Stock Number:009061
Strain Name:STOCK Osr1tm1(EGFP/cre/ERT2)Amc/J
Osr1tm1(EGFP/cre/ERT2)Amc
Osr1tm1(EGFP/cre/ERT2)Amc SEP PCR
Separated PCR
2.0
15-December -2010 15-December -2010
Expected Results: TGS   WT=353bp
           Mut~650bp                 SC 9/25/09

NOTE: This assay works well using KAPA Long Range Taq - double the amount of KAPA LR Buffer & KAPA LR Taq using less ddH2O.
Gel Image 
no data found
Protocol Primers 
Primer 5' Label Sequence 5' --> 3' 3' Label Primer Type
8139 - CCC TCT TCC TGT CTT TGC AG - -
oIMR9677 - GGC TTA GGG TGA ATG ACG TG - -
oIMR9678 - ACC CGT GAT ATT GCT GAA GAG CTT - -
oIMR9679 - TGA AGA GCG CTG AAA CCA TAC A - -
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
20 uM oIMR9677 0.60 1.00 uM 0.60
5 X KAPA LR HotStart Buffer 2.40 1.00 X 2.40
ddH2O 2.66 - 2.66
2.5 mM dNTP 0.96 0.20 mM 0.96
5 mM DNA Loading Dye 1.66 0.69 mM 1.66
25 mM MgCl2 0.96 2.00 mM 0.96
20 uM 8139 0.60 1.00 uM 0.60
2.5 U/ul KAPA LR HotStart Taq 0.16 0.03 U/ul 0.16
DNA 2.00 - 2.00
Cycling 
Step # Temp °C Time Note
1 94 3 min -
2 94 30 sec -
3 58 1 min -
4 72 1 min -
5 - - Go to step 2-4, 35 times
6 72 2 min -
7 10 - hold
Reaction/Components 
Reaction Component Volume (µl) Final
Concentration		 Total Volume (µl)
ddH2O 2.66 - 2.66
5 X KAPA LR HotStart Buffer 2.40 1.00 X 2.40
25 mM MgCl2 0.96 2.00 mM 0.96
2.5 mM dNTP 0.96 0.20 mM 0.96
20 uM oIMR9678 0.60 1.00 uM 0.60
20 uM oIMR9679 0.60 1.00 uM 0.60
5 mM DNA Loading Dye 1.66 0.69 mM 1.66
2.5 U/ul KAPA LR HotStart Taq 0.16 0.03 U/ul 0.16
DNA 2.00 - 2.00
This genotyping protocol is used for the following strains:
 
Stock Number Strain Name
009061 STOCK Osr1tm1(EGFP/cre/ERT2)Amc/J
Genotyping resources and troubleshooting
This page contains guidelines, frequently asked questions, and helpful external links to genotyping and PCR resources.
Cycling 
Step # Temp °C Time Note
1 94 3 min -
2 94 30 sec -
3 58 1 min -
4 72 1 min -
5 - - Go to step 2-4, 35 times
6 72 2 min -
7 10 - hold