Search Criteria: Research Area is "Cardiovascular Research: Hypertension (normotensive)"
| Stock Number |
Strain Name Strain Description |
Standard Supply |
| 008082 | B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J | Repository- Live |
| Hemizygous tTA+/RVCH+ (or SM22a-tTA/TRE-RVCH-HA) mice are viable and fertile, harboring two transgenes using the Tet-Off system. The SM22a-tTA transgene has the 3 kb SM22alpha promoter directing expression of the tetracycline transactivator gene (tTA) to vascular smooth muscle cells (SMCs). The TRE-RVCH-HA transgene has the tetracycline-responsive element (TRE; also called tet-operator or tetO) controlling expression of a rat vascular chymase-hemagglutinin tag (RVCH-HA) fusion gene. In the absence of tetracycline (or its analog doxycycline (dox)), the SM22alpha promoter limits RVCH-HA fusion protein expression to vascular SMCs. This RVCH overexpression results in hypertension. Because this binary transgenic system also allows for a second level of control, i.e. addition of dox, expression of the RVCH-HA fusion protein can be completely abolished; reversing the hypertension phenotype. These SM22a-tTA/TRE-RVCH-HA bi-transgenic mice allow targeted overexpression or Tet-Off conditio
..... For more information please see the full descriiption on the strain data sheet | ||
| 002750 | C57BL/6-Tg(hREN)9Sig/J | Repository-Cryopreserved |
| Mice carrying the human renin transgene have high levels of expression in the kidney, adrenal gland, ovary, testis, lung and adipose. No expression is evident in the liver or brain. Active renin is released into the plasma of the transgenic mice. These mice are normotensive but a chronically hypertensive mouse can be created by breeding this strain with a strain carrying the human angiotensinogen transgene (C57BL/6-TgN(hAGT)2041Sig; 002749). The mean arterial pressure of the double transgenic is ~155 mmHg (controls ~111 mmHg). Both systolic and diastolic blood pressures are elevated. | ||
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