Stock Number: 003752 Strain Name: C57BL/10ScNJ

Allele: Tlr4lps-del Protocol Name: Tlr4lps-del Method: Melt Curve Analysis Version: 2.1 Created: 05-May -2009 Updated: 26-October -2009

Notes: Melting curve analysis is done using a Roche Light Cycler 480. Expected Results: wild=upper slope het=middle slope hom=lower slope

Gel Image

no data found

Protocol Primers

Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type oIMR8365 - GCA AGT TTC TAT ATG CAT TCT C - Mutant Forward oIMR8366 - CCT CCA TTT CCA ATA GGT AG - Mutant Reverse oIMR8367 - ATA TGC ATG ATC AAC ACC ACA G - Wild type Forward oIMR8368 - TTT CCA TTG CTG CCC TAT AG - Wild type Reverse

Number of Reactions

Reaction/Components  Reaction Component Volume (µl) Final Concentration Total Volume (µl) ddH2O 2.40 - 2.40 2 X SYBR Green Master Mix (Roche) 6.00 1.00 X 6.00 20 uM oIMR8365 0.40 0.67 uM 0.40 20 uM oIMR8366 0.40 0.67 uM 0.40 20 uM oIMR8367 0.40 0.67 uM 0.40 20 uM oIMR8368 0.40 0.67 uM 0.40 DNA 2.00 - 2.00

Cycling  Step # Temp °C Time Note 1 94 5 min - 2 94 30 sec* - 3 59 1 min - 4 72 30 sec* repeat steps 2-4 for 35 cycles 5 72 2 min - 6 10 - hold

This genotyping protocol is used for the following strains:   Stock Number Strain Name 003752 C57BL/10ScNJ 005315 B6;C3H-Tg(Scgb1a1-Scnn1b)6608Bouc/J 007227 B6.B10ScN-Tlr4lps-del/JthJ Genotyping resources and troubleshooting This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.