Stock Number: 008473 Strain Name: B6;SJL-Tg(THY1-SNCA*A30P)M30Sud/J

Allele: Tg(THY1-SNCA*A30P)M30Sud Protocol Name: Tg(THY1-SNCA*A53T)M53Sud, F53Sud Method: Melt Curve Analysis Version: 3.0 Created: 04-September-2009 Updated: 24-September-2009

Expected Results: Transgene = 500 bp IC Tm= 83 +/-1 IC Area=10 Tg/0-IC Tm=83 +/-1 Tg/0-IC Area=0.1 Tg/0 Tm=86.5 +/-1.5 Tg/0 Area=10

Gel Image

no data found

Protocol Primers

Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type oIMR8584 - GGC ACC TAG AGG ATC TCG ACT AGT GG - - oIMR8585 - GGA CCT CGA CGC TTA GGC TTC AGG - - oIMR8744 - CAA ATG TTG CTT GTC TGG TG - Internal Positive Control Forward oIMR8745 - GTC AGT CGA GTG CAC AGT TT - Internal Positive Control Reverse

Number of Reactions

Reaction/Components  Reaction Component Volume (µl) Final Concentration Total Volume (µl) ddH2O 1.24 - 1.24 20 uM oIMR8584 0.60 1.00 uM 0.60 20 uM oIMR8585 0.60 1.00 uM 0.60 DNA 2.00 - 2.00 10 X High Resolution Melt Master Mix (Roche) 6.00 5.00 X 6.00 25 mM MgCl2 0.96 2.00 mM 0.96 20 oIMR8744 0.30 0.50 0.30 20 uM oIMR8745 0.30 0.50 uM 0.30

Cycling  Step # Temp °C Time Note 1 94 10min - 2 94 30 sec - 3 60 1min - 4 72 1 min repeat steps 2-4 for 35 cycles 5 72 2 min - 6 10 - hold**

Reaction/Components  None found. This genotyping protocol is used for the following strains:   Stock Number Strain Name 008132 STOCK Tg(THY1-Snca)M1mSud/J 008135 B6.Cg-Tg(THY1-SNCA*A53T)M53Sud/J 008473 B6;SJL-Tg(THY1-SNCA*A30P)M30Sud/J 008474 STOCK Tg(THY1-SNCA*A53T)F53Sud/J Genotyping resources and troubleshooting This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.

Cycling  None found.