Stock Number: 006943 Strain Name: B6.129S7-B4galt1tm2Shur/J

Allele: B4galt1tm2Shur Protocol Name: B4galt1tm2Shur Method: Pyrosequencing Version: 1.1 Created: 30-September-2008 Updated: 24-September-2009

Notes: This genotyping assay uses pyrosequencing technology and is run on the Biotage PSQ 96MA. The Jackson Laboratory is not posting the complete details of our pyrosequencing genotyping assays as the primers for pyrosequencing cannot be used for sequencing using more traditional methods. The wild type and mutant nucleotides and the flanking DNA sequence are provided below. Expected Results: Mutant = CTT Wild type = ATG

Sequence: CCCAGGTTCCGCGAACAGCCTTCCGAAACCCGGTTGAGGCCCCGCCCTAGGTCGAG ACCACGCCCCCTGCTCATCCGCGCTTGTGGTTCCCGCTGATCTCTCCAAAGTCCCGC GGCCAGGGGTGTCGGCTTTCCGCCGCAGGCGGCCGGTGCTGCGCTAGGTCCTAGCC CCCTCCCCCCGGCCCCTCCTTCGATCGCTGTGGTCGGGTAGCGCCTGGCGGGCGGC CTGCGGGCGGGCCGTCCTCTCAGCCGTAGCCCACCCCCTCTTAAAGCCGCGGCGG GAAG(atg/ctt)AGGTTTCGTGAGCAGTTCCTGGGCGGCAGCGCCGCGATGCCGGGCGC GACCCTGCAGCGGGCCTGCCGCCTGCTCGTGGCCGTCTGCGCGCTGCACCTCGGCG TCACCCTCGTCTATTACCTCTCTGGCCGCGATCTGAGCCGCCTGCCCCAGTTGGTCG GAGTCTCCTCTACACTGCAGGGCGGCACGAACGGCGCCGCAGCCAGCAAGCAGCC CCCAGGAGAGCAGCGGCCGCGGGGTGCGCGGCCGCCGCCTCCTTTAGGCGTCTCCC CGAAGCCTCGCCCGGGTCTCGACTCCAGCCCTGGTGCAGCTTCTGGCCCCGGCTTG AAGAGCAACTTGTCTTCGTTGCCAGTGCCCACCA

This genotyping protocol is used for the following strains:   Stock Number Strain Name 006943 B6.129S7-B4galt1tm2Shur/J Genotyping resources and troubleshooting This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.