Stock Number:016833
Strain Name:FVB(Cg)-Tg(Alb1-cre)1Dlr/J
Tg(Alb1-cre)1Dlr
Tg(Alb1-cre)1Dlr
Standard PCR
1.0
15-June -2011 15-September-2011
Notes: This assay will NOT distinguish hemizygous from homozygous transgenic animals.
Expected Results:

Transgene = 500 bp

Internal control = 324 bp
Gel Image 
Image Image file
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Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
12591 - CCA GGC TAA GTG CCT TCT CTA CA - Transgene Forward
12592 - AAT GCT TCT GTC CGT TTG CCG GT - Transgene Reverse
oIMR7338 - CTA GGC CAC AGA ATT GAA AGA TCT - Internal Positive Control Forward
oIMR7339 - GTA GGT GGA AAT TCT AGC ATC ATC C - Internal Positive Control Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
2.5 mM dNTP 0.96 0.20 mM 0.96
DNA 2.00 - 2.00
ddH2O 4.02 - 4.02
5 mM DNA Loading Dye 1.66 0.69 mM 1.66
20 uM 12591 0.60 1.00 uM 0.60
20 uM 12592 0.60 1.00 uM 0.60
10 X KAPA Rxn Buffer 1.20 1.00 X 1.20
20 uM oIMR7338 0.45 0.75 uM 0.45
20 uM oIMR7339 0.45 0.75 uM 0.45
5 U/ul KAPATaq DNA Polymerase 0.06 0.03 U/ul 0.06
Cycling 
Step # Temp °C Time Note
1 94 3 min -
2 94 30sec -
3 62 30sec -
4 72 30sec -
5 - - Go to step 2-4, 35 times
6 72 2 min -
7 10 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
016832 B6.FVB(129)-Tg(Alb1-cre)1Dlr/J
016833 FVB(Cg)-Tg(Alb1-cre)1Dlr/J
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.