch1 sequence causes constitutive signaling activity. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants for studying the effects of Notch pathway activation. Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

For example, when crossed to a strain expressing a tamoxifen inducible Cre recombinase in all c .....
For more information please see the full phenotype on the strain data sheet

005130 STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
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These mice contain an Enhanced Yellow Fluorescent Protein/Smoothened homolog (Drosophila) fusion gene inserted into the Gt(ROSA)26Sor locus. The mutant allele consists of a fusion product involving Enhanced Yellow Fluorescent Protein (EYFP) and the constitutively active W539L point mutation of the mouse smoothened homolog (Drosophila) gene (SmoM2). Expression of the Smo/EYFP fusion gene is blocked by a loxP-flanked STOP fragment placed between the Gt(ROSA)26Sor promoter and the Smo/EYFP sequence. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision results in the constitutive expression of mouse smoothened homolog (Drosophila) and unrestrained Hedgehog signaling in Cre-expressing tissues. Expression of the SmoM2 fusion protein can be monitored using EYFP-specific fluorescence protocols. Mice that are homozygous for the mutant allele are viable, fertile, normal in size and do not display any gross physical .....
For more information please see the full phenotype on the strain data sheet
011011 STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J
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Mice homozygous for both targeted mutations (R26rtTA and Col1a12lox-4F2A) are viable and fertile. These double mutant R26rtTA;Col1a12lox-4F2A mice have widespread expression of the optimized form of reverse tetracycline-controlled transactivator (rtTA-M2) protein directed to multiple tissues by the Gt(ROSA)26Sor promoter. In the absence of the tetracycline analog doxycycline (dox), expression of the loxP-flanked, dox-inducible 4F2A cassette from the Col1a1 locus is not detected. Following dox administration, this single-gene transgenic mouse strain expresses the polycistronic 4F2A cassette (four mouse reprogramming genes Oct4 [Pou5f1], Sox2, Klf4, and c-Myc [Myc]). Somatic expression of these reprogramming factors allows multiple somatic cell types to be directly reprogrammed to generate induced pluripotent stem cells (iPSCs) by culture in dox (see details below). Because the 4F2A reprogramming .....
For more information please see the full phenotype on the strain data sheet
007576 STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
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Mice homozygous for this mT/mG mutation are viable and fertile. These mice possess loxP sites on either side of a membrane-targeted tdTomato (mT) cassette and express strong red fluorescence in all tissues and cell types examined. Tail or whole body epifluorescence is sufficient to identify mT/mG homozygotes. When bred to Cre recombinase expressing mice, the resulting offspring have the mT cassette deleted in the cre expressing tissue(s), allowing expression of the membrane-targeted EGFP (mG) cassette located just downstream. The donating investigator reports that the ACTB promoter allows stronger and persistent expression of the fluorescent proteins (especially in adult cells) compared to the endogenous Gt(ROSA) locus alone. This double-fluorescent system allows direct live visualization of both recombined and non-recombined cells at single cell resolution, offering an internal control for phenotypic analysis of Cre-induced mosaic mutants and providing a second marker fo .....
For more information please see the full phenotype on the strain data sheet
013749 STOCK Iis2tm1(ACTB-EGFP,-tdTomato)Luo/J
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Homozygous MADM-11GT mice are viable and fertile with no gross behavioral or observable abnormalities. The MADM-11GT allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP), a beta-globin intronic sequence containing an frt site and a loxP-flanked neomycin resistance gene, and the MYC-tagged C-terminal portion of a red fluorescent protein (tdTomato) all inserted into the Hipp11 locus on chromosome 11 (cytoband A1 at ~3cM between the Eif4enif1 and Drg1 loci). These MADM-11GT mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to MADM-11TG mice harboring a reciprocal mutation at the same locus (see Stock No. 013751). The resulting GT/TG offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygou .....
For more information please see the full phenotype on the strain data sheet
013751 STOCK Iis2tm2(ACTB-tdTomato,-EGFP)Luo/J
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Homozygous MADM-11TG mice are viable and fertile with no gross behavioral or observable abnormalities. The MADM-11TG allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of a red fluorescent protein (tdTomato), a beta-globin intronic sequence containing an frt site and a loxP-flanked neomycin resistance gene, and the C-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP) all inserted into the Hipp11 locus on chromosome 11 (cytoband A1 at ~3cM between the Eif4enif1 and Drg1 loci). These MADM-11TG mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to MADM-11GT mice harboring a reciprocal mutation at the same locus (see Stock No. 013749). The resulting TG/GT offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous"), and mu .....
For more information please see the full phenotype on the strain data sheet
018977 STOCK Kdrtm2Sato/J
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These VEGFR2e3loxP/e3loxP mutant mice possess loxP sites flanking a frt-flanked PGK-neo cassette and exon 3 of the kinase insert domain protein receptor (Kdr) gene. KDR, also known as VEGFR2 (vascular endothelial growth factor receptor 2), is a type III receptor tyrosine kinase which mediates VEGF-induced regulation of angiogenesis, vascular development, vascular permeability, and embryonic haematopoiesis. Homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.
017861 STOCK Kitltm2.1Sjm/J
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These mice possess loxP sites on either side of exon 1 of the Kitl (kit ligand; also called Scf, stem cell factor) gene. Mice that are homozygous for this allele are viable and fertile with no observed abnormalities. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 1 deleted in cre-expressing tissues.

Mice generated from a cross of these mice with Ubc-creER animals (e.g. Stock No. 008085) can be treated with tamoxifen to induce widespread deletion of the floxed segment. These mice become anemic and show significantly lower red blood cell counts, trend towards lower white blood cell and pletelet counts, and exhibit approximately twofold reductions in the overall cellularity of bone marrow and spleen.

018989 STOCK Leprtm1Jke/J
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These mice contain loxP flanked transcription blocker (loxTB) sequence between exons 16 and 17 of the leptin receptor (Lepr) gene, abolishing transcription of the exons downstream of the loxTB. Leptin is an adipose-derived hormone that acts to regulate food intake, energy expenditure, and body weight. LeprloxTB mice, lacking the long (B) isoform of the leptin receptor, are obese, hyperglycemic, and hyperinsulinemic, and have reduced energy expenditure. Mice that are heterozygous for the targeted mutation are viable and fertile, while homozygous mice are infertile. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have the loxTB removed and Lepr expression will be restored in cre expressing cells.

For example, when bred to STOCK Tg(Pomc1-cre)16Lowl/J mice (Stock No. 005965) expressing Cre-recombinase in proo .....
For more information please see the full phenotype on the strain data sheet

023913 STOCK Lin28atm1.2Gqda/J
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LIN28A (lin-28 homolog A (C. elegans)) is an RNA-binding protein that blocks biogenesis of Mirlet7 (let-7) microRNAs. Let-7 targets are known regulators of mammalian body size and metabolism.

Exon 2 of the gene is flanked by loxP sites in this conditional targeted mutation strain. Cre excision of the floxed segment produces a knockout allele.

Muscle-specific knockout mice created through crosses with Myf5 (myogenic factor 5)-cre animals display glucose intolerance and reduced Insr (insulin receptor) expression.

Mice created from crosses with germline-specific Ddx4-cre mice (see Stock No. 006954) show dwarfism as early as E13.5 and by E18.5 show 20% reduction in weight relative to heterozygous controls. Although born at expected Mendelian ratios, roughly 93% of Lin28a knockout mice die within 1 day of birth (2 out of 5 mice harboring a cardiac ventricular septal defect). .....
For more information please see the full phenotype on the strain data sheet

023915 STOCK Lin28atm2.1Gqda Lin28btm2.1Gqda/J
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Human LIN28A (lin-28 homolog A (C. elegans)) and LIN28B (lin-28 homolog A (C. elegans)) genes have been implicated in growth and glucose metabolism.

In this targeted mutation strain, the exon 2 sequences in both Lin28a and Lin28b have been flanked by loxP sites. Cre excision of the floxed segments creates knockout alleles. Mice homozygous for both floxed alleles are viable and fertile.

Mice homozygous for a knockout of both genes (produced by crosses with germline cre mice, e.g. Stock No. 006954) show developmental delays and embryonic lethality.

023914 STOCK Lin28btm1.2Gqda/J
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Lin28b (lin-28 homolog B (C. elegans)) is highly expressed during normal embryogenesis and is upregulated in some cancers to potently and selectively block the maturation of let-7 (Mirlet7) tumor suppressor microRNA's.

Exon 2 of the gene, encoding the functional cold shock domain, was flanked by loxP sites in this conditional mutation strain. Cre-mediated excision of the floxed segment creates a knockout allele.

Male (but not female) knockout mice created through crosses with germline-specific Ddx4-cre mice (e.g. Stock No. 006954) show postnatal dwarfism and reduced organ weights.

Skeletal muscle-directed knockouts of Lin28b, created through crosses with Myf5 (myogenic factor 5)-cre mice, produce males with phenotypic dwarfism that recapitulates that of the germline deletion. Muscle-specific loss of LIN28B leads to insulin resistance and impaired glucose uptake.

017508 STOCK Mir137tm1Mtm/Mmjax
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Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR137 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR137 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
018545 STOCK Mir34atm1.2Aven/J
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The miR-34 family of miRNAs (miR-34a, miR-34b, and miR-34c) are expressed mainly in the testis, brain, and lung. miR-34 proteins are induced upon p53 activation and are associated with tumor suppression, aging, neurodegeneration, and spermatogenesis. miR-34afl/fl mice possess loxP sites flanking the entire microRNA 34a (miR-34a) sequence. Homozygous miR-34afl/fl mice are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have miR-34a sequence deleted in the cre-expressing tissues. When crossed to mice expressing CAG-cre, miR-34a-/- mice exhibit abnormal neuronal precursor proliferation. When cre recombined miR-34a-/- mice are bred to Mirc21-/- mice (Stock No. 018546), which are deficient in miR-34b and miR-34c, the double mutant mice lack all three miR-34 family .....
For more information please see the full phenotype on the strain data sheet
008458 STOCK Mirc1tm1.1Tyj/J
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The miR-17~92 (Mir17, Mir18, Mir19a, Mir20a, Mir19b-1, Mir92-1) cluster, overexpressed in human cancers, is flanked by loxP sites in this targeted mutation strain. Mice homozygous for the floxed miR17~92 allele (miR17~92fl/fl) are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.
017513 STOCK Mirc24tm1Mtm/Mmjax
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Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR322 cluster (Mirc24) stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR322 cluster stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
017639 STOCK Nf1tm1Par/J
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Mutation in the human neurofibromin gene, NF1, is the cause of the autosomal dominant disorder Type I Neurofibromatosis. These mice possess loxP sites on either side of exons 31 and 32 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 31 and 32 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in the developing neural tube (see Stock No. 009107 for example), this mutant mouse strain may be useful in studies of Type I Neurofibromatosis.

When bred to a strain with Cre recombinase expression in neuronal cells (see Stock No. 003966 for example), this mutant mouse strain may be us .....
For more information please see the full phenotype on the strain data sheet

022363 STOCK Ntrk2tm1Ddg/J
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Ntrk2 (neurotrophic tyrosine kinase, receptor, type 2; also called TrkB) is a receptor for brain derived neurotrophic factor (Bdnf), a neurotrophin which controls aspects of mammalian nervous system development.

These mice carry a floxed F616A mutation in exon 14 of the mouse Ntrk2 gene. The allele is sensitive to a family of small-molecule inhibitors including 1NaPP1 and 1NMPP1 which act selectively, rapidly and reversibly. Without 1NaPP1 or 1NMPP1 application, no apparent phenotype is observed in the mice. In cortical neurons cultured from mutant mice, BDNF induced NTRK2 phosphorylation and its downstream signaling events are robustly and specifically blocked by nanomolar concentrations of 1NMPP1 or 1NaPP1. These compounds do not block wild-type signaling. 1NMPP1 application during pregnancy leads to a 50% reduction in the number of nodose ganglion neurons. 1NMPP1 treatment during adulthood leads to degeneration of selective motor neuron terminals.

019081 STOCK Pik3c3tm1c(EUCOMM)Wtsi/J
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These Vps34f/f mutant mice possess loxP sites flanking exon 4 of the phosphoinositide-3-kinase, class 3 (Pik3c3) gene. Also known as Vsp34, Pik3c3 generates phosphatidylinositol 3-phosphate, a regulator of membrane trafficking such as autophagy and endocytosis. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in the cre-expressing tissues.

For example, when bred to B6.Cg-Tg(Alb-cre)21Mgn/J mice (Stock No. 003574) expressing cre recombinase in liver, resulting mice develop hepatomegaly and hepatic steatosis, with impaired protein turnover. These double mutant mice exhibit decreased survival, with 50% lethality in the first 2 months of life.

For example, when bred to B6.FVB(129S4)-Tg(Ckmm-cre)5Khn/J mice (Stock No. For more information please see the full phenotype on the strain data sheet

014141 STOCK Prkaa1tm1.1Sjm/J
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These Prkaa1 mutant mice possess loxP sites flanking exon 3 of the protein kinase, AMP-activated, alpha 1 catalytic subunit (Prkaa1) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Prkaa1 belongs to the serine/threonine protein kinase family and is a catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a sensor of the energy status of cells and promotes survival during stress. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues. When bred to transgenic mice expressing Cre recombinase driven by myxovirus (influenza virus) resistance 1 (Mx1) promoter/enhanced elements, PRKAA1 expression is abolished in hematopoietic cells. This strain may be useful for studying cell growth and energy expenditure.
014142 STOCK Prkaa2tm1.1Sjm/J
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These Prkaa2 mutant mice possess loxP sites flanking exon 2 of the protein kinase, AMP-activated, alpha 2 catalytic subunit (Prkaa2) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Prkaa2 belongs to the serine/threonine protein kinase family and is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a sensor of the energy status of cells and ensures survival during stress. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues. When bred to transgenic mice expressing Cre recombinase driven by myxovirus (influenza virus) resistance 1 (Mx1) promoter/enhanced elements, PRKAA2 expression is abolished in hematopoietic cells. This strain may be useful for studying cell growth and energy expenditure, as well as bladder cancer.
022972 STOCK Pros1tm1Grl/J
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Exons 11-15 protein S (alpha) (Pros1) gene are flanked by loxP sites. The frt-flanked neo is still present in this strain, and the donating investigator reports no change in phenotype due to the neo cassette. PROS1 is a vitamin K-dependent blood anticoagulant expressed in the endothelium. It functions in the removal of apoptotic cells from circulation and degradation of factors Va and VIIIa. It has also been implicated in vascular development and homeostasis. Deficiencies have been associated with venous thrombosis, stroke, and autoimmunity. Mice that are homozygous for this floxed allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 11-15 deleted in cre-expressing tissues.

When bred to B6.FVB-Tg(EIIa-cre)C5379Lmgd/J mice (Stock No. 003724) expressing cre in the developing embryo, homozygous offspring .....
For more information please see the full phenotype on the strain data sheet

005550 STOCK Rac1tm1Djk/J
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These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.

When bred to a strain expressing Cre recombinase in the myeloid cell lineage (see Stock No. 004781 for example), this mutant mouse strain may be useful in studies of neutrophil function.

When bred to a strain expressing a tamoxifen inducible Cre recombinase specific to keratinocytes(see Stock No. 005107 for example), this mutant mouse strain may be useful in studies of stem cell renewal in the epidermis.

020649 STOCK Rictortm1.1Klg/SjmJ
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These RictorF/F mutant mice possess loxP sites flanking exon 11 of the RPTOR independent companion of MTOR, complex 2 (Rictor) gene. Mice that are homozygous for this allele are viable and fertile. Rictor is part of a complex mTORC2, which regulates cell growth and survival. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 11 deleted in the cre-expressing tissues.

For example, when bred to C.Cg-Tg(Mx1-cre)1Cgn/J mice (Stock No. 005673) expressing cre in interferon-responsive cells, Rictor deletion in thymocytes in both bone marrow and thymus, after induction with polyinosinic-polycytidylic acid (pIpC), results in reduced proliferation of immature thymocytes.

013086 STOCK Rxratm1Krc/J
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These mice possess loxP sites on either side of exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in liver (see Stock No. 003574 for example), this mutant mouse strain may be useful in studies of hepatocyte proliferation and liver regeneration.

When bred to a strain with Cre recombinase expression in myeloid cell lineages (see Stock No. 004781 for example), this mutant mouse strain may be useful in studies of glomerulonephritis, autoimmunity and systemic lupus erythematosus (SLE).

017334 STOCK Sirt6tm1.1Cxd/J
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These Sirt6Co floxed mutant mice possess loxP sites flanking exons 2-3 of the sirtuin 6 (Sirt6) targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Sirt6 is a histone deacetylase highly expressed in the central nervous system, which is involved in the regulation of glucose homeostasis, cell fate, and genomic stability. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2-3 deleted in cre-expressing tissues.

For example, when crossed to a strain expressing Nestin-Cre in the central and peripheral nervous system (see Stock No. 003771), this mutant mouse strain exhibits reduced post-natal growth and obesity.

When crossed to a strain expressing Cre Recombinase in the liver (see Stock No. For more information please see the full phenotype on the strain data sheet

022074 STOCK Smad2tm1.1Epb/J
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These Smad2f/f mice possess loxP sites flanking exon 2 of the SMAD family member 2 (Smad2) gene. SMAD2 is an intracellular signaling mediators involved in growth control and transcriptional regulation during development by transmitting chemical signals from the cell surface to the nucleus via the transforming growth factor β (TGFβ) signaling system. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in cre-expressing tissues.

For example, when crossed to B6.Cg-Tg(Cdh16-cre)91Igr/J mice (Stock No. 012237) expressing Cre recombinase in the kidney, TGF-β signaling is enhanced which promotes increased fibrosis.

When crossed to B6.Cg-Tg(Alb-cre)21Mgn/J mice (Stock No. 003574) expressing .....
For more information please see the full phenotype on the strain data sheet

017462 STOCK Smad4tm2.1Cxd/J
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These Smad4Co floxed mutant mice possess loxP sites flanking exon 8 of the MAD homolog 4 (Smad4) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. SMAD4 is a member of a family of intracellular signaling mediators and plays a role as a tumor suppressor. SMAD4 is involved in growth control and transcriptional regulation during development by transmitting signals from the cell surface to the nucleus via the transforming growth factor β (TGFβ) and bone morphogenic protein (BMP) signaling system. When these mutant mice are bred to mice expressing Cre recombinase, resulting offspring will have exon 8 deleted in cre-expressing tissues. This strain may be useful for studying cell proliferation and tumor suppression. For example, when crossed to a strain expressing Cre recombinase in the pancreas, this mutant mouse strain may be useful in studi .....
For more information please see the full phenotype on the strain data sheet
004526 STOCK Smotm2Amc/J
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These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

When bred to a strain with the targeted null allele (Stock No. 004288) and a strain expressing Cre recombinase in the skin (Stock No. 004782), this mutant mouse strain may be useful in studies of hedgehog signalling and cell proliferation in the dental epithelium.

When bred to a strain with the targeted null allele (Stock No. 004288) and a strain expressing Cre recombinase in the nervous system (Stock No. 003771), this mutant mouse strain may be useful in studies of hedgehog signalling and cerebellar foliation.

When bred to .....
For more information please see the full phenotype on the strain data sheet

013093 STOCK Sox2tm1.1Lan/J
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These Sox2flox mutant mice possess loxP sites flanking the coding exon of SRY-box containing gene 2 (Sox2). Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express tissue-specific Cre recombinase, the resulting offspring will have no Sox2 expression in cre-expressing tissues. This strain may be useful for studying eye and lens development.
023415 STOCK Sp8tm2Smb/J
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These Sp8flox mutant mice possess loxP sites flanking the coding region of the trans-acting transcription factor 8 (Sp8) gene. SP8 is a transcription factor that plays a role in embryonic limb development, craniofacial development, and olfactory neurogenesis. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have the entire coding region deleted in the cre-expressing tissues.

For example, when bred to mice having germ-line Cre expression, resulting homozygous mice exhibit defects of both forelimbs and hindlimbs, tail abnormalities, defects in anterior and posterior neuropore, exencephaly and spina bifida.

014143 STOCK Stk11tm1.1Sjm/J
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These Stk11 mutant mice possess loxP sites flanking exons 3-6 of the serine/threonine kinase 11 (Stk11) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Stk11 is a member of the serine/threonine kinase family and regulates cell polarity and cell division, and controls cell energy expenditure. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 3-6 deleted in the cre-expressing tissues. When bred to transgenic mice expressing Cre recombinase driven by myxovirus (influenza virus) resistance 1 (Mx1) promoter/enhanced elements, STK11 expression is abolished in hematopoietic cells. This strain may be useful for studying cell growth and tumor suppression.

For example, when crossed to a strain expressing tamoxifen-inducible Cre recombinase (see Stock No. For more information please see the full phenotype on the strain data sheet

017635 STOCK Stk4tm1.1Rjo Stk3tm1.1Rjo/J
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Stk4 (serine/threonine kinase 4 ) and Stk3 (serine/threonine kinase 3) are the mouse orthologs for the Drosophila hippo gene. Both Stk4 and Stk3 are key components of the Salvador-Warts-Hippo (SWH) pathway, which regulates tissue growth and organ size by restricting cell proliferation and promoting apoptosis. Mice that are homozygous for both the floxed targeted mutations are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 4-5 of the (Stk4) gene and exons 5-6 of the (Stk3) gene deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in the liver (see Stock No. 003574 for example), this mutant mouse strain may be useful in studies of hepatocyte proliferation and liver cance .....
For more information please see the full phenotype on the strain data sheet

010721 STOCK Tgfb1tm2.1Doe/J
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Mice homozygous for this Tgfb1flox-ex 6 conditional allele are viable and fertile, with loxP sites flanking exon 6 of the Tgfb1 (transforming growth factor, beta 1) gene. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed region deleted in the cre-expressing tissues while leaving the precursor pro-region and neighboring B9d2 (LOC232987) gene intact.
005680 STOCK Tsc1tm1Djk/J
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Homozygous mice are viable, fertile, normal in size, have normal expression of hamartin (the targeted gene's protein product), have no growth or behavioral defects, and are devoid of tumors through age 18 months. This mutant carries a "floxed" allele of the endogenous gene. When combined with a mutant carrying a Cre recombinase gene under the control of a promoter of interest, exons 17 and 18 of Tsc1 are deleted in the tissue of interest. This mutant may be useful in many tissue-specific studies including tuberous sclerosis or other hamartoma syndromes, regulation of the actin cytoskeleton and motility, cellular and organismal glucose homeostasis, cell growth responses, apoptosis regulation, and regulation of cell size.

When bred to a strain expressing Cre recombinase in neuronal cells (see Stock No. 003966 for example), this mutant mouse strain may be useful in studies of tuberous sclerosis.

When .....
For more information please see the full phenotype on the strain data sheet

003920 STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
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These Z/EG transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being liver and lung tissue. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with enhanced GFP expression in tissues expressing cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess cre excision activity in live animals and cells. Although homozygotes are viable, attempts to breed homozygous mice proved unsuccessful.
017919 STOCK Tg(CAG-EGFP,-dsRed2/RNAi:Tardbp)6Zxu/J
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Stock No. 017919 is the preinduction parent Tg mouse line (amiR-TDP43u line 6 or CAG-loxp-EGFP/3xpA-loxp-RFP-amiR-TDP43); a cre-inducible parental strain used to generate the neurodegenerative amiR-TDP43i transgenic mice (Stock No. 017934).

These amiR-TDP43u transgenic mice have a loxP-flanked EGFP/STOP cassette preventing transcription of a downstream red fluorescent protein (dsRed2) and an artificial miRNA that targets the mouse TAR DNA binding protein (amiR-TDP43). Prior to Cre recombinase exposure, EGFP expression is directed to widespread tissues by the CMV-IE enhancer/chicken β-actin/rabbit β-globin hybrid promoter. Mice hemizygous for the inducible transgene are viable and fertile, with no reported spino-muscular abnormalities (although the donating investigator reports very low amiR-TDP43 RNA expression prior to Cre recombinase introduction). The phenotype of homozygous mice has not been chara .....
For more information please see the full phenotype on the strain data sheet

008359 STOCK Tg(SMN2)89Ahmb Smn1tm3(SMN2/Smn1)Mrph/J
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The SMN2;SmnRes mice harbor the Tg(SMN2)89 transgene and the Smn rescue allele (SmnRes; also called Smn1 conditional inversion or Smn1 COIN). The Tg(SMN2)89Ahmb transgene consists of the entire human SMN2 (survival of motor neuron 2, centromeric) gene. The Smn rescue allele is a functional null prior to exposure to Cre recombinase (no full-length SMN transcript is detected in somatic tissue by RT-PCR). No spontaneous inversion of the allele is reported in the absence of Cre recombinase. The SmnRes allele is engineered to revert to a fully functional SMN upon exposure to Cre recombinase. Specifically, Cre recombinase will irreversibly invert the fragment bordered by the lox71 and lox66 sites, and the resulting allele is "rescued" into a format that contains mouse Smn1 exons 1-7 and human SMN2 exon 8. Because the mouse Smn1 exon 7 will be efficiently spliced, the majority of the mRNA from the Cre recombinase-induc .....
For more information please see the full phenotype on the strain data sheet
025526 129S.129P2(B6)-Ep300tm2Pkb/J
Under Development - Now Accepting Orders
Ep300 (E1A binding protein p300) is a histone acetylase that is required for normal development. It interacts with a significant percentage of mammalian transcriptional regulatory proteins, thus influences a broad range of physiological pathways.

Exon 9 of the mouse Ep300 gene is flanked by loxP sites in this conditional mutant strain. Homozygotes are viable and fertile, and appear normal. Excision of the floxed region by cre recombinase results in a knockout allele.

Knockouts created through crosses with thymocyte-specific Lck-cre mice show a decrease in the number of CD4+ CD8+ double-positive thymocytes, but an increase in the percentage of CD8+ single-positive thymocytes. Mice with high deletion frequencies are deficient for full-length and truncated protein, as measured by Western blot.

024976 B6(129S4)-Tspotm1.1Maf/J
Under Development - Now Accepting Orders
The Tspo, translocator protein, gene encodes for an outer mitochondrial membrane protein that binds to benzodiazepines and is involved in cholesterol transport and steroidogenesis. These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 and 3 deleted in the cre-expressing tissues.
024540 B6(Cg)-Gt(ROSA)26Sortm1(Sstr3/GFP)Bky/J
Under Development - Now Accepting Orders
These CiliaGFP-OFF mice carry a floxed STOP cassette upstream of a somatostatin receptor 3 protein fused with green fluorescent protein (Sstr3::GFP). Mice that are homozygous for the targeted mutation are viable and fertile. When bred to mice that express Cre recombinase, resulting offspring will have the floxed-STOP cassette deleted in the cre-expressing tissues, allowing the expression of the cilia-localized Sstr3::GFP from the ubiquitous ROSA26 promoter in those tissues.

When bred to Tg(EIIa-cre)C5379Lmgd/J transgenic mice (see Stock No. 003724) the floxed-STOP cassette is deleted, resulting in CiliaGFP-ON mice. These mice express GFP in cilia in a variety of live tissues, including brain, retina, and kidney, and after fixation. CiliaGFP-ON male mice are sterile with only a small fraction of motile spermatozoa detected. Female mice exhibit norm .....
For more information please see the full phenotype on the strain data sheet

024390 B6(Cg)-Hat1tm1Mrpa/J
Under Development - Now Accepting Orders
Hat1flox mutant mice possess loxP sites flanking exon 3 of the targeted gene. HAT1 is involved in the rapid acetylation of newly synthesized soluble cytoplasmic histones. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in cre-expressing tissues.

For example, when crossed to a strain with widespread Cre recombinase expression, HAT1 deficient mice exhibit neonatal lethality due to mesenchymal cell hyperproliferation in the lung. Mice also have craniofacial defects, including nasal cavity defects and defects or absence of the lower jaw bone. Mouse embryonic fibroblasts derived from Hat1 KO mice are sensitive to DNA damaging agents and display a high level of genome instability.

022786 B6(Cg)-Nfatc1tm3Glm/AoaJ
Under Development - Now Accepting Orders
These Nfatc1fl/fl mutant mice possess loxP sites flanking exon 3 of the nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1 (Nfatc1) gene. Mice that are homozygous for this allele are viable and fertile. NFATC1 is a transcription factor which, when upregulated by the receptor activator for nuclear factor-κB ligand (RANKL), promotes the expression of proosteoclastogenic genes and regulates osteoclast homeostasis. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in cre-expressing tissues.

For example, when crossed to B6.Cg-Tg(Mx1-cre)1Cgn/J mice (Stock No. 003556), poly I:C-induced Cre recombinase in offspring display osteopetrosis, improperly shaped long bones, failure to resorb primary spongiosa, aberrant endochondral growth and ossification.

024859 B6(Cg)-Tinf2tm2.1Tdl/J
Under Development - Now Accepting Orders
These TIN2 DC-LSL mice contain the amino acid mutation K267E in exon 6 of the Terf1 (TRF1)-interacting nuclear factor 2 (Tinf2) gene. This mutation introduces a lysine-to-glutamate mutation, analogous to mutations found in patients with dyskeratosis congenital (DC). These mice also contain a loxP-flanked Puro-4x polyA STOP cassette between exons 2 and 3. In the absence of Cre in TIN2+/DC-cond floxed mice, Tinf2 expression is prevented by the STOP sequence. After cre-mediated removal of the loxP-flanked STOP cassette, TIN2+/DC mice express TIN2 K267E DC in all cells where Cre recombinase is expressed. Hemizygous mice are viable and fertile. DC is a progressive bone marrow failure syndrome characterized by a mucocutaneous triad of oral leukoplakia, nail dystrophy, abnormal skin pigmentation, and a predisposition to cancer. TINF2 is a component of the shelterin protein complex which is required for the mainte .....
For more information please see the full phenotype on the strain data sheet
024872 B6(Cg)-Tlr4tm1.1Karp/J
Under Development - Now Accepting Orders
The Tlr4, toll-like receptor 4, gene encodes a protein that has a critical role in pathogen recognition and activation of the innate immune system. These mice possess loxP sites on either side of exon 3 of the targeted Tlr4 gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.
024632 B6(SJL)-Rpl13atm1.1Mazu/J
Under Development - Now Accepting Orders
These mice possess loxP sites on either side of exons 2 through 8 of the targeted Rpl13a gene. The protein encoded by the Rpl13a gene is a structural component of the 60S subunit of the ribosome and is also part of the gamma interferon-activated inhibitor of translation (GAIT) complex involved in inhibition of inflammatory interferon-gamma-induced transcript-selective translation. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2-8 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in the myeloid cell lineage (see Stock No. 004781 for example), this mutant mouse strain may be useful in studies of translational control in macrophages.

023727 B6.129S(FVB)-Bcl6tm1.1Dent/J
Under Development - Now Accepting Orders
These Bcl6fl/fl mutant mice possess loxP sites flanking exons 7-9 of the B cell leukemia/lymphoma 6 (Bcl6) gene. Bcl6 encodes a transcriptional repressor that regulates the differentiation of CD4 T cells into follicular Th cells (TFH). Homozygotes are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 7-9 deleted in cre-expressing tissues.

For example, when crossed to STOCK Tg(Cd4-cre)1Cwi/BfluJ mice (Stock No. 017336) expressing Cre recombinase in CD4-expressing T cells, double mutant mice exhibit almost complete loss of germinal center (GC) B cells and TFH cells when immunized with sheep red blood cells.

021443 B6.129S-Tgif1tm1Caw/J
Under Development - Now Accepting Orders
Tgif1 (TGFB-induced factor homeobox 1) is a member of the evolutionarily-conserved three-amino-acid loop extension (TALE) superclass of atypical homeodomain proteins. It acts as a transcriptional co-repressor that modulates responses to TGFB signaling and plays a role in regulating retinoic-acid-mediated gene expression. Mutation of the mouse gene results in relatively mild developmental phenotypes on most strain backgrounds. Knockout of both Tgif1 and Tgif2 results in a failure of embryonic gastrulation. Mutations in the human TGIF1 gene have been associated with holoprosencephaly.

Exons 2 and 3, encoding 98% of the amino acids in one isoform of the protein and 100% of the other, are flanked by loxP sites in this conditional knockout allele. Cre excision of the floxed segment can direct tissue-specific knockouts of the gene. Animals made homozygous for a widespread knockout via crosses with a human β-actin-driven Cre deleter line are viable and f .....
For more information please see the full phenotype on the strain data sheet

023323 B6.129S1-Cdkn2atm4Cjs/J
Under Development - Now Accepting Orders
The alternate reading frame of the Cdkn2a (cyclin-dependent kinase inhibitor-2A) locus encoding the Arf tumor suppressor, is expressed transiently during mouse male germ cell and eye development. Its inactivation compromises spermatogenesis as mice age and leads to aberrant postnatal proliferation of cells in the vitreous of the eye, resulting in blindness. The gene is frequently deleted or epigenetically silenced in a wide variety of tumors. Mice lacking Arf spontaneously develop tumors later in life. The first coding exon (exon-1β) of the mouse Arf gene is flanked by loxP sites in this conditional mutant strain. Floxed homozygotes are viable and fertile, appear phenotypically normal, and are not prone to cancer development. Cre-mediated excision of the floxed segment results in directed knockouts of the Arf gene but leave the linked Ink4a (Cdkn2a) gene intact. Infection of cultured embryonic fibroblast cells or pre-B cells with cre-expressing retroviruses ca .....
For more information please see the full phenotype on the strain data sheet
024341 B6.129S1-Reltm1Ukl/J
Under Development - Now Accepting Orders
The Rel (reticuloendotheliosis oncogene), also known as the proto-oncogene c-Rel, encodes for a member of the Rel/NF-kappaB family of transcription factors which are involved in cell growth and survival, stress responses and inflammation. These mice possess loxP sites on either side of exon 1 of the Rel gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted and GFP expression in the cre-expressing tissue(s). When crossed to mice that express Flp recombinase, the entire targeting construct, including exon 1, are deleted in the FLP expressing tissues.

When bred to a strain expressing Cre recombinase in B lymphocytes (see Stock No. 006785 for example), or to a strain expressing Cre recombinase in germinal center B lymphocytes (see Stock No. For more information please see the full phenotype on the strain data sheet

024694 B6.129S1-Tgm2tm1Rmgr/J
Under Development - Now Accepting Orders
These Tgm2t/t floxed mice possess loxP sites flanking exons 6-8 of the transglutaminase 2, C polypeptide (Tgm2) gene. The donating investigator states that the remaining frt-flanked neo cassette conveys no abnormalities. Tgm2 is a calcium-dependent enzyme that catalyzes the crosslinking of proteins. TGM2 mediates signaling by various G-protein coupled receptors and has been found to be involved in apoptosis, fibronectin stabilization, cataract development, neurodegeneration, and wound healing. TGM2 has also been cited as an autoantigen involved in celiac disease and the onset of Type 2 diabetes mellitus (T2DM). Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 6-8 deleted in the cre-expressing tissues.

For example, when bred to mice carrying the Tg(CMV-cre)1Cgn allele ( see Stock No. For more information please see the full phenotype on the strain data sheet

024496 B6.129S6(Cg)-Id3tm2.1Zhu/J
Under Development - Now Accepting Orders
These Id3f/f mutant mice possess loxP sites flanking exons 1-2 of the inhibitor of DNA binding 3 (Id3) gene. ID3 is a dominant negative helix-loop-helix (H-L-H) protein. ID3 forms nonfunctional heterodimers with other HLH proteins, E-proteins in particular, and acts to inhibit the binding of DNA to the other HLH protein. This gene is involved in the regulation of cell cycle progression and B-cell differentiation, and in mediating TCR signals during double positive (DP) to single positive (SP) selection. ID3 deficiencies have been implicated in the development of Sjogren's syndrome, a rheumatic autoimmune disease characterized by persistent dry eyes and mouth, lymphocyte infiltrates in salivary gland, impaired thymocyte selection, and serum positive for multiple autoantibodies. ID3 has also been associated with the onset of Burkitt's lymphoma, a highly aggressive B-cell non-Hodgkin lymphoma. Mice that are homozygous for this allele are viable and fertile .....
For more information please see the full phenotype on the strain data sheet
024549 B6.129S6(Cg)-Resttm1.1Jhsi/J
Under Development - Now Accepting Orders
These REST/NRSFloxP mice possess loxP sites on either side of exon 4 of the targeted gene. The Rest gene encodes for a transcriptional repressor that has an important role in neurogenesis. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in the cre-expressing tissues.

When bred to a strain with inducible Cre recombinase expression the adult and developing mouse brain (see Stock No. 016261), this mutant mouse strain may be useful in studies of adult neuroregeneration.

022361 B6.129S6(SJL)-Fgf21tm1.2Djm/J
Under Development - Now Accepting Orders
These Fgf21loxP mice possess loxP sites flanking exons 1-3 of the fibroblast growth factor 21 (Fgf21) gene. FGF21 is an endocrine hormone, expressed in liver and pancreas, which stimulates glucose uptake in adipocytes. FGF21 induction in liver by peroxisome proliferator-activated receptor a (PPARa) during fasting induces fatty acid oxidation ketogenesis in liver. Homozygotes are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 1-3 deleted in the cre-expressing tissues.

For example, when bred to B6.129S4-Meox2tm1(cre)Sor/J mice (Stock No. 003755) expressing germ line Cre-recombinase, livers from resulting offspring have decreased beta-oxidation, ketogenesis, and gluconeogenesis under fasted conditions.

023516 B6.129S7(SJL)-Glra1tm3.1Geh/J
Under Development - Now Accepting Orders
Glycine receptors are linked to the actions of alcohol and anesthetics and appear to play prominent roles in disorders such as tremor, seizures, and hyperekplexia. The contribution of G protein regulation of glycine receptor function to these processes/disorders is largely unknown.

These mice carry a conditional knock-in allele of the mouse Glra1 (glycine receptor, alpha 1 subunit) gene. When crossed with a cre strain, expression of the wildtype gene can be replaced with that of a KK385-386AA (lysine to alanine) modulatory site mutation in tissues of interest.

After crossing with a global cre deleter (EIIa-cre mice, Stock No. 003724) to completely delete the floxed wild type exon, global knock-in mice display largely normal behavior and expression as determined by Western blot. Immunocytochemistry studies also indicate that expression is similar to that of wildtype. Knock-in mice exhibit a lower sensi .....
For more information please see the full phenotype on the strain data sheet

024750 B6.Cg-Gt(ROSA)26Sortm9(EGFP/Rpl10a)Amc/J
Under Development - Now Accepting Orders
These mice contain a CAGGS (CMV-IE enhancer/chicken beta-actin/rabbit beta-globin) hybrid promoter, neomycin (neo) resistance cassette and polyA sequence, cDNA encoding an enhanced green fluorescent protein (EGFP) fused to ribosomal protein unit L10a (Rpl10a) followed by a polyA signal inserted into the Gt(ROSA)26Sor locus. Mice that are homozygous for the mutant allele are viable and fertile. Expression of the EGFP::L10A fusion gene is blocked by a loxP-flanked STOP fragment (3 copies of SV40 polyA) placed between the Gt(ROSA)26Sor promoter and the EGFP::L10A sequence. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision results in the constitutive expression of eGFP-tagged form of the L10a ribosomal subunit in Cre-expressing tissues. Activation of EGFP::L10A within a cell type allows identification of cell type-specific changes in mRNA populations at the whole-organ level by translating r .....
For more information please see the full phenotype on the strain data sheet
022519 B6.Cg-Plvaptm1.1Rvst/J
Under Development - Now Accepting Orders
The plasmalemma vesicle associated protein Plvap gene encodes an endothelial membrane glycoprotein essential for proper stomatal and fenestral endothelial diaphragm formation. These mice possess loxP sites on either side of exons 2 through 5 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have 2 through 5 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in endothelial cells (see Stock No. 004128 for example), this mutant mouse strain may be useful in studies of microvascular permeability.

When bred to mice carrying Tg(Cdh5-cre)1Spe (Stock No. 017968), Cre recombinase expression in vascular endothelial cells results in lethality (genetic background dependent), diap .....
For more information please see the full phenotype on the strain data sheet

024537 B6;129-Nlktm1.1Ecan/J
Under Development - Now Accepting Orders
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues. Exon 2 encodes for part of the kinase domain with excision of exon 2 resulting in a frameshift. The serine/threonine-protein kinase produced by the Nlk gene is related to mitogen-activated protein kinases and can regulate Wnt/beta-catenin, BMP/Smad and Notch signaling.

When bred to a strain with Cre recombinase expression in osteoblast precursor cells (see Stock No. 005657 for example), this mutant mouse strain may be useful in studies of bone homeostasis.

024105 B6;129S-Gt(ROSA)26Sortm95.1(CAG-GCaMP6f)Hze/J
Under Development - Now Accepting Orders
Ai95(RCL-GCaMP6f)-D mice (also called Ai95Δneo or Ai95D) harbor the Rosa-CAG-LSL-GCaMP6f::deltaNeo conditional allele, designed with a floxed-STOP cassette upstream of the GCaMP6 fast variant calcium indicator (GCaMP6f; see detailed description below). Although under control of the endogenous Gt(ROSA)26Sor promoter/enhancer regions and the CAG hybrid promoter, widespread expression of GCaMP6f is prevented by STOP cassettes. After exposure to Cre recombinase, bright EGFP fluorescence is observed following calcium binding (such as neuronal activation).

Specifically, the donating investigator reports Ai95D mice have no reported levels of EGFP fluorescence prior to exposure to Cre recombinase. When Ai95D mice are bred with a Cre-driver line to create double transgenic animals (GCaMP6f+/Cre+), cells expressing Cre exhibit low EGFP fluorescence in the absence of calcium binding. Following calcium binding (such as neuronal activation), greatly increased EGFP fluorescence .....
For more information please see the full phenotype on the strain data sheet

024103 B6;129S-Igs7tm93.1(tetO-GCaMP6f)Hze/J
Under Development - Now Accepting Orders
These Ai93(TITL-GCaMP6f)-D mice (also called Ai93Δhygro or Ai93D) are a Cre/Tet-dependent, fluorescent calcium indicator line utilizing an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated (TIGRE). Ai93D mice harbor the TIGRE-Ins-TRE-LSL-GCaMP6f::deltaHygro conditional allele, designed with a modified Tet response element (TRE or tetO) and loxP-flanked STOP cassette upstream of the GCaMP6 fast variant calcium indicator (GCaMP6f; see detailed description below). When bred with other mice expressing Cre recombinase, tetracycline-controlled transactivator protein (tTA) and/or reverse tetracycline-controlled transactivator protein (rtTA), GCaMP6f expression in cells/tissues where the expression patterns of the individual promoters driving Cre and tTA/rtTA overlap can be regulated with tetracycline or its analog doxycycline (dox).

Specifically, the donating investigator reports Ai93D mice have no reported levels of GC .....
For more information please see the full phenotype on the strain data sheet

024048 B6;129S-Pkmtm1.1Mgvh/J
Under Development - Now Accepting Orders
The Pkm, pyruvate kinase muscle, gene encodes different splicing variant isoenzymes which are involved in glycolysis. The pyruvate kinase M2 isoform is found in tumor cells and some normal tissues. These mice possess loxP sites on either side of exon 10 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 10 deleted in the cre-expressing tissues: pyruvate kinase M2 isoform expression is elimated, while pyruvate kinase M1 isoform expression is unchanged.
When bred to a cancer model, this mutant mouse strain may be useful in studies of acceleration of tumorigenesis and metastasis. It could also be used to test the role of PKM2 in normal tissue physiology.
024106 B6;129S6-Gt(ROSA)26Sortm96(CAG-GCaMP6s)Hze/J
Under Development - Now Accepting Orders
Ai96(RCL-GCaMP6s) mice (also called Ai96) harbor the Rosa-CAG-LSL-GCaMP6s conditional allele, designed with a floxed-STOP cassette upstream of the GCaMP6 slow variant calcium indicator (GCaMP6s; see detailed description below). Although under control of the endogenous Gt(ROSA)26Sor promoter/enhancer regions and the CAG hybrid promoter, widespread expression of GCaMP6s is prevented by STOP cassettes. After exposure to Cre recombinase, EGFP fluorescence is observed following calcium binding (such as neuronal activation).

Specifically, the donating investigator reports Ai96 mice have no reported levels of EGFP fluorescence prior to exposure to Cre recombinase. When Ai96 mice are bred with a Cre-driver line to create double transgenic animals (GCaMP6f+/Cre+), cells expressing Cre exhibit low EGFP fluorescence in the absence of calcium binding. Following calcium binding (such as neuronal activation), increased EGFP fluorescence is observed in these cells.

The donating .....
For more information please see the full phenotype on the strain data sheet

025544 B6;SJL-Nlgn2tm1.1Sud/J
Under Development - Now Accepting Orders
These FN2 floxed mice possess loxP sites flanking exons 2-5 of the neuroligin 2 (Nlgn2) gene. Mice that are homozygous for this allele are viable and fertile. NLGN2 is a transmembrane scaffolding protein involved in cell-cell interactions, and plays a role in synapse function and synaptic signal transmission. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 2-5 deleted in the cre-expressing tissues. When expression of cre in the prefrontal cortex was induced by local injections of viral vectors, the NLGN2 protein was reduced to undetectable level at the infected region.
024278 B6N.129S-Pnpla2tm1Eek/J
Under Development - Now Accepting Orders
The Pnpla2 gene encodes for the adipose triglyceride lipase enzyme that catalyzes the first step in triglyceride hydrolysis. These mice possess loxP sites on either side of exons 2 through 7 of the Pnpla2 gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 7 deleted in the cre-expressing tissues, resulting in loss or significant reduction of ATGL-mediated triacyglycerol hydrolysis in the tissues where Cre recombinase is expressed. Successful tissue-specific Cre-mediated recombination of the floxed ATGL allele in this strain has been verified in cardiac and skeletal muscle (see Stock No. 006475 for example), adipose tissue (see Stock No. 010803), liver (see Stock No. For more information please see the full phenotype on the strain data sheet
024327 B6N.129S4-Ihhtm1Blan/J
Under Development - Now Accepting Orders
These Ihhfl/fl mutant mice possess loxP sites flanking exon 1 of the Indian hedgehog (Ihh) gene. IHH is involved in growth plate maintenance and sustaining trabecular bone after birth. It is also essential for chondrocyte proliferation and differentiation, and osteoblast differentiation during prenatal endochondral bone formation. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissues.

For example, when bred to FVB-Tg(Col2a1-cre/ERT)KA3Smac/J mice (Stock No. 006774), tamoxifen induction resulted in IHH deficient mice which exhibited complete loss of the growth plate and the articular surface, leading to loss of the primary spongiosa, and to impaired bone growth after birth.

023731 B6N.129S6(Cg)-Nkx6-1tm1.1Msan/J
Under Development - Now Accepting Orders
These Nkx6.1flox mutant mice possess loxP sites flanking exon 2 of the NK6 homeobox 1 (Nkx6-1) gene. NKX6.1 is a transcription factor required for the development and maintenance of pancreatic beta cells. Homozygotes are viable and fertile. When bred to mice that express a tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in cre-expressing tissues of the offspring.
024844 B6N.129S6(SJL)-Ubr4tm1.2Nkt/MdnJ
Under Development - Now Accepting Orders
These p600flox/flox mutant mice possess loxP sites flanking exon 1 of the ubiquitin protein ligase E3 component n-recognin 4 (Ubr4) gene. P600 is involved in cytoskeletal organization, membrane morphogenesis, integrin-mediated survival signaling, calcium-calmodulin signaling, and the N-end rule pathway of ubiquitin-proteasome-mediated proteolysis. Embryonic p600 is essential for liver, cardiac, and brain development. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissues.

For example, when bred to B6.Cg-Tg(Sox2-cre)1Amc/J mice (Stock No. 008454) expressing Cre-recombinase under the Sox2 promoter, resulting mice die by E14.5 due to severe cardiac problems including ventricular septal defects and thin ventricular walls. Brain of the knocko .....
For more information please see the full phenotype on the strain data sheet

024344 C.129(B6)-Dhx36tm1.2PmtJpvJ
Under Development - Now Accepting Orders
These RHAUfl/fl mutant mice possess loxP sites flanking exon 8 of the DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36) gene. RHUA is an RNA helicase that plays a role in the deadenylation and decay of mRNAs with 3'-UTR AU-rich elements. RHUA is critical for mouse hematopoiesis. Mice that are homozygous for this allele are viable and fertile. Breeding these mice to mice that express germ line Cre results in embryonic lethality. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 8 deleted in the cre-expressing tissues.

For example, breeding to B6.Cg-Tg(Vav1-cre)A2Kio/J mice (Stock No. 008610) results in a truncated RHAU protein and leads to hemolytic anemia and differentiation defect at the proerythroblast stage.

022017 C.B6-Nuggctm1.2Diaz/J
Under Development - Now Accepting Orders
These Slip-GC floxed mutant mice possess loxP sites flanking exons 2-4, including the highly conserved GTPase domain, of the nuclear GTPase, germinal center associated (Nuggc) gene. Nuggc is expressed in germinal center B cell lymphocytes and may protect B cells from activation-induced deaminase (AID)-dependent DNA breaks. Nuggc is highly expressed in germinal center-derived B cell lymphomas. Homozygotes are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2-4 deleted in cre-expressing tissues.

For example, when crossed to Sox2-cre mice, expressing embryonic Cre recombinase in (see Stock No. 008454), Nuggc deficiency in offspring results in an increase in G:C transitions in B cells from Peyer's Patches.

024638 C57BL/6-Cxcr2tm1Rmra/J
Under Development - Now Accepting Orders
Cxcr2fl/fl floxed mutant mice possess loxP sites flanking exon 2 of the chemokine (C-X-C motif) receptor 2 (Cxcr2) gene. Cxcr2 encodes a G-protein-coupled receptor which mediates neutrophil migration to sites of inflammation. CXCR2 also plays roles in development, tumorigenesis, and demyelination. Homozygotes are viable and fertile. These mice express CXCR2 on the surface of neutrophils in peripheral blood, bone marrow and spleen. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

For example, when these mice are bred to B6.Cg-Tg(Mx1-cre)1Cgn/J mice (Stock No. 003556), poly(I:C)-induced cre activation results in impaired neutrophil migration in sterile peritonitis.

023016 C57BL/6N-Tg(Camk2a-HBEGF)ANkza/J
Under Development - Now Accepting Orders
fDTR-line-A mice have a loxP-flanked alkaline phosphatase-polyadenylation signal upstream of a human diphtheria toxin receptor (DTR; heparin-binding epidermal growth factor-like growth factor (HBEGF)), both under the transcriptional control of the murine CamKIIα promoter. Transgene expression is seen in the forebrain and partial midbrain. Specifically, expression is seen in the dentate gyrus, mossy fibers, and hippocampal area CA1, and hippocampal area CA3. Homozygotes are viable, fertility has not been determined. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will express DTR in cre expressing tissues, making those cells susceptible to diphtheria toxin administration. fDTR-line-A mice have increased DTR expression as compared to C57BL/6N-Tg(Camk2a-HBEGF)ANkza/J mice (Stock No. 023015).
024756 FVB.129S6(Cg)-Foxo1tm1Rdp/J
Under Development - Now Accepting Orders
These Foxo1L/L mutant mice possess loxP sites flanking exon 2 of the forkhead box O1 (Foxo1) gene. FOXO1 is a transcription factor which mediates the transcription of glucose-6-phosphatase, leading to gluconeogenesis and adipogenesis. FOXO1 is involved in the regulation of insulin signaling and metabolic homeostasis, and has been shown to be a tumor suppressor. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 2 deleted in the cre-expressing tissue, resulting in inactivation of Foxo1 gene expression.
024668 FVB.129S6(Cg)-Foxo3tm1Rdp/J
Under Development - Now Accepting Orders
These Foxo3L/L mutant mice possess loxP sites flanking exon 2 of the forkhead box O3 (Foxo3) gene. FOXO3 is a transcription factor which regulates the expression of genes involved in stress resistance, cell cycle progression, tumor progression, longevity, autophagy, and apoptosis. FOXO3 has also been shown to be a tumor suppressor. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 2 deleted in the cre-expressing tissue, resulting in inactivation of Foxo3 gene expression.
024757 FVB.129S6(Cg)-Foxo4tm1Rdp/J
Under Development - Now Accepting Orders
These Foxo4L/L mutant mice possess loxP sites flanking exon 1 of the forkhead box O4 (Foxo4) gene. FOXO4 is a transcription factor involved in the regulation of insulin signaling, and is involved in tumor suppression by promoting cell cycle arrest and apoptosis. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 1 deleted in the cre-expressing tissue, resulting in inactivation of Foxo4 gene expression.
024636 FVB.B6-Tg(CAG-cat,-EGFP)1Rbns/KrnzJ
Under Development - Now Accepting Orders
CAG-CAT-EGFP mice have a loxP-flanked chloramphenicol acetyltransferase (CAT) sequence upstream from an enhanced green fluorescent protein (EGFP) sequence, both under the transcriptional control of a CAG (cytomegalovirus (CMV) early enhancer element and chicken beta-actin) promotor. Heterozygotes are viable and fertile, while homozygotes are viable but infertle. The floxed-CAT sequence blocks transcription of EGFP. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will express EFGP in cre expressing tissues. EGFP fluorescence can be used for tracing multipotent cell lineages.
024760 FVB/N-Tg(Ddx4-cre/ERT2)1Dcas/J
Under Development - Now Accepting Orders
Vasa-CreERT2 mice express Cre-ERT2 directed by the DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (Ddx4) promoter. These mice express Cre-ERT2 protein in the testis and ovary, no expression is seen in other somatic tissues. DXD4 is an RNA helicase involved in germ cell development. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. Mice hemizygous for the Vasa-CreERT2 transgene are viable and fertile. When these mice are bred with mice containing loxP-flanked sequence, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Cre-expressing cells of the offspring.

For example, when bred to B6.129S6(Cg)-Foxo3tm1Rdp/J mice (Stock No. 024668), tamoxifen-inducible cre activation results in .....
For more information please see the full phenotype on the strain data sheet

024714 STOCK Cacna1ctm3Hfm/J
Under Development - Now Accepting Orders
The L-type calcium channels are central regulators of intracellular calcium influx and excitation-contraction coupling in striated muscle, as well as excitability and calcium-mediated signaling in neurons. L-type calcium channel dysregulation has been implicated in pathologies of the heart, such as QT prolongation, as well as skeletal muscle myopathies and neurodegenerative diseases. Cacna1c (calcium channel, voltage-dependent, L type, alpha 1C subunit; also called Cav1.2), encodes the alpha subunit and pore-forming component of the L-type voltage-dependent calcium channel.

Exons 14 and 15 of the mouse Cacna1c gene are flanked by loxP sites in this conditional mutant strain. In the absence of Cre recombinase mice are viable and show no overt phenotype. Cre-mediated recombination produces a frameshift resulting in a premature stop codon and loss of protein. Homozygous knockout embryos die before embryonic day 14.5, but demonstrate a normal heartbeat at embr .....
For more information please see the full phenotype on the strain data sheet

023715 STOCK Dact1tm1.2Bnrc/J
Under Development - Now Accepting Orders
These Dact1flox mice possess loxP sites flanking exon 2 of the dapper homolog 1, antagonist of beta-catenin (Dact1) gene. Dact1 encodes an intracellual protein required for morphogenesis at the primitive streak, and for dendrite, spine, and excitatory synapse development in the mouse forebrain. Through interactions with the Dishevelled family of proteins, DACT1 modulates the transcriptional activation of target genes of the Wnt/beta-catenin signaling pathway as well as beta-catenin-independent branches of Wnt signaling. Homozygotes are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

For example, when bred to B6.129S2-Emx1tm1(cre)Krj/J mice (Stock No. 005628) expressing Cre recombinase in the neurons of the neocortex and hippocampus, neuro .....
For more information please see the full phenotype on the strain data sheet

024843 STOCK Foxi3tm1.1Akg/J
Under Development - Now Accepting Orders
These Foxi3flox mice possess loxP sites flanking exon 2 of the forkhead box I3 (Foxi3) gene. FOXI3 is a transcription factor expressed in pre-placodal ectoderm adjacent to the anterior neural plate, and later in the ectoderm and endoderm lining the pharyngeal arches. FOXI3 is required for the development of the entire inner ear, and for branchial arch neural crest survival, patterning, and the subsequent development of branchial arch derivatives. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

For example, when bred to BALB/c-Tg(CMV-cre)1Cgn/J transgenic mice (Stock No. 003465) expressing Cre recombinase in all tissues, homozygous offspring are embryonic lethal beginning around E9.5, but some homozygous embryos survive until birth .....
For more information please see the full phenotype on the strain data sheet

024107 STOCK Gt(ROSA)26Sortm5(ACTB-tTA)Luo Igs7tm93.1(tetO-GCaMP6f)Hze/HzeJ
Under Development - Now Accepting Orders
Ai93(TITL-GCaMP6f)-D;Rosa26-ZtTA (also called Ai93D;R26-ZtTA) mice have a Cre/Tet-dependent, fluorescent calcium indicator (GCaMP6f; a detector of single neuronal action potentials with fast response kinetics) inserted into the T1 TIGRE intergenic region on chromosome 9, as well as a Cre-dependent tetracycline-controlled transactivator protein (tTA) inserted into the Gt(ROSA)26Sor locus on chromosome 6.

In the absence of Cre recombinase, Ai93D;R26-ZtTA mice are designed to have widespread lacZ expression but no tTA expression. Prior to Cre recombinase, no GCaMP6f expression (EGFP fluorescence) is observed. Following exposure to Cre recombinase, the floxed STOP cassettes in each targeted mutation are deleted; leading to expression of tTA and GCaMP6f in the cre-expressing cells. GCaMP6f expression can be regulated with tetracycline or its analog doxycycline (dox).

When Ai93D;R26-ZtTA mice are bred with Cre-driver lines to create triple transgenic animals (GCaM .....
For more information please see the full phenotype on the strain data sheet

022976 STOCK Igs2tm1(ACTB-EGFP,-tdTomato)Zng/J
Under Development - Now Accepting Orders
Homozygous GT11ML (MADM-ML-11GT) mice are viable and fertile with no gross behavioral or observable abnormalities. The GT11ML allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of mut4-EGFP, a beta-globin intronic sequence (containing frt-lox5171-lox2272-frt-loxP-Neo-loxP), and the MYC-tagged C-terminal portion of tdTomato, all inserted into the Hipp11 locus near the centromere of chromosome 11 (cytoband A1 at ~3cM between the Eif4enif1 and Drg1 loci).
These GT11ML mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to TG11ML mice harboring a reciprocal mutation at the same locus (Stock No. 022977). The resulting GT11/TG11 offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous"), and must also be bred to harbor a Cre- or FLP-recombin .....
For more information please see the full phenotype on the strain data sheet
022977 STOCK Igs2tm2(ACTB-tdTomato,-EGFP)Zng/J
Under Development - Now Accepting Orders
Homozygous TG11ML (MADM-ML-11TG) mice are viable and fertile with no gross behavioral or observable abnormalities. The TG11ML allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of tdTomato, a beta-globin intronic sequence (containing frt-lox5171-lox2272-frt-loxP-Neo-loxP), and the C-terminal portion of mut4-EGFP, all inserted into the Hipp11 locus near the centromere of chromosome 11 (cytoband A1 at ~3cM between the Eif4enif1 and Drg1 loci).
These TG11ML mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to GT11ML mice harboring a reciprocal mutation at the same locus (Stock No. 022976). The resulting GT11/TG11 offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous"), and must also be bred to harbor a Cre- or FLP-recombinase to induc .....
For more information please see the full phenotype on the strain data sheet
022143 STOCK Il7rtm1.1Asin/J
Under Development - Now Accepting Orders
The IL7 receptor is important in the regulation of T, B, and NK lymphoid cell development. The Il7r, interleukin 7 receptor, gene encodes a subunit of the IL-7 receptor, which is essential for T cell development and V(D)J recombination. These IL-7Rfl mice possess loxP sites flanking exon 3 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in preselection double positive thymocytes, this mutant mouse strain may be useful in studies of T cell population subset differentiation in the thymus.

023406 STOCK Tfap2atm2Will/J
Under Development - Now Accepting Orders
These floxed-AP-2alpha mutant mice possess loxP sites flanking exons 5-6 of the transcription factor AP-2α gene. AP-2alpha is expressed in the neural tube, and in cranial and cardiac neural crest cells in the developing embryo, as well as in the progress zone of the limb bud, the developing kidney, the eye, and surface ectoderm. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 5-6 deleted in the cre-expressing tissues.

For example, when bred to B6.Cg-Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J mice (Stock No. 009107) expressing Cre recombinase in the developing neural tube, offspring are perinatal lethal due neural tube closure defects and cleft secondary palate. Some mutant mice can survive into adulthood and exhibit retarded craniofacial growth, abnormal middle ear development, and de .....
For more information please see the full phenotype on the strain data sheet

023408 STOCK Tfap2ctm2Will/J
Under Development - Now Accepting Orders
These floxed-AP-2gamma mutant mice possess loxP sites flanking exon 6 of the transcription factor AP-2, gamma (Tfap2c) gene. AP-2gamma is a transcription factor expressed early in embryogenesis in trophoblast cell lineage. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 6 deleted in the cre-expressing tissues.
023592 STOCK Vapbtm1.2Ics/J
Under Development - Now Accepting Orders
These Vspb-/- mutant mice lack exon 3 of the vesicle-associated membrane protein, associated protein B and C (Vapb) gene, abolishing gene expression. Vspb encodes a membrane protein involved in vesicle trafficking, membrane fusion, protein complex assembly, and cell motility. Mutations and loss of VAPB function have been shown to contribute to motor deficits associated with amyotrophic lateral sclerosis (ALS) pathogenesis. Homozygotes are viable and fertile. Adult Vapb KO mice develop mild, late onset central nervous system defects including mild motor deficits after 18 months of age and mild tremors in open field tests. These mice do not exhibit defects in neuromuscular junction or muscle strength, and there is no apparent muscle denervation.
006373 129-Braftm1Sva/J
Cryopreserved - Ready for recovery
Homozygous "floxed B-raf" (B-raff/f) mice are viable and fertile with normal B-raf protein expression. When bred to mice expressing Cre recombinase under the control of a promoter of interest, exon 12 of the targeted gene is deleted in the tissue of interest. These mutant mice may be useful in neurological studies such as Ras/Raf and MEK/ERK signaling, synaptic (neural) plasticity, learning and memory.

For example, when crossed to a strain expressing Cre recombinase in nervous tissue (see Stock No. 003771), this mutant mouse strain may be useful in studies of neuron development.

For example, when crossed to a strain expressing interferon inducible Cre recombinase (see Stock No. 003755), this mutant mouse strain may be useful in studies of extraembryonic mammmalian development.

006835 129-Dag1tm2Kcam/J
Cryopreserved - Ready for recovery
Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express cre recombinase, resulting offspring can have exon 2 of the targeted gene deleted in the cre-expressing tissue(s). As the targeted gene has three loxP sites, other genotypes may also result. These mutant mice may be useful in studying muscle disease and regeneration.

When bred to a strain expressing Cre recombinase under the control of the human glial fibrillary acidic protein promoter (GFAP) (see Stock No. 004600 for example), this mutant mouse strain may be useful in studies of brain abnormalities observed in congenital muscular dystrophies.

When bred to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771 for exampl .....
For more information please see the full phenotype on the strain data sheet

008669 129-Daxxtm2Led/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon II (also called exon 3, when the first non-coding exon is included in the numbering scheme) of the targeted gene. Mice that are homozygous for this allele are viable and fertile. Sequence analysis revealed a single base deletion mutation in the first loxP site. When these mutant mice are bred to mice that express Cre recombinase, recombination efficiency is weak and resulting offspring may not have exon 2 deleted in cre-expressing tissue(s). When primary cell cultures derived from this strain (cortical neurons) are infected with lentiviral particles expressing the Cre recombinase, exon II is deleted. These mice are are an effective tool for achieving cre recombination but only in an ex vivo context.
006053 129-Gt(ROSA)26Sortm1(CAG-EGFP)Luo/J
Cryopreserved - Ready for recovery
MADM-GG mice are viable with no gross behavioral or observable abnormalities. Regardless of Cre-recombination, these mice express EGFP as their N- and C-terminal coding sequences are interrupted by the beta-actin intron in-frame. High EGFP expression in every cell can be visualized in vivo and in fixed samples. These mutant mice are a control EGFP-expressing strain for use with MADM (mosaic analysis with double markers) mice (see Stock No. 006041 [MADM-GR (EGFP/Dsred2)] and Stock No. 006067 [MADM-RG (Dsred2/EGFP)]). Using the MADM system, a researcher can generate genetic mosaics in which an individual organism contains somatic cells of different genotypes. This allows the researcher to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation and mitosis.

Mice harbor .....
For more information please see the full phenotype on the strain data sheet

006067 129-Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/J
Cryopreserved - Ready for recovery
MADM-RG mice are viable with no gross behavioral or observable abnormalities. Homozygous females produce less pups to weaning age compared to heterozygotes. These mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to mice harboring a reciprocal mutation at the same locus (see Stock No. 006041 or Stock No. 006075, MADM-GR (EGFP/Dsred2)). The resulting offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous") and must next be bred to a Cre-expressing strain for fluorescent protein expression. Prior to Cre-recombination, double mutant mice do not have colored cells: the chimeric genes do not produce functional proteins because their coding sequences are interrupted by the beta-actin intron in different reading frames. After DNA replication (G2 phase) in double mutant mice, Cre-recombinase introducti .....
For more information please see the full phenotype on the strain data sheet
006041 129-Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo/J
Cryopreserved - Ready for recovery
MADM-GR mice are viable with no gross behavioral or observable abnormalities. Homozygous mice have low fertility, while heterozygous mice have no reported fertility defects. These mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to mice harboring a reciprocal mutation at the same locus (see Stock No. 006067 or Stock No. 006080, MADM-RG (Dsred2/EGFP)). The resulting offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous") and must next be bred to a Cre-expressing strain for fluorescent protein expression. Prior to Cre-recombination, double mutant mice do not have colored cells: the chimeric genes do not produce functional proteins because their coding sequences are interrupted by the beta-actin intron in different reading frames. After DNA replication (G2 phase) in double mutant mice, Cre-recom .....
For more information please see the full phenotype on the strain data sheet
021985 129S(Cg)-Tbxa2rtm1.1Bhk/J
Cryopreserved - Ready for recovery
005109 129S(FVB)-Men1tm1.2Ctre/J
Cryopreserved - Ready for recovery
These mice possess loxP sites flanking exons 3 to 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

When bred to a strain expressing Cre recombinase in parathyroid tissue (see Stock No. 005989 for example), this mutant mouse strain may be useful in studies of hypercalemia and parathyroid neoplasia.

007664 129S-Efnb1tm1Sor/J
Cryopreserved - Ready for recovery
These mice possess loxP sites flanking exons 2 through 5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 5 deleted in the cre-expressing tissue(s). These Efnb1 conditional mutant mice may be useful in studying cellular signaling in embryonic development and adult mice; specifically receptor tyrosine kinases.

For example, when crossed to a strain expressing Cre recombinase in epiblast-derived tissues (see Stock No. 003755), this mutant mouse strain may be useful in embryogenesis research.

For example, when bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 007807 or For more information please see the full phenotype on the strain data sheet

003310 129S-Gt(ROSA)26Sortm1Sor/J
Cryopreserved - Ready for recovery
Mice heterozygous or homozygous for the Gtrosa26tm1Sor targeted mutation may be used to test the tissue/cellular expression pattern of the cre transgene in any transgenic strain carrying cre under the regulation of a specific promoter. Cre expression results in the removal of a loxP-flanked DNA segment that prevents expression of a lacZ gene. When crossed with a cre transgenic strain, lacZ is expressed in cells/tissues where cre is expressed. The 129-Gtrosa26tm1Sor strain is particularly useful for this purpose because the ROSA26 promoter leads to generalized expression of lacZ during development or in the adult.
009043 129S-Gt(ROSA)26Sortm3(CAG-luc)Tyj/J
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In the absence of Cre, there is low level expression of Luciferase-SIY H2b antigen expression. After Cre activity, the recombined locus has high expression of Luciferase-SIY fusion protein. This mutant mouse strain may be useful in studies of immune response to a self-antigen or over-expressed tumor-associated antigen when used in combination with tumor-prone models. This mouse is also useful as a reporter for Cre activity.
017340 129S-Maoatm1Shih/J
Cryopreserved - Ready for recovery
These MAO-Aneo mutant mice possess a single loxP site downstream of exon 11, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 12 of the X-linked monoamine oxidase A (Maoa) gene. Hemizygous males and homozygous females are viable, fertile, and normal in size. MAO-A is a mitochondrial enzyme that oxidizes monoamine neurotransmitters and dietary monoamines. Insertion of the floxed-neo cassette results in a truncation of the C-terminal of MAO-A resulting in expression of a hypomorphic MAO-A transcript in the prefrontal cortex and amygdala. These mice exhibit an increase of pyramidal neuron dendritic length in the orbitofrontal cortex, a reduction in basilar dendritic length, and an increase in apical dendritic length. These mice display reduction in social interaction, reduction in anxiety like behaviors, and reduction in total locomotor activity.

When these mutant mice are bred to mice that express Cre recombinase, re .....
For more information please see the full phenotype on the strain data sheet

009084 129S-Mnttm1Awb/J
Cryopreserved - Ready for recovery
These mice possess loxP sites flanking exons 4-6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 4 through 6 deleted, the loxP site flanked hygro resistance cassette deleted, or both excised in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in a wide range of tissues (see Stock No. 003314 for example), this mutant mouse strain may be useful in studies of Miller-Dieker syndrome, embryonic development and craniofacial defects.

When bred to a strain with Cre recombinase expression in a wide range of tissues, especially mammary gland (see Stock No. 003553 for example), this mutant mouse .....
For more information please see the full phenotype on the strain data sheet

008002 129S-Pafah1b1tm2Awb/J
Cryopreserved - Ready for recovery
These mice possess loxP sites flanking exons 3 through 6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Histological analysis of brains from homozygotes reveals slightly wider CA1 and CA3 regions and a split in CA2 region in the hippocampus. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3 through 6 deleted in the cre-expressing tissue(s).
017789 129S-Slc11a2tm2Nca/J
Cryopreserved - Ready for recovery
The divalent metal transporter DMT1, encoded by Slc11a2, is essential for iron and micronutrient uptake in the gut and other tissues. These mice possess loxP sites on either side of exons 6 through 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 6 through 8 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in hippocampal neurons, this mutant mouse strain may be useful in studies of the role of iron in neurodevelopment, neurobiology and neurodegenerative diseases.

008396 129S-Top2btm2Jcw/J
Cryopreserved - Ready for recovery
These mice possess loxP sites flanking the 3 exons encoding the active-site tyrosyl residue of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the 3 exons deleted in the cre-expressing tissue(s).

When bred to a strain expressing Cre recombinase during spermatogenesis (see Stock No. 003328 for example), this mutant mouse strain may be useful in studies of neural development.

When bred to a strain expressing Cre recombinase in the telencephalon and discreet head structures (see Stock No. 004337, 006084 for example), this mutant mouse strain may be useful in studies of corticogenesis.

008652 129S-Trp53tm2Tyj/J
Cryopreserved - Ready for recovery
These mice carry a conditional point-mutant allele of the transformation related protein 53 gene (p53R172H; structural mutant homologous to human p53 codon 175). The conditional allele is functionally equivalent to a null mutation. Mice have all the phenotypic issues of p53 knockout mice and therefore have some decreased viability of homozygotes. Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.
008651 129S-Trp53tm3Tyj/J
Cryopreserved - Ready for recovery
These mice carry a conditional point-mutant allele of the transformation related protein 53 gene (p53R270H; contact mutant homologous to human p53R273H). The conditional allele is functionally equivalent to a null mutation. Mice have all the phenotypic issues of p53 knockout mice and therefore have some decreased viability of homozygotes. Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.
008649 129S-Trpa1tm2Kykw/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of the S5/S6 transmembrane domains of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, the S5/S6 transmembrane domains of the targeted gene are deleted in the tissue of interest. This strain may be useful in functional studies of cation channels.
008180 129S/Sv-Krastm4Tyj/J
Cryopreserved - Ready for recovery
This strain carries a point mutation (G12D) whose expression is blocked by the presence of a loxP-flanked stop codon. Homozygotes die in utero. Cre-mediated recombination can excise the stop codon and permit the oncogenic protein to be expressed. Intranasal infection with an adenovirus encoding Cre results in a very high frequency of lung tumors and permits controlled timing of tumor initiation and tumor multiplicity. This strain may be useful in studies of cancer and development.

When bred to a strain expressing Cre recombinase under the control of a tetracycline-responsive promoter element and a strain expressing a tetracycline-controlled activator protein in lung epithelial cells (see Stock No. 006234 and 006235 respectively), this mutant mouse strain may be useful in studies of lung development.

When bred to a strain expressing Cre recombinase in th .....
For more information please see the full phenotype on the strain data sheet

010977 129S4/SvJae-Pdgfrbtm11Sor/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of the exons encoding the first and second immunoglobulin domain of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the exons encoding the first and second immunoglobulin domain deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in adrenal tissue (see Stock No. 006364 for example), this mutant mouse strain may be useful in studies of steroidogenesis.

013184 129S4/SvJaeSor-Mycltm1.1Rne/J
Cryopreserved - Ready for recovery
These L-Mycflox mutant mice possess loxP sites flanking all three exons v-myc myelocytomatosis viral oncogene homolog 1 (Mycl1) targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have all three Mycl1 exons deleted in the cre-expressing tissue. The donating investigator states that this strain may be useful, in combination with other Myc knock-out models, for studying early embryonic growth and organogenesis of the brain, olfactory bulb, and lungs.
021932 B6(129S4)-Cacna1gtm1Stl/J
Cryopreserved - Ready for recovery
The Cacna1g gene encodes for a T-type calcium channel, which is a low-voltage activated channel and is expressed throughout the brain. These mice possess loxP sites flanking exons 2 through 12 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have 2 through 12 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in thalamic projection neurons (see Stock No. 008582 for example), the resulting mice exhibit a fragmented sleep pattern.

017762 B6(Cg)-Cacna1ftm1.2Sdie/J
Cryopreserved - Ready for recovery
The mouse I756T point mutation is equivalent to an I745T mutation in the human CACNA1F gene that is responsible for an inherited retinal disorder related to, but distinct from, X-linked incomplete congenital stationary night blindness in a New Zealand family. These mice carry the I756T point mutation in exon 17, that results in an amino acid substitution of threonine for isoleucine at position 756, and loxP sites flanking exons 14 through 17. Female mice that are homozygous and male mice that are hemizygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator reports that this targeted mutation is a gain-of-function allele in an in vitro expression system. This strain was generated from breeding (Stock No. 017760) mice to a FLP recombinase expressing strain.
007694 B6(Cg)-Cd79btm168Mnz/J
Cryopreserved - Ready for recovery
Mice homozygous for this targeted insertion are viable and fertile and do not display any gross physical or behavioral abnormalities. This strain carries a targeted insertion of an 18 bp I-SceI (S. cerevisiae) endonuclease recognition site in intron 2 of the gene. Infection of B lymphocytes with a retrovirus encoding for the I-SceI meganuclease produces Igh (immunoglobulin heavy chain )-IgB (Cd79b) chromosomal translocations with breakpoints at the I-SceI sequence on the mutant allele.
017564 B6(Cg)-Crbntm1.1Jjh/J
Cryopreserved - Ready for recovery
Crbn (cereblon) is a target of immunomodulatory drugs, such as thalidomide, and plays an important role in central nervous system development. A nonsense mutation in the human cereblon gene is the cause of a type of autosomal recessive non-syndromic intellectual disability. These mice possess loxP sites on either side of exons 3 and 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3 and 4 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in the forebrain (see Stock No. 005359 for example), this mutant mouse strain may be useful in studies of non-syndromic mental retardation, memory and learning.

024119 B6(Cg)-Hdac3tm1.1Laz/J
Cryopreserved - Ready for recovery
These Hdac3flox/flox mutant mice possess loxP sites flanking exons 4-7 of the histone deacetylase 3 (Hdac3) gene. HDAC3 is an enzyme that regulates the acetylation of histone tails by modulating histone-DNA interactions resulting in gene silencing. HDAC3-induced silencing is involved in cell growth, apoptosis, neuronal plasticity, tumor suppression, transcriptional regulation, cell cycle progression, and development. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 4-7 deleted in the cre-expressing tissues.

For example, 2 weeks after infusion of adenoviral Cre recombinase in area CA1 of the dorsal hippocampus, HDAC3 deficiency results in enhanced long term memory.

When bred to B6.Cg-Tg(Fabp4-cre)1Rev/J mice (Stock No. 005069) expressing Cre recombinase in brown .....
For more information please see the full phenotype on the strain data sheet

017905 B6(Cg)-Lair1tm1Jco/J
Cryopreserved - Ready for recovery
LAIR1, an inhibitory receptor protein with a high affinity for collagen, is expressed on most cells of the immune system and is associated with inhibition of the cytotoxic activity of NK and CD8+ T cells, BCR-induced B cell activation and proliferation, and CD3 signaling and cytokine production by T cells. These mutant mice possess loxP sites flanking exons 4 to 8 of the (Lair1) gene. Mice that are homozygous for this allele are viable, fertile, and normal in size. When bred to mice that express Cre recombinase, resulting offspring will have exons 4-8 deleted in the cre-expressing tissues. This strain may be useful for studying immune cell regulation.
017866 B6(Cg)-Mc3rtm1Butl/J
Cryopreserved - Ready for recovery
These Mc3r-/- mutant mice possess a floxed STOP-neo cassette upstream of the start codon, and an IRES-GFP cassette downstream of the stop codon of the melanocortin 3 receptor (Mc3r) gene. MC3R is a G-protein coupled receptor for the melanocyte-stimulating hormone. It is expressed in the hypothalamic and limbic regions of the brain and in some peripheral tissues including the renal cortex and medulla, and the immune system. MC3R contributes to the regulation of body composition, salt excretion, immune function, and energy homeostasis. Homozygous MC3R-/- males are fertile while homozygous females are poor breeders. These floxed-STOP mice exhibit increased susceptibility to diet-induced obesity, with increased fat mass and decreased lean mass when fed a standard chow diet. These mice can also exhibit increased circulating insulin and leptin levels, changes in serum triglyceride levels, changes in the respiratory exchange ratio (indicating altered whol .....
For more information please see the full phenotype on the strain data sheet
014601 B6(Cg)-Noxa1tm1Brg/BrgJ
Cryopreserved - Ready for recovery
These mice possess loxP sites flanking exons 3 to 6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3 to 6 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in epiblast-derived tissues of the early embryo(see Stock No. 003755 for example), this mutant mouse strain may be useful in studies of phagocytic cells and chronic granulomatous diseases.

017889 B6(Cg)-Shank3tm1.1Bux/J
Cryopreserved - Ready for recovery
The Shank3flox ex4-9 allele has loxP sites flanking exons 4-9 of the SH3/ankyrin domain gene 3 gene (Shank3). When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the ankyrin repeat domains deleted in the cre-expressing tissues. As SHANK3 is necessary for forming functional glutamatergic synapses between receptors and cytoskeletal/scaffolding elements, these floxed mice may be used to generate tissue-specific Shank3 deletions for studying synaptic glutamate receptor development/function, transmission and plasticity, as well as Shank3 haploinsufficiency, neurobehavioral manifestations of 22q13 deletion syndrome and/or autism spectrum disorders.
008118 B6(Cg)-Snord116tm1Uta/J
Cryopreserved - Ready for recovery
Mice homozygous for this 2-loxP (floxed) allele are viable and fertile, with loxP sites flanking the Snord116 small nucleolar RNAs (snoRNAs) gene cluster. When bred to mice that express Cre recombinase, the resulting offspring will have this gene cluster deleted in the cre-expressing tissue(s). Because the Snord116 gene cluster is imprinted and only expressed from the paternal allele, breeding 2-loxP males with cre-expressing females may be required to generate deleted offspring with the knockout phenotype. The donating investigator reports that the distance between the two loxP sites (~140 kb) may reduce the recombination efficiency in somatic cells. As deletions of the Snord116 cluster are associated with Prader-Willi syndrome (PWS), mice carrying the 2-loxP (floxed) allele may be useful in generating conditional mutations for studying the role of Snord116 in growth and feeding regulation, mechanisms of obesity, and patho .....
For more information please see the full phenotype on the strain data sheet
007665 B6.129(C3)-Vcam1tm2Flv/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of the cytokine-responsive promoter region and exon 1 of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene (widespread deletion of expression is lethal).

For example, when crossed to a strain expressing interferon inducible Cre recombinase (see Stock No. 003556), this mutant mouse strain may be useful in studies of bone marrow nonhematopoietic cells.

When crossed to a strain expressing Cre recombinase in endothelial cells (see Stock No. 004128), this mutant mouse strain may be useful in studies of lymphocyte migration.

018057 B6.129(Cg)-Cul7tm2.1Jdec/J
Cryopreserved - Ready for recovery
The CUL7 protein is a component of cullin-RING ligase (SKP1-cullin-F box protein complex) that is part of the ubiquitin-proteasome pathway of protein degradation. These mice possess loxP sites on either side of exons 2 through 4 of the Cul7 (cullin 7) gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 4 deleted in the cre-expressing tissues. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6 genetic background.

When bred to a strain with early embryonic Cre recombinase expression (see Stock No. 003724 for example), this mutant mouse strain may be useful in studies of placental growth, embryonic development, and neonatal lethality.

009652 B6.129(Cg)-Dag1tm2.1Kcam/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 2 of the targeted gene is deleted in the tissue of interest.
018132 B6.129(Cg)-Itm2btm1Ldad/J
Cryopreserved - Ready for recovery
These BRI2f/f floxed mutant mice possess loxP sites flanking exon 2 of the targeted integral membrane protein 2B (Itm2b) gene. The accumulation of cleaved amyloid β-peptide (Aβ) precursor proteins (APP) form amyloid plaques in the brains of patients with familial dementias such as Alzheimers disease. BRI2 is a membrane protein which binds to APP. When over-expressed, BRI2 inhibits the accumulation of Aβ in vitro. Also, mutations in BRI2 are associated with familial British dementia (FBD) and familial Danish dementia (FDD). Homozygous BRI2f/f mice are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in cre-expressing tissues, abolishing gene expression. BRI2 deficiency results in severe hippocampal memory deficits.
004318 B6.129(FVB)-Gabra1tm1Geh/J
Cryopreserved - Ready for recovery
These mice possess loxP sites flanking the second transmembrane domain of the gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1 (Gabra1) gene. GABA is an inhibitory neurotransmitter in the nervous system which helps regulate neuronal excitability. Mice that are homozygous for this floxed Gabra1 allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of Gabra1.

For example, when these mice are bred to B6.C-Tg(CMV-cre)1Cgn/J mice (Stock No. 006054) expressing cre in all tissues, the resulting double mutant mice exhibit sex-dependent decreased survival after P19, decreased body mass, EEG spike-wave discharges, and seizures. Heterozygotes display an intermediate decrease in body mass and survival. <> .....
For more information please see the full phenotype on the strain data sheet

012941 B6.129(SJL)-Foxn1tm1.1Dmsu/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 5 and 6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. RT-PCR analysis of thymic cells isolated from homozygotes reveals the gene product (mRNA) levels are the same as wildtype control. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 5 and 6 deleted in the cre-expressing tissue(s).

When bred to a strain with inducible widespread Cre recombinase expression (see Stock No. 004682 for example), this mutant mouse strain may be useful in studies of thymic epithelial differentiation and T cell development.

For example, when bred to mice carrying Tg(KRT5-cre/ERT2)2Ipc (see Stock No. 018394 tamoxifen-induced Cre-mediated recombi .....
For more information please see the full phenotype on the strain data sheet

008471 B6.129(SJL)-Oxtrtm1.1Wsy/J
Cryopreserved - Ready for recovery
Mice homozygous for the Oxtrflox allele are viable and fertile, with loxP sites flanking exons 2-3 of the targeted gene. Expression and receptor binding distributions from the Oxtrflox targeted allele are reported to be normal when compared to wild-type. When Oxtrflox mice are bred to mice that express Cre recombinase, the resulting offspring will have the floxed region deleted in the cre-expressing tissue(s). These Oxtrflox mice may be used for spatial and temporal inactivation of the oxytocin receptor in studying (for example) parturition and lactation, as well as social, behavioral, and learning disorders such as autism and anxiety.

These Oxtrflox mice may also be useful along with the Oxt/EGFP AI03 transgenic mice (Stock No. 006043) or oxytocin targeted mutant mice (Stock No. 002713) from the same .....
For more information please see the full phenotype on the strain data sheet

016159 B6.129-Adartm1Knk/Mmjax
Cryopreserved - Ready for recovery
Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Widespread excision of the floxed fragment in homozygous mutants during embryogenesis results in embryonic lethality between E11 and E12.5. Widespread apoptosis is detected in tissues at E10.5 to E11.5, however, embryos appear grossly normal with the exception of occasional pallor and reduced size. This mutant strain is useful in organ specific studies involving embryogenesis and stress induced apoptosis.

Heterozygote: Normal

013195 B6.129-Aiptm2Bra/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 3 through 6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3 through 6 deleted in the cre-expressing tissue(s).
017745 B6.129-Anapc2tm1Hez/J
Cryopreserved - Ready for recovery
Anaphase promoting complex subunit 2 is involved in cell cycle regulation and patterning during embryonic development. These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in neuronal cells, Western blot analysis of brain tissue confirmed a significant decrease in Anapc2 expression.

005319 B6.129-Cdh1tm2Kem/J
Cryopreserved - Ready for recovery
These mice possess loxP sites flanking exons 6 to 10 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.
016920 B6.129-Chattm1Jrs/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 3 and 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3 and 4 deleted in the cre-expressing tissue(s). The Donating Investigator reports that the presence of the FRT site flanked PGK-Neo cassette has no detectable effect on gene expression in this strain.

When bred to a strain with Cre recombinase expression in retinal cells, this mutant mouse strain may be useful in studies of developing neural circuits in the retina.

016183 B6.129-Csnk1etm1Asil/J
Cryopreserved - Ready for recovery
The tau mutation of Syrian hamsters was the first mammalian circadian mutation discovered. These targeted mice carry the tau mutation in exon 4 of the mouse Csnk1e (casein kinase 1, epsilon; also called Ck1 epsilon) gene. Homozygotes show a shortened circadian period of behavior (in vivo) and suprachiasmatic nucleus firing rates (in vitro) due to accelerating Per1 (period homolog 1 (Drosophila))-dependent molecular feedback loops. LoxP sites located on either side of the mutant exon 4 enable tissue-specific cre excision of the catalytic domain to knock out expression. Homozygotes are viable and fertile with no overt deleterious phenotype.
018059 B6.129-Cul9tm2Jdec/J
Cryopreserved - Ready for recovery
Cullin 9, also known as PARC (PARkin-like cytoplasmic protein), is involved in the post-translational protein modification processes ubiquitination and neddylation through interactions with both NEDD8 and RBX1. These mice possess loxP sites on either side of exons 4 through 6 of the cullin 9 (Cul9) gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 4 through 6 deleted in cre-expressing tissues. These mice carry a FRT site flanked NEO cassette. The Donating Investigator reports that this NEO cassette inhibits expression of the (Cul9) gene. When bred to FLP recombinase expressing mice, resulting offspring will exhibit a wildtype level of (Cul9)expression. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6 genetic background.

When bred to a strain with early embryonic Cre recombinase expressi .....
For more information please see the full phenotype on the strain data sheet

006834 B6.129-Dag1tm2Kcam/J
Cryopreserved - Ready for recovery
Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a Cre recombinase gene under the control of a promoter of interest, exon 2 of the targeted gene and a neomycin cassette are deleted in the tissue of interest. These mutant mice may be useful in studying muscle disease and regeneration.

When bred to a strain expressing Cre recombinase under the control of the human glial fibrillary acidic protein promoter (GFAP) (see Stock No. 004600 for example), this mutant mouse strain may be useful in studies of brain abnormalities observed in congenital muscular dystrophies.

When bred to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771 for example), this mutant mouse strain may be useful in studies of retinal physiology. .....
For more information please see the full phenotype on the strain data sheet

006887 B6.129-Dpagt1tm2Jxm/J
Cryopreserved - Ready for recovery
Mice homozygous for this floxed targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exon is deleted by cre expression to produce a null allele.
019097 B6.129-Fktntm1Kcam/J
Cryopreserved - Ready for recovery
Fktn (fukutin) is one of several genes that are required for dystoglycan processing. Deficiencies in dystroglycan are associated with an array of congenital and limb girdle muscular dystrophies (dystroglycanopathies).

These mice carry a floxed allele of Fktn. Homozygotes are born at reduced Mendelian frequencies and have reduced fertility. Viable animals have an overtly normal phenotype. Dystroglycan is properly glycosylated in all tissues tested.

When crossed with a Cre strain, tissue-specific or inducible knockouts of the gene can be generated. Crosses with Myf5 (myogenic factor 5) (Stock No. 007893) and Ckm (creatine kinase, muscle) (Stock No. 006405) Cre strains generate dystroglycanopathy mice representative of a spectrum of mild to severe patient diseases. Conditional disruption during muscle development at E8 causes a more sev .....
For more information please see the full phenotype on the strain data sheet

006874 B6.129-Gabra4tm1.2Geh/J
Cryopreserved - Ready for recovery
Mice homozygous for this GABAA-R alpha4F allele are viable and fertile. These mutant mice have loxP sites flanking exon 3 of the targeted gene. When bred to Cre-recombinase expressing mice, offspring will have a deletion of exon 3 in the cre expressing tissue(s). These "floxed" mice may be useful in neurological studies including behavior and neurotransmitter function.

Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations are available from this donating investigator (Dr. Gregg Homanics, University of Pittsburgh), including Gabra1 (Stock No. 004318), Gabra4 (Stock No. 006874), Gabra6 (Stock No. 002710), Gabrb3 (Stock No. 002711), Gabrd (Stock No. .....
For more information please see the full phenotype on the strain data sheet

006896 B6.129-Galnt13tm1Jxm/J
Cryopreserved - Ready for recovery
Mice homozygous for the floxed targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele. This glycosyltransferase is also sometimes termed as ppGalNAcT-13 in studies of human tissues.
006895 B6.129-Galnt1tm1Jxm/J
Cryopreserved - Ready for recovery
Mice homozygous for this floxed targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exon is deleted by cre expression to produce a null allele.
021329 B6.129-Gbatm1Clk/J
Cryopreserved - Ready for recovery
Inherited deficiency of the lysosomal hydrolase, glucocerebrosidase (encoded by Gba, glucosidase, beta, acid) underlies the autosomal recessive disorder, Gaucher disease. Excessive glucocerebroside storage may lead to the associated tissue dysfunctions. Affected individuals present with a spectrum of clinical symptoms ranging from hepatosplenomegaly, haematological abnormalities, and bone pain, to severe neurodegeneration and premature death. Homozygous knockouts of Gba in mice are perinatal lethal.

In this targeted allele, exons 9-11 are flanked by loxP sites enabling tissue-directed knockouts of the locus via Cre excision. Incomplete expression of Tie2Cre-recombinase (see Stock No. 004128) in cells of hematopoietic and endothelial origin results in deficiency of glucocerebrosidase in the liver, spleen, bone marrow, and peripheral white cells. Glucocerebroside storage in this model leads to progressive sple .....
For more information please see the full phenotype on the strain data sheet

006886 B6.129-Gcnt1tm1Jxm/J
Cryopreserved - Ready for recovery
Mice homozygous for the mutation are viable, fertile and do not display any gross physical or behavioral abnormalities. The mice are devoid of significant gene-encoded enzyme activity in spleen, bone marrow, and kidney. They exhibit a moderate increase in the number of neutrophils in the blood, a partial deficiency of selectin ligands, and a mild B cell homing deficit. Neutrophil rolling is reduced on E-, L- and P-selectin substrates.
006924 B6.129-Gcnt3tm1Jxm/J
Cryopreserved - Ready for recovery
Mice that are homozygous for the floxed targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele.
006925 B6.129-Gcnt4tm1Jxm/J
Cryopreserved - Ready for recovery
Mice homozygous for this floxed targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to Cre transgenic strains, the loxP-flanked exons are deleted by Cre expression to produce a null allele.
006071 B6.129-Gt(ROSA)26Sortm1(CAG-EGFP)Luo/J
Cryopreserved - Ready for recovery
Homozygous and heterozygous mutant mice are viable and fertile, and manifest no gross behavioral or phenotypic abnormalities. These mutant mice carry an EGFP construct in which the N- and C-terminal coding sequences are interrupted by the beta-actin intron in-frame. Despite the presence of this intron, high EGFP expression in every cell can be visualized in vivo and in fixed tissues.

These mutant mice were designed as an EGFP-expressing control strain for use with MADM (mosaic analysis with double markers) mice (See Stock No. 006041 [EGFP/Dsred2] and Stock No. 006067 [Dsred2/EGFP]). Using the MADM system, a researcher can generate genetic mosaics in which an individual organism contains somatic cells of different genotypes. This allows the researcher to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be .....
For more information please see the full phenotype on the strain data sheet

007708 B6.129-Gt(ROSA)26Sortm1(HD*103Q)Xwy/J
Cryopreserved - Ready for recovery
Mice heterozygous for the RosaHD mutant allele are viable and fertile. These mice have the neuropathogenic polyQ-mutant variant of the human Huntingtin protein (mhtt-exon1; 103Q) inserted into the Gt(ROSA)26Sor locus. Expression of mhtt-exon1 is blocked by an upstream loxP-flanked transcriptional STOP sequence. When bred to mice with a Cre recombinase gene under the control of a promoter of interest, the STOP sequence is deleted in the tissue of interest, and mhtt-exon1 expression is observed. As these RosaHD mutant mice allow cre-conditional expression of the neuropathogenic mhtt-exon1 protein, they may be useful in studying Huntington's disease (HD) or other polyQ disorders. Of note, sequencing of the polyQ region (using mice from the 11th backcross) indicate the actual number of repeats to be 98.

For example, when bred to strains expressing cre in brain tissues (such as Nestin-Cre (see Stock No. 003771 .....
For more information please see the full phenotype on the strain data sheet

006080 B6.129-Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/J
Cryopreserved - Ready for recovery
MADM-RG mice are viable with no gross behavioral or observable abnormalities. Homozygous females produce less pups to weaning age compared to heterozygotes. These mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to mice harboring a reciprocal mutation at the same locus (see Stock No. 006041 or Stock No. 006075, MADM-GR (EGFP/Dsred2)). The resulting offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous") and must next be bred to a cre-expressing strain for fluorescent protein expression. Prior to Cre recombination, double mutant mice do not have colored cells: the chimeric genes do not produce functional proteins because their coding sequences are interrupted by the beta-actin intron in different reading frames. After DNA replication (G2 phase) in double mutant mice, Cre recombinase int .....
For more information please see the full phenotype on the strain data sheet
006075 B6.129-Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo/J
Cryopreserved - Ready for recovery
MADM-GR mice are viable with no gross behavioral or observable abnormalities. Homozygous mice have low fertility, while heterozygous mice have no reported fertility defects. These mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to mice harboring a reciprocal mutation at the same locus (see Stock No. 006067 or Stock No. 006080, MADM-RG (Dsred2/EGFP)). The resulting offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous") and must next be bred to a Cre-expressing strain for fluorescent protein expression. Prior to Cre-recombination, double mutant mice do not have colored cells: the chimeric genes do not produce functional proteins because their coding sequences are interrupted by the beta-actin intron in different reading frames. After DNA replication (G2 phase) in double mutant mice, Cre-recom .....
For more information please see the full phenotype on the strain data sheet
009591 B6.129-Kcnn1tm1.2Jpad/J
Cryopreserved - Ready for recovery
Homozygous SK1-flox (delta neo) mice are viable and fertile, with loxP sites flanking the coding sequences of exons 3-5 (and an enhanced green fluorescent protein (EGFP) coding sequence just downstream of the second loxP site) in the Kcnn1 (also called SK1) locus. The donating investigator reports that homozygotes exhibit no overt phenotype and that the SK1-flox (delta neo) allele may confer a null phenotype even before exposure to Cre recombinase: RT-PCR shows the EGFP sequence disrupts splicing such that no full length transcripts are detected. No EGFP expression is reported. When bred to mice that express Cre recombinase, the SK1 sequences encoding the translation initiation site through transmembrane domain five are deleted in the cre-expressing tissues of the offspring. These SK1-flox (delta neo) mutant mice may be useful in studying the role of small-conductance calcium-activated potassium (SK) channels in after-hyperpolarization and action potentia .....
For more information please see the full phenotype on the strain data sheet
006891 B6.129-Mgat1tm2Jxm/J
Cryopreserved - Ready for recovery
Mice homozygous for this floxed targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to tissue-specific cre transgenic strains, the loxP-flanked exon is deleted by cre expression.
006892 B6.129-Mgat2tm1Jxm/J
Cryopreserved - Ready for recovery
Mice homozygous for this floxed targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exon is deleted by cre expression.
006903 B6.129-Mgat4btm1Jxm/J
Cryopreserved - Ready for recovery
Mice that are homozygous for the floxed targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele.
007003 B6.129-Mycntm1Psk/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.

For example, when crossed to a strain expressing Cre recombinase in neuronal and glial cell precursors (see Stock No. 003771), this mutant mouse strain may be useful in studies of neurogenesis.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published r .....
For more information please see the full phenotype on the strain data sheet

021777 B6.129-Nrxn1tm3Sud/J
Cryopreserved - Ready for recovery
Neurexins act as cell adhesion molecules and receptors involved with synaptic function. In this conditional targeted mutation strain, the last coding exon (exon 24) of the Nrxn1 (neurexin 1) gene is flanked by loxP sites. Excision of the floxed segment through Cre recombinase expression effectively truncates the gene product. A 2xHA (hemagglutinin) tag outside of the floxed region provides a useful marker. Immunoblotting, immunohistochemistry, and immunocytochemistry confirm that the HA tag is functional.
004860 B6.129-Ogttm1Gwh/J
Cryopreserved - Ready for recovery
These mice possess loxP restriction sites on either side of the exon encoding amino acids 206-232 of the X-linked Ogt gene. Female mice bearing two copies of the floxed allele, and males carrying one, are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. At least one intact, functional Ogt allele is required for embryonic stem cell viability and subsequent ontogenic events, as well as the viability and function of germ cells, neurons, thymoyctes, fibroblasts. When used in conjunction with a Cre recombinase-expressing strain, this mutant is useful in generating tissue-specific mutants of the floxed gene.

For example, when crossed to a strain expressing Cre recombinase in thymocytes (see Stock No. 003802), this mutant mouse strain may be useful in studies of intracellular glycosylation and T cell apoptosis.

When crossed to a strain expressing Cre rec .....
For more information please see the full phenotype on the strain data sheet

008397 B6.129-Pcyt1atm1Irt/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 4 and 5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 4 and 5 deleted in the cre-expressing tissue(s).

When bred to a strain with inducible Cre recombinase expression in the myeloid cell lineage (see Stock No. 004781 for example), this mutant mouse strain may be useful in studies of atherosclerosis.

005702 B6.129-Pik3c2btm1Pkha/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 3-5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain can be used to generate tissue-specific mutants of the floxed allele.
019423 B6.129-Pkhd1tm2Cjwa/J
Cryopreserved - Ready for recovery
PKHD1 (polycystic kidney and hepatic disease 1) or fibrocystin, is a large type I membrane protein associated with human autosomal recessive polycystic kidney disease (ARPKD). Pkhd1 is highly expressed in the kidney; the protein undergoes processing and is secreted by exosome-like vesicles (ELV) in the bile and urine. Pkhd1LSL mutant mice possess a loxP-STOP-loxP (LSL) cassette inserted into intron 2 and two SV5-Pk epitope tags inserted in-frame into exon 3. The LSL cassette terminates all transcripts in intron 2 generating a null allele. Mice that are homozygous for Pkhd1LSL exhibit progressive polycystic liver disease and liver fibrosis visible by histology at one month of age. Females, but not males, develop proximal tubule dilation and cysts in the kidneys.

When crossed to mice carrying Tg(Gdf9-cre)5092Coo (Stock No. 011062), the LSL cassette is removed, the Pkhd1 gen .....
For more information please see the full phenotype on the strain data sheet

010559 B6.129-Pou4f2tm2.1Nat/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of the Pou4f2 (POU domain, class 4, transcription factor 2) coding region followed by an alkaline phosphatase (AP) reporter. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a cre recombinase-expressing strain, the offspring express AP in the retina, several brain nuclei, dorsal root ganglia, trigeminal ganglion, and other cells/tissues.
010560 B6.129-Pou4f3tm1.1Nat/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of the Pou4f3 (POU domain, class 4, transcription factor 3) coding region followed by an alkaline phosphatase (AP) reporter. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a cre recombinase-expressing strain, the offspring express AP in the retina, dorsal root ganglia, and other cells/tissues.
021159 B6.129-Ptp4a3tm1Geh/J
Cryopreserved - Ready for recovery
Ptp4a3 (protein tyrosine phosphatase 4a3) is a prenylated/lipidated protein tyrosine phosphatase that is highly expressed in many human cancers (including tumors of the colon, breast, ovary, liver, stomach, and stroma). It is correlated with tumor metastasis and poor patient prognosis, but is not well characterized.

These mice carry a floxed allele of the gene. Exon 2, encoding the transcriptional start site of the gene, is flanked by loxP sites. Excision of the floxed region with Cre recombinase results in a null allele, as determined by Western blot analysis.

Global homozygous null mice were generated from crosses with a transgenic EIIa-Cre strain (e.g. see Stock No. 003724). Cre was subsequently bred out of the knockout model. Mice deficient for functional Ptp4a3 are grossly and histologically normal, but fewer null males are reportedly observed at weaning age and they maintain a slightly (10%) de .....
For more information please see the full phenotype on the strain data sheet

005357 B6.129-Sema3ftm1.1Ddg/J
Cryopreserved - Ready for recovery
These mice possess loxP sites 4kb upstream of exon 1 and 1 kb downstream of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
When bred to a strain expressing Cre recombinase in neuronal cells (see Stock No. 003966 for example), this mutant mouse strain may be useful in neurobiology research.
018982 B6.129-Slc12a6tm1Garo/J
Cryopreserved - Ready for recovery
Slc12a6 is a member of the solute carrier family that encodes the K+/Cl- cotransporter 3 (KCC3 or SLC12A6). KCC3 has a role in ion homeostasis and is associated with the neurodevelopmental and neurodegenerative disorder called hereditary motor and sensory neuropathy associated with agenesis of the corpus callosum (HMSN/ACC). Most HMSN/ACC patients carry a deletion in exon 18 (a conserved splice donor site) that results in a stop codon at position 813 (Thr813X). KCC3 is expressed in brain, kidney, muscle lung and heart. These mutant mice possess loxP sites flanking exon 18 of the Slc12a6 gene. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express Cre recombinase, the resulting offspring will have exon 18 deleted in the cre-expressing tissues resulting in a C terminal truncation of the protein mimicking the human HMSN/ACC mutation. These mice may be useful in generating conditional knockouts fo .....
For more information please see the full phenotype on the strain data sheet
014604 B6.129-Slc17a9tm1.1Rpa/J
Cryopreserved - Ready for recovery
Mice homozygous for this Slc17a9flox allele are viable and fertile, with loxP sites flanking exons 2-3 of the solute carrier family 17, member 9 (Slc17a9) gene. Slc17a9 is is a vesicular nucleotide transporter expressed in the adrenal gland, brain, and thyroid gland. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the endogenous signal peptide deleted in the cre-expressing tissue(s); this is expected to produce a null allele. These mutant mice may be useful in generating conditional mutations for studying the role of Slc17a9 in vesicular storage and ATP exocytosis.
006146 B6.129-Smn1tm1Jme/J
Cryopreserved - Ready for recovery
Mice homozygous for this SMNF7 floxed allele are viable and fertile and do not display any gross physical or behavioral abnormalities. Mutant mice exhibit no transcript splicing defects. Cre-mediated recombination of the loxP-flanked sequences results in deletion of exon 7 of the targeted gene. As mutations of this exon are implicated in 95% of all human spinal muscular atrophy (SMA), these mice may be useful in studying SMA or other neuromuscular degenerative diseases.

When crossed to a strain expressing Cre recombinase in neurons (see Stock No. 005938, Stock No. 006297, and Stock No. 006663), this mutant mouse strain may be useful as a model of SMA.

When crossed to a strain expressing Cre recombinase in striated muscle fibers (see Stock No. For more information please see the full phenotype on the strain data sheet

008133 B6.129-Sncbtm1Sud/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, normal in size and do not display any gross physical or behavioral abnormalities, but breed very poorly. Protein levels are normal.

When bred to a strain expressing Cre recombinase, this mutant mouse strain may be useful in studies of presynaptic proteins and synaptic vesicles.

006897 B6.129-St3gal1tm2Jxm/J
Cryopreserved - Ready for recovery
Mice homozygous for this floxed targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele.
006898 B6.129-St3gal2tm2Jxm/J
Cryopreserved - Ready for recovery
Mice homozygous for the floxed targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele.
006900 B6.129-St3gal4tm1.1Jxm/J
Cryopreserved - Ready for recovery
Mice homozygous for this targeted mutation are viable, fertile, and normal in size. They develop a bleeding disorder associated with an autosomal dominant reduction in plasma von Willebrand factor (VWF) and an autosomal recessive thrombocytopenia. The formation of selectin ligands on circulating neutrophils is also substantially reduced. This mutant mouse strain may be useful in studies of leukocyte trafficking and coagulation disorders.
006901 B6.129-St6gal1tm2Jxm/J
Cryopreserved - Ready for recovery
Mice homozygous for this floxed targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exons are deleted by cre expression to produce a null allele.
008291 B6.129P2(C)-Ptprjtm1.1Weis/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 18, encoding a transmembrane domain of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain can be used to generate tissue- or cell-specific mutants of the floxed allele. These mice may be useful in studies of immune responses.
019105 B6.129P2(Cg)-Bub1tm1Tvd/J
Cryopreserved - Ready for recovery
The serine/threonine kinase encoded by Bub1 is critical to spindle-assembly checkpoint signaling and correct chromosome alignment. These mice possess loxP sites on either side of exons 2 and 3 of the Bub1, budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae), gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 and 3 deleted in the cre-expressing tissues.
022364 B6.129P2(SJL)-Ntrk3tm1Ddg/J
Cryopreserved - Ready for recovery
013224 B6.129P2-Abl1tm2.1Goff/J
Cryopreserved - Ready for recovery
These Abl1flox/flox mutant mice posses loxP sites flanking exons 5-6 of the c-abl concogene 1, non-receptor tyrosine kinase gene, Abl1. Mice that are homozygous for this allele are viable, fertile, and normal in size. When these Abl1flox/flox mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exons 5-6 deleted in the cre-expressing tissue. This strain may be useful for studying the growth, development, and physiology of cardiac tissue.
019360 B6.129P2-Acsl1tm1Rcol/J
Cryopreserved - Ready for recovery
The isozyme encoded by the acyl-CoA synthetase long-chain family member 1 (Acsl1) gene catalyzes the synthesis of acyl-CoA from fatty acids that are between 10 and 22 carbons in size. These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in the liver (see Stock No. 003574 for example), the resulting mutant mouse strain exhibits a 50% decrease in total hepatic acyl-CoA synthetase enzyme activity and may be useful in studies of lipid metabolism and liver physiology.

When bred to a strain with Cre recombinase expression in adipose tissue (see Stock No. 005069 .....
For more information please see the full phenotype on the strain data sheet

008765 B6.129P2-Cbfbtm1Itan/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 5 in the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.

When bred to CD4-cre mice, homozyogous animals have reduced peripheral T cell numbers, abnormal CD4/CD8 expression, and altered response to antigen receptor stimulation. In addition, these mice show autoimmune colitis and asthma-like syndrome.

018320 B6.129P2-Csktm1Tara/J
Cryopreserved - Ready for recovery
Csk (c-src tyrosine kinase) is a negative regulator of Src family protein tyrosine kinases (PTKs). It controls antigen receptor-mediated development and selection of T-lineage cells. Inactivation of the gene in immature thymocytes abrogates the requirement of preTCR, αβTCR and major histocompatibility complex (MHC) class II for the development of CD4+CD8+ double-positive and CD4+ single-positive thymocytes as well as peripheral CD4 αβT-lineage cells. TCR-MHC interactions have no impact on positive selection and commitment to the CD4 lineage in the absence of CSK. However, TCR-mediated negative selection of Csk-deficient, TCR transgenic cells is normal.

Exons 9 and 10 of these Csk mutant mice are flanked by loxP sites. Mice that are homozygous for this conditional allele are viable and fertile. When crossed with a cre recombinase-expressing strain, these mice are useful for creating tissue-specific kn .....
For more information please see the full phenotype on the strain data sheet

012636 B6.129P2-Cyp1a1tm1Dwn/Mmjax
Cryopreserved - Ready for recovery
Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Excision of the floxed fragment in intestines and treatment with benzopyrene (BaP) results in immunosuppression and shortened lifespan. This mutant strain is useful in organ specific studies involving aryl hydrocarbon (AHR) ligands, immunosuppression and metabolism of polycyclic aromatic hydrocarbon (PAH).
021790 B6.129P2-Cyp51tm1Bfro/J
Cryopreserved - Ready for recovery
These Cyp51flox/flox mutant mice possess loxP sites flanking exons 3-4 of the cytochrome P450, family 51 (Cyp51) gene. Mice that are homozygous for this allele are viable and fertile. CYP51 is an enzyme that catalyzes the oxidation of organic substances in metabolic processes such as cholesterol synthesis and drug metabolism. Inactivation of CYP51 is thought to give rise to a similar phenotype as mutation in the human gene POR (cytochrome P450 oxidoreductase) that has been associated with Antley-Bixler syndrome (ABS), characterized by skeletal, cardiac, and urogenital abnormalities. When these mutant mice are bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 3-4 deleted in the cre-expressing tissues.

For examples, when bred to B6.FVB-Tg(EIIa-cre)C5379Lmgd/J mice (Stock No. 003724) with widespread cre expression, resulting offspring .....
For more information please see the full phenotype on the strain data sheet

008513 B6.129P2-Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/J
Cryopreserved - Ready for recovery
A loxP-flanked neomycin cassette blocks expression of the rat Trpv1 (transient receptor potential cation channel, subfamily V, member 1) gene driven by the Gt(ROSA)26Sor gene in this targeted mutation/knock-in strain. Upon crossing to a tissue-specific Cre-expressing strain, TRPV1 and enhanced cyan fluorescent protein (ECFP) is expressed from the ROSA locus. Cells expressing TRPV1 are sensitive to capsaicin and similar chemical agonists. Treatment of mice or cells that have undergone Cre excision to remove the neomycin cassette can induce strong inward currents, trigger action potentials and activate stereotyped behaviors, allowing cell-type specific chemical genetic control of neuronal activity in vitro and in vivo. Mice that are homozygous for the floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

When bred to a strain with widespread expression of Cre recombinase (see Stock No. > .....
For more information please see the full phenotype on the strain data sheet

013586 B6.129P2-Gt(ROSA)26Sortm1Nik/J
Cryopreserved - Ready for recovery
These 3373 3-state Cre-sensitive (3373 3SCS) mice contain a construct designed to insert (from 5' to 3') a floxed-STOP cassette, tdTomato open reading frame (ORF), and a floxed-internal ribosome entry site (IRES) fused to an enhanced green fluorescent protein (GFP) ORF. The IRES-eGFP element was flanked by 3373 mutant loxP sites (which recombine at 10% the efficiency of wildtype loxP sites). This vector was inserted into the Gt(ROSA)26Sor locus allowing for widespread expression. The floxed-STOP cassette causes termination of transcription and results in no expression of either fluorophore. When bred to mice that express Cre recombinase, offspring will have the floxed-STOP cassette deleted in the cre-expressing tissue(s). This deletion results in tdTomato and/or GFP expression depending on how much Cre-recombinase the cells express. When crossed with a strain expressing low amounts of Cre recombinase, partial recombination results in tdTomato+ GFP> .....
For more information please see the full phenotype on the strain data sheet
013587 B6.129P2-Gt(ROSA)26Sortm3Nik/J
Cryopreserved - Ready for recovery
These 5172 3-state Cre-sensitive (5172 3SCS) mice contain a construct designed to insert (from 5' to 3') a floxed-STOP cassette, tdTomato open reading frame (ORF), and a floxed-internal ribosome entry site (IRES) fused to an enhanced green fluorescent protein (GFP) ORF. The IRES-eGFP element was flanked by 5172 mutant loxP sites (which recombine at 30% the efficiency of wildtype loxP sites). This vector was inserted into the Gt(ROSA)26Sor locus allowing for widespread expression. The floxed-STOP cassette causes termination of transcription and results in no expression of either fluorophore. When bred to mice that express Cre recombinase, offspring will have the floxed-STOP cassette deleted in the cre-expressing tissue(s). This deletion results in tdTomato and/or GFP expression depending on how much Cre-recombinase the cells express. When crossed with a strain expressing low amounts of Cre recombinase, partial recombination results in tdTomato+ GFP> .....
For more information please see the full phenotype on the strain data sheet
010486 B6.129P2-Isg15tm1Kpk/J
Cryopreserved - Ready for recovery
Mice homozygous for this allele are viable and fertile and possess a loxP site flanked neomycin resistance cassette disrupting exon 2 of the targeted gene. Composition of the major cell populations of the immune system in spleen, bone marrow, lymph nodes and thymus is not affected by the mutation. Homozygous mice have an increased susceptibility to influenza A/B, HSV-1 and Sinbis virus, however, response to VSV and LCMV is unaltered from control mice. These mice may be useful in studying interferon-induced antiviral responses and differing susceptibilities to viral infection.
016917 B6.129P2-Lamc1tm1Strl/J
Cryopreserved - Ready for recovery
These fLamγ1 mice possess a frt-flanked neomycin resistance (neo) cassette upstream of exon 2, and loxP sites flanking exon 2 of the laminin, gamma 1 (Lamc1) targeted gene. Homozygotes are viable, fertile, and normal in size. The donating investigator reports that the frt-flanked neo cassette that is present upstream of the floxed exon and does not appear to effect expression of Lamc1. Laminins are extracellular matrix proteins composed of heterotrimeric α, β, and γ chains. Laminins regulate basement membrane assembly, and cell proliferation, differentiation, viability, and function. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue. For example, when Lamininγ1 expression is disrupted in Schwann cells (Tg(Mpz-cre)1Mfel; see Stock No. 017927 for example), th .....
For more information please see the full phenotype on the strain data sheet
017622 B6.129P2-Pdgfbtm2Cbet/J
Cryopreserved - Ready for recovery
These PDGF-Bflox mutant mice possess loxP sites flanking exon 4 of the platelet-derived grown factor gene. Mice deficient in Pdgfb exhibit prenatal lethality, renal, cardiovascular and hematological abnormaliites. Mice that are homozygous for this conditional allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 4 deleted in the cre-expressing tissues. For example, when bred to a mouse expressing cre in the endothelium, this strain may be useful in generating conditional knockouts to study the role of pericyte recruitment and function in development and adult physiology.
017443 B6.129P2-Pdha1tm1Ptl/J
Cryopreserved - Ready for recovery
These Pdha1flox8 mutant mice possess loxP sites flanking exon 8 of the pyruvate dehydrogenase E1 alpha 1 (Pdha1) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. PDHA1 is catalytic component of the pyruvate dehydrogenase complex (PDC) which is a mitochondrial multienzyme complex involved in lipid synthesis, glucose homeostasis, and metabolism of carbohydrates. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 8 deleted in cre-expressing tissues. For example, when crossed to a strain expressing Cre recombinase in the liver, this mutant mouse strain may be useful for studying lipid metabolism, glucose metabolism, and insulin sensitivity in the liver.

When bred to a strain expressing Cre recombinase in the central and periphal nervous system (see Stock No. For more information please see the full phenotype on the strain data sheet

009154 B6.129P2-Polbtm1Rsky/J
Cryopreserved - Ready for recovery
These polβflox (or polBflox) mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissue(s). This mutant mouse strain may be useful in generating conditional mutations for studying DNA repair.
007685 B6.129P2-Psen1tm1Vln/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 7 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these "floxed" mice are bred to mice that express Cre recombinase, resulting offspring can have one of three resulting genotypes (only exon 7 deleted, only the neo selection cassette deleted, or both exon 7 and the neo selection cassette deleted) in the cre-expressing tissue(s). These PS1-floxed mice may be useful in generating conditional knockouts of Presenilin 1 for studying Alzheimer's Disease.

For example, when crossed to a strain expressing Cre recombinase in postnatal neurons (see Stock No. 006143), this mutant mouse strain may be useful in studies of amyloid plaque formation.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are .....
For more information please see the full phenotype on the strain data sheet

008336 B6.129P2-Ptpn6tm1Rsky/J
Cryopreserved - Ready for recovery
Mice homozygous for the Ptpn6f allele are viable and fertile, with loxP sites flanking exon 1(II) through most of exon 9 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in cre-expressing tissue(s). These Ptpn6f mice may be useful in generating conditional mutations for studying the role of Ptpn6 (Shp1) in inflammation and immunology research.

For example, when bred to a strain with inducible Cre recombinase expression in liver and lymphocytes (see Stock No. 003556), this mutant mouse strain may be useful in studying the motheaten (me) phenotype; characterized by widespread inflammation and autoimmunity.

When bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 004126, Stock No. > .....
For more information please see the full phenotype on the strain data sheet

008772 B6.129P2-Runx1tm1Tani/J
Cryopreserved - Ready for recovery
Exon 4 of these targeted mutant mice is flanked by loxP sites. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. When crossed to a CD4-cre mouse strain, progeny have fewer CD4+ T cells than wildtype mice and completely lack NK T cells.
008773 B6.129P2-Runx3tm1Itan/J
Cryopreserved - Ready for recovery
Exon 4 of these targeted mutant mice is flanked by loxP sites. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.

When crossed to either a CD4-cre or Lck-cre mouse strain, the numbers of CD8+ mature thymocytes and CD8+ T cells in spleen or lymph nodes are reduced and show defective responses to antigen receptor stimulation. CD8+ T cells express CD4 and the ectopic CD4 expression is enhanced when the floxed region is excised.

018419 B6.129S(Cg)-Commd1tm2.1Bvds/J
Cryopreserved - Ready for recovery
These Commd1loxP mutant mice possess loxP sites flanking exon 1 of the Commd1 (COMM domain containing 1) gene. COMMD1 plays a role in copper homeostasis, sodium transport, antioxidant defense, SOD-1 maturation, and NF-κB and hypoxia signaling. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in cre-expressing tissues. Germ-line deletion of COMMD1 results in embryonic lethality.

When bred to B6.Cg-Tg(Alb-cre)21Mgn/J mice (Stock No. 003574), COMMD1 deficiency in the liver results in a 3-fold increase in hepatic copper levels when mice are fed a normal chow, and a progressive 20-fold increase when fed a diet high in copper.

024054 B6.129S-Nr5a2tm1Sakl/DjmJ
Cryopreserved - Ready for recovery
These LRH-1flox/flox mutant mice possess loxP sites flanking exon 5, encoding the second zinc finger of the DNA-binding domain of the nuclear receptor subfamily 5, group A, member 2 (Nr5a2) gene. LRH-1 is a transcription factor expressed in liver, pancreas, intestine, and ovary where it regulates cholesterol and bile acid homeostasis, cell proliferation, and steroidogenesis. Homozygous mice are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 5 deleted in the cre-expressing tissues.

For example, when bred to B6.Cg-Tg(Alb-cre)21Mgn/J mice (Stock No. 003574) expressing cre in the liver, flox/flox:AlbCre mice contain decreased levels of circulating cholesterol and aspartate transaminase. They have an abnormal composition of bile acid.

013085 B6.129S-Pbx1tm3.1Mlc/J
Cryopreserved - Ready for recovery
These Pbx1f/f mutant mice possess loxP sites flanking exon 3 of the targeted pre B-cell leukemia transcription factor 1 (Pbx1) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues. This strain may be useful for studying the effect of Pbx1 on the maintenance and development of multiple tissue lineages.

For example, when crossed to a strain expressing interferon-inducible Cre recombinase in multiple tissues (see Stock No. 003556), this mutant mouse strain may be useful in studies of hematopoietic stem cell renewal.

When crossed to a strain expressing Cre recombinase in endothelial cells (see Stock No. For more information please see the full phenotype on the strain data sheet

018780 B6.129S-Pknox1tm1.1Xzh/J
Cryopreserved - Ready for recovery
These Prep1flox mutant mice possess loxP sites flanking exon 8 of the Pbx/knotted 1 homeobox (Pknox1) gene. PREP1 is an upstream enhancer of PAX6 signaling required for lens and pancreas development. PREP1-deficiency is implicated in the development of some spontaneous cancers. Mice that are homozygous for this allele are viable, fertile, and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, resulting offspring will have exon 8 deleted in cre-expressing tissues. PREP1 deficiency in the lens and pancreas do not result in developmental defects.
012646 B6.129S-Scpep1tm1Jomm/J
Cryopreserved - Ready for recovery
In these mice endogenous gene function of the serine carboxypeptidase 1 (Scpep1) gene is replaced with a β-galactosidase (lacZ) gene. Mice homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. SCPEP1 expression promotes smooth muscle cell proliferation and migration after injury to the vessel wall. Scpep1 KO mice exhibit a reduction in arterial injury-induced neointimal area as well as decreases in the area bound by the external elastic lamina after vessel wall injury. These mice may be useful for studying vascular remodeling after injury and vascular occlusive diseases.
008650 B6.129S-Trpa1tm2Kykw/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of the S5/S6 transmembrane domains of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, the S5/S6 transmembrane domains of the targeted gene are deleted in the tissue of interest. This strain may be useful in functional studies of cation channels.
017444 B6.129S1(Cg)-Cops8tm1Nwe/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 4-6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 4-6 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in T cells, this mutant mouse strain may be useful in studies of T cell activation and proliferation.

013542 B6.129S1(Cg)-Dnm2tm1.1Pdc/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 2 of the Dnm2 (dynamin 2) gene. Mice that are homozygous for this floxed allele are viable, fertile, and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this floxed strain is useful in eliminating expression of Dnm2 in a tissue-specific fashion (documented in cultured fibroblasts). Germline deletion results in embryonic lethality. This strain may be useful in further characterizing the role of this gene in endocytosis.
009367 B6.129S1-Bptftm1.1Cwu/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s). This mutant mouse strain may be useful in generating conditional mutations for studying chromatin remodeling during development.
023713 B6.129S1-C1galt1tm1.1Rpmc/LxJ
Cryopreserved - Ready for recovery
These T-synF mice contain loxP sites flanking exons 1-2 of the core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1 (C1galt1) gene. T-syn facilitates the transfer of galactose (Gal) from Uridine diphosphate (UDP)-Gal to GalNAcα1-Ser/Thr (Tn antigen) to form the core 1 O-glycan, Galβ1-3GalNAcα1-Ser/Thr (T antigen). Tn antigen has been associated with the development of carcinomas and some autoimmune diseases. T-syn plays a role in processes such as angiogenesis, thrombopoiesis, and kidney homeostasis development. O-glycans, are also primary components of mouse colon mucus and plays a protective role against carcinomas and some autoimmune diseases such as ulcerative colitis (UC) and Inflammatory bowel disease (IBD). Homozygotes are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 1-2 deleted in cre-expressing ti .....
For more information please see the full phenotype on the strain data sheet
008637 B6.129S1-Kif5btm1Njen/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 2 in the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene to elucidate the gene's function.

For example, when crossed to a strain expressing Cre recombinase in pancreatic beta cells (see Stock No. 003573), this mutant mouse strain may be useful in studies of beta cell development and insulin secretion.

018395 B6.129S1-Snrpntm2.1Kaj/J
Cryopreserved - Ready for recovery
Mice homozygous for this PWS-ICflox6kb allele are viable and fertile, with loxP sites flanking the 6 kb Prader-Willi Syndrome imprinting center (PWS-IC) region around exon 1 of the Snrpn gene . When bred to mice that express Cre recombinase, the resulting offspring will have this area deleted in the cre-expressing tissue(s). Because Snrpn is imprinted and only expressed from the paternal allele, breeding PWS-ICflox6kb males with cre-expressing females is required to generate deleted offspring with the knockout phenotype. These PWS-ICflox6kb mice be useful in studying the temporal and spatial functions of the Prader-Willi imprinting center.

When crossed to female mice carrying the Tg(CMV-cre)1Cgn allele (see Stock No. 006054), widespread Cre recombinase expression prior to implantation results in postnatal lethality.

When crossed to female mice carrying the T .....
For more information please see the full phenotype on the strain data sheet

017642 B6.129S1-Is(14)2Rdf/J
Cryopreserved - Ready for recovery
In B cell chronic lymphocytic leukemia the most common genomic aberration is deletion of human chromosomal region 13q14, corresponding to mouse chromosomal region 14qC3. Deletions of this same region are also found in monoclonal B-cell lymphocytosis and diffuse large B-cell lymphomas. An 0.11Mb minimal deleted region (MDR), that includes DLEU2 (deleted in lymphocytic leukemia, 2) and the MIR15A/MIR16-1 cluster, has been identified in more than half the patients with B cell chronic lymphocytic leukemia. These mice carry the conditional (floxed) MDR (minimal deleted region) allele, 14qC3-MDR. loxP and FRT sites flank a 110 kb region that includes the Dleu5, Kcnrg, Dleu2 genes and Mir15a/Mir16-1 cluster. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cre-mediated recombination is indicated by GFP expression.

When bred to a s .....
For more information please see the full phenotype on the strain data sheet

015828 B6.129S2(FVB)-Pak4tm2.1Amin/J
Cryopreserved - Ready for recovery
These Pak4 floxed mutant mice possess loxP sites flanking exons 2-4 of the p21 protein (Cdc42/Rac)-activated kinase 4 (Pak4) gene. Homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Pak4 is a member of the group B family of PAK serine/threonine kinases and is expressed early in development in a variety of tissues. It is involved in the formation of filopodia in response to Cdc42, promoting neuronal growth. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2-4 deleted in cre-expressing tissues. This strain may be useful for studying the role of Pak4 in the development of extraembryonic tissue, embryonic vasculature, and the placenta.
016937 B6.129S2-Lattm6(DTR)Mal/J
Cryopreserved - Ready for recovery
Latfl-dtr mutant mice contain a loxP site downstream from exon 1, and an internal ribosome entry site (IRES), a human diphtheria toxin receptor, and an enhanced green fluorescent protein (EGFP) followed by a second loxP site downstream of the internal stop codon of the linker for activation of T cells (Lat) gene. Homozygotes are viable, fertile, and normal in size. LAT is a transmembrane protein expressed in natural killer (NK) cells, mast cells, platelets, megakaryocytes, and T lymphocytes of the thymus and peripheral lymphoid tissues. Following T cell receptor (TCR) activation, LAT phosphorylation triggers downstream T cell-specific signaling pathways. These mice express 1.2-fold less LAT than control mice, with no apparent effect on T cell function. Diphtheria toxin (DT) administration results in acute ablation of LAT-expressing T thymocytes in the thymus and peripheral lymphoid tissues. When these mutant mice are bred to mice that expr .....
For more information please see the full phenotype on the strain data sheet
014089 B6.129S2-Rbfox1tm1.1Dblk/J
Cryopreserved - Ready for recovery
These Fox1flox mutant mice possess loxP sites flanking exons 11-12 of the RNA binding protein, fox-1 homolog 1 (Rbfox1) gene. Mice that are homozygous for this allele are viable, fertile, and normal in size. Fox-1 is expressed in brain, heart, and skeletal muscle and regulates alternative splicing in vertebrates by binding specifically to (U)GCAUG sequences. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exons 11-12 deleted in the Cre-expressing tissue, resulting in inactivation of Fox-1 gene function.

For example, when crossed to a strain expressing Cre recombinase in the central and peripheral nervous system (see Stock No. 003771), this mutant mouse strain may be useful in studies of neuronal excitation and seizures.

When bred to mice carrying Tg(Pcp2-cre)2Mpin (Stock No. For more information please see the full phenotype on the strain data sheet

014156 B6.129S4(Cg)-Cdk5tm1.1Lht/J
Cryopreserved - Ready for recovery
These Cdk5 floxed mutant mice possess loxP sites flanking exons 1-5. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, resulting offspring lack detectable Cdk5 in the cre-expressing tissues.
017322 B6.129S4(Cg)-Dgat1tm2Far/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 14 through 17 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 14 through 17 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in skin (see Stock No. 004782 for example), this mutant mouse strain may be useful in studies of retinoid homeostasis.

006490 B6.129S4-Abcb7tm1Mdf/J
Cryopreserved - Ready for recovery
Homozygous mice are viable and fertile with no reported neurological or hematological abnormalities. These mutant mice have loxP sites flanking exons 9 and 10 of the endogenous gene. When bred to Cre recombinase expressing mice, exons 9 and 10 are deleted in the offspring dependent on the tissue specificity of the Cre recombinase expressing parent. The donating investigator reports that the null allele is not transmissible due to an effect on the extraembryonic tissues. This mutant may be useful in studying cytosolic Fe-S cluster assembly and metabolism, Friedreich ataxia, anemia, and hematopoiesis.

When bred to a strain expressing Cre recombinase in liver (see Stock No. 003574 for example), this mutant mouse strain may be useful in studies of hepatocyte iron metabolism.

When bred to a strain expressing Cre recombinase in epiblast derived cells (see Stock No. For more information please see the full phenotype on the strain data sheet

024286 B6.129S4-Abl1tm1Ajk/J
Cryopreserved - Ready for recovery
The Abl1 protooncogene encodes for a nonreceptor tyrosine kinase which is involved processes such as signal transduction in cytoskeleton reorganization, stress response, cell adhesion and differentiation, apoptosis, and phagocytosis. These mice possess loxP sites on either side of exon 5 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 5 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in neuronal lineage cells, this mutant mouse strain may be useful in studies of dendrite branching.

010490 B6.129S4-Clocktm1Rep/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 5 and 6 of the targeted gene. Mice that are homozygous for this conditional allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 5 and 6 deleted in the cre-expressing tissue(s).

When bred to a strain expressing Cre recombinase in the male germ line (see Stock No. 003328 for example), this mutant mouse strain may be useful in studies of circadian rhythm and behavior.

010487 B6.129S4-Csnk1dtm1Drw/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this conditional allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues. After Cre-mediated excision of exon 2, a mutant gene product (protein) is detected by Western blot analysis of liver tissue. This mutant protein is relatively unstable, does not interact with PER (Period) proteins, and does not have biological activity with respect to PER protein phosphorylation. This mutant mouse strain may be useful in generating conditional mutations for studying circadian rhythm and behavior.
010489 B6.129S4-Csnk1etm1Drw/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this conditional allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 and 3 deleted in the cre-expressing tissue(s). This mutant mouse strain may be useful in generating conditional mutations for studying circadian rhythm and behavior.
007671 B6.129S4-Fgfr1tm5.1Sor/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 4 of the targeted gene. Exon 4 is the first exon found in all reported Fgfr1 splice variants. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 and the neomycin selection cassette deleted in the cre-expressing tissue(s). This mutant mouse strain may be useful in studies of fibroblast growth factor (Fgf) cellular signaling during embryonic development.
010960 B6.129S4-Grk5tm2Rjl/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 7 and 8 of Grk5 (G protein-coupled receptor kinase 5). Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene, exons 7 and 8 of the targeted gene are deleted in the cre-expressing tissues in the offspring mice. The floxed allele appears to be fully functional and expresses normal levels of GRK5 protein.
010962 B6.129S4-Grk6tm1Mca/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 3-9 of Grk6 (G protein-coupled receptor kinase 6). Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene, exons 3-9 of the targeted gene are deleted in the cre-expressing tissues in the offspring mice. The floxed allele appears to be fully functional and expresses normal levels of GRK6 protein.
021210 B6.129S4-Grntm1Far/Mmjax
Cryopreserved - Ready for recovery
These Grnflox mutant mice possess loxP sites flanking exons 2-13, which comprises the coding sequence and the 3'UTR of the granulin precursor (or progranulin) gene. Progranulin is a glycopeptide secreted by many cell types and is processed into growth modulating peptides called granulins. The human neurodegenerative syndrome frontotemporal lobar dementia (FTLD) is associated with mutations in progranulin. Progranulin also is associated with wound healing, inflammation, embryogenesis and tumor growth. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have a null allele in the cre-expressing tissues.

For example, when bred to mice carrying Tg(ACTB-cre)2Mrt,widespread Cre recombinase expression results in macrophages that exhibit increased levels of phagocystosis in culture and when treated with MPTP Grn- mice exhibit an increas .....
For more information please see the full phenotype on the strain data sheet

009044 B6.129S4-Gt(ROSA)26Sortm3(CAG-luc)Tyj/J
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In the absence of Cre, there is low level expression of Luciferase-SIY H2b antigen expression. After Cre activity, the recombined locus has high expression of Luciferase-SIY fusion protein. This mutant mouse strain may be useful in studies of immune response to a self-antigen or over-expressed tumor-associated antigen when used in combination with tumor-prone models. This mouse is also useful as a reporter for Cre activity.
006503 B6.129S4-Lpltm1Ijg/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. These mice may be useful for cardiovascular studies (such as lipid metabolism and fat storage) and obesity research.

For example, when crossed to a strain expressing Cre recombinase in cardiac muscle cells (see Stock No. 011038), this mutant mouse strain may be useful in studies of cardiac lipid metabolism.

003541 B6.129S4-Ntf3tm2Jae/J
Cryopreserved - Ready for recovery
This strain has loxP sites located on either side of the Ntf3 exon 2. Mice homozygous for this allele are viable, fertile and without behavioral abnormalities. If this strain is crossed with a transgenic strain bearing Cre recombinase, expression of the Ntf3 gene in offspring can be blocked in a tissue-specific manner depending on the promoter controlling the recombinase. Strains under the control of the rat nestin promoter block expression in the central nervous system; see strains 002275 and 002276.

When bred to a strain expressing Cre recombinase in skeletal muscle (see Stock No. 007893 for example), this mutant mouse strain may be useful in studies of muscle spindle development.

When bred to mice carrying Tg(Tagln-cre)1Her (Stock No. For more information please see the full phenotype on the strain data sheet

016132 B6.129S4-Perptm2Att/J
Cryopreserved - Ready for recovery
These Perpfl/fl mutant mice possess loxP sites flanking exons 2-3 of the PERP, TP53 apoptosis effector (Perp) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. PERP is involved in maintaining epithelial integrity by promoting cell-cell adhesion in the skin and other stratified epithelia. When these mutant mice are bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 2-3 deleted in the cre-expressing tissues. When bred to mice expressing cre driven by tamoxifen-inducible Keratin 14 (Krt14), the lack of PERP expression in the epidermis, in conjunction with UVB exposure, leads to increased tumorigenesis. This strain may be useful for studying desmosomal adhesion function in stratified epithelia as well as tumor progression and suppression.
010671 B6.129S4-Pkd1tm2Ggg/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 2 through 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 4 deleted in the cre-expressing tissue(s).

For example, when crossed to a strain with widespread Cre recombinase expression (see Stock No. 003553), this mutant mouse strain may be useful in studies of polycystic kidneys.

005897 B6.129S4-Ppardtm1Rev/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 4 of the targeted gene is deleted in the tissue of interest, leading to premature termination of the translation product upstream of the DNA binding domain. This strain may be useful in generating tissue-specific mutants of the floxed allele for use in studies including embryo development, adipocyte physiology, fat metabolism and storage, inflammation, and cancer.
004825 B6.129S4-Psen1tm2Shn/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. Northern blot and RT-PCR analysis of brain tissue from homozygotes reveal gene product (mRNA) transcription levels and size are the same as wildtype. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 and 3 deleted in the cre-expressing tissues.
007583 B6.129S4-Slc17a6tm1Rpa/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 2 in the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. They show ~30% less expression of the targeted gene than wildtype mice, however. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in eliminating expression in a tissue-specific manner (widespread deletion of expression is lethal).
017603 B6.129S6(129S4)-Tfpitm1.1Rdsi/J
Cryopreserved - Ready for recovery
The TFPIFlox allele has loxP sites flanking exon 4 of the tissue factor pathway inhibitor gene [Tfpi]. Homozygous TFPIFlox mice are viable and fertile with no observed abnormalities. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the Kunitz 1 domain of TFPI protein (TFPI-K1) deleted in the cre-expressing tissues. This is designed to result in a K1-deleted form of TFPI containing the remainder of the TFPI protein (and to affect all K1-containing isoforms including TFPIα, TFPIβ, and TFPIγ).

For example, when TFPIFlox mice are bred to a strain expressing Cre recombinase in embryonic tissues (CMV-Cre; see Stock No. 006054), the resulting mice with pan deletion of the TFPI-K1 domain exhibit complete prenatal lethality; this is similar to mice homozygous for the TFPI knockout allele.

In additio .....
For more information please see the full phenotype on the strain data sheet

009673 B6.129S6(C)-Gt(ROSA)26Sortm3(HIF1A*)Kael/J
Cryopreserved - Ready for recovery
These LSL-HIF1dPA mice conditionally express a form of hemagglutinin (HA)-tagged human HIF1A (HIF1&alpha, HIF1dPA) cDNA that escapes recognition by the von Hippel-Lindau tumor suppressor protein by virtue of proline to alanine substitutions. When crossed with a tissue-specific cre strain, excision of a floxed stop cassette enables expression of the cDNA driven by the endogenous mouse Gt(ROSA)26Sor promoter. There is no expression until the mice are exposed to Cre recombinase. This strain may be useful in studies of von Hippel-Lindau disease mechanisms.

For example, when crossed to a strain expressing Cre recombinase in liver (see Stock No. 003574), this mutant mouse strain may be useful in studies of VHL disease.

006447 B6.129S6(CBA)-Cebpatm1Dgt/J
Cryopreserved - Ready for recovery
Mice carrying this C/EBPalpha "floxed" allele (C/EBPalphaF) are viable and fertile. The floxed allele functions similarly to the wildtype allele. In mice homozygous for C/EBPalphaF and expressing an interferon-inducible Cre recombinase (introduced by breeding to a cre-expressing strain; see Stock No. 003556), C/EBPalpha activity is disrupted, leading to defective myeloid cell development, increased hematopoietic stem cell repopulating activity, and significantly increased myeloblast population in the bone marrow compartment. In combination with an appropriate Cre transgenic strain, these mutant mice may be useful in studies of hematopoietic cell (e.g. myeloid and basophil progenitor cell) development and function, cancer (e.g. acute myeloid leukemia), and alveolar cell differentiation.
018782 B6.129S6(Cg)-Actl6atm1.1Grc/J
Cryopreserved - Ready for recovery
018540 B6.129S6(Cg)-Bpnt1tm2.1Yrk/J
Cryopreserved - Ready for recovery
Bpnt1 is (bisphosphate 3'-nucleotidase 1) is a magnesium-dependent phosphomonoesterase that can be inhibited by lithium, a drug used to treat manic depression.

Exons 4 and 5 are flanked by loxP sites in these Bpnt1 conditionally targeted mutation mice. The floxed mice do not show any over phenotype and expression of BPNT1 is normal with no detectable defects in liver, kidney, brain and small intestine. When the floxed segment is excised by Cre recombinase, tissue-specific knockouts of the gene can be produced.

019083 B6.129S6(Cg)-Kcnn3tm2.1Jpad/J
Cryopreserved - Ready for recovery
These floxed-SK3 mutant mice possess loxP sites flanking the translation initiation codon, coding sequences of exon 1, and a portion of intron1 of the potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3 (Kcnn3) gene. SK3 is expressed in the soma and dendrites of dopaminergic neurons in the substantia nigra and influences their action potential frequency. Defects in dopamine (DA) releasing neurons have been sighted in pathologies such as schizophrenia and Parkinson's disease. SK3 is also expressed in smooth muscle of the bladder and uterus, and endothelia of the vasculature where the channels participate in blood pressure regulation. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 1 deleted in cre-expressing tissues.
008572 B6.129S6(Cg)-Kdm5atm1Kael/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 5 and 6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding exons 5 and 6) deleted in the cre-expressing tissue(s). This strain may be useful in mechanistic studies of transcriptional regulation.

For example, when crossed to a strain expressing tamoxifen-inducible Cre recombinase in most tissues (see Stock No. 004682), this mutant mouse strain may be useful in studies of tumorigenesis.

017982 B6.129S6-Adamts18tm1Zdl/J
Cryopreserved - Ready for recovery
These Adamts18loxP/loxP mutant mice contain a frt-flanked neo cassette upstream of exon5 and possess loxP sites flanking exons 5-6 of the targeted gene. ADAMTS18 is a protease that functions as a tumor suppressor. Mice that are homozygous for this allele are viable and normal in size. The donating investigator reports some fertility defects. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 5-6, as well as the neo cassette, deleted in the cre-expressing tissues.
021145 B6.129S6-Bin1tm2Gcp/J
Cryopreserved - Ready for recovery
These Bin1flox mice possess loxP sites flanking exon 3 of the bridging integrator 1 (Bin1) gene. BIN1 is a nucleocytoplasmic adaptor protein, involved in phagocytosis, apoptosis, and synaptic vesicle endocytosis. BIN1 has been implicated in the development of cardiac defects and Alzheimer's disease and has been shown to attenuate many types of cancer. Homozygotes are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in cre-expressing tissues. Widespread deletion results in perinatal lethality due to ventricular cardiomyopathy.

When crossed to B6129-Tg(Wap-cre)11738Mam/J mice (Stock No. 003552) expressing Cre recombinase in mammary gland tissues, no long term increase in breast cancer incidence was observed in either virgin or parous animals, however, initiation with the Ras-activating carcinogen DMBA .....
For more information please see the full phenotype on the strain data sheet

015840 B6.129S6-Itga8tm1.1Rdav/J
Cryopreserved - Ready for recovery
Mice homozygous for this f(α8) conditional allele are viable and fertile, with loxP sites flanking the last two coding exons (exons 29-30) of the Itga8 gene (also called integrin alpha 8, alpha8-integrin, or α8-integrin). When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the transmembrane and the cytoplasmic domains deleted in the cre-expressing tissue(s). These f(α8) mutant mice may be useful in generating conditional mutations for studying the role of Itga8 transmembrane cell adhesion receptors in neuronal function in the developing and adult central nervous system, including intracellular signaling, behavior, synaptic plasticity, and memory formation.

For example, when f(α8) mice are bred to mice expressing Cre recombinase in forebrain neurons (see Stock No. 005359 for example), the double mutant offspring may exhibit impa .....
For more information please see the full phenotype on the strain data sheet

011123 B6.129S6-Myctm2Fwa/Mmjax
Cryopreserved - Ready for recovery
Homozygote: Mice homozygous for the targeted mutation are viable and fertile and exhibit no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific conditional mutants of the floxed gene.

Heterozygote: Not evaluated

For example, when bred to mice carrying Cd19tm1(cre)Cgn, which expresses Cre recombinase in B lymphoctye development, this mutant mouse strain may be useful in studies of B cell proliferation and activation.

When bred to mice carrying Tg(Wnt1-cre)11Rth, which expresses Cre recombinase in the neural tube, this mutant mouse strain may be useful in studies of craniofacial development.

012808 B6.129S6-Myo1etm1Flv/J
Cryopreserved - Ready for recovery
Exon 4 is flanked by loxP sites in this Myo1e (myosin IE) targeted mutation strain. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene in studies of kidney structure, function, and disease.

For example, when bred to B6.Cg-Tg(NPHS2-cre)295Lbh/J mice (Stock No. 008205), mesenchyme-specific cre-expression results in mice exhibiting albuminuria, podocyte foot process effacement, and glomerular basement membrane disorganization.

017576 B6.129S6-Psip1tm1Eng/J
Cryopreserved - Ready for recovery
These mutant mice possess loxP sites flanking a Neo cassette and exon 3 of the PC4 and SFRS1 interacting protein 1 (Psip1) gene. Psip1 encodes LEDGF or p75, a chromatin-associated protein that acts as a cofactor of lentiviral DNA integration and plays a role in determining the distribution of lentiviral integration sites. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 3 deleted in the cre-expressing tissue, resulting in inactivation of Psip1 gene function. For example, when bred to mice expressing Cre-recombinase in epiblast cells (see Stock No. 008454 for example), the cells from the resulting offspring are defective in human immunodeficiency virus type 1 (HIV-1) integration. This strain ma .....
For more information please see the full phenotype on the strain data sheet
012592 B6.129S6-Ptpn11tm1Toa/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are not viable. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in generating conditional mutations to study cardiac defects, fatal myeloproliferative disorder (MPD) and juvenile myelomonocytic leukemia (JMML).

When bred to a strain expressing Cre recombinase in endothelial cells (Tg(Tek-cre)12Flv), this mutant mouse strain may be useful in studies of the cardiac defects found in Noonan syndrome.

When bred to a strain expressing Cre recombinase in epiblast-derived tissues (Meox2tm1(cre)Sor), this mutant mouse strain may be useful in studies of cardiac defects.

When bred to a strain expressing tamoxifen-inducible Cre recombinase in most tissues (Tg(CAG-cre/Esr1*)5Amc), this mutant mouse strain may be useful in studies of hematopoiesis.

When bred to a st .....
For more information please see the full phenotype on the strain data sheet

020086 B6.129S6-Slc3a2tm1.1Merl/J
Cryopreserved - Ready for recovery
The transmembrane protein encoded by Slc3a2 (solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2) is part of a heterodimer that is involved in the transport of large neutral amino acids. These mice possess loxP sites on either side of exon 2 of the Slc3a2 gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in intestinal villi and crypt cells (see Stock No. 004586 for example), this mutant mouse strain may be useful in studies of inflammatory bowel disease (IBD) and colitis-associated cancer.

006658 B6.129S6-Srftm1Rmn/J
Cryopreserved - Ready for recovery
These mice possess loxP sites that flank promoter and exon 1 sequences. Mice that are homozygous for this allele are viable and fertile. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Excision of the floxed fragment results in the removal of key regulatory elements and many of the DNA-binding and dimerization residues of the gene.

When bred to a strain expressing Cre recombinase under the control of mouse transgelin promoter (see Stock No. 004746 for example), this mutant mouse strain may be useful in studies of cardiovascular disease.

012900 B6.129S6-Stat5a/Stat5btm2Mam/Mmjax
Cryopreserved - Ready for recovery
Homozygote: Mice homozygous for the targeted mutation are viable and fertile and exhibit no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific conditional mutants of the floxed gene. This mutant mouse strain may be useful in generating conditional mutations to study cell proliferation, differentiation and survival in in any given cell type.

Heterozygote: Not evaluated

018439 B6.129S6-Tg(CAG-Bgeo,-SMN2)E9Dscd/J
Cryopreserved - Ready for recovery
These Tg hSMN2 E9 inducible SMN transgenic mice have a loxP-flanked β-geo STOP cassette preventing transcription of a downstream full-length human SMN2 cDNA sequence (exons 1-8). Prior to Cre recombinase exposure, β-galactosidase (lacZ) expression is directed to widespread tissues by the CMV-IE enhancer/chicken β-actin/rabbit β-globin hybrid promoter, and no human SMN2 expression is observed. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed β-geo STOP cassette deleted in the cre-expressing tissues; resulting in expression of full-length human SMN2 in cre-expressing tissues (and continued lacZ expression in other tissues). The donating investigator reports that Tg hSMN2 E9 inducible SMN transgenic mice do not express human SMN2 prior to introduction of Cre recombinase. Mice hemizygous for the inducible SMN transgene are viable and fertile, with no reported phenotypic abnormalities. T .....
For more information please see the full phenotype on the strain data sheet
012565 B6.129S7(129S4)-Ift20tm1.1Gjp/J
Cryopreserved - Ready for recovery
These Ift20flox mice harbor loxP sites flanking exons 2-3 (encoding the first 71 codons including the start codon) of the intraflagellar transport 20 homolog (Chlamydomonas) locus. The primary cilium is a microtubule-based antenna-like structure that emanates from the surface of virtually all cells in the mammalian body. The primary cilium functions as a sensory organelle (mechano-, chemo-, photo-receptor) that receives signals from other cells/the environment, and transmits these signals to the nucleus to elicit a cellular response. Most types of eukaryotic cilia and flagella are assembled and maintained by the process of intraflagellar transport (IFT). During IFT, large protein complexes (IFT particles) are transported along the ciliary microtubules under the ciliary membrane. IFT particle proteins organize into at least three distinct complexes called complex A, complex B and the Golgi IFT complex. The unique role of Ift20 in both complex B as well as the Golgi IFT .....
For more information please see the full phenotype on the strain data sheet
018830 B6.129S7-Asltm1Brle/J
Cryopreserved - Ready for recovery
Asl (argininosuccinate lyase) encodes a key enzyme in the urea cycle that catalyzes the breakdown of argininosuccinate to dicarboxylic acid fumarate and the amino acid arginine, a component of the citrulline-nitric oxide (Cit-NO)cycle. Humans with ASL deficiency develop Argininosuccinic aciduria (ASA), characterized by a persistent intellectual impairment, delayed motor skills and progressive hepatic disease.

This targeted mutation strain carries a FRT-flanked neomycin cassette in intron 9 that decreases expression and activity of the gene. These hypomorphic mice are reported to express 15-20% normal levels of protein. Homozygous mice die at 3-4 weeks of age. They show severe postnatal growth retardation, abnormal hair patterning, multiple organ system dysfunction, hyperammonemia, and nitric oxide deficiency. Liver transaminase levels are elevated in plasma, renal creatinine clearance is decreased, and systolic/diastolic blood pressures are elevated compared to wild-type .....
For more information please see the full phenotype on the strain data sheet

006042 B6.129S7-Efnb2tm2And/J
Cryopreserved - Ready for recovery
Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 1 of the targeted gene is deleted in the tissue of interest. These mutant mice may be useful in studying the cellular and molecular mechanisms underlying vasculogenesis and angiogenesis, the topography of neovascularization, and adult neovascularization, including tumor angiogenesis.

To test the effectiveness of this model, these mutant mice were bred to an endothelial-specific Cre-expressing transgenic mice, Tg(Tek-cre)12Flv (Stock No. 004128) . Offspring homozygous for the Cre-mediated exon 1 deletion show angiogenic remodeling defects and embryonic death identical to homozygous Efnb2tm1And mice (see Stock No. For more information please see the full phenotype on the strain data sheet

008818 B6.129S7-Itga3tm1Rdav/J
Cryopreserved - Ready for recovery
Mice homozygous for this f(α3) conditional allele are viable and fertile, with loxP sites flanking exons 11-18 of the Itga3 (integrin alpha 3 (or alpha3-integrin (α3-integrin)) gene. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed region deleted in the cre-expressing tissue(s): such deletion leads to a non-sense mutation from direct splicing of exon 10 to exon 19 and results in a truncated peptide that is predicted to be missing more than half of the wild-type sequence, including those that encode the transmembrane and the cytoplasmic domains. These f(α3) mutant mice may be useful in generating conditional mutations for studying the role of Itga3 transmembrane cell adhesion receptors in neuronal functions in the developing and adult central nervous system, including synaptic plasticity and memory formation.

When bred to a strain expressing Cre recombinase in the hippocampal CA1 pyramidal cells .....
For more information please see the full phenotype on the strain data sheet

007579 B6.129X1(Cg)-Fgfr2tm1Dor/J
Cryopreserved - Ready for recovery
Mice homozygous for this Fgfr2flox allele possess loxP sites flanking exons 8-10 of the targeted gene and are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have sequences encoding the alternatively spliced Ig domain IIIb, as well as the IIIc and TM domains, deleted in the cre-expressing tissue(s). These Fgfr2-flox mutant mice may be useful in generating conditional mutations to study the role of fibroblast growth factor receptors in vertebrate development; including early embryogenesis, regional specification of the brain, limb morphogenesis, and normal bone, craniofacial, and lens development.

For example, when crossed to a strain expressing Cre recombinase in the central nervous system, especially astrocytes (see Stock No. 004600), this mutant mouse strain may be useful in studies of astroglial migration.

When crossed to .....
For more information please see the full phenotype on the strain data sheet

017292 B6.129X1(Cg)-Pkd2tm1.1Tjwt/J
Cryopreserved - Ready for recovery
These Pkd2cond mutant mice possess loxP sites flanking exons 11-13 of the polycystic kidney disease 2 (Pkd2) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. PKD2 is a calcium-permeable membrane channel expressed in fetal kidneys and in most adult tissues. Along with PKD1, PKD2 regulates cell proliferation, cell migration, and interactions with other cells, and is necessary for normal development and function of the kidneys. Mutations in PKD2 are responsible for 15% of all cases of autosomal dominant polycystic kidney disease (ADPKD). When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 11-13 deleted in the cre-expressing tissues. This strain may be useful for studying renal development in ADPKD.

For example, when crossed to a strain expressing Cre recombinase in endothelial cells (see Stoc .....
For more information please see the full phenotype on the strain data sheet

007236 B6.129X1(FVB)-Adamts13tm2Dgi/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homoozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.
004665 B6.129X1(FVB)-Hnf4atm1.1Gonz/J
Cryopreserved - Ready for recovery
Mice homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Similarly oriented loxP sites are positioned in introns 3 and 5 of the targeted gene. When used in conjunction with a Cre recombinase-expressing strain, this mouse can be used to generate tissue-specific mutants. Cre-directed recombination results in the deletion of the loxP-flanked sequence causing a splicing event between exons 3 and exon 6. A frame shift occurs generating a premature stop codon. A putative truncated protein may be translated, but such a protein would lack the A and T box motifs necessary for high-affinity binding to DNA. This mouse mutant may be useful in studies related to lipid homeostasis.

When bred to a strain expressing Cre recombinase in pancreatic beta cells (see Stock No. 003573 for example), this mutant mouse strain may be useful in studies of insulin .....
For more information please see the full phenotype on the strain data sheet

021983 B6.Cg-Acdtm1.1Cek/J
Cryopreserved - Ready for recovery
These Tpp1flox mice possess loxP sites flanking exons 3-8 of the adrenocortical dysplasia (Acd) gene. Acd encodes a protein that is part of the telosome/shelterin telomeric complex which is involved in telomere maintenance and protection. Mice that are heterozygous for this allele are viable and fertile. The fertility of homozygotes has not been tested, but they are smaller and are not as vigorous as heterozygotes. When these mutant mice are bred to mice that express Cre, homozygous embryos die by E8.5 due to telomere dysfunction.
006230 B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J
Cryopreserved - Ready for recovery
Mice homozygous for this C/EBPalpha "floxed" allele (C/EBPalphaF) and hemizygous for the Mx1-cre transgene are viable and fertile, and exhibit no abnormalities in the hematopoietic system. In the absence of cre expression, the C/EBPalphaF allele functions similarly to the wildtype allele. Mx1-Cre transgene expression can be induced by administration of either interferon (alpha or beta) or synthetic double-stranded RNA (such as poly I:C), leading to deletion of the "floxed" gene. Following 3-4.5 weeks of poly I:C treatment, deletion efficiency is greater than 95% in hematopoietic tissues, and C/EBPalpha protein is undetectable in bone marrow. These poly I:C-treated, mice have defective myeloid cell development, increased hematopoietic stem cell repopulating activity, and a significantly increased myeloblast population in the bone marrow compartment. These mutant mice may be useful in studies of hematopoietic cell (e.g. myeloid and basophil progenitor cell) d .....
For more information please see the full phenotype on the strain data sheet
016182 B6.Cg-Clocktm1Jt/J
Cryopreserved - Ready for recovery
Homozygous mice carrying this loxP-flanked stop-tetO cassette inserted into Clock (circadian locomotor output cycles kaput) lack expression of the targeted gene. Although untested, it is anticipated that expression can be rescued and controlled in a tissue specific manner when the mutant mice are bred with another transgenic mouse expressing tetracycline-controlled transactivator protein (tTA) under the regulation of tissue-specific promoters. Clock gene expression could be conditionally turned off in the appropriate tissue with the tetracycline analog, doxycycline. Clock expression could also be restored when the mutant mice are bred with another transgenic mouse expressing Cre to excise the loxP-flanked insert, either in the whole animal or in a tissue-specific manner.
010771 B6.Cg-Col1a1tm1(CAG-Sirt1)Dsin/Mmjax
Cryopreserved - Ready for recovery
Mice homozygous for this SIRT1STOP (or SIRT1-Tg flox-STOP-flox) targeted allele are viable and fertile, with a floxed-STOP cassette and mouse sirtuin 1 (Sirt1) cDNA sequence inserted into the 3' UTR of the collagen A1 (Col1a1) locus. When bred to mice that express Cre recombinase, offspring will have the floxed-STOP cassette deleted in the cre-expressing tissue(s), resulting in Sirt1 overexpression in Col1a1-expressing cells. These SIRT1STOP (or SIRT1-Tg flox-STOP-flox) mice may be useful for generating conditional mutations for studying the role of sirtuin NAD+-deacetylases in aging, cancer, cellular stress resistance, genomic stability, IGF1 pathways, autoimmunity, inflammation, T cell tolerance, energy metabolism, neurodegeneration, insulin resistance, and diabetes.
007920 B6.Cg-Gt(ROSA)26Sortm2(CAG-EYFP)Hze/J
Cryopreserved - Ready for recovery
Ai2 mice hemizygous for this Rosa-CAG-LSL-EYFP conditional allele are viable and fertile. A loxP-flanked STOP cassette prevents transcription of the downstream enhanced yellow fluorescent protein (EYFP). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s); resulting in expression of EYFP. Because this CAG promoter driven reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, EYFP expression is determined by which tissue(s) express Cre recombinase. The donating investigator reports that Ai2 mice do not express EYFP prior to introduction of Cre recombinase and EYFP fluorescence following exposure to cre is weak but easily detected by mRNA (in situ hybridization) and antibody staining (immunohistochemistry). Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-mediated .....
For more information please see the full phenotype on the strain data sheet
007942 B6.Cg-Isl2tm1Arbr/J
Cryopreserved - Ready for recovery
Mice homozygous for the Isl2DTA targeted mutation are viable and fertile. These mice have the diphtheria toxin (DTA) gene inserted into the Isl2 (insulin related protein 2 (islet 2)) locus. Expression of DTA in Isl2-expressing cells is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTA expression and subsequent cell ablation.

When bred to a strain expressing Cre recombinase in motor neurons (see Stock No. 006600 for example), this mutant mouse strain may be useful in neurodevelopmental studies.

These Isl2DTA mutant mice are available on a STOCK genetic background (Stock No. 007603), as well as a C57BL/6J-backcrossed background (Stock No. 007942 .....
For more information please see the full phenotype on the strain data sheet

018921 B6.Cg-Itga2tm1.1Tkun/J
Cryopreserved - Ready for recovery
Integrin alpha 2 is a receptor for collagens, laminin, decorin and collagen related-peptides, and is involved in platelet and cell adhesion to the extracellular matrix. These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable & fertile. When these mutant mice are bred to others that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in megakaryocytes (see Stock No. 008535 for example), this mutant mouse strain may be useful in studies of platelet function.

024176 B6.Cg-Mapkapk2tm1.1Yaff/J
Cryopreserved - Ready for recovery
The MK2CV allele has exon 2 of the MAP kinase-activated protein kinase 2 gene flanked with inward-facing loxP sites (loxP sites in opposing orientations). Homozygous mice are viable and fertile. Prior to Cre recombinase exposure, exon 2 is in the wildtype configuration (MK2+CV); resulting in normal MK2-expression. A single exposure to Cre recombinase creates the inverted exon 2 configuration (MK2-CV); the resulting transcript has a STOP codon in the beginning of exon 3 that encodes a highly truncated reading frame that is not translated into functional MK2 proteins (i.e., loss of both the 46 KDa and 42 KDa isoforms corresponding to Uniprot P49137-1 and P49137-2 for human MK2). Because inward-facing loxP sequences result in cre-mediated inversion rather than excision, exon 2 can reversibly switch from the MK2-expressing orientation (MK2+CV) to the MK2-null orientation (MK2-CV) and back as long as .....
For more information please see the full phenotype on the strain data sheet
016231 B6.Cg-Msh2tm2.1Rak/J
Cryopreserved - Ready for recovery
The Msh2LoxP allele has loxP sites flanking exon 12 of the mutS homolog 2 (E. coli) [Msh2] gene. Homozygous Msh2LoxP mice are viable and fertile with no observed abnormalities. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding a portion of the essential ATPase domain of MSH2 protein deleted in the cre-expressing tissue(s). These Msh2LoxP mice may be useful in generating tissue-specific MSH2 deletions for studying DNA mismatch repair (single-nucleotide and insertion/deletion mismatches), as well as tumor development.

For example, when Msh2LoxP mice are bred to a strain expressing Cre recombinase in embryonic tissues (EIIA-Cre; see Stock Nos. 003314 or 003724), the resulting mice with pan deletion of MSH2 exhibit high inciden .....
For more information please see the full phenotype on the strain data sheet

021879 B6.Cg-Snap25tm1.1Hze/J
Cryopreserved - Ready for recovery
006922 B6.Cg-Spi1tm2Dgt/J
Cryopreserved - Ready for recovery
Mice that are homozygous for this "PU.1F" conditional allele are viable and fertile. When PU.1F mice are bred to mice expressing Cre recombinase, exons 4-5 of the targeted gene are deleted in the cre-expressing tissues in the offspring. These mice may be useful in studying T cell lymphoma, AML and other cancers, as well as transcription factors, hematopoiesis, and the development of multiple cell lineages.

For example, when PU.1F mice are crossed with mice expressing the interferon- or dsRNA-inducible Mx1-cre transgenic mice (see Stock No. 003556), this mutant mouse strain may be useful in studies of hematopoietic stem cells.

NOTE: Despite these mice being backcrossed onto the C57BL/6 genetic background, occasional albino pups may be observed. The donating investigator confirms this observation and suggests the targeted mutation may have an as of yet uncharacterized effect upon coat color .....
For more information please see the full phenotype on the strain data sheet

012627 B6.Cg-Stard4tm1.1Bres/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in cre-expressing tissues.
021506 B6.Cg-Uba6tm1Whpr/J
Cryopreserved - Ready for recovery
These Uba6flox/flox mutant mice possess loxP sites flanking exons 16-17 of the ubiquitin-like modifier activating enzyme 6 (Uba6) gene. Uba6 (E1) is part of the ubiquitin proteasome system (UPS) which regulates protein homeostasis during development. Complete UBA6 deficiency leads to embryonic arrest by E5.5. Mice that are homozygous for this floxed-allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 3-6 deleted in the cre-expressing tissues.

For example, when bred to B6.Cg-Tg(Nes-cre)1Kln/J mice (Stock No. 003771), resulting mice lack neuronal UBA6 and display altered neuronal patterning in the hippocampus and the amygdala, decreased dendritic spine density, and numerous behavioral disorders. They also contain altered levels of ubiquitin protein ligase E3A (Ube3a) and SH3/ankyrin domain gen .....
For more information please see the full phenotype on the strain data sheet

009369 B6.Cg-Zbtb7btm1.1Litt/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.

Deletion in CD4+CD8+ thymocytes (through crosses to a Cd4-cre strain) or germline deletion (through EIIa-cre crosses; see Stock No. 003724) causes mice to lose helper T cells. This strain may be useful in studies of T cell lineage commitment.

022413 B6.Cg-Zfp335tm1Caw
Cryopreserved - Ready for recovery
Zfp335 (zinc finger protein 335; also known as a NRC-interacting factor 1 (Nif1)) is a regulator of vertebrate neurogenesis. As a component of the trithorax H3K4-methylation complex that regulates REST/NRSF, it is essential for neural cell progenitor self-renewal, neurogenesis, and neuronal differentiation. A c.3332g>a mutation in the human gene is associated with severe microcephaly, neuronal degeneration, and neonatal death.

In this targeted mutation strain, the Zfp335 promoter, exon 1, exon 2, and an Frt-flanked neomycin cassette are flanked by loxP sites. Cre-mediated excision of this floxed region enables the generation of tissue-specific knockouts. Widespread loss of ZNF335 protein in mice leads to embryonic lethality as early as embryonic day 7.5 (E7.5).

When crossed with Emx1-cre mice (see Stock No. 005628), progeny lack almost all cortical structure and cortical neurons, leading to the formati> .....
For more information please see the full phenotype on the strain data sheet

008705 B6.Cg-Tg(CAG-DsRed,-EGFP)5Gae/J
Cryopreserved - Ready for recovery
Hemizygous IRG transgenic mice are viable and fertile, with widespread expression of a loxP-flanked optimized red fluorescent protein variant (DsRed-Express) directed to embryonic and adult tissues by the CAG promoter prior to exposure to Cre recombinase. When bred to cre-expressing mice, the resulting offspring have the DsRed-Express cassette deleted in the cre-expressing tissue(s), allowing expression of the enhanced green fluorescent protein (EGFP) cassette located just downstream. These IRG transgenic mice are a double-fluorescent, Cre-reporter strain; with widespread expression of red fluorescence prior to Cre recombinase exposure, and green fluorescence following cre-mediated recombination in a pattern determined by cre expression, and should provide a versatile tool for analyzing complex cellular relationships in a wide variety of tissues.

For example, when using IRG transgenic mice along with Nestin-Cre mice (see Stock No. For more information please see the full phenotype on the strain data sheet

016882 B6.Cg-Tg(CMV-CASP3)14Edge/J
Cryopreserved - Ready for recovery
Mos-iCsp3 transgenic mice have a CMV promoter directing expression of a floxed-lacZ-STOP cassette followed by two tandem FK506-binding sites (Fvs) and a downstream human Caspase 3 (Casp3) gene. Hemizygous Mos-iCsp3 mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Caspase 3 is a member of the cysteine-aspartic acid protease family of enzymes which are integral to apoptosis pathways. Inactive until cleaved by an initiator enzyme, Caspase 3 is processed at conserved aspartic residues and is activated by the formation of dimers. In this transgenic strain, a STOP cassette is present, flanked by a loxH site and a loxP site, preventing inducible Caspase 3 expression and allowing for widespread lacZ staining. LacZ staining in founder line 14 is seen in the eye, kidney, pancreas, skin, thymus, and portions of the brain. The presence of both a loxH site and a loxP site causes reduc .....
For more information please see the full phenotype on the strain data sheet
016908 B6.Cg-Tg(CMV-CASP3)17Edge/J
Cryopreserved - Ready for recovery
Mos-iCsp3 transgenic mice have a CMV promoter directing expression of a floxed-lacZ-STOP cassette followed by two tandem FK506-binding sites (Fvs) and a downstream human Caspase 3 (Casp3) gene. Hemizygous Mos-iCsp3 mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Caspase 3 is a member of the cysteine-aspartic acid protease family of enzymes which are integral to apoptosis pathways. Inactive until cleaved by an initiator enzyme, Caspase 3 is processed at conserved aspartic residues and is activated by the formation of dimers. In this transgenic strain, a STOP cassette is present, flanked by a loxH site and a loxP site, preventing inducible Caspase 3 expression and allowing for widespread lacZ staining. LacZ staining in founder line 17 is seen in the spinal cord, muscle, pancreas, skin, and portions of the brain and skull. The presence of both a loxH site and a loxP site cau .....
For more information please see the full phenotype on the strain data sheet
016149 B6.Cg-Tg(SOD1*G37R)1Dwc/J
Cryopreserved - Ready for recovery
The LoxSOD1G37R transgene has loxP sites flanking a human G37R mutant superoxide dismutase 1, soluble gene sequence (SOD1*G37R) that is characterized as an enzymatically active, "gain of adverse function" mutation. SOD1 is a widely expressed isozyme responsible for destroying free superoxide radicals. SOD1 mutations, including SOD1*G37R, are known to cause Amyotrophic Lateral Sclerosis (ALS). Hemizygotes mice are viable and fertile. These mice develop symptoms and pathology resembling human ALS, including progressive weight loss from denervation-induced muscle atrophy and paralyzation. Transgenic mice develop the fatal progressive motor neuron disease and die by 12-14 months of age, with neuron death in 55% of spinal motor neurons.
When LoxSOD1G37R transgenic mice are bred to mice expressing tissue-specific Cre recombinase, the resulting offspring will have the floxed-SOD1*G37R transgene deleted in the cre-expressing tissue .....
For more information please see the full phenotype on the strain data sheet
007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J
Cryopreserved - Ready for recovery
These Thy1-Brainbow 1.1 (line M) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Although the fluorescent protein immediately adjacent to the promoter, Kusabira-Orange (kOFP), was designed to be expressed prior to Cre-mediated recombination, basal kOFP expression is not observed in mouse tissues. When bred to Cre recombinase expressing mice, however, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): mCerulean (CFP), mYFP, or mCherry (RFP). The resulting fluorescent protein expression is observed in astrocytes of all areas of the brain and spinal cord, as well as dentate gyrus granule cells. A palmitoylation sequence tethers the mCherry (RFP), .....
For more information please see the full phenotype on the strain data sheet
007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J
Cryopreserved - Ready for recovery
These Thy1-Brainbow 2.1 (line R) transgenic mice are viable and fertile. The mice possess two invertible DNA segments (four fluorescent protein sequences in total) uniquely positioned in tandem and flanked with LoxP sites to generate a larger number of recombination outcomes; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Although the fluorescent protein immediately adjacent to the promoter, hrGFPII (with nuclear localization signal), was designed to be expressed prior to Cre-mediated recombination, basal hrGFPII expression may not be observed in mouse tissues. When bred to Cre recombinase expressing mice, however, the resulting offspring can have one of three different inversions for each transgene in each cell of the cre expressing tissue(s). In addition, two excision events may reduce the construct to one of two single invertible DNA segments which can continue to invert as long as cre is present. These different recomb .....
For more information please see the full phenotype on the strain data sheet
005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J
Cryopreserved - Ready for recovery
These transgenic mice conditionally express Enhanced Yellow Fluorescent Protein (EYFP) under the direction of the mouse thymus cell antigen 1, theta, promoter. Expression of the EYFP gene is blocked by a loxP-flanked STOP fragment placed between the promoter and EYFP gene. Cre-mediated excision of the STOP cassette results in expression of EYFP in motor neurons. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

For example, when crossed to a strain with widespread expression of Cre recombinase (see Stock No. 003376), this mutant mouse strain may be useful in studies of synaptic function.

015806 B6.Cg-Tg(UBC-GFP,-TVA)2Clc/J
Cryopreserved - Ready for recovery
The cTVA transgene contains the human ubiquitin C (UBC) promoter/enhancer elements drive expression of a loxP-flanked green fluorescent protein (GFP) and polyadenylation sequence, followed by an avian specific retroviral receptor (TVA) gene derived from quail. Hemizygous mice are viable and fertile. UBC is a polyubiquitin precursor responsible for the regulation of cell signaling pathways upon conjugation with various proteins. In this mutant mouse, GFP is expressed in UBC-expressing cells. When bred to mice that express Cre recombinase, offspring will have the floxed-GFP-STOP cassette deleted in the cre-expressing tissue(s), resulting in TVA overexpression in UBC-expressing cells. Upon expression of TVA, these cells are capable of binding viruses with the envelope protein of avian sarcoma/leukosis virus subtype A (ASLV-A). These cTVA mice may be useful for infection by retroviruses, rabies virus, and other viruses with ASLV-A .....
For more information please see the full phenotype on the strain data sheet
015807 B6.Cg-Tg(UBC-GFP,-TVA)3Clc/J
Cryopreserved - Ready for recovery
The cTVA transgene contains the human ubiquitin C (UBC) promoter/enhancer elements drive expression of a loxP-flanked green fluorescent protein (GFP) and polyadenylation sequence, followed by an avian specific retroviral receptor (TVA) gene derived from quail. Hemizygous mice are viable and fertile. UBC is a polyubiquitin precursor responsible for the regulation of cell signaling pathways upon conjugation with various proteins. In this mutant mouse, GFP is expressed in UBC-expressing cells. When bred to mice that express Cre recombinase, offspring will have the floxed-GFP-STOP cassette deleted in the cre-expressing tissue(s), resulting in TVA overexpression in UBC-expressing cells. Upon expression of TVA, these cells are capable of binding viruses with the envelope protein of avian sarcoma/leukosis virus subtype A (ASLV-A). These cTVA mice may be useful for infection by retroviruses, rabies virus, and other viruses with ASLV-A .....
For more information please see the full phenotype on the strain data sheet
002982 B6.Cg-Tg(xstpx-lacZ)32And/J
Cryopreserved - Ready for recovery
This mutant, when crossed with a cre transgenic, will express lacZ in cells where cre is expressed to remove the STOP of translation section which lies between the 2 loxP sites. LacZ expression is restricted to neural and skeletal muscle tissue and heart by the chicken beta-actin promoter that is driving the reporter.
008226 B6.FVB-Tg(CAG-EGFP,-ALPP)2.6Ggc/J
Cryopreserved - Ready for recovery
Mice harboring the piGAP transgene are viable and fertile, with expression of the eGFP-F-IRES-hPLAP dicistronic gene blocked by an unpstream loxP-flanked STOP-polyA sequence. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP-polyA sequence deleted in the cre-expressing tissue(s), permitting dicistronic expression of eGFP-F-IRES-hPLAP. When transgene expression is induced in neurons, human Placental Alkaline Phosphatase (PLAP or ALPP) outlines axonal and dendritic projections and can be visualized by a simple histochemical reaction in fixed cells. Likewise, in vivo fluorescence of farnesylated Enhanced Green Fluorescent Protein (eGFP-F; optimized to target expression to the cytoplasmic side of the plasma membrane) labels axons, and dendrites throughout their length. Because both proteins localize alongside the neuronal surface, concomitant detection of cell body, neurites, and presynaptic and postsynaptic sites may be .....
For more information please see the full phenotype on the strain data sheet
022503 B6;129-Adcy6tm1.1Dek/J
Cryopreserved - Ready for recovery
These Adcy6flox mutant mice possess loxP sites flanking exons 3-12 of the adenylate cyclase 6 (Adcy6) gene. Adcy6 encodes AC6, an enzyme required for formation of cyclic adenosine monophosphate (cAMP) involved intracellular signal transduction. cAMP plays a role in the cellular functions such as the trafficking of proteins and the activation of ion channels. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 3-12 deleted in the cre-expressing tissues.

For example, when bred to B6.Cg-Tg(Aqp2-cre)1Dek/J mice (Stock No. 006881) expressing Cre recombinase in the collecting duct of the kidney, double mutant mice have reduced urine osmolality under normal and water deprivation conditions.

010687 B6;129-Adora2atm1Dyj/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the critical exon deleted in the cre-expressing tissues.

When bred to a strain expressing Cre recombinase in the forebrain (see Stock No. 005359 for example), this mutant mouse strain may be useful in studies of locomoter activity response to addictive substances.

017746 B6;129-Apaf1tm1Hez/J
Cryopreserved - Ready for recovery
Apoptotic protease activating factor 1 and caspase 9 together form the apoptosome complex, which activates and initiates the apoptotic pathway. Loss of apoptosome function may be involved in the development of some cancers and is implicated in tumor drug resistance. These mice possess loxP sites on either side of exon 5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 5 deleted in the cre-expressing tissue(s). Deletion of exon 5 is expected to result in expression of only the first 176 amino acid residues of the protein.
006394 B6;129-Apba2tm1Sud Apba3tm1Sud Apba1tm1Sud/J
Cryopreserved - Ready for recovery
Triple homozygous knock-in mice are viable and fertile and and do not display any gross physical or behavioral abnormalities. Expression of all three proteins is normal. When crossed with a cre deleter strain that eradicates protein expression, Apba1/Abcba2 (Apba1/2) double knockout and Apba1/2/3 triple knockout mice exhibit a high percentage of postnatal lethality (only ~20% of the mice survive). Apba1/2 mice are visually indistinguishable from their littermates and display no major alterations in breathing, movements or reaction to stimuli several hours after birth, but fail to nurse and die within 24 hours. Their brains are morphologically and structurally normal. Quantitation of 18 neuronal proteins fails to reveal significant changes. Surviving mice show reduced weight gain and exhibit movement dysfunction. Cultured neurons from triple knock-in neonates show impairments in presynaptic neurotransmitter release after treatment with lentiviral cre. Apba1/3 .....
For more information please see the full phenotype on the strain data sheet
006329 B6;129-Baxtm2Sjk Bak1tm1Thsn/J
Cryopreserved - Ready for recovery
Mice homozygous for both alleles (Baxfl and bak-) are viable and fertile with no reported abnormalities. Splenic and thymic tissues display no Bak1 protein expression.

When bred to Cre recombinase expressing mice, the resulting offspring will have exons 2-4 of Bax deleted in the cre-expressing tissues (determined by promoter driving cre expression). The conditional deletion of Bax combined with the Bak1 null allele makes these mice useful in studies of apoptosis regulation, tissue homeostasis, and development in multiple cell lineages.

When bred to a strain with a Bak1 targeted null allele (Stock No. 004183) and to either a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 004126) or to a strain expressing interferon inducible Cre recombinase ( .....
For more information please see the full phenotype on the strain data sheet

006382 B6;129-Casktm1Sud/J
Cryopreserved - Ready for recovery
Homozygous floxed mice are viable and fertile, but females do not thrive. The body size of mutants is significantly smaller than littermate controls and they exhibit a slightly increased mortality. Knock-in mice are hypomorphs and protein is expressed at less than 30% of normal levels. Crossing of the floxed mutants with mice expressing cre recombinase in the male germline excises the floxed exon and a neomycin resistance gene cassette to create a complete knockout of the gene. Knockout homozygotes die within a few hours of birth. They exhibit a partially penetrant cleft palate syndrome and increased apoptosis in the thalamus, but display no other major developmental changes or deficits in basic electrical properties of their neurons.

When bred to a strain expressing Cre recombinase in the male germline (see Stock No. 003328 or 007252 for example), this mutant mouse str .....
For more information please see the full phenotype on the strain data sheet

004604 B6;129-Ctnna1tm1Efu/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

When bred to a strain expressing Cre recombinase in the female germline (see Stock No. 003551 for example), this mutant mouse strain may be useful in studies of mammary epithelium.

When bred to a strain expressing Cre recombinase in the mammary gland (see Stock No. 003552 for example), this mutant mouse strain may be useful in studies of mammary epithelium.

When bred to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771 for example), this mutant mouse strain may be useful in studies of the cerebral cortex and the hedgehog signalling pathway.

Wh .....
For more information please see the full phenotype on the strain data sheet

013097 B6;129-Dlg1tm1Rlh/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of an exon encoding portions of both the PDZ1 and PDZ2 domains of the Dlg1 (discs, large homolog 1 (Drosophila); SAP97) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.

Widespread deletion of expression leads to neonatal lethality due to failure of maxillar bone fusion. If deletion is specific to motor neurons (Stock No. 006600), neurite extension is suppressed. This strain may be useful for further characterizaton of the targeted gene's synaptic function.

When bred to mice carrying Tg(Cd4-cre)1Cwi (Stock No. 017336), Cre recombinase expression in the CD4-expressing T .....
For more information please see the full phenotype on the strain data sheet

013098 B6;129-Dlg3tm1Rlh/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of six exons encoding the PDZ1 and PDZ2 domains of the Dlg3 (discs, large homolog 3 (Drosophila); SAP102) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. Defective expression of this gene is associated with deficits in synaptic plasticity and deterioration of learning/memory functions.
011082 B6;129-Edn2tm1.1Nat/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of the second exon in the Edn2 (endothelin 2) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. When crossed with a Sox2-Cre transgenic strain, mice homozygous for the resulting allele lack Edn2 expression. This strain may be useful in elucidating the many roles of this gene.
011080 B6;129-Ednrbtm1.1Nat/J
Cryopreserved - Ready for recovery
These mice possess loxP sites flanking portions of exon 2 and intron 2 of the Ednrb (endothelin receptor type B) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful for studying the variety of functions associated with this gene ranging from coat color to cell signaling, digestive and immune phenotypes.

When crossed with a Sox2-cre transgenic strain, mice homozygous for the resulting allele lack Ednrb expression and have a phenotype identical to mice with the spotted lethal (s-l, piebald lethal) allele.

008452 B6;129-Eif2ak4tm1.1Dron/J
Cryopreserved - Ready for recovery
Mice homozygous for the GCN2.KO4conditional allele (also called GCN2.KO4c) are viable and fertile, with loxP sites flanking exon XII of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (containing amino acid residues 606-648 encoding the critical lysine 618 required for kinase activity) deleted in the cre-expressing tissue(s). GCN2 is a protein kinase that phosphorylates eIF2 (eukaryotic initiation factor 2) in response to environmental stresses (amino acid starvation, proteasome inhibition and UV irradiation) resulting in a reduction of global translation and activation of stress-related transcription factors (such as NF-kappaB). These GCN2.KO4conditional mice may be useful in studies related to eIF2 phosphorylation in response to environmental stresses.

Of note, mice with a traditional "knockout" (deletion of exon XII) are also available (see Stock No. For more information please see the full phenotype on the strain data sheet

009326 B6;129-Ext1tm1Vcs/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissues. Spurs of extra bone develop in progeny derived from a cross with an inducible Col2a1 collagen promoter Cre strain.
023900 B6;129-Fgf3tm1.2Sms/J
Cryopreserved - Ready for recovery
These Fgf3c mice possess loxP sites flanking exon 2 of the fibroblast growth factor 3 (Fgf3) gene. The donating investigator reports that a tetracycline response element present upstream of exon 2 has no effect on expression unless a tetO binding protein is present and activated. FGF3 is expressed in the hindbrain, pharyngeal endoderm and tailbud, and is involved in cell division, embryonic development, regulation of cell growth and maturation, formation of blood vessels, and wound healing. Homozygotes are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

For example, when bred to BALB/c-Tg(CMV-cre)1Cgn/J mice (Stock No. 003465), FGF3 null mice fail to initiate inner ear and limb development.

011078 B6;129-Fzd4tm2.1Nat/J
Cryopreserved - Ready for recovery
This strain combines a conditional knockout and an alkaline phosphatase (AP) reporter of the frizzled 4 (Fzd4; Fz4) gene. LoxP sites flank the 5' and 3' UTR's. Without recombination, homozygotes of this allele are indistinguishable from wild type mice and the AP reporter is not expressed. Following cre-mediated recombination, the coding region is excised, and the downstream AP reporter is expressed under the control of the Fzd4 promoter in a cell/tissue-specific manner. Loss of Fzd4 signaling causes defective vascular growth which leads to chronic but reversible silencing of retinal neurons. Loss of expression in all endothelial cells disrupts the blood brain barrier in the cerebellum. This strain may be useful in studies of vascular growth, remodeling, maintenance and disease.
008620 B6;129-Fzd5tm2Nat/J
Cryopreserved - Ready for recovery
This is a conditional allele of the frizzled 5 gene in which the coding region was flanked by loxP sites and an alkaline phosphatase (AP) reporter was placed downstream. Cre-mediated recombination eliminates the coding region in a tissue-specific manner, ablates expression of the gene, and causes alkaline phosphatase (AP) to be expressed under the control of the frizzled 5 promoter.

If both floxed alleles are excised, mutants are embryonic lethal at day 10 due to a placental defect. If recombination is mediated by an embryonic tissue-specific Cre, such as Sox2-Cre (e.g. Stock No's. 004783, 008454), mice show a loss of cells in the parafasicular nucleus (PFN) of the thalamus and defects in eye development.

When bred to a strain expressing a tamoxifen inducible Cre recombinase (see Stock No. 004847 .....
For more information please see the full phenotype on the strain data sheet

008310 B6;129-Gabrb3tm2.1Geh/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s).

When bred to a strain expressing Cre recombinase in the neuronal cells (see Stock No. 003966 for example), this mutant mouse strain may be useful in studies of neurodevelopmental diseases.

When bred to a strain expressing Cre recombinase in the hippocampal CA1 pyramidal cell layer (see Stock No. 005359 for example), this mutant mouse strain may be useful in studies of neurodevelopmental diseases.

Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations .....
For more information please see the full phenotype on the strain data sheet

009076 B6;129-Gcnt1tm2Jxm/J
Cryopreserved - Ready for recovery
008883 B6;129-Gt(ROSA)26Sortm1(SNCA*A53T)Djmo/TmdJ
Cryopreserved - Ready for recovery
Homozygous ROSA26-Syn-A53T mice are viable and fertile, with the familial Parkinson's disease-associated A53T missense mutant form of human alpha-synuclein (human A53T α-Syn or SYNA53T) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of human A53T α-Syn is blocked by an upstream loxP-flanked STOP sequence (in the absence of Cre recombinase, no human A53T α-Syn protein is observed in brain regions). When bred to cre expressing mice, the STOP sequence is deleted in the tissues of offspring where Cre recombinase is present; resulting in human A53T α-Syn expression. In particular, Stock No. 008601 B6.Cg-Tg(Th-cre)1Tmd/J may be useful for this application. These ROSA26-Syn-A53T mice allow inducible expression of a human mutation associated with familial Parkinson's disease and may be useful for studying the progressive dopaminergic neurodegeneration of Parkinson's dise .....
For more information please see the full phenotype on the strain data sheet
008516 B6;129-Gt(ROSA)26Sortm1Joe/J
Cryopreserved - Ready for recovery
Homozygous ROSA26 GNZ knock-in mice are viable and fertile, with a nuclear-localized green fluorescent protein/beta-galactosidase fusion protein (GFP-NLS-lacZ or GNZ) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of GNZ is blocked by an upstream loxP-flanked STOP sequence (in the absence of Cre recombinase, no expressed GFP or beta-galactosidase activity is observed in GNZ embryos(E9.5-18.5)). When bred to cre expressing mice, offspring will have the STOP sequence deleted in tissues where Cre recombinase is present. The resulting GNZ fusion protein expression allows for enhanced (single cell level) visualization / resolution. The donating investigator reports that Cre recombinase activity can be visualized by direct GFP fluorescence, but the high resolution nuclear staining of GNZ may be best visualized by immunostaining for either GFP or beta-galactosidase. These ROSA26 GNZ mice are useful as a Cre reporter strain; expressing both G .....
For more information please see the full phenotype on the strain data sheet
003504 B6;129-Gt(ROSA)26Sortm1Sho/J
Cryopreserved - Ready for recovery
Mice with the Gt(ROSA)26tm1Sho targeted mutation are similar to the B6;129-Gt(ROSA)26tm1Sor from Dr. Philippe Soriano, but reported in the literature to be an improved reporter strain for monitoring cre-mediated excisions (see Important Note). The β-galactosidase-neomycin phosphotransferase fusion gene (βgeo)-trapped reverse orientation splice acceptor βgeo line 26 (ROSA26) locus was modified by gene targeting such that βgeo is expressed only after Cre-mediated excision of loxP-flanked DNA sequences. βgeo from the excised ROSA26 allele is expressed ubiquitously in embryos and adult mice. When mating the reporter strain with cre-expressing transgenic mice, one can see that the loxP-flanked ROSA26 allele is accessible to cre during early embryogenesis, as well as in a specific hematopoietic lineage (T lymphocytes). These mice may prove useful in the study of cell fate and cell migration during .....
For more information please see the full phenotype on the strain data sheet
008889 B6;129-Gt(ROSA)26Sortm2(SNCA*119)Djmo/TmdJ
Cryopreserved - Ready for recovery
Homozygous ROSA26-Syn119 mice are viable and fertile, with the familial Parkinson's disease-associated Syn119 C-terminal truncation of human alpha-synuclein (human α-Syn119 or SynCT119) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of human α-Syn119 is blocked by an upstream loxP-flanked STOP sequence (in the absence of Cre recombinase, no human α-Syn119 protein is observed in brain regions). When bred to cre expressing mice, the STOP sequence is deleted in the tissues of offspring where Cre recombinase is present; resulting in human α-Syn119 expression. In particular, Stock No. 008601 B6.Cg-Tg(Th-cre)1Tmd/J may be useful for this application. These ROSA26-Syn119 mice allow inducible expression of a human mutation associated with familial Parkinson's disease and may be useful for studying the progressive dopaminergic neurodegeneration of Parkinson's disease and .....
For more information please see the full phenotype on the strain data sheet
009253 B6;129-Gt(ROSA)26Sortm2Nat/J
Cryopreserved - Ready for recovery
This strain carries an inverted human placental alkaline phosphatase (ALPP; PLAP) coding segment flanked by oppositely-oriented loxP sites integrated in the Gt(ROSA)26Sor gene. Cre excision of the floxed segment flips rather than excises the ALPP inversion and reinstates a transcriptionally active form of the gene. This reporter can be activated by tissue-specific cre recombinase in any cell in the body at any time in development. The result is very clear ALPP enzyme activity that can be detected histochemically. Heterozygotes and homozygotes are normal in size, viability, and fertility.
008886 B6;129-Gt(ROSA)26Sortm3(SNCA*E46K)Djmo/TmdJ
Cryopreserved - Ready for recovery
Homozygous ROSA26-Syn-E46K mice are viable and fertile, with the E46K missense mutant form of human alpha-synuclein (human E46K α-Syn or SYNE46K; associated with familial Parkinson's disease, dementia, and visual hallucinations) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of human E46K α-Syn is blocked by an upstream loxP-flanked STOP sequence (in the absence of Cre recombinase, no human E46K α-Syn protein is observed in brain regions). When bred to cre expressing mice, the STOP sequence is deleted in the tissues of offspring where Cre recombinase is present; resulting in human E46K α-Syn expression. In particular, Stock No. 008601 B6.Cg-Tg(Th-cre)1Tmd/J may be useful for this application. These ROSA26-Syn-E46K mice allow inducible expression of a human mutation associated with familial Parkinson's disease, dementia, and visual hallucinations and may be usefu .....
For more information please see the full phenotype on the strain data sheet
010590 B6;129-Iis1tm1(CAG-Bgeo,-tdTomato/TEVP,-SV2B/GFP)Nat/J
Cryopreserved - Ready for recovery
A targeted insertion placed 2 kb 5' of the ubiquitin b (Ubb) gene provides constitutive expression of a nuclear-localized beta-galactosidase/neomycin resistance protein reporter (NLS-beta-geo) in the absence of Cre-mediated recombination. Following Cre-mediated recombination, the NLS-beta-geo coding region is excised and a downstream open reading frame is expressed. The downstream open reading frame codes for a single large protein - tdTomato/Tobacco etch virus protease (TEVP), a 6xMyc epitope, a TEVP cleavage site, synaptic vesicle protein 2B (SV2B), GFP, 3xHA (hemagglutinin) epitope - which cleaves itself into two pieces shortly after translation. When Cre-mediated recombination occurs in neurons, the amino terminal piece (tdTomato/TEVP, a 6xmyc epitope, a TEVP cleavage site) labels all axon and dendrites, and the carboxy-terminal portion (SV2B, GFP, 3xHA epitope) labels presynaptic structures. This reporter can be activated by cre recombinase in any cell in the body at any .....
For more information please see the full phenotype on the strain data sheet
011077 B6;129-Iis1tm2(CAG-Bgeo,-Ndp,-EGFP)Nat/J
Cryopreserved - Ready for recovery
A targeted insertion located 2 kb 5' of the Ubb ubiquitin b gene (the integration site of the Z/AP reporter) gives constitutive expression of a nuclear-localized beta-galactosidase/neomycin resistance protein reporter (NLS-beta-geo) in the absence of Cre-mediated recombination. Following cre-mediated recombination, the NLS-beta-geo coding region is excised and a downstream open reading frame is expressed. The downstream open reading frame codes two proteins - Norrin (NDP), and GFP with 6xMyc epitope, but the GFP coding region, which follows an IRES, is expressed at very low levels. After cre-mediated recombination in the retina, ectopic Norrin expressed from this locus can rescue the vascular defect caused by a Norrin null mutation.

For example, when crossed to a strain expressing Cre recombinase in epiblast cells (see Stock No. 008454), this mutant mouse strain may be useful in studies of retinal vascularization. .....
For more information please see the full phenotype on the strain data sheet

012587 B6;129-Iis1tm3(CAG-Bgeo,-tdTomato/TEVP,-Dlg4,-GFP)Nat/J
Cryopreserved - Ready for recovery
A CMV/beta actin (Z/AP) promoter located 2 kb 5' of the Ubb (ubiquitin B) gene drives constitutive expression of a nuclear-localized beta-galactosidase/neomycin resistance protein reporter (NLS-beta-geo) in the absence of Cre-mediated recombination. Following Cre-mediated recombination, the NLS-beta-geo coding region is excised and a downstream open reading frame is expressed. The downstream open reading frame codes for a single large protein (tdTomato/Tobacco etch virus protease (TEVP), a 6xMyc epitope, a TEVP cleavage site, post-synaptic density protein (PSD95) fused to green fluorescence protein (GFP) with a 3xHA epitope) which cleaves itself into two pieces shortly after translation. When Cre-mediated recombination occurs in neurons, the amino terminal piece (tdTomato/TEVP, a 6xMyc epitope, a TEVP cleavage site) labels all axon and dendrites, and the carboxy-terminal portion (PSD95, GFP, 3xHA epitope) labels post-synaptic structures.
016240 B6;129-Kiztm1Cpl/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 5 and 6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 5 and 6 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in primordial germ cells (see Stock No. 008569 for example), this mutant mouse strain may be useful in studies of mammalian germ cell differentiation.

012894 B6;129-Macf1tm1Efu/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 6 and 7 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 6 and 7 deleted in the cre-expressing tissue(s).
006885 B6;129-Man2a1tm2Jxm/J
Cryopreserved - Ready for recovery
Mice homozygous for this floxed targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. When bred to Cre transgenic strains, the loxP-flanked exon is recombined and deleted where Cre is expressed, producing a null allele.
006088 B6;129-Mcl1tm3Sjk/J
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator indicates that homozygous males have severely reduced fertility for unknown reasons, while females have normal fertility. Endogenous protein expression is unaffected by the inserted loxP sequences. When bred to mice with a cre recombinase gene under the control of a promoter of interest, exon 1 of the targeted gene is deleted in the tissue of interest. These mutant mice may be useful in studying global, temporal, or tissue-specific deletion of the endogenous gene, particularly in studies of immune function, including apoptosis, B and T cell development, and bone marrow cell differentiation.

When bred to a strain with the targeted null allele (Stock No. 006072) and a strain with a Cd19 null allele and expressing Cre recombinase during th .....
For more information please see the full phenotype on the strain data sheet

006894 B6;129-Mgat4atm1Jxm/J
Cryopreserved - Ready for recovery
Mice homozygous for this floxed targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exon is deleted by cre expression to produce a null allele.
006933 B6;129-Mycntm1Psk/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 and 3 deleted in the cre-expressing tissue(s).

For example, when crossed to a strain expressing Cre recombinase in neuronal and glial cell precursors (see Stock No. 003771), this mutant mouse strain may be useful in studies of neurogenesis.

010607 B6;129-Myrftm1Barr/J
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile with loxP sites flanking exon 8 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in cre-expressing tissue(s). This mutant mouse strain may be useful in generating conditional mutations for studying the role of Gm98 in CNS myelination and other cellular processes.

For example, when crossed to a strain expressing Cre recombinase in oligodendrocytes(see Stock No. 011103), this mutant mouse strain may be useful in studies of oligodendrocyte maturation and CNS myelination.

008476 B6;129-Ncstntm1Sud/J
Cryopreserved - Ready for recovery
Floxed homozygous mice express intact nicastrin gene. They are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s). General deletion in all tissues results in E9.0 embryonic lethality. g-secretase activity is abolished in cells derived from homozygous cre-recombined mice. These mice are useful to study the role of g-secretase in various tissues in combination with tissue specific cre lines.
008475 B6;129-Nlgn3tm1Sud/J
Cryopreserved - Ready for recovery
These mice carry an R451C mutation in exon 7 of the gene. mRNA is detected by real-time PCR analysis of brain from homozygous animals. Mutant mice exhibit enhancements in inhibitory synaptic transmission as well as spacial learning and memory, but show deficits in social interaction. This mutant mouse strain may be useful in studies of the pathophysiology of autism. Exon 7 is additionally flanked by loxP sites. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical abnormalities.
014157 B6;129-Nrxn3tm3Sud/J
Cryopreserved - Ready for recovery
These mice carry a floxed allele of the Nrxn3 (neurexin III) gene. When bred to mice that express Cre recombinase, resulting offspring will have exon 18 deleted in the cre-expressing tissues, blocking expression of the alpha and beta transcripts. Homozygous floxed mice are viable, fertile and do not display any gross physical or behavioral abnormalities. Gene expression of the floxed allele is reportedly normal in brain tissue.
016194 B6;129-Nrxn3tm4.1Sud/J
Cryopreserved - Ready for recovery
These NS4 mutant mice possess loxP sites flanking exon 20 (alternative splice site 4) of the neurexin III (Nrxn3) gene, and a mutated exon 20 splice acceptor sequence. Mice that are homozygous for this allele are viable, fertile, and normal in size. NRXN3, an intercellular cell signaling protein, is expressed in neurons involved in addictive behaviors and is associated with nicotine and opioid dependence and substance abuse. Nrxn3 has two distinct promoters and five alternative splice sites. These Nrxn3 mice always incorporate alternative splice site 4 in the transcript. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have this mutated exon 20 deleted in the cre-expressing tissues. This strain may be useful for studying neuronal synapses related to alcoholism, dependence, and addiction behaviors.
011081 B6;129-Osmrtm1.1Nat/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of the second exon (first coding exon) in the Osmr (oncostatin M receptor) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. Expression in the retina and retinal pigment epithelium (RPE) is undetectable in protein blots after crosses with a germline cre strain. This strain may be useful in further characterization of this multifunctional cytokine.
012653 B6;129-Pax7tm1.1Fan/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s).

For example, when crossed to a strain expressing tamoxifen-inducible Cre recombinase in myogenic progenitor cells (see Stock No. 012476), this mutant mouse strain may be useful in studies muscle stem cells.

019143 B6;129-Paxip1tm2Gdr/J
Cryopreserved - Ready for recovery
Paxip1, PAX interacting (with transcription-activation domain) protein 1, (PTIP) associates with histone H3K4 methyltransferases and is involved in DNA damage response, transcriptional regulation and progression through mitosis. These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in the renal inner medulla, this mutant mouse strain may be useful in studies of renal physiology, urine concentration and osmotic tolerance.

018319 B6;129-Plxnd1tm1.1Tmj/J
Cryopreserved - Ready for recovery
These plexinD1flox mutant mice possess loxP sites flanking exon 1 of the plexin D1 (Plxnd1) gene. PlexinD1 is a transmembrane receptor for class 3 semaphorins expressed prominently in developing vascular endothelial cells. Expression is also seen in the central nervous system, in the salivary gland, neural crest and in bone during embryogenesis. PlexinD1 has been found to play a role in the migration and proliferation of some vascular tumor cells. Homozygous plexinD1flox mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissues, abolishing gene function.

For example when bred to B6.Cg-Tg(Tek-cre)1Ywa/J mice (Stock No. 008863), expressing cre-recombinase in the vascular endothe .....
For more information please see the full phenotype on the strain data sheet

014168 B6;129-Pot1atm1.1Tdl/J
Cryopreserved - Ready for recovery
These targeted mutant mice possess loxP sites on either side of the third coding exon of the Pot1a (protection of telomeres 1A) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, resulting offspring will not express the targeted gene in cre-expressing tissues.
014169 B6;129-Pot1btm1.1Tdl/J
Cryopreserved - Ready for recovery
These targeted mutant mice possess loxP sites on either side of the third coding exon of the Pot1b (protection of telomeres 1B) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in studies of telomere biology.
010558 B6;129-Pou4f1tm2.1Nat/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of the Pou4f1 (POU domain, class 4, transcription factor 1) coding region followed by an alkaline phosphatase (AP) reporter. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a cre recombinase-expressing strain, the offspring express AP in the retina, several brain nuclei, dorsal root ganglia, trigeminal ganglion, and other cells/tissues. AP enzyme activity can be clearly detected histochemically.
008349 B6;129-Ptger3tm1Csml/J
Cryopreserved - Ready for recovery
021233 B6;129-Raf1tm2Ara/J
Cryopreserved - Ready for recovery
Mice homozygous for the Raf1loxP/loxP allele are viable and fertile. This allele contains a floxed sequence containing a cDNA fragment encoding v-raf-leukemia viral oncogene 1 (Raf1) wild-type exons 13 to 16 and a neomycin (neo) resistance cassette downstream of exon 12 of the Raf1 gene. A point mutation was introduced downstream of the floxed sequence, resulting in the missense mutation D486N, associated with the autosomal dominant disorder Noonan syndrome (NS). Raf1 is a MAP kinase kinase kinase (MAP3K), which is activated by Ras GTPases, which in turn activates MEK1/2 and extracellular signal-regulated kinase (ERK)1/2 pathways. Activated ERKs are involved in the cell division cycle, apoptosis, cell differentiation and cell migration. The D486N, kinase-impaired, mutation is associated with the autosomal dominant disorder Noonan syndrome (NS) characterized by short stature, facial dysmorphia, cardiovascular abnormalities, and myeloproliferative dise .....
For more information please see the full phenotype on the strain data sheet
007855 B6;129-Rims2tm1.2Schc/J
Cryopreserved - Ready for recovery
Mice homozygous for this floxed targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exon 5 is deleted by cre expression to produce a "RIM2alpha" isoform null allele. This strain may be helpful in the analysis of the function of alpha-RIMs in specific brain regions.
010673 B6;129-Runx1tm3.1Spe/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in the cre-expressing tissues.

When bred to a strain with inducible Cre recombinase during development (see Stock No. 003556 for example), this mutant mouse strain may be useful in studies of hematopoiesis and development.

004162 B6;129-Scaptm1Mbjg/J
Cryopreserved - Ready for recovery
Mice that are homozygous for this targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. They posses a loxP-flanked neomycin resistance cassette located 3 kb upstream of exon 1. A third loxP site is located in intron 1. When used in conjunction with a Cre recombinase-expressing strain, this strain is appropriate for generating tissue-specific knockout mutants of Scap. This mutant mouse strain represents a model that may be useful in studies related to cholesterol and fatty acid homeostasis.
008366 B6;129-Smad1tm1Abr/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 2 in the targeted gene. Mice that are homozygous for the floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. Homozygous knock out mice are embryonic lethal. Literature associated with this gene suggests the mice may be useful in studies of reproduction, development, the cardiovascular system and cancer.
006099 B6;129-Spi1tm1.2Dgt/J
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. Endogenous protein expression is unaffected by the loxP sequences flanking an upstream regulatory region (URE). When bred to mice with a cre recombinase gene under the control of a promoter of interest, the URE of the targeted gene is deleted in the tissue of interest. Deletion of this "floxed" URE may be useful in studying T cell lymphoma, AML and other cancers, as well as transcription factors, hematopoiesis, and development of multiple cell lineages.
012346 B6;129-Terf2iptm1.1Tdl/J
Cryopreserved - Ready for recovery
Mice homozygous for this Rap1F allele are viable and fertile, with loxP sites flanking exon 2 of the mouse Rap1 gene (also called mRap1, TRF2-interacting factor, Terf2ip, or telomeric repeat binding factor 2 interacting protein). When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the TRF2 binding domain deleted in the cre-expressing tissues. As Rap1 is one of the components of shelterin, these Rap1F mutant mice may be useful in generating conditional mutations for studying the shelterin complex of telomeres, telomere maintenance, chromosomal stability, cancer, and aging.
016574 B6;129-Tmem259tm1.1Itl/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 2 through 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 4 deleted in the cre-expressing tissues.
008678 B6;129-Ubbtm1Rrk/J
Cryopreserved - Ready for recovery
Mice heterozygous for the targeted allele are viable and fertile. This polyubiquitin B (Ubb) mutation is characterized by a GFP-puror fusion protein "knock-in" allele that also abolishes endogenous gene function. Direct visualization of GFP fluorescence is observed in ovaries, testes, hypothalamus (arcuate nucleus) and cerebral cortex. Homozygotes have no Ubb mRNA observed in the various tissues tested, and are viable but sterile due to failure of germ cells to progress through meiotic prophase I and hypogonadism. Homozygotes also exhibit a complex metabolic phenotype initially characterized by dysfunction of neurons within the central nervous system accompanied by retarded perinatal growth that progresses to adult-onset obesity linked to selective hypothalamic neurodegeneration. Homozygotes also develop adult-onset hyperleptinemia (but normal levels of circulating glucose and insulin) as a consequence of increased fat content. These Ubb-mutant mice may be useful in studyin .....
For more information please see the full phenotype on the strain data sheet
019518 B6;129-Vangl1tm1.1Nat/J
Cryopreserved - Ready for recovery
The third (and largest) coding exon of Vangl1 (vang-like 1 (van gogh, Drosophila)) is flanked by loxP sites in this conditional knockout allele. Crosses with mice expressing Cre recombinase, enable tissue-specific excision of the floxed segment, presumably eliminating Vangl1 function.

Although the role of this gene is still being elucidated, mutations in this locus have been associated with neural tube closure defects and left-right asymmetry.

017839 B6;129P-Fat3tm1.1Good/J
Cryopreserved - Ready for recovery
FAT3, an atypical cadherin protein, is expressed on amacrine cell (AC) processes in the developing and mature inner plexiform layer (IPL) of the retina and is associated with dendrite morphogenesis. These mutant mice possess loxP sites flanking exon 23 of the Fat3 gene. Mice that are homozygous for this allele are viable, fertile, and normal in size. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 23 deleted in the cre-expressing tissues. This strain may be useful for studying dendrite development.
007605 B6;129P-Psen1tm1Vln/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 7 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these "floxed" mice are bred to mice that express Cre recombinase, resulting offspring can have one of three resulting genotypes (only exon 7 deleted, only the neo selection cassette deleted, or both exon 7 and the neo selection cassette deleted) in the cre-expressing tissue(s). These PS1-floxed mice may be useful in generating conditional knockouts of Presenilin 1 for studying Alzheimer's Disease.

For example, when crossed to a strain expressing Cre recombinase in postnatal neurons (see Stock No. 006143), this mutant mouse strain may be useful in studies of amyloid plaque formation.

016913 B6;129P2-Gmnntm1Tjm/J
Cryopreserved - Ready for recovery
These mice possess loxP sites flanking exons 5-7 of the geminin (Gmnn) gene. Homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Geminin is a regulatory protein involved in cell division and differentiation in the central nervous system, the axial skeleton, and the eye. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 5-7, encoding the dimerization domain and Cdt1 (chromatin licensing and DNA replication factor 1) binding site, deleted in the cre-expressing tissue. For example, when crossed to mice expressing cre driven by the myxovirus (influenza virus) resistance 1 (Mx1) promoter (see Stock No. 003556 for example), resulting offspring will lack Geminin in bone marrow cells. In such mice, red blood cell and leukocyte production is decreased and megakaryocyte production .....
For more information please see the full phenotype on the strain data sheet
010523 B6;129P2-Gt(ROSA)26Sortm1(CAG-ALPP)Fawa/J
Cryopreserved - Ready for recovery
These mice contain "STOP-hPLAP", the human alkaline phosphatase, placental (Regan isozyme), ALPP, gene under the control of the CAG (chicken beta actin promoter/enhancer and cytomegalovirus immediate-early enhancer) promoter, inserted into the Gt(ROSA)26Sor locus. Expression of the ALPP gene is blocked by a loxP-flanked STOP fragment placed between the ALPP sequence and the Gt(ROSA)26Sor promoter. In the absence of Cre recombinase, no ALPP staining is detected in all tissues examined so far including the central and peripheral nervous system, skin, muscle, lung and heart. This strain serves as a reporter strain, with successful Cre-mediated excision being indicated by ALPP expression in cre-expressing tissues. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of cell lineage trac .....
For more information please see the full phenotype on the strain data sheet
012336 B6;129P2-Terf1tm2.1Tdl/J
Cryopreserved - Ready for recovery
These TRF1F mice harbor loxP sites flanking exon 1 (encoding the translation start site) of the telomeric repeat binding factor 1 locus. Telomeres serve a dual role in protecting the chromosome ends from degradation/repair activities and in intracellular signaling for regulating cell proliferation. Mammalian telomeres are formed by tandem TTAGGG sequence repeats bound by a specialized complex of six telomere-associated proteins called the shelterin complex. As TRF1 is one of the components of shelterin, these TRF1F mutant mice may be useful in generating conditional mutations for studying the shelterin complex of telomeres, telomere maintenance, chromosomal stability, cancer, and aging.
006568 B6;129P2-Terf2tm1Tdl/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 1 and 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele that can result in acute telomere dysfunction.

For example, when crossed to a strain expressing interferon inducible Cre recombinase (see Stock No. 003556), this mutant mouse strain may be useful in studies of telomere damage response.

022516 B6;129S-Gt(ROSA)26Sortm1(Cdkn1c)Jfpa/J
Cryopreserved - Ready for recovery
The R26loxpTA-p57 allele has a loxP-flanked transcriptional termination cassette upstream of a mouse p57Kip2 (Cdkn1c) cDNA sequence, all inserted into the Gt(ROSA)26Sor locus. Although under control of the Gt(ROSA)26Sor promoter, widespread expression of p57Kip2 is prevented by the upstream floxed-STOP cassette in the absence of Cre recombinase. Heterozygous mice (R26loxpTA-p57k/+) are viable and fertile with no reported phenotypic abnormalities. The donating investigator reports that homozygous mice (R26loxpTA-p57k/k) have the same phenotype as heterozygous mice.

When bred to mice that express Cre recombinase, offspring will have the floxed-STOP cassette deleted in cre-expressing cells; resulting in p57Kip2 expression and subsequent cell cycle inhibition/arrest in G1 phase.

For example, when R26loxpTA-p57 mice are bred with mice expressing Cre recombinas .....
For more information please see the full phenotype on the strain data sheet

003902 B6;129S-Mttptm2Sgy/J
Cryopreserved - Ready for recovery
These mice possess loxP sites located 2.5 kb 5' of exon 1 and flanking a neomycin resistance gene inserted into intron 1 of the Mttp gene. Mice that are homozygous for this floxed Mttp allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
When bred to a strain expressing interferon inducible Cre recombinase in liver tissue (see Stock No. 003556 for example), this mutant mouse strain may be useful in lipoprotein research.
014181 B6;129S-Prkd1tm1Eno/J
Cryopreserved - Ready for recovery
These mice possess loxP sites flanking exons 12 through 14 of the targeted gene, which encode part of the catalytic domain. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 12 through 14 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in cardiomyocytes (see Stock No. 011038 for example), this mutant mouse strain may be useful in studies of cardiac response and remodeling due to pathological stress.

010686 B6;129S-Snai1tm2Grid/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 and 3 deleted in the cre-expressing tissue(s).
020288 B6;129S-Sptbn1tm1.1Mnr/J
Cryopreserved - Ready for recovery
Spnb2fl/fl mutant mice possess loxP sites flanking exon 3 of the spectrin beta, non-erythrocytic 1 (Sptbn1) gene. βII-spectrin is a molecular scaffold protein located in the distal regions of the axon where it links the plasma membrane to the actin cytoskeleton. βII-spectrin plays a role in neuron axonal polarity and growth by linking axon initial segments (AISs) to the cytoskeleton. Homozygotes are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues. Widespread deletion results in embryonic lethality.

When bred to B6.Cg-Tg(Nes-cre)1Kln/J mice (Stock No. 003771) with neuronal cre-expression, resulting mice exhibit significant fragmentation and disruption of AIS in the hippocampus and cortex.

012901 B6;129S-Stat1tm1Mam/Mmjax
Cryopreserved - Ready for recovery
Mice homozygous for the targeted mutation are viable and fertile and exhibit no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific conditional mutants of the floxed gene. This mutant mouse strain may be useful in generating conditional mutations to study autonomous roles of STAT1 in ERBB2/neu induced mammary tumorigenesis.

Heterozygote: Not evaluated

007032 B6;129S-Wnt4tm1.1Bhr/BhrEiJ
Cryopreserved - Ready for recovery
This strain contains loxP sites flanking exon 2 of Wnt4 resulting in a Cre-dependent conditional null allele. Homozygotes are normal. Studies by Kobayashi et al., determined that when this conditional allele is exposed to Cre expression by Amhr2tm3(cre)Bhr Mullerian duct regression proceeds normally.
009389 B6;129S1-Bambitm1Jian/J
Cryopreserved - Ready for recovery
Mice homozygous for this Bambiflox allele are viable and fertile, with loxP sites flanking exon 1 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the endogenous signal peptide deleted in the cre-expressing tissue(s); this is expected to produce a null allele. These mutant mice may be useful in generating conditional mutations for studying the role of Bambi in developmental biology and the TGF-beta pathway.
017956 B6;129S1-Glstm1Sray/J
Cryopreserved - Ready for recovery
Glutaminase is involved in glutamate neurotransmitter recycling, nitrogen metabolism, glycolysis in the presence of oxygen (Warburg effect) and regulation of reactive oxygen species. These mice carry a floxed STOP cassette that disrupts exon 1, and when bred to mice that express Cre recombinase, resulting offspring will exhibit expression of Gls in cre-expressing tissues. In the absence of recombination, the targeted mutation is a null allele and homozygotes die within the first postnatal day due to respiratory distress. Homozygotes are slightly smaller at birth than controls. Mice that are heterozygous for the targeted mutation are viable and fertile. No gene product (mRNA or protein) is detected by QRT-PCR analysis of fetal and adult tissue and Western blot analysis of brain tissue. No enzymatic activity is detected in neonatal brain, kidney, and liver tissues from homozygotes. Although neonate homozygotes are able to suckle, they do not feed and exhibit b .....
For more information please see the full phenotype on the strain data sheet
017894 B6;129S1-Glstm2Sray/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of the Gls exon 1, bracketing the start codon. Glutaminase is involved in glutamate neurotransmitter recycling, nitrogen metabolism, glycolysis in the presence of oxygen (Warburg effect), and regulation of reactive oxygen species. Mice that are homozygous for this allele are viable, fertile and normal in size. When bred to mice that express Cre recombinase, the resulting offspring will have exon 1 and the start codon deleted in the cre-expressing tissue(s).

When used in conjunction with a Cre recombinase-expressing strain, this mutant mouse strain may be useful in studies related to glutamate neurotransmitter recycling, metabolic compensation in the kidney and anaerobic cancer cell metabolism.

021980 B6;129S1-Nodaltm2Mku/J
Cryopreserved - Ready for recovery
These Nodalfl2 floxed mutant mice possess loxP sites flanking exons 2-3 of the Nodal gene. NODAL belongs to the Transforming Growth Factor beta (TGF-β) superfamily of secreted signaling molecules and plays a role in axial patterning and germ layer development during early vertebrate development. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2-3 deleted in cre-expressing tissues.

For example, when crossed to a strain expressing Cre recombinase in the mesodermal and definitive endodermal lineages, double mutant mice exhibit left/right axial defects.

004596 B6;129S1-Smarcb1tm3Sho/J
Cryopreserved - Ready for recovery
This strain contains loxP sites, in opposing orientation, flanking the targeted gene resulting in an reversible Cre-mediated conditional null allele. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with an inducible Cre recombinase-expressing strain, this strain is useful in generating mutants of the floxed allele that exhibit partial penetrance. In the presence of high levels of Cre protein, the orientation of the floxed allele is continually in a reversible reaction. All progeny resulting from a cross of these mice with Mx-Cre mice developed T-cell lymphoma or rhabdoid tumors at a median onset of age 11 weeks. The rhaboid tumors exhibited in these progeny mice closely resemble human malignant rhaboid tumors.
017359 B6;129S2-Cxadrtm1.1Ics/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 2 of the coxsackie virus and adenovirus receptor gene (Cxadr), a cell adhesion molecule critical in early embryogenesis. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

When bred to a strain with widespread cre/Esr1 expression (Stock No. 00 4682), this mutant mouse strain may be useful in studies of cardiac, gastrointestinal, pancreatic, and thymic development and physiology.

016230 B6;129S4-Bmp2tm1Jfm/J
Cryopreserved - Ready for recovery
Mice homozygous for this Bmp2floxneo allele have loxP sites flanking exon 3 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the endogenous mature ligand deleted in the cre-expressing tissue(s) resulting in a null allele. Homozygous mice are viable and fertile. These mutant mice may be useful in generating conditional mutations for studying the role of Bmp2 in bone morphogenetic protein signaling pathways, bone biology, cardiovascular biology, and cancer (Bmp2 misregulation is associated with cancer cell motility/invasiveness and epithelial-to-mesenchymal transition/transformation [EMT]).
017568 B6;129S4-E4f1tm1.1Llca/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 4 through 14, of the targeted gene. E4f1 is a multifunctional transcription factor with widespread expression and is involved in signaling pathways that regulate cell proliferation and survival, and tumorigenesis. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 4 through 14 deleted in the cre-expressing tissue(s).
003309 B6;129S4-Gt(ROSA)26Sortm1Sor/J
Cryopreserved - Ready for recovery
Mice heterozygous or homozygous for the Gtrosa26tm1Sor targeted mutation may be used to test the tissue/cellular expression pattern of the cre transgene in any transgenic strain carrying cre under the regulation of a specific promoter. Cre expression results in the removal of a loxP-flanked DNA segment that prevents expression of a lacZ gene. When crossed with a cre transgenic strain, lacZ is expressed in cells/tissues where cre is expressed.
009046 B6;129S4-Hrastm1Tyj/J
Cryopreserved - Ready for recovery
A G12V mutant form of protein is conditionally expressed from this gene when a floxed stop segment is excised by Cre. This oncogene is involved with the development of Costello syndrome, a neuro-cardio-facio-cutaneous developmental syndrome.
012356 B6;129S4-Pbx3tm1Og/J
Cryopreserved - Ready for recovery
These Pbx3C mutant mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s). The donating investigator reports that the neo cassette is still present downstream of the floxed exon and it is unknown whether the presence of neo conveys any abnormalities.
012679 B6;129S4-Ptpn1tm2Bbk/Mmjax
Cryopreserved - Ready for recovery
Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in generating conditional mutations to study energy regulation, adiposity, glucose homeostatsis and leptin sensitivity.

For example, when bred to mice carrying Tg(Alb-cre)21Mgn,Cre recombinase expression is directed to the liver; this mutant mouse strain may be useful in studies of body weight regulation.

When bred to mice carrying Tg(Nes-cre)1Kln, Cre recombinase is directed to the central and peripheral nervous system; this mutant mouse strain may be useful in studies of the regulation of adiposity, leptin and body weight.

008177 B6;129S4-Rbl2tm2Tyj/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 2 of the retinoblastoma-like 2 (Rbl2) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to Cre transgenic strains, the loxP-flanked exon is excised to produce a null allele. Homozygous null mice made in this fashion show no expression of the protein coded by this gene and have no gross phenotype.
018353 B6;129S4-Ror1tm1.1Meg/J
Cryopreserved - Ready for recovery
These Ror1f/f mutant mice possess loxP sites flanking exons 3-4 of the receptor tyrosine kinase-like orphan receptor 1 (Ror1) gene. Mice that are homozygous for this allele are viable and fertile. ROR1 is a receptor for Wnt5a-mediated signaling. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 3-4 deleted in the cre-expressing tissues.

When bred to Tg(EIIa-cre)C5379Lmgd transgenic mice, ROR1 null mice are morphologically and phenotypically normal, although some exencephaly has been seen. When Ror2 gene expression is also abolished (see Stock No. 018354) these double KO mice exhibit decreased branching of the sympathetic neuron axons, facial malformations, and truncation of the limbs and posterior region. Double KO mice die by E14.5.

018020 B6;129S4-Srsf1tm1Xdfu/J
Cryopreserved - Ready for recovery
Srsf1 (serine/arginine-rich splicing factor 1) is a serine/arginine (SR)-rich protein associated with the regulation of consititive splicing.

These targeted mutation mice carry a floxed allele of the gene. When crossed with a cre strain, tissue specific excision of exons 2-4 can be accomplished.

Ubiquitous excision of the floxed segment is early embryonic lethal. Conditional knockout in cardiomyocytes with a Myl2-cre strain causes postnatal contraction defects and dilated cardiomyopathy.

When bred to mice carrying Tg(Chx10-EGFP/cre,-ALPP)2Clc (see Stock No. 005105 for example) Cre recombinase expression in retina and Muller glial cells, this mutant mouse strain may be useful in studies of embryonic retinal development.

018019 B6;129S4-Srsf2tm1Xdfu/J
Cryopreserved - Ready for recovery
Srsf2 (serine/arginine-rich splicing factor 2) is a serine/arginine (SR)-rich protein associated with the regulation of consititive splicing.

These targeted mutation mice carry a floxed allele of the gene. When crossed with a cre strain, tissue specific excision of the first two coding exons can be accomplished.

Ubiquitous excision of the floxed segment is embryonic lethal. Conditional knockout in thymus using an Lck-cre strain causes a defect in T cell maturation by altering alternative splicing of CD45. Disruption of expression in the heart with an Myl2-cre strain causes dilated cardiomyopathy.

008303 B6;129S4-Tcf3tm4Zhu/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of the exons of the targeted gene that encode the bHLH region of the E12 and E47 isoforms. Transcription stop sequence and sequence encoding IRES-beta-galactosidase was inserted downstream of the loxP site flanked sequence. This strain can serve as a reporter strain, with successful Cre excision being indicated by beta-galactosidase expression in Cre-expressing tissues. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the exons encoding the bHLH region of the E12 and E47 isoforms deleted and beta-galactosidase expressed in the cre-expressing tissue(s).
008361 B6;129S4-Trp53tm5Tyj/J
Cryopreserved - Ready for recovery
These targeted mutant mice carry a loxP-flanked STOP cassette in intron 1 that blocks expression of the gene. Both heterozygotes and homozygotes are prone to the development of tumors (primarily spontaneous thymic lymphomas and sarcomas). The latency is significantly shorter in homozygotes than in wildtype mice. Homozygotes have reduced fertility. Upon Cre-mediated recombination, the STOP cassette can be excised, leading to restoration of gene expression. This strain permits temporal control of this tumor suppressor gene, and enables studies of tumorigenesis.
014649 B6;129S4-Yy1tm2Yshi/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissue(s).

When crossed with mice that express Cre recombinase specifically in early B-cell progenitors, this mutant mouse strain may be useful in studies of B-cell maturation.

009357 B6;129S6-Adam10tm1Zhu/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissues.

When bred to a strain expressing Cre recombinase in T cells for example(see Stock No. 003802), this mutant mouse strain may be useful in studies related to thymocyte developmental defects.

018539 B6;129S6-Bpnt1tm2Yrk/J
Cryopreserved - Ready for recovery
Bpnt1 is (bisphosphate 3'-nucleotidase 1) is a magnesium-dependent phosphomonoesterase that can be inhibited by lithium, a drug used to treat manic depression.

Exons 4 and 5, and an FRT-neomycin-FRT cassette located in intron 5 are flanked by loxP sites in these Bpnt1 conditionally targeted mutation mice. The floxed mice do not show any overt phenotype and expression of BPNT1 is normal with no detectable defects in liver, kidney, brain and small intestine. When the floxed segment is excised by Cre recombinase, tissue-specific knockouts of the gene can be produced.

019144 B6;129S6-Dpf2tm1.2Grc/J
Cryopreserved - Ready for recovery
Dpf2 (D4, zinc and double PHD fingers family 2) is a gene that functions as a transcription factor involved with apoptosis, hematopoiesis, and cancer.

Exons 2 and 3 of this mutant mouse strain are flanked by loxP sites. Crosses with Cre strains enable tissue-specific excision of the floxed region to generate knockouts that do not express protein.

021608 B6;129S6-Gaktm2Legr/Mmjax
Cryopreserved - Ready for recovery
These Gakflox mutant mice possess loxP sites flanking exon 1 of the cyclin G associated kinase (Gak) gene. GAK, a cyclin-dependent protein kinase, is a multidomain protein that functions as a cochaperone with Hspa8 (Hsc70). GAK is involved in the process of clathrin-mediated endocytosis and is critical to development. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have a null allele in the cre-expressing tissues.

For example, when bred to mice carrying Tg(Nes-Cre)1Kln, neuronal Cre recombinase expression results in early lethality and a failure of neuroprogenitor cells to proliferate.

When bred to mice carrying Tg(Alb-cre)21Mgn, Cre recombinase expression in the liver results in lethality by day 5, jaundice, disorganized bile ducts, undifferentiated hepatoblasts, fragmented Golgi and failure to accumulate glycogen. .....
For more information please see the full phenotype on the strain data sheet

016999 B6;129S6-Gt(ROSA)26Sortm1(xstpx-rtTA2S*M2)Whsu/J
Cryopreserved - Ready for recovery
These mice contain the rtTAS*M2, or rtTA2S-M2, an optimized form of reverse tetracycline regulated transactivator, gene inserted into the Gt(ROSA)26Sor locus. Expression of the rtTAS*M2 is blocked by a loxP-flanked STOP fragment placed between the rtTAS*M2 sequence and the Gt(ROSA)26Sor promoter. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will express rtTAS*M2, reverse tetracycline regulated transactivator, in the cre expressing cells. When bred to mice that also carry a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the gene of interest may be regulated by the tetracycline analog, doxycycline (dox). In the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not disp .....
For more information please see the full phenotype on the strain data sheet
020100 B6;129S6-Mir193btm1.1Lgp/J
Cryopreserved - Ready for recovery
MiR-193bf/f mutant mice possess loxP sites flanking the entire microRNA 193b (miR-193b) coding region. MiR-193b is a microRNA involved in post-transcriptional regulation of genes expressed during brown adipose tissue (BAT) differentiation. Homozygous mice are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have miR-193b in the cre-expressing tissues.

For example, when bred to STOCK Tg(MMTV-cre)4Mam/J mice (Stock No. 003553) with widespread expression of Cre recombinase, these mice showed no overt phenotype, perhaps due to the compensatory expression of mir-133a1 and mir-133a2 alleles.

008293 B6;129S6-Pclotm2Sud/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 14 and an Frt-flanked neomycin cassette knocked into intron 14 of the piccolo (presynaptic cytomatrix protein) gene. Homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing mouse, this strain is useful in creating a knockout line (see Stock No. 006391). No overt phenotype has been observed in this strain.
012284 B6;129S7-Dicer1tm1Smr/J
Cryopreserved - Ready for recovery
These mice carry a floxed allele of Dicer1 (Dicer1, Dcr-1 homolog (Drosophila)).When bred to mice expressing cre recombinase, exons 22 and 23 (encoding the RNase III domain) are deleted in the cre-expressing tissues of the offspring, resulting in a non-functional allele. This strain may be useful in studies of skin and hair follicle biology as well as mammalian organogenesis.
011039 B6;129S7-Map3k7tm1Mds/J
Cryopreserved - Ready for recovery
These mice carry a floxed allele of Map3k7 (mitogen-activated protein kinase kinase kinase 7). When bred to mice expressing cre recombinase, exon 1 of the targeted gene is deleted in the cre-expressing tissues of the offspring. This strain may be useful in studies of Wolff-Parkinson-White syndrome, electrophysiological and biochemical properties of the heart.

For example, when crossed to a strain expressing Cre recombinase in cardiac muscle cells (see Stock No. 011038), this mutant mouse strain may be useful in studies of cell metabolism.

012685 B6;129S7-Mstntm1Swel/J
Cryopreserved - Ready for recovery
Exon 3 of the Mstn (myostatin) gene is flanked by loxP sites in this targeted mutation strain. Homozygotes for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in studies of muscle growth and maintenance.

For example, when crossed to a strain expressing tamoxifen-inducible Cre recombinase in most tissues (see Stock No. 004682), this mutant mouse strain may be useful in studies of muscle development.

012436 B6;129S7-Tg(CAG-lacZ,-BMPR1A*,-EGFP)1Mis/Mmjax
Cryopreserved - Ready for recovery
caBmpr1a transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice possess a floxed Tau-β-galactosidase gene followed by a constitutively active mutant form of human BMP receptor type IA (caBmpr1a Q227D), an IRES sequence, and the EGFP coding sequence. Transgenic mice have widespread expression of lacZ prior to exposure to Cre recombinase. When bred with mice that express Cre recombinase, offspring will have the floxed-lacZ gene deleted and subsequent expression of caBmpr1a and EGFP in the cre-expressing cells. These mice may be useful for studying BMP signaling in tissues where Cre expresses such as cartilage and muscle, osteoclastogenesis (via RANKL-OPG pathway and/or canonical Wnt signaling), age dependant bone loss, osteoporosis, chondrodysplasia, fibrodysplasia ossificans progressive, and mineralization in muscle/skeletal development.
009363 B6;129X1-Cdc25atm1Hpw/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 1-3 in the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissues. (widespread deletion of expression is embryonic lethal). This strain may be useful in studies of development, cell cycles, and cancer in adult mice.
011129 B6;129X1-Cdc25btm1Hpw/J
Cryopreserved - Ready for recovery
These mice carry a floxed allele of Cdc25b (cell division cycle 25 homolog B (S. pombe)). When bred to mice with a cre recombinase gene, exons 2-14 of the targeted gene are deleted in the cre expressing tissues of the offspring. This strain may be useful in studies of cell division and oocyte maturation.
021296 B6;129X1-Kmt2atm2Sjk/JjdhJ
Cryopreserved - Ready for recovery
These Mll-CbpSTOP mice carry a floxed STOP cassette upstream of exon 8 of the myeloid/lymphoid or mixed-lineage leukemia 1 (Mll1) gene, and a CREB binding protein (Cbp) cDNA, a Simian virus 40 polyadenylation signal (SV40 pA), and a puromycin resistance sequence (puro) in Mll1 exon 8. Mice that are heterozygous for the targeted mutation are viable and fertile with no Mll-Cbp fusion protein expression detected. Homozygous mice are not viable. The MLL-CBP fusion protein is a characteristic of the t(11;16)(q23;p13) chromosomal translocation found in patients with therapy-induced myeloproliferative diseases, including acute myelomonocytic leukemia (t-AMML), chronic myelomonocytic leukemia (t-CMML), and myelodysplastic syndrome (t-MDS).

When bred to mice that express interferon-inducible Cre recombinase (see Stock No. 003556), resulting offspring treated with polyinosinic-pol .....
For more information please see the full phenotype on the strain data sheet

012388 B6;129X1-Ppargc1btm1Dpk/J
Cryopreserved - Ready for recovery
These mice carry a floxed allele of Ppargc1b (peroxisome proliferative activated receptor, gamma, coactivator 1 beta). When bred to mice with a cre recombinase gene under the control of a promoter of interest, exons 4-6 of the targeted gene are deleted in the tissue of interest. This strain may be useful in studies of the metabolic and functional maturation in the tissue of interest, using a conditional "knockout" strategy.
008467 B6;129X1-Wnt7btm2Amc/J
Cryopreserved - Ready for recovery
Mice homozygous for the Wnt7bc3 allele are viable and fertile, with loxP sites flanking exon 3 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). Unlike other Wnt7b mutant alleles, this Wnt7bc3 conditional allele is not affected by alternative exon 1 splicing. These Wnt7bc3 mice may be useful in generating conditional mutations for studying the role of Wnt7b (and other Wnt family members) in development and canonical Wnt signaling cascades, including lung differentiation and growth. In addition, these mice may also be useful in conjunction with other Wnt7 mutant strains including Wnt7b knockout mice (Stock No. 004693) and Wnt7a mutant mice (Stock No. 004715).

When bred to a strain expressing Cre recom .....
For more information please see the full phenotype on the strain data sheet

017954 B6;C-Tg(CAG-Epha4/Efna5,-mCherry)1Slp/J
Cryopreserved - Ready for recovery
Cell-to-cell interactions of membrane-bound Eph receptor tyrosine kinases (RTKs) and ephrin ligands regulate a variety of developmental processes, among them motor axon navigation, interstitial branch formation, synapse formation, cell migration, vascularization and hindbrain segmentation.

These transgenic mice conditionally coexpress a FLAG-tagged EphA4 (Eph receptor A4) extracellular domain (ECD) fused to the glycosylphosphatidylinositol (GPI) anchor sequence of Efna5 (ephrin A5) under the control of a chicken beta actin/CMV (CAG) promoter. Until crossed with a cre strain, a floxed stop cassette blocks expression of the chimeric EphA4ECD-ephrin-A5GPI protein and monomeric cherry (mCherry) fluorescent protein. Both hemizygotes and homozygotes are viable and fertile.

Cre-directed removal of a floxed-STOP cassette activates expression of the chimeric protein, allowing tissue-specific silencing of ephrin-A-mediated "reverse" signalling. C .....
For more information please see the full phenotype on the strain data sheet

008605 B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J
Cryopreserved - Ready for recovery
Hemizygous IRG transgenic mice are viable and fertile, with widespread expression of a loxP-flanked optimized red fluorescent protein variant (DsRed-Express) directed to embryonic and adult tissues by the CAG promoter prior to exposure to Cre recombinase. When bred to cre-expressing mice, the resulting offspring have the DsRed-Express cassette deleted in the cre-expressing tissue(s), allowing expression of the enhanced green fluorescent protein (EGFP) cassette located just downstream. These IRG transgenic mice are a double-fluorescent, Cre-reporter strain; with widespread expression of red fluorescence prior to Cre recombinase exposure, and green fluorescence following cre-mediated recombination in a pattern determined by cre expression, and should provide a versatile tool for analyzing complex cellular relationships in a wide variety of tissues.

For example, when using IRG transgenic mice along with Nestin-Cre mice (see Stock No. For more information please see the full phenotype on the strain data sheet

006465 B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J
Cryopreserved - Ready for recovery
These ZW transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread, but mosaic, throughout the central and peripheral nervous systems. Purkinje cells display intense beta-galactosidase activity. Approximately 50% of the total neuron population express the transgene, as detected by beta-galactosidase activity. Newborn mice exhibit widespread beta-galactosidase activity. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with wheat germ agglutinin (plant lectin) expression in tissues expressing cre. The double reporter system makes it possible to distinguish a lack of reporter (lacZ) expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity at the individual cell level. This transgenic mouse strain may be useful in tracing transneuronal or trans-synaptic connections and circuits in brain regions or in the s .....
For more information please see the full phenotype on the strain data sheet
007041 B6Ei.129P2-Nr5a1tm2Klp/EiJ
Cryopreserved - Ready for recovery
Mice homozygous for this floxed allele are viable and fertile. These mutant mice have loxP sites flanking the C-terminal coding exon. When bred to Cre-recombinase expressing mice, offspring will have a deletion of this exon in the cre expressing tissue(s). These floxed mice may be useful in studying steroidogenic factors and pituitary gonadotrope function.

For example, when crossed to a strain expressing Cre recombinase in the anterior and intermediate lobes of the pituitary gland (see Stock No. 004426), this mutant mouse strain may be useful in studies of pituitary gonadotrope function.

017704 B6N.129-Pik3catm1Jjz/J
Cryopreserved - Ready for recovery
These p110αflox (p110alphaflox) mice possess loxP sites flanking exon 1 of the phosphatidylinositol 3-kinase, catalytic, alpha polypeptide (Pik3ca) gene. P110α is an isoform of the p110 catalytic subunit of PI3K, which is a critical enzyme in the insulin signaling pathway. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in cre-expressing tissues. For example, when crossed to a strain expressing Cre recombinase in the liver, this mutant mouse strain displays reduced insulin sensitivity, impaired glucose tolerance, and increased gluconeogenesis, hypolipidemia, and hyperleptinemia. They also exhibit a 34% reduction in serum free fatty acids, a 28% reduction in total serum cholesterol, and a 44% reduction in ser .....
For more information please see the full phenotype on the strain data sheet
012457 B6N.129-Ptch1tm1Hahn/J
Cryopreserved - Ready for recovery
These Ptchflox mutant mice possess loxP sites flanking exons 8-9 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 8-9 deleted in the cre-expressing tissue. The donating investigator reports that the frt-flanked neo cassette is still present downstream of the floxed exon that the presence of neo does not convey any abnormalities. This strain may be useful for studying Hedgehog/Patched signaling, cell-fate determination during embryogenesis, cell growth and differentiation, and development of T- and B-lymphoid lineages, hematopoietic stem cell diversification.
017705 B6N.129S(Cg)-Pik3cbtm1Jjz/J
Cryopreserved - Ready for recovery
These p110βflox mice possess loxP sites flanking exon 2 of the phosphatidylinositol 3-kinase, catalytic, beta polypeptide (Pik3cb) gene. P110β is an isoform of the p110 catalytic subunit of PI3K, which is a critical enzyme in the insulin signaling pathway. P110β, specifically, has been linked to tumor suppression in prostate tumor models. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in cre-expressing tissues. For example, when crossed to a strain expressing Cre recombinase in the liver, this mutant mouse strain displays impaired insulin sensitivity and glucose homeostasis. They have elevated levels of serum insulin and glucose, and they have impaired glucose tolerance and reduced insulin sensitivity. They show no chan .....
For more information please see the full phenotype on the strain data sheet
018502 B6N.129S1(Cg)-Lrrc7tm1.2Mabk/J
Cryopreserved - Ready for recovery
Densinflox mice possess loxP sites flanking exon 3, a region encoding the transcriptional start site of the leucine rich repeat containing 7 (Lrrc7) gene. Lrrc7 encodes the scaffolding protein densin, which is involved in cell adhesion and polarity. Densin is expressed in the postsynaptic density (PSD) of the central nervous system (CNS), with some expression also seen in glomerular podocytes and sertoli cells. Homozygous mice are viable, fertile, and normal in size. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in cre-expressing tissues.

When crossed to B6.FVB-Tg(EIIa-cre)C5379Lmgd/J mice (Stock No. 003724) with ubiquitous Cre recombinase expression, the surviving KO mice have deficits in short-term memory, spatial memory, prepulse inhibition, and nesting behavior similar to behaviors seen in mice with schizophreni .....
For more information please see the full phenotype on the strain data sheet

023347 B6N.129S1(FVB)-Ccl2tm1.2Tyos/J
Cryopreserved - Ready for recovery
These MCP-1flox mice possess loxP sites flanking exons 1-2 of the chemokine (C-C motif) ligand 2 (Ccl2) gene. MCP-1 (monocyte chemoattractant protein-1) is a chemotactic factor that recruits monocytes and basophils to sites of inflammation or injury. This chemokine plays a role in the development of inflammatory responses as well as some diseases including atherosclerosis, psoriasis, pulmonary fibrosis, nephritis, multiple sclerosis and cancer. Mice that are homozygous for this allele are viable and fertile. These mice produce MCP-1 whose level is compatible to that in WT mice in response to intraperitoneal injection of thioglycollate or zymosan. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 1-2 deleted in cre-expressing tissues.

For example, when crossed to B6.FVB-Tg(EIIa-cre)C5379Lmgd/J mice (Stock No. 003724) this mutant .....
For more information please see the full phenotype on the strain data sheet

012833 B6N.129S6-Myo1ftm1Flv/J
Cryopreserved - Ready for recovery
Exons 5 and 6 of these Myo1f (myosin IF) targeted mutation mice are flanked by loxP sites. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.
022505 B6N.Cg-Gpr116tm1.1Bstc/J
Cryopreserved - Ready for recovery
These Gpr116flox mutant mice possess loxP sites flanking exon 2, encoding the start codon, of the G protein-coupled receptor 116 (Gpr116). GPR116 is an endothelial cell specific transmembrane receptor. In addition to its pan endothelial expression Gpr116 is also expressed in lung Alveolar Type II cells where it plays a critical role in lung surfactant homeostasis. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

For example, when bred to B6.Cg-Tg(Tek-cre)12Flv/J mice (Stock No. 004128) expressing Cre recombinase in endothelial cells, resulting offspring display labored breathing by 4 months of age, weigh less than littermates by 14 months, and have a shortened lifespan. They also have an accumulation of surfactant proteins in th .....
For more information please see the full phenotype on the strain data sheet

016881 B6N.Cg-Gpr124tm1.1Bstc/J
Cryopreserved - Ready for recovery
These Gpr124flox mutant mice possess loxP sites flanking exon 1 of the G protein-coupled receptor 124 (Gpr124) targeted gene. Homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. GPR124 is overexpressed in tumor vasculature and is required for blood vessel migration, formation of the blood brain barrier, and expansion of the cerebral cortex. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissue. This strain may be useful for studying neurovasculature development and cerebrovascular disease.

When bred to mice carrying Tg(Tek-cre)12Flv (Stock No. 004128), Cre recombinase expression in the endothelial cells results in abnormal angiogenesis.

017297 B6N.Cg-Hmgn3tm1.1Mbus/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 3 and 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3 and 4 deleted in the cre-expressing tissue(s).
013042 B6N;129S-Acacbtm1.1Lowl/J
Cryopreserved - Ready for recovery
Mice homozygous for the Acc2flox allele are viable, fertile, normal in size, and have no reported physical abnormalities. The Acc2flox allele has loxP sites flanking the biotin-binding motif exon (and the preceding exon) of the targeted locus. When bred to mice that express Cre recombinase, the resulting offspring will have the floxed sequences deleted in the cre-expressing cells/tissues. The exon immediately upstream of the biotin-binding catalytic domain was included so that splicing of the remaining exons following Cre-mediated deletion would introduce a frameshift and nonsense mutation after Asp865 in any translated protein. These mutant mice may be useful to generate conditional mutations for studying malonyl-CoA (the substrate for fatty acid synthesis and the regulator of fatty acid oxidation) synthesis and other metabolic cellular signaling molecules, as well as diet-induced obesity, glucose intolerance and insulin resistance.

NOTE: W .....
For more information please see the full phenotype on the strain data sheet

019120 BALB/c-Gt(ROSA)26Sortm10(Lmp1)Rsky/J
Cryopreserved - Ready for recovery
These mice contain EBV latent membrane protein 1, Lmp1, coding sequence inserted into the Gt(ROSA)26Sor locus. LMP-1 (Epstein-Barr virus latent membrane protein 1) is a six-span transmembrane protein that is a functional, but dysregulated, mimic of CD40 signaling to B cells. Expression of the Lmp1 gene is blocked by a loxP-flanked STOP fragment placed between the Lmp1 sequence and the Gt(ROSA)26Sor promoter. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre expressing tissue resulting in expression of Lmp1. Mice that are homozygous for the targeted mutation are viable and fertile.

For example, when bred to mice carrying Cd19tm1(cre)Cgn (Stock No. 006785), Cre recombinase expression results in expression of LMP1 in B cells causing lymphoproliferation and lymphomagenesis.

007686 BKa.Cg-Sox17tm2Sjm Ptprcb Thy1a/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of the exon 3-5 region of the targeted gene. Mice homozygous for this allele are viable and fertile and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.

When the floxed allele is excised by crosses with a Tie2 (endothelial-specific receptor tyrosine kinase)-Cre mouse (Stock No. 004128) and the Sox17 targeted mutant EGFP reporter strain (Stock No. 007687), mutants are embryonic lethal around E13.5. Mutant embryos have severe hematopoietic failure and lack definitive hematopoietic stem cells.

When the floxed allele is excised by Mx1 (myxovirus (influenza virus) resistance 1)-Cre (Stock No. 003556) and the Sox17 .....
For more information please see the full phenotype on the strain data sheet

021023 C.129(B6)-Pkhd1tm2Cjwa/J
Cryopreserved - Ready for recovery
The PKHD1 (polycystic kidney and hepatic disease 1) gene or fibrocystin, is a large type I membrane protein associated with human autosomal recessive polycystic kidney disease (ARPKD). Pkhd1 is highly expressed in the kidney; the protein undergoes processing and is secreted by exosome-like vesicles (ELV) in the bile and urine. Pkhd1LSL mutant mice possess a loxP-STOP-loxP (LSL) cassette inserted into intron 2 and two SV5-Pk epitope tags placed in-frame into exon 3. The LSL cassette terminates all transcripts in intron 2, generating a null allele. Mice that are homozygous for Pkhd1LSL exhibit progressive polycystic liver disease and liver fibrosis visible by histology at one month of age. Females, but not males, develop proximal tubule dilation and cysts in the kidneys.

When crossed to mice carrying Tg(Gdf9-cre)5092Coo (Stock No. 011062), the LSL cassette is removed, the Pkhd1<> .....
For more information please see the full phenotype on the strain data sheet

009670 C.129P2(B6)-Gt(ROSA)26Sortm1(DTA)Lky/J
Cryopreserved - Ready for recovery
Homozygotes are viable, fertile and do not display any gross physical or behavioral abnormalities. When these ROSA-DTA mice are crossed with a Cre recombinase strain, the floxed-STOP cassette is deleted and the Gt(ROSA)26Sor promoter drives expression of diptheria toxin in the cre-expressing cells. These ROSA-DTA mice allow selective ablation in a tissue/cell-specific manner.

Of note, ROSA-DTA mice are also available on a C57BL/6 congenic background (see Stock No. 009669).

008603 C.129P2(B6)-Gt(ROSA)26Sortm1(tTA)Roos/J
Cryopreserved - Ready for recovery
Mice heterozygous for the ROSA:LNL:tTA conditional mutation of the Gt(ROSA)26Sor locus are viable and fertile, with a loxP-flanked STOP cassette preventing transcription of a downstream optimized/modified tetracycline-controlled transactivator protein ("mtTA"). Applying both Cre-lox and Tet-Off technologies, these ROSA:LNL:tTA mutant mice may be useful to generate compound mutant mice in which expression of a tetracycline-responsive promoter element (TRE; also called tet-operator or tetO)-driven gene of interest can be both directed to the cell types defined by the chosen Cre recombinase expression, as well as turned off by the addition of tetracycline (or its analog doxycycline (dox)).

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally describe .....
For more information please see the full phenotype on the strain data sheet

015859 C.129P2(Cg)-Il4/Il13tm1.1Lky/J
Cryopreserved - Ready for recovery
These Il4/Il13flox mutant mice possess a loxP site upstream of exon 3 of the interleukin 13 (Il13) gene and a second loxP site downstream of exon 4 of the interleukin 4 (Il4) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have nucleotides between exons 3 of Il13 and exon 4 of Il4 deleted in the cre-expressing tissue. This strain may be useful for studying immune function in mice lacking Il4 and Il13.
008879 C.B6-Arg1tm1Pmu/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 7 and 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 7 and 8 deleted in the cre-expressing tissue(s).

When bred to a strain with inducible Cre recombinase expression in the myeloid cell lineages (see Stock No. 004781, for example ) or endothelial cells (see Stock No. 004128 , for example), this mutant mouse strain may be useful in studies of immune response to bacterial and parasitic infections.

006575 C57BL/6-Camk2atm1Vyb/J
Cryopreserved - Ready for recovery
Mice homozygous for this "falpha-CaMKII" allele are viable and fertile with no reported neurological abnormalities. These mutant mice have loxP sites flanking exon 2 of the endogenous gene. When bred to Cre-recombinase expressing mice, exon 2 is deleted in the resulting offspring dependent on the tissue specificity of the promoter of the cre transgene.

For example, when crossed with transgenic mice expressing Cre-recombinase in hippocampal CA3 pyramidal cells (see Stock No. 006474), the resulting offspring show altered neurotransmitter release. These "floxed" mice may be useful for neurological studies such as calcium/calmodulin-dependent protein kinase activity.

016929 C57BL/6-Cd1d1tm1.1Aben/J
Cryopreserved - Ready for recovery
These mice carry a floxed allele of the Cd1d1 (CD1d1 antigen) gene. When bred to mice expressing Cre recombinase under the control of a chosen promoter, exons 2-6 are deleted in the tissue of interest, blocking expression of Cd1d1. As the homologous Cd1d2 gene has a frameshift mutation preventing expression of a functional protein, blocking expression of Cd1d1 results in the complete absence of CD1D proteins at the cell surface. Homozygous floxed mice are viable and fertile and do not display any gross physical or behavioral abnormalities.

When crossed with Cd4-Cre mice (see Stock No. 017336), progeny reportedly show an 8-fold reduction in natural killer T (NKT) cell numbers on average in a manner commensurate with the levels of CD1D protein downregulation achieved among cortical thymocytes. This result is consistent with the requirement for Cd1d1 expression by CD4+ CD8+ double posi .....
For more information please see the full phenotype on the strain data sheet

018135 C57BL/6-Cfl2tm1Itl/J
Cryopreserved - Ready for recovery
These Cofi/Cofi floxed mutant mice possess loxP sites flanking exons 2-4 of the cofilin 2 (Cfl2) gene. They also contain a frt-flanked neo cassette followed by a single loxP site downstream of exon 4. Cofilin is a member of the AC group of proteins expressed at sarcomeres in skeletal and cardiac muscles. Cofilin regulates muscle development and actin dynamics by severing actin filaments and causing actin depolymerization. Cfl2 mutations have been associated with the neuromuscular disorder 'nemaline myopathy with minicores', which is characterized by weakness and the presence of rodlike structures called nemaline bodies in affected muscles. Mice that are homozygous for this allele are viable and fertile. When these mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2-4 deleted in cre-expressing tissues.

For example, when crossed to B6.Cg-Tg(ACTA1-cre)79Jme/J mice (see Stock No. For more information please see the full phenotype on the strain data sheet

007895 C57BL/6-Fastm1Cgn/J
Cryopreserved - Ready for recovery
Mice homozygous for this "Fasfl" conditional allele are viable and fertile, with loxP sites flanking exon 9 of the targeted gene. When bred to mice that express Cre recombinase, exon 9 (which encodes the death domain) is deleted in the cre-expressing tissues in the resulting offspring.

These Fasfl mice may be useful in generating conditional mutations for studying many aspects of immune function. For example, when Fasfl mice are crossed to a strain expressing Cre recombinase in B lineage cells (see Stock No. 004126 or 006785 ), this mutant mouse strain may be useful in studies of lymphoproliferative disorder. Similarly, when Fasfl mice are crossed to an interferon inducible strain with widespread Cre recombinase expression (see Stock No. 003556, .....
For more information please see the full phenotype on the strain data sheet

012352 C57BL/6-Gt(ROSA)26Sortm8(Map2k1*,EGFP)Rsky/J
Cryopreserved - Ready for recovery
Mice homozygous for the R26StopFLMEK1DD conditional allele are viable and fertile, with a loxP-flanked Neo-STOP cassette preventing transcription of the downstream bicistronic sequences (MEK1DD [a mutant form of rat MAPKK1 rendered constitutively active by two serine->aspartic acid substitutions (S218D/S222D) within the catalytic domain] and EGFP). When bred to mice that express Cre recombinase, offspring will have the STOP cassette deleted and subsequent expression of the MEK1DD signal molecule and EGFP fluorescence in the cre-expressing cells. Expression of MEK1DD leads to constitutive activity of MAP Kinase signal transduction pathways that mediate various cellular activities, including gene expression, mitosis, differentiation, proliferation, cell survival, and cell cycle progression. Of note, breeding these mice to an FLP-expressing strain will result in removal of the frt-flanked IRES-EGFP cassette.
012361 C57BL/6-Gt(ROSA)26Sortm9(Rac1*,EGFP)Rsky/J
Cryopreserved - Ready for recovery
Mice homozygous for the R26StopFLRACDA conditional allele are viable and fertile, with a loxP-flanked Neo-STOP cassette preventing transcription of the downstream bicistronic sequences (RACDA [RacG12V; a mutant form of Rac1 rendered constitutively active by a glycine->valine substitution at amino acid 12] and EGFP). When bred to mice that express Cre recombinase, offspring will have the STOP cassette deleted and subsequent expression of the RACDA signal molecule and EGFP fluorescence in the cre-expressing cells. Expression of RACDA leads to constitutive activity of Rac1-dependent signal transduction; which is associated with gene expression, proliferation, cell survival, cell cycle progression, cytoskeletal reorganization, and Rho- and CDC42-pathways. Of note, breeding these mice to an FLP-expressing strain will result in removal of the frt-flanked IRES-EGFP cassette.
008304 C57BL/6-Nrastm1Tyj/J
Cryopreserved - Ready for recovery
This targeted mutant strain carries a loxP-flanked stop element in exon 1 and a G12D activating mutation in exon 2 of the neuroblastoma ras oncogene (Nras). This mutation functions as a null allele and has no apparent phenotype. Homozygotes are viable. Cre mediated excision of the floxed stop element causes a significant increase in GTP-bound N-Ras production. This conditional mutant strain may be helpful in further studies of this oncogene.

For example, when crossed to a strain expressing Cre recombinase in epiblast and ectoderm-derived tissues (see Stock No. 003755), this mutant mouse strain may be useful in studies of the role of NRAS in leukemogenesis.

When crossed to a strain expressing interferon-induced Cre recombinase (see Stock No. 003556), this mutant mouse strain may be useful in studies of leukemia.

017959 C57BL/6-Pmltm1(PML/RARA)Ley/J
Cryopreserved - Ready for recovery
The t(15;17)(q22:q11) translocation results in the PML-RARA, protein promyelocytic leukemia-retinoic acid receptor alpha, fusion gene, which is a characteristic chromosomal translocation in acute promyelocytic leukemia. These mPML-PRflox mice carry a loxP-flanked PGKneo-stop cassette, PML-RARA cDNA, in the 5' UTR of the Pml, promyelocytic leukemia, gene. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No Pml gene product (protein) is detected by Western blot analysis of spleen cells from homozygous animals. When bred to mice that express Cre recombinase, resulting offspring will express the fusion oncoprotein PML-PARA in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in the myeloid cell lineage (see Stock No. 004781 for example), the resulting m .....
For more information please see the full phenotype on the strain data sheet

006581 C57BL/6-Ppp3r1tm1Stl/J
Cryopreserved - Ready for recovery
Mice homozygous for this "fCNB1" mutant allele are viable and fertile with no reported neurological abnormalities. These mutant mice have loxP sites flanking exons 2-4 of the endogenous gene. When bred to transgenic mice expressing Cre recombinase, exons 2-4 are deleted in the resulting offspring in only those tissues expressing cre. For example, when crossed with transgenic mice expressing Cre recombinase specifically in the forebrain (similar to Stock No. 005359), the resulting offspring show abnormalities that are strikingly similar to those described for schizophrenia. These "floxed" mice may be useful in studies of calcineurin function in T cells (via NFAT transcription of cytokine genes) and in the central nervous system in, for example, neurite extension, synaptic plasticity, learning and memory, and schizophrenia pathogenesis.
010711 C57BL/6-Ptrh2tm1Eruo/J
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in generating conditional mutations to study anoikis /apoptosis.
021776 C57BL/6-Rab11fip5tm1Sud/J
Cryopreserved - Ready for recovery
Rab11Fip5 (RAB11 family interacting protein 5 (class I)) encodes a protein that interacts specifically with RAB11, which is key for endocytosis. In particular, Rab11Fip5 is required for transport in apical membranes. Preliminary investigations suggest a potential role for this gene in synaptic terminal exocytosis-endocytosis events and plasticity with potential relevance to autism.

In this conditional mutant strain, exon 2 of the Rab11fip5 gene is flanked by loxP sites. Mice homozygous for this allele are viable and fertile and produce normal levels of RAB11FIP5 protein. When crossed with a Cre-expressing strain or infected with Cre-expressing virus, the floxed allele is excised and expression of the mRNA is abolished.

010540 C57BL/6-Rev3ltm1Rsky/J
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Excision of the floxed fragment in B cells results in chromosomal instability, impaired B cell proliferation, reduced class switch recombination, reduced frequency of somatic mutations and increased frequency of DNA breaks. This mutant mouse strain is useful in studies of genome instability, DNA break repair and class switch recombination.

For example, when crossed to a strain expressing Cre recombinase in Mature B cells (see Stock No. 006368), this mutant mouse strain may be useful in studies of class switch recombination and DNA break repair.

017720 C57BL/6-Sh2d3ctm1Ebp/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 7 of the targeted gene. Exon 7 encodes most of the SH2 domain, and corresponds to exon 8 of human SHEP1. SH2D3C protein forms a complex with CAS (or BCAR1), a scaffolding protein, and regulates cell adhesion and migration. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 7 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in B-lymphocytes (see Stock No. 006785 for example), this mutant mouse strain may be useful in studies of marginal zone B-cell maturation, adhesion and migration.

When bred to a strain with germline Cre recombinase expression, this mutant mouse strain may be useful in studies of axona .....
For more information please see the full phenotype on the strain data sheet

006662 C57BL/6-Tg(ACTB-MAP2K1*K97M)1Stl/J
Cryopreserved - Ready for recovery
Hemizygous mice are viable and fertile. These "dnMEK1" mice express a dominant-negative mutant (K97M) form of human MEK1 (synonym: MAP2K1) following Cre-mediated removal of the upstream "Lox-STOP-Lox" cassette; when transgenic mice are bred to a cre-expressing strain, the "floxed stop" cassete is excised in the resulting offspring, and mutant MEK1 expression is observed in the cre-expressing tissue(s). In the absence of Cre recombinase, transgene expression is not detectable in the brains of these "floxed" mice Because the MEK1 mutation abolishes the protein's kinase activity but preserves its ability to interact with ERK1 and ERK2, these transgenic mice may be useful in studying MEK-dependent activation and regulation of ERK, the ERK-MAPK signaling pathway, and neurological studies involving synaptic plasticity and memory.

When bred to a strain expressing Cre recombinase in the CA1 pyramidal cell layer of the hippocampus (see Stock No. For more information please see the full phenotype on the strain data sheet

010712 C57BL/6-Tg(Camk2a-tTA)1Stl/J
Cryopreserved - Ready for recovery
When these transgenic mice are crossed with a tissue or cell-specific cre strain to remove a floxed stop cassette, tetracycline-controlled transactivator (tTA) is expressed in hippocampal CA3 and the dentate gyrus under the control of the calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter.

When further mated to a transgenic strain that carries a gene of interest driven by a tetracycline-responsive promoter element (TRE; tetO), expression of that gene can be conditionally regulated by the presence or absence of doxycycline in the drinking water. Expression in tTA/tetO animals can be reversibly inhibited by the presence of doxycycline or induced by withdrawal of the tetracycline analog.

006481 C57BL/6J-Tg(ACTB-NOTCH1)1Shn/J
Cryopreserved - Ready for recovery
Transgenic mice are viable, fertile and behaviorally normal. These "CALSL-NICD (H)" mice (or simply CALSL-NICD) reportedly carry 10-20 copies of the transgene inserted into a single genomic locus. Expression of the transgene-derived intracellular domain of human NOTCH1 is prevented by a "Lox-STOP-Lox" cassette. When transgenic mice are bred to a strain expressing Cre recombinase, the "floxed stop" cassette is excised in the resulting offspring, and human NOTCH1 expression is observed in the cre-expressing tissue(s). These transgenic mice may be useful in studying early neural progenitor cell development and apoptosis, and responses to tissue-specific Notch activation.

For example, when crossed to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771), this transgenic mouse strain may be useful in studies of notch signaling during apoptotic cell death.

016211 C57BL/6N-Agtr1atm1Uky/J
Cryopreserved - Ready for recovery
These AT1aflox mutant mice possess a loxP site upstream of exon 3 followed by a neomycin resistance (neo) cassette flanked by frt sites and loxP sites downstream of exon 3 of the angiotensin II receptor, type 1a (Agtr1a) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. AGTR1A is expressed in vascular cells such as endothelial cells, smooth muscle cells, and macrophages. Angiotensin II (Ang II) is a vasoconstrictor which, upon binding to AGTR1A, can induce aneurysms in the ascending aorta. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues. When this floxed strain is crossed to a strain expressing Cre recombinase in smooth muscle, deletion of Agtr1a had no affect on ascending aortic aneurysms (AA) after Ang II infusion. In contrast, when cr .....
For more information please see the full phenotype on the strain data sheet
013210 C57BL/6N-H3f3btm1Psk/Mmjax
Cryopreserved - Ready for recovery
These H3f3bFL mutant mice possess loxP sites flanking exons 2-4 of the H3 histone, family 3b (H3f3b) gene. The basic nuclear protein, H3F3B, is one of the four core histones responsible for the nucleosome structure. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in studies of chromosomal structure and function.

For example, when bred to mice carrying Tg(Zp3-cre)3Mrt, Cre recombinase expression in the oocyte results in reduced number of homozygotes, infertility, defective cell division and chromosome segregation.

015823 C57BL/6N-Pawrtm1Rang/J
Cryopreserved - Ready for recovery
Mice homozygous for this Par4flox allele are viable and fertile, with loxP sites flanking exon 2 of the targeted PRKC, apoptosis, WT1, regulator (Pawr or Par-4) gene. Homozygotes are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. PAR4 is a pro-apoptopic protein capable of inducing apoptosis in cancer cells and causing regression of tumors in animal models. PAR4 interacts with, and inhibit, atypical protein kinase C isoforms, functioning as a negative regulator of the NF-κB pathway. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 2 deleted in the cre-expressing tissue, resulting in inactivation of Pawr gene function. Loss of PAR4 leads to a reduction in apoptosis by increased activation of NF-κB. These mutant mice may be useful in generating conditional mutations for studying tumor development and treatment. .....
For more information please see the full phenotype on the strain data sheet
004081 C;129S-Vhltm1Jae/J
Cryopreserved - Ready for recovery
This strain contains loxP sites flanking the Vhl promoter and exon 1 resulting in a conditional null allele. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cre-mediated recombination results in the deletion of the promoter and exon 1. Studies in which liver-specific inactivation of the Vhl gene was achieved by breeding this strain with albumin promoter driven-Cre mice (see Stock No. 003574 for example) resulted in hemizygous mice that exhibit cavernous hemangiomas of the liver, a rare component of the human von Hippel-Lindau (VHL) disease. This strain represents an effective tool for generating tissue specific-targeted mutants useful in studies examining VHL and tumor suppression in general.

When bred to a strain expressing Cre recombinase in the myeloid cell lineage (see Stock No. For more information please see the full phenotype on the strain data sheet

008081 CBy.129(B6)-Sirt1tm1Ygu/J
Cryopreserved - Ready for recovery
Mice homozygous for this targeted allele (SirT1co/co) are viable and fertile with a loxP-flanked neomycin cassette just upstream of, and a third loxP site just downstream of exon 4 of the targeted gene. The floxed mutation does not affect the protein expression of the targeted gene in MEF's or mammary tissue from homozygotes. When bred to mice that express Cre recombinase, the resulting offspring have exon 4 (encoding a conserved Sir2 motif) deleted in the cre-expressing tissue(s) (the donating investigator reports that they observe only one recombination event; complete removal of the neomycin cassette and exon 4, leaving a single loxp site remaining). These SirT1co/co mice may be useful in generating conditional mutations for studying the role of estrogen, insulin growth factor-1 (IGF-1), and transcription factors (including NF-kappaB) in mammary gland development, breast cancer, apoptosis, and metabolic diseases.

In an att .....
For more information please see the full phenotype on the strain data sheet

009108 CBy.129P2(B6)-Myd88tm1Defr/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s).

When bred to a strain with inducible Cre recombinase expression in dendritic cells (see Stock No. 008068 for example), this mutant mouse strain may be useful in studies of Toll-like receptor signaling during immune responses.

When bred to a strain with Cre recombinase expression in hematopoietic cells (see Stock No. 008610 for example), this mutant mouse strain may be useful in studies of Toll-like receptor signaling and natural killer cells.

When bred to a strain with Cre reco .....
For more information please see the full phenotype on the strain data sheet

008040 CBy.B6-Gt(ROSA)26Sortm1(HBEGF)Awai/J
Cryopreserved - Ready for recovery
Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.

For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation.

When crossed to a strain expressing Cre recombinase in the pituitary and, at lower levels, in the testes (see Stock No. > .....
For more information please see the full phenotype on the strain data sheet

008338 CByJ.B6(Cg)-Rag2tm1Cgn/J
Cryopreserved - Ready for recovery
Mice homozygous for the RAG-2fl allele are viable and fertile, with loxP sites flanking exon 3 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for the entire RAG-2 protein) deleted in the cre-expressing tissue(s). These RAG-2fl mice may be useful in generating conditional mutations for studying the role of RAG-2 in B and T cell development (including cancer and toxicology research as a xenograft/transplant host), T and B cell receptor (V(D)J) recombination, hematopoiesis, hematology, immunology, and inflammation research.

For example, when bred to a strain with inducible Cre recombinase expression in liver and lymphocytes (see Stock No. 003556), this mutant mouse strain may be useful in studies of B and T cell development.

In an attempt to offer alleles on well-characterized or multiple genetic background .....
For more information please see the full phenotype on the strain data sheet

007042 D2.129P2(B6)-Nr5a1tm2Klp/EiJ
Cryopreserved - Ready for recovery
Mice homozygous for this floxed allele are viable and fertile. These mutant mice have loxP sites flanking the C-terminal coding exon. When bred to Cre-recombinase expressing mice, offspring will have a deletion of this exon in the cre expressing tissue(s). These floxed mice may be useful in studying steroidogenic factors and pituitary gonadotrope function.

For example, when crossed to a strain expressing Cre recombinase in the anterior and intermediate lobes of the pituitary gland (see Stock No. 004426), this mutant mouse strain may be useful in studies of pituitary gonadotrope function.

002981 DBA/2-Tg(xstpx-lacZ)36And/J
Cryopreserved - Ready for recovery
This test strain is used determine the tissue expression pattern of cre transgenic mice. The transgene is loxP-STOP-of-translation-loxP-lacZ driven by the beta-actin promoter. The lacZ is expressed only in cells where the STOP element has been removed by Cre recombinase. LacZ expression is restricted to neural and skeletal muscle tissue and heart by the chicken beta-actin promoter that is driving the reporter.
018543 FVB-Tg(CAG-cat,-Twist1)1Dbsp/J
Cryopreserved - Ready for recovery
The TWIST1 basic helix loop helix transcription factor, plays a role in heart and bone development, as well as lineage specification, differentiation and tumorigenesis. Mutations of TWIST1 in humans are associated with Saethre-Chotzen syndrome and Robinow-Sorauf syndrome. When these CAG-CAT-Twist1 transgenic mice are crossed with a Cre recombinase-expressing strain, the floxed (cat) chloramphenicol acetyltransferase gene is deleted and the CAG promoter drives expression of the mouse Twist1, twist homolog 1 (Drosophila), gene in the cre-expressing cells. The floxed (cat) chloramphenicol acetyltransferase gene serves as a reporter/silencer, preventing expression of Twist1 in the absence of Cre recombinase activity, and as a reporter for transgenic expression. Mice homozygous for the transgenic insert are viable, fertile and exhibit no overt phenotype. The Donating Investigator reports that there are no phenotypic differences between hemizygotes a .....
For more information please see the full phenotype on the strain data sheet
012663 FVB.129(B6)-Igf1tm1Dlr/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in the cre-expressing tissue(s).

For example, when crossed to a strain expressing Cre recombinase in early mouse embryos (see Stock No. 003724), this mutant mouse strain may be useful in studies of Igf and postnatal development.

006138 FVB.129(B6)-Smn1tm1Jme/J
Cryopreserved - Ready for recovery
Mice homozygous for this SMNF7 floxed allele are viable and fertile and do not display any gross physical or behavioral abnormalities. Mutant mice exhibit no transcript splicing defects. Cre-mediated recombination of the loxP-flanked sequences results in deletion of exon 7 of the targeted gene. As mutations of this exon are implicated in 95% of all human spinal muscular atrophy (SMA), these mice may be useful in studying SMA or other neuromuscular degenerative diseases.

When crossed to a strain expressing Cre recombinase in neurons (ssee Stock No. 005938, Stock No. 006297, and Stock No. 006663), this mutant mouse strain may be useful as a model of SMA.

When crossed to a strain expressing Cre recombinase in striated muscle fibers (see Stock No. For more information please see the full phenotype on the strain data sheet

005710 FVB.129S-Mmp13tm1Werb/J
Cryopreserved - Ready for recovery
Mice that are homozygous for this loxP-flanked ("floxed") allele are viable and fertile with normal endogenous gene function. Cre-mediated recombination results in replacement of exons 3-5 of targeted gene with a single loxP site. When bred to cre-expressing transgenic strains, these floxed mutant mice may be used to generate whole mouse or tissue-specific targeted mutants that may be useful in examining extracellular matrix remodeling and bone development.

Of note: when these floxed mice are bred to mice containing a beta-actin promoter-driven cre transgene the resulting cre-positive, homozygous-null mice show robust accumulation of cartilage matrix caused by a transient expansion of the hypertrophic zone and increased trabecular bone mass that persists for months.

017441 FVB.129S-Ppp2r1atm1.1Wltr/J
Cryopreserved - Ready for recovery
These F5-6 mutant mice possess loxP sites flanking exons 5-6 of the A-alpha subunit gene (Ppp2r1a) of protein phosphatase 2A (PP2A). Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. PP2A holoenzyme is composed of a catalytic C subunit, a scaffolding A subunit, and a regulatory B subunit. It plays a role in many fundamental cellular processes, such as signal transduction, DNA repair, transcription, translation, growth control, and tumor suppression. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 5-6 deleted in the cre-expressing tissues. This strain can be used for studying all events in which PP2A is involved, including tumor suppression, development, and aging.
008200 FVB/N-Tg(CAG-EGFP,-ALPP)2.6Ggc/J
Cryopreserved - Ready for recovery
Mice harboring the piGAP transgene are viable and fertile, with expression of the eGFP-F-IRES-hPLAP dicistronic gene blocked by an upstream loxP-flanked STOP-polyA sequence. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP-polyA sequence deleted in the cre-expressing tissue(s), permitting dicistronic expression of eGFP-F-IRES-hPLAP. When transgene expression is induced in neurons, human Placental Alkaline Phosphatase (PLAP or ALPP) outlines axonal and dendritic projections and can be visualized by a simple histochemical reaction in fixed cells. Likewise, in vivo fluorescence of farnesylated Enhanced Green Fluorescent Protein (eGFP-F; optimized to target expression to the cytoplasmic side of the plasma membrane) labels axons, and dendrites throughout their length. Because both proteins localize alongside the neuronal surface, concomitant detection of cell body, neurites, and presynaptic and postsynaptic sites may be o .....
For more information please see the full phenotype on the strain data sheet
013063 NOD.129S6(B6N)-S1pr1tm2Rlp/JbsJ
Cryopreserved - Ready for recovery
These S1pr1loxP/loxP mice possess loxP sites flanking exon 2 of the sphingosine-1-phosphate receptor 1 (S1pr1) gene. A floxed-neo cassette is still present downstream of exon 2. S1PR1 is a G protein-coupled receptor for lysophospholipid sphingosine-1-phosphate (S1P) and is highly expressed in endothelial cells. S1PR1 is essential for vascular maturation during embryonic development and is also involved in cell survival, migration, adhesion, and proliferation. This receptor plays a role in the regulation of innate and adaptive immune responses by controlling lymphocyte egress from the thymus, spleen, bone marrow, and lymph nodes. It has also been implicated in tumor angiogenesis and metastasis. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in cre-expressing tissues.

For example, when crossed to B6.Cg-Tg(Tek .....
For more information please see the full phenotype on the strain data sheet

016603 NOD.B6-Gt(ROSA)26Sortm1(HBEGF)Awai/DvsJ
Cryopreserved - Ready for recovery
Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.
For instance, the role of a particular cell population in T1D development can be determined by injecting Diphtheria toxin to deplete the specific cells in a timely fashion.

For example, when bred to a strain expressing Cre recombinase in dendritic cells (see Stock No. 013233 for example), this mutant mouse strain may be useful for studies involving the immunology of diabetes.

When crossed t .....
For more information please see the full phenotype on the strain data sheet

012622 NOD.Cg-Myd88tm1Defr/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s).

These myeloid differentiation primary response gene 88 (Myd88) conditional (floxed) mutant mice may be useful in studies of Toll-like receptor signaling and natural killer cells.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

008008 SJL.129-Cd44tm1Ugu/J
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice possess loxP sites on either side of exon 6 of the targeted gene. RT-PCR analysis reveals that exon 7 is not expressed in the floxed mice, and Southern blots confirm that 1.6kb of exon 7 was deleted during homologous recombination. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 6 and exon 7 deleted in the cre-expressing tissue(s). According to the donating investigator, homozygotes exhibit reduced incidence and severity of induced experimental autoimmune encephalomyelitis (EAE) when compared to wildtype controls. This mutant mouse strain may be useful in studies of multiple sclerosis (MS) and inflammatory diseases.

NOTE: Stock No. 008008 mice are on a unique "SJL/R" genetic background; the "SJL/R" strain was maintained by the Erasmus Medica .....
For more information please see the full phenotype on the strain data sheet

012458 STOCK Adrbk1tm1Gwd/J
Cryopreserved - Ready for recovery
These GRK2f/f mutant mice possess loxP sites flanking exons 3-6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre in a ubiquitous manner embryos die at E~13.5. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 3-6 deleted in the cre-expressing tissues. This strain may be useful for studying the regulation of beta-adrenergic receptor signaling or other receptors regulated in a GRK2-dependent manner.
019001 STOCK Ago1tm1.1Tara/J
Cryopreserved - Ready for recovery
The Argonaute 1 protein, encoded by Eif2c1 (eukaryotic translation initiation factor 2C, 1) is essention to RNA-mediated gene silencing: it binds to both miRNAs and siRNAs, inhibiting mRNA translation. These mice possess loxP sites flanking exons 13 through 16 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these knockout mice are bred to mice that express Cre recombinase, resulting offspring will have exons 13 through 16 deleted in the cre-expressing tissues.
013167 STOCK Ahi1tm2.1Jgg/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 6 and 7 of the Ahi1 (Abelson helper integration site 1) targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of the gene. This strain may be useful in studies of retinopathy, nephronophthisis and Joubert syndrome.
008882 STOCK Bcl2tm1Irt/J
Cryopreserved - Ready for recovery
Mice homozygous for this Bcl2flox conditional allele are viable and fertile, with a loxP-flanked neo cassette upstream of exon 2, as well as a loxP site downstream of exon 2 of the Bcl2 (B-cell leukemia/lymphoma 2) gene. When bred to mice that express Cre recombinase, the resulting offspring can have one of three resulting genotypes in the cre-expressing tissue(s); only the neo selection cassette deleted, only exon 2 deleted, or both the neo selection cassette and exon 2 deleted. The two latter genotypes result in loss of Bcl2 protein expression and are reported to confer the null phenotype. These Bcl2flox mutant mice may be useful in generating conditional mutations for studying apoptosis, mitochondrial permeability, cell survival signaling, cancer, neurological disorders, and immunity.

For example, when crossed to a strain expressing Cre recombinase in myeloid cell lineages (see Stock No. For more information please see the full phenotype on the strain data sheet

008670 STOCK Blmtm4Ches/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 8 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in mammary cells, or a strain with widespread Cre recombinase expression, this mutant mouse strain may be useful in studies of Bloom Syndrome.

017835 STOCK Brca1tm1Aash/J
Cryopreserved - Ready for recovery
The Brca1F22-24/F22-24 allele has loxP sites flanking exons 22-24 of the breast cancer 1 gene (Brca1). Homozygous Brca1F22-24/F22-24 mice are viable and fertile with no observed abnormalities. When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the second terminal BRCT domain deleted in the cre-expressing tissue(s). These Brca1F22-24/F22-24 mice may be useful in generating tissue-specific BRCA1 deletions for studying human basal-like cancer and breast cancer.

For example, when crossed to a strain expressing Cre recombinase in mammary gland during lactation (see Stock No. 017836), mice exhibit increased mammary gland tumor incidence.

018157 STOCK Cdcp1tm1.1Moas/J
Cryopreserved - Ready for recovery
CUB domain-containing protein 1, Cdcp1, is a substrate of Src family kinases and is involved in anoikis resistance and cell adhesion, as well as cancer progression and tumor metastasis. These mice possess loxP sites on either side of exon 1 of the Cdcp1 gene. Exon 1 contains a short 5' UTR, the translational ATG initiation codon, and membrane localization N-terminal signal peptide sequence. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissues.
013701 STOCK Cep290tm1Jgg/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 36 and 37 of the Cep290 (centrosomal protein 290) gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in studies of retinal degeneration, cilia/flagella development, and fertility.
018851 STOCK Dchs1tm1.1Irv/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 2 of the Dchs1 gene. The flanked region involves the first six cadherin domains as well as the initiator methionine and signal peptide.When these mice are bred to mice that express Cre recombinase, the resulting offspring will have the flanked sequence deleted in cre-expressing tissues.

Widespread knockout of Dchs1 (e.g. through crosses with the Tg(Sox2-cre)1Amc strain (Stock No. 004783)) causes defects in multiple internal organs, including kidney, heart, lung and skeleton. Cochleas of homozygous knockouts are shorter than those of wildtype mice. The kidneys are small and cystic. At birth, the lungs are smaller and the intestinal length is reduced. The skeleton exhibits abnormal ossification patterns in the sternum. Although born at predicted Mendelian ratios, homozygous mutants exhibit a variable neonatal lethality, sometimes surviving for two .....
For more information please see the full phenotype on the strain data sheet

013170 STOCK Dclk1tm1.2Jgg/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 3 of the Dclk1 (doublecortin-like kinase 1) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing mouse, this strain is useful in eliminating tissue-specific expression of DCX-domain containing isoforms of this gene. This strain may be useful in studies of neuronal migration.
013172 STOCK Dclk2tm1Jgg/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 2 of the Dclk2 (doublecortin-like kinase 2) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in studies of neurodevelopment and epilepsy.
006001 STOCK Dicer1tm1Bdh/J
Cryopreserved - Ready for recovery
These mice contain loxP sites on either side of exon 23 of the targeted gene. Mice homozygous for this "Dicer-flox" allele are viable and fertile and exhibit no gross phenotypic or behavioral abnormalities. Expression of the targeted allele is indistinguishable from wildtype despite the presence of an frt-flanked neomycin cassette between exon 23 and the 3' loxP site. Cre-mediated recombination (resulting in deletion of exon 23) in the germline leads to developmental arrest at embryonic day 7.5 (E7.5). Tissue specific deletion results in the loss of microRNA (miRNA) processing. Mutant mice can be used to generate cell/tissue-specific deletions of the endogenous gene for applications in embryonic development, translation, protein processing and miRNA/siRNA regulation of gene expression.

For example, when crossed to a strain expressing Cre recombinase in mesenchyme (see Stock No. 005584), this mutant mouse s .....
For more information please see the full phenotype on the strain data sheet

005134 STOCK Disp1tm2Amc/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele, which is hypomorphic.
013073 STOCK Dnm1tm2.1Pdc/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 2-4 of the Dnm1 (dynamin 1) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. A widespread block of expression results in a failure to thrive, defects in neurotransmission and lethality by 2 weeks of age. This strain may be useful in studies of endocytosis.
009063 STOCK Ednrbtm1Nrd/J
Cryopreserved - Ready for recovery
Mice heterozygous for this Ednrbflex3 allele are viable and fertile, with a loxP-flanked neo cassette upstream of exon 3, as well as a loxP site downstream of exon 3 of the Ednrb (endothelin receptor type B or ET-B receptor) gene. When bred to mice that express Cre recombinase, the resulting offspring can have one of three resulting genotypes in the cre-expressing tissue(s); only the neo selection cassette deleted, only exon 3 deleted, or both the neo selection cassette and exon 3 deleted. The two latter genotypes are expected to result in a frameshifted transcript that is reported to confer the null phenotype. These mutant mice may be useful in generating conditional mutations for studying the role of Ednrb in development of melanocytes, development of neurons and glia of the enteric nervous system, neural crest-derived cells, mesenchymal-derived smooth muscle cells, vasodilation, mitogen signaling and cancer, and human Hirschsprung's dis .....
For more information please see the full phenotype on the strain data sheet
018473 STOCK Egfrtm1.1Tyj/J
Cryopreserved - Ready for recovery
Egfr (epidermal growth factor receptor) is a transmembrane protein in the erbB family of tyrosine kinase receptors. Extracellular ligands such as epidermal growth factor (EGF) and transforming growth factor-α (TGF-α) bind EGFR and induce receptor dimerization, autophosphorylation and activation of signal transduction pathways. Mutations in the kinase domain of EGFR, among them L858R (leucine to arginine), are associated with approximately 10% of non-small cell lung cancers (NSCLCs) in the United States and with approximately 35% of cases in Asia.

In this mutant mouse strain, a loxP-flanked Stop cassette(LSL) located in intron 1 of Egfr blocks expression of the gene. Homozygous floxed mice express no EGFR protein are not viable, mimicking the phenotype of the Egfr knockout mice. Upon Cre-mediated excision of the floxed stop cassette, the L858R mutant form of the protein is expressed, albeit at levels lower than those found in wildtype mice. .....
For more information please see the full phenotype on the strain data sheet

009672 STOCK Egln1tm1Kael/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene (widespread deletion of expression is embryonic lethal). This strain may be useful in studies of development and cardiovascular disease.

For example, when crossed to a strain expressing tamoxifen-inducible Cre recombinase in most tissues (see Stock No. 004682), this mutant mouse strain may be useful in studies of polycythemia and congestive heart failure.

When bred to a strain expressing Cre recombinase in cardiac muscle cells (Tg(Myh6-cre)2182Mds, see Stock No. 011038 for example), this mutan .....
For more information please see the full phenotype on the strain data sheet

007918 STOCK En1tm8.1Alj/J
Cryopreserved - Ready for recovery
Mice homozygous for the En1flox conditional allele are viable and fertile, with loxP sites flanking the coding region of exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (encoding the engrailed homeodomain) deleted in the cre-expressing tissue(s). These En1flox mice may be useful in generating conditional mutations for studying engrailed protein function in the embryonic mesencephalon and rhombomere 1, as well as developing cerebellum, limbs, somites, and skin.

Of note, these mice may also be useful in conjunction with other engrailed mutants (such as Stock No. 007912, Stock No. 007916, Stock No. 007917, Stock No. 007924, and Stock No. For more information please see the full phenotype on the strain data sheet

015830 STOCK Erc1tm2.1Sud/J
Cryopreserved - Ready for recovery
A 5'UTR exon and the first coding exon of the Erc1 (ELKS/RAB6-interacting/CAST family member 1; ELKS1, CAST2) gene are flanked by loxP sites in these floxed mutant mice. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Normal expression of the targeted gene is demonstrated by the floxed allele.
015831 STOCK Erc2tm1.2Sud/J
Cryopreserved - Ready for recovery
A 5'UTR exon and the first coding exon of the Erc2 (ELKS/RAB6-interacting/CAST family member 2; ELKS2, CAST1) gene are flanked by loxP sites in these targeted mutant mice. An in-frame tetracysteine tag is located in exon 1. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Normal expression of the targeted gene is demonstrated by the floxed allele.

Widespread cre excision of the floxed exons blocks expression (see Stock No. 008391).

007674 STOCK Esrrbtm1.1Nat/J
Cryopreserved - Ready for recovery
Mice homozygous for this Nr3b2CKO allele possess loxP sites flanking exon 2 of the targeted gene and are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the exon containing the initiator methionine codon and encoding the N-terminal 132 amino acids (including part of the DNA-binding domain) deleted in the cre-expressing tissue(s). Of note, if the conditional Nr3b2CKO is deleted by Cre recombinase in the placenta and embryo, embryonic lethality will result (placental defect). If the conditional Nr3b2CKO is deleted by Cre recombinase only in the embryo, the resulting mice exhibit an inner ear defect (decreased endolymph production) resulting in deafness and defective balance. These Nr3b2CKO mutant mice may be useful in generating conditional mutations to study disorders of hearing and balance, inner ear development (such as endolymph-producing epithelia withi .....
For more information please see the full phenotype on the strain data sheet
007569 STOCK Fgfr2tm1Dor/J
Cryopreserved - Ready for recovery
Mice homozygous for this Fgfr2flox allele possess loxP sites flanking exons 8-10 of the targeted gene and are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have sequences encoding the alternatively spliced Ig domain IIIb, as well as the IIIc and TM domains, deleted in the cre-expressing tissue(s). These Fgfr2-flox mutant mice may be useful in generating conditional mutations to study the role of fibroblast growth factor receptors in vertebrate development; including early embryogenesis, regional specification of the brain, limb morphogenesis, and normal bone, craniofacial, and lens development.

For example, when crossed to a strain expressing Cre recombinase in the central nervous system, especially astrocytes (see Stock No. 004600), this mutant mouse strain may be useful in studies of astroglial migration.

When crossed to .....
For more information please see the full phenotype on the strain data sheet

010907 STOCK Flnatm1.1Caw/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 2-7 of the Flna (filamin, alpha) gene. Mice that are homozygous/hemizygous for this X-linked allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice expressing cre recombinase, the floxed exons are deleted in the tissues expressing cre in the offspring.

When bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 009107 for example), this mutant mouse strain may be useful in studies of vascular development.

008194 STOCK Gata4tm1.1Sad/J
Cryopreserved - Ready for recovery
Mice homozygous for this Gata4loxP conditional allele are viable and fertile, with loxP sites flanking exons 3-5 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for both zinc finger DNA-binding domains and the nuclear localization signal essential for GATA4 function) deleted in the cre-expressing tissue(s). These GATA binding protein 4 conditional mice may be useful in generating conditional mutations for studying GATA4 function during organogenesis (such as cardiogenesis) or in adult mice.

For example, when crossed to a strain expressing Cre recombinase in cardiac myocytes (see Stock No. 009074), this mutant mouse strain may be useful in studies of cardiac hypertrophy, stress-compensation and myocyte viability.

When bred to a strain expressing Cre recombinase in heart muscle (see Stock No. For more information please see the full phenotype on the strain data sheet

008196 STOCK Gata6tm2.1Sad/J
Cryopreserved - Ready for recovery
Mice homozygous for this Gata6loxp conditional allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for the majority of the GATA6 protein) deleted in the cre-expressing tissue(s). These Gata6loxp mice may be useful in generating conditional GATA binding protein 6 mutations for studying GATA6 function in organogenesis or in adult mice.

For example, when bred to a strain expressing Cre recombinase in the villi and crypt cells of the intestine (see Stock No. 004586 for example), or when bred to a strain expressing Cre recombinase in the embryo (see Stock No. 003724, 003314 for example), this mutant mouse strain may be useful in studies of the transcrip .....
For more information please see the full phenotype on the strain data sheet

007927 STOCK Gbx2tm1.1Alj/J
Cryopreserved - Ready for recovery
Mice homozygous for the Gbx2flox conditional allele are viable and fertile, with loxP sites flanking the protein coding region of exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (encoding the gastrulation brain homeobox 2 homeodomain) deleted in the cre-expressing tissue(s). These Gbx2flox mice may be useful in generating conditional mutations for studying gastrulation brain homeobox protein function in the embryonic mesencephalon and rhombomere 1, as well as developing cerebellum and thallamus.
007926 STOCK Gli2tm6Alj/J
Cryopreserved - Ready for recovery
Mice homozygous for this Gli2flox conditional allele are viable and fertile, with loxP sites flanking exons 7 and 8 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have these exons deleted in the cre-expressing tissue(s). This results in a frameshift mutation following splicing of mRNA from exon 6 to 9 and is reported to confer the null phenotype. These Gli2flox mutant mice may be useful in generating conditional mutations for studying Hedgehog/Sonic Hedgehog signaling in the development of many organs (such as central nervous system and axis patterning), as well as the role of Gli2 in adult organs.
008873 STOCK Gli3tm1Alj/J
Cryopreserved - Ready for recovery
Mice homozygous for this Gli3flox conditional allele are viable and fertile, with loxP sites flanking exon 8 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have exon 8 deleted in the cre-expressing tissue(s). This results in a frameshift mutation upstream of the DNA-binding domain following splicing of mRNA from exon 7 to 9 and is reported to confer the null phenotype. These Gli3flox mutant mice may be useful in generating conditional mutations for studying Hedgehog/Sonic Hedgehog signaling in the development of many organs (such as central nervous system and limb patterning), as well as the role of Gli3 in adult organs.

When bred to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771 for example), this mutant mouse strain may be useful in studies of mid/hind brain development.

When bred to a str .....
For more information please see the full phenotype on the strain data sheet

010675 STOCK Gt(ROSA)26Sortm1(CAG-EGFP)Fsh/Mmjax
Cryopreserved - Ready for recovery
Mice homozygous for the R26R CAG-boosted EGFP (RCE) reporter allele harboring both a loxP-flanked STOP cassette and FRT-flanked STOP cassette (RCE:dual) are viable and fertile. Under control of the endogenous Gt(ROSA)26Sor promoter/enhancer regions and the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin (CAG) hybrid promoter, widespread expression of enhanced green fluorescent protein (EGFP) is prevented by the two upstream STOP cassettes. After removal of the flanked STOP cassettes via cre- and Flp-mediated recombination, the EGFP reporter is expressed in cells/tissues where the expression patterns of the individual promoters driving Cre recombinase and FLP recombinase overlap.
005572 STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
Cryopreserved - Ready for recovery
Homozygous mutant mice are viable, fertile, normal in size and do not display any behavioral abnormalities. When these gene targeted mice are bred to transgenic strains expressing Cre recombinase, functional rtTA and EGFP activity is observed in the double mutant offspring in the tissues that express cre. These double mutant mice may be bred to transgenic strains carrying genes of interest under the regulation of tetracycline responsive elements (TRE; tetO) to generate triple mutant mice in which the tissue specificity of the Cre-transgenic line and the doxycycline inducibility of the rtTA/TRE-controlled transgenes can be combined to regulate expression of the target gene.

Of note, mutant mice are also available on a C57BL/6J genetic background (see Stock No. 005670).

008600 STOCK Gt(ROSA)26Sortm1(tTA)Roos/J
Cryopreserved - Ready for recovery
Mice heterozygous for the ROSA:LNL:tTA conditional mutation of the Gt(ROSA)26Sor locus are viable and fertile, with a loxP-flanked STOP cassette preventing transcription of a downstream optimized/modified tetracycline-controlled transactivator protein ("mtTA"). Applying both Cre-lox and Tet-Off technologies, these ROSA:LNL:tTA mutant mice may be useful to generate compound mutant mice in which expression of a tetracycline-responsive promoter element (TRE; also called tet-operator or tetO)-driven gene of interest can be both directed to the cell types defined by the chosen Cre recombinase expression, as well as turned off by the addition of tetracycline (or its analog doxycycline (dox)).
010701 STOCK Gt(ROSA)26Sortm1.1(CAG-EGFP)Fsh/Mmjax
Cryopreserved - Ready for recovery
Mice homozygous for the R26R CAG-boosted EGFP (RCE) reporter allele harboring a loxP-flanked STOP cassette (RCE:loxP) are viable and fertile. Under control of the endogenous Gt(ROSA)26Sor promoter/enhancer regions and the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin (CAG) hybrid promoter, widespread expression of enhanced green fluorescent protein (EGFP) is prevented by the upstream floxed-STOP cassette. After removal of the loxP-flanked STOP cassette by cre-mediated recombination, EGFP reporter expression is directed to the cells/tissues dependent upon expression of the promoter expression driving Cre recombinase.
017922 STOCK Gt(ROSA)26Sortm10(ACTB-tdTomato)Luo/J
Cryopreserved - Ready for recovery
Mice homozygous for the R26TT allele are viable and fertile with no gross behavioral or observable abnormalities. The R26TT allele has a CMV enhancer/chicken beta-actin core promoter-driven "MADM TT" cassette inserted into the Gt(ROSA)26Sor locus on chromosome 6. The "MADM TT" cassette has a complete tdTomato sequence (tagged with three copies of the Myc epitope at its C-terminus). As such, heterozygous and homozygous mice exhibit widespread expression of tdTomato-3Myc (direct fluorescent visualization) throughout the whole animal and all tissues. These R26TT mice are the tdTomato-expressing control for the "new MADM-6" (new mosaic analysis with double markers on chromosome 6) strains, including R26GT (Stock No. 017912) and R26TG (Stock No. 017921). These R26TT mice do not need to be bred with any m .....
For more information please see the full phenotype on the strain data sheet
013124 STOCK Gt(ROSA)26Sortm3(Gli3)Amc/J
Cryopreserved - Ready for recovery
These RosaGli3TFlag c/c mice contain a floxed-neomycin resistance (neo) cassette and polyadenylation signal, cDNA encoding a FLAG-tagged GLI-Kruppel family member (Gli3) repressor gene, an internal ribosome entry site (IRES), and a Venus yellow fluorescent protein (YFP) under control of the ubiquitous Gt(ROSA)26Sor locus. Breeding these mutant mice to mice that express Cre-recombinase will also result in Floxed-neo-stop excision. When these mice are crossed to mice containing Cre-recombinase under direction of a paired related homeobox 1 (Prrx1) promoter (see Stock No. 005584), active in early limb mesenchyme, the mice produce Gli3TFlag at levels that are comparable with the endogenous protein. Mice exhibited a variety of limb defects including a variable preaxial forelimb polydactyly, limb truncation, and reduced mineralization. These mice may be useful for understanding Sonic hedgehog signaling and iden .....
For more information please see the full phenotype on the strain data sheet
009674 STOCK Gt(ROSA)26Sortm4(HIF2A*)Kael/J
Cryopreserved - Ready for recovery
These LSL-HIF2dPA mice conditionally express a form of hemagglutinin (HA)-tagged human HIF2a (HIF2&alpha, HIF2dPA) cDNA that escapes recognition by the von Hippel-Lindau tumor suppressor protein by virtue of proline to alanine substitutions. When crossed with a tissue-specific cre strain, excision of a floxed stop cassette enables expression of the cDNA driven by the endogenous mouse Gt(ROSA)26Sor promoter. There is no expression until the mice are exposed to Cre recombinase. This strain may be useful in studies of von Hippel-Lindau disease mechanisms.

For example, when crossed to a strain expressing Cre recombinase in liver (see Stock No. 003574), this mutant mouse strain may be useful in studies of VHL disease.

When bred to a strain expressing Cre recombinase in cardiac muscle cells (Tg(Myh6-cre)2182Mds, see Stock No. 011038 for example), thi .....
For more information please see the full phenotype on the strain data sheet

012266 STOCK Gt(ROSA)26Sortm5(ACTB-tTA)Luo/J
Cryopreserved - Ready for recovery
Mice homozygous for this ROSA26-ZtTA (or ZtTA) conditional allele are viable and fertile, although the donating investigator reports that heterozygous mice are more healthy and fertile than homozygous mice. This ROSA26-ZtTA (or ZtTA) conditional allele is designed with a loxP-flanked β-geo transcriptional STOP cassette preventing transcription of the downstream tetracycline-controlled transactivator protein (tTA). When bred to mice that express Cre recombinase, offspring will have the STOP cassette deleted and subsequent expression of the tTA in the cre-expressing cells. The donating investigator reports that the CMV enhancer/chicken beta-actin core promoter (pCA) allows stronger and persistent expression of the downstream tTA (especially in adult cells) compared to the endogenous Gt(ROSA)26Sor locus alone. Applying both Cre-lox and Tet-Off technologies, these ZtTA mutant mice may be useful to generate compound mutant mice in which expression of a tetracyclin .....
For more information please see the full phenotype on the strain data sheet
017912 STOCK Gt(ROSA)26Sortm6(ACTB-EGFP*,-tdTomato)Luo/J
Cryopreserved - Ready for recovery
The R26GT allele has a CMV enhancer/chicken beta-actin core promoter-driven "MADM GT" cassette inserted into the Gt(ROSA)26Sor locus on chromosome 6. The "MADM GT" cassette has the N-terminal portion of mut4EGFP, a beta-globin intronic sequence (containing one frt and several lox sites), and a MYC-tagged tdTomato gene lacking an ATG start site (tdT3MycATG-less). Mice homozygous for the R26GT allele are viable with no gross behavioral or observable abnormalities. The donating investigator reports homozygous females have no observed fertility problems, but homozygous males can have reduced fertility. Homozygous mice exhibit no fluorescent protein expression in absence of its reciprocal mutation (even if Cre or FLP recombinase is present).

These mutant mice are designed for "new MADM-6" (new mosaic analysis with double markers on chromosome 6), and must be crossed to mice harboring a reciprocal mutation at the same locus (R26> .....
For more information please see the full phenotype on the strain data sheet

013123 STOCK Gt(ROSA)26Sortm6(Gli1)Amc/J
Cryopreserved - Ready for recovery
These RosaGli1Flag c/c mice contain a floxed-neomycin resistance (neo) cassette and polyadenylation signal, cDNA encoding a FLAG-tagged GLI-Kruppel family member (Gli1) gene, an internal ribosome entry site (IRES), and a Venus yellow fluorescent protein (YFP) under control of the ubiquitous Gt(ROSA)26Sor locus. Breeding these mutant mice to mice that express Cre-recombinase will also result in Floxed-neo-stop excision. When these mice are crossed to mice containing Cre-recombinase under direction of an atonal homolog 1 (Math1) promoter, active in dividing granule neuron precursor cells and medulloblastoma tumors, the mice produce Gli1Flag at levels higher than the endogenous protein in the cerebellum. These mice may be useful for understanding Sonic hedgehog signaling and identifying targets of Gli1 action in developing ventral neural tube.
017921 STOCK Gt(ROSA)26Sortm7(ACTB-EGFP*)Luo/J
Cryopreserved - Ready for recovery
The R26TG allele has a CMV enhancer/chicken beta-actin core promoter-driven "MADM TG" cassette inserted into the Gt(ROSA)26Sor locus on chromosome 6. The "MADM TG" cassette has an ATG start codon, a beta-globin intronic sequence (containing one frt and several lox sites), and a C-terminal portion of mut4EGFP. Mice homozygous for the R26TG allele are viable and fertile, with no gross behavioral or observable abnormalities. Homozygous mice exhibit no fluorescent protein expression in absence of its reciprocal mutation (even if Cre or FLP recombinase is present).

The new TG cassette in R26TG mice is compatible with any "GX cassette" (GFPN-terminus-intron-XATG-less) where XATG-less is any gene without the start codon.

To combine MADM with the Tet-Off binary expression system, R26TG mice must be crossed to mice harboring the R26G-tTA2 allele (Stock No. For more information please see the full phenotype on the strain data sheet

017909 STOCK Gt(ROSA)26Sortm8(ACTB-EGFP*,-tTA2)Luo/J
Cryopreserved - Ready for recovery
The R26G-tTA2 allele has a CMV enhancer/chicken beta-actin core promoter-driven MADM G-tTA2 cassette inserted into the Gt(ROSA)26Sor locus on chromosome 6. The "MADM G-tTA2" cassette has the N-terminal portion of mut4EGFP, a beta-globin intronic sequence (containing one frt and several lox sites), and a tetracycline-regulated transactivator sequence lacking an ATG start site (tTA2ATG-less). Mice homozygous for the R26G-tTA2 allele are viable and fertile, with no gross behavioral or observable abnormalities. Homozygous mice exhibit no fluorescent protein or tTA2 expression in absence of its reciprocal mutation (even if Cre or FLP recombinase is present).

These mutant mice are designed to combine the "new MADM-6" system with a Tet-Off binary expression system to create MADM-Tet (mosaic analysis with double markers combined with Tet-Off), and must be crossed to mice harboring a reciprocal mutation at the same locus (R26TG> .....
For more information please see the full phenotype on the strain data sheet

014178 STOCK Hey2tm1Eno/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene, which encode amino acids 29-82. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 and 3 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in vascular smooth muscle (see Stock No. 004746 for example), this mutant mouse strain may be useful in studies of ventricular septal defect cardiac development.

When bred to a strain with Cre recombinase expression in developing heart, pharynx, this mutant mouse strain may be useful in studies of cardiac development.

017360 STOCK Hilpdatm1.1Nat/J
Cryopreserved - Ready for recovery
The single exon of the 2310016C08Rik gene (commonly called Hig2, hypoxia inducible gene 2; GenBank AF141311) is flanked by loxP sites and followed by a membrane-tethered human placental alkaline phosphatase (hPLAP) in this conditional targeted mutation strain. Expression of the targeted gene is expected to be normal in the conditional allele. Following Cre-mediated recombination, the exon is excised enabling transcription of hPLAP under the control of the Hig2 promoter. hPLAP is expressed only when the Hig2 coding sequences are deleted by Cre-dependent excision. When crossed with a germline Cre strain (Sox2-Cre), expression of hPLAP was found to be widely expressed, with highest levels in the vasculature.
017298 STOCK Hmgn5tm1Mbus/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exons 2 through 4 of the targeted gene, which code for the nucleosome binding domain. Female mice that are homozygous for this allele and male mice that are hemizygous for this X linked allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 4 deleted in the cre-expressing tissue(s).
017530 STOCK Igs2tm2(ACTB-tdTomato,-EGFP)Luo Trp53tm1Tyj Nf1tm1Par/J
Cryopreserved - Ready for recovery
Mice that are heterozygous for the Nf1tm1Par Trp53tm1Tyj alleles and homozygous for the Tg(ACTB-tdTomato,-EGFP)11Luo transgene are viable and fertile. The mosaic analysis with double markers (MADM) system (MADAM strains, Stock No. 013751 and Stock No. 013749), plus the Nf1tm1Par Trp53tm1Tyj alleles, coupled with a Cre recombinase expressing strain allows fluorescent labeling and tracking of cancer cells of origin, which are normal cells that acquire the first cancer conducive mutation(s) and initiate tumorigenesis. Cre-mediated inter-chromosomal recombination in the MADAM system generates a small number of cells homozygous for the double reporter and the mutations, mimicking the intermittent loss of heterozygosity of tumor suppressor genes observed in human cancer. The system also labels the mutant dau .....
For more information please see the full phenotype on the strain data sheet
017932 STOCK Iis3tm1.1(ACTB-EGFP*)Luo/J
Cryopreserved - Ready for recovery
The Miya10TG (M10TG) transgene has the CMV enhancer/chicken beta-actin core promoter upstream of a frt-flanked "MADM TG" cassette, all inserted into an intergenic region on chromosome 10 (~21 Mbp; between the Ahi1 and Myb loci). The "MADM TG" cassette has an ATG start codon, a beta-globin intronic sequence, and the C-terminal portion of a mutant enhanced green fluorescent protein (mut4EGFP). Homozygous M10TG mice are viable and fertile with no gross behavioral or observable abnormalities. Homozygous mice exhibit no fluorescent protein expression in absence of its reciprocal mutation (even if Cre recombinase is present).

These mutant mice are designed for MADM-10 (mosaic analysis with double markers on chromosome 10), and must be crossed to mice harboring a reciprocal transgene at the same locus (M10GT mice; Stock No. 017923) to allow Cre recombinase-induced .....
For more information please see the full phenotype on the strain data sheet

017923 STOCK Iis3tm2.1(ACTB-EGFP*,-tdTomato)Luo/J
Cryopreserved - Ready for recovery
The Miya10GT (M10GT) transgene has the CMV enhancer/chicken beta-actin core promoter upstream of a frt-flanked "MADM GT" cassette, all inserted into an intergenic region on chromosome 10 (~21 Mbp; between the Ahi1 and Myb loci). The "MADM GT" cassette has the N-terminal portion of mut4EGFP, a beta-globin intronic sequence, and a MYC-tagged tdTomato gene lacking an ATG start site (tdT3MycATG-less). Homozygous M10GT mice are viable and fertile with no gross behavioral or observable abnormalities. Homozygous mice exhibit no fluorescent protein expression in absence of its reciprocal mutation (even if Cre recombinase is present).

These mutant mice are designed for MADM-10 (mosaic analysis with double markers on chromosome 10), and must be crossed to mice harboring a reciprocal transgene at the same locus (M10TG mice; Stock No. 017932) to allow Cre recomb .....
For more information please see the full phenotype on the strain data sheet

021458 STOCK Iis5tm1(ACTB-tdTomato,-EGFP)Luo/J
Cryopreserved - Ready for recovery
Mice homozygous for the MADM-7TG (Hipp7TG) allele are viable and fertile with no reported abnormalities. The MADM-7TG allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of tdTomato, a beta-globin intronic sequence (containing a frt-flanked region of two lox site variants [lox2272 and lox5171] and then a loxP-flanked neomycin resistance gene), and the C-terminal portion of mut4-EGFP all inserted into the Hipp7 locus on chromosome 7 (~2.13 cM; ~0.7 kbp downstream of exon 5 of the Rps9 gene). These MADM-7TG mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to MADM-7GT mice harboring a reciprocal mutation at the same locus (Stock No. 021457). The resulting TG/GT offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-het .....
For more information please see the full phenotype on the strain data sheet
021457 STOCK Iis5tm2.1(ACTB-EGFP,-tdTomato)Luo/J
Cryopreserved - Ready for recovery
Mice homozygous for the MADM-7GT (Hipp7GT) allele are viable and fertile with no reported abnormalities. The MADM-7GT allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of mut4-EGFP, a beta-globin intronic sequence (containing a frt-flanked region of two lox site variants [lox2272 and lox5171] and then a single loxP site), and the MYC-tagged C-terminal portion of tdTomato all inserted into the Hipp7 locus on chromosome 7 (~2.13 cM; ~0.7 kbp downstream of exon 5 of the Rps9 gene). These MADM-7GT mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to MADM-7TG mice harboring a reciprocal mutation at the same locus (Stock No. 021458). The resulting GT/TG offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous") .....
For more information please see the full phenotype on the strain data sheet
021461 STOCK Iis6tm1.1(ACTB-tdTomato,-EFGP)Luo/J
Cryopreserved - Ready for recovery
Mice homozygous for the MADM-12TG (John12TG) allele are viable and fertile with no reported abnormalities. The MADM-12TG allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of tdTomato, a beta-globin intronic sequence (containing an frt site and a loxP site), and the C-terminal portion of mut4-EGFP all inserted into the John12 locus on chromosome 12 (~1.71 cM; ~16 kbp downstream of exon 1 of the Rab10 gene). These MADM-12TG mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to MADM-12GT mice harboring a reciprocal mutation at the same locus (Stock No. 021460). The resulting TG/GT offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous"), and must also be bred to harbor a Cre- or FLP-recombinase to induce fluorescent protein ex .....
For more information please see the full phenotype on the strain data sheet
021460 STOCK Iis6tm2.1(ACTB-EFGP,-tdTomato)Luo/J
Cryopreserved - Ready for recovery
Mice homozygous for the MADM-12GT (John12GT) allele are viable and fertile with no reported abnormalities. The MADM-12GT allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of mut4-EGFP, a beta-globin intronic sequence (containing an frt site and a loxP site), and the MYC-tagged C-terminal portion of tdTomato all inserted into the John12 locus on chromosome 12 (~1.71 cM; ~16 kbp downstream of exon 1 of the Rab10 gene). These MADM-12GT mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to MADM-12TG mice harboring a reciprocal mutation at the same locus (Stock No. 021461). The resulting GT/TG offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous"), and must also be bred to harbor a Cre- or FLP-recombinase to induce fluorescent pro .....
For more information please see the full phenotype on the strain data sheet
022142 STOCK Il2rgtm1.1Asin/J
Cryopreserved - Ready for recovery
The IL7 receptor is important in the regulation of T, B, and NK lymphoid cell development. The Il2rg, interleukin 2 receptor, gamma chain, gene encodes the cytokine receptor common gamma chain subunit glycoprotein, a component common to the IL7, IL2, IL4, IL9, IL15 and IL21 receptors. These gammacfl mice possess loxP sites flanking exons 2 through 6 of the targeted gene. Male hemizygous and female homozygous (the gene is X linked) for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 6 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in preselection double positive thymocytes, this mutant mouse strain may be useful in studies of T cell population subset differentiation in the thymus.

017575 STOCK Juptm1.1Glr/J
Cryopreserved - Ready for recovery
These PGflox mutant mice possess loxP sites flanking exon 1 of the junction plakoglobin (Jup) gene. PG is a member of the armadillo protein family found in submembranous plaques of desmosomes and adherens junctions where it directly interacts with cadherins. Mutations in PG have been found in cases of Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC). Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissues. For example, when bred to mice carrying the Tg(Myh6-cre/Esr1*)1Jmk transgene (see Stock No. 005657), PG deletion in the adult myocardium after tamoxifen induction results in progressive loss of cardiac myocytes, extensive inflammatory infiltration, fi .....
For more information please see the full phenotype on the strain data sheet
005994 STOCK Mbtps1tm1Jdh/J
Cryopreserved - Ready for recovery
These mice carry a targeted mutation in which exon 2 of the targeted gene is flanked by loxP sites. A loxP-flanked ("floxed") neomycin resistance cassette also is inserted downstream in intron 2. Homozygotes are viable and fertile, and the floxed gene appears to function normally. When homozygotes are crossed with transgenic strains expressing Cre-recombinase, cre-mediated recombination of the loxP-flanked sequences can result in one of three genotypes: a) deletion of the neo cassette only, leaving a loxP-flanked second exon and unimpaired endogenous gene function. b) Deletion of exon 2 only, leaving a loxP-flanked neo cassette and no endogenous gene function. c) Deletion of both the neo cassette and exon 2, leaving a single loxP site and no endogenous gene function. When these floxed mutant mice are bred to mice carrying the Mx1-cre transgene (for example, Stock No. 003556), liver- .....
For more information please see the full phenotype on the strain data sheet
016950 STOCK Mir10btm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked Mir10b stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and Mir10b stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
017506 STOCK Mir124a-3tm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR124a-3 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR124a-3 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
017507 STOCK Mir129-1tm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR129-1 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR129-1 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
016945 STOCK Mir138-1tm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR138-1 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR138-1 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
017509 STOCK Mir138-2tm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR138-2 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR138-2 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
016954 STOCK Mir187tm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR187 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR187 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
017510 STOCK Mir188tm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR188 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR188 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
017511 STOCK Mir194-2tm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR194-2 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR194-2 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
016953 STOCK Mir196a-1tm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked Mir196a-1 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and Mir196a-1 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
017512 STOCK Mir199a-1tm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked mir199a-1 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and mir199a-1 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
016952 STOCK Mir219a-2tm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked Mir219-2 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and Mir219-2 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
016949 STOCK Mir21atm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked Mir21 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and Mir21 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
017505 STOCK Mir23btm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR23b stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR23b stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
016845 STOCK Mir320tm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR320 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR320 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
021476 STOCK Mir325tm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable and fertile. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR325 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR325 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). LacZ expression is observed in the central nervous system, brain, spinal cord and visual sensory system. This mutant mouse strain may be useful in studies of microRNA biology. Additional information about this mirKO mouse can be found here.
016843 STOCK Mir7btm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR-7b stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR-7b stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). LacZ expression is observed in embryonic brain and spinal cord. This mutant mouse strain may be useful in studies of microRNA biology.
016846 STOCK Mir9-1tm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR9-1 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR9-1 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). LacZ expression is observed in embryonic brain and spinal cord. This mutant mouse strain may be useful in studies of microRNA biology.
016951 STOCK Mir9-2tm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked Mir9-2 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and Mir9-2 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
016946 STOCK Mirc29tm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR29b-2/miR29c (Mirc29) stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR29b-2/miR29c stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
017504 STOCK Mirc30tm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miR15a/16-1 (Mirc30) stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miR15a/16-1 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.
017514 STOCK Mirc31tm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miRlet7c-2/7b (Mirc31) stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miRlet7c-2/7b stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). LacZ expression is observed in embryonic brain and spinal cord. This mutant mouse strain may be useful in studies of microRNA biology.
016844 STOCK Mirlet7dtm1Mtm/Mmjax
Cryopreserved - Ready for recovery
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked miRlet7d stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and miRlet7d stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). LacZ expression is observed in embryonic brain and spinal cord. This mutant mouse strain may be useful in studies of microRNA biology.
010928 STOCK Mnx1tm1Spf/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon3 of the Mnx1 (motor neuron and pancreas homeobox 1) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene, exon 3 is deleted in cre-expressing tissues in the offspring. This strain may be useful in studies of neurodevelopment.
014179 STOCK Mov10l1tm1.1Eno/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 20, which encodes a helicase domain. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 20 deleted in the cre-expressing tissue(s).

When bred to a strain with widespread expression of Cre recombinase, this mutant mouse strain may be useful in studies of retrotransposon activiation in male germ cells. Global homozygous deletion results in male infertility due to apoptosis of pachytene spermatocytes.

017632 STOCK Ncor1tm1Anh/J
Cryopreserved - Ready for recovery
These NCoRlox mutant mice possess loxP sites flanking exons 37-40 of the nuclear receptor co-repressor 1 (Ncor1) gene. Exons 37-40 encode two of three 5' receptor interacting domains (RIDs). NCOR1 is recruited through interactions of its RIDs to nuclear receptors, such as retinoic-acid and thyroid-hormone receptors (TR), where it acts as a transcription repressor. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 37-40 deleted in the cre-expressing tissues. For example, when bred to B6.Cg-Tg(Alb-cre)21Mgn/J mice (Stock No. 003574) deletion of the two most 5' RIDs (N2 and N3) in the liver results in the inability of NCOR1 to bind to the TR. This strain may be useful for studying the role of NCOR1 on .....
For more information please see the full phenotype on the strain data sheet
015835 STOCK Nlgn3tm2Sud/J
Cryopreserved - Ready for recovery
This Nlgn3 (neuroligin 3) strain carries a hypomorphic mutation created by replacing the endogenous signal sequence coding region in exon 2 with preprotrypsin signal sequence. Expression of the X-linked targeted gene is reduced to approximately 10% that of wildtype levels in brain tissue.

Exons 2 and 3 are flanked by loxP sites. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. Widespread Cre excision of the floxed exons blocks expression of the gene (see Stock No. 008394).

016117 STOCK Nogtm1.1Rmh/J
Cryopreserved - Ready for recovery
In this strain loxP sites flank the coding region of the Noggin (Nog) gene. Homozygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. NOGGIN is a secreted protein involved in embryonic development including bone development, joint formation, and neural tube fusion. It also plays a role in neural induction, where it inhibits the bone morphogenetic protein (BMP)-signaling pathway. NOGGIN also inhibits Transforming growth factor (TGF)-β signal transduction by binding to TGF-β family ligands and preventing them from binding to their corresponding receptors. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will not express NOGGIN in the cre-expressing tissues. This strain may be useful for studying BMP-signaling during embryonic development and in postnatal tissues.

For example, when crossed to a strain expressing Cre recombinase in embryonic cells (see .....
For more information please see the full phenotype on the strain data sheet

006951 STOCK Notch1tm2Rko/GridJ
Cryopreserved - Ready for recovery
Mice homozygous for this "floxed" Notch1 allele (fN1) are viable and fertile. In the targeted allele, loxP sites were placed flanking exon 1 of the targeted gene. When these floxed mice are bred to mice expressing Cre recombinase, exon 1 of the targeted gene is deleted in cre-expressing tissue(s) in the cre-positive, homozygous floxed offspring. These conditional knockout mice may be useful in generating tissue-specific mutants for studying the development of a wide range of tissues: for example, when crossed to a strain expressing Cre recombinase primarily in the nervous system (see Stock No. 003771), this mutant strain may be useful in studies of apoptosis in neural development.

When crossed to a strain expressing a differential Cre mediated reporter protein labeling: Notch1 signaling in actively cycling stem/progenitor cells (see Stock No. 006953 .....
For more information please see the full phenotype on the strain data sheet

005384 STOCK Numbtm1Zili Numbltm1Zili/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 1 of the Numb gene and loxP sites flanking exons 1 through 3 of the Numbl gene. Mice that are homozygous for these alleles are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.

For example, when crossed to a strain expressing an interferon inducible Cre recombinase (see Stock No. 003556), this mutant mouse strain provides information about hemopoiesis and lymphopoesis.

018432 STOCK Pdgfratm13Sor/J
Cryopreserved - Ready for recovery
The PDGFRα(S)J conditional knockin allele has the endogenous PDGFRα sequences replaced with a loxP-flanked PGKneo (lox-stop-lox) cassette upstream of the constitutively active PDGFRαJ mutant isoform. This V561D mutation in the juxtamembrane domain disrupts inhibitory contacts between the juxtamembrane and kinase domains, which are important for full auto-inhibition, and results in constitutive PDGFRα activity. Prior to Cre recombinase exposure, no expression of the constitutively active αJ mutant isoform is observed, and the mutant allele functions as a knockout. Mice homozygous for the PDGFRα(S)J allele die during embryonic development. Heterozygous PDGFRα(S)J mice are viable and fertile with no reported abnormalities. Following Cre recombinase-mediated excision of the floxed-STOP cassette, high basal kinase activity without the addition of ligand is observed in the cre-expressing tissues. > .....
For more information please see the full phenotype on the strain data sheet
018434 STOCK Pdgfrbtm13(Pdgfrb)Sor/J
Cryopreserved - Ready for recovery
The PDGFRβ(S)J conditional knockin allele has the endogenous PDGFRβ sequences replaced with a loxP-flanked PGKneo (lox-stop-lox) cassette upstream of the constitutively active PDGFRβJ mutant isoform (V536A mutation in the juxtamembrane domain disrupts a highly conserved juxtamembrane region in type III receptor tyrosine kinases that contacts the kinase domain important for full auto-inhibition, leading to constitutive PDGFRβ activity). Prior to Cre recombinase exposure, no expression of the constitutively active βJ mutant isoform is observed, and the mutant allele functions as a knockout. Mice homozygous for the PDGFRβ(S)J allele are neonatal lethal. Heterozygous PDGFRβ(S)J mice are viable and fertile with no reported abnormalities. Following Cre recombinase-mediated excision of the floxed-STOP cassette, high basal kinase activity without the addition of ligand is observed in the cre-expressing tissues. .....
For more information please see the full phenotype on the strain data sheet
018435 STOCK Pdgfrbtm14(Pdgfrb)Sor/J
Cryopreserved - Ready for recovery
The PDGFRβ(S)K conditional knockin allele has the endogenous PDGFRβ sequences replaced with a loxP-flanked PGKneo (lox-stop-lox) cassette upstream of the constitutively active PDGFRβK mutant isoform (D849V mutation in the kinase domain interferes with the inactive conformation of the ATP-binding pocket; leading to constitutive PDGFRβ activity). Prior to Cre recombinase exposure, no expression of the constitutively active βK mutant isoform is observed, and the mutant allele functions as a knockout. Mice homozygous for the PDGFRβ(S)K allele are neonatal lethal. Heterozygous PDGFRβ(S)K mice are viable and fertile with no reported abnormalities. Following Cre recombinase-mediated excision of the floxed-STOP cassette, high basal kinase activity without the addition of ligand is observed in the cre-expressing tissues.

Of note, pan-deletion of the floxed-STOP cassette can be achieved by breeding PDGFR&be .....
For more information please see the full phenotype on the strain data sheet

012871 STOCK Pik3r1tm1Lca/J
Cryopreserved - Ready for recovery
Mice homozygous for this p85αloxP allele are viable and fertile, with loxP sites flanking exon 7 of the targeted gene. The Pik3r1 locus encodes three proteins (p85α, p55α, and p50α) that arise from alternative transcription initiation sites; and exon 7 is the first common exon for all three isoforms. When bred to mice that express Cre recombinase, the resulting offspring will have exon 7 deleted in the cre-expressing tissue(s); splicing of upstream exons (exon 6, 1b, or 1c) directly into the downstream exon 8 results in a frameshift mutation that introduces an immediate stop codon. Such a deletion should prevent the translation of the SH2 and p110-binding domains, eliminating the ability to form a functional protein from any of the three transcription initiation sites. These mutant mice may be useful in generating conditional mutations for studying class IA phosphoinositide 3-kinases (PI3Ks) in cell growth, cell proliferation cell .....
For more information please see the full phenotype on the strain data sheet
017569 STOCK Polr2atm1(cre/ERT2)Bbd E4f1tm1.1Llca/J
Cryopreserved - Ready for recovery
Mice that are homozygous for both of the targeted mutations are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These double mutant mice carry the floxed E4f1tm1.1Llca allele and the CreERT2 knock in allele, Polr2atm1(cre/ERT2)Bbd. Topical applications of tamoxifen on bare skin result in deletion of the floxed sequences in the treated skin, with epidermal hyperplasia, epidermal cell loss and severe skin ulceration 1 to 2 weeks after application. Tamoxifen treated epidermal basal cells do not exhibit mitotic abnormalities.

The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, C .....
For more information please see the full phenotype on the strain data sheet

017880 STOCK Pomt2tm1.1Hhu/J
Cryopreserved - Ready for recovery
These Pomt2f/f mutant mice possess loxP sites flanking exons 2-4 of the protein-O-mannosyltransferase 2 (Pomt2) gene. Mice that are homozygous for this allele are viable and fertile. Pomt2 encodes a glycosyltransferase expressed on the membrane of the endoplasmic reticulum which is required for the initiation of O-mannosyl glycans biosynthesis. Mutations in POMT2 are associated with central nervous system malformations including muscle-eye-brain disease and Walker-Warburg syndrome (WWS). WWS is characterized by lissencephaly, mental retardation, progressive muscle weakness, and structural defects of the cerebellum, brain stem, and eyes. Breeding these mice to mice that express Cre recombinase results in offspring with exons 2-4 deleted in the cre-expressing tissues.

For example, when bred to B6.129S2-Emx1tm1(cre)Krj/J mice (Stock No. 005628), POMT2 .....
For more information please see the full phenotype on the strain data sheet

005737 STOCK Ppiftm1Mmos/J
Cryopreserved - Ready for recovery
In this strain loxP sites flank exons 3-5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cre-mediated recombination results in the deletion of the loxP-flanked region. This strain represents an effective tool for generating tissue-specific targeted mutants useful in studies examining the consequences of disrupting Ppif-dependent pathways.
010499 STOCK Pygo2tm1.1Ssp/J
Cryopreserved - Ready for recovery
Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Excision of the floxed fragment in eye tissues results in microophthalmia and abnormal lens induction. This mutant mouse strain is useful in studies of lens development, but may also be useful in studies of other organs as well.

For example, when crossed to a strain with widespread Cre recombinase expression (see Stock No. 003465 and 006054), this mutant mouse strain may be useful in lens development.

When crossed to a strain expressing Cre recombinase in midbrain and spinal cord (see Stock No. 007807), this mutant mouse strain may b .....
For more information please see the full phenotype on the strain data sheet

015832 STOCK Rims1tm3Sud/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 6 in the Rims1 (regulating synaptic membrane exocytosis 1; Rim1α/β) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.
015833 STOCK Rims2tm1.1Sud/J
Cryopreserved - Ready for recovery
These mice possess loxP sites on either side of exon 26 in the Rims2 (regulating synaptic membrane exocytosis 2) gene. An in-frame ECFP-tetracysteine tag is fused to the floxed exon, enabling immunofluorescent detection. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the α, β, and γ isoforms of the gene.
007570 STOCK Sim1tm1.2Az/J
Cryopreserved - Ready for recovery
Mice homozygous for this "Sim1-floxed exon 1" (2-loxP) conditional allele are viable and fertile, with loxP sites flanking the translation start site and the first 17 amino acids of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for the basic domain of SIM1) deleted in the cre-expressing tissue(s). These "Sim1-floxed exon 1" (2-loxP) mice may be useful in generating conditional mutations for studying basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS) transcription factors, central nervous system development, early-onset/hyperphagic obesity, and regulation of appetite and energy balance.
015836 STOCK Syt12tm1.1Sud/J
Cryopreserved - Ready for recovery
Exon 4 of this Syt12 (synaptotagmin XII) targeted mutation strain is flanked by loxP sites and carries a serine to alanine mutation at residue 97 (S97A). Serine 97 is a protein kinase A (PKA) phosphorylation site and the point mutation abolishes the increase of spontaneous neurotransmitter release by Syt12 overexpression in primary cortical culture. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. Floxed mice express the targeted gene at levels comparable to wild type, as demonstrated by Western blot of whole brain.
016539 STOCK Tdgf1tm2.2Mms/J
Cryopreserved - Ready for recovery
These Cripto2loxP mice possess a loxP-sites flanking exons 3-5 of the teratocarcinoma-derived growth factor 1 (Tdgf1) gene. Homozygous mice are viable, fertile, and normal in size. Cripto, a small extracellular protein containing a divergent epidermal growth factor (EGF) motif and a conserved cysteine-rich (CFC) domain, is essential for formation of the notochordal plate, prechordal mesoderm and foregut endoderm during gastrulation. Expressed in precursors of the axial mesendoderm and definitive endoderm, Cripto is a glycosyl-phosphatidylinositol (GPI)-linked protein that can mediate signaling through Nodal, a factor that is required for axial mesendoderm formation. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3-5 deleted in cre-expressing tissues. This strain may be useful for studying embryonic development and tumor growth.
018784 STOCK Trpm7tm1Clph/J
Cryopreserved - Ready for recovery
TRPM7 is a transient receptor potential (TRP) cation-permeant ion channel that functions as an ion channel and a kinase. TRPM7 is expressed in the early embryo, predominantly in the fetal heart at E9.5, but expanding throughout the embryo by E14.5. These mutant mice possess loxP sites flanking exon 17 of the (Trpm7) gene. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express Cre recombinase, the resulting offspring will have exon 17 deleted in the cre-expressing tissues. These mice may be useful in generating conditional knockouts for studying the role of embryogenesis and thymopoiesis.

For example, when bred to mice carrying Tg(Lck-cre)548 Jxm (see Stock No. 003802), Cre recombinase expression in thymocytes results in a block in thymopoiesis at the double-negative stage, depletion of thymic medullary cells, reduced numbers of T cells and aberrant synthesis of several .....
For more information please see the full phenotype on the strain data sheet

008469 STOCK Wnt9btm1.2Amc/J
Cryopreserved - Ready for recovery
Mice homozygous for the Wnt9bc allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). Such deletion is predicted to result in out-of-frame splicing of exon 1 to exon 3, and consequently a mutant transcript that would encode a nonfunctional peptide comprising the first 27 amino acids of Wnt9b that includes the signal peptide. These Wnt9bc mice may be useful in generating conditional mutations for studying the role of Wnt9b (and other Wnt family members) in development and canonical Wnt signaling cascades, including metanephric kidney and urogenital system development.
014092 STOCK Tg(ACTB-tTA2,-MAPT/lacZ)1Luo/J
Cryopreserved - Ready for recovery
Homozygous CAG-stop-tTA2 transgenic mice are viable and fertile. CAG-stop-tTA2 transgenic mice harbor the ii-CAG-stop-tTA2-IRES-tau-lacZ-ii transgene; designed with a loxP-flanked transcriptional STOP cassette preventing transcription of the downstream modified tetracycline-regulated transactivator (tTA2). The ii-CAG-stop-tTA2-IRES-tau-lacZ-ii transgene is flanked by two copies of the chicken β-globin HS4 insulator on each side to preserve expression fidelity (see additional information below). When bred to mice that express a tamoxifen-inducible Cre recombinase (CreERT2), administration of tamoxifen to the double mutant offspring allows the CreERT2 fusion protein to enter the nucleus of the cre-expressing cells; this deletes the STOP cassette and results in expression of tTA2. The donating investigator reports that tau-lacZ fusion protein expression in the tamoxifen-treated double mutant offspring is faint. Of note, the donating investigator h .....
For more information please see the full phenotype on the strain data sheet
006882 STOCK Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe/J
Cryopreserved - Ready for recovery
Mice hemizygous for this "Z/AP-AML1-ETO" transgene (coding for the translocation t(8;21) present in 15% of acute myeloid leukemias (AML)) are viable and fertile. Homozygotes die in utero presumably due to high lacZ expression. Prior to cre-mediated excision of the "floxed" STOP sequence, expression of lacZ is observed in all tissues including bone marrow progenitor cells. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the human AML1-ETO fusion protein and placental alkaline phosphatase (ALPP or PLAP) to proceed in the Cre recombinase expressing cells. While pan expression of AML1-ETO leads to embryonic lethality (E7.5), hematopoietic and endothelial expression leads to malignancy in B- and T- lymphoid cells and secondary mutations that closely resemble the association of AML1-ETO with acute myeloid leukemia in humans. These transgenic mice may .....
For more information please see the full phenotype on the strain data sheet
005438 STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
Cryopreserved - Ready for recovery
While mice hemizygous for this Z/RED transgene are reported to be viable and fertile, it has been our experience at The Jackson Laboratory that hemizygous animals are often smaller than littermates and subject to postnatal mortality. Delayed weaning greatly enhances the survival. Although homozygous animals are born, animals have not survived past five weeks of age. These transgenic mice express beta-galactosidase under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with red fluorescent protein (DsRed*MST) expression in tissues expressing Cre recombinase. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells.
006850 STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
Cryopreserved - Ready for recovery
Mice hemizygous for this Cre-conditional IC-Notch (or Z/EG-Notch) transgene are viable and fertile. Homozygotes die in utero, presumably due to high lacZ expression. Prior to Cre-mediated excision of the "floxed" STOP sequence, high expression of lacZ is observed in cells and tissues. When bred to Cre recombinase expressing mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the intracellular human NOTCH1 (IC-Notch) and EGFP to proceed in the Cre recombinase expressing cells. For example, endothelial expression of IC-Notch using different Cre-transgenic matings is associated with neural, somite and angiogenic defects, infertility in females, and embryonic lethality in the resulting offspring. These transgenic mice may be useful for global expression of lacZ or, when crossed to a Cre recombinase expressing strain, for studying the role of Notch signaling during both embryonic develo .....
For more information please see the full phenotype on the strain data sheet
006876 STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
Cryopreserved - Ready for recovery
Mice hemizygous for this Cre-conditional TEL-AML1 (or iZ/EG-TEL-AML1) transgene are viable and fertile. Homozygotes die in utero, presumably due to high lacZ expression. Prior to Cre-mediated excision of the "floxed" STOP sequence, high expression of lacZ is observed in cells and tissues. When bred to Cre recombinase transgenic mice, the STOP sequence (and beta-geo) is removed in the resulting offspring, allowing transcription/co-expression of both the human TEL-AML1 fusion protein and EGFP in all cre-expressing cells. TEL-AML1 transcripts are not observed in adult organ tissues prior to excision of the floxed sequences. Following Cre-mediated deletion of the STOP sequence (by B6.Cg-Tg(Tek-cre)12Flv/J, Stock No. 004128), Western blot analysis reveals that EGFP levels are well correlated with TEL-AML1 transcript levels. While global expression of TEL-AML1 leads to embryonic lethality (E7.5), hematopoieti .....
For more information please see the full phenotype on the strain data sheet
003919 STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J
Cryopreserved - Ready for recovery
These transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being erythrocytes, chondrocytes and adipocytes. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with alkaline phosphatase expression in tissues expressing Cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess cre excision activity at the individual cell level.
008783 STOCK Tg(CAG-cre/Esr1*)5Amc Smn1tm3(SMN2/Smn1)Mrph Tg(SMN2*delta7)4299Ahmb Tg(SMN2)89Ahmb/J
Cryopreserved - Ready for recovery
These SMN2;Δ7;Cre-ER;SmnRes mice harbor multiple mutations/transgenes.
The Smn rescue allele (SmnRes; also called Smn1 conditional inversion or Smn1 COIN) is a functional null in the non-recombined state. Prior to Cre recombination, no full-length SMN transcript is detected in somatic tissue by RT-PCR. No spontaneous inversion of the allele is reported in the absence of Cre recombinase. The SmnRes allele is designed to revert to a fully functional SMN upon exposure to Cre recombinase.
Mice homozygous for the Tg(SMN2)89 transgene (SMN2+/+), homozygous for the Tg(SMNΔ7)4299 transgene (SMNΔ7+/+), and homozygous for the Smn1tm3(SMN2/Smn1)Mrph mutation (SmnRes/Res) are a moderate Type II SMA mouse model with similar phenotype as Stock No. 005025. Those SMN2+/+;SMNΔ7+/+;SmnRes/Res mice exhibi .....
For more information please see the full phenotype on the strain data sheet

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New Strains Awaiting Transfer from the Donor

(See informational text following listing of strains)
How to Register Interest
Please indicate your interest in purchasing any of the strains listed below when they become available for distribution by checking the box next to the strain(s) of interest and then selecting the "Continue" button which leads to an Interest Form.

View a Data sheet for New Strains Awaiting Transfer
Select the strain name to link to the strain data sheet.

Stock
Number
Strain Name
 
Strain Description
Standard Supply
021886129-Mirc14tm1.1Czc/J
Awaiting Transfer from the Donor
In this conditional mutation strain, microRNA mir-181ab1 (Mir181a-1-Mir181b-1; also called Mirc14) is flanked by loxP sites. Cre excision of the floxed region enables tissue/cell-specific knockouts of the genes. Germline deletion reveals that Mir181a-1-Mir181b-1 controls the development of normal thymic T cells and inhibits the development of Notch1 oncogene-induced T cell acute lymphoblastic leukemia (T-ALL). These are potential targets for inhibiting tumor development that evoke little toxicity to normal development.
014167129S-Dicer1tm1.1Mnn/J
Awaiting Transfer from the Donor
These Dicer1c mutant mice possess loxP sites flanking exons 25-27 of the Dcr-1 homolog (Dicer1) gene.
021788129S-H3f3atm1.1Mnn/J
Awaiting Transfer from the Donor
These H3f3ac/c mutant mice possess loxP sites flanking a wildtype H3 histone isoform 3 (H3.3) coding sequence and STOP sequence. When bred to mice expressing cre-recombinase, removal of the floxed sequence results in YFP expression. This strain may be useful for studying the role of histone H3.3 during development.
021789129S-H3f3btm1.1Mnn/J
Awaiting Transfer from the Donor
These H3f3bc/c mutant mice possess loxP sites flanking a wildtype H3 histone isoform 3 (H3.3) coding sequence and STOP sequence. When bred to mice expressing cre-recombinase, removal of the floxed sequence results in CFP expression. This strain may be useful for studying the role of histone H3.3 during development.
024877B6.129(Cg)-Fa2htm1.2Hama/J
Awaiting Transfer from the Donor
These Fa2hflox/flox mice may be useful for studying the lipid profile of myelin disorders of the central nervous system.
024414B6.129(SJL)-Grip1tm1Rlh/J
Awaiting Transfer from the Donor
The first PDZ domain of Grip1 is flanked by loxP sites in this conditional knockout strain. This enables the development of Cre-mediated knockouts useful in studies of synaptic plasticity.
025438B6.129-Paip2tm1.1Nso/J
Awaiting Transfer from the Donor
These floxed mutant mice possess loxP sites flanking exons 2 and 3 of the Paip2 (Paip2a) gene. This strain may be useful for generating conditional mutations in applications related to the regulation of mRNA translation and spermiogenesis.
025437B6.129-Paip2btm1.1Nso/J
Awaiting Transfer from the Donor
These floxed mutant mice possess loxP sites flanking exon 3 of the Paip2b gene. This strain may be useful for generating conditional mutations in applications related to the regulation of mRNA translation and spermiogenesis.
024992B6.129-Prdm16tm1.1Brsp/J
Awaiting Transfer from the Donor
Prdm16lox/lox floxed mice may be useful for studying the role PRDM16 plays in the regulation of thermogenesis, obesity, and diabetes.
024417B6.129S6(SJL)-Prkcztm1.1Rlh/J
Awaiting Transfer from the Donor
The catalytic domain of the mouse Prkcz gene is flanked by loxP sites in this conditional mutant strain. It enables the creation of Cre-mediated PKM-ζ isoform knockouts, useful in studies of synaptic plasticity, learning and memory.
024697B6.129X1-Cox10tm1Ctm/J
Awaiting Transfer from the Donor
These COX10Flx mutant mice possess loxP sites flanking exon 6 of the Cox10 gene. This strain may be useful for generating conditional mutations in applications related to the study of mitochondrial myopathy and mitochondrial respiratory chain deficiency.
020422B6.C(Cg)-Grb2tm1.1Lnit/J
Awaiting Transfer from the Donor
These Grb2 floxed mice may be useful for studying the effect of GRB2-dependant signal transduction on lymphocyte differentiation and immune response.
024473B6.Cg-Fbxw11tm1.1Ybn Btrctm1Paga/GhoJ
Awaiting Transfer from the Donor
These β-TrCP2f β-TrCP1- mice harbor two mutations; the β-TrCP2 floxed allele (Fbxw11tm1Ybn) and the β-TrCP1 knockout allele (Btrctm1Paga). These mice are a Cre recombinase-inducible double knockout of β-TrCP function and allow study of multiple cellular processes (Wnt pathway, NF-κB pathway, cell cycle regulation, DNA damage response) and cancer.
024511B6.Cg-Tcf12tm3Zhu Tcf3tm4Zhu/J
Awaiting Transfer from the Donor
HEBf E2Af mice allow Cre-recombinase inducible deletion of the two basic helix-loop-helix (bHLH) E-protein family genes HEB and E2A. These mice may be useful for studying B and T lymphocyte development and lymphoid malignancies.
023528B6;129S-Igs7tm78.1(tetO-VSFPB1.2)Hze/J
Awaiting Transfer from the Donor
Ai78(TITL-VSFPB)-D (also called Ai78D) mice are a Cre/Tet-dependent, voltage-sensitive, fluorescent line utilizing an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated (TIGRE). After removal of the floxed-STOP cassette by Cre recombinase, they may be used to generate Tet-Off/Tet-On mutant animals with conditional (inducible/reversible) expression of the voltage-sensitive fluorescent protein Butterfly 1.2 (VSFP-Butterfly1.2 or VSFPB1.2). VSFP-Butterfly1.2 exhibits voltage-dependent FRET upon membrane depolarization, and enables optical recordings at high spatial resolution with intrinsic assignment of recorded signals to VSFP-targeted cell populations (such as layer 2/3 pyramidal cells).
023529B6;129S-Igs7tm79.1(tetO-hop/EGFP)Hze/J
Awaiting Transfer from the Donor
Ai79(TITL-Jaws)-D (also called Ai79D) mice are a Cre/Tet-dependent, optogenetic line utilizing an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated (TIGRE). Following Cre-mediated removal of the STOP cassette, they may be used to generate Tet-Off/Tet-On mutant animals with conditional (inducible/reversible) expression of an improved Halorhodopsin/EGFP fusion protein (Halo57*K200R*W214F/EGFP or Jaws/EGFP). Subsequent illumination of Jaws-expressing (EGFP fluorescent) cells with yellow-to-red light leads to reversible photoinhibition of action potential firing/neural activity in these cells.
023532B6;129S-Igs7tm82.1(tetO-EGFP)Hze/J
Awaiting Transfer from the Donor
Ai82(TITL-EGFP)-D (also called Ai82D) mice are a Cre/Tet-dependent, fluorescent reporter line utilizing an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated (TIGRE). After removal of the floxed-STOP cassette by Cre recombinase, they may be used to generate Tet-Off/Tet-On mutant animals with conditional (inducible/reversible) expression of EGFP.
024100B6;129S-Igs7tm90.1(tetO-COP4*/EGFP)Hze/J
Awaiting Transfer from the Donor
Ai90(TITL-Chronos)-D (also called Ai90D) mice are a Cre/Tet-dependent, optogenetic line utilizing an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated (TIGRE). Following Cre-mediated removal of the STOP cassette, they may be used to generate Tet-Off/Tet-On mutant animals with conditional (inducible/reversible) expression of the channelrhodopsin/EGFP fusion protein (Chronos/EGFP). Subsequent illumination of Chronos-expressing (EGFP fluorescent) cells with blue-to-green light leads to reversible photostimulation of action potential firing/neural activity in these cells.
025330B6N.129P2(Cg)-Igs13tm1Dolm Igs14tm1Dolm/J
Awaiting Transfer from the Donor
A copy number variation on human chromosome 16p11.2 is among the most common genetic variations found in autism spectrum disorders. 16p11flx mice are a Cre or FLP recombinase-inducible mouse model of 16p11.2 deletion that has several loxP and frt sites flanking the corresponding 440 kbp region on mouse chromosome 7F3. These mice may be useful in studying basal ganglia circuitry and the pathophysiology of autism.
025629B6N.129P2-Cx3cr1tm3(DTR)Litt/J
Awaiting Transfer from the Donor
Cre excision of a floxed stop cassette in these Cx3cr1 knockin mice enables selective depletion of associated cells upon administration of diphtheria toxin.
025396B6N.129S1(Cg)-Hhextm2Cwb/J
Awaiting Transfer from the Donor
Hhexflox/flox mice may be useful for studying hepatoblast differentiation and intrahepatic bile duct morphogenesis.
023520C57BL/6-Bcrtm1(BCR/ABL)Tsr/J
Awaiting Transfer from the Donor
These conditional knock-in/knockout mice constitutively express a single copy of the human BCR/ABL p210-IRES-EGFP oncogene from the endogenous mouse Bcr locus after the excision of a loxP-flanked Stop cassette. Crosses with hematopoietic-specific cre lines produce animals that develop the earliest stages of CML, but not leukemia.
024282C57BL/6-Cd28tm1Ltu/J
Awaiting Transfer from the Donor
These floxed mutant mice possess loxP sites flanking exons 2 and 3 of the Cd28 gene. This strain may be useful for generating conditional mutations in applications related to T cell activation, proliferation, and survival.
023974NOD.129(Cg)-Tg(CAG-Bgeo/ALPP)1Lbe/SbwJ
Awaiting Transfer from the Donor
This strain is a double reporter system, with lacZ expression being replaced with alkaline phosphatase expression in tissues when bred to Cre-expressing mice.
025534STOCK Etv5tm1.1Xsun/J
Awaiting Transfer from the Donor
Etv5fl/fl floxed mutant mice may be useful for studying Etv5 transcriptional control during cell growth and differentiation, and stem cell self-renewal.
025615STOCK Islr2tm2.1Ddg/J
Awaiting Transfer from the Donor
A portion of exon 3 of the mouse Islr2 (Linx) gene is flanked by loxP sites in this targeted mutant strain. Cre excision of the floxed region results in a knockout of the gene.
024941STOCK Lats1tm1.1Jfm/RjoJ
Awaiting Transfer from the Donor
These Lats1f/f floxed mice may be useful for studying the role Lats1 in preventing the downregulation of cellular proliferation and organ growth by the Hippo pathway.
025428STOCK Lats2tm1.1Jfm/RjoJ
Awaiting Transfer from the Donor
These Lats2f/f floxed mice may be useful for studying the role Lats2 in preventing the downregulation of cellular proliferation and organ growth by the Hippo pathway.
025417STOCK Megf8tm1.2Ddg/J
Awaiting Transfer from the Donor
This strain carries a floxed allele of Megf8, identified as a novel modifier of BMP4 (bone morphogenetic protein 4) signaling in trigeminal ganglion (TG) neurons. Cre excision of the floxed region creates a knockout of the gene.

(29 stocks)         Back to Top

New Strains Awaiting Transfer from the Donor
  • Receive periodic updates on the status of the colony AWAITING TRANSFER
  • Obtain advance notification of strain availability and opportunity to order prior to the strain being published as available
  • Provide input affecting speed and quantity of availability
The Jackson Laboratory serves as a worldwide distributor and national repository for common and rare strains of inbred mice and mice carrying spontaneous mutations or induced mutations (i.e., transgenic, targeted/"knockout", or chemically induced mutations). At any one time, we have over 100 strains at various stages of development and colony expansion.

It is VERY IMPORTANT that you register interest in strains Awaiting Transfer. The anticipated demand for a strain enables us to determine effectively the distribution plan for each strain. Registering interest also provides benefits to you (including advance notification of pending availability). Whether a strain is made available from a live colony OR from our cryopreservation repository, you may want to consider the option of Dedicated Supply. To learn more about Dedicated Supply, go to Services.

Additional Register Interest Strains

Please indicate your interest in purchasing any of the strains listed below when they become available for distribution by checking the box next to the strain(s) of interest and then selecting the "Continue" button which leads to an Interest Form.

View a Data sheet for a Strain
Select the strain name to link to the strain data sheet.

Stock
Number
Strain Name
 
Strain Description
Standard Supply
025532129S.Cg-Crebbptm1Jvd/J
In Progress
Exon 9 of the mouse Crebbp gene is flanked by loxP sites in this conditional mutant strain that is useful in studies of tumor suppression and transcriptional regulation. Cre-mediated excision of the floxed region results in a knockout allele.
018451129S1/SvImJ-Artm2.1Reb/J
In Progress
Exon 1 of the X-linked androgen receptor (Ar) gene is floxed, making this conditional mutant strain useful when studying male sexual development and fertility.
018450B6.129S1-Artm2.1Reb/J
In Progress
Exon 1 of the X-linked androgen receptor (Ar) gene is floxed, making this conditional mutant strain useful when studying male sexual development and fertility.
022761B6.Cg-Zfp335tm1.1Caw/J
In Progress
Zfp335 (zinc finger protein 335; also known as identified as a NRC-interacting factor 1 (Nif1)) is a regulator of vertebrate neurogenesis. A c.3332g>a mutation in the human gene is associated with severe microcephaly, neuronal degeneration, and neonatal death. The mouse Zfp335 promoter, exon 1, and exon 2 are flanked by loxP sites in this conditional targeted mutation strain, enabling tissue-specific knockouts mediated by Cre recombinase.
025168B6J.129P2-Ep300tm2Pkb/J
In Progress
These floxed mutant mice possess loxP sites flanking exon 9 of the Ep300 gene. This strain may be useful for generating conditional mutations in applications related to T-cell development and hematopoiesis.
025173B6J.129S6-Med23tm1.1Pkb/J
In Progress
These floxed mutant mice possess loxP sites flanking exons 5, 6 and 7 of the Med23 gene. This strain may be useful for generating conditional mutations in applications related to T-cell development and function, and activator-dependent transcription.
025178B6J.Cg-Crebbptm1Jvd/J
In Progress
These floxed mutant mice possess loxP sites flanking exon 9 of the Crebbp gene. This strain may be useful for generating conditional mutations in applications related to transcriptional regulation, chromatin remodeling, hematopoiesis, formation of short- and long-term memory, T cell development and tumor suppression.
023537B6N.129S6-Gt(ROSA)26Sortm1(CAG-tdTomato*,-EGFP*)Ees/J
In Progress
These two-color fluorescent Cre reporter mice express nuclear-localized red fluorescence in a widespread fashion prior to Cre recombinase exposure. Following exposure to Cre, nuclear-localized green fluorescence is observed. These mice function similarly to ROSAmT/mG mice (Stock Nos. 007576 and 007676) which direct fluorescent proteins to the cell membrane instead of the nucleus.
023842STOCK Fzd3tm2.1Nat/J
In Progress
The largest coding exon of the Fzd3 (frizzled homolog 3 (Drosophila)) gene is flanked by loxP sites and tagged by hemagglutinin in this conditional mutant strain.
024665B6.Cg-Tbx20tm1.2Rph/J
Under Development for Cryo
Tbx20f floxed mice possess loxP sites flanking exons 1-3 of the T-box 20 gene. This strain may be useful for studying congenital heart disease and adult cardiomyopathies.
023162B6;129-Zic3tm2.1Jwb/J
Under Development for Cryo
These floxed mutant mice possess loxP sites flanking exon 1 of the X-linked Zic3 gene and may be useful in applications related to the study of embryonic development.

(11 stocks)         Back to Top


It is VERY IMPORTANT that you Register Interest. The anticipated demand for a strain enables us to determine effectively the distribution plan for each strain.

Send questions to our Technical Support team using the Express Technical Support Form.
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