Stock Number:008228
Strain Name:C3.129S7(B6)-Ifngtm1Ts/J

Ifngtm1Ts
Ifngtm1Ts
Standard PCR
9.0
11-November -2011 11-November -2011
Expected Results: Mutant = ~500 bp
Heterozygote = ~210 bp and ~500 bp
Wild type = ~210 bp
Gel Image 
Image Image file
View Full Size
- View Full Size
Gel Information: Separated by gel electrophoresis on a 1.5% agarose gel.
Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
oIMR6218 - CCT TCT ATC GCC TTC TTG ACG - Mutant Forward
oIMR8284 - AGA AGT AAG TGG AAG GGC CCA GAA G - Wild type Forward
oIMR8285 - AGG GAA ACT GGG AGA GGA GAA ATA T - Common
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration
Total Volume (µl)
20 uM oIMR8285 0.60 1.00 uM 0.60
20 uM oIMR8284 0.20 0.33 uM 0.20
20 uM oIMR6218 1.40 2.33 uM 1.40
ddH2O 3.46 - 3.46
5 U/ul KAPATaq DNA Polymerase 0.06 0.03 U/ul 0.06
2.5 mM dNTP 0.96 0.20 mM 0.96
10 X KAPA Rxn Buffer 1.66 1.38 X 1.66
5 mM DNA Loading Dye 1.66 0.69 mM 1.66
DNA 2.00 - 2.00
Cycling 
Step # Temp °C Time Note
1 94 3 min -
2 94 30 sec -
3 67 1 min -
4 72 1 min Go to step 2-4 35 times
5 72 2 min -
6 10 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
002286 C.129S7(B6)-Ifngtm1Ts/J
002287 B6.129S7-Ifngtm1Ts/J
002575 NOD.129S7(B6)-Ifngtm1Ts/DvsJ
008228 C3.129S7(B6)-Ifngtm1Ts/J
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.