Stock Number:007856
Strain Name:B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J

Tg(Thy1-Syt1/ECFP)1Sud
Tg(Thy1-Syt1/ECFP)1Sud
Standard PCR
1.1
08-July -2008 27-April -2010
Expected Results: Transgene = 700 bp
Gel Information: Separated by gel electrophoresis on a 1.5% agarose gel.
Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
oIMR7604 - TTG AGA CTA AAG TCC ACC GG - Forward
oIMR7605 - CTT ATC GAT ACG CGT GGT AC - Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration
Total Volume (µl)
ddH2O 3.96 - 3.96
10 X AB PCR BufferII 0.80 0.67 X 0.80
25 mM MgCl2 0.96 2.00 mM 0.96
2.5 mM dNTP 0.96 0.20 mM 0.96
20 uM oIMR7604 0.80 1.33 uM 0.80
20 uM oIMR7605 0.80 1.33 uM 0.80
5 mM DNA Loading Dye 1.66 0.69 mM 1.66
5 U/ul Taq DNA Polymerase 0.06 0.03 U/ul 0.06
DNA 2.00 - 2.00
Cycling 
Step # Temp °C Time Note
1 94 3 min -
2 94 30 sec -
3 60 1 min -
4 72 1 min repeat steps 2-4 for 35 cycles
5 72 2 min -
6 10 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
007856 B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.