Stock Number: 008250 Strain Name: STOCK Tg(Ins2-rtTA)2Efr/J

Allele: Prkdcscid Protocol Name: Prkdcscid Method: Pyrosequencing Version: 4.0 Created: 19-May -2009 Updated: 24-September-2009

Notes: This genotyping assay uses pyrosequencing technology and is run on the Biotage PSQ 96MA. The Jackson Laboratory is not posting the complete details of our pyrosequencing genotyping assays as the primers for pyrosequencing cannot be used for sequencing using more traditional methods. The wild type and mutant nucleotides and the flanking DNA sequence are provided below. Expected Results: Mutant = A/A Heterozygote = T/A Wild type = T/T

This genotyping protocol is used for the following strains:   Stock Number Strain Name 001803 CBySmn.CB17-Prkdcscid/J 004644 NOD.Cg Prkdcscid-Tg(CSF2)2Ygy Tg(IL3)1Ygy Tg(KITLG)3Ygy/YgyJ 008168 STOCK Tg(tetO-DTA)1Gfi/J 008250 STOCK Tg(Ins2-rtTA)2Efr/J 009617 NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(HLA-A2.1)1Enge/SzJ 010636 NOD.Cg-Prkdcscid B2mtm1Unc Il2rgtm1Wjl/SzJ 374136 B6.129S4-Ccr2tm1Ifc/J B6.CB17-Prkdcscid/SzJ 374138 B6.129S4-Ccr2tm1Ifc/J B6.129S4-Ccl2tm1Rol/J B6.CB17-Prkdcscid/SzJ 671055 Prkdcscid Blaj Genotyping resources and troubleshooting This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.