Stock Number:005769
Strain Name:B6.129P2-Htr7tm1Dgen/J

Htr7tm1Dgen
Htr7tm1Dgen
Standard PCR
1.1
05-September-2008 11-January -2010
Expected Results: Mutant = 494 bp
Heterozygote = 307 bp and 494 bp
Wild type = 307 bp
Gel Information: Separated by gel electrophoresis on a 1.5% agarose gel.
Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
moIMR0012 - GGG TGG GAT TAG ATA AAT GCC TGC TCT - -
oIMR4384 - CAT GTC GGT CAT GCT GTT CAT GTA C - -
oIMR4385 - TAA AGG GCC TTG CTG TGG ACA AGA G - -
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration
Total Volume (µl)
ddH2O 4.94 - 4.94
10 X AB PCR BufferII 1.00 1.00 X 1.00
50 mM MgCl2 0.30 1.50 mM 0.30
2.5 mM dNTP 0.96 0.24 mM 0.96
20 uM oIMR4384 0.25 0.50 uM 0.25
20 uM oIMR4385 0.25 0.50 uM 0.25
20 uM moIMR0012 0.25 0.50 uM 0.25
5 U/ul TEMPase II Taq Pol. 0.05 0.03 U/ul 0.05
DNA 2.00 - 2.00
Cycling 
Step # Temp °C Time Note
1 95 7 min -
2 94 30 sec -
3 60 30 sec -
4 68 1.5 min repeat steps 2-4 for 35 cycles
5 68 7 min -
6 10 - hold
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
005769 B6.129P2-Htr7tm1Dgen/J
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.