Stock Number: 008737 Strain Name: FVB-Tg(Ckm-Ppargc1b)T37Brsp/J

Allele: Tg(Ckm-Ppargc1b)T37Brsp Protocol Name: Tg(Ckm-Ppargc1b Method: Standard PCR Version: 1.0 Created: 11-August -2009 Updated: 07-May -2010

Gel Image

no data found

Gel Information: 1.5% gel

Protocol Primers

Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type 9151 - CCC AGC GTC TGA CGT GGA CGA GC - Transgene Forward 9152 - CCT TCA GAG CGT CAG AGC TTG CTG - Transgene Reverse oIMR7338 - CTA GGC CAC AGA ATT GAA AGA TCT - Internal Positive Control Forward oIMR7339 - GTA GGT GGA AAT TCT AGC ATC ATC C - Internal Positive Control Reverse

Number of Reactions

Reaction/Components  Reaction Component Volume (µl) Final Concentration Total Volume (µl) 5 mM DNA Loading Dye 1.66 0.69 mM 1.66 DNA 2.00 - 2.00 5 U/ul Taq DNA Polymerase 0.06 0.03 U/ul 0.06 ddH2O 2.76 - 2.76 25 mM MgCl2 0.96 2.00 mM 0.96 2.5 mM dNTP 0.96 0.20 mM 0.96 20 uM 9151 0.60 1.00 uM 0.60 20 uM 9152 0.60 1.00 uM 0.60 20 uM oIMR7338 0.60 1.00 uM 0.60 20 uM oIMR7339 0.60 1.00 uM 0.60 10 X AB PCR BufferII 1.20 1.00 X 1.20

Cycling  Step # Temp °C Time Note 1 94 3 min - 2 94 30 sec - 3 59 1 min - 4 72 1 min - 5 - - Go to step 2-4, 35 times 6 72 2 min - 7 10 - hold

This genotyping protocol is used for the following strains:   Stock Number Strain Name 008737 FVB-Tg(Ckm-Ppargc1b)T37Brsp/J Genotyping resources and troubleshooting This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.