Search Criteria: Research Area is "Neurobiology Research: Cre-lox System"
| Stock Number |
Strain Name Strain Description |
Standard Supply |
| 003474 | B6.129S4-Gt(ROSA)26Sortm1Sor/J | Level 4 |
| Homozygous mice are viable and fertile, with a loxP-flanked DNA STOP sequence preventing expression of the downstream lacZ gene. When crossed with a cre transgenic strain, the STOP sequence is removed and lacZ is expressed in cells/tissues where cre is expressed. These mutant mice may be used as a Cre-reporter strain; to test the tissue/cellular expression pattern of cre transgenic mice. | ||
| 004337 | 129(Cg)-Foxg1tm1(cre)Skm/J | Repository- Live |
| This strain expresses Cre recombinase from the endogenous Foxg1 locus. Forkhead box G1 is required for telencephalon development and is expressed specifically in the telencephalon and discrete head structures. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in the telencephalon, anterior optic vesicle (developing lens and retina), otic vesicle, facial and head ectoderm, olfactory epithelium, mid-hindbrain junction and pharyngeal pouches. Mice that are homozygous for the targeted mutation die perinatally. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain represents a model that may be useful in studies of telencephalic development. | ||
| 004293 | 129-Shhtm2Amc/J | Repository- Live |
| Mice that are homozygous for the Shhtm2Amc targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This conditional mutant contains two loxP sites flanking exon 2 of the targeted allele. Cre-mediated recombination excises exon 2 and some surrounding intronic sequence, generating a null allele. When the conditional mutant is crossed with a ubiquitously-expressing Cre recombinase carrier to remove Shh activity in the early embryo, the resulting phenotype resembles the Shh null mutation. These conditional mutant mice may be mated to strains expressing Cre recombinase to study the effects of temporal and tissue-specific ablation of the targeted allele. This mutant mouse strain represents a model that may be useful in studies of developmental defects resulting from disruption of Shh-dependent pathways.
When bred to a strain expressing Cre recombinase under the control of a tet
..... | ||
| 008002 | 129S-Pafah1b1tm2Awb/J | Repository- Live |
| These mice possess loxP sites flanking exons 3 through 6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Histological analysis of brains from homozygotes reveals slightly wider CA1 and CA3 regions and a split in CA2 region in the hippocampus. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3 through 6 deleted in the cre-expressing tissue(s). | ||
| 008118 | B6(Cg)-Snord116tm1Uta/J | Repository- Live |
| Mice homozygous for this 2-loxP (floxed) allele are viable and fertile, with loxP sites flanking the Snord116 small nucleolar RNAs (snoRNAs) gene cluster. When bred to mice that express Cre recombinase, the resulting offspring will have this gene cluster deleted in the cre-expressing tissue(s). Because the Snord116 gene cluster is imprinted and only expressed from the paternal allele, breeding 2-loxP males with cre-expressing females may be required to generate deleted offspring with the knockout phenotype. The donating investigator reports that the distance between the two loxP sites (~140 kb) may reduce the recombination efficiency in somatic cells. As deletions of the Snord116 cluster are associated with Prader-Willi syndrome (PWS), mice carrying the 2-loxP (floxed) allele may be useful in generating conditional mutations for studying the role of Snord116 in growth and feeding regulation, mechanisms of obesity, and patho
..... For more information please see the full descriiption on the strain data sheet | ||
| 007676 | B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J | Repository- Live |
| Mice homozygous for this mT/mG mutation are viable and fertile. These mice possess loxP sites on either side of a membrane-targeted tdTomato (mT) cassette and express strong red fluorescence in all tissues and cell types examined. Tail or whole body epifluorescence is sufficient to identify mT/mG homozygotes. When bred to Cre recombinase expressing mice, the resulting offspring have the mT cassette deleted in the cre expressing tissue(s), allowing expression of the membrane-targeted EGFP (mG) cassette located just downstream. The donating investigator reports that the ACTB promoter allows stronger and persistent expression of the fluorescent proteins (especially in adult cells) compared to the endogenous Gt(ROSA) locus alone. This double-fluorescent system allows direct live visualization of both recombined and non-recombined cells at single cell resolution, offering an internal control for phenotypic analysis of Cre-induced mosaic mutants and providing a second marker fo
..... For more information please see the full descriiption on the strain data sheet | ||
| 004178 | B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J | Repository- Live |
| These Z/EG transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being liver and lung tissue. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with enhanced GFP expression in tissues expressing Cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells.
As an example, when crossed to a strain expressing Cre recombinase in olfactory sensory neurons (see Stock No. 006668), this mutant mouse strain may be useful in lineage tracing. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved t
..... | ||
| 006874 | B6.129-Gabra4tm1.2Geh/J | Repository- Live |
| Mice homozygous for this GABAA-R alpha4F allele are viable and fertile. These mutant mice have loxP sites flanking exon 3 of the targeted gene. When bred to Cre-recombinase expressing mice, offspring will have a deletion of exon 3 in the cre expressing tissue(s). These "floxed" mice may be useful in neurological studies including behavior and neurotransmitter function. Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations are available from this donating investigator (Dr. Gregg Homanics, University of Pittsburgh), including Gabra1 (Stock No. 004318), Gabra4 (Stock No. 006874), Gabra6 (Stock No. 002710), Gabrb3 (Stock No. 002711), Gabrd (Stock No.
..... | ||
| 008320 | B6.129-Leprtm2(cre)Rck/J | Repository- Live |
| Mice hemizygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Cre activity is demonstrable in the hypothalmus (arcuate, dorsomedial (DMH), lateral (LH), and ventromedial (VMH) nuclei), limbic and cortical brain regions (basolateral amygdaloid nucleus (BLA), piriform cortex (Pir), and lateral entorhinal cortex (LEnt)), and retrosplenial cortex. When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked genome in tissues that normally express the gene. This strain has been used in virus-assisted mapping of neural inputs and may be useful in studies of neural features of feeding behaviors. | ||
| 006146 | B6.129-Smn1tm1Jme/J | Repository- Live |
| Mice homozygous for this SMNF7 floxed allele are viable and fertile and do not display any gross physical or behavioral abnormalities. Mutant mice exhibit no transcript splicing defects. Cre-mediated recombination of the loxP-flanked sequences results in deletion of exon 7 of the targeted gene. As mutations of this exon are implicated in 95% of all human spinal muscular atrophy (SMA), these mice may be useful in studying SMA or other neuromuscular degenerative diseases.
When crossed to a strain expressing Cre recombinase in neurons (see Stock No. 005938, Stock No. 006297, and Stock No. 006663), this mutant mouse strain may be useful as a model of SMA. When crossed to a strain expressing Cre recombinase in striated muscle fibers (see Stock No. For more information please see the full descriiption on the strain data sheet | ||
| 004146 | B6.129-Tg(Pcp2-cre)2Mpin/J | Repository- Live |
| These transgenic mice express a cre gene inserted into exon 4 of a Pcp2 gene. Mice homozygous for the insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity is observed in most Purkinje cells and some retinal bipolar neurons. Small amounts of activity are observed in an unidentified population of cells of the central nervous system tissue. Recombination is first observed around postnatal day 6 and is fully established 2 to 3 weeks after birth.
View cre expression characterization. | ||
| 006084 | B6.129P2(Cg)-Foxg1tm1(cre)Skm/J | Repository- Live |
| This strain expresses Cre recombinase from the endogenous Foxg1 locus. Forkhead box G1 is required for telencephalon development and is expressed specifically in the telencephalon and discrete head structures. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in the telencephalon, anterior optic vesicle (developing lens and retina), otic vesicle, facial and head ectoderm, olfactory epithelium, mid-hindbrain junction and pharyngeal pouches. Mice that are homozygous for the targeted mutation die perinatally. Heterozygous mutant mice are viable, fertile, normal in size. On the C57BL/6 background, forebrain volume in heterozygotes is substantially reduced especially in the cerebral cortex (40.7%), striatum (29.7%), and hippocampus (18.6%). In the adult, the thalamus is reduced in volume by 21.6%. This mutant mouse strain represents a model that ma
..... For more information please see the full descriiption on the strain data sheet | ||
| 006600 | B6.129S1-Mnx1tm4(cre)Tmj/J | Repository- Live |
| Mice heterozygous for this HB9cre targeted mutation are viable and fertile, with cre expression replacing HB9 (Hlxb9 or Mnx1) expression. Under control of the endogenous upstream elements, cre expression is directed to motor neurons. In heterozygotes, cre expression coincides with HB9 expression. Homozygous HB9cre mice die at or soon after birth, with expression of Cre recombinase likewise directed to motor neurons but no expression of endogenous HB9. When these HB9cre mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination in the resulting offspring leads to deletion of the flanked sequences in Mnx1/HB9 expressing cells; making them useful in neurodevelopmental studies of homeobox genes, motor neuron function and differentiation, and the central nervous system. | ||
| 005628 | B6.129S2-Emx1tm1(cre)Krj/J | Repository- Live |
| Mice homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain expresses Cre recombinase from the endogenous Emx1 locus. Western blot analysis of cortical brain tissue does not detect reduced endogenous gene product (protein). When crossed with a strain containing a loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in approximately 88% of the neurons of the neocortex and hippocampus, and in the glial cells of the pallium, mimicking the pattern of expression of the endogenous gene. This mutant mouse strain represents a model that may be useful in studies of forebrain development and function. | ||
| 007668 | B6.129S4(Cg)-Arntltm1Weit/J | Repository- Live |
| Mice homozygous for this conditional (floxed) allele possess loxP sites flanking exon 8 of the targeted gene and are viable and fertile, with circadian behavioral rhythms indistinguishable from wildtype littermates. When bred to mice that express Cre recombinase, the resulting offspring will have the exon encoding the ARNTL (BMAL1) basic helix-loop-helix (bHLH) domain deleted in the cre-expressing tissue(s). These Bmal1-floxed mutant mice may be useful in generating conditional mutations (whole-mouse or tissue-specific) to study the role of circadian clock/circadian rhythm in physiological and behavioral regulation.
For example, when crossed to a strain expressing Cre recombinase in the retina (see Stock No. 005105), this mutant mouse strain may be useful in studies of the circadian clock of the retina. | ||
| 006148 | B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J | Repository- Live |
| Mice that are homozygous for the mutant allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice contain an Enhanced Yellow Fluorescent Protein gene (EYFP, Clonetech) inserted into the Gt(ROSA)26Sor locus. Expression of EYFP is blocked by an upstream loxP-flanked STOP sequence. When bred to mice with a cre recombinase gene under the control of a promoter of interest, the STOP sequence of the targeted gene is deleted in the tissue of interest, and EYFP expression is observed. These mutant mice may be useful in monitoring the Cre expression in living tissues, and tracing the lineage of such cells in embryos, young, and adult mice at desired time points.
Mutant mice maintained on a genetic background containing a contribution from C57BL/6J have the potential to harbor a loss-of-function mutation in the nicotinamide (NAD) nucleotide transhydrogenase gene (Nnt, Chromosome 13). This
..... | ||
| 005359 | B6.Cg-Tg(Camk2a-cre)T29-1Stl/J | Repository- Live |
| Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the Cre recombinase under the control of the mouse calcium/calmodulin-dependent protein kinase II alpha promoter. Cre recombinase expression is detected in the forebrain, specifically to the CA1 pyramidal cell layer in the hippocampus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination occurs in the pyramidal cell layer. | ||
| 003771 | B6.Cg-Tg(Nes-cre)1Kln/J | Repository- Live |
| These transgenic mice express Cre recombinase under the control of the rat nestin promoter and enhancer. Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Initial studies utilizing a reporter strain carrying a beta galactosidase transgene whose expression is dependent on Cre-mediated recombination indicate that cre is primarily expressed in the central and peripheral nervous system with a few isolated kidney and heart cells also expressing activity. The donating investigator indicates that Cre recombinase activity is present in nervous tissue by embryonic day 11.
View cre expression characterization. | ||
| 005975 | B6.Cg-Tg(Plp1-cre/ESR1)3.16Pop/J | Repository- Live |
| These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse Plp1, proteolipid protein (myelin) 1 promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration allows for ablation of predetermined genes in oligodendrocytes and Schwann cells, and will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice. M
..... For more information please see the full descriiption on the strain data sheet | ||
| 007901 | B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J | Repository- Live |
| These Thy1-Brainbow 1.0 (line H) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Prior to Cre-mediated recombination, the fluorescent protein immediately adjacent to the promoter, tdimer2(12) (RFP), is expressed in peripheral and central neurons. When bred to Cre recombinase expressing mice, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): tdimer2(12) (RFP) (no recombination), mYFP, or mCerulean (CFP). A palmitoylation sequence tethers the mYFP and mCerulean (CFP) to the membrane, allowing clear labeling of axonal processes, while cytoplasmic tdimer2(12) (RFP) better labeled neuronal cell bodies and dendrites. Integration of tandem transgen
..... For more information please see the full descriiption on the strain data sheet | ||
| 007911 | B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J | Repository- Live |
| These Thy1-Brainbow 1.1 (line M) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Although the fluorescent protein immediately adjacent to the promoter, Kusabira-Orange (kOFP), was designed to be expressed prior to Cre-mediated recombination, basal kOFP expression is not observed in mouse tissues. When bred to Cre recombinase expressing mice, however, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): mCerulean (CFP), mYFP, or mCherry (RFP). The resulting fluorescent protein expression is observed in astrocytes of all areas of the brain and spinal cord, as well as dentate gyrus granule cells. A palmitoylation sequence tethers the mCherry (RFP),
..... For more information please see the full descriiption on the strain data sheet | ||
| 007921 | B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J | Repository- Live |
| These Thy1-Brainbow 2.1 (line R) transgenic mice are viable and fertile. The mice possess two invertible DNA segments (four fluorescent protein sequences in total) uniquely positioned in tandem and flanked with LoxP sites to generate a larger number of recombination outcomes; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Although the fluorescent protein immediately adjacent to the promoter, hrGFPII (with nuclear localization signal), was designed to be expressed prior to Cre-mediated recombination, basal hrGFPII expression may not be observed in mouse tissues. When bred to Cre recombinase expressing mice, however, the resulting offspring can have one of three different inversions for each transgene in each cell of the cre expressing tissue(s). In addition, two excision events may reduce the construct to one of two single invertible DNA segments which can continue to invert as long as cre is present. These different recomb
..... For more information please see the full descriiption on the strain data sheet | ||
| 007606 | B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J | Repository- Live |
| These transgenic mice ("SLICK" for single-neuron labeling with inducible Cre-mediated knock-out) have a tamoxifen inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted neuron-specific deletions only in neurons where the Thy1 promoter is active. These cells will be evident by the expression of Yellow Fluorescent Protein. Transgenic mice express YFP constitutively in neurons. The Donating Investigator reports that founder line A mice express the transgene in small subsets of motor neurons and dorsal root ganglion neurons.
..... For more information please see the full descriiption on the strain data sheet | ||
| 008085 | B6.Cg-Tg(UBC-cre/ESR1)1Ejb/J | Repository- Live |
| Mice hemizygous for this Cre-ERT2 transgene are viable and fertile. Mice from this founder line have strong tamoxifen-inducible cre activity in all reported tissue types. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to OHT. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in widespread cel
..... For more information please see the full descriiption on the strain data sheet | ||
| 006451 | B6.FVB(129X1)-Tg(Sim1-cre)1Lowl/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Transgene expression is observed in all areas that endogenously express Sim1, including paraventricular hypothalamus and other parts of the brain. When these Sim1-Cre mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequences in Sim1-expressing tissues (including hypothalamus). As such, Sim1-Cre transgenic mice may be useful in studying body weight homeostasis, obesity, leptin metabolism, or as a reporter strain for Sim1-transcription factor activity.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the st
..... | ||
| 003504 | B6;129-Gt(ROSA)26Sortm1Sho/J | Repository- Live |
| Mice with the Gtrosa26tm1Sho targeted mutation are similar to the B6;129-Gtrosa26tm1Sor from Dr. Philippe Soriano, but reported in the literature to be an improved reporter strain for monitoring cre-mediated excisions. The B-galactosidase-neomycin phosphotransferase fusion gene (Bgeo)-trapped reverse orientation splice acceptor Bgeo line 26 (ROSA26) locus was modified by gene targeting such that Bgeo is expressed only after Cre-mediated excision of loxP-flanked DNA sequences. Bgeo from the excised ROSA26 allele is expressed ubiquitously in embryos and adult mice. When mating the reporter strain with cre-expressing transgenic mice, one can see that the loxP-flanked ROSA26 allele is accessible to cre during early embryogenesis, as well as in a specific hematopoietic lineage (T lymphocytes). These mice may prove useful in the study of cell fate and cell migration during embryogenesis through recombinase-activated taggi
..... For more information please see the full descriiption on the strain data sheet | ||
| 005549 | B6;129-Pax3tm1(cre)Joe/J | Repository- Live |
| This strain expresses Cre recombinase from the endogenous Pax3 locus. Expression of the targeted gene product (mRNA and protein) mimics endogenous gene expression as detected by in situ hybridization and immunohistochemistry of homozygous embryos aged E12.5. No endogenous Pax3 gene product (protein) is detected in homozygotes and approximately one half of the endogenous gene product (protein) is detected in heterozygotes by Western blot analysis. Cre recombinase expression is detected in the dorsal neural tube and somites of E9 to 11.5 embryos and in the cardiac neural crest cells and colonic epithelia of E11.5 embryos. Recombination occurs in neural crest and somite derivatives of later gestation embryos. Homozygous mice have an embryonic lethal phenotype, failing to develop past embryonic day 18.5. At age E13.5 homozygous embryos display severe cardiac and neural tube defects (exencephaly), absent limb musculature and reduced or absent dorsal root ganglia. Heterozygous
..... For more information please see the full descriiption on the strain data sheet | ||
| 008294 | B6;129-Syt11tm1Sud/J | Repository- Live |
| These transgenic animals carry a FLAG-(C)4-FLAG insertion in exon 2 of the gene. Homozygotes are viable and have no obvious phenotype. Mutant protein expression levels are similar to those of the wildtype protein. Expression is brain-specific and localized in the neuronal soma and dendrites (presumably on cargo vesicles). Protein can be recognized using FLAG antibody by Western blot analysis, but not in histological sections or labeled cultures. No significant N-ethyl-maleimide-sensitive factor attachment protein receptor (SNARE) binding is observed in immunoprecipitation (IP) assays. Exon 2 is flanked by loxP sites. When crossed with a Cre recombinase-expressing strain, tissue-specific expression can be eliminated by deletion of exon 2. This strain may be useful in studies of neurotransmitter release mechanisms. | ||
| 008295 | B6;129-Syt9tm1Sud/J | Repository- Live |
| Mice homozygous for this targeted knock-in are viable and fertile and do not display any gross physical or behavioral abnormalities.Synaptotagmin IX knock-in mice contain GFP "emerald" fused to the first exon of the targeted gene, and exon 2 is flanked by loxP sites. GFP expression is primarily localized in the limbic system and striatum of the brain. When crossed with a Cre recombinase-expressing strain, offspring are produced with tissue-specific deletion of Syt9 and which lack GFP expression, suggesting this creates a null allele. This strain may be used to study Ca2+ sensors involved with fast neurotransmitter release. Synaptotagmin IX knock-in mice contain GFP " | ||
| 007605 | B6;129P-Psen1tm1Vln/J | Repository- Live |
| These mice possess loxP sites on either side of exon 7 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these "floxed" mice are bred to mice that express Cre recombinase, resulting offspring can have one of three resulting genotypes (only exon 7 deleted, only the neo selection cassette deleted, or both exon 7 and the neo selection cassette deleted) in the cre-expressing tissue(s). These PS1-floxed mice may be useful in generating conditional knockouts of Presenilin 1 for studying Alzheimer's Disease. | ||
| 006849 | B6;129P2-Mecp2tm2Bird/J | Repository- Live |
| These mice possess a loxP-flanked STOP cassette in intron 2 of the targeted gene on the X chromosome. Western blot and hybridization analysis confirm the absence of wildtype protein from the targeted allele. Hemizygous (Mecp2lox-Stop/y) males do not breed and develop Rett syndrome symptoms (reduced mobility, hindlimb clasping) at approximately 6 weeks of age, with death occurring at approximately 11 weeks of age. Heterozygous females are fertile until developing Rett syndrome characteristics at 4-12 months of age. This Rett syndrome-like phenotype is similar to that observed for the traditional knock-out allele (see Stock No. 003890). Cre recombinase-mediated removal of the floxed-STOP cassette restores transcription from the targeted allele and MECP2 protein activity to normal, and reverses the Rett syndrome-like neurological defects. This mutant mouse strain may be bred to a strain expressing tamoxi
..... | ||
| 007001 | B6;129S-Tg(UBC-cre/ESR1)1Ejb/J | Repository- Live |
| Mice hemizygous for this Cre-ERT2 transgene are viable and fertile. Mice from this founder line have strong tamoxifen-inducible cre activity in all reported tissue types. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to OHT. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in widespread cel
..... For more information please see the full descriiption on the strain data sheet | ||
| 006410 | B6;129S6-Chattm1(cre)Lowl/J | Repository- Live |
| Homozygous mice maintained at The Jackson Laboratory are viable and fertile. An "IRES-Cre" sequence is inserted downstream of the stop codon such that cre expression is controlled by the endogenous Chat gene promoter. Chat gene expression, however, is unaffected. Cre recombinase activity is reported in all cholinergic neurons. These mice may be useful for "Cre-lox" technology applications in neurobiology, including studies of motor function, learning and memory, Alzheimer's disease, and Down syndrome, and in obesity and diabetes research.
View cre expression characterization. | ||
| 006465 | B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J | Repository- Live |
| These ZW transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread, but mosaic, throughout the central and peripheral nervous systems. Purkinje cells display intense beta-galactosidase activity. Approximately 50% of the total neuron population express the transgene, as detected by beta-galactosidase activity. Newborn mice exhibit widespread beta-galactosidase activity. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with wheat germ agglutinin (plant lectin) expression in tissues expressing cre. The double reporter system makes it possible to distinguish a lack of reporter (lacZ) expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity at the individual cell level. This transgenic mouse strain may be useful in tracing transneuronal or trans-synaptic connections and circuits in brain regions or in the s
..... For more information please see the full descriiption on the strain data sheet | ||
| 007910 | B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J | Repository- Live |
| These Thy1-Brainbow 1.0 (line L) transgenic mice are viable and fertile. The mice possess multiple fluorescent protein sequences uniquely flanked with pairs of incompatible Lox sites alternated to create mutually exclusive recombination events; allowing stochastic expression of multiple fluorescent proteins from a single transgene. Prior to Cre-mediated recombination, the fluorescent protein immediately adjacent to the promoter, dTomato (RFP), is expressed in peripheral and central neurons. When bred to Cre recombinase expressing mice, the resulting offspring can have one of three expression outcomes for each transgene in each cell of the cre expressing tissue(s): dTomato (RFP) (no recombination), mCerulean (CFP), or mYFP. Integration of tandem transgene copies yields combinatorial fluorescent protein expression in each cell, and thus many possible cell colors, providing a way to distinguish adjacent neurons and visualize other cellular interactions. Of note, the singl
..... For more information please see the full descriiption on the strain data sheet | ||
| 007610 | B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J | Repository- Live |
| These transgenic mice ("SLICK" for single-neuron labeling with inducible Cre-mediated knock-out) have a tamoxifen inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted neuron-specific deletions only in neurons where the Thy1 is active. These cells will be evident by the expression of Yellow Fluorescent Protein. Transgenic mice express YFP constitutively in neurons. The Donating Investigator reports that founder line V mice express the transgene sparsely in neurons of the central nervous system.
This strain is one of
..... | ||
| 007900 | C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J | Repository- Live |
| Mice homozygous for this iDTR mutation are viable and fertile. These mice have the simian Diphtheria Toxin Receptor (DTR; from simian Hbegf) inserted into the Gt(ROSA)26Sor (ROSA26) locus. Widespread expression of DTR is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTR expression. Cells expressing DTR are rendered susceptible to ablation following Diphtheria toxin administration.
For example, when bred to a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 006785), this mutant mouse strain may be useful in studies of lymphocyte cell ablation. | ||
| 006474 | C57BL/6-Tg(Grik4-cre)G32-4Stl/J | Repository- Live |
| Mice hemizygous for this "G32-4" transgene are viable, fertile, and do not display any gross physical or behavioral abnormalities. Transgene expression (cre activity) is detectable at 14 days old in area CA3 of the hippocampus, and at 8 weeks of age, recombination is observed in nearly 100% of pyramidal cells in area CA3. Recombination is also observed in other brain areas, but at distinctly lower frequencies. If bred with mice containing a loxP-flanked sequence of interest, tissue-specific deletion of that sequence results in the offspring. Specifically, when these mice were bred with a conditional CaMKII allele (see Stock No. 006575), the resulting offspring exhibited altered neurotransmitter release. The donating investigator reports that G32-4 females may confer global Cre-mediated deletion of loxP-flanked sequences in some offspring, and recommend using male G32-4 mice exclusively for such trials. These C
..... For more information please see the full descriiption on the strain data sheet | ||
| 006481 | C57BL/6J-Tg(ACTB-NOTCH1)1Shn/J | Repository- Live |
| Transgenic mice are viable, fertile and behaviorally normal. These "CALSL-NICD (H)" mice (or simply CALSL-NICD) reportedly carry 10-20 copies of the transgene inserted into a single genomic locus. Expression of the transgene-derived intracellular domain of human NOTCH1 is prevented by a "Lox-STOP-Lox" cassette. When transgenic mice are bred to a strain expressing Cre recombinase, the "floxed stop" cassette is excised in the resulting offspring, and human NOTCH1 expression is observed in the cre-expressing tissue(s). These transgenic mice may be useful in studying early neural progenitor cell development and apoptosis, and responses to tissue-specific Notch activation. For example, when crossed to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771), this transgenic mouse strain may be useful in studies of notch signaling during apoptotic cell death. | ||
| 004600 | FVB-Tg(GFAP-cre)25Mes/J | Repository- Live |
| Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Mice that are homozygous for the transgene are not viable. This transgenic mouse strain expresses Cre recombinase under the control of the human glial fibrillary acidic protein promoter (GFAP). When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons. Expression activity is also present in periportal cells of the liver. Developmental onset of transgene expression occurs in the dorsal and medial regions of the telencephalon by embryonic day 13.5. In adult cerebellum, only astrocytes are immunoreactive for GFAP or Cre recombinase. This mutant mouse strain represents an effective tool for generating central ner
..... For more information please see the full descriiption on the strain data sheet | ||
| 006364 | FVB-Tg(Nr5a1-cre)2Lowl/J | Repository- Live |
| Mice hemizygous for the "Sf1-Cre" transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Transgene expression mimics the mRNA pattern of Nr5a1; with Cre activity observed in steroidogenic factor-1 (SF1)-positive neurons in the ventromedial hypothalamic nucleus (VMH) as well as pituitary, gonad, and adrenal tissue. Expression is also noted in the cerebral cortex and in a few scattered cells in the caudal brainstem of mice derived from line 2 (but not line 7). If bred with mice containing a loxP-flanked sequence of interest, tissue-specific deletion of that genome results in the offspring. Specifically, these cre-expressing mice may be useful in studies involving the hypothalamus, such as body weight homeostasis, obesity, leptin metabolism, or as a reporter strain for SF1-transcription factor activity. | ||
| 006138 | FVB.129(B6)-Smn1tm1Jme/J | Repository- Live |
| Mice homozygous for this SMNF7 floxed allele are viable and fertile and do not display any gross physical or behavioral abnormalities. Mutant mice exhibit no transcript splicing defects. Cre-mediated recombination of the loxP-flanked sequences results in deletion of exon 7 of the targeted gene. As mutations of this exon are implicated in 95% of all human spinal muscular atrophy (SMA), these mice may be useful in studying SMA or other neuromuscular degenerative diseases.
When crossed to a strain expressing Cre recombinase in neurons (ssee Stock No. 005938, Stock No. 006297, and Stock No. 006663), this mutant mouse strain may be useful as a model of SMA. When crossed to a strain expressing Cre recombinase in striated muscle fibers (see Stock No. For more information please see the full descriiption on the strain data sheet | ||
| 006143 | FVB/N-Tg(Thy1-cre)1Vln/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is observed in nearly all neurons in cortex and hippocampus. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in postnatal, neuron-specific deletion of the flanked genome. These mice may be useful in studies of the nervous system, including Alzheimer's disease.
View cre expression characterization. | ||
| 004339 | STOCK Bdnftm3Jae/J | Repository- Live |
| These mice possess loxP sites on either side of exon 5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
When bred to a strain expressing Cre recombinase under the control of a tetracycline-responsive promoter element (see Stock No. 006224, 006234, 006244) and a strain expressing a tetracycline-controlled activator protein in brain tissues (see Stock No. 003763), this mutant mouse strain may be useful in studies of hippocampal-dependent learning and long-term potentiation. When bred to a strain expressing Cre recombinase in neuronal cells (see Stock No. 003966 for example), this
..... | ||
| 007674 | STOCK Esrrbtm1.1Nat/J | Repository- Live |
| Mice homozygous for this Nr3b2CKO allele possess loxP sites flanking exon 2 of the targeted gene and are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the exon containing the initiator methionine codon and encoding the N-terminal 132 amino acids (including part of the DNA-binding domain) deleted in the cre-expressing tissue(s). Of note, if the conditional Nr3b2CKO is deleted by Cre recombinase in the placenta and embryo, embryonic lethality will result (placental defect). If the conditional Nr3b2CKO is deleted by Cre recombinase only in the embryo, the resulting mice exhibit an inner ear defect (decreased endolymph production) resulting in deafness and defective balance. These Nr3b2CKO mutant mice may be useful in generating conditional mutations to study disorders of hearing and balance, inner ear development (such as endolymph-producing epithelia withi
..... For more information please see the full descriiption on the strain data sheet | ||
| 007913 | STOCK Gli1tm3(cre/ESR1)Alj/J | Repository- Live |
| Mice homozygous for this Gli1-CreERT2 targeted allele are viable and fertile (although homozygous males are reported to have breeding problems). Under control of the endogenous upstream promoter/enhancer elements, tamoxifen-inducible cre activity is observed in cells that have received positive Hedgehog/Sonic Hedgehog signaling. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Gli1-CreERT2 mice are
..... For more information please see the full descriiption on the strain data sheet | ||
| 005130 | STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J | Repository- Live |
| These mice contain an Enhanced Yellow Fluorescent Protein/Smoothened homolog (Drosophila) fusion gene inserted into the Gt(ROSA)26Sor locus. The mutant allele consists of a fusion product involving Enhanced Yellow Fluorescent Protein (EYFP) and the constitutively active W539L point mutation of the mouse smoothened homolog (Drosophila) gene (SmoM2). Expression of the Smo/EYFP fusion gene is blocked by a loxP-flanked STOP fragment placed between the Gt(ROSA)26Sor promoter and the Smo/EYFP sequence. When used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision results in the constitutive expression of mouse smoothened homolog (Drosophila) and unrestrained Hedgehog signaling in Cre-expressing tissues. Expression of the SmoM2 fusion protein can be monitored using EYFP-specific fluorescence protocols. Mice that are homozygous for the mutant allele are viable, fertile, normal in size and do not display any gross physical
..... For more information please see the full descriiption on the strain data sheet | ||
| 007576 | STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J | Repository- Live |
| Mice homozygous for this mT/mG mutation are viable and fertile. These mice possess loxP sites on either side of a membrane-targeted tdTomato (mT) cassette and express strong red fluorescence in all tissues and cell types examined. Tail or whole body epifluorescence is sufficient to identify mT/mG homozygotes. When bred to Cre recombinase expressing mice, the resulting offspring have the mT cassette deleted in the cre expressing tissue(s), allowing expression of the membrane-targeted EGFP (mG) cassette located just downstream. The donating investigator reports that the ACTB promoter allows stronger and persistent expression of the fluorescent proteins (especially in adult cells) compared to the endogenous Gt(ROSA) locus alone. This double-fluorescent system allows direct live visualization of both recombined and non-recombined cells at single cell resolution, offering an internal control for phenotypic analysis of Cre-induced mosaic mutants and providing a second marker fo
..... For more information please see the full descriiption on the strain data sheet | ||
| 007603 | STOCK Isl2tm1Arbr/J | Repository- Live |
| Mice homozygous for the Isl2DTA targeted mutation are viable and fertile. These mice have the diphtheria toxin (DTA) gene inserted into the Isl2 (insulin related protein 2 (islet 2)) locus. Expression of DTA in Isl2-expressing cells is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTA expression and subsequent cell ablation.
When bred to a strain expressing Cre recombinase in motor neurons (see Stock No. 006600 for example), this mutant mouse strain may be useful in neurodevelopmental studies. These Isl2DTA mutant mice are available on a STOCK genetic background (Stock No. 007603), as well as a C57BL/6J-backcrossed background (Stock No. 007942
..... | ||
| 007570 | STOCK Sim1tm1.2Az/J | Repository- Live |
| Mice homozygous for this "Sim1-floxed exon 1" (2-loxP) conditional allele are viable and fertile, with loxP sites flanking the translation start site and the first 17 amino acids of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for the basic domain of SIM1) deleted in the cre-expressing tissue(s). These "Sim1-floxed exon 1" (2-loxP) mice may be useful in generating conditional mutations for studying basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS) transcription factors, central nervous system development, early-onset/hyperphagic obesity, and regulation of appetite and energy balance. | ||
| 005438 | STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J | Repository- Live |
| While mice hemizygous for this Z/RED transgene are reported to be viable and fertile, it has been our experience at The Jackson Laboratory that hemizygous animals are often smaller than littermates and subject to postnatal mortality. Delayed weaning greatly enhances the survival. Although homozygous animals are born, animals have not survived past five weeks of age. These transgenic mice express beta-galactosidase under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with red fluorescent protein (DsRed*MST) expression in tissues expressing Cre recombinase. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells. | ||
| 006207 | STOCK Tg(Pcp2-cre)1Amc/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is observed in parasagittal domains of the cerebellum beginning at embryonic day 17 (E17). At E19, low level expression is observed in the most rostral lobe of cerebellum and expression broadens until all Purkinje cells express the transgene in adult mice. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome. These transgenic mice may be useful in studies utilizing “Cre-lox” technology, specifically regarding the nervous system, development and patterning of cerebellum, and cerebellar hypotrophy converse of Lhermitte-Duclos. | ||
| 005965 | STOCK Tg(Pomc1-cre)16Lowl/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is demonstrable in brain area neurons involved in the control of food intake (arcuate nucleus (hypothalamus) and solitary tract nucleus (hindbrain)). When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked genome in tissues that normally express Pomc1. The mice may be useful in studies of obesity, food intake, hunger, endocrine and exocrine function, and for tissue specific gene targeting. | ||
| 006395 | STOCK Tg(Sim1-cre)1Lowl/J | Repository- Live |
| Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Transgene expression is observed in all areas that endogenously express Sim1, including paraventricular hypothalamus and other parts of the brain. When these Sim1-Cre mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequences in Sim1-expressing tissues (including hypothalamus). As such, Sim1-Cre transgenic mice may be useful in studying body weight homeostasis, obesity, leptin metabolism, or as a reporter strain for Sim1-transcription factor activity. Of note, Sim1-Cre mice may also available on a C57BL/6J congenic background (see Stock No. 006451). | ||
| 006373 | 129-Braftm1Sva/J | Repository-Cryopreserved |
| Homozygous "floxed B-raf" (B-raff/f) mice are viable and fertile with normal B-raf protein expression. When bred to mice expressing Cre recombinase under the control of a promoter of interest, exon 12 of the targeted gene is deleted in the tissue of interest. These mutant mice may be useful in neurological studies such as Ras/Raf and MEK/ERK signaling, synaptic (neural) plasticity, learning and memory. For example, when crossed to a strain expressing Cre recombinase in nervous tissue (see Stock No. 003771), this mutant mouse strain may be useful in studies of neuron development. For example, when crossed to a strain expressing interferon inducible Cre recombinase (see Stock No. 003755), this mutant mouse strain may be useful in studies of extraembryonic mammmalian development. | ||
| 004318 | B6.129(FVB)-Gabra1tm1Geh/J | Repository-Cryopreserved |
| These mice possess loxP sites on either side of a Gabra1 exon encoding the second transmembrane domain. Mice that are homozygous for this floxed Gabra1 allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of Gabra1. In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available. Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations are available from this donating investigator (Dr. Gregg Homan
..... | ||
| 005247 | B6.129(SJL)-Nrp1tm2Ddg/J | Repository-Cryopreserved |
| These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. | ||
| 006891 | B6.129-Mgat1tm2Jxm/J | Repository-Cryopreserved |
| Mice homozygous for this floxed targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to tissue-specific cre transgenic strains, the loxP-flanked exon is deleted by cre expression. | ||
| 005697 | B6.129-Otx1tm4(cre)Asim/J | Repository-Cryopreserved |
| This strain expresses Cre recombinase from the targeted locus. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes have a perinatal lethal phenotype. Expression of the Cre recombinase gene (mRNA) under the control of the endogenous gene promoter, is detected in the lateral midbrain of embryonic day 10.5 aged embryos and in the presumptive alar-basal plate boundary of embryonic day 12.5 aged embryos, closely mimicking the endogenous gene expression pattern. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination is first detected at embryonic day 8.7. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study developmentally critical gene function in the brain. | ||
| 005357 | B6.129-Sema3ftm1.1Ddg/J | Repository-Cryopreserved |
| These mice possess loxP sites 4kb upstream of exon 1 and 1 kb downstream of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to a strain expressing Cre recombinase in neuronal cells (see Stock No. 003966 for example), this mutant mouse strain may be useful in neurobiology research. | ||
| 008133 | B6.129-Sncbtm1Sud/J | Repository-Cryopreserved |
| These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, normal in size and do not display any gross physical or behavioral abnormalities, but breed very poorly. Protein levels are normal. When bred to a strain expressing Cre recombinase, this mutant mouse strain may be useful in studies of presynaptic proteins and synaptic vesicles. | ||
| 003966 | B6.Cg-Tg(Syn1-cre)671Jxm/J | Repository-Cryopreserved |
| These transgenic mice express Cre recombinase under the direction of a synapsin promoter. Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity is detected in neuronal cells by embryonic day 12.5. | ||
| 005630 | B6.Cg-Tg(Thy1-EYFP)15Jrs/J | Repository-Cryopreserved |
| These transgenic mice conditionally express Enhanced Yellow Fluorescent Protein (EYFP) under the direction of the mouse thymus cell antigen 1, theta, promoter. Expression of the EYFP gene is blocked by a loxP-flanked STOP fragment placed between the promoter and EYFP gene. Cre-mediated excision of the STOP cassette results in expression of EYFP in motor neurons. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. For example, when crossed to a strain with widespread expression of Cre recombinase (see Stock No. 003376), this mutant mouse strain may be useful in studies of synaptic function. | ||
| 002982 | B6.Cg-Tg(xstpx-lacZ)32And/J | Repository-Cryopreserved |
| This mutant, when crossed with a cre transgenic, will express lacZ in cells where cre is expressed to remove the STOP of translation section which lies between the 2 loxP sites. LacZ expression is restricted to neural and skeletal muscle tissue and heart by the chicken beta-actin promoter that is driving the reporter. | ||
| 006382 | B6;129-Casktm1Sud/J | Repository-Cryopreserved |
| Homozygous floxed mice are viable and fertile, but females do not thrive. The body size of mutants is significantly smaller than littermate controls and they exhibit a slightly increased mortality. Knock-in mice are hypomorphs and protein is expressed at less than 30% of normal levels. Crossing of the floxed mutants with mice expressing cre recombinase in the male germline excises the floxed exon and a neomycin resistance gene cassette to create a complete knockout of the gene. Knockout homozygotes die within a few hours of birth. They exhibit a partially penetrant cleft palate syndrome and increased apoptosis in the thalamus, but display no other major developmental changes or deficits in basic electrical properties of their neurons.
When bred to a strain expressing Cre recombinase in the male germline (see Stock No. 003328 or 007252 for example), this mutant mouse str
..... | ||
| 007855 | B6;129-Rims2tm1.2Schc/J | Repository-Cryopreserved |
| Mice homozygous for this floxed targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exon 5 is deleted by cre expression to produce a "RIM2alpha" isoform null allele. This strain may be helpful in the analysis of the function of alpha-RIMs in specific brain regions. | ||
| 006575 | C57BL/6-Camk2atm1Vyb/J | Repository-Cryopreserved |
| Mice homozygous for this "falpha-CaMKII" allele are viable and fertile with no reported neurological abnormalities. These mutant mice have loxP sites flanking exon 2 of the endogenous gene. When bred to Cre-recombinase expressing mice, exon 2 is deleted in the resulting offspring dependent on the tissue specificity of the promoter of the cre transgene. For example, when crossed with transgenic mice expressing Cre-recombinase in hippocampal CA3 pyramidal cells (see Stock No. 006474), the resulting offspring show altered neurotransmitter release. These "floxed" mice may be useful for neurological studies such as calcium/calmodulin-dependent protein kinase activity. | ||
| 006581 | C57BL/6-Ppp3r1tm1Stl/J | Repository-Cryopreserved |
| Mice homozygous for this "fCNB1" mutant allele are viable and fertile with no reported neurological abnormalities. These mutant mice have loxP sites flanking exons 2-4 of the endogenous gene. When bred to transgenic mice expressing Cre recombinase, exons 2-4 are deleted in the resulting offspring in only those tissues expressing cre. For example, when crossed with transgenic mice expressing Cre recombinase specifically in the forebrain (similar to Stock No. 005359), the resulting offspring show abnormalities that are strikingly similar to those described for schizophrenia. These "floxed" mice may be useful in studies of calcineurin function in T cells (via NFAT transcription of cytokine genes) and in the central nervous system in, for example, neurite extension, synaptic plasticity, learning and memory, and schizophrenia pathogenesis. | ||
| 006662 | C57BL/6-Tg(ACTB-MAP2K1*K97M)1Stl/J | Repository-Cryopreserved |
| Hemizygous mice are viable and fertile. These "dnMEK1" mice express a dominant-negative mutant (K97M) form of human MEK1 (synonym: MAP2K1) following Cre-mediated removal of the upstream "Lox-STOP-Lox" cassette; when transgenic mice are bred to a cre-expressing strain, the "floxed stop" cassete is excised in the resulting offspring, and mutant MEK1 expression is observed in the cre-expressing tissue(s). In the absence of Cre recombinase, transgene expression is not detectable in the brains of these "floxed" mice Because the MEK1 mutation abolishes the protein's kinase activity but preserves its ability to interact with ERK1 and ERK2, these transgenic mice may be useful in studying MEK-dependent activation and regulation of ERK, the ERK-MAPK signaling pathway, and neurological studies involving synaptic plasticity and memory. When bred to a strain expressing Cre recombinase in the CA1 pyramidal cell layer of the hippocampus (see Stock No. For more information please see the full descriiption on the strain data sheet | ||
| 002858 | STOCK Tg(Nes-cre)1Wme/J | Repository-Cryopreserved |
| These transgenic mice carry the Cre recombinase gene under the control of the rat nestin promoter. When crossed with a strain carrying a gene flanked by loxP sites, partial deletion of the loxP-flanked allele occurs before embryonic day 10.5 and is detectable in all adult organs examined including germ line cells. Because of the partial deletion, these balancer1 cre transgenic mice, as well as the balancer2 cre transgenic mice (Stock No. 002859), may be used to generate mosaic mice consisting of cells that are either wildtype or mutant for the gene of interest. The proportion of cells that carry the mutant allele varies between tissues and between individual mice. In cases where deletion of a particular gene is lethal, the generation of mosaic mice using these strains can bypass lethality in some mosaic individuals. The balancer2 line induces more variable deletion of loxP-flanked genes than the
..... For more information please see the full descriiption on the strain data sheet | ||
| 002859 | STOCK Tg(Nes-cre)2Wme/J | Repository-Cryopreserved |
| These transgenic mice carry the Cre recombinase gene under the control of the rat nestin promoter. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing cre, generating a proportion of mosaic animals. All adult organs, including germ-line cells, may be affected. The balancer2 line of transgenic mice show greater variability and instability in their generation of mosaics than the balancer1 line (Stock No. 002858). This variability of the balancer2 line can be of particular benefit if the gene of interest is vital and mosaic individuals are used to bypass lethality. | ||
(71 stocks) Back to Top
How to Register Interest
Please indicate your interest in purchasing any of the strains listed below when they become available for distribution by checking the box next to the strain(s) of interest and then selecting the "Continue" button which leads to an Interest Form.View a Data sheet for New Strains Under Development
Select the strain name to link to the strain data sheet.
New Strains Under DevelopmentThe Jackson Laboratory serves as a worldwide distributor and national repository for common and rare strains of inbred mice and mice carrying spontaneous mutations or induced mutations (i.e., transgenic, targeted/"knockout", or chemically induced mutations). At any one time, we have over 100 strains at various stages of development and colony expansion. Strains "Under Development" fall into two categories depending on the anticipated demand from the scientific community.
- Receive periodic updates on the status of the colony UNDER DEVELOPMENT
- Obtain advance notification of strain availability and opportunity to order prior to the strain being published as available
- Provide input affecting speed and quantity of availability
It is VERY IMPORTANT that you register interest in strains Under Development. The anticipated demand for a strain enables us to determine effectively the distribution plan for each strain Under Development. Registering interest also provides benefits to you (including advance notification of pending availability). Whether a strain is made available from a live colony OR from our cryopreservation repository, you may want to consider the option of Dedicated Supply. To learn more about Dedicated Supply, go to Services.
- Strains that will be made available from a live distribution colony at The Jackson Laboratory.
These strains are designated as: "Under Development for Distribution Colony"- Strains that will be made available through the Cryopreservation Repository.
These strains are designated as: "Under Development for Cryopreservation Repository"
Send questions to our Technical Support team using the Express Technical Support Form.
(3.2)