Research Tools: FLP-FRT System

• FLP Recombinase Expression: Germline/Embryonic Expression
• FRT-flanked Sequences
• FRT-flanked Sequences: Test/Reporter

FLP Recombinase Expression: Germline/Embryonic Expression

Stock No	Strain Name	Promoter (Species)	Standard Supply
	Site of Expression
003800	B6;SJL-Tg(ACTFLPe)9205Dym/J	ACTB, actin, beta (human)	Repository- Live
	expression of the enhanced FLP1 recombinase variant (FLPe) is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5
005703	B6.Cg-Tg(ACTFLPe)9205Dym/J	ACTB, actin, beta (human)	Repository- Live
	expression of the enhanced FLP1 recombinase variant (FLPe) is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5
003946	129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J	Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano	Repository- Live
	constitutive expression of FLP1 recombinase from preimplantation onward and in most tissue types (including cells of the developing germ line)

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FRT-flanked Sequences

Stock No	Strain Name	Flanked Gene (Species)	Promoter (Species)	Standard Supply
008242	C57BL/6-Gt(ROSA)26Sortm1(Ikbkb)Rsky/J		Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano	Under Development for Production
007901	B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J	CFP, Cyan Fluorescent Protein (jellyfish) YFP, Yellow Fluorescent Protein (jellyfish) RFP, Red Fluorescent Protein (jelly fish)	Thy1, thymus cell antigen 1, theta (mouse)	Repository- Live
007910	B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J	CFP, Cyan Fluorescent Protein (jellyfish) YFP, Yellow Fluorescent Protein (jellyfish) RFP, Red Fluorescent Protein (jelly fish)	Thy1, thymus cell antigen 1, theta (mouse)	Repository- Live
007911	B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J	CFP, Cyan Fluorescent Protein (jellyfish) YFP, Yellow Fluorescent Protein (jellyfish) RFP, Red Fluorescent Protein (jelly fish) OFP, Orange Fluorescent Protein (jelly fish)	Thy1, thymus cell antigen 1, theta (mouse)	Repository- Live
007921	B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J	CFP, Cyan Fluorescent Protein (jellyfish) GFP, Green Fluorescent Protein (jellyfish) YFP, Yellow Fluorescent Protein (jellyfish) RFP, Red Fluorescent Protein (jelly fish)	Thy1, thymus cell antigen 1, theta (mouse)	Repository- Live

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FRT-flanked Sequences: Test/Reporter

Stock No	Strain Name	Promoter (Species)	Standard Supply
	Site of Expression
004598	B6;129S4-Gt(ROSA)26Sortm2Dym/J	Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano	Repository-Cryopreserved
	when crossed to a FLP recombinase-expressing strain, expression of human placental alkaline phosphatase (ALPP) is observed in the FLP-expressing tissues
007903	B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J	Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano	Under Development for Production
007905	B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J	Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano	Under Development for Production
007906	B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J	Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano	Under Development for Production
007909	B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J	Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano	Under Development for Production
007920	B6.Cg-Gt(ROSA)26Sortm2(CAG-EYFP)Hze/J	Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano	Under Development for Production

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