Search Criteria: Research Area is "Research Tools: FLP-FRT System (FLP Recombinase Expression: Germline/Embryonic Expression)"
| Stock Number |
Strain Name Strain Description |
Standard Supply |
| 003946 | 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J | Repository- Live |
| This knock-in strain expresses a variant of the Saccharomyces cerevisiae FLP1 recombinase gene under the direction of the Gt(ROSA)26Sor promoter. The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being four-fold and ten-fold that of wild type FLP at 37C and 40C, respectively. Homozygous mice for the knock-in allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Gt(ROSA)26Sor promoter drives expression in a constitutive fashion from preimplantation onward. Extensive target gene recombination can be achieved in most tissue types, including cells of the developing germ line. This mutant strain is suitable for use as a deleter strain and serves as a source of FLP1-expressing primary cell lines. | ||
| 005703 | B6.Cg-Tg(ACTFLPe)9205Dym/J | Repository- Live |
| This transgenic strain expresses a variant of the Saccharomyces cerevisiae FLP1 recombinase gene under the direction of the human ACTB promoter. The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being four-fold and ten-fold that of wildtype FLP at 37oC and 40oC, respectively. Mice that are hemizygous for the transgenic allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5. This deleter strain is a suitable alternative to, and complement with the Cre-loxP system for in vivo genetic engineering.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype coul
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| 003800 | B6;SJL-Tg(ACTFLPe)9205Dym/J | Repository- Live |
| This transgenic strain expresses a variant of the Saccharomyces cerevisiae FLP1 recombinase gene under the direction of the human ACTB promoter. The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being four-fold and ten-fold that of wildtype FLP at 37C and 40C, respectively. Mice that are hemizygous for the transgenic allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5. This deleter strain is a suitable alternative to, and complement with the Cre-loxP system for in vivo genetic engineering. | ||
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