Stock Number:009606
Strain Name:STOCK Tg(Six2-EGFP/cre)1Amc/J
Generic Cre
Generic Cre Melt Curve Analysis
Melt Curve Analysis
1.1
12-September-2008 25-November -2009
Notes: Melting curve analysis is done using a Roche Light Cycler 480.

This assay will NOT distinguish hemizygous from homozygous transgenic animals.
Expected Results: Transgene Tm = 84°C +/- 1.0°C
Internal positive control Tm = 79.4°C+/- 1.0°C
Gel Image 
Image Image file
View Full Size
Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
oIMR1084 - GCG GTC TGG CAG TAA AAA CTA TC - Transgene
oIMR1085 - GTG AAA CAG CAT TGC TGT CAC TT - Transgene
oIMR7338 - CTA GGC CAC AGA ATT GAA AGA TCT - Internal Positive Control Forward
oIMR7339 - GTA GGT GGA AAT TCT AGC ATC ATC C - Internal Positive Control Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
ddH2O 1.60 - 1.60
2 X SYBR Green Master Mix (Roche) 6.00 1.00 X 6.00
20 uM oIMR1084 0.60 1.00 uM 0.60
20 uM oIMR1085 0.60 1.00 uM 0.60
20 uM oIMR7338 0.60 1.00 uM 0.60
20 uM oIMR7339 0.60 1.00 uM 0.60
DNA 2.00 - 2.00
Cycling 
Step # Temp °C Time Note
1 94 5 min -
2 94 15 sec -
3 65 30 Sec -
4 72 30 sec repeat steps 2-4 for 35 cycles
5 72 2 min -
6 10 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
003377 FVB/N-Tg(Zp3-cre)3Mrt/J
003394 B6.FVB-Tg(Zp3-cre)3Mrt/J
003465 BALB/c-Tg(CMV-cre)1Cgn/J
003466 B6;D2-Tg(Sycp1-cre)4Min/J
003554 B6;SJL-Tg(Col2a1-cre)1Bhr/J
003556 B6.Cg-Tg(Mx1-cre)1Cgn/J
003573 B6.Cg-Tg(Ins2-cre)25Mgn/J
003574 B6.Cg-Tg(Alb-cre)21Mgn/J
003651 C57BL/6-Tg(Zp3-cre)93Knw/J
003724 B6.FVB-Tg(EIIa-cre)C5379Lmgd/J
003734 B6;FVB-Tg(GZMB-cre)1Jcb/J
003771 B6.Cg-Tg(Nes-cre)1Kln/J
003802 B6.Cg-Tg(Lck-cre)548Jxm/J
003829 STOCK Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J
003855 NOD/ShiLt-Tg(Ins2-cre)5Lt/LtJ
003966 B6.Cg-Tg(Syn1-cre)671Jxm/J
003967 B6.Cg-Tg(Rbp3-cre)528Jxm/J
004128 B6.Cg-Tg(Tek-cre)12Flv/J
004426 B6;SJL-Tg(Cga-cre)3Sac/J
004453 STOCK Tg(CAG-cre/Esr1*)5Amc/J
004682 B6.Cg-Tg(CAG-cre/Esr1*)5Amc/J
004783 STOCK Tg(Sox2-cre)1Amc/J
004986 NOD/ShiLt-Tg(Ins2-cre)3Lt/LtJ
005025 FVB.Cg-Tg(SMN2*delta7)4299Ahmb Tg(SMN2)89Ahmb Smn1tm1Msd/J
005069 B6.Cg-Tg(Fabp4-cre)1Rev/J
005105 STOCK Tg(Chx10-EGFP/cre,-ALPP)2Clc/J
005249 B6;SJL-Tg(Krt1-15-cre/PGR)22Cot/J
005584 B6.Cg-Tg(Prrx1-cre)1Cjt/J
005628 B6.129S2-Emx1tm1(cre)Krj/J
005667 STOCK Tg(Neurog3-cre)C1Able/J
005673 C.Cg-Tg(Mx1-cre)1Cgn/J
005697 B6.129-Otx1tm4(cre)Asim/J
005938 STOCK Tg(Eno2-cre)39Jme/J
005965 STOCK Tg(Pomc1-cre)16Lowl/J
005975 B6.Cg-Tg(Plp1-cre/ERT)3Pop/J
005989 129;FVB-Tg(PTH-cre)4167Slib/J
005994 STOCK Mbtps1tm1Jdh/J
006054 B6.C-Tg(CMV-cre)1Cgn/J
006137 B6.Cg-Tg(Cdh5-cre)7Mlia/J
006139 FVB.Cg-Tg(ACTA1-cre)79Jme/J
006143 FVB/N-Tg(Thy1-cre)1Vln/J
006149 B6.Cg-Tg(ACTA1-cre)79Jme/J
006207 STOCK Tg(Pcp2-cre)1Amc/J
006224 STOCK Tg(tetO-cre)1Jaw/J
006230 B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J
006234 B6.Cg-Tg(tetO-cre)1Jaw/J
006244 C.Cg-Tg(tetO-cre)1Jaw/J
006297 FVB.Cg-Tg(Eno2-cre)39Jme/J
006333 B6.FVB(Cg)-Tg(Neurog3-cre)C1Able/J
006361 B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
006368 B6.Cg-Tg(Cr2-cre)3Cgn/J
006395 STOCK Tg(Sim1-cre)1Lowl/J
006447 B6.129S6(CBA)-Cebpatm1Dgt/J
006451 B6.FVB(129X1)-Tg(Sim1-cre)1Lowl/J
006474 C57BL/6-Tg(Grik4-cre)G32-4Stl/J
006600 B6.129S1-Mnx1tm4(cre)Tmj/J
006663 B6.Cg-Tg(Eno2-cre)39Jme/J
006922 B6.Cg-Sfpi1tm2Dgt/J
007001 B6;129S-Tg(UBC-cre/ERT2)1Ejb/J
007179 129S.Cg-Tg(UBC-cre/ERT2)1Ejb/J
007252 B6Ei.129S4-Tg(Prm-cre)58Og/EiJ
007567 C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
007673 B6.Cg-Tg(Gad1-EGFP)3Gfng/J
007684 STOCK Tg(Atoh1-cre/Esr1*)14Fsh/J
007742 B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J
007913 STOCK Gli1tm3(cre/ERT2)Alj/J
007915 129S.FVB-Tg(Amh-cre)8815Reb/J
008069 B6;129P2-Pvalbtm1(cre)Arbr/J
008085 B6.Cg-Tg(UBC-cre/ERT2)1Ejb/J
008205 B6.Cg-Tg(NPHS2-cre)295Lbh/J
008244 FVB.Cg-Tg(tetO-cre)1Jaw/J
008320 B6.129-Leprtm2(cre)Rck/J
008397 B6.129-Pcyt1atm1Irt/J
008454 B6.Cg-Tg(Sox2-cre)1Amc/J
008533 B6;FVB-Tg(Cspg4-cre)1Akik/J
008582 STOCK Tg(Kcnc2-Cre)K128Stl/LetJ
008601 B6.Cg-Tg(Th-cre)1Tmd/J
008694 NOD/ShiLt-Tg(Foxp3-EGFP/cre)1cJbs/J
008783 STOCK Smn1tm3(SMN2/Smn1)Mrph Tg(SMN2*delta7)4299Ahmb Tg(SMN2)89Ahmb Tg(CAG-cre/Esr1*)5Amc/J
008839 B6;C3-Tg(Cyp39a1-cre)1Aibs/J
008844 B6;C3-Tg(Ctgf-cre)2Aibs/J
008851 STOCK Tg(Wnt1-cre/ERT)1Alj/J
008852 STOCK Tg(En2-cre)22Alj/J
008863 B6.Cg-Tg(Tek-cre)1Ywa/J
008870 C57BL/6-Tg(Hspa2-cre)1Eddy/J
009074 STOCK Tg(Myh6-cre)1Jmk/J
009102 STOCK Tg(Nefh-cre)12Kul/J
009103 B6;C3-Tg(Wfs1-cre/ERT2)3Aibs/J
009111 B6;C3-Tg(Scnn1a-cre)1Aibs/J
009112 B6;C3-Tg(Scnn1a-cre)2Aibs/J
009117 B6;C3-Tg(Cyp39a1-cre)7Aibs/J
009155 C57BL/6-Cldn6tm1(cre)Dkwu/J
009350 B6.Cg-Tg(CDX2-cre)101Erf/J
009352 B6.Cg-Tg(CDX2-cre*)189Erf/J
009388 B6;129S1-Osr2tm2(cre)Jian/J
009573 B6(129S4)-Et(cre/ERT2)4Rdav/J
009574 B6(129S4)-Et(cre/ERT2)21Rdav/J
009575 B6(129S4)-Et(cre/ERT2)119Rdav/J
009576 B6;129S4-Et(cre/ERT2)278Rdav/J
009577 B6(129S4)-Et(cre/ERT2)296Rdav/J
009578 B6(129S4)-Et(cre/ERT2)398Rdav/J
009579 B6(129S4)-Et(cre/ERT2)837Rdav/J
009580 B6(129S4)-Et(cre/ERT2)1382Rdav/J
009581 B6(129S4)-Et(cre/ERT2)1642Rdav/J
009582 B6(129S4)-Et(cre/ERT2)1645Rdav/J
009583 B6(129S4)-Et(cre/ERT2)1957Rdav/J
009584 B6(129S4)-Et(cre/ERT2)2007Rdav/J
009585 B6(129S4)-Et(cre/ERT2)2047Rdav/J
009600 B6;129-Six2tm3(EGFP/cre/ERT2)Amc/J
009602 B6.129S4(Cg)-Kcnn2tm2Jpad/J
009606 STOCK Tg(Six2-EGFP/cre)1Amc/J
009613 B6;C3-Tg(Scnn1a-cre)3Aibs/J
009614 B6.Cg-Tg(Wfs1-cre/ERT2)2Aibs/J
009615 STOCK Tg(Cartpt-cre)1Aibs/J
009616 B6;C3-Tg(A930038C07Rik-cre)4Aibs/J
009642 B6.Cg(129)-Tg(Gh1-cre)1Sac/J
010708 B6(Cg)-Ssttm1(cre/ERT2)Zjh/J
010721 STOCK Tgfb1tm2.1Doe/J
010724 C57BL/6-Trim21tm1Hm/J
010803 B6;FVB-Tg(Adipoq-cre)1Evdr/J
011069 B6.FVB-Tg(Gh1-cre)bKnmn/J
012328 B6.Cg-Tg(Tyr-cre/ERT2)13Bos/J
012362 B6;129S6-Tg(Camk2a-cre/ERT2)1Aibs/J
012620 STOCK Trp53tm1Brd Brca1tm1Aash Tg(LGB-cre)74Acl/J
012686 C57BL/6N-Tg(Ppp1r2-cre)4127Nkza/J
012712 B6.Cg-Tg(Fev-cre)1Esd/J
012886 B6.Cg-Tg(Gfap-cre)73.12Mvs/J
013086 B6.Cg-Rxratm1Krc/J
013148 C57BL/6-Tg(Pdgfra-cre)1Clc/J
013251 NOD.FVB-Tg(EIIa-cre)C5379Lmgd/J
013590 B6.Cg-Braftm1Mmcm Ptentm1Hwu Tg(Tyr-cre/ERT2)13Bos/BosJ
013756 C.129S-Batf3tm1Kmm/J
014094 B6N.Cg-Tg(Sox2-cre)1Amc/J
016097 C57BL/6-Tg(Car1-cre)5Flt/J
016132 B6.129S4-Perptm2Att/J
016210 B6;129-Lrrk2tm3.1Shn/J
016261 C57BL/6-Tg(Nes-cre/ERT2)KEisc/J
016881 B6N.Cg-Gpr124tm1.1Bstc/J
016934 B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
016974 FVB;129P2-Mettm1Sst/J
017298 STOCK Hmgn5tm1Mbus/J
017336 STOCK Tg(Cd4-cre)1Cwi/BfluJ
017346 B6.Cg-Tg(A930038C07Rik-cre)1Aibs/J
017491 B6.Cg-Tg(Tagln-cre)1Her/J
017563 B6;129-Fbxw7tm1Iaai/J
017593 B6;129S-Sox2tm1(cre/ERT2)Hoch/J
017601 B6;129-Eif2s1tm1Rjk/J
017678 FVB;129-Pink1tm1Aub Tg(Prnp-SNCA*A53T)AAub/J
017704 B6N.129-Pik3catm1Jjz/J
017738 B6;SJL-Tg(Foxl1-cre)1Khk/J
017741 B6N.129(Cg)-Mecp2tm1.1Joez/J
017789 129S-Slc11a2tm2Nca/J
017836 STOCK Tg(LGB-cre)74Acl/J
017889 B6(Cg)-Shank3tm1.1Bux/J
017948 STOCK Tg(KRT18-cre/ERT2)23Blpn/J
017977 B6.129-Ulk2tm1Thsn/J
018019 B6;129S4-Srsf2tm1Xdfu/J
018054 B6.129P2(Cg)-Pitx2tm1.1Dmm/J
018057 B6.129(Cg)-Cul7tm2.1Jdec/J
018063 B6.129-Fth1tm1.1Lck/J
018319 B6;129-Plxnd1tm1.1Tmj/J
018394 FVB.Cg-Tg(KRT5-cre/ERT2)2Ipc/JeldJ
018402 B6.FVB-Nrn1tm1.2Ndiv/J
018418 B6.129S6(Cg)-Lrig1tm1.1(cre/ERT2)Rj/J
018445 B6.129-Mre11atm1.1Rchd/J
018569 B6N(Cg)-Arid3atm1b(KOMP)Wtsi/J
018773 129-Sult2b1tm1Rus/J
018829 STOCK Tg(Sox9-cre/ERT2)1Msan/J
018937 B6;C-Tg(Erv4-cre/ERT2*)657Slp/J
018961 B6N.Cg-Tg(Alb-cre)21Mgn/J
018997 B6.129S(FVB)-Lhx3tm1.1Sjr/J
019103 B6N.Cg-Tg(Nes-cre)1Kln/CjDswJ
019380 STOCK Tg(IVL-cre/ERT2)1Blpn/J
021160 B6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT)Litt/WganJ
021582 C57BL/6J-Tg(Mchr1-cre)1Emf/J
021614 B6.Cg-Tg(S100A8-cre,-EGFP)1Ilw/J
021752 B6N(Cg)-Tpgs2tm1.1(KOMP)Vlcg/J
1000075 STOCK Tg(Myh6-cre/Esr1*)1Jmk/J (also maybe)
370814 (B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J x C57BL/6 ?Gt(ROSA)26Sortm3(CAG-MIR17-92,-EGFP)Rsky/J) x (B6.Cg-Tg(Tek-cre)1Ywa/J x C57BL/6-Gt(ROSA)26Sortm3(CAG-MIR17-92,-EGFP)Rsky/J)
671224 B6.129-Ulk1tm1Thsn/J x B6.Cg-Tg(Tek-cre)1Ywa/J (17976 x 8863)
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.