Stock Number:000632
Strain Name:B6.Cg-Lepob/J
Lepob
Lep ob
End Point Analysis
2.0
26-January -2012 30-May -2012
Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
13373 GTG CTG CAG ATA GCC AAT GAC -
13374 GAG AAG GCC AGC AGA TGG A -
13375 JOE NHS Ester TGG AGA ATC TCC GAG ACC Black Hole Quencher 1 -
13667 6-FAM CTG GAG AAT CTC TGA GAC C

Iowa Black

 -
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
2 X Kapa Probe Fast QPCR 2.50 1.00 X 2.50
100 uM 13373 0.02 0.00 uM 0.02
DNA 2.37 0.00 2.37
ddH2O 0.07 0.00 0.07
100 uM 13374 0.02 0.00 uM 0.02
100 uM 13667 0.01 0.00 uM 0.01
100 uM 13375 0.01 0.00 uM 0.01
Cycling 
Step # Temp °C Time Note
1 95 10 min -
2 92 15 sec -
3 60 1 min repeat steps 2-3 for 40 cycles
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
000632 B6.Cg-Lepob/J
000696 BKS.Cg-Lepob/J
004824 BTBR.Cg-Lepob/WiscJ
006906 B6.Cg-Lepob Ldlrtm1Her/J
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.