Stock Number:004178
Strain Name:B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J

Generic GFP
Fluorescent Proteins (Generic GFP)
Standard PCR
3.0
17-January -2011 05-June -2011
Notes: This assay will NOT distinguish hemizygous from homozygous transgenic animals.
Expected Results: Transgene = 173 bp
Internal positive control = 324 bp
Gel Image 
Image Image file
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Gel Information: Separated by gel electrophoresis on a 1.5% agarose gel.
Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
oIMR0872 - AAG TTC ATC TGC ACC ACC G - Transgene
oIMR1416 - TCC TTG AAG AAG ATG GTG CG - Transgene
oIMR7338 - CTA GGC CAC AGA ATT GAA AGA TCT - Internal Positive Control Forward
oIMR7339 - GTA GGT GGA AAT TCT AGC ATC ATC C - Internal Positive Control Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration
Total Volume (µl)
5 U/ul KAPATaq DNA Polymerase 0.12 0.05 U/ul 0.12
ddH2O 3.30 - 3.30
25 mM MgCl2 0.96 2.00 mM 0.96
2.5 mM dNTP 0.96 0.20 mM 0.96
20 uM oIMR0872 0.60 1.00 uM 0.60
20 uM oIMR1416 0.60 1.00 uM 0.60
20 uM oIMR7338 0.30 0.50 uM 0.30
20 uM oIMR7339 0.30 0.50 uM 0.30
5 mM DNA Loading Dye 1.66 0.69 mM 1.66
DNA 2.00 - 2.00
10 X KAPA Rxn Buffer 1.20 1.00 X 1.20
Cycling 
Step # Temp °C Time Note
1 94 1.5 min -
2 94 30 sec -
3 60 1 min -
4 72 1 min repeat steps 2-4 for 35 cycles
5 72 2 min -
6 10 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
003115 STOCK Tg(CAG-EGFP)B5Nagy/J
003116 STOCK Tg(CAG-EGFP)D4Nagy/J
003257 FVB/N-Tg(GFAPGFP)14Mes/J
003291 C57BL/6-Tg(CAG-EGFP)1Osb/J
003516 FVB.Cg-Tg(CAG-EGFP)B5Nagy/J
003718 FVB-Tg(GadGFP)45704Swn/J
003773 STOCK Tg(CAG-ECFP)CK6Nagy/J
003920 STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
003927 C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ
004178 B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J
004218 B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J
004353 C57BL/6-Tg(UBC-GFP)30Scha/J
004509 B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
004512 C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
004654 B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J
004690 B6;FVB-Tg(Pcp2-EGFP)2Yuza/J
005029 B6.Cg-Tg(Hlxb9-GFP)1Tmj/J
005070 C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
005104 STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005244 B6.Cg-Tg(Krt1-15-EGFP)2Cot/J
005282 NOD/ShiLtJ-Tg(Ins1-EGFP/GH1)14Hara/HaraJ
005418 STOCK Tg(HIST1H2BB/EGFP)1Pa/J
005483 129-Tg(CAG-EYFP)7AC5Nagy/J
005515 FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
005620 B6;D2-Tg(S100B-EYFP)1Wjt/J
005621 B6;D2-Tg(S100B-EGFP)1Wjt/J
005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J
005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J
005699 STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
005706 C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
005738 B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J
005854 STOCK Tg(Cp-EGFP)25Gaia/J
006043 B6;SJL-Tg(Oxt/EGFP)AI03Wsy/J
006069 B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J
006129 STOCK Tg(Zp3-EGFP)1Dean/J
006361 B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
006567 C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ
006570 STOCK Tg(SMN2)89Ahmb Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj/J
006614 B6;CBA-Tg(Thy1-CFP/COX8A)C1Lich/J
006617 B6;CBA-Tg(Thy1-CFP/COX8A)S2Lich/J
006665 B6;129P2-Olfr151tm13(rI7)Mom/MomJ
006850 STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006864 B6.Cg-Tg(Ins1-EGFP)1Hara/J
007075 CByJ.B6-Tg(CAG-EGFP)1Osb/J
007076 CByJ.B6-Tg(UBC-GFP)30Scha/J
007264 C57BL/6-Tg(Sry-EGFP)92Ei Tg(Sry)4Ei Chr YPOS/EiJ
007265 C57BL/6-Tg(Sry-EGFP)92Ei Chr YAKR/J/EiJ
007483 FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
007484 B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
007567 C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J
007673 B6.Cg-Tg(Gad1-EGFP)3Gfng/J
007742 B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J
007857 C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J
007860 C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J
007894 B6.Cg-Tg(Rgs4-EGFP)4Lvt/J
007940 B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J
007967 B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J
007977 B6;CBA-Tg(Hf/Olfr16-GFP)7Mom/MomJ
007979 B6;CBA-Tg(H/Olfr16-GFP)3Mom/MomJ
007980 B6;CBA-Tg(H/Olfr16-GFP)4Mom/MomJ
007984 B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)11Mom/MomJ
007985 B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)13Mom/MomJ
007986 B6;CBA-Tg(H*/Olfr16-GFP)11Mom/MomJ
007987 B6;CBA-Tg(H*/Olfr16-GFP)25Mom/MomJ
008004 B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J
008126 B6.NOD-Tg(Cd4-EGFP)1Lt/J
008173 NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
008344 B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J
008450 FVB-Tg(CAG-luc,-GFP)L2G85Chco/J
008547 NOD.FVB-Tg(ITGAM-DTR/EGFP)34Lan/JdkJ
008549 NOD.FVB-Tg(Itgax-DTR/EGFP)57Lan/JdkJ
008579 STOCK Tg(PSCA-EGFP)1Witt/J
008694 NOD/ShiLt-Tg(Foxp3-EGFP/cre)1cJbs/J
009159 B6;FVB-Tg(Cnp-EGFP/Rpl10a)JD368Htz/J
009593 C57BL/6J-Tg(Pomc-EGFP)1Low/J
010542 NOD.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
010545 C.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
010713 C57BL/6-Tg(tetO-GFP/tetX)5696Stl/J
010827 B6;C3-Tg(FOXJ1-EGFP)85Leo/J
011106 STOCK Tg(CAG-GFP*)1Hadj/J
012350 B6;SJL-Tg(Thy1-COP4*H134R/EYFP)20Gfng/J
012643 B6.Cg-Tg(Ly6a-EGFP)G5Dzk/J
013134 B6.Cg-Tg(Col1a1*2.3-GFP)1Rowe/J
013233 NOD.B6-Tg(Itgax-cre,-EGFP)4097Ach/J
014135 B6.Cg-Tg(Fos/EGFP)1-3Brth/J
014548 B6.Cg-Tg(Slc32a1-COP4*H134R/EYFP)8Gfng/J
017466 B6.Cg-Tg(Col1a1*3.6-Topaz)2Rowe/J
017467 C57BL/6-Tg(Dmp1-Topaz)1Ikal/J
017468 STOCK Tg(Col1a1*3.6-Cyan)2Rowe/J
017469 C57BL/6-Tg(BGLAP-Topaz)1Rowe/J
017472 STOCK Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)1Pmay/J
017484 FVB-Tg(JPH3-GFP,-JPH3*)GXwy/J
017495 B6;129S7-Crim1tm1(GFP/cre/ERT2)Pzg/J
017592 B6;129S-Sox2tm2Hoch/J
017619 NOD.Cg-Prkdcscid Tg(CAG-EGFP)1Osb/KupwJ
017952 STOCK Tg(Isl1-EGFP*)1Slp/J
018067 FVB-Tg(Prism)1849Htz/J
018068 FVB-Tg(Prism)1989Htz/J
018071 FVB-Tg(Prism)1861Htz/J
018306 B6.Cg-Tg(Fos-tTA,Fos-EGFP*)1Mmay/J
018322 STOCK Tg(Cp-EGFP)25Gaia/ReyaJ
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.