September 25, 2012

Available Soon: New Mouse Models for Immunology and Inflammation Research

The following three mouse models for immunology and  inflammation research will be available soon:

B6N.129P2-Il27ratm1Mak/J (018078)

  • Harbors a knockout mutation in the interleukin 27 receptor, alpha (Il27ra or WSX-1) gene, which encodes a class I cytokine receptor that induces T helper 1 (Th1) cell differentiation and regulates cell-mediated immunity
  • Homozygotes exhibit impaired IFNG production during early stages of Leishmania major infection
  • In contrast, Mycobacterium tuberculosis infection enhances CD4+ T cell activation and IFNG production in homozygotes
  • In a Con A-induced model of hepatitis, natural killer T cells produce high levels of IFNG, interleukin 4 (IL4), IL1, IL6, and tumor necrosis factor alpha (TNFA)
  • May be used to study the regulation of Th1/Th2-induced cytokine production during infection and inflammation
  • Now accepting orders; estimated distribution date: October 15

B6.129S4-Ifngtm3.1Lky/J (017581)

  • Harbors the "interferon-gamma reporter with endogenous polyA transcript" (GREAT) allele, which has an IRES-eYFP reporter cassette targeted to the endogenous locus between the translational stop codon and the 3' UTR/polyA tail of the interferon gamma (Ifng) gene
  • GREAT expression is controlled by the endogenous IFNG promoter/enhancer regions
  • Enhanced yellow fluorescent protein (eYFP) is detectable by FACS and microscopy in IFNG-expressing cells, including innate immune cells (natural killer cells, natural killer T cells), and adaptive immune cells (CD4+ and CD8+ T cells)
  • Overcomes limitations of the IFNG-IRES-YFP-BGHpolyA knockin (YETI) model, which aberrantly expresses IFNG-IRES-YFP, resulting in high IFNG expression and premature death
  • Now accepting orders; estimated distribution date: November 19

NOD.Cg-Prkdcscid Il2rgtm1Wjl H2-Ab1tm1Gru Tg(HLA-DRB1)31Dmz/SzJ (017637)

  • Severely immunodeficient strain that does not express the murine Prkdc gene, the X-linked Il2rg gene, and MHC class II, but expresses the human leukocyte antigen DR4 transgene
  • Unlike NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG, 000557), expresses HLA-DR4, resulting in allo-graft-versus-host disease (GVHD) following engraftment of human DR4-negative CD4+ T cells
  • May be used to target human CD4+ T cells in transplantation studies in the absence of xenograft-GVHD
  • Now accepting orders; estimated distribution date: September 24

Visit our immunology website to see our selection of excellent mouse models, services, web resources, and guides for immunology and inflammation research.