Stock Number: 002760 Strain Name: C57BLKS/J-Npc1spm/J

Allele: Npc1spm Protocol Name: Npc1spm Method: Pyrosequencing Version: 3.0 Created: 06-December -2010 Updated: 06-December -2010

Notes: This genotyping assay uses pyrosequencing technology and is run on the Biotage PSQ 96MA. The Jackson Laboratory is not posting the complete details of our pyrosequencing genotyping assays as the primers for pyrosequencing cannot be used for sequencing using more traditional methods. The wild type and mutant nucleotides and the flanking DNA sequence are provided below. Expected Results: Mutant = T/T Heterozygote = A/T Wild type = A/A

Sequence: TCTGGTCCTTTGTACTTCTTTTTCTCTTTAATTAGCGCTTAGTTTGAGATATGTTCTCCCT GTATGGTCTCCAAGTTGACCATGAACCTGGAGTGGGCCCTATCTCAGCCTCCTGAGTGT TAGGGTTAGACTAGTTCTCGCCACCTGCCTGGGTTTGGTCCTTTTCAATCGTGAACAGA AAGGTAGAAGGGGCTAGAGTGATGGCTCATTGGTTAAGAGCACTGACTGCTCTTCCAA AGGTCCTGAGTTCAATTCCCAGCAACCACGTGGTGGCTCACAGCCATCTGTATTAAATA AATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATCTTTGAGAGAGTGGGG GAAGGAATGAAAGAGAGAAGAGAAGAGAAAGAGGTAGAAGGAGGAACTCGTGGCC AGGATCAACTCAGGCCTGAAATTTCTCACTAAAACCAGATTTTCTTTTGGGAAGGTTG CTTTTTGTTGTCATTTCAAAAAGTGTTAGAGCAAGTTTCCTGTGAGCAAAGTGGGCTC ATTGGCCATGGGTTTTCTTGAAGGTTTGGTCAGTAGTATAGCAGCAGTGTCAAGTGGA GGGAAGGAACTAGAGTTTGCAAGAGTCCCAACAAGCCTGTTACTGTCTCTCCTCTCT CCAGCACCCGAGTAGGCTTCGCCCCCTCGTCCTGGATCGATGACTACTTTGACTGGGT CTCGCCACAGTCGTCCTGCTGCAGACTCTACAACGTCACTCACCAGTTCTGCAATGCT TCTGGT(a/c)CTTCATCTCCTTTTCTAGACTTTCTCATTTCAGAAAAATGGTAGCATCAC TTAGCCATTTCACTTTCCCTTCGTTTGTGCGTGTTTGTCAGTGTTGGAGCCGTCTCCTT CCCTCTGTCATTGTCCTGTGCTCAGTCACACACGTGACTCTCAGAGCTGCGTCAAGC AAGGAGAGCACCAAGGGCTTGGTCCCCGGTCAGACTTCTCATTGGTGCAGAGCACACTTACTGCTT

This genotyping protocol is used for the following strains:   Stock Number Strain Name 002760 C57BLKS/J-Npc1spm/J Genotyping resources and troubleshooting This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.