Stock Number: 003654 Strain Name: B6.129X1-Gad2tm1Bae/J

Allele: Gad2tm1Bae Protocol Name: Gad2tm1Bae STD PCR Method: Standard PCR Version: 1.0 Created: 28-July -2009 Updated: 12-April -2010

Gel Image

no data found

Protocol Primers

Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type oIMR2060 - CAC GAG ACT AGT GAG ACG TG - Neo oIMR3457 - CAT ACG CAG ACA GCA CGT TT - Common oIMR3473 - CAA ACC CTA AAC CAC CCA CA - Wild type Forward

Number of Reactions

Reaction/Components  Reaction Component Volume (µl) Final Concentration Total Volume (µl) ddH2O 3.34 - 3.34 2.5 mM dNTP 0.96 0.20 mM 0.96 5 mM DNA Loading Dye 1.66 0.69 mM 1.66 25 mM MgCl2 0.96 2.00 mM 0.96 DNA 2.00 - 2.00 20 uM oIMR3473 0.60 1.00 uM 0.60 20 uM oIMR3457 0.60 1.00 uM 0.60 20 uM oIMR2060 0.60 1.00 uM 0.60 10 X High Fidelity Buffer 1.20 1.00 X 1.20 5 U/ul High Fidelity Platinum Taq 0.08 0.03 U/ul 0.08

Cycling  Step # Temp °C Time Note 1 94 3 min - 2 94 30 sec - 3 63 1 min - 4 72 1 min - 5 - - Go to step 2-4, 35 times 6 72 2 min - 7 10 - hold

This genotyping protocol is used for the following strains:   Stock Number Strain Name 003654 B6.129X1-Gad2tm1Bae/J Genotyping resources and troubleshooting This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.