Stock Number:005964
Strain Name:B6.Cg-Tg(GFAP-tTA)110Pop/J
Generic tTA
Tg(tTA)
Melt Curve Analysis
4.1
08-October -2008 24-September-2009
Notes:

Melting curve analysis is done using a Roche Light Cycler 480.

This assay will NOT distinguish hemizygous from homozygous transgenic animals.
Expected Results: Transgene Tm = 92°C +/- 1.0°C
Internal positive control Tm = 82°C+/- 1.0°C
Gel Image 
Image Image file
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Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
oIMR8744 - CAA ATG TTG CTT GTC TGG TG - Internal Positive Control Forward
oIMR8745 - GTC AGT CGA GTG CAC AGT TT - Internal Positive Control Reverse
oIMR8746 - CGC TGT GGG GCA TTT TAC TTT AG - Transgene Forward
oIMR8747 - CAT GTC CAG ATC GAA ATC GTC - Transgene Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
ddH2O 2.60 - 2.60
2 X SYBR Green Master Mix (Roche) 6.00 1.00 X 6.00
20 ul oIMR8744 0.10 0.17 ul 0.10
20 uM oIMR8745 0.10 0.17 uM 0.10
20 uM oIMR8746 0.60 1.00 uM 0.60
20 uM oIMR8747 0.60 1.00 uM 0.60
DNA 2.00 - 2.00
Cycling 
Step # Temp °C Time Note
1 94 5 min -
2 94 30 sec -
3 59 1 min -
4 72 45 sec repeat steps 2-4 for 35 cycles
5 72 2 min -
6 10 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
002618 B6.Cg-Tg(MMTVtTA)1Mam/J
003010 B6;CBA-Tg(Camk2a-tTA)1Mmay/J
003170 FVB.Cg-Tg(Myh6-tTA)6Smbf/J
003271 STOCK Tg(CMV-tTA)3Bjd/J
003272 STOCK Tg(Cebpb-tTA)5Bjd/J
003273 STOCK Tg(CMV-rtTA)4Bjd/J
003563 B6.Cg-Tg(Cebpb-tTA)5Bjd/J
003763 B6.Cg-Tg(Eno2tTA)5030Nes/J
003767 B6.Cg-Tg(Eno2tTA)5021Nes/J
004127 FVB-Tg(Nes-rtTA)306Rvs/J
004937 NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ
005493 STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J
005625 FVB-Tg(Pcp2-tTA)3Horr/J
005734 NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ
005942 FVB/N-Tg(Pf4-tTA/VP16)42Kra/J
005964 B6.Cg-Tg(GFAP-tTA)110Pop/J
006209 FVB.Cg-Tg(Tal1-tTA)19Dgt/J
006222 FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
006225 FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J
006232 B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
006235 B6.Cg-Tg(SFTPC-rtTA)5Jaw/J
006242 C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
006245 C.Cg-Tg(SFTPC-rtTA)5Jaw/J
006875 FVB/N-Tg(Tagln-rtTA)E1Jwst/J
006999 STOCK Dbttm1Geh Tg(Cebpb-tTA)5Bjd Tg(tetO-DBT)A1Geh/J
007004 B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ
007678 B6;SJL-Tg(KRT14-rtTA)208Jek/J
008250 STOCK Tg(Ins2-rtTA)2Efr/J
008790 STOCK Tg(tetO-DISC1*)1001Plet/J
013585 FVB-Tg(Cdh5-tTA)D5Lbjn/J
013729 C57BL/6-Tg(tetO-EDN1,-lacZ)9Mhus/J
016198 129S6.Cg-Tg(Camk2a-tTA)1Mmay/JlwsJ
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.