Stock Number: 008651 Strain Name: 129S-Trp53tm3Tyj/J

Allele: Trp53tm1Tyj Protocol Name: Trp53tm3.1Tyj Method: Pyrosequencing Version: 2.0 Created: 18-May -2009 Updated: 24-September-2009

Notes: This genotyping assay uses pyrosequencing technology and is run on the Biotage PSQ 96MA. The Jackson Laboratory is not posting the complete details of our pyrosequencing genotyping assays as the primers for pyrosequencing cannot be used for sequencing using more traditional methods. The wild type and mutant nucleotides and the flanking DNA sequence are provided below. Expected Results: Mutant = A/A Heterozygote = A/G Wild type = G/G

Sequence: CTCCAGGTAGGAAGGCGCGTGGTAGGTTAGGTTAGCCTGTTTCTTCCCCAGCTTC TGCCTGTTTCTGTTCCACGAGTCCCGCCCCCTACCACATGCCCAACGCTCTTTGG TTCCTACCCTATCTACCTAAATGAAGTCTCCTCCTCTGTTTCCTCTTGGGCTTAGG GACGTCTCTTATCTGTGGCTTCTCGGGGTTCCTGTAACTGGACCTTTGGCTGCAG ATATGACAAGAGGGGTTGGGAACAGGTGGGGGCCTAGTTTACACACAGTCAGG ATGGGGCCCAGCTTTCTTACTGCCTTGTGCTGGTCCTTTTCTTGTCCCGGATAGT GGGAACCTTCTGGGACGGGACAGCTTTGAGGTTC(g/a)TGTTTGTGCCTGCCCTGG GAGAGACCGCCGTACAGAAGAAGAAAATTTCCGCAAAAAGGAAGTCCTTTGCC CTGAACTGCCCCCAGGGAGCGCAAAGAGAGGTACGCAGGCGGGACCAAGGAG GCGGAGGAGCCTGTTGAGCTTCACCCCAAAGTCACCTCTTGCTCTCTCCTTCCA CAGCGCTGCCCACCTGCACAAGCGCCTCTCCCCCGCAAAAGAAAAAACCACTT GATGGAGAGTATTTCACCCTCAAGGTACCAAGGCTGGAGAGTCGCATGCCAGA GACAAGCTGTACCCATTATTGCCTCTGTCTCTCGCATGTATAAAATAGTGTTATT AGCAGGTTGCCAG

This genotyping protocol is used for the following strains:   Stock Number Strain Name 008182 B6.129S4-Trp53tm3.1Tyj/J 008651 129S-Trp53tm3Tyj/J Genotyping resources and troubleshooting This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.