Stock Number: 010702 Strain Name: STOCK Gad2tm1(cre/ERT2)Zjh/J

Allele: Generic FLP Protocol Name: Tg(ACTFLPe) Method: High Resolution Melting Version: 3.1 Created: 27-April -2009 Updated: 05-June -2011

Gel Image

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Protocol Primers

Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type oIMR7338 - CTA GGC CAC AGA ATT GAA AGA TCT - Internal Positive Control Forward oIMR7339 - GTA GGT GGA AAT TCT AGC ATC ATC C - Internal Positive Control Reverse oIMR9411 - ACT CCG TTA GGC CCT TCA TT - Forward oIMR9412 - GCG CCT TAT TCA ATC TTT GC - Reverse

Number of Reactions

Reaction/Components  Reaction Component Volume (µl) Final Concentration Total Volume (µl) ddH2O 1.24 - 1.24 2 X High Resolution Melt Master Mix (Roche) 6.00 1.00 X 6.00 25 mM MgCl2 0.96 2.00 mM 0.96 20 uM oIMR9411 0.60 1.00 uM 0.60 20 uM oIMR9412 0.60 1.00 uM 0.60 20 uM oIMR7338 0.30 0.50 uM 0.30 20 uM oIMR7339 0.30 0.50 uM 0.30 DNA 2.00 - 2.00

Cycling  Step # Temp °C Time Note 1 94 10 min - 2 94 30 sec - 3 59 1 min - 4 72 45 sec repeat steps 2-4 for 35 cycles 5 72 2 min - 6 10 - hold

This genotyping protocol is used for the following strains:   Stock Number Strain Name 003800 B6;SJL-Tg(ACTFLPe)9205Dym/J 005703 B6.Cg-Tg(ACTFLPe)9205Dym/J 007844 129S4/SvJae-Gt(ROSA)26Sortm2(FLP*)Sor/J 010702 STOCK Gad2tm1(cre/ERT2)Zjh/J 011081 B6;129-Osmrtm1.1Nat/J 012623 B6;129S4-Carftm1.1Aew/J 012627 B6.Cg-Stard4tm1.1Bres/J 012653 B6;129-Pax7tm1.1Fan/J 012839 B6.129X1(Cg)-Tnfrsf4tm2(cre)Nik/J 012888 129S-Wlstm1.1Lan/J 012897 STOCK Slc32a1tm1Lowl/J 013717 B6.Cg-Slc20a1tm1.2Cmg/J 013749 STOCK Iis2tm1(ACTB-EGFP,-tdTomato)Luo/J 014168 B6;129-Pot1atm1.1Tdl/J 014648 B6.Cg-Gt(ROSA)26Sortm37(H1/tetO-RNAi:Taz)Arte/ZkhuJ 016168 B6;129S4-Fbxl3Gt(FHCRC-GT-S13-12G1)Sor/JtJ 017606 STOCK Hopxtm2.1(cre/ERT2)Joe/J 017890 B6(Cg)-Shank3tm1.2Bux/J 018353 B6;129S4-Ror1tm1.1Meg/J 018397 B6;129S-Gt(ROSA)26Sortm1.1(CAG-COX8A/Dendra2)Dcc/J 018445 B6.129-Mre11atm1.1Rchd/J 019100 B6N.Cg-Tg(ACTFLPe)9205Dym/CjDswJ 021191 B6.Cg-Pvalbtm2.1(FLPe)Hze/J 021198 STOCK Hdctm1.1(cre)Wwis/J 021209 B6;129S1-Kat8tm1Thl/J Genotyping resources and troubleshooting This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.