Generic GFP
Fluorescent Proteins (Generic GFP)
Melt Curve Analysis
3.0
04-June -2009 05-June -2011
Notes: Melting curve analysis is done using a Roche Light Cycler 480.

This assay will NOT distinguish hemizygous from homozygous transgenic animals.
Expected Results: Transgene Tm = 89.5°C +/- 1.0°C
Internal positive control Tm = 79.4°C +/- 1.0°C
Gel Image 
Image Image file
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Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
oIMR0872 - AAG TTC ATC TGC ACC ACC G - Transgene
oIMR3999 - TGC TCA GGT AGT GGT TGT CG - Transgene
oIMR7338 - CTA GGC CAC AGA ATT GAA AGA TCT - Internal Positive Control Forward
oIMR7339 - GTA GGT GGA AAT TCT AGC ATC ATC C - Internal Positive Control Reverse
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
ddH2O 2.00 - 2.00
2 X SYBR Green Master Mix (Roche) 6.00 1.00 X 6.00
20 uM oIMR0872 0.80 1.33 uM 0.80
20 uM oIMR3999 0.80 1.33 uM 0.80
20 uM oIMR7338 0.20 0.33 uM 0.20
20 uM oIMR7339 0.20 0.33 uM 0.20
DNA 2.00 - 2.00
Cycling 
Step # Temp °C Time Note
1 94 5 -
2 94 15 sec -
3 68 1 min -
4 72 30 sec repeat steps 2-4 for 35 cycles
5 72 2 min -
6 10 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
003115 STOCK Tg(CAG-EGFP)B5Nagy/J
003116 STOCK Tg(CAG-EGFP)D4Nagy/J
003257 FVB/N-Tg(GFAPGFP)14Mes/J
003291 C57BL/6-Tg(CAG-EGFP)1Osb/J
003920 STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
004178 B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J
004353 C57BL/6-Tg(UBC-GFP)30Scha/J
004509 B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
004512 C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
004690 B6;FVB-Tg(Pcp2-EGFP)2Yuza/J
005029 B6.Cg-Tg(Hlxb9-GFP)1Tmj/J
005070 C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
005104 STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005282 NOD/ShiLtJ-Tg(Ins1-EGFP/GH1)14Hara/HaraJ
005418 STOCK Tg(HIST1H2BB/EGFP)1Pa/J
005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J
005706 C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
005738 B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J
005854 STOCK Tg(Cp-EGFP)25Gaia/J
006000 B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
006043 B6;SJL-Tg(Oxt/EGFP)AI03Wsy/J
006069 B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J
006129 STOCK Tg(Zp3-EGFP)1Dean/J
006361 B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
006567 C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ
006614 B6;CBA-Tg(Thy1-CFP/COX8A)C1Lich/J
006617 B6;CBA-Tg(Thy1-CFP/COX8A)S2Lich/J
006665 B6;129P2-Olfr151tm13(rI7)Mom/MomJ
006686 STOCK Olfr6tm1Mom/MomJ
006850 STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
007075 CByJ.B6-Tg(CAG-EGFP)1Osb/J
007264 C57BL/6-Tg(Sry-EGFP)92Ei Tg(Sry)4Ei Chr YPOS/EiJ
007567 C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
007572 B6.129P2(Cg)-Rorctm2Litt/J
007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J
007673 B6.Cg-Tg(Gad1-EGFP)3Gfng/J
007677 CB6-Tg(Gad1-EGFP)G42Zjh/J
007857 C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J
007860 C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J
007894 B6.Cg-Tg(Rgs4-EGFP)4Lvt/J
007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J
007940 B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J
007967 B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J
007977 B6;CBA-Tg(Hf/Olfr16-GFP)7Mom/MomJ
007978 B6;CBA-Tg(Hf/Olfr16-GFP)47Mom/MomJ
007979 B6;CBA-Tg(H/Olfr16-GFP)3Mom/MomJ
007981 B6;CBA-Tg(H/Olfr16-GFP)6Mom/MomJ
007982 B6;CBA-Tg(H/Olfr16-taumRFP,-tauGFP)8Mom/MomJ
007984 B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)11Mom/MomJ
007985 B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)13Mom/MomJ
007986 B6;CBA-Tg(H*/Olfr16-GFP)11Mom/MomJ
007987 B6;CBA-Tg(H*/Olfr16-GFP)25Mom/MomJ
008126 B6.NOD-Tg(Cd4-EGFP)1Lt/J
008173 NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
008344 B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J
008450 FVB-Tg(CAG-luc,-GFP)L2G85Chco/J
008549 NOD.FVB-Tg(Itgax-DTR/EGFP)57Lan/JdkJ
008605 B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J
008694 NOD/ShiLt-Tg(Foxp3-EGFP/cre)1cJbs/J
008828 B6.Cg-Tg(Prdm1-EYFP)1Mnz/J
009113 B6.129P2(Cg)-Hprttm13(Ple54-EGFP)Ems/Mmjax
009159 B6;FVB-Tg(Cnp-EGFP/Rpl10a)JD368Htz/J
009353 B6.129P2(Cg)-Hprttm20(Ple53-EGFP)Ems/Mmjax
009422 NOD.Cg-Tg(Itgax-Venus)1Mnz/QtngJ
009593 C57BL/6J-Tg(Pomc-EGFP)1Low/J
009600 B6;129-Six2tm3(EGFP/cre/ERT2)Amc/J
009606 STOCK Tg(Six2-EGFP/cre)1Amc/J
010542 NOD.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
010713 C57BL/6-Tg(tetO-GFP/tetX)5696Stl/J
010774 B6(Cg)-Calb2tm1(cre)Zjh/J
010789 B6.129P2(Cg)-Hprttm54(Ple233-EGFP)Ems/Mmjax
011106 STOCK Tg(CAG-GFP*)1Hadj/J
012350 B6;SJL-Tg(Thy1-COP4*H134R/EYFP)20Gfng/J
012643 B6.Cg-Tg(Ly6a-EGFP)G5Dzk/J
013134 B6.Cg-Tg(Col1a1*2.3-GFP)1Rowe/J
014548 B6.Cg-Tg(Slc32a1-COP4*H134R/EYFP)8Gfng/J
015808 B6.Cg-Tg(UBC-TVA)1Clc/J
016251 129S.Cg-Tg(Hoxb7-EGFP)33Cos/J
016925 129;B6-Grin3b/Tmem259tm1Zhang Tg(Prnp-C19ORF6,-GFP)6Zhang/J
017466 B6.Cg-Tg(Col1a1*3.6-Topaz)2Rowe/J
017468 STOCK Tg(Col1a1*3.6-Cyan)2Rowe/J
017469 C57BL/6-Tg(BGLAP-Topaz)1Rowe/J
017472 STOCK Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)1Pmay/J
017484 FVB-Tg(JPH3-GFP,-JPH3*)GXwy/J
017952 STOCK Tg(Isl1-EGFP*)1Slp/J
018071 FVB-Tg(Prism)1861Htz/J
018148 STOCK Tg(Slc17a8-EGFP)1Edw/SealJ
018549 B6N.Cg-Tg(Csf1r-EGFP)1Hume/J
020942 STOCK Tg(Cp-HIST1H2BB/Venus)47Hadj/J
021187 FVB-Tg(Pbsn-rtTA*M2)42Xy/J
350104 NOD/ShiLt-Tg(Foxp3-EGFP/cre)1Jbs/J
350105 NOD/ShiLt-Tg(Foxp3-EGFP/cre)1Jbs/J
350107 Foxp3-GFP
370368 PAX7_NLS_GFP_T2A_MER_CRE_MER BAC
671282 GcG (TG37)
671283 Drd1a (CP73)
671284 Drd2 (CP101)
671285 GcG (G6)
671286 GcG (G15)
671287 Neurod1
671288 Slc17a6
671290 GcG (TG37)
910681 ND32
911880 ST699
911885 G6
911889 ST826
911980 Glt25d2 (DU9)-F16
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.