Stock Number:004779
Strain Name:STOCK Mapttm1(EGFP)Klt/J
Mapttm1(EGFP)Klt
Mapttm1(EGFP)Klt
Standard PCR
4.0
09-September-2011 09-September-2011
Expected Results:

Mutant = ~490 bp
Heterozygote = 187 bp and ~490 bp
Wild type = 187 bp
Gel Image 
Image Image file
View Full Size
Gel Information: Separated by gel electrophoresis on a 1.5% agarose gel.
Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
oIMR0872 - AAG TTC ATC TGC ACC ACC G - Mutant
oIMR3092 - CTC AGC ATC CCA CCT GTA AC - Wild type
oIMR3093 - CCA GTT GTG TAT GTC CAC CC - Wild type
oIMR3999 - TGC TCA GGT AGT GGT TGT CG - Mutant
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
ddH2O 3.76 - 3.76
25 mM MgCl2 0.96 2.00 mM 0.96
2.5 mM dNTP 0.96 0.20 mM 0.96
20 uM oIMR3092 0.30 0.50 uM 0.30
20 uM oIMR3093 0.30 0.50 uM 0.30
20 uM oIMR0872 0.60 1.00 uM 0.60
20 uM oIMR3999 0.60 1.00 uM 0.60
DNA 2.00 - 2.00
5 X KAPA LR HotStart Buffer 2.40 1.00 X 2.40
2.5 U/ul KAPA LR HotStart Taq 0.12 0.03 U/ul 0.12
Cycling 
Step # Temp °C Time Note
1 94 3 min -
2 94 30 sec -
3 66.8 1 min -
4 72 1 min repeat steps 2-4 for 35 cycles
5 72 2 min -
6 10 - hold**
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
004779 STOCK Mapttm1(EGFP)Klt/J
004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
005491 B6.Cg-Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.