Search Criteria: Research Area is "Research Tools: Cre-lox System (loxP-flanked Sequences: Test/Reporter)"
| Stock Number |
Strain Name Strain Description |
Standard Supply |
| 003474 | B6.129S4-Gt(ROSA)26Sortm1Sor/J | Level 4 |
| Homozygous mice are viable and fertile, with a loxP-flanked DNA STOP sequence preventing expression of the downstream lacZ gene. When crossed with a cre transgenic strain, the STOP sequence is removed and lacZ is expressed in cells/tissues where cre is expressed. These mutant mice may be used as a Cre-reporter strain; to test the tissue/cellular expression pattern of cre transgenic mice. | ||
| 006053 | 129-Gt(ROSA)26Sortm1Luo/J | Repository- Live |
| MADM-GG mice are viable with no gross behavioral or observable abnormalities. Regardless of Cre-recombination, these mice express EGFP as their N- and C-terminal coding sequences are interrupted by the beta-actin intron in-frame. High EGFP expression in every cell can be visualized in vivo and in fixed samples. These mutant mice are a control EGFP-expressing strain for use with MADM (mosaic analysis with double markers) mice (see Stock No. 006041 [MADM-GR (EGFP/Dsred2)] and Stock No. 006067 [MADM-RG (Dsred2/EGFP)]). Using the MADM system, a researcher can generate genetic mosaics in which an individual organism contains somatic cells of different genotypes. This allows the researcher to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation and mitosis.
Mice harbor
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| 006067 | 129-Gt(ROSA)26Sortm2Luo/J | Repository- Live |
| MADM-RG mice are viable with no gross behavioral or observable abnormalities. Homozygous females produce less pups to weaning age compared to heterozygotes. These mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to mice harboring a reciprocal mutation at the same locus (see Stock No. 006041 or Stock No. 006075, MADM-GR (EGFP/Dsred2)). The resulting offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous") and must next be bred to a Cre-expressing strain for fluorescent protein expression. Prior to Cre-recombination, double mutant mice do not have colored cells: the chimeric genes do not produce functional proteins because their coding sequences are interrupted by the beta-actin intron in different reading frames. After DNA replication (G2 phase) in double mutant mice, Cre-recombinase introducti
..... For more information please see the full descriiption on the strain data sheet | ||
| 007676 | B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J | Repository- Live |
| Mice homozygous for this mT/mG mutation are viable and fertile. These mice possess loxP sites on either side of a membrane-targeted tdTomato (mT) cassette and express strong red fluorescence in all tissues and cell types examined. Tail or whole body epifluorescence is sufficient to identify mT/mG homozygotes. When bred to Cre recombinase expressing mice, the resulting offspring have the mT cassette deleted in the cre expressing tissue(s), allowing expression of the membrane-targeted EGFP (mG) cassette located just downstream. The donating investigator reports that the ACTB promoter allows stronger and persistent expression of the fluorescent proteins (especially in adult cells) compared to the endogenous Gt(ROSA) locus alone. This double-fluorescent system allows direct live visualization of both recombined and non-recombined cells at single cell resolution, offering an internal control for phenotypic analysis of Cre-induced mosaic mutants and providing a second marker fo
..... For more information please see the full descriiption on the strain data sheet | ||
| 004178 | B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J | Repository- Live |
| These Z/EG transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being liver and lung tissue. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with enhanced GFP expression in tissues expressing Cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells.
As an example, when crossed to a strain expressing Cre recombinase in olfactory sensory neurons (see Stock No. 006668), this mutant mouse strain may be useful in lineage tracing. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved t
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| 006080 | B6.129-Gt(ROSA)26Sortm2Luo/J | Repository- Live |
| MADM-RG mice are viable with no gross behavioral or observable abnormalities. Homozygous females produce less pups to weaning age compared to heterozygotes. These mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to mice harboring a reciprocal mutation at the same locus (see Stock No. 006041 or Stock No. 006075, MADM-GR (EGFP/Dsred2)). The resulting offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous") and must next be bred to a cre-expressing strain for fluorescent protein expression. Prior to Cre recombination, double mutant mice do not have colored cells: the chimeric genes do not produce functional proteins because their coding sequences are interrupted by the beta-actin intron in different reading frames. After DNA replication (G2 phase) in double mutant mice, Cre recombinase int
..... For more information please see the full descriiption on the strain data sheet | ||
| 006075 | B6.129-Gt(ROSA)26Sortm3Luo/J | Repository- Live |
| MADM-GR mice are viable with no gross behavioral or observable abnormalities. Homozygous mice have low fertility, while heterozygous mice have no reported fertility defects. These mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to mice harboring a reciprocal mutation at the same locus (see Stock No. 006067 or Stock No. 006080, MADM-RG (Dsred2/EGFP)). The resulting offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous") and must next be bred to a Cre-expressing strain for fluorescent protein expression. Prior to Cre-recombination, double mutant mice do not have colored cells: the chimeric genes do not produce functional proteins because their coding sequences are interrupted by the beta-actin intron in different reading frames. After DNA replication (G2 phase) in double mutant mice, Cre-recom
..... For more information please see the full descriiption on the strain data sheet | ||
| 006148 | B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J | Repository- Live |
| Mice that are homozygous for the mutant allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice contain an Enhanced Yellow Fluorescent Protein gene (EYFP, Clonetech) inserted into the Gt(ROSA)26Sor locus. Expression of EYFP is blocked by an upstream loxP-flanked STOP sequence. When bred to mice with a cre recombinase gene under the control of a promoter of interest, the STOP sequence of the targeted gene is deleted in the tissue of interest, and EYFP expression is observed. These mutant mice may be useful in monitoring the Cre expression in living tissues, and tracing the lineage of such cells in embryos, young, and adult mice at desired time points.
Mutant mice maintained on a genetic background containing a contribution from C57BL/6J have the potential to harbor a loss-of-function mutation in the nicotinamide (NAD) nucleotide transhydrogenase gene (Nnt, Chromosome 13). This
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| 005670 | B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J | Repository- Live |
| Homozygous mutant mice are viable, fertile, normal in size and do not display any behavioral abnormalities. When these gene targeted mice are bred to transgenic strains expressing Cre recombinase, functional rtTA and EGFP activity is observed in the double mutant offspring in the tissues that express cre. These double mutant mice may be bred to transgenic strains carrying genes of interest under the regulation of tetracycline responsive elements (TRE; tetO) to generate triple mutant mice in which the tissue specificity of the cre-transgenic line and doxycycline inducibility of the rtTA/TRE-controlled transgenes can be combined to regulate expression of the target gene. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify th
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| 007901 | B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J | Repository- Live |
| These Thy1-Brainbow 1.0 (line H) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Prior to Cre-mediated recombination, the fluorescent protein immediately adjacent to the promoter, tdimer2(12) (RFP), is expressed in peripheral and central neurons. When bred to Cre recombinase expressing mice, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): tdimer2(12) (RFP) (no recombination), mYFP, or mCerulean (CFP). A palmitoylation sequence tethers the mYFP and mCerulean (CFP) to the membrane, allowing clear labeling of axonal processes, while cytoplasmic tdimer2(12) (RFP) better labeled neuronal cell bodies and dendrites. Integration of tandem transgen
..... For more information please see the full descriiption on the strain data sheet | ||
| 007911 | B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J | Repository- Live |
| These Thy1-Brainbow 1.1 (line M) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Although the fluorescent protein immediately adjacent to the promoter, Kusabira-Orange (kOFP), was designed to be expressed prior to Cre-mediated recombination, basal kOFP expression is not observed in mouse tissues. When bred to Cre recombinase expressing mice, however, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): mCerulean (CFP), mYFP, or mCherry (RFP). The resulting fluorescent protein expression is observed in astrocytes of all areas of the brain and spinal cord, as well as dentate gyrus granule cells. A palmitoylation sequence tethers the mCherry (RFP),
..... For more information please see the full descriiption on the strain data sheet | ||
| 007921 | B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J | Repository- Live |
| These Thy1-Brainbow 2.1 (line R) transgenic mice are viable and fertile. The mice possess two invertible DNA segments (four fluorescent protein sequences in total) uniquely positioned in tandem and flanked with LoxP sites to generate a larger number of recombination outcomes; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Although the fluorescent protein immediately adjacent to the promoter, hrGFPII (with nuclear localization signal), was designed to be expressed prior to Cre-mediated recombination, basal hrGFPII expression may not be observed in mouse tissues. When bred to Cre recombinase expressing mice, however, the resulting offspring can have one of three different inversions for each transgene in each cell of the cre expressing tissue(s). In addition, two excision events may reduce the construct to one of two single invertible DNA segments which can continue to invert as long as cre is present. These different recomb
..... For more information please see the full descriiption on the strain data sheet | ||
| 003504 | B6;129-Gt(ROSA)26Sortm1Sho/J | Repository- Live |
| Mice with the Gtrosa26tm1Sho targeted mutation are similar to the B6;129-Gtrosa26tm1Sor from Dr. Philippe Soriano, but reported in the literature to be an improved reporter strain for monitoring cre-mediated excisions. The B-galactosidase-neomycin phosphotransferase fusion gene (Bgeo)-trapped reverse orientation splice acceptor Bgeo line 26 (ROSA26) locus was modified by gene targeting such that Bgeo is expressed only after Cre-mediated excision of loxP-flanked DNA sequences. Bgeo from the excised ROSA26 allele is expressed ubiquitously in embryos and adult mice. When mating the reporter strain with cre-expressing transgenic mice, one can see that the loxP-flanked ROSA26 allele is accessible to cre during early embryogenesis, as well as in a specific hematopoietic lineage (T lymphocytes). These mice may prove useful in the study of cell fate and cell migration during embryogenesis through recombinase-activated taggi
..... For more information please see the full descriiption on the strain data sheet | ||
| 004077 | B6;129-Gt(ROSA)26Sortm2Sho/J | Repository- Live |
| These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted into the Gt(ROSA)26Sor locus. Expression of the EGFP gene is blocked by a loxP-flanked STOP fragment placed between the EGFP sequence and the Gt(ROSA)26Sor promoter. This strain serves as a reporter strain, with successful Cre excision being indicated by EGFP expression in cre-expressing tissues. Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The donating investigator indicates that the EGFP expression level in this reporter strain is suitable for applications involving FACS but is too low for histological applications. | ||
| 008303 | B6;129S4-Tcfe2atm4Zhu/J | Repository- Live |
| These mice possess loxP sites on either side of the exons of the targeted gene that encode the bHLH region of the E12 and E47 isoforms. Transcription stop sequence and sequence encoding IRES-beta-galactosidase was inserted downstream of the loxP site flanked sequence. This strain can serve as a reporter strain, with successful Cre excision being indicated by beta-galactosidase expression in Cre-expressing tissues. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the exons encoding the bHLH region of the E12 and E47 isoforms deleted and beta-galactosidase expressed in the cre-expressing tissue(s). | ||
| 006465 | B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J | Repository- Live |
| These ZW transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread, but mosaic, throughout the central and peripheral nervous systems. Purkinje cells display intense beta-galactosidase activity. Approximately 50% of the total neuron population express the transgene, as detected by beta-galactosidase activity. Newborn mice exhibit widespread beta-galactosidase activity. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with wheat germ agglutinin (plant lectin) expression in tissues expressing cre. The double reporter system makes it possible to distinguish a lack of reporter (lacZ) expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity at the individual cell level. This transgenic mouse strain may be useful in tracing transneuronal or trans-synaptic connections and circuits in brain regions or in the s
..... For more information please see the full descriiption on the strain data sheet | ||
| 007910 | B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J | Repository- Live |
| These Thy1-Brainbow 1.0 (line L) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Prior to Cre-mediated recombination, the fluorescent protein immediately adjacent to the promoter, dTomato (RFP), is expressed in peripheral and central neurons. When bred to Cre recombinase expressing mice, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): dTomato (RFP) (no recombination), mCerulean (CFP), or mYFP. Integration of tandem transgene copies yields combinatorial fluorescent protein expression in each cell, and thus many possible cell colors, providing a way to distinguish adjacent neurons and visualize other cellular interactions. Of note, the singl
..... For more information please see the full descriiption on the strain data sheet | ||
| 005125 | FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J | Repository- Live |
| These mice contain the firefly luciferase (luc) gene inserted into the Gt(ROSA)26Sor locus. Expression of the luciferase gene is blocked by a loxP-flanked STOP fragment placed between the luc sequence and the Gt(ROSA)26Sor promoter. This strain serves as a Cre reporter strain. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision is indicated by luciferase expression in Cre-expressing tissues. Mice that are heterozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. | ||
| 006331 | STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J | Repository- Live |
| Homozygous "ROSA26-eGFP-DTA" mice are viable, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator reports that some homozygous males are subfertile. Mutant mice display widespread expression of EGFP, but DTA transcription is prevented by a strong transcriptional stop sequence. When bred to mice that express Cre recombinase under the control of a promoter of interest, the loxP-flanked EGFP and stop sequence are removed, and DTA expression is activated, resulting in the specific ablation of cre-expressing cells. This strain may be useful on its own as a fluorescent reporter or in combination with cre-expressing mice to produce conditional deletions of specific groups of cells. Transgenic mice also may have applications in toxicology and protein synthesis research. | ||
| 005130 | STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J | Repository- Live |
| These mice contain an Enhanced Yellow Fluorescent Protein/Smoothened homolog (Drosophila) fusion gene inserted into the Gt(ROSA)26Sor locus. The mutant allele consists of a fusion product involving Enhanced Yellow Fluorescent Protein (EYFP) and the constitutively active W539L point mutation of the mouse smoothened homolog (Drosophila) gene (SmoM2). Expression of the Smo/EYFP fusion gene is blocked by a loxP-flanked STOP fragment placed between the Gt(ROSA)26Sor promoter and the Smo/EYFP sequence. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision results in the constitutive expression of mouse smoothened homolog (Drosophila) and unrestrained Hedgehog signaling in Cre-expressing tissues. Expression of the SmoM2 fusion protein can be monitored using EYFP-specific fluorescence protocols. Mice that are homozygous for the mutant allele are viable, fertile, normal in size and do not display any gross physical
..... For more information please see the full descriiption on the strain data sheet | ||
| 005572 | STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J | Repository- Live |
| Homozygous mutant mice are viable, fertile, normal in size and do not display any behavioral abnormalities. When these gene targeted mice are bred to transgenic strains expressing Cre recombinase, functional rtTA and EGFP activity is observed in the double mutant offspring in the tissues that express cre. These double mutant mice may be bred to transgenic strains carrying genes of interest under the regulation of tetracycline responsive elements (TRE; tetO) to generate triple mutant mice in which the tissue specificity of the Cre-transgenic line and the doxycycline inducibility of the rtTA/TRE-controlled transgenes can be combined to regulate expression of the target gene. Of note, mutant mice are also available on a C57BL/6J genetic background (see Stock No. 005670). | ||
| 007576 | STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J | Repository- Live |
| Mice homozygous for this mT/mG mutation are viable and fertile. These mice possess loxP sites on either side of a membrane-targeted tdTomato (mT) cassette and express strong red fluorescence in all tissues and cell types examined. Tail or whole body epifluorescence is sufficient to identify mT/mG homozygotes. When bred to Cre recombinase expressing mice, the resulting offspring have the mT cassette deleted in the cre expressing tissue(s), allowing expression of the membrane-targeted EGFP (mG) cassette located just downstream. The donating investigator reports that the ACTB promoter allows stronger and persistent expression of the fluorescent proteins (especially in adult cells) compared to the endogenous Gt(ROSA) locus alone. This double-fluorescent system allows direct live visualization of both recombined and non-recombined cells at single cell resolution, offering an internal control for phenotypic analysis of Cre-induced mosaic mutants and providing a second marker fo
..... For more information please see the full descriiption on the strain data sheet | ||
| 005438 | STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J | Repository- Live |
| While mice hemizygous for this Z/RED transgene are reported to be viable and fertile, it has been our experience at The Jackson Laboratory that hemizygous animals are often smaller than littermates and subject to postnatal mortality. Delayed weaning greatly enhances the survival. Although homozygous animals are born, animals have not survived past five weeks of age. These transgenic mice express beta-galactosidase under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with red fluorescent protein (DsRed*MST) expression in tissues expressing Cre recombinase. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells. | ||
| 003920 | STOCK Tg(CAG-Bgeo/GFP)21Lbe/J | Repository- Live |
| These Z/EG transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being liver and lung tissue. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with enhanced GFP expression in tissues expressing cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess cre excision activity in live animals and cells. Although homozygotes are viable, attempts to breed homozygous mice proved unsuccessful. | ||
| 006041 | 129-Gt(ROSA)26Sortm3Luo/J | Repository-Cryopreserved |
| MADM-GR mice are viable with no gross behavioral or observable abnormalities. Homozygous mice have low fertility, while heterozygous mice have no reported fertility defects. These mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to mice harboring a reciprocal mutation at the same locus (see Stock No. 006067 or Stock No. 006080, MADM-RG (Dsred2/EGFP)). The resulting offspring have one copy of each reciprocal mutation on homologous chromosomes ("trans-heterozygous") and must next be bred to a Cre-expressing strain for fluorescent protein expression. Prior to Cre-recombination, double mutant mice do not have colored cells: the chimeric genes do not produce functional proteins because their coding sequences are interrupted by the beta-actin intron in different reading frames. After DNA replication (G2 phase) in double mutant mice, Cre-recom
..... For more information please see the full descriiption on the strain data sheet | ||
| 003310 | 129S-Gt(ROSA)26Sortm1Sor/J | Repository-Cryopreserved |
| Mice heterozygous or homozygous for the Gtrosa26tm1Sor targeted mutation may be used to test the tissue/cellular expression pattern of the cre transgene in any transgenic strain carrying cre under the regulation of a specific promoter. Cre expression results in the removal of a loxP-flanked DNA segment that prevents expression of a lacZ gene. When crossed with a cre transgenic strain, lacZ is expressed in cells/tissues where cre is expressed. The 129-Gtrosa26tm1Sor strain is particularly useful for this purpose because the ROSA26 promoter leads to generalized expression of lacZ during development or in the adult. | ||
| 006071 | B6.129-Gt(ROSA)26Sortm1Luo/J | Repository-Cryopreserved |
| Homozygous and heterozygous mutant mice are viable and fertile, and manifest no gross behavioral or phenotypic abnormalities. These mutant mice carry an EGFP construct in which the N- and C-terminal coding sequences are interrupted by the beta-actin intron in-frame. Despite the presence of this intron, high EGFP expression in every cell can be visualized in vivo and in fixed tissues.
These mutant mice were designed as an EGFP-expressing control strain for use with MADM (mosaic analysis with double markers) mice (See Stock No. 006041 [EGFP/Dsred2] and Stock No. 006067 [Dsred2/EGFP]). Using the MADM system, a researcher can generate genetic mosaics in which an individual organism contains somatic cells of different genotypes. This allows the researcher to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be
..... | ||
| 005630 | B6.Cg-Tg(Thy1-EYFP)15Jrs/J | Repository-Cryopreserved |
| These transgenic mice conditionally express Enhanced Yellow Fluorescent Protein (EYFP) under the direction of the mouse thymus cell antigen 1, theta, promoter. Expression of the EYFP gene is blocked by a loxP-flanked STOP fragment placed between the promoter and EYFP gene. Cre-mediated excision of the STOP cassette results in expression of EYFP in motor neurons. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. For example, when crossed to a strain with widespread expression of Cre recombinase (see Stock No. 003376), this mutant mouse strain may be useful in studies of synaptic function. | ||
| 002982 | B6.Cg-Tg(xstpx-lacZ)32And/J | Repository-Cryopreserved |
| This mutant, when crossed with a cre transgenic, will express lacZ in cells where cre is expressed to remove the STOP of translation section which lies between the 2 loxP sites. LacZ expression is restricted to neural and skeletal muscle tissue and heart by the chicken beta-actin promoter that is driving the reporter. | ||
| 003309 | B6;129S4-Gt(ROSA)26Sortm1Sor/J | Repository-Cryopreserved |
| Mice heterozygous or homozygous for the Gtrosa26tm1Sor targeted mutation may be used to test the tissue/cellular expression pattern of the cre transgene in any transgenic strain carrying cre under the regulation of a specific promoter. Cre expression results in the removal of a loxP-flanked DNA segment that prevents expression of a lacZ gene. When crossed with a cre transgenic strain, lacZ is expressed in cells/tissues where cre is expressed. | ||
| 002981 | DBA/2-Tg(xstpx-lacZ)36And/J | Repository-Cryopreserved |
| This test strain is used determine the tissue expression pattern of cre transgenic mice. The transgene is loxP-STOP-of-translation-loxP-lacZ driven by the beta-actin promoter. The lacZ is expressed only in cells where the STOP element has been removed by Cre recombinase. LacZ expression is restricted to neural and skeletal muscle tissue and heart by the chicken beta-actin promoter that is driving the reporter. | ||
| 003919 | STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J | Repository-Cryopreserved |
| These transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being erythrocytes, chondrocytes and adipocytes. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with alkaline phosphatase expression in tissues expressing Cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess cre excision activity at the individual cell level. | ||
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