Stock Number:012345
Strain Name:STOCK Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo/J

Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo
Tg(tetO-tdTomato,-Syp/EGFP*)
Standard PCR
2.0
31-March -2011 31-March -2011
Notes: This assay will NOT distinguish hemizygous from homozygous transgenic animals.
Expected Results:

Transgene = 420 bp
Internal control = 200 bp

Gel Image 
Image Image file
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Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
11115 - CTT CAA GTC CGC CAT GCC CGA - Transgene Forward
11116 - TCC AGC AGG ACC ATG TGA TCG C - Transgene Reverse
oIMR8744 - CAA ATG TTG CTT GTC TGG TG - Wild type Forward
oIMR8745 - GTC AGT CGA GTG CAC AGT TT - Wild type Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration
Total Volume (µl)
2.5 mM dNTP 0.96 0.20 mM 0.96
DNA 2.00 - 2.00
ddH2O 3.32 - 3.32
5 mM DNA Loading Dye 1.66 0.69 mM 1.66
20 uM oIMR8745 0.80 1.33 uM 0.80
20 uM oIMR8744 0.80 1.33 uM 0.80
20 uM 11115 0.60 1.00 uM 0.60
10 X KAPA Rxn Buffer 1.20 1.00 X 1.20
5 U/ul KAPATaq DNA Polymerase 0.06 0.03 U/ul 0.06
20 uM 11116 0.60 1.00 uM 0.60
Cycling 
Step # Temp °C Time Note
1 94 3 min -
2 94 30 sec -
3 64 30 sec -
4 72 30 sec -
5 - - Go to step 2-4, 35 times
6 72 2 min -
7 10 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
012345 STOCK Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo/J
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.