|August 29, 2012|
Less Leaky Lung-Specific rtTA Strains Available
We are distributing two new and improved “Tet-On” tool strains that can be used to induce/control the expression of genes in the developing and adult lung:
- STOCK Tg(Scgb1a1-rtTA)2Jaw/J (016145) (see Perl et al. 2009)
- STOCK Tg(SFTPC-rtTA)2Jaw/J (016146) (see Chen et al. 2011)
Both strains express the reverse tetracycline-controlled transactivator (rtTA) protein following induction with doxycycline (DOX). They express rtTA only in cells that use specific promoter sequences.
In strain 016145, rtTA is regulated by the rat secretoglobin (Scgb1a1) gene promoter. DOX induces rtTA expression in the proximal conducting airway epithelia and type II alveolar cells of adults and embryos as early as embryonic day 14.
In strain 016146, rtTA is regulated by the human surfactant protein C (SFTPC) gene promoter. DOX induces rtTA expression in distal lung structures, including non-ciliated bronchiolar epithelial cells (but rarely in alveolar type II cells), of adults and embryos as early as embryonic day 12.5.
When either strain is mated to a strain harboring a transgene controlled by a tetracycline-responsive promoter element (tetO), the transgene’s expression in the offspring may be induced with DOX. For example, mating either strain with FVB-Tg(tetO/CMV-KRAS*G12C)9.1Msmi/J (006439) produces offspring that can be used as inducible models of lung cancer.
Unlike in similar strains – B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J (006232), FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J (006222), C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J (006242) B6.Cg-Tg(SFTPC-rtTA)5Jaw/J (006235), FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J (006225), and C.Cg-Tg(SFTPC-rtTA)5Jaw/J (006245) – the expression of rtTA in these generation strains is not leaky (not expressed in the absence of DOX).
If you do not have the space or capabilities to produce double transgenic mice, we can produce them for you – and provide you with controls. Call us at 1-800-422-6423 (US, Canada or Puerto Rico) or 1-207-288-5845 (from any location).