Stock Number:000058
Strain Name:B6(Cg)-Tyrc-2J/J
Tyrc-2J
Tyrc-2J alternative 1
End Point Analysis
1.0
27-January -2012 17-February -2012
Expected Results:

Mutant = T

Wild type = G
Sequence:

ATGTTCTTGGCTGTTTTGTATTGCCTTCTGTGGAGTTTCCAGATCTCTGATGGCCATTTTCCTCGAGCCTGTGCCTCCTCTAAGAACTTGTTGGCAAAAGAATGCTGCCCACCATGGATGGGTGATGGGAGTCCCTGCGGCCAGCTTTCAGGCAGAGGTTCCTGCCAGGATATCCTTCTGTCCAGTGCACCATCTGGACCTCAGTTCCCCTTCAAAGGGGTGGATGACC[g/t]TGAGTCCTGGCCCTCTGTGTTTTATAATAGGACCTGCCAGTGCTCAGGCAACTTCATGGGTTTCAACTGCGGAAACTGTAAGTTTGGATTTGGGGGCCCAAATTGTACAGAGAAGCGAGTCTTGATTAGAAGAAACATTTTTGATTTGAGTGTCTCCGAAAAGAATAAGTTCTTTTCTTACCTCACTTTAGCAAAACATACTATCAGCTCAGTCTATGTCATCCCCACAGGCACCTATGGCCAAATGAACAATGGGTCAACACCCATGTTTAATGATATCAACATCTACGACCTCTTTGTATGGATGCATTACTATGTGTCAAGGGACACACTGCTTGGGGGCTCTGAAATATGGAGGGACATTGATTTTGCCCATGAAGCACCAGGGTTTCTGCCTTGGCACAGACTTTTCTTGTTATTGTGGGAACAAGAAATTCGAGAACTAACTGGGGATGAGAACTTCACTGTT
Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
13969 - GAC CTC AGT TCC CCT TCA AAG - Forward
13970 - ACC CAT GAA GTT GCC TGA G - Reverse
13971 6-FAM GTG GAT GAC CTT GAG TCC TGG

Iowa Black

 Probe
13972 JOE NHS Ester GTG GAT GAC CGT GAG TCC TGG

Iowa Black

 Probe
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
2 X Kapa Probe Fast QPCR 2.50 1.00 X 2.50
ddH2O 0.07 - 0.07
100 uM 13969 0.02 0.40 uM 0.02
100 uM 13970 0.02 0.40 uM 0.02
100 uM 13971 0.01 0.20 uM 0.01
100 uM 13972 0.01 0.20 uM 0.01
DNA 2.37 - 2.37
Cycling 
Step # Temp °C Time Note
1 95 10min -
2 92 15sec -
3 60 1 min -
4 - - repeat steps 2-3 for 40 cycles
5 4 - Forever
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
000058 B6(Cg)-Tyrc-2J/J
007484 B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
013174 C57BL/6-Grntm1Aidi/J
014601 B6(Cg)-Noxa1tm1Brg/BrgJ
017614 B6(Cg)-Tyrc-2J Tg(UBC-mCherry)1Phbs/J
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.