Stock Number:007974
Strain Name:B6;CBA-Tg(Olfr160-taulacZ)V4-7Mom/MomJ
Generic LacZ
Generic LacZ QPCR
QPCR
4.0
16-December -2011 23-May -2012
Notes: Taqman qPCR protocols are run on a real time PCR instrument. Use an appropriate
instrument specific Fluorophore/Quencher combination. The transgene genotype is
determined by comparing ΔCt values of each unknown sample against known
homozygous and hemizygous controls, using appropriate endogenous references.
Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
13742 6-FAM CGG TCA ATC CGC CGT TTG TTC

Zen probe with Iowa Black

 Tg Probe
13745 6-FAM CCA ATG GTC GGG CAC TGC TCA A

Zen probe with Iowa Black

 IC Probe
oIMR1443 - GCC CAT CTA CAC CAA CGT AAC C - Transgene Forward
oIMR1444 - AGT AAC AAC CCG TCG GAT TCT C - Transgene Reverse
oIMR1544 - CAC GTG GGC TCC AGC ATT - Internal Positive Control Forward
oIMR3580 - TCA CCA GTC ATT TCT GCC TTT G - Internal Positive Control Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
ddH2O 0.00 - 0.00
2 X PerfeCTa qPCR SuperMix 6.25 1.00 X 6.25
40 uM oIMR1443 0.13 0.42 uM 0.13
20 uM oIMR1444 0.13 0.21 uM 0.13
40 uM oIMR1544 0.13 0.42 uM 0.13
40 uM oIMR3580 0.13 0.42 uM 0.13
DNA 5.00 - 5.00
5 uM 13745 0.38 0.15 uM 0.38
5 uM 13742 0.38 0.15 uM 0.38
Cycling 
Step # Temp °C Time Note
1 50 2 min -
2 95 10 min -
3 95 15 sec -
4 60 1 min repeat steps 3-4 for 40 cycles
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
002982 B6.Cg-Tg(xstpx-lacZ)32And/J
004623 STOCK Tg(Fos-lacZ)34Efu/J
005317 B6.Cg-Tg(BAT-lacZ)3Picc/J
005707 STOCK Rag1tm1Mom Tg(TIE2-lacZ)182Sato/J
005728 STOCK Tg(tetO-Ipf1,lacZ)958.1Macd/J
007972 B6;CBA-Tg(Olfr151-taulacZ)4Mom/MomJ
007973 B6;CBA-Tg(Olfr16-taulacZ)1Mom/MomJ
007974 B6;CBA-Tg(Olfr160-taulacZ)V4-7Mom/MomJ
007975 B6;CBA-Tg(OR8A1-taulacZ)1Mom/MomJ
007976 B6;CBA-Tg(Olfr713-taulacZ)4Mom/MomJ
008477 STOCK Tg(RARE-Hspa1b/lacZ)12Jrt/J
008618 B6;A-Tg(OPN1LW-lacZ)1Nat/J
012771 129-Sirt7tm1Fwa/J
014536 B6.Cg-Hprttm75(Ple143-lacZ)Ems/Mmjax
304623 Tg(Fos-lacZ)34Efu/J
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.