Stock Number:006847
Strain Name:B6;129P2-Mecp2tm1Bird/J
Mecp2tm1Bird
Mecp2tm1Bird
Standard PCR
5.0
30-September-2011 30-September-2011
Notes: The Mecp2 gene is X-LINKED. Males with the targeted allele are hemizygous mutants
Expected Results:

Mutant = 480 bp
Heterozygote = 480 bp and 416 bp
Wild type = 416 bp

**To genotype for null allele (post cre excision) use oIMR6835, TCC ACC TAG CCT GCC TGT ACT TTG with oIMR6833 to amplify a 420bp fragment from the null allele.**

 
Gel Image 
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Gel Information: Separated by gel electrophoresis on a 3% agarose gel.
Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
oIMR6833 - TGG TAA AGA CCC ATG TGA CCC AAG - Forward
oIMR6834 - GGC TTG CCA CAT GAC AAG AC - Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
ddH2O 6.58 - 6.58
10 X KAPA Rxn Buffer 1.20 1.00 X 1.20
2.5 mM dNTP 0.96 0.20 mM 0.96
20 uM oIMR6833 0.60 1.00 uM 0.60
20 uM oIMR6834 0.60 1.00 uM 0.60
5 U/ul KAPATaq DNA Polymerase 0.06 0.03 U/ul 0.06
DNA 2.00 0.00 2.00
Cycling 
Step # Temp °C Time Note
1 94 3 min -
2 94 30 sec -
3 70 1 min -
4 72 30 sec repeat steps 2-4 for 35 cycles
5 72 2 min -
6 10 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
006847 B6;129P2-Mecp2tm1Bird/J
007177 B6.129P2-Mecp2tm1Bird/J
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.