Stock Number:012581
Strain Name:B6.129P2(Cg)-Hprttm62(Ple153-lacZ)Ems/Mmjax
Gt(ROSA)26Sortm1Sor
Gt(ROSA)26Sortm1sor STD
Standard PCR
3.0
23-April -2010 05-June -2011
Expected Results:

Mutant = 340 bp
Heterozygote = 340 bp and ~650 bp
Wild type = ~650 bp
Gel Image 
Image Image file
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Gel Information: Separated by gel electrophoresis on a 1.5% agarose gel.
Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
oIMR8052 - GCG AAG AGT TTG TCC TCA ACC - Mutant Reverse
oIMR8545 - AAA GTC GCT CTG AGT TGT TAT - Common
oIMR8546 - GGA GCG GGA GAA ATG GAT ATG - Wild type Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
ddH2O 4.32 - 4.32
10 X KAPA Rxn Buffer 1.20 1.00 X 1.20
2.5 mM dNTP 0.96 0.20 mM 0.96
20 uM oIMR8545 0.60 1.00 uM 0.60
20 uM oIMR8546 0.60 1.00 uM 0.60
20 uM oIMR8052 0.60 1.00 uM 0.60
5 mM DNA Loading Dye 1.66 0.69 mM 1.66
5 U/ul KAPATaq DNA Polymerase 0.06 0.03 U/ul 0.06
DNA 2.00 - 2.00
Cycling 
Step # Temp °C Time Note
1 94 3 min -
2 94 30 sec -
3 65 1 min -
4 72 1 min repeat steps 2-4 for 35 cycles
5 72 2 min -
6 10 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
003309 B6;129S4-Gt(ROSA)26Sortm1Sor/J
003310 129S-Gt(ROSA)26Sortm1Sor/J
003474 B6.129S4-Gt(ROSA)26Sortm1Sor/J
006331 STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
006911 B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J
006953 STOCK Notch1tm3(cre)Rko/J
006965 B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
007670 B6;129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J
007689 129S4/SvJaeSor-Gt(ROSA)26Sortm4(attB/attP)Sor/J
007743 B6.129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J
007844 129S4/SvJae-Gt(ROSA)26Sortm2(FLP*)Sor/J
008706 B6.129P2(Cg)-Hprttm4(Ple88-EGFP)Ems/Mmjax
008707 B6.129P2(Cg)-Hprttm7(Ple185-EGFP)Ems/Mmjax
008708 B6.129P2(Cg)-Hprttm8(Ple151-EGFP)Ems/Mmjax
008709 B6.129P2(129S4)-Hprttm9(Ple178-EGFP/cre)Ems/Mmjax
008710 B6.129P2(129S4)-Hprttm10(Ple162-EGFP/cre)Ems/Mmjax
008876 STOCK Hprttm11(Ple176-EGFP/cre)Ems/Mmjax
008877 B6.129P2(129S4)-Hprttm12(Ple177-EGFP/cre)Ems/Mmjax
008883 B6;129-Gt(ROSA)26Sortm1(SNCA*A53T)Djmo/TmdJ
008886 B6;129-Gt(ROSA)26Sortm3(SNCA*E46K)Djmo/TmdJ
008889 B6;129-Gt(ROSA)26Sortm2(SNCA*119)Djmo/TmdJ
009427 FVB.129S4(B6)-Gt(ROSA)26Sortm1Sor/J
009573 B6(129S4)-Et(cre/ERT2)4Rdav/J
009577 B6(129S4)-Et(cre/ERT2)296Rdav/J
009579 B6(129S4)-Et(cre/ERT2)837Rdav/J
009581 B6(129S4)-Et(cre/ERT2)1642Rdav/J
009582 B6(129S4)-Et(cre/ERT2)1645Rdav/J
009583 B6(129S4)-Et(cre/ERT2)1957Rdav/J
009584 B6(129S4)-Et(cre/ERT2)2007Rdav/J
009585 B6(129S4)-Et(cre/ERT2)2047Rdav/J
009586 B6(129S4)-Et(icre)754Rdav/J
009587 B6(129S4)-Et(icre)1402Rdav/J
009588 B6(129S4)-Et(icre)1470Rdav/J
009589 B6(129S4)-Et(icre)1555Rdav/J
011004 STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J
011011 STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J
011013 STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm5(tetO-Pou5f1,-Klf4,-Myc)Jae/J
012580 B6.129P2(Cg)-Hprttm59(Ple123-lacZ)Ems/Mmjax
012581 B6.129P2(Cg)-Hprttm62(Ple153-lacZ)Ems/Mmjax
012583 B6.129P2(Cg)-Hprttm68(Ple127-lacZ)Ems/Mmjax
012584 STOCK Hprttm69(Ple134-lacZ)Ems/Mmjax
012687 B6(129S4)-Tg(SYN1-icre/mRFP1)9934Rdav/J
012930 B6.129S4-Gt(ROSA)26Sortm2(FLP*)Sor/J
013586 B6.129P2-Gt(ROSA)26Sortm1Nik/J
014588 B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1A1tm6(tetO-MSI2)Jae/J
014602 B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm1(tetO-mCherry)Eggn/J
016977 FVB.129S6-Gt(ROSA)26Sortm1(Pik3ca*H1047R)Egan/J
018903 STOCK Gt(ROSA)26Sortm2(EGFP/cre)Alj/J
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.