Stock Number: 004939 Strain Name: NOD/ShiLtJ-Leprdb-5J/LtJ

Allele: Leprdb-5J Protocol Name: Leprdb-5J Method: Pyrosequencing Version: 2.1 Created: 29-September-2008 Updated: 24-September-2009

Notes: This genotyping assay uses pyrosequencing technology and is run on the Biotage PSQ 96MA. The Jackson Laboratory is not posting the complete details of our pyrosequencing genotyping assays as the primers for pyrosequencing cannot be used for sequencing using more traditional methods. The wild type and mutant nucleotides and the flanking DNA sequence are provided below. Expected Results: Mutant = T/T Heterozygote = G/T Wild type = G/G

Sequence: CCATTCCTCTGTTGAGGGGCATCTGGGTTCTTTCCAGCTCCTGGCTATTATAAATAAGGC TGCTATGAACATAGTGGAGCATGTGTCCTTCTTACCGGTTGGGACATCTTCTGGATATAT GCCCAGGAGAGGTATTGCTGGATCCTCCGGTAGTACTATGTCCAATTTTCTGAGGAACC GCCAGACTGAGGAACCATAGATGCGAGCATCAGCAACAGAATACAAGAGACTGAAGA CATTTCTCTCGAGAACATGTGTCTGACTCAGGCTTCTTATTGTTTTTCAGTTCCTATGAG AG(g/t)GCCTGAATTTTGGAGAAAAATGGATGGGGACGTTACTAAAAAGGAGAGAAATG TCACCTTGCTTTGGAAGGTATTTCTAATTTCTACCTTTTATTTTATTCTGAACTTATGTTTG TCTCTACTTTGTTCATAGCCCTGTCATTACCTCAGAAATGACCCTCAGATCTTATGTTTTA ATGCGTTTTGGGGATGTGGTTGTCAGAGCTGACTATCTGGAGTATCGACATTGTCAGGG GCCTTTATTATTTTCTCGGTTCAATTATAATTGTGCCGTTGCAGTTTTGGCAAAGTCTTGA ACTCTTTGGACATCACAATTTGTCATCTGTATCAGGGTTAAGATTACTAAGTGATAA

This genotyping protocol is used for the following strains:   Stock Number Strain Name 004939 NOD/ShiLtJ-Leprdb-5J/LtJ Genotyping resources and troubleshooting This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.