Stock Number: 013148 Strain Name: C57BL/6-Tg(Pdgfra-cre)1Clc/J

Allele: Generic Cre Protocol Name: Generic Cre Method: Standard PCR Version: 1.1 Created: 09-January -2001 Updated: 24-September-2009

Notes: This assay will NOT distinguish hemizygous from homozygous transgenic animals. Expected Results: Transgene = ~100 bp Internal positive control = 324 bp

Gel Image

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Gel Information: Separated by gel electrophoresis on a 3.0% agarose gel.

Protocol Primers

Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type oIMR1084 - GCG GTC TGG CAG TAA AAA CTA TC - Transgene oIMR1085 - GTG AAA CAG CAT TGC TGT CAC TT - Transgene oIMR7338 - CTA GGC CAC AGA ATT GAA AGA TCT - Internal Positive Control Forward oIMR7339 - GTA GGT GGA AAT TCT AGC ATC ATC C - Internal Positive Control Reverse

Number of Reactions

Reaction/Components  Reaction Component Volume (µl) Final Concentration Total Volume (µl) ddH2O 2.79 - 2.79 10 X AB PCR BufferII 1.20 1.00 X 1.20 25 mM MgCl2 0.96 2.00 mM 0.96 2.5 mM dNTP 0.96 0.20 mM 0.96 20 uM oIMR1084 0.60 1.00 uM 0.60 20 uM oIMR1085 0.60 1.00 uM 0.60 20 uM oIMR7338 0.60 1.00 uM 0.60 20 uM oIMR7339 0.60 1.00 uM 0.60 5 mM DNA Loading Dye 1.66 0.69 mM 1.66 5 U/ul Taq DNA Polymerase 0.03 0.01 U/ul 0.03 DNA 2.00 - 2.00

Cycling  Step # Temp °C Time Note 1 94 3 min - 2 94 30 sec - 3 51.7 1 min - 4 72 1 min repeat steps 2-4 for 35 cycles 5 72 2 min - 6 10 - hold

This genotyping protocol is used for the following strains:   Stock Number Strain Name 002471 STOCK Tg(hCMV-cre)140Sau/J 002527 STOCK Tg(Mx1-cre)1Cgn/J 002858 STOCK Tg(Nes-cre)1Wme/J 002859 STOCK Tg(Nes-cre)2Wme/J 003314 FVB/N-Tg(EIIa-cre)C5379Lmgd/J 003328 129S/Sv-Tg(Prm-cre)58Og/J 003376 FVB/N-Tg(ACTB-cre)2Mrt/J 003377 FVB/N-Tg(Zp3-cre)3Mrt/J 003394 B6.FVB-Tg(Zp3-cre)3Mrt/J 003465 BALB/c-Tg(CMV-cre)1Cgn/J 003466 B6;D2-Tg(Sycp1-cre)4Min/J 003553 STOCK Tg(MMTV-cre)4Mam/J 003554 B6;SJL-Tg(Col2a1-cre)1Bhr/J 003556 B6.Cg-Tg(Mx1-cre)1Cgn/J 003573 B6.Cg-Tg(Ins2-cre)25Mgn/J 003574 B6.Cg-Tg(Alb-cre)21Mgn/J 003651 C57BL/6-Tg(Zp3-cre)93Knw/J 003724 B6.FVB-Tg(EIIa-cre)C5379Lmgd/J 003734 B6;FVB-Tg(GZMB-cre)1Jcb/J 003771 B6.Cg-Tg(Nes-cre)1Kln/J 003802 B6.Cg-Tg(Lck-cre)548Jxm/J 003829 STOCK Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J 003855 NOD/ShiLt-Tg(Ins2-cre)5Lt/LtJ 003960 129S6-Tg(Prnp-GFP/cre)1Blw/J 003966 B6.Cg-Tg(Syn1-cre)671Jxm/J 003967 B6.Cg-Tg(Rbp3-cre)528Jxm/J 004128 B6.Cg-Tg(Tek-cre)12Flv/J 004146 B6.129-Tg(Pcp2-cre)2Mpin/J 004426 B6;SJL-Tg(Cga-cre)3Sac/J 004453 STOCK Tg(CAG-cre/Esr1*)5Amc/J 004682 B6.Cg-Tg(CAG-cre/Esr1*)5Amc/J 004746 STOCK Tg(Tagln-cre)1Her/J 004782 STOCK Tg(KRT14-cre)1Amc/J 004783 STOCK Tg(Sox2-cre)1Amc/J 004986 NOD/ShiLt-Tg(Ins2-cre)3Lt/LtJ 004987 NOD/ShiLt-Tg(Ins2-cre)6Lt/LtJ 005069 B6.Cg-Tg(Fabp4-cre)1Rev/J 005105 STOCK Tg(Chx10-EGFP/cre,-ALPP)2Clc/J 005107 STOCK Tg(KRT14-cre/ERT)20Efu/J 005249 B6;SJL-Tg(Krt1-15-cre/PGR)22Cot/J 005359 B6.Cg-Tg(Camk2a-cre)T29-1Stl/J 005584 B6.Cg-Tg(Prrx1-cre)1Cjt/J 005628 B6.129S2-Emx1tm1(cre)Krj/J 005650 STOCK Tg(Myh6-cre/Esr1*)1Jmk/J 005667 STOCK Tg(Neurog3-cre)C1Able/J 005673 C.Cg-Tg(Mx1-cre)1Cgn/J 005697 B6.129-Otx1tm4(cre)Asim/J 005732 NOD.Cg-Tg(Lck-cre)548Jxm/AchJ 005938 STOCK Tg(Eno2-cre)39Jme/J 005965 STOCK Tg(Pomc1-cre)16Lowl/J 005975 B6.Cg-Tg(Plp1-cre/ERT)3Pop/J 005989 129;FVB-Tg(PTH-cre)4167Slib/J 006054 B6.C-Tg(CMV-cre)1Cgn/J 006137 B6.Cg-Tg(Cdh5-cre)7Mlia/J 006139 FVB.Cg-Tg(ACTA1-cre)79Jme/J 006143 FVB/N-Tg(Thy1-cre)1Vln/J 006149 B6.Cg-Tg(ACTA1-cre)79Jme/J 006207 STOCK Tg(Pcp2-cre)1Amc/J 006224 STOCK Tg(tetO-cre)1Jaw/J 006230 B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J 006234 B6.Cg-Tg(tetO-cre)1Jaw/J 006244 C.Cg-Tg(tetO-cre)1Jaw/J 006297 FVB.Cg-Tg(Eno2-cre)39Jme/J 006333 B6.FVB(Cg)-Tg(Neurog3-cre)C1Able/J 006361 B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J 006368 B6.Cg-Tg(Cr2-cre)3Cgn/J 006395 STOCK Tg(Sim1-cre)1Lowl/J 006451 B6.FVB(129X1)-Tg(Sim1-cre)1Lowl/J 006474 C57BL/6-Tg(Grik4-cre)G32-4Stl/J 006600 B6.129S1-Mnx1tm4(cre)Tmj/J 006663 B6.Cg-Tg(Eno2-cre)39Jme/J 006774 FVB-Tg(Col2a1-cre/ERT)KA3Smac/J 007001 B6;129S-Tg(UBC-cre/ERT2)1Ejb/J 007179 129S.Cg-Tg(UBC-cre/ERT2)1Ejb/J 007252 B6Ei.129S4-Tg(Prm-cre)58Og/EiJ 007567 C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J 007684 STOCK Tg(Atoh1-cre/Esr1*)14Fsh/J 007742 B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J 007915 129S.FVB-Tg(Amh-cre)8815Reb/J 008069 B6;129P2-Pvalbtm1(cre)Arbr/J 008085 B6.Cg-Tg(UBC-cre/ERT2)1Ejb/J 008199 STOCK Tg(dlx6a-cre)1Mekk/J 008205 B6.Cg-Tg(NPHS2-cre)295Lbh/J 008244 FVB.Cg-Tg(tetO-cre)1Jaw/J 008320 B6.129-Leprtm2(cre)Rck/J 008454 B6.Cg-Tg(Sox2-cre)1Amc/J 008533 B6;FVB-Tg(Cspg4-cre)1Akik/J 008537 FVB-Tg(Tek-cre)2352Rwng/J 008538 B6.Cg-Tg(Cspg4-cre/Esr1*)BAkik/J 008582 STOCK Tg(Kcnc2-Cre)K128Stl/LetJ 008601 B6.Cg-Tg(Th-cre)1Tmd/J 008735 B6.Cg-Tg(Wap-cre)11738Mam/JKnwJ 008766 C57BL/6-Tg(Cd8a-cre)1Itan/J 008783 STOCK Smn1tm3(SMN2/Smn1)Mrph Tg(SMN2*delta7)4299Ahmb Tg(SMN2)89Ahmb Tg(CAG-cre/Esr1*)5Amc/J 008839 B6;C3-Tg(Cyp39a1-cre)1Aibs/J 008844 B6;C3-Tg(Ctgf-cre)2Aibs/J 008847 STOCK Tg(Msx2-cre/ERT)27Alj/J 008848 B6;C3-Tg(Mybpc1-cre)2Aibs/J 008851 STOCK Tg(Wnt1-cre/ERT)1Alj/J 008852 STOCK Tg(En2-cre)22Alj/J 008863 B6.Cg-Tg(Tek-cre)1Ywa/J 008870 C57BL/6-Tg(Hspa2-cre)1Eddy/J 009074 STOCK Tg(Myh6-cre)1Jmk/J 009102 STOCK Tg(Nefh-cre)12Kul/J 009103 B6;C3-Tg(Wfs1-cre/ERT2)3Aibs/J 009111 B6;C3-Tg(Scnn1a-cre)1Aibs/J 009117 B6;C3-Tg(Cyp39a1-cre)7Aibs/J 009155 C57BL/6-Cldn6tm1(cre)Dkwu/J 009613 B6;C3-Tg(Scnn1a-cre)3Aibs/J 009614 B6.Cg-Tg(Wfs1-cre/ERT2)2Aibs/J 009615 STOCK Tg(Cartpt-cre)1Aibs/J 009616 B6;C3-Tg(A930038C07Rik-cre)4Aibs/J 009643 B6.Cg-Tg(Lhb-cre)1Sac/J 010536 B6.Cg-Tg(Pcp2-cre)3555Jdhu/J 010714 B6.FVB-Tg(Pomc-cre)1Stl/J 010803 B6;FVB-Tg(Adipoq-cre)1Evdr/J 012328 B6.Cg-Tg(Tyr-cre/ERT2)13Bos/J 012620 STOCK Trp53tm1Brd Brca1tm1Aash Tg(LGB-cre)74Acl/J 012641 BALB/c-Tg(S100a4-cre)1Egn/YunkJ 012686 C57BL/6N-Tg(Ppp1r2-cre)4127Nkza/J 012688 B6(129S4)-Et(cre/ERT2)13866Rdav/J 012712 B6.Cg-Tg(Fev-cre)1Esd/J 012841 STOCK Tg(Ggt1-cre)M3Egn/J 012886 B6.Cg-Tg(Gfap-cre)73.12Mvs/J 012887 B6.Cg-Tg(Gfap-cre)77.6Mvs/J 012906 C57BL/6-Tg(Nes-cre/Esr1*)1Kuan/J 013148 C57BL/6-Tg(Pdgfra-cre)1Clc/J 013233 NOD.B6-Tg(Itgax-cre,-EGFP)4097Ach/J 013234 NOD.Cg-Tg(Cd4-cre)1Cwi/2AchJ 013590 B6.Cg-Braftm1Mmcm Ptentm1Hwu Tg(Tyr-cre/ERT2)13Bos/BosJ 014094 B6N.Cg-Tg(Sox2-cre)1Amc/J 014158 STOCK Tg(Pax4-cre)1Dam/J 014600 STOCK Tg(I12b-cre/ERT2,-ALPP)37Fsh/J 014647 B6.FVB-Tg(Ipfl-cre)6Tuv/J 016097 C57BL/6-Tg(Car1-cre)5Flt/J 016261 C57BL/6-Tg(Nes-cre/ERT2)KEisc/J 017336 STOCK Tg(Cd4-cre)1Cwi/BfluJ 017346 B6.Cg-Tg(A930038C07Rik-cre)1Aibs/J 017491 B6.Cg-Tg(Tagln-cre)1Her/J 017495 B6;129S7-Crim1tm1(GFP/cre/ERT2)Pzg/J 017593 B6;129S-Sox2tm1(cre/ERT2)Hoch/J 017595 FVB.Cg-Tg(CAG-cre/Esr1*)5Amc/J 017738 B6;SJL-Tg(Foxl1-cre)1Khk/J 017769 B6.129-Trpv1tm1(cre)Bbm/J 017947 STOCK Tg(Krt8-cre/ERT2)17Blpn/J 017948 STOCK Tg(KRT18-cre/ERT2)23Blpn/J 017981 STOCK Tg(Hoxb6-cre)#Mku/J 018394 FVB.Cg-Tg(KRT5-cre/ERT2)2Ipc/JeldJ 018829 STOCK Tg(Sox9-cre/ERT2)1Msan/J 018961 B6N.Cg-Tg(Alb-cre)21Mgn/J Genotyping resources and troubleshooting This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.