Stock Number: 016235 Strain Name: B6(Cg)-Narfltm1.1Fsl/J

Allele: Narfltm1.1Fsl Protocol Name: Narfltm1.1Fsl Method: Standard PCR Version: 1.0 Created: 15-April -2011 Updated: 13-September-2011

Gel Image

Image Image file View Full Size

Protocol Primers

Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type 12235 - CCT GGG ACC ATG GGA GTG TTT AG - - 12236 - GGC GGT CTG TCT GTA TCC CTC TC - -

Number of Reactions

Reaction/Components  Reaction Component Volume (µl) Final Concentration Total Volume (µl) ddH2O 4.92 - 4.92 5 mM DNA Loading Dye 1.66 0.69 mM 1.66 DNA 2.00 - 2.00 2.5 mM dNTP 0.96 0.20 mM 0.96 10 X KAPA Rxn Buffer 1.20 1.00 X 1.20 20 uM 12235 0.60 1.00 uM 0.60 20 uM 12236 0.60 1.00 uM 0.60 5 U/ul KAPATaq DNA Polymerase 0.06 0.03 U/ul 0.06

Cycling  Step # Temp °C Time Note 1 94 3 min - 2 94 30 sec - 3 62 30sec - 4 72 30sec - 5 - - Go to step 2-4, 35 times 6 72 2 min - 7 10 - hold

This genotyping protocol is used for the following strains:   Stock Number Strain Name 016235 B6(Cg)-Narfltm1.1Fsl/J Genotyping resources and troubleshooting This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.