Search Criteria: Research Area is "Neurobiology Research: Tet Expression System (tTA/rtTA Expressing Strains)"

New Strains Under Development

JAX® Mice Strains

Stock
Number
Strain Name
 
Strain Description
Standard Supply
006965 B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
Repository- Live
Homozygotes are viable, fertile, normal in size and do not display any behavioral abnormalities. These targeted mutant mice have widespread expression of an optimized form of reverse tetracycline-controlled transactivator (rtTA-M2) protein. This R26-M2rtTA strain may be useful for doxycycline-inducible studies which utilize rtTA/tet-O (tet-on/TRE) models.
007004 B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ
Repository- Live
Transgenic mice expressing the tetracycline-controlled transactivator protein (tTA) under regulatory control of the forebrain-specific calcium-calmodulin-dependent kinase II (Camk2a) promoter are viable and fertile. When hemizygotes are mated to a second strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene can be blocked by administration of the tetracycline analog, doxycycline (dox). These mice are a "Tet-Off" tool that allow the inducible expression of genes in forebrain neurons, and may be useful in studying brain disorders such as Alzheimer's disease (when used in conjunction with Stock No. 005706, Stock No. 007049, Stock No. 007051, Stock No. 007052), Parkinson's .....
For more information please see the full descriiption on the strain data sheet
005964 B6.Cg-Tg(GFAP-tTA)110Pop/J
Repository- Live
Mice hemizygous for the transgene are viable fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice express a tetracycline-controlled transactivator protein (tTA) driven by the human glial fibrillary acidic protein (GFAP) promoter. When these mice are mated to a second transgenic strain that carries a target gene under the regulation of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in astrocytes in the bitransgenic offspring can be induced by withdrawal of the tetracycline analog, doxycycline. Doxycycline may be administered in the animals' water supply. This strain represents an effective tool for generating bitrangenic animals that may be useful to study inducible gene expression in the astrocytes of the central nervous system.
003010 B6;CBA-Tg(Camk2a-tTA)1Mmay/J
Repository- Live
Transgenic mice expressing the tetracycline-controlled transactivator protein (tTA) under regulatory control of the forebrain-specific calcium-calmodulin-dependent kinase II (Camk2a) promoter are viable, fertile, and display no overt phenotypic defects. Transgene expression can be blocked by the administration of the tetracycline analog doxycycline (dox) to the mice. Mating these transgenic mice to a second transgenic strain carrying a gene of interest coupled to a tetracycline-responsive promoter element (TRE; tetO) allows dox-inducible expression of the target gene specifically in forebrain neurons, and may be useful in studying brain disorders such as Alzheimer's disease (when used in conjunction with Stock No. 005706, Stock No. 007049, Stock No. 007051, Stock No. 00 .....
For more information please see the full descriiption on the strain data sheet
003767 B6.Cg-Tg(Eno2tTA)5021Nes/J
Repository-Cryopreserved
These transgenic mice express the tetracycline-controlled activator protein (tTA) under the control of a rat neuron-specific enolase (Eno2) promoter. Although the Eno2 promoter has been shown to direct high levels of expression in brain tissue in general, this strain selectively expresses tTA at high levels in the striatum and cerebellum. When B6.Cg-Tg(Eno2tTA)5021Nes/J transgenic mice are mated to a second transgenic strain carrying a gene of interest under the regulation of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in the bitransgenic offspring may be induced in the tTA-expressing tissues by the withdrawal of the tetracycline analog, doxycycline (dox). For example, when these transgenic mice are bred with Stock No. 003762, a transgenic strain that expresses a Fosb variant under the direction of a TRE, the resulting bitransgenic offspring can be induced to express h .....
For more information please see the full descriiption on the strain data sheet
003763 B6.Cg-Tg(Eno2tTA)5030Nes/J
Repository-Cryopreserved
These transgenic mice express the tetracycline-controlled activator protein (tTA) under the control of a rat neuron-specific enolase (Eno2) promoter. Although the Eno2 promoter has been shown to direct high levels of expression in brain tissue in general, this strain selectively expresses tTA at high levels in the striatum and to a lesser extent in the cerebral cortex and hippocampus. When these transgenic mice are mated to a second transgenic strain carrying a gene of interest under the regulation of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in the bitransgenic offspring may be induced in the tTA-expressing tissues by the withdrawl of the tetracycline analog, doxycycline (dox). For example, when these transgenic mice are bred with Stock No. 003762, a transgenic strain that expresses a Fosb variant under the direction of a TRE, the resulting bitransgenic offspring ca .....
For more information please see the full descriiption on the strain data sheet
008284 B6.Cg-Tg(Scg2-tTA)1Jt/J
Repository-Cryopreserved
Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These mice express tetracycline-controlled transactivator protein (tTA) under the control of a mouse secretogranin II promoter. When these mice are mated to a second transgenic strain that carries a gene of interest driven by a tetracycline-responsive promoter element (TRE; tetO), expression of that gene can be conditionally regulated by the presence or absence of doxycycline in the drinking water. Expression in the suprachiasmatic nucleus and brain of bitransgenic animals can be reversibly inhibited by the presence of doxycycline or induced by withdrawal of the tetracycline analog.
004127 FVB-Tg(Nes-rtTA)306Rvs/J
Repository-Cryopreserved
Mice that are hemizygous for this transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat nestin intron II enhancer/promoter. According to the donating investigator, expression occurs in the neuroepithelium of the developing nervous system (embryonic days 10-17), in some neuron subsets of the adult mouse (e.g. cerebellum, hippocampal dentate gyrus, subventricular zone, spinal cord) and in adult testes. The rtTA gene is cotranscribed with the lacZ reporter gene Bgeo, which allows verification of the site of rtTA expression. When Tg(Nes-rtTA) hemizygous mice are mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycli .....
For more information please see the full descriiption on the strain data sheet
005625 FVB-Tg(Pcp2-tTA)3Horr/J
Repository-Cryopreserved
Mice that are homozygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cerebellar Purkinje cell-specific tTA expression has been confirmed using PCR. Constitutive expression of tTA in Purkinje cells makes this strain suitable for creating bitransgenic mice that can, by withdrawing tetracycline (or its derivative doxycycline), inducibly express a gene of interest in Purkinje cells when the gene of interest is under the direction of a tetracycline-responsive element (TRE; tetO).

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New Strains Under Development

(See informational text following listing of strains)
How to Register Interest
Please indicate your interest in purchasing any of the strains listed below when they become available for distribution by checking the box next to the strain(s) of interest and then selecting the "Continue" button which leads to an Interest Form.

View a Data sheet for New Strains Under Development
Select the strain name to link to the strain data sheet.

Stock
Number
Strain Name
 
Strain Description
Standard Supply
008600B6.129P2-Gt(ROSA)26Sortm1(tTA)Roos/J
Under Development for Production
Mice heterozygous for the ROSA:LNL:tTA conditional mutation of the Gt(ROSA)26Sor locus are viable and fertile, with a loxP-flanked STOP cassette preventing transcription of a downstream optimized/modified tetracycline-controlled transactivator protein ("mtTA"). Applying both Cre-lox and Tet-Off technologies, these ROSA:LNL:tTA mutant mice may be useful to generate compound mutant mice in which expression of a tetracycline-responsive promoter element (TRE; also called tet-operator or tetO)-driven gene of interest can be both directed to the cell types defined by the chosen Cre recombinase expression, as well as turned off by the addition of tetracycline (or its analog doxycycline (dox)).
008344B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J
Under Development for Production
The TetTag mouse is a bi-transgenic mutant that has tetracycline (or tetracycline analog) inducible expression of beta-galactosidase in activated neurons. Two independently generated transgenic strains were crossed to produce this bi-transgenic TetTag strain. In the first transgenic construct, the tetracycline-controlled transactivator (rTA) protein and a two hour half-life Green Fluorescent Protein (shEGFP) are expressed under the direction of the fos, FBJ osteosarcoma oncogene, minimal promoter. The second transgenic construct expresses a nuclear-localizing beta-galactosidase gene and the doxycycline insensitive tetracycline regulated transactivator (containing point mutation, H100Y), under the control of the tetO, tetracycline-responsive regulatory element. In the absence of tetracycline or a tetracycline analog (such as doxycycline), selective expression of beta-galactosidase is observed in activated neurons. Doxycycline administration prevents expression beta-galactosidase .....
For more information please see the full description on the strain data sheet

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New Strains Under Development The Jackson Laboratory serves as a worldwide distributor and national repository for common and rare strains of inbred mice and mice carrying spontaneous mutations or induced mutations (i.e., transgenic, targeted/"knockout", or chemically induced mutations). At any one time, we have over 100 strains at various stages of development and colony expansion. Strains "Under Development" fall into two categories depending on the anticipated demand from the scientific community.
  1. Strains that will be made available from a live distribution colony at The Jackson Laboratory.
    These strains are designated as: "Under Development for Distribution Colony"
  2. Strains that will be made available through the Cryopreservation Repository.
    These strains are designated as: "Under Development for Cryopreservation Repository"
It is VERY IMPORTANT that you register interest in strains Under Development. The anticipated demand for a strain enables us to determine effectively the distribution plan for each strain Under Development. Registering interest also provides benefits to you (including advance notification of pending availability). Whether a strain is made available from a live colony OR from our cryopreservation repository, you may want to consider the option of Dedicated Supply. To learn more about Dedicated Supply, go to Services.

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